名称: | pCas9(Plasmid#162606) |
---|---|
别称: | Plasmid#162606 |
基因长: | |
质粒宿主: | 酵母菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR,Cas9 |
片段物种: | |
原核抗性: | Amp |
筛选标记: | LUE2, Hyg |
荧光标记: | |
启动子: | |
复制子: | p15A |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | T3 |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GACATGGAGG
CCCAGAATAC
CCTCCTTGAC
AGTCTTGACG
TGCGCAGCTC
AGGGGCATGA
TGTGACTGTC
GCCCGTACAT
TTAGCCCATA
CATCCCCATG
TATAATCATT
TGCATCCATA
CATTTTGATG
GCCGCACGGC
GCGAAGCAAA
AATTACGGCT
CCTCGCTGCG
GACCTGCGAG
CAGGGAAACG
CTCCCCTCAC
AGACGCGTTG
AATTGTCCCC
ACGCCGCGCC
CCTGTAGAGA
AATATAAAAG
GTTAGGATTT
GCCACTGAGG
TTCTTCTTTC
ATATACTTCC
TTTTAAAATC
TTGCTAGGAT
ACAGTTCTCA
CATCACATCC
GAACATAAAC
AACCATGGGT
AAAAAGCCTG
AACTCACCGC
GACGTCTGTC
GAGAAGTTTC
TGATCGAAAA
GTTCGACAGC
GTCTCCGACC
TGATGCAGCT
CTCGGAGGGC
GAAGAATCTC
GTGCTTTCAG
CTTCGATGTA
GGAGGGCGTG
GATATGTCCT
GCGGGTAAAT
AGCTGCGCCG
ATGGTTTCTA
CAAAGATCGT
TATGTTTATC
GGCACTTTGC
ATCGGCCGCG
CTCCCGATTC
CGGAAGTGCT
TGACATTGGG
GAATTCAGCG
AGAGCCTGAC
CTATTGCATC
TCCCGCCGTG
CACAGGGTGT
CACGTTGCAA
GACCTGCCTG
AAACCGAACT
GCCCGCTGTT
CTGCAGCCGG
TCGCGGAGGC
CATGGATGCG
ATCGCTGCGG
CCGATCTTAG
CCAGACGAGC
GGGTTCGGCC
CATTCGGACC
GCAAGGAATC
GGTCAATACA
CTACATGGCG
TGATTTCATA
TGCGCGATTG
CTGATCCCCA
TGTGTATCAC
TGGCAAACTG
TGATGGACGA
CACCGTCAGT
GCGTCCGTCG
CGCAGGCTCT
CGATGAGCTG
ATGCTTTGGG
CCGAGGACTG
CCCCGAAGTC
CGGCACCTCG
TGCACGCGGA
TTTCGGCTCC
AACAATGTCC
TGACGGACAA
TGGCCGCATA
ACAGCGGTCA
TTGACTGGAG
CGAGGCGATG
TTCGGGGATT
CCCAATACGA
GGTCGCCAAC
ATCTTCTTCT
GGAGGCCGTG
GTTGGCTTGT
ATGGAGCAGC
AGACGCGCTA
CTTCGAGCGG
AGGCATCCGG
AGCTTGCAGG
ATCGCCGCGG
CTCCGGGCGT
ATATGCTCCG
CATTGGTCTT
GACCAACTCT
ATCAGAGCTT
GGTTGACGGC
AATTTCGATG
ATGCAGCTTG
GGCGCAGGGT
CGATGCGACG
CAATCGTCCG
ATCCGGAGCC
GGGACTGTCG
GGCGTACACA
AATCGCCCGC
AGAAGCGCGG
CCGTCTGGAC
CGATGGCTGT
GTAGAAGTAC
TCGCCGATAG
TGGAAACCGA
CGCCCCAGCA
CTCGTCCGAG
GGCAAAGGAA
TAATCAGTAC
TGACAATAAA
AAGATTCTTG
TTTTCAAGAA
CTTGTCATTT
GTATAGTTTT
TTTATATTGT
AGTTGTTCTA
TTTTAATCAA
ATGTTAGCGT
GATTTATATT
TTTTTTCGCC
TCGACATCAT
CTGCCCAGAT
GCGAAGTTAA
GTGCGCAGAA
AGTAATATCA
TGCGTCAATC
GTATGTGAAT
GCTGGTCGCT
ATACTGCTGT
CGATTCGATA
CTAACGCCGC
CATCCAGTGT
TAATTAACGA
CGGTCGAGGA
GAACTTCTAG
TATATCTACA
TACCTAATAT
TATTGCCTTA
TTAAAAATGG
AATCCCAACA
ATTACATCAA
AATCCACATT
CTCTTCAAAA
TCAATTGTCC
TGTACTTCCT
TGTTCATGTG
TGTTCAAAAA
CGTTATATTT
ATAGGATAAT
TATACTCTAT
TTCTCAACAA
GTAATTGGTT
GTTTGGCCGA
GCGGTCTAAG
GCGCCTGATT
CAAGAAATAT
CTTGACCGCA
GTTAACTGTG
GGAATACTCA
GGTATCGTAA
GATGCAAGAG
TTCGAATCTC
TTAGCAACCA
TTATTTTTTT
CCTCAACATA
ACGAGAACAC
ACAGGGGCGC
TATCGCACAG
AATCAAATTC
GATGACTGGA
AATTTTTTGT
TAATTTCAGA
GGTCGCCTGA
CGCATATACC
TTTTTCAACT
GAAAAATTGG
GAGAAAAAGG
AAAGGTGAGA
GCGCCGGAAC
CGGCTTTTCA
TATAGAATAG
AGAAGCGTTC
ATGACTAAAT
GCTTGCATCA
CAATACTTGA
AGTTGACAAT
ATTATTTAAG
GACCTATTGT
TTTTTCCAAT
AGGTGGTTAG
CAATCGTCTT
ACTTTCTAAC
TTTTCTTACC
TTTTACATTT
CAGCAATATA
TATATATATA
TTTCAAGGAT
ATACCATTCT
AATGTCTGCC
CCTAAGAAGA
TCGTCGTTTT
GCCAGGTGAC
CACGTTGGTC
AAGAAATCAC
AGCCGAAGCC
ATTAAGGTTC
TTAAAGCTAT
TTCTGATGTT
CGTTCCAATG
TCAAGTTCGA
TTTCGAAAAT
CATTTAATTG
GTGGTGCTGC
TATCGATGCT
ACAGGTGTTC
CACTTCCAGA
TGAGGCGCTG
GAAGCCTCCA
AGAAGGCTGA
TGCCGTTTTG
TTAGGTGCTG
TGGGTGGTCC
TAAATGGGGT
ACCGGTAGTG
TTAGACCTGA
ACAAGGTTTA
CTAAAAATCC
GTAAAGAACT
TCAATTGTAC
GCCAACTTAA
GACCATGTAA
CTTTGCATCC
GACTCTCTTT
TAGACTTATC
TCCAATCAAG
CCACAATTTG
CTAAAGGTAC
TGACTTCGTT
GTTGTCAGAG
AATTAGTGGG
AGGTATTTAC
TTTGGTAAGA
GAAAGGAAGA
CGATGGTGAT
GGTGTCGCTT
GGGATAGTGA
ACAATACACC
GTTCCAGAAG
TGCAAAGAAT
CACAAGAATG
GCCGCTTTCA
TGGCCCTACA
ACATGAGCCA
CCATTGCCTA
TTTGGTCCTT
GGATAAAGCT
AATGTTTTGG
CCTCTTCAAG
ATTATGGAGA
AAAACTGTGG
AGGAAACCAT
CAAGAACGAA
TTCCCTACAT
TGAAGGTTCA
ACATCAATTG
ATTGATTCTG
CCGCCATGAT
CCTAGTTAAG
AACCCAACCC
ACCTAAATGG
TATTATAATC
ACCAGCAATA
TGTTTGGTGA
TATCATCTCC
GATGAAGCCT
CCGTTATCCC
AGGTTCCTTG
GGTTTGTTGC
CATCTGCGTC
CTTGGCCTCT
TTGCCAGACA
AGAACACCGC
ATTTGGTTTG
TACGAACCAT
GCCACGGTTC
TGCTCCAGAT
TTGCCAAAGA
ATAAGGTCAA
CCCTATCGCC
ACTATCTTGT
CTGCTGCAAT
GATGTTGAAA
TTGTCATTGA
ACTTGCCTGA
AGAAGGTAAG
GCCATTGAAG
ATGCAGTTAA
AAAGGTTTTG
GATGCAGGTA
TCAGAACTGG
TGATTTAGGT
GGTTCCAACA
GTACCACCGA
AGTCGGTGAT
GCTGTCGCCG
AAGAAGTTAA
GAAAATCCTT
GCTTAAAAAG
ATTCTCTTTT
TTTATGATAT
TTGTACATAA
ACTTTATAAA
TGAAATTCAT
AATAGAAACG
ACACGAAATT
ACAAAATGGA
ATATGTTCAT
AGGGTAGACG
AAACTATATA
CGCAATCTAC
ATACATTTAT
CAAGAAGGAG
AAAAAGGAGG
ATGTAAAGGA
ATACAGGTAA
GCAAATTGAT
ACTAATGGCT
CAACGTGATA
AGGAAAAAGA
ATTGCACTTT
AACATTAATA
TTGACAAGGA
GGAGGGCACC
ACACAAAAAG
TTAGGTGTAA
CAGAAAATCA
TGAAACTATG
ATTCCTAATT
TATATATTGG
AGGATTTTCT
CTAAAAAAAA
AAAAATACAA
CAAATAAAAA
ACACTCAATG
ACCTGACCAT
TTGATGGAGT
TTAAGTCAAT
ACCTTCTTGA
ACCATTTCCC
ATAATGGTGA
AAGTTCCCTC
AAGAATTTTA
CTCTGTCAGA
AACGGCCTTA
ACGACGTAGT
CGATATGGTG
CACTCTCAGT
ACAATCTGCT
CTGATGCCGC
ATAGTTAAGC
CAGCCCCGAC
ACCCGCCAAC
ACCCGCTGAC
GCGCCCTGAC
GGGCTTGTCT
GCTCCCGGCA
TCCGCTTACA
GACAAGCTGT
GACCGTCTCC
GGGAGCTGCA
TGTGTCAGAG
GTTTTCACCG
TCATCACCGA
AACGCGCGAG
ACGAAAGGGC
CTCGTGATAC
GCCTATTTTT
ATAGGTTAAT
GTCATGATAA
TAATGGTTTC
TTAGGACGGA
TCGCTTGCCT
GTAACTTACA
CGCGCCTCGT
ATCTTTTAAT
GATGGAATAA
TTTGGGAATT
TACTCTGTGT
TTATTTATTT
TTATGTTTTG
TATTTGGATT
TTAGAAAGTA
AATAAAGAAG
GTAGAAGAGT
TACGGAATGA
AGAAAAAAAA
ATAAACAAAG
GTTTAAAAAA
TTTCAACAAA
AAGCGTACTT
TACATATATA
TTTATTAGAC
AAGAAAAGCA
GATTAAATAG
ATATACATTC
GATTAACGAT
AAGTAAAATG
TAAAATCACA
GGATTTTCGT
GTGTGGTCTT
CTACACAGAC
AAGATGAAAC
AATTCGGCAT
TAATACCTGA
GAGCAGGAAG
AAAAAGATAA
AAGGTAGTAT
TTGTTGGCGA
TCCCCCTAGA
GTCTTTTACA
TCTTCGGAAA
ACAAAAACTA
TTTTTTCTTT
AATTTCTTTT
TTTACTTTCT
ATTTTTAATT
TATATATTTA
TATTAAAAAA
TTTAAATTAT
AATTATTTTT
ATAGCACGTG
ATGAAAAGGA
CCCAGGTGGC
ACTTTTCGGG
GAAATGTGCG
CGGAACCCCT
ATTTGTTTAT
TTTTCTAAAT
ACATTCAAAT
ATGTATCCGC
TCATGAGACA
ATAACCCTGA
TAAATGCTTC
AATAATATTG
AAAAAGGAAG
AGTATGAGTA
TTCAACATTT
CCGTGTCGCC
CTTATTCCCT
TTTTTGCGGC
ATTTTGCCTT
CCTGTTTTTG
CTCACCCAGA
AACGCTGGTG
AAAGTAAAAG
ATGCTGAAGA
TCAGTTGGGT
GCACGAGTGG
GTTACATCGA
ACTGGATCTC
AACAGCGGTA
AGATCCTTGA
GAGTTTTCGC
CCCGAAGAAC
GTTTTCCAAT
GATGAGCACT
TTTAAAGTTC
TGCTATGTGG
CGCGGTATTA
TCCCGTATTG
ACGCCGGGCA
AGAGCAACTC
GGTCGCCGCA
TACACTATTC
TCAGAATGAC
TTGGTTGAGT
ACTCACCAGT
CACAGAAAAG
CATCTTACGG
ATGGCATGAC
AGTAAGAGAA
TTATGCAGTG
CTGCCATAAC
CATGAGTGAT
AACACTGCGG
CCAACTTACT
TCTGACAACG
ATCGGAGGAC
CGAAGGAGCT
AACCGCTTTT
TTGCACAACA
TGGGGGATCA
TGTAACTCGC
CTTGATCGTT
GGGAACCGGA
GCTGAATGAA
GCCATACCAA
ACGACGAGCG
TGACACCACG
ATGCCTGTAG
CAATGGCAAC
AACGTTGCGC
AAACTATTAA
CTGGCGAACT
ACTTACTCTA
GCTTCCCGGC
AACAATTAAT
AGACTGGATG
GAGGCGGATA
AAGTTGCAGG
ACCACTTCTG
CGCTCGGCCC
TTCCGGCTGG
CTGGTTTATT
GCTGATAAAT
CTGGAGCCGG
TGAGCGTGGG
TCTCGCGGTA
TCATTGCAGC
ACTGGGGCCA
GATGGTAAGC
CCTCCCGTAT
CGTAGTTATC
TACACGACGG
GGAGTCAGGC
AACTATGGAT
GAACGAAATA
GACAGATCGC
TGAGATAGGT
GCCTCACTGA
TTAAGCATTG
GTAACTGTCA
GACCAAGTTT
ACTCATATAT
ACTTTAGATT
GATTTAAAAC
TTCATTTTTA
ATTTAAAAGG
ATCTAGGTGA
AGATCCTTTT
TGATAATCTC
ATGACCAAAA
TCCCTTAACG
TGAGTTTTCG
TTCCACTGAG
CGTCAGACCC
CGTAGAAAAG
ATCAAAGGAT
CTTCTTGAGA
TCCTTTTTTT
CTGCGCGTAA
TCTGCTGCTT
GCAAACAAAA
AAACCACCGC
TACCAGCGGT
GGTTTGTTTG
CCGGATCAAG
AGCTACCAAC
TCTTTTTCCG
AAGGTAACTG
GCTTCAGCAG
AGCGCAGATA
CCAAATACTG
TTCTTCTAGT
GTAGCCGTAG
TTAGGCCACC
ACTTCAAGAA
CTCTGTAGCA
CCGCCTACAT
ACCTCGCTCT
GCTAATCCTG
TTACCAGTGG
CTGCTGCCAG
TGGCGATAAG
TCGTGTCTTA
CCGGGTTGGA
CTCAAGACGA
TAGTTACCGG
ATAAGGCGCA
GCGGTCGGGC
TGAACGGGGG
GTTCGTGCAC
ACAGCCCAGC
TTGGAGCGAA
CGACCTACAC
CGAACTGAGA
TACCTACAGC
GTGAGCTATG
AGAAAGCGCC
ACGCTTCCCG
AAGGGAGAAA
GGCGGACAGG
TATCCGGTAA
GCGGCAGGGT
CGGAACAGGA
GAGCGCACGA
GGGAGCTTCC
AGGGGGAAAC
GCCTGGTATC
TTTATAGTCC
TGTCGGGTTT
CGCCACCTCT
GACTTGAGCG
TCGATTTTTG
TGATGCTCGT
CAGGGGGGCG
GAGCCTATGG
AAAAACGCCA
GCAACGCGGC
CTTTTTACGG
TTCCTGGCCT
TTTGCTGGCC
TTTTGCTCAC
ATGTTCTTTC
CTGCGTTATC
CCCTGATTCT
GTGGATAACC
GTATTACCGC
CTTTGAGTGA
GCTGATACCG
CTCGCCGCAG
CCGAACGACC
GAGCGCAGCG
AGTCAGTGAG
CGAGGAAGCG
GAAGAGCGCC
CAATACGCAA
ACCGCCTCTC
CCCGCGCGTT
GGCCGATTCA
TTAATGCAGC
TGGCACGACA
GGTTTCCCGA
CTGGAAAGCG
GGCAGTGAGC
GCAACGCAAT
TAATGTGAGT
TAGCTCACTC
ATTAGGCACC
CCAGGCTTTA
CACTTTATGC
TTCCGGCTCG
TATGTTGTGT
GGAATTGTGA
GCGGATAACA
ATTTCACACA
GGAAACAGCT
ATGACCATGA
TTACGCCAAG
CGCGCAATTA
ACCCTCACTA
AAGGGAACAA
AAGCTGGAGC
TCATAGCTTC
AAAATGTTTC
TACTCCTTTT
TTACTCTTCC
AGATTTTCTC
GGACTCCGCG
CATCGCCGTA
CCACTTCAAA
ACACCCAAGC
ACAGCATACT
AAATTTCCCC
TCTTTCTTCC
TCTAGGGTGT
CGTTAATTAC
CCGTACTAAA
GGTTTGGAAA
AGAAAAAAGA
GACCGCCTCG
TTTCTTTTTC
TTCGTCGAAA
AAGGCAATAA
AAATTTTTAT
CACGTTTCTT
TTTCTTGAAA
ATTTTTTTTT
TGATTTTTTT
CTCTTTCGAT
GACCTCCCAT
TGATATTTAA
GTTAATAAAC
GGTCTTCAAT
TTCTCAAGTT
TCAGTTTCAT
TTTTCTTGTT
CTATTACAAC
TTTTTTTACT
TCTTGCTCAT
TAGAAAGAAA
GCATAGCAAT
CTAATCTAAG
TTTTCTAGAA
CTAGTGGATC
CCCCGGGAAA
AATGGACAAG
AAGTACTCCA
TTGGGCTCGA
TATCGGCACA
AACAGCGTCG
GTTGGGCCGT
CATTACGGAC
GAGTACAAGG
TGCCGAGCAA
AAAATTCAAA
GTTCTGGGCA
ATACCGATCG
CCACAGCATA
AAGAAGAACC
TCATTGGCGC
CCTCCTGTTC
GACTCCGGGG
AGACGGCCGA
AGCCACGCGG
CTCAAAAGAA
CAGCACGGCG
CAGATATACC
CGCAGAAAGA
ATCGGATCTG
CTACCTGCAG
GAGATCTTTA
GTAATGAGAT
GGCTAAGGTG
GATGACTCTT
TCTTCCATAG
GCTGGAGGAG
TCCTTTTTGG
TGGAGGAGGA
TAAAAAGCAC
GAGCGCCACC
CAATCTTTGG
CAATATCGTG
GACGAGGTGG
CGTACCATGA
AAAGTACCCA
ACCATATATC
ATCTGAGGAA
GAAGCTTGTA
GACAGTACTG
ATAAGGCTGA
CTTGCGGTTG
ATCTATCTCG
CGCTGGCGCA
TATGATCAAA
TTTCGGGGAC
ACTTCCTCAT
CGAGGGGGAC
CTGAACCCAG
ACAACAGCGA
TGTCGACAAA
CTCTTTATCC
AACTGGTTCA
GACTTACAAT
CAGCTTTTCG
AAGAGAACCC
GATCAACGCA
TCCGGAGTTG
ACGCCAAAGC
AATCCTGAGC
GCTAGGCTGT
CCAAATCCCG
GCGGCTCGAA
AACCTCATCG
CACAGCTCCC
TGGGGAGAAG
AAGAACGGCC
TGTTTGGTAA
TCTTATCGCC
CTGTCACTCG
GGCTGACCCC
CAACTTTAAA
TCTAACTTCG
ACCTGGCCGA
AGATGCCAAG
CTTCAACTGA
GCAAAGACAC
CTACGATGAT
GATCTCGACA
ATCTGCTGGC
CCAGATCGGC
GACCAGTACG
CAGACCTTTT
TTTGGCGGCA
AAGAACCTGT
CAGACGCCAT
TCTGCTGAGT
GATATTCTGC
GAGTGAACAC
GGAGATCACC
AAAGCTCCGC
TGAGCGCTAG
TATGATCAAG
CGCTATGATG
AGCACCACCA
AGACTTGACT
TTGCTGAAGG
CCCTTGTCAG
ACAGCAACTG
CCTGAGAAGT
ACAAGGAAAT
TTTCTTCGAT
CAGTCTAAAA
ATGGCTACGC
CGGATACATT
GACGGCGGAG
CAAGCCAGGA
GGAATTTTAC
AAATTTATTA
AGCCCATCTT
GGAAAAAATG
GACGGCACCG
AGGAGCTGCT
GGTAAAGCTT
AACAGAGAAG
ATCTGTTGCG
CAAACAGCGC
ACTTTCGACA
ATGGAAGCAT
CCCCCACCAG
ATTCACCTGG
GCGAACTGCA
CGCTATCCTC
AGGCGGCAAG
AGGATTTCTA
CCCCTTTTTG
AAAGATAACA
GGGAAAAGAT
TGAGAAAATC
CTCACATTTC
GGATACCCTA
CTATGTAGGC
CCCCTCGCCC
GGGGAAATTC
CAGATTCGCG
TGGATGACTC
GCAAATCAGA
AGAGACCATC
ACTCCCTGGA
ACTTCGAGGA
AGTCGTGGAT
AAGGGGGCCT
CTGCCCAGTC
CTTCATCGAA
AGGATGACTA
ACTTTGATAA
AAATCTGCCT
AACGAAAAGG
TGCTTCCTAA
ACACTCTCTG
CTGTACGAGT
ACTTCACAGT
TTATAACGAG
CTCACCAAGG
TCAAATACGT
CACAGAAGGG
ATGAGAAAGC
CAGCATTCCT
GTCTGGAGAG
CAGAAGAAAG
CTATCGTGGA
CCTCCTCTTC
AAGACGAACC
GGAAAGTTAC
CGTGAAACAG
CTCAAAGAAG
ACTATTTCAA
AAAGATTGAA
TGTTTCGACT
CTGTTGAAAT
CAGCGGAGTG
GAGGATCGCT
TCAACGCATC
CCTGGGAACG
TATCACGATC
TCCTGAAAAT
CATTAAAGAC
AAGGACTTCC
TGGACAATGA
GGAGAACGAG
GACATTCTTG
AGGACATTGT
CCTCACCCTT
ACGTTGTTTG
AAGATAGGGA
GATGATTGAA
GAACGCTTGA
AAACTTACGC
TCATCTCTTC
GACGACAAAG
TCATGAAACA
GCTCAAGAGG
CGCCGATATA
CAGGATGGGG
GCGGCTGTCA
AGAAAACTGA
TCAATGGGAT
CCGAGACAAG
CAGAGTGGAA
AGACAATCCT
GGATTTTCTT
AAGTCCGATG
GATTTGCCAA
CCGGAACTTC
ATGCAGTTGA
TCCATGATGA
CTCTCTCACC
TTTAAGGAGG
ACATCCAGAA
AGCACAAGTT
TCTGGCCAGG
GGGACAGTCT
TCACGAGCAC
ATCGCTAATC
TTGCAGGTAG
CCCAGCTATC
AAAAAGGGAA
TACTGCAGAC
CGTTAAGGTC
GTGGATGAAC
TCGTCAAAGT
AATGGGAAGG
CATAAGCCCG
AGAATATCGT
TATCGAGATG
GCCCGAGAGA
ACCAAACTAC
CCAGAAGGGA
CAGAAGAACA
GTAGGGAAAG
GATGAAGAGG
ATTGAAGAGG
GTATAAAAGA
ACTGGGGTCC
CAAATCCTTA
AGGAACACCC
AGTTGAAAAC
ACCCAGCTTC
AGAATGAGAA
GCTCTACCTG
TACTACCTGC
AGAACGGCAG
GGACATGTAC
GTGGATCAGG
AACTGGACAT
CAATCGGCTC
TCCGACTACG
ACGTGGATCA
TATCGTGCCC
CAGTCTTTTC
TCAAAGATGA
TTCTATTGAT
AATAAAGTGT
TGACAAGATC
CGATAAAAAT
AGAGGGAAGA
GTGATAACGT
CCCCTCAGAA
GAAGTTGTCA
AGAAAATGAA
AAATTATTGG
CGGCAGCTGC
TGAACGCCAA
ACTGATCACA
CAACGGAAGT
TCGATAATCT
GACTAAGGCT
GAACGAGGTG
GCCTGTCTGA
GTTGGATAAA
GCCGGCTTCA
TCAAAAGGCA
GCTTGTTGAG
ACACGCCAGA
TCACCAAGCA
CGTGGCCCAA
ATTCTCGATT
CACGCATGAA
CACCAAGTAC
GATGAAAATG
ACAAACTGAT
TCGAGAGGTG
AAAGTTATTA
CTCTGAAGTC
TAAGCTGGTC
TCAGATTTCA
GAAAGGACTT
TCAGTTTTAT
AAGGTGAGAG
AGATCAACAA
TTACCACCAT
GCGCATGATG
CCTACCTGAA
TGCAGTGGTA
GGCACTGCAC
TTATCAAAAA
ATATCCCAAG
CTTGAATCTG
AATTTGTTTA
CGGAGACTAT
AAAGTGTACG
ATGTTAGGAA
AATGATCGCA
AAGTCTGAGC
AGGAAATAGG
CAAGGCCACC
GCTAAGTACT
TCTTTTACAG
CAATATTATG
AATTTTTTCA
AGACCGAGAT
TACACTGGCC
AATGGAGAGA
TTCGGAAGCG
ACCACTTATC
GAAACAAACG
GAGAAACAGG
AGAAATCGTG
TGGGACAAGG
GTAGGGATTT
CGCGACAGTC
CGGAAGGTCC
TGTCCATGCC
GCAGGTGAAC
ATCGTTAAAA
AGACCGAAGT
ACAGACCGGA
GGCTTCTCCA
AGGAAAGTAT
CCTCCCGAAA
AGGAACAGCG
ACAAGCTGAT
CGCACGCAAA
AAAGATTGGG
ACCCCAAGAA
ATACGGCGGA
TTCGATTCTC
CTACAGTCGC
TTACAGTGTA
CTGGTTGTGG
CCAAAGTGGA
GAAAGGGAAG
TCTAAAAAAC
TCAAAAGCGT
CAAGGAACTG
CTGGGCATCA
CAATCATGGA
GCGATCAAGC
TTCGAAAAAA
ACCCCATCGA
CTTTCTCGAG
GCGAAAGGAT
ATAAAGAGGT
CAAAAAAGAC
CTCATCATTA
AGCTTCCCAA
GTACTCTCTC
TTTGAGCTTG
AAAACGGCCG
GAAACGAATG
CTCGCTAGTG
CGGGCGAGCT
GCAGAAAGGT
AACGAGCTGG
CACTGCCCTC
TAAATACGTT
AATTTCTTGT
ATCTGGCCAG
CCACTATGAA
AAGCTCAAAG
GGTCTCCCGA
AGATAATGAG
CAGAAGCAGC
TGTTCGTGGA
ACAACACAAA
CACTACCTTG
ATGAGATCAT
CGAGCAAATA
AGCGAATTCT
CCAAAAGAGT
GATCCTCGCC
GACGCTAACC
TCGATAAGGT
GCTTTCTGCT
TACAATAAGC
ACAGGGATAA
GCCCATCAGG
GAGCAGGCAG
AAAACATTAT
CCACTTGTTT
ACTCTGACCA
ACTTGGGCGC
GCCTGCAGCC
TTCAAGTACT
TCGACACCAC
CATAGACAGA
AAGCGGTACA
CCTCTACAAA
GGAGGTCCTG
GACGCCACAC
TGATTCATCA
GTCAATTACG
GGGCTCTATG
AAACAAGAAT
CGACCTCTCT
CAGCTCGGTG
GAGACAGCAG
GGCTGACCCC
AAGAAGAAGA
GGAAGGTGTG
ATCTCTTCTC
GAGTCATGTA
ATTAGTTATG
TCACGCTTAC
ATTCACGCCC
TCCCCCCACA
TCCGCTCTAA
CCGAAAAGGA
AGGAGTTAGA
CAACCTGAAG
TCTAGGTCCC
TATTTATTTT
TTTATAGTTA
TGTTAGTATT
AAGAACGTTA
TTTATATTTC
AAATTTTTCT
TTTTTTTCTG
TACAGACGCG
TGTACGCATG
TAACATTATA
CTGAAAACCT
TGCTTGAGAA
GGTTTTGGGA
CGCTCGAAGG
CTTTAATTTG
CGGCCGGTAC
CCAATTCGCC
CTATAGTGAG
TCGTATTACG
CGCGCTCACT
GGCCGTCGTT
TTACAACGTC
GTGACTGGGA
AAACCCTGGC
GTTACCCAAC
TTAATCGCCT
TGCAGCACAT
CCCCCTTTCG
CCAGCTGGCG
TAATAGCGAA
GAGGCCCGCA
CCGATCGCCC
TTCCCAACAG
TTGCGCAGCC
TGAATGGCGA
ATGGACGCGC
CCTGTAGCGG
CGCATTAAGC
GCGGCGGGTG
TGGTGGTTAC
GCGCAGCGTG
ACCGCTACAC
TTGCCAGCGC
CCTAGCGCCC
GCTCCTTTCG
CTTTCTTCCC
TTCCTTTCTC
GCCACGTTCG
CCGGCTTTCC
CCGTCAAGCT
CTAAATCGGG
GGCTCCCTTT
AGGGTTCCGA
TTTAGTGCTT
TACGGCACCT
CGACCCCAAA
AAACTTGATT
AGGGTGATGG
TTCACGTAGT
GGGCCATCGC
CCTGATAGAC
GGTTTTTCGC
CCTTTGACGT
TGGAGTCCAC
GTTCTTTAAT
AGTGGACTCT
TGTTCCAAAC
TGGAACAACA
CTCAACCCTA
TCTCGGTCTA
TTCTTTTGAT
TTATAAGGGA
TTTTGCCGAT
TTCGGCCTAT
TGGTTAAAAA
ATGAGCTGAT
TTAACAAAAA
TTTAACGCGA
ATTTTAACAA
AATATTAACG
CTTACAATTT
CCTGATGCGG
TATTTTCTCC
TTACGCATCT
GTGCGG