名称: | pGD-γb |
---|---|
别称: | Plasmid𯻧 |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 病毒 |
原核抗性: | Kan |
筛选标记: | |
荧光标记: | |
启动子: | 35S |
复制子: | pVS1 |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pGD |
正向引物: | 35S |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CTGGTGGCAG
GATATATTGT
GGTGTAAACA
AATTGACGCT
TAGACAACTT
AATAACACAT
TGCGGACGTT
TTTAATGTAC
TGAATTAACG
CCGAATTAAT
ACGCGTCATG
GTGGAGCACG
ACACTCTTGT
CTACTCCAAA
AATATCAAAG
ATACAGTCTC
AGAAGACCAA
AGGGCAATTG
AGACTTTTCA
ACAAAGGGTA
ATATCCGGAA
ACCTCCTCGG
ATTCCATTGC
CCAGCTATCT
GTCACTTTAT
TGTGAAGATA
GTGGAAAAGG
AAGGTGGCTC
CTACAAATGC
CATCATTGCG
ATAAAGGAAA
GGCCATCGTT
GAAGATGCCT
CTGCCGACAG
TGGTCCCAAA
GATGGACCCC
CACCCACGAG
GAGCATCGTG
GAAAAAGAAG
ACGTTCCAAC
CACGTCTTCA
AAGCAAGTGG
ATTGATGTGA
TATCTCCACT
GACGTAAGGG
ATGACGCACA
ATCCCACTAT
CCTTCGCAAG
ACCCTTCCTC
TATATAAGGA
AGTTCATTTC
ATTTGGAGAG
GACACGCTGA
AATCACCAGT
CTCTCTCTAC
AAGATCTCGA
GATGATGGCT
ACTTTCTCTT
GTGTGTGTTG
TGGTACCTTA
ACTACAAGTA
CTTACTGTGG
TAAGAGATGT
GAGCGAAAGC
ATGTATATTC
TGAAACAAGA
AATAAGAGAT
TGGAACTTTA
CAAGAAGTAT
CTATTGGAAC
CGCAAAAATG
CGCCCTGAAT
GGAATCGTTG
GACACAGTTG
TGGAATGCCA
TGCTCCATTG
CGGAAGAGGC
TTGTGATCAA
CTGCCAATCG
TGAGTAGGTT
CTGTGGCCAA
AAGCATGCGG
ATCTGTATGA
TTCACTTCTG
AAACGTTCTG
AACAGGAGTT
ACTTCTTGAA
TTTCTCCAGA
AGAAGATGCA
GGAGCTGAAA
CTTTCTCATA
TCGTAAAAAT
GGCTAAGCTT
GAAAGTGAGG
TTAACGCAAT
ACGTAAGTCC
GTAGCTTCTT
CTTTTGAAGA
TTCTGTTGGA
TGTGATGATT
CTTCTTCCGT
TTCTAAGTAA
AAAAAAAAAA
AAAATGTTTG
ATCAGATCAT
TCAAATCTGA
TGGTGCCCAT
CAACCATATG
ATGGGAGTGT
TTGCACTGCA
GTCGACGGTA
CCGCGGGCCC
GGGATCCACC
GGATCTAGAT
AACTGATCGT
CGACAGAGCT
CGTTCAAACA
TTTGGCAATA
AAGTTTCTTA
AGATTGAATC
CTGTTGCCGG
TCTTGCGATG
ATTATCATAT
AATTTCTGTT
GAATTACGTT
AAGCATGTAA
TAATTAACAT
GTAATGCATG
ACGTTATTTA
TGAGATGGGT
TTTTATGATT
AGAGTCCCGC
AATTATACAT
TTAATACGCG
ATAGAAAACA
AAATATAGCG
CGCAAACTAG
GATAAATTAT
CGCGCGCGGT
GTCATCTATG
TTACTAGATC
GGGAATTAAA
CTATCAGTGT
TTGACAGGAT
ATATTGGCGG
GTAAACCTAA
GAGAAAAGAG
CGTTTATTAG
AATAATCGGA
TATTTAAAAG
GGCGTGAAAA
GGTTTATCCG
TTCGTCCATT
TGTATGTGCA
TGCCAACCAC
AGGGTTCCCC
TCGGGATCAA
AGTACTTTGA
TCCAACCCCT
CCGCTGCTAT
AGTGCAGTCG
GCTTCTGACG
TTCAGTGCAG
CCGTCTTCTG
AAAACGACAT
GTCGCACAAG
TCCTAAGTTA
CGCGACAGGC
TGCCGCCCTG
CCCTTTTCCT
GGCGTTTTCT
TGTCGCGTGT
TTTAGTCGCA
TAAAGTAGAA
TACTTGCGAC
TAGAACCGGA
GACATTACGC
CATGAACAAG
AGCGCCGCCG
CTGGCCTGCT
GGGCTATGCC
CGCGTCAGCA
CCGACGACCA
GGACTTGACC
AACCAACGGG
CCGAACTGCA
CGCGGCCGGC
TGCACCAAGC
TGTTTTCCGA
GAAGATCACC
GGCACCAGGC
GCGACCGCCC
GGAGCTGGCC
AGGATGCTTG
ACCACCTACG
CCCTGGCGAC
GTTGTGACAG
TGACCAGGCT
AGACCGCCTG
GCCCGCAGCA
CCCGCGACCT
ACTGGACATT
GCCGAGCGCA
TCCAGGAGGC
CGGCGCGGGC
CTGCGTAGCC
TGGCAGAGCC
GTGGGCCGAC
ACCACCACGC
CGGCCGGCCG
CATGGTGTTG
ACCGTGTTCG
CCGGCATTGC
CGAGTTCGAG
CGTTCCCTAA
TCATCGACCG
CACCCGGAGC
GGGCGCGAGG
CCGCCAAGGC
CCGAGGCGTG
AAGTTTGGCC
CCCGCCCTAC
CCTCACCCCG
GCACAGATCG
CGCACGCCCG
CGAGCTGATC
GACCAGGAAG
GCCGCACCGT
GAAAGAGGCG
GCTGCACTGC
TTGGCGTGCA
TCGCTCGACC
CTGTACCGCG
CACTTGAGCG
CAGCGAGGAA
GTGACGCCCA
CCGAGGCCAG
GCGGCGCGGT
GCCTTCCGTG
AGGACGCATT
GACCGAGGCC
GACGCCCTGG
CGGCCGCCGA
GAATGAACGC
CAAGAGGAAC
AAGCATGAAA
CCGCACCAGG
ACGGCCAGGA
CGAACCGTTT
TTCATTACCG
AAGAGATCGA
GGCGGAGATG
ATCGCGGCCG
GGTACGTGTT
CGAGCCGCCC
GCGCACGTCT
CAACCGTGCG
GCTGCATGAA
ATCCTGGCCG
GTTTGTCTGA
TGCCAAGCTG
GCGGCCTGGC
CGGCCAGCTT
GGCCGCTGAA
GAAACCGAGC
GCCGCCGTCT
AAAAAGGTGA
TGTGTATTTG
AGTAAAACAG
CTTGCGTCAT
GCGGTCGCTG
CGTATATGAT
GCGATGAGTA
AATAAACAAA
TACGCAAGGG
GAACGCATGA
AGGTTATCGC
TGTACTTAAC
CAGAAAGGCG
GGTCAGGCAA
GACGACCATC
GCAACCCATC
TAGCCCGCGC
CCTGCAACTC
GCCGGGGCCG
ATGTTCTGTT
AGTCGATTCC
GATCCCCAGG
GCAGTGCCCG
CGATTGGGCG
GCCGTGCGGG
AAGATCAACC
GCTAACCGTT
GTCGGCATCG
ACCGCCCGAC
GATTGACCGC
GACGTGAAGG
CCATCGGCCG
GCGCGACTTC
GTAGTGATCG
ACGGAGCGCC
CCAGGCGGCG
GACTTGGCTG
TGTCCGCGAT
CAAGGCAGCC
GACTTCGTGC
TGATTCCGGT
GCAGCCAAGC
CCTTACGACA
TATGGGCCAC
CGCCGACCTG
GTGGAGCTGG
TTAAGCAGCG
CATTGAGGTC
ACGGATGGAA
GGCTACAAGC
GGCCTTTGTC
GTGTCGCGGG
CGATCAAAGG
CACGCGCATC
GGCGGTGAGG
TTGCCGAGGC
GCTGGCCGGG
TACGAGCTGC
CCATTCTTGA
GTCCCGTATC
ACGCAGCGCG
TGAGCTACCC
AGGCACTGCC
GCCGCCGGCA
CAACCGTTCT
TGAATCAGAA
CCCGAGGGCG
ACGCTGCCCG
CGAGGTCCAG
GCGCTGGCCG
CTGAAATTAA
ATCAAAACTC
ATTTGAGTTA
ATGAGGTAAA
GAGAAAATGA
GCAAAAGCAC
AAACACGCTA
AGTGCCGGCC
GTCCGAGCGC
ACGCAGCAGC
AAGGCTGCAA
CGTTGGCCAG
CCTGGCAGAC
ACGCCAGCCA
TGAAGCGGGT
CAACTTTCAG
TTGCCGGCGG
AGGATCACAC
CAAGCTGAAG
ATGTACGCGG
TACGCCAAGG
CAAGACCATT
ACCGAGCTGC
TATCTGAATA
CATCGCGCAG
CTACCAGAGT
AAATGAGCAA
ATGAATAAAT
GAGTAGATGA
ATTTTAGCGG
CTAAAGGAGG
CGGCATGGAA
AATCAAGAAC
AACCAGGCAC
CGACGCCGTG
GAATGCCCCA
TGTGTGGAGG
AACGGGCGGT
TGGCCAGGCG
TAAGCGGCTG
GGTTGCCTGC
CGGCCCTGCA
ATGGCACTGG
AACCCCCAAG
CCCGAGGAAT
CGGCGTGAGC
GGTCGCAAAC
CATCCGGCCC
GGTACAAATC
GGCGCGGCGC
TGGGTGATGA
CCTGGTGGAG
AAGTTGAAGG
CCGCGCAGGC
CGCCCAGCGG
CAACGCATCG
AGGCAGAAGC
ACGCCCCGGT
GAATCGTGGC
AAGCGGCCGC
TGATCGAATC
CGCAAAGAAT
CCCGGCAACC
GCCGGCAGCC
GGTGCGCCGT
CGATTAGGAA
GCCGCCCAAG
GGCGACGAGC
AACCAGATTT
TTTCGTTCCG
ATGCTCTATG
ACGTGGGCAC
CCGCGATAGT
CGCAGCATCA
TGGACGTGGC
CGTTTTCCGT
CTGTCGAAGC
GTGACCGACG
AGCTGGCGAG
GTGATCCGCT
ACGAGCTTCC
AGACGGGCAC
GTAGAGGTTT
CCGCAGGGCC
GGCCGGCATG
GCCAGTGTGT
GGGATTACGA
CCTGGTACTG
ATGGCGGTTT
CCCATCTAAC
CGAATCCATG
AACCGATACC
GGGAAGGGAA
GGGAGACAAG
CCCGGCCGCG
TGTTCCGTCC
ACACGTTGCG
GACGTACTCA
AGTTCTGCCG
GCGAGCCGAT
GGCGGAAAGC
AGAAAGACGA
CCTGGTAGAA
ACCTGCATTC
GGTTAAACAC
CACGCACGTT
GCCATGCAGC
GTACGAAGAA
GGCCAAGAAC
GGCCGCCTGG
TGACGGTATC
CGAGGGTGAA
GCCTTGATTA
GCCGCTACAA
GATCGTAAAG
AGCGAAACCG
GGCGGCCGGA
GTACATCGAG
ATCGAGCTAG
CTGATTGGAT
GTACCGCGAG
ATCACAGAAG
GCAAGAACCC
GGACGTGCTG
ACGGTTCACC
CCGATTACTT
TTTGATCGAT
CCCGGCATCG
GCCGTTTTCT
CTACCGCCTG
GCACGCCGCG
CCGCAGGCAA
GGCAGAAGCC
AGATGGTTGT
TCAAGACGAT
CTACGAACGC
AGTGGCAGCG
CCGGAGAGTT
CAAGAAGTTC
TGTTTCACCG
TGCGCAAGCT
GATCGGGTCA
AATGACCTGC
CGGAGTACGA
TTTGAAGGAG
GAGGCGGGGC
AGGCTGGCCC
GATCCTAGTC
ATGCGCTACC
GCAACCTGAT
CGAGGGCGAA
GCATCCGCCG
GTTCCTAATG
TACGGAGCAG
ATGCTAGGGC
AAATTGCCCT
AGCAGGGGAA
AAAGGTCGAA
AAGGTCTCTT
TCCTGTGGAT
AGCACGTACA
TTGGGAACCC
AAAGCCGTAC
ATTGGGAACC
GGAACCCGTA
CATTGGGAAC
CCAAAGCCGT
ACATTGGGAA
CCGGTCACAC
ATGTAAGTGA
CTGATATAAA
AGAGAAAAAA
GGCGATTTTT
CCGCCTAAAA
CTCTTTAAAA
CTTATTAAAA
CTCTTAAAAC
CCGCCTGGCC
TGTGCATAAC
TGTCTGGCCA
GCGCACAGCC
GAAGAGCTGC
AAAAAGCGCC
TACCCTTCGG
TCGCTGCGCT
CCCTACGCCC
CGCCGCTTCG
CGTCGGCCTA
TCGCGGCCGC
TGGCCGCTCA
AAAATGGCTG
GCCTACGGCC
AGGCAATCTA
CCAGGGCGCG
GACAAGCCGC
GCCGTCGCCA
CTCGACCGCC
GGCGCCCACA
TCAAGGCACC
CTGCCTCGCG
CGTTTCGGTG
ATGACGGTGA
AAACCTCTGA
CACATGCAGC
TCCCGGAGAC
GGTCACAGCT
TGTCTGTAAG
CGGATGCCGG
GAGCAGACAA
GCCCGTCAGG
GCGCGTCAGC
GGGTGTTGGC
GGGTGTCGGG
GCGCAGCCAT
GACCCAGTCA
CGTAGCGATA
GCGGAGTGTA
TACTGGCTTA
ACTATGCGGC
ATCAGAGCAG
ATTGTACTGA
GAGTGCACCA
TATGCGGTGT
GAAATACCGC
ACAGATGCGT
AAGGAGAAAA
TACCGCATCA
GGCGCTCTTC
CGCTTCCTCG
CTCACTGACT
CGCTGCGCTC
GGTCGTTCGG
CTGCGGCGAG
CGGTATCAGC
TCACTCAAAG
GCGGTAATAC
GGTTATCCAC
AGAATCAGGG
GATAACGCAG
GAAAGAACAT
GTGAGCAAAA
GGCCAGCAAA
AGGCCAGGAA
CCGTAAAAAG
GCCGCGTTGC
TGGCGTTTTT
CCATAGGCTC
CGCCCCCCTG
ACGAGCATCA
CAAAAATCGA
CGCTCAAGTC
AGAGGTGGCG
AAACCCGACA
GGACTATAAA
GATACCAGGC
GTTTCCCCCT
GGAAGCTCCC
TCGTGCGCTC
TCCTGTTCCG
ACCCTGCCGC
TTACCGGATA
CCTGTCCGCC
TTTCTCCCTT
CGGGAAGCGT
GGCGCTTTCT
CATAGCTCAC
GCTGTAGGTA
TCTCAGTTCG
GTGTAGGTCG
TTCGCTCCAA
GCTGGGCTGT
GTGCACGAAC
CCCCCGTTCA
GCCCGACCGC
TGCGCCTTAT
CCGGTAACTA
TCGTCTTGAG
TCCAACCCGG
TAAGACACGA
CTTATCGCCA
CTGGCAGCAG
CCACTGGTAA
CAGGATTAGC
AGAGCGAGGT
ATGTAGGCGG
TGCTACAGAG
TTCTTGAAGT
GGTGGCCTAA
CTACGGCTAC
ACTAGAAGGA
CAGTATTTGG
TATCTGCGCT
CTGCTGAAGC
CAGTTACCTT
CGGAAAAAGA
GTTGGTAGCT
CTTGATCCGG
CAAACAAACC
ACCGCTGGTA
GCGGTGGTTT
TTTTGTTTGC
AAGCAGCAGA
TTACGCGCAG
AAAAAAAGGA
TCTCAAGAAG
ATCCTTTGAT
CTTTTCTACG
GGGTCTGACG
CTCAGTGGAA
CGAAAACTCA
CGTTAAGGGA
TTTTGGTCAT
GCATTCTAGG
TACTAAAACA
ATTCATCCAG
TAAAATATAA
TATTTTATTT
TCTCCCAATC
AGGCTTGATC
CCCAGTAAGT
CAAAAAATAG
CTCGACATAC
TGTTCTTCCC
CGATATCCTC
CCTGATCGAC
CGGACGCAGA
AGGCAATGTC
ATACCACTTG
TCCGCCCTGC
CGCTTCTCCC
AAGATCAATA
AAGCCACTTA
CTTTGCCATC
TTTCACAAAG
ATGTTGCTGT
CTCCCAGGTC
GCCGTGGGAA
AAGACAAGTT
CCTCTTCGGG
CTTTTCCGTC
TTTAAAAAAT
CATACAGCTC
GCGCGGATCT
TTAAATGGAG
TGTCTTCTTC
CCAGTTTTCG
CAATCCACAT
CGGCCAGATC
GTTATTCAGT
AAGTAATCCA
ATTCGGCTAA
GCGGCTGTCT
AAGCTATTCG
TATAGGGACA
ATCCGATATG
TCGATGGAGT
GAAAGAGCCT
GATGCACTCC
GCATACAGCT
CGATAATCTT
TTCAGGGCTT
TGTTCATCTT
CATACTCTTC
CGAGCAAAGG
ACGCCATCGG
CCTCACTCAT
GAGCAGATTG
CTCCAGCCAT
CATGCCGTTC
AAAGTGCAGG
ACCTTTGGAA
CAGGCAGCTT
TCCTTCCAGC
CATAGCATCA
TGTCCTTTTC
CCGTTCCACA
TCATAGGTGG
TCCCTTTATA
CCGGCTGTCC
GTCATTTTTA
AATATAGGTT
TTCATTTTCT
CCCACCAGCT
TATATACCTT
AGCAGGAGAC
ATTCCTTCCG
TATCTTTTAC
GCAGCGGTAT
TTTTCGATCA
GTTTTTTCAA
TTCCGGTGAT
ATTCTCATTT
TAGCCATTTA
TTATTTCCTT
CCTCTTTTCT
ACAGTATTTA
AAGATACCCC
AAGAAGCTAA
TTATAACAAG
ACGAACTCCA
ATTCACTGTT
CCTTGCATTC
TAAAACCTTA
AATACCAGAA
AACAGCTTTT
TCAAAGTTGT
TTTCAAAGTT
GGCGTATAAC
ATAGTATCGA
CGGAGCCGAT
TTTGAAACCG
CGGTGATCAC
AGGCAGCAAC
GCTCTGTCAT
CGTTACAATC
AACATGCTAC
CCTCCGCGAG
ATCATCCGTG
TTTCAAACCC
GGCAGCTTAG
TTGCCGTTCT
TCCGAATAGC
ATCGGTAACA
TGAGCAAAGT
CTGCCGCCTT
ACAACGGCTC
TCCCGCTGAC
GCCGTCCCGG
ACTGATGGGC
TGCCTGTATC
GAGTGGTGAT
TTTGTGCCGA
GCTGCCGGTC
GGGGAGCTGT
TGGCTGG