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pCMV-SpRY-ABE8e

产品货号:P62827

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pCMV-SpRY-ABE8e
别称: Plasmid&#185671
基因长: 4695bp
质粒宿主: 哺乳细胞
质粒用途: 基因编辑
片段类型: CRISPR
片段物种:
原核抗性: Amp
筛选标记:
荧光标记:
启动子: CMV
复制子: pUC
拷贝数:
感受态: DH5a
温度: 37度
背景载体: pCMV with a BR322 origin
正向引物: CMV-F
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

GGAGGTCGCT

GAGTAGTGCG

CGAGCAAAAT

TTAAGCTACA

ACAAGGCAAG

GCTTGACCGA

61

CAATTGCATG

AAGAATCTGC

TTAGGGTTAG

GCGTTTTGCG

CTGCTTCGCG

ATGTACGGGC

121

CAGATATACG

CGTTGACATT

GATTATTGAC

TAGTTATTAA

TAGTAATCAA

TTACGGGGTC

181

ATTAGTTCAT

AGCCCATATA

TTGAGTTCCG

CGTTACATAA

CTTACGGTAA

ATGGCCCGCC

241

TGGCTGACCG

CCCAACGACC

CCCGCCCATT

GACGTCAATA

ATGACGTATG

TTCCCATAGT

301

AACGCCAATA

GGGACTTTCC

ATTGACGTCA

ATGGGTGGAG

TATTTACGGT

AAACTGCCCA

361

CTTGGCAGTA

CATCAAGTGT

ATCATATGCC

AAGTACGCCC

CCTATTGACG

TCAATGACGG

421

TAAATGGCCC

GCCTGGCATT

ATGCCCAGTA

CATGACCTTA

TGGGACTTTC

CTACTTGGCA

481

GTACATCTAC

GTATTAGTCA

TCGCTATTAC

CATGGTGATG

CGGTTTTGGC

AGTACATCAA

541

TGGGCGTGGA

TAGCGGTTTG

ACTCACGGGG

ATTTCCAAGT

CTCCACCCCA

TTGACGTCAA

601

TGGGAGTTTG

TTTTGGCACC

AAAATCAACG

GGACTTTCCA

AAATGTCGTA

ACAACTCCGC

661

CCCATTGACG

CAAATGGGCG

GTAGGCGTGT

ACGGTGGGAG

GTCTATATAA

GCAGAGCTGG

721

TTTAGTGAAC

CGTCAGATCC

GCTAGAGATC

CGCGGCCGCT

AATACGACTC

ACTATAGGGA

781

GAGCCGCCAC

CATGAAACGG

ACAGCCGACG

GAAGCGAGTT

CGAGTCACCA

AAGAAGAAGC

841

GGAAAGTCTC

TGAGGTGGAG

TTTTCCCACG

AGTACTGGAT

GAGACATGCC

CTGACCCTGG

901

CCAAGAGGGC

ACGGGATGAG

AGGGAGGTGC

CTGTGGGAGC

CGTGCTGGTG

CTGAACAATA

961

GAGTGATCGG

CGAGGGCTGG

AACAGAGCCA

TCGGCCTGCA

CGACCCAACA

GCCCATGCCG

1021

AAATTATGGC

CCTGAGACAG

GGCGGCCTGG

TCATGCAGAA

CTACAGACTG

ATTGACGCCA

1081

CCCTGTACGT

GACATTCGAG

CCTTGCGTGA

TGTGCGCCGG

CGCCATGATC

CACTCTAGGA

1141

TCGGCCGCGT

GGTGTTTGGC

GTGAGGAACT

CAAAAAGAGG

CGCCGCAGGC

TCCCTGATGA

1201

ACGTGCTGAA

CTACCCCGGC

ATGAATCACC

GCGTCGAAAT

TACCGAGGGA

ATCCTGGCAG

1261

ATGAATGTGC

CGCCCTGCTG

TGCGATTTCT

ATCGGATGCC

TAGACAGGTG

TTCAATGCTC

1321

AGAAGAAGGC

CCAGAGCTCC

ATCAACTCTG

GAGGATCTAG

CGGAGGATCC

TCTGGCAGCG

1381

AGACACCAGG

AACAAGCGAG

TCAGCAACAC

CAGAGAGCAG

TGGCGGCAGC

AGCGGCGGCA

1441

GCGACAAGAA

GTACAGCATC

GGCCTGGCCA

TCGGCACCAA

CTCTGTGGGC

TGGGCCGTGA

1501

TCACCGACGA

GTACAAGGTG

CCCAGCAAGA

AATTCAAGGT

GCTGGGCAAC

ACCGACCGGC

1561

ACAGCATCAA

GAAGAACCTG

ATCGGAGCCC

TGCTGTTCGA

CAGCGGCGAA

ACAGCCGAGA

1621

GAACCCGGCT

GAAGAGAACC

GCCAGAAGAA

GATACACCAG

ACGGAAGAAC

CGGATCTGCT

1681

ATCTGCAAGA

GATCTTCAGC

AACGAGATGG

CCAAGGTGGA

CGACAGCTTC

TTCCACAGAC

1741

TGGAAGAGTC

CTTCCTGGTG

GAAGAGGATA

AGAAGCACGA

GCGGCACCCC

ATCTTCGGCA

1801

ACATCGTGGA

CGAGGTGGCC

TACCACGAGA

AGTACCCCAC

CATCTACCAC

CTGAGAAAGA

1861

AACTGGTGGA

CAGCACCGAC

AAGGCCGACC

TGCGGCTGAT

CTATCTGGCC

CTGGCCCACA

1921

TGATCAAGTT

CCGGGGCCAC

TTCCTGATCG

AGGGCGACCT

GAACCCCGAC

AACAGCGACG

1981

TGGACAAGCT

GTTCATCCAG

CTGGTGCAGA

CCTACAACCA

GCTGTTCGAG

GAAAACCCCA

2041

TCAACGCCAG

CGGCGTGGAC

GCCAAGGCCA

TCCTGTCTGC

CAGACTGAGC

AAGAGCAGAC

2101

GGCTGGAAAA

TCTGATCGCC

CAGCTGCCCG

GCGAGAAGAA

GAATGGCCTG

TTCGGAAACC

2161

TGATTGCCCT

GAGCCTGGGC

CTGACCCCCA

ACTTCAAGAG

CAACTTCGAC

CTGGCCGAGG

2221

ATGCCAAACT

GCAGCTGAGC

AAGGACACCT

ACGACGACGA

CCTGGACAAC

CTGCTGGCCC

2281

AGATCGGCGA

CCAGTACGCC

GACCTGTTTC

TGGCCGCCAA

GAACCTGTCC

GACGCCATCC

2341

TGCTGAGCGA

CATCCTGAGA

GTGAACACCG

AGATCACCAA

GGCCCCCCTG

AGCGCCTCTA

2401

TGATCAAGAG

ATACGACGAG

CACCACCAGG

ACCTGACCCT

GCTGAAAGCT

CTCGTGCGGC

2461

AGCAGCTGCC

TGAGAAGTAC

AAAGAGATTT

TCTTCGACCA

GAGCAAGAAC

GGCTACGCCG

2521

GCTACATTGA

CGGCGGAGCC

AGCCAGGAAG

AGTTCTACAA

GTTCATCAAG

CCCATCCTGG

2581

AAAAGATGGA

CGGCACCGAG

GAACTGCTCG

TGAAGCTGAA

CAGAGAGGAC

CTGCTGCGGA

2641

AGCAGCGGAC

CTTCGACAAC

GGCAGCATCC

CCCACCAGAT

CCACCTGGGA

GAGCTGCACG

2701

CCATTCTGCG

GCGGCAGGAA

GATTTTTACC

CATTCCTGAA

GGACAACCGG

GAAAAGATCG

2761

AGAAGATCCT

GACCTTCCGC

ATCCCCTACT

ACGTGGGCCC

TCTGGCCAGG

GGAAACAGCA

2821

GATTCGCCTG

GATGACCAGA

AAGAGCGAGG

AAACCATCAC

CCCCTGGAAC

TTCGAGGAAG

2881

TGGTGGACAA

GGGCGCTTCC

GCCCAGAGCT

TCATCGAGCG

GATGACCAAC

TTCGATAAGA

2941

ACCTGCCCAA

CGAGAAGGTG

CTGCCCAAGC

ACAGCCTGCT

GTACGAGTAC

TTCACCGTGT

3001

ATAACGAGCT

GACCAAAGTG

AAATACGTGA

CCGAGGGAAT

GAGAAAGCCC

GCCTTCCTGA

3061

GCGGCGAGCA

GAAAAAGGCC

ATCGTGGACC

TGCTGTTCAA

GACCAACCGG

AAAGTGACCG

3121

TGAAGCAGCT

GAAAGAGGAC

TACTTCAAGA

AAATCGAGTG

CTTCGACTCC

GTGGAAATCT

3181

CCGGCGTGGA

AGATCGGTTC

AACGCCTCCC

TGGGCACATA

CCACGATCTG

CTGAAAATTA

3241

TCAAGGACAA

GGACTTCCTG

GACAATGAGG

AAAACGAGGA

CATTCTGGAA

GATATCGTGC

3301

TGACCCTGAC

ACTGTTTGAG

GACAGAGAGA

TGATCGAGGA

ACGGCTGAAA

ACCTATGCCC

3361

ACCTGTTCGA

CGACAAAGTG

ATGAAGCAGC

TGAAGCGGCG

GAGATACACC

GGCTGGGGCA

3421

GGCTGAGCCG

GAAGCTGATC

AACGGCATCC

GGGACAAGCA

GTCCGGCAAG

ACAATCCTGG

3481

ATTTCCTGAA

GTCCGACGGC

TTCGCCAACA

GAAACTTCAT

GCAGCTGATC

CACGACGACA

3541

GCCTGACCTT

TAAAGAGGAC

ATCCAGAAAG

CCCAGGTGTC

CGGCCAGGGC

GATAGCCTGC

3601

ACGAGCACAT

TGCCAATCTG

GCCGGCAGCC

CCGCCATTAA

GAAGGGCATC

CTGCAGACAG

3661

TGAAGGTGGT

GGACGAGCTC

GTGAAAGTGA

TGGGCCGGCA

CAAGCCCGAG

AACATCGTGA

3721

TCGAAATGGC

CAGAGAGAAC

CAGACCACCC

AGAAGGGACA

GAAGAACAGC

CGCGAGAGAA

3781

TGAAGCGGAT

CGAAGAGGGC

ATCAAAGAGC

TGGGCAGCCA

GATCCTGAAA

GAACACCCCG

3841

TGGAAAACAC

CCAGCTGCAG

AACGAGAAGC

TGTACCTGTA

CTACCTGCAG

AATGGGCGGG

3901

ATATGTACGT

GGACCAGGAA

CTGGACATCA

ACCGGCTGTC

CGACTACGAT

GTGGACCATA

3961

TCGTGCCTCA

GAGCTTTCTG

AAGGACGACT

CCATCGACAA

CAAGGTGCTG

ACCAGAAGCG

4021

ACAAGAACCG

GGGCAAGAGC

GACAACGTGC

CCTCCGAAGA

GGTCGTGAAG

AAGATGAAGA

4081

ACTACTGGCG

GCAGCTGCTG

AACGCCAAGC

TGATTACCCA

GAGAAAGTTC

GACAATCTGA

4141

CCAAGGCCGA

GAGAGGCGGC

CTGAGCGAAC

TGGATAAGGC

CGGCTTCATC

AAGAGACAGC

4201

TGGTGGAAAC

CCGGCAGATC

ACAAAGCACG

TGGCACAGAT

CCTGGACTCC

CGGATGAACA

4261

CTAAGTACGA

CGAGAATGAC

AAGCTGATCC

GGGAAGTGAA

AGTGATCACC

CTGAAGTCCA

4321

AGCTGGTGTC

CGATTTCCGG

AAGGATTTCC

AGTTTTACAA

AGTGCGCGAG

ATCAACAACT

4381

ACCACCACGC

CCACGACGCC

TACCTGAACG

CCGTCGTGGG

AACCGCCCTG

ATCAAAAAGT

4441

ACCCTAAGCT

GGAAAGCGAG

TTCGTGTACG

GCGACTACAA

GGTGTACGAC

GTGCGGAAGA

4501

TGATCGCCAA

GAGCGAGCAG

GAAATCGGCA

AGGCTACCGC

CAAGTACTTC

TTCTACAGCA

4561

ACATCATGAA

CTTTTTCAAG

ACCGAGATTA

CCCTGGCCAA

CGGCGAGATC

CGGAAGCGGC

4621

CTCTGATCGA

GACAAACGGC

GAAACCGGGG

AGATCGTGTG

GGATAAGGGC

CGGGATTTTG

4681

CCACCGTGCG

GAAAGTGCTG

AGCATGCCCC

AAGTGAATAT

CGTGAAAAAG

ACCGAGGTGC

4741

AGACAGGCGG

CTTCAGCAAA

GAGTCTATCA

GACCCAAGAG

GAACAGCGAT

AAGCTGATCG

4801

CCAGAAAGAA

GGACTGGGAC

CCTAAGAAGT

ACGGCGGCTT

CCTGTGGCCC

ACCGTGGCCT

4861

ATTCTGTGCT

GGTGGTGGCC

AAAGTGGAAA

AGGGCAAGTC

CAAGAAACTG

AAGAGTGTGA

4921

AAGAGCTGCT

GGGGATCACC

ATCATGGAAA

GAAGCAGCTT

CGAGAAGAAT

CCCATCGACT

4981

TTCTGGAAGC

CAAGGGCTAC

AAAGAAGTGA

AAAAGGACCT

GATCATCAAG

CTGCCTAAGT

5041

ACTCCCTGTT

CGAGCTGGAA

AACGGCCGGA

AGAGAATGCT

GGCCTCTGCC

AAGCAGCTGC

5101

AGAAGGGAAA

CGAACTGGCC

CTGCCCTCCA

AATATGTGAA

CTTCCTGTAC

CTGGCCAGCC

5161

ACTATGAGAA

GCTGAAGGGC

TCCCCCGAGG

ATAATGAGCA

GAAACAGCTG

TTTGTGGAAC

5221

AGCACAAGCA

CTACCTGGAC

GAGATCATCG

AGCAGATCAG

CGAGTTCTCC

AAGAGAGTGA

5281

TCCTGGCCGA

CGCTAATCTG

GACAAAGTGC

TGTCCGCCTA

CAACAAGCAC

CGGGATAAGC

5341

CCATCAGAGA

GCAGGCCGAG

AATATCATCC

ACCTGTTTAC

CCTGACCAGA

CTGGGAGCCC

5401

CTAGAGCCTT

CAAGTACTTT

GACACCACCA

TCGACCCCAA

GCAGTACAGA

AGCACCAAAG

5461

AGGTGCTGGA

CGCCACCCTG

ATCCACCAGA

GCATCACCGG

CCTGTACGAG

ACACGGATCG

5521

ACCTGTCTCA

GCTGGGAGGT

GACTCTGGCG

GCTCAAAAAG

AACCGCCGAC

GGCAGCGAAT

5581

TCGAGCCCAA

GAAGAAGAGG

AAAGTCTAAC

CGGTCATCAT

CACCATCACC

ATTGAGTTTA

5641

AACCCGCTGA

TCAGCCTCGA

CTGTGCCTTC

TAGTTGCCAG

CCATCTGTTG

TTTGCCCCTC

5701

CCCCGTGCCT

TCCTTGACCC

TGGAAGGTGC

CACTCCCACT

GTCCTTTCCT

AATAAAATGA

5761

GGAAATTGCA

TCGCATTGTC

TGAGTAGGTG

TCATTCTATT

CTGGGGGGTG

GGGTGGGGCA

5821

GGACAGCAAG

GGGGAGGATT

GGGAAGACAA

TAGCAGGCAT

GCTGGGGATG

CGGTGGGCTC

5881

TATGGCTTCT

GAGGCGGAAA

GAACCAGCTG

GGGCTCGATA

CCGTCGACCT

CTAGCTAGAG

5941

CTTGGCGTAA

TCATGGTCAT

AGCTGTTTCC

TGTGTGAAAT

TGTTATCCGC

TCACAATTCC

6001

ACACAACATA

CGAGCCGGAA

GCATAAAGTG

TAAAGCCTAG

GGTGCCTAAT

GAGTGAGCTA

6061

ACTCACATTA

ATTGCGTTGC

GCTCACTGCC

CGCTTTCCAG

TCGGGAAACC

TGTCGTGCCA

6121

GCTGCATTAA

TGAATCGGCC

AACGCGCGGG

GAGAGGCGGT

TTGCGTATTG

GGCGCTCTTC

6181

CGCTTCCTCG

CTCACTGACT

CGCTGCGCTC

GGTCGTTCGG

CTGCGGCGAG

CGGTATCAGC

6241

TCACTCAAAG

GCGGTAATAC

GGTTATCCAC

AGAATCAGGG

GATAACGCAG

GAAAGAACAT

6301

GTGAGCAAAA

GGCCAGCAAA

AGGCCAGGAA

CCGTAAAAAG

GCCGCGTTGC

TGGCGTTTTT

6361

CCATAGGCTC

CGCCCCCCTG

ACGAGCATCA

CAAAAATCGA

CGCTCAAGTC

AGAGGTGGCG

6421

AAACCCGACA

GGACTATAAA

GATACCAGGC

GTTTCCCCCT

GGAAGCTCCC

TCGTGCGCTC

6481

TCCTGTTCCG

ACCCTGCCGC

TTACCGGATA

CCTGTCCGCC

TTTCTCCCTT

CGGGAAGCGT

6541

GGCGCTTTCT

CATAGCTCAC

GCTGTAGGTA

TCTCAGTTCG

GTGTAGGTCG

TTCGCTCCAA

6601

GCTGGGCTGT

GTGCACGAAC

CCCCCGTTCA

GCCCGACCGC

TGCGCCTTAT

CCGGTAACTA

6661

TCGTCTTGAG

TCCAACCCGG

TAAGACACGA

CTTATCGCCA

CTGGCAGCAG

CCACTGGTAA

6721

CAGGATTAGC

AGAGCGAGGT

ATGTAGGCGG

TGCTACAGAG

TTCTTGAAGT

GGTGGCCTAA

6781

CTACGGCTAC

ACTAGAAGAA

CAGTATTTGG

TATCTGCGCT

CTGCTGAAGC

CAGTTACCTT

6841

CGGAAAAAGA

GTTGGTAGCT

CTTGATCCGG

CAAACAAACC

ACCGCTGGTA

GCGGTGGTTT

6901

TTTTGTTTGC

AAGCAGCAGA

TTACGCGCAG

AAAAAAAGGA

TCTCAAGAAG

ATCCTTTGAT

6961

CTTTTCTACG

GGGTCTGACA

CTCAGTGGAA

CGAAAACTCA

CGTTAAGGGA

TTTTGGTCAT

7021

GAGATTATCA

AAAAGGATCT

TCACCTAGAT

CCTTTTAAAT

TAAAAATGAA

GTTTTAAATC

7081

AATCTAAAGT

ATATATGAGT

AAACTTGGTC

TGACAGTTAC

CAATGCTTAA

TCAGTGAGGC

7141

ACCTATCTCA

GCGATCTGTC

TATTTCGTTC

ATCCATAGTT

GCCTGACTCC

CCGTCGTGTA

7201

GATAACTACG

ATACGGGAGG

GCTTACCATC

TGGCCCCAGT

GCTGCAATGA

TACCGCGAGA

7261

CCCACGCTCA

CCGGCTCCAG

ATTTATCAGC

AATAAACCAG

CCAGCCGGAA

GGGCCGAGCG

7321

CAGAAGTGGT

CCTGCAACTT

TATCCGCCTC

CATCCAGTCT

ATTAATTGTT

GCCGGGAAGC

7381

TAGAGTAAGT

AGTTCGCCAG

TTAATAGTTT

GCGCAACGTT

GTTGCCATTG

CTACAGGCAT

7441

CGTGGTGTCA

CGCTCGTCGT

TTGGTATGGC

TTCATTCAGC

TCCGGTTCCC

AACGATCAAG

7501

GCGAGTTACA

TGATCCCCCA

TGTTGTGCAA

AAAAGCGGTT

AGCTCCTTCG

GTCCTCCGAT

7561

CGTTGTCAGA

AGTAAGTTGG

CCGCAGTGTT

ATCACTCATG

GTTATGGCAG

CACTGCATAA

7621

TTCTCTTACT

GTCATGCCAT

CCGTAAGATG

CTTTTCTGTG

ACTGGTGAGT

ACTCAACCAA

7681

GTCATTCTGA

GAATAGTGTA

TGCGGCGACC

GAGTTGCTCT

TGCCCGGCGT

CAATACGGGA

7741

TAATACCGCG

CCACATAGCA

GAACTTTAAA

AGTGCTCATC

ATTGGAAAAC

GTTCTTCGGG

7801

GCGAAAACTC

TCAAGGATCT

TACCGCTGTT

GAGATCCAGT

TCGATGTAAC

CCACTCGTGC

7861

ACCCAACTGA

TCTTCAGCAT

CTTTTACTTT

CACCAGCGTT

TCTGGGTGAG

CAAAAACAGG

7921

AAGGCAAAAT

GCCGCAAAAA

AGGGAATAAG

GGCGACACGG

AAATGTTGAA

TACTCATACT

7981

CTTCCTTTTT

CAATATTATT

GAAGCATTTA

TCAGGGTTAT

TGTCTCATGA

GCGGATACAT

8041

ATTTGAATGT

ATTTAGAAAA

ATAAACAAAT

AGGGGTTCCG

CGCACATTTC

CCCGAAAAGT

8101

GCCACCTGAC

GTCGACGGAT

CGGGAGATCG

ATCTCCCGAT

CCCCTAGGGT

CGACTCTCAG

8161

TACAATCTGC

TCTGATGCCG

CATAGTTAAG

CCAGTATCTG

CTCCCTGCTT

GTGTGTT

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