名称: | pGAG |
---|---|
别称: | - |
基因长: | 1500bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 病毒 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | CAG |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | psPAX2 |
正向引物: | pcaggs-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TTGATTATTG
ACTAGTTATT
AATAGTAATC
AATTACGGGG
TCATTAGTTC
ATAGCCCATA
TATGGAGTTC
CGCGTTACAT
AACTTACGGT
AAATGGCCCG
CCTGGCTGAC
CGCCCAACGA
CCCCCGCCCA
TTGACGTCAA
TAATGACGTA
TGTTCCCATA
GTAACGCCAA
TAGGGACTTT
CCATTGACGT
CAATGGGTGG
AGTATTTACG
GTAAACTGCC
CACTTGGCAG
TACATCAAGT
GTATCATATG
CCAAGTACGC
CCCCTATTGA
CGTCAATGAC
GGTAAATGGC
CCGCCTGGCA
TTATGCCCAG
TACATGACCT
TATGGGACTT
TCCTACTTGG
CAGTACATCT
ACGTATTAGT
CATCGCTATT
ACCATGGTCG
AGGTGAGCCC
CACGTTCTGC
TTCACTCTCC
CCATCTCCCC
CCCCTCCCCA
CCCCCAATTT
TGTATTTATT
TATTTTTTAA
TTATTTTGTG
CAGCGATGGG
GGCGGGGGGG
GGGGGGGGGC
GCGCCrGGGG
GGGGGGGGGG
GGGGGGGGGG
GGGGGGGGGG
sGrGGsGGrG
rGGkGCGGCG
GCAGCCAATC
AGAGCGGCGC
GCTCCGAAAG
TTTCCTTTTA
TGGCGAGGCG
GCGGCGGCGG
CGGCCCTATA
AAAAGCGAAG
CGCGCGGCGG
GCGGGAGTCG
CTGCGCGCTG
CCTTCGCCCC
GTGCCCCGCT
CCGCCGCCGC
CTCGCGCCGC
CCGCCCCGGC
TCTGACTGAC
CGCGTTACTC
CCACAGGTGA
GCGGGCGGGA
CGGCCCTTCT
CCTCCGGGCT
GTAATTAGCG
CTTGGTTTAA
TGACGGCTTG
TTTCTTTTCT
GTGGCTGCGT
GAAAGCCTTG
AGGGGCTCCG
GGAGGGCCCT
TTGTGCGGGG
GGAGCGGCTC
GGGGGGTGCG
TGCGTGTGTG
TGTGCGTGGG
GAGCGCCGCG
TGCGGCTCCG
CGCTGCCCGG
CGGCTGTGAG
CGCTGCGGGC
GCGGCGCGGG
GCTTTGTGCG
CTCCGCAGTG
TGCGCGAGGG
GAGCGCGGCC
GGGGGCGGTG
CCCCGCGGTG
CGGGGGGGGC
TGCGAGGGGA
ACAAAGGCTG
CGTGCGGGGT
GTGTGCGTGG
GGGGGTGAGC
AGGGGGTGTG
GGCGCGTCGG
TCGGGCTGCA
ACCCCCCCTG
CACCCCCCTC
CCCGAGTTGC
TGAGCACGGC
CCGGCTTCGG
GTGCGGGGCT
CCGTACGGGG
CGTGGCGCGG
GGCTCGCCGT
GCCGGGCGGG
GGGTGGCGGC
AGGTGGGGGT
GCCGGGCGGG
GCGGGGCCGC
CyCGGGCCGG
GGrGGGCTCG
GGGGrGGGGC
GCGGCGGCCC
CCGGAGCGCC
GGCGGCTGTC
GAGGCGCGGC
GAGCCGCAGC
CATTGCCTTT
TATGGTAATC
GTGCGAGAGG
GCGCAGGGAC
TTCCTTTGTC
CCAAATCTGT
GCGGAGCCGA
AATCTGGGAG
GCGCCGCCGC
ACCCCCTCTA
GCGGGCGCGG
GGCGAAGCGG
TGCGGCGCCG
GCAGGAAGGA
AATGGGCGGG
GAGGGCCTTC
GTGCGTCGCC
GCGCCGCCGT
CCCCTTCTCC
CTCTCCAGCC
TCGGGGCTGT
CCGCGGGGGG
ACGGCTGCCT
TCGGGGGGGA
CGGGGCAGGG
CGGGGTTCGG
CTTCTGGCGT
GTGACCGGCG
GCTCTAGAGC
CTCTGCTAAC
CATGTTCATG
CCTTCTTCTT
TTTCCTACAG
CTCCTGGGCA
ACGTGCTGGT
TATTGTGCTG
TCTCATCATT
TTGGCAAAGA
ATTCGGGCCG
CGTTGACGCG
CACGGCAAGA
GGCGAGGGGC
GGCGACTGGT
GAGAGATGGG
TGCGAGAGCG
TCAGTATTAA
GCGGGGGAGA
ATTAGATCGA
TGGGAAAAAA
TTCGGTTAAG
GCCAGGGGGA
AAGAAAAAAT
ATAAATTAAA
ACATATAGTA
TGGGCAAGCA
GGGAGCTAGA
ACGATTCGCA
GTTAATCCTG
GCCTGTTAGA
AACATCAGAA
GGCTGTAGAC
AAATACTGGG
ACAGCTACAA
CCATCCCTTC
AGACAGGATC
AGAAGAACTT
AGATCATTAT
ATAATACAGT
AGCAACCCTC
TATTGTGTGC
ATCAAAGGAT
AGAGATAAAA
GACACCAAGG
AAGCTTTAGA
CAAGATAGAG
GAAGAGCAAA
ACAAAAGTAA
GAAAAAAGCA
CAGCAAGCAG
CAGCTGACAC
AGGACACAGC
AATCAGGTCA
GCCAAAATTA
CCCTATAGTG
CAGAACATCC
AGGGGCAAAT
GGTACATCAG
GCCATATCAC
CTAGAACTTT
AAATGCATGG
GTAAAAGTAG
TAGAAGAGAA
GGCTTTCAGC
CCAGAAGTGA
TACCCATGTT
TTCAGCATTA
TCAGAAGGAG
CCACCCCACA
AGATTTAAAC
ACCATGCTAA
ACACAGTGGG
GGGACATCAA
GCAGCCATGC
AAATGTTAAA
AGAGACCATC
AATGAGGAAG
CTGCAGAATG
GGATAGAGTG
CATCCAGTGC
ATGCAGGGCC
TATTGCACCA
GGCCAGATGA
GAGAACCAAG
GGGAAGTGAC
ATAGCAGGAA
CTACTAGTAC
CCTTCAGGAA
CAAATAGGAT
GGATGACACA
TAATCCACCT
ATCCCAGTAG
GAGAAATCTA
TAAAAGATGG
ATAATCCTGG
GATTAAATAA
AATAGTAAGA
ATGTATAGCC
CTACCAGCAT
TCTGGACATA
AGACAAGGAC
CAAAGGAACC
CTTTAGAGAC
TATGTAGACC
GATTCTATAA
AACTCTAAGA
GCCGAGCAAG
CTTCACAAGA
GGTAAAAAAT
TGGATGACAG
AAACCTTGTT
GGTCCAAAAT
GCGAACCCAG
ATTGTAAGAC
TATTTTAAAA
GCATTGGGAC
CAGGAGCGAC
ACTAGAAGAA
ATGATGACAG
CATGTCAGGG
AGTGGGGGGA
CCCGGCCATA
AAGCAAGAGT
TTTGGCTGAA
GCAATGAGCC
AAGTAACAAA
TCCAGCTACC
ATAATGATAC
AGAAAGGCAA
TTTTAGGAAC
CAAAGAAAGA
CTGTTAAGTG
TTTCAATTGT
GGCAAAGAAG
GGCACATAGC
CAAAAATTGC
AGGGCCCCTA
GGAAAAAGGG
CTGTTGGAAA
TGTGGAAAGG
AAGGACACCA
AATGAAAGAT
TGTACTGAGA
GACAGGCTAA
TTTTTTAGGG
AAGATCTGGC
CTTCCCACAA
GGGAAGGCCA
GGGAATTTTC
TTCAGAGCAG
ACCAGAGCCA
ACAGCCCCAC
CAGAAGAGAG
CTTCAGGTTT
GGGGAAGAGA
CAACAACTCC
CTCTCAGAAG
CAGGAGCCGA
TAGACAAGGA
ACTGTATCCT
TTAGCTTCCC
TCAGATCACT
CTTTGGCAGC
GACCCCTCGT
CACAACTCCC
CCCTCTGGAG
CGCCTTACTC
TTGGCTCCCT
GCAGTTGCCC
CCACTGGAGC
GGCTGACCCT
GGGGTCATTG
CAGTTGCCTC
CACTCGAACG
ACTGACGTTG
AGCCAGAACT
ATCCGATTGT
GCAGACCGGT
ATGACGCGTG
CGGCCGCGCT
AGCAGATCTT
TTTCCCTCTG
CCAAAAATTA
TGGGGACATC
ATGAAGCCCC
TTGAGCATCT
GACTTCTGGC
TAATAAAGGA
AATTTATTTT
CATTGCAATA
GTGTGTTGGA
ATTTTTTGTG
TCTCTCACTC
GGAAGGACAT
ATGGGAGGGC
AAATCATTTA
AAACATCAGA
ATGAGTATTT
GGTTTAGAGT
TTGGCAACAT
ATGCCCATAT
GCTGGCTGCC
ATGAACAAAG
GTTGGCTATA
AAGAGGTCAT
CAGTATATGA
AACAGCCCCC
TGCTGTCCAT
TCCTTATTCC
ATAGAAAAGC
CTTGACTTGA
GGTTAGATTT
TTTTTATATT
TTGTTTTGTG
TTATTTTTTT
CTTTAACATC
CCTAAAATTT
TCCTTACATG
TTTTACTAGC
CAGATTTTTC
CTCCTCTCCT
GACTACTCCC
AGTCATAGCT
GTCCCTCTTC
TCTTATGGAG
ATCCCTCGAC
CTGCAGCCCA
AGCTTGGCGT
AATCATGGTC
ATAGCTGTTT
CCTGTGTGAA
ATTGTTATCC
GCTCACAATT
CCACACAACA
TACGAGCCGG
AAGCATAAAG
TGTAAAGCCT
GGGGTGCCTA
ATGAGTGAGC
TAACTCACAT
TAATTGCGTT
GCGCTCACTG
CCCGCTTTCC
AGTCGGGAAA
CCTGTCGTGC
CAGCGGATCC
GCATCTCAAT
TAGTCAGCAA
CCATAGTCCC
GCCCCTAACT
CCGCCCATCC
CGCCCCTAAC
TCCGCCCAGT
TCCGCCCATT
CTCCGCCCCA
TGGCTGACTA
ATTTTTTTTA
TTTATGCAGA
GGCCGAGGCC
GCCTCGGCCT
CTGAGCTATT
CCAGAAGTAG
TGAGGAGGCT
TTTTTGGAGG
CCTAGGCTTT
TGCAAAAAGC
TAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTATCATGTC
TGGATCCGCT
GCATTAATGA
ATCGGCCAAC
GCGCGGGGAG
AGGCGGTTTG
CGTATTGGGC
GCTCTTCCGC
TTCCTCGCTC
ACTGACTCGC
TGCGCTCGGT
CGTTCGGCTG
CGGCGAGCGG
TATCAGCTCA
CTCAAAGGCG
GTAATACGGT
TATCCACAGA
ATCAGGGGAT
AACGCAGGAA
AGAACATGTG
AGCAAAAGGC
CAGCAAAAGG
CCAGGAACCG
TAAAAAGGCC
GCGTTGCTGG
CGTTTTTCCA
TAGGCTCCGC
CCCCCTGACG
AGCATCACAA
AAATCGACGC
TCAAGTCAGA
GGTGGCGAAA
CCCGACAGGA
CTATAAAGAT
ACCAGGCGTT
TCCCCCTGGA
AGCTCCCTCG
TGCGCTCTCC
TGTTCCGACC
CTGCCGCTTA
CCGGATACCT
GTCCGCCTTT
CTCCCTTCGG
GAAGCGTGGC
GCTTTCTCAT
AGCTCACGCT
GTAGGTATCT
CAGTTCGGTG
TAGGTCGTTC
GCTCCAAGCT
GGGCTGTGTG
CACGAACCCC
CCGTTCAGCC
CGACCGCTGC
GCCTTATCCG
GTAACTATCG
TCTTGAGTCC
AACCCGGTAA
GACACGACTT
ATCGCCACTG
GCAGCAGCCA
CTGGTAACAG
GATTAGCAGA
GCGAGGTATG
TAGGCGGTGC
TACAGAGTTC
TTGAAGTGGT
GGCCTAACTA
CGGCTACACT
AGAAGAACAG
TATTTGGTAT
CTGCGCTCTG
CTGAAGCCAG
TTACCTTCGG
AAAAAGAGTT
GGTAGCTCTT
GATCCGGCAA
ACAAACCACC
GCTGGTAGCG
GTGGTTTTTT
TGTTTGCAAG
CAGCAGATTA
CGCGCAGAAA
AAAAGGATCT
CAAGAAGATC
CTTTGATCTT
TTCTACGGGG
TCTGACGCTC
AGTGGAACGA
AAACTCACGT
TAAGGGATTT
TGGTCATGAG
ATTATCAAAA
AGGATCTTCA
CCTAGATCCT
TTTAAATTAA
AAATGAAGTT
TTAAATCAAT
CTAAAGTATA
TATGAGTAAA
CTTGGTCTGA
CAGTTACCAA
TGCTTAATCA
GTGAGGCACC
TATCTCAGCG
ATCTGTCTAT
TTCGTTCATC
CATAGTTGCC
TGACTCCCCG
TCGTGTAGAT
AACTACGATA
CGGGAGGGCT
TACCATCTGG
CCCCAGTGCT
GCAATGATAC
CGCGAGACCC
ACGCTCACCG
GCTCCAGATT
TATCAGCAAT
AAACCAGCCA
GCCGGAAGGG
CCGAGCGCAG
AAGTGGTCCT
GCAACTTTAT
CCGCCTCCAT
CCAGTCTATT
AATTGTTGCC
GGGAAGCTAG
AGTAAGTAGT
TCGCCAGTTA
ATAGTTTGCG
CAACGTTGTT
GCCATTGCTA
CAGGCATCGT
GGTGTCACGC
TCGTCGTTTG
GTATGGCTTC
ATTCAGCTCC
GGTTCCCAAC
GATCAAGGCG
AGTTACATGA
TCCCCCATGT
TGTGCAAAAA
AGCGGTTAGC
TCCTTCGGTC
CTCCGATCGT
TGTCAGAAGT
AAGTTGGCCG
CAGTGTTATC
ACTCATGGTT
ATGGCAGCAC
TGCATAATTC
TCTTACTGTC
ATGCCATCCG
TAAGATGCTT
TTCTGTGACT
GGTGAGTACT
CAACCAAGTC
ATTCTGAGAA
TAGTGTATGC
GGCGACCGAG
TTGCTCTTGC
CCGGCGTCAA
TACGGGATAA
TACCGCGCCA
CATAGCAGAA
CTTTAAAAGT
GCTCATCATT
GGAAAACGTT
CTTCGGGGCG
AAAACTCTCA
AGGATCTTAC
CGCTGTTGAG
ATCCAGTTCG
ATGTAACCCA
CTCGTGCACC
CAACTGATCT
TCAGCATCTT
TTACTTTCAC
CAGCGTTTCT
GGGTGAGCAA
AAACAGGAAG
GCAAAATGCC
GCAAAAAAGG
GAATAAGGGC
GACACGGAAA
TGTTGAATAC
TCATACTCTT
CCTTTTTCAA
TATTATTGAA
GCATTTATCA
GGGTTATTGT
CTCATGAGCG
GATACATATT
TGAATGTATT
TAGAAAAATA
AACAAATAGG
GGTTCCGCGC
ACATTTCCCC
GAAAAGTGCC
ACCTGGGTCG
ACA