名称: | pKSE401-EGFP |
---|---|
别称: | |
基因长: | |
质粒宿主: | 植物 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 空载体 |
原核抗性: | Kan |
筛选标记: | Neo/G418 |
荧光标记: | 绿色 |
启动子: | AtU6-26p |
复制子: | pVS1 |
拷贝数: | 低 |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pKSE401 |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TAAACGCTCT
TTTCTCTTAG
GTTTACCCGC
CAATATATCC
TGTCAAACAC
TGATAGTTTA
AACTGAAGGC
GGGAAACGAC
AATCTGATCC
AAGCTCAAGC
TGCTCTAGCA
TTCGCCATTC
AGGCTGCGCA
ACTGTTGGGA
AGGGCGATCG
GTGCGGGCCT
CTTCGCTATT
ACGCCAGCTG
GCGAAAGGGG
GATGTGCTGC
AAGGCGATTA
AGTTGGGTAA
CGCCAGGGTT
TTCCCAGTCA
CGACGTTGTA
AAACGACGGC
CAGTGCCAAG
CTTCGACTTG
CCTTCCGCAC
AATACATCAT
TTCTTCTTAG
CTTTTTTTCT
TCTTCTTCGT
TCATACAGTT
TTTTTTTGTT
TATCAGCTTA
CATTTTCTTG
AACCGTAGCT
TTCGTTTTCT
TCTTTTTAAC
TTTCCATTCG
GAGTTTTTGT
ATCTTGTTTC
ATAGTTTGTC
CCAGGATTAG
AATGATTAGG
CATCGAACCT
TCAAGAATTT
GATTGAATAA
AACATCTTCA
TTCTTAAGAT
ATGAAGATAA
TCTTCAAAAG
GCCCCTGGGA
ATCTGAAAGA
AGAGAAGCAG
GCCCATTTAT
ATGGGAAAGA
ACAATAGTAT
TTCTTATATA
GGCCCATTTA
AGTTGAAAAC
AATCTTCAAA
AGTCCCACAT
CGCTTAGATA
AGAAAACGAA
GCTGAGTTTA
TATACAGCTA
GAGTCGAAGT
AGTGATTGGG
AGACCAACCC
AGTGGACATA
AGCCTGTTCG
GTTCGTAAGC
TGTAATGCAA
GTAGCGTATG
CGCTCACGCA
ACTGGTCCAG
AACCTTGACC
GAACGCAGCG
GTGGTAACGG
CGCAGTGGCG
GTTTTCATGG
CTTGTTATGA
CTGTTTTTTT
GGGGTACAGT
CTATGCCTCG
GGCATCCAAG
CAGCAAGCGC
GTTACGCCGT
GGGTCGATGT
TTGATGTTAT
GGAGCAGCAA
CGATGTTACG
CAGCAGGGCA
GTCGCCCTAA
AACAAAGTTA
AACATCATGG
GGGAAGCGGT
GATCGCCGAA
GTATCGACTC
AACTATCAGA
GGTAGTTGGC
GTCATCGAGC
GCCATCTCGA
ACCGACGTTG
CTGGCCGTAC
ATTTGTACGG
CTCCGCAGTG
GATGGCGGCC
TGAAGCCACA
CAGTGATATT
GATTTGCTGG
TTACGGTGAC
CGTAAGGCTT
GATGAAACAA
CGCGGCGAGC
TTTGATCAAC
GACCTTTTGG
AAACTTCGGC
TTCCCCTGGA
GAGAGCGAGA
TTCTCCGCGC
TGTAGAAGTC
ACCATTGTTG
TGCACGACGA
CATCATTCCG
TGGCGTTATC
CAGCTAAGCG
CGAACTGCAA
TTTGGAGAAT
GGCAGCGCAA
TGACATTCTT
GCAGGTATCT
TCGAGCCAGC
CACGATCGAC
ATTGATCTGG
CTATCTTGCT
GACAAAAGCA
AGAGAACATA
GCGTTGCCTT
GGTAGGTCCA
GCGGCGGAGG
AACTCTTTGA
TCCGGTTCCT
GAACAGGATC
TATTTGAGGC
GCTAAATGAA
ACCTTAACGC
TATGGAACTC
GCCGCCCGAC
TGGGCTGGCG
ATGAGCGAAA
TGTAGTGCTT
ACGTTGTCCC
GCATTTGGTA
CAGCGCAGTA
ACCGGCAAAA
TCGCGCCGAA
GGATGTCGCT
GCCGACTGGG
CAATGGAGCG
CCTGCCGGCC
CAGTATCAGC
CCGTCATACT
TGAAGCTAGA
CAGGCTTATC
TTGGACAAGA
AGAAGATCGC
TTGGCCTCGC
GCGCAGATCA
GTTGGAAGAA
TTTGTCCACT
ACGTGAAAGG
CGAGATCACC
AAGGTAGTCG
GCAAATAATG
TCTAGCTAGA
AATTCGTTCA
AGCCGACGCC
GCTTCGCGGC
GCGGCTTAAC
TCAAGCGTTA
GATGCACTAA
GCACATAATT
GCTCACAGCC
AAACTATCAG
GTCAAGTCTG
CTTTTATTAT
TTTTAAGCGT
GCATAATAAG
CCGGTCTCGG
TTTTAGAGCT
AGAAATAGCA
AGTTAAAATA
AGGCTAGTCC
GTTATCAACT
TGAAAAAGTG
GCACCGAGTC
GGTGCTTTTT
TTTGCAAAAT
TTTCCAGATC
GATTTCTTCT
TCCTCTGTTC
TTCGGCGTTC
AATTTCTGGG
GTTTTCTCTT
CGTTTTCTGT
AACTGAAACC
TAAAATTTGA
CCTAAAAAAA
ATCTCAAATA
ATATGATTCA
GTGGTTTTGT
ACTTTTCAGT
TAGTTGAGTT
TTGCAGTTCC
GATGAGATAA
ACCAATAAGC
TTGCATGCCT
GCAGGTCAAC
ATGGTGGAGC
ACGACACACT
TGTCTACTCC
AAAAATATCA
AAGATACAGT
CTCAGAAGAC
CAAAGGGCAA
TTGAGACTTT
TCAACAAAGG
GTAATATCCG
GAAACCTCCT
CGGATTCCAT
TGCCCAGCTA
TCTGTCACTT
TATTGTGAAG
ATAGTGGAAA
AGGAAGGTGG
CTCCTACAAA
TGCCATCATT
GCGATAAAGG
AAAGGCCATC
GTTGAAGATG
CCTCTGCCGA
CAGTGGTCCC
AAAGATGGAC
CCCCACCCAC
GAGGAGCATC
GTGGAAAAAG
AAGACGTTCC
AACCACGTCT
TCAAAGCAAG
TGGATTGATG
TGATAACATG
GTGGAGCACG
ACACACTTGT
CTACTCCAAA
AATATCAAAG
ATACAGTCTC
AGAAGACCAA
AGGGCAATTG
AGACTTTTCA
ACAAAGGGTA
ATATCCGGAA
ACCTCCTCGG
ATTCCATTGC
CCAGCTATCT
GTCACTTTAT
TGTGAAGATA
GTGGAAAAGG
AAGGTGGCTC
CTACAAATGC
CATCATTGCG
ATAAAGGAAA
GGCCATCGTT
GAAGATGCCT
CTGCCGACAG
TGGTCCCAAA
GATGGACCCC
CACCCACGAG
GAGCATCGTG
GAAAAAGAAG
ACGTTCCAAC
CACGTCTTCA
AAGCAAGTGG
ATTGATGTGA
TATCTCCACT
GACGTAAGGG
ATGACGCACA
ATCCCACTAT
CCTTCGCAAG
ACCCTTCCTC
TATATAAGGA
AGTTCATTTC
ATTTGGAGAG
GACCTCGACC
TCAACACAAC
ATATACAAAA
CAAACGAATC
TCAAGCAATC
AAGCATTCTA
CTTCTATTGC
AGCAATTTAA
ATCATTTCTT
TTAAAGCAAA
AGCAATTTTC
TGAAAATTTT
CACCATTTAC
GAACGATACT
CGAGTAATCT
AGATGGATTA
CAAGGACCAC
GACGGGGATT
ACAAGGACCA
CGACATTGAT
TACAAGGATG
ATGATGACAA
GATGGCTCCG
AAGAAGAAGA
GGAAGGTTGG
CATCCACGGG
GTGCCAGCTG
CTGACAAGAA
GTACTCGATC
GGCCTCGATA
TTGGGACTAA
CTCTGTTGGC
TGGGCCGTGA
TCACCGACGA
GTACAAGGTG
CCCTCAAAGA
AGTTCAAGGT
CCTGGGCAAC
ACCGATCGGC
ATTCCATCAA
GAAGAATCTC
ATTGGCGCTC
TCCTGTTCGA
CAGCGGCGAG
ACGGCTGAGG
CTACGCGGCT
CAAGCGCACC
GCCCGCAGGC
GGTACACGCG
CAGGAAGAAT
CGCATCTGCT
ACCTGCAGGA
GATTTTCTCC
AACGAGATGG
CGAAGGTTGA
CGATTCTTTC
TTCCACAGGC
TGGAGGAGTC
ATTCCTCGTG
GAGGAGGATA
AGAAGCACGA
GCGGCATCCA
ATCTTCGGCA
ACATTGTCGA
CGAGGTTGCC
TACCACGAGA
AGTACCCTAC
GATCTACCAT
CTGCGGAAGA
AGCTCGTGGA
CTCCACAGAT
AAGGCGGACC
TCCGCCTGAT
CTACCTCGCT
CTGGCCCACA
TGATTAAGTT
CAGGGGCCAT
TTCCTGATCG
AGGGGGATCT
CAACCCGGAC
AATAGCGATG
TTGACAAGCT
GTTCATCCAG
CTCGTGCAGA
CGTACAACCA
GCTCTTCGAG
GAGAACCCCA
TTAATGCGTC
AGGCGTCGAC
GCGAAGGCTA
TCCTGTCCGC
TAGGCTCTCG
AAGTCTCGGC
GCCTCGAGAA
CCTGATCGCC
CAGCTGCCGG
GCGAGAAGAA
GAACGGCCTG
TTCGGGAATC
TCATTGCGCT
CAGCCTGGGG
CTCACGCCCA
ACTTCAAGTC
GAATTTCGAT
CTCGCTGAGG
ACGCCAAGCT
GCAGCTCTCC
AAGGACACAT
ACGACGATGA
CCTGGATAAC
CTCCTGGCCC
AGATCGGCGA
TCAGTACGCG
GACCTGTTCC
TCGCTGCCAA
GAATCTGTCG
GACGCCATCC
TCCTGTCTGA
TATTCTCAGG
GTGAACACCG
AGATTACGAA
GGCTCCGCTC
TCAGCCTCCA
TGATCAAGCG
CTACGACGAG
CACCATCAGG
ATCTGACCCT
CCTGAAGGCG
CTGGTCAGGC
AGCAGCTCCC
CGAGAAGTAC
AAGGAGATCT
TCTTCGATCA
GTCGAAGAAC
GGCTACGCTG
GGTACATTGA
CGGCGGGGCC
TCTCAGGAGG
AGTTCTACAA
GTTCATCAAG
CCGATTCTGG
AGAAGATGGA
CGGCACGGAG
GAGCTGCTGG
TGAAGCTCAA
TCGCGAGGAC
CTCCTGAGGA
AGCAGCGGAC
ATTCGATAAC
GGCAGCATCC
CACACCAGAT
TCATCTCGGG
GAGCTGCACG
CTATCCTGAG
GAGGCAGGAG
GACTTCTACC
CTTTCCTCAA
GGATAACCGC
GAGAAGATCG
AGAAGATTCT
GACTTTCAGG
ATCCCGTACT
ACGTCGGCCC
ACTCGCTAGG
GGCAACTCCC
GCTTCGCTTG
GATGACCCGC
AAGTCAGAGG
AGACGATCAC
GCCGTGGAAC
TTCGAGGAGG
TGGTCGACAA
GGGCGCTAGC
GCTCAGTCGT
TCATCGAGAG
GATGACGAAT
TTCGACAAGA
ACCTGCCAAA
TGAGAAGGTG
CTCCCTAAGC
ACTCGCTCCT
GTACGAGTAC
TTCACAGTCT
ACAACGAGCT
GACTAAGGTG
AAGTATGTGA
CCGAGGGCAT
GAGGAAGCCG
GCTTTCCTGT
CTGGGGAGCA
GAAGAAGGCC
ATCGTGGACC
TCCTGTTCAA
GACCAACCGG
AAGGTCACGG
TTAAGCAGCT
CAAGGAGGAC
TACTTCAAGA
AGATTGAGTG
CTTCGATTCG
GTCGAGATCT
CTGGCGTTGA
GGACCGCTTC
AACGCCTCCC
TGGGGACCTA
CCACGATCTC
CTGAAGATCA
TTAAGGATAA
GGACTTCCTG
GACAACGAGG
AGAATGAGGA
TATCCTCGAG
GACATTGTGC
TGACACTCAC
TCTGTTCGAG
GACCGGGAGA
TGATCGAGGA
GCGCCTGAAG
ACTTACGCCC
ATCTCTTCGA
TGACAAGGTC
ATGAAGCAGC
TCAAGAGGAG
GAGGTACACC
GGCTGGGGGA
GGCTGAGCAG
GAAGCTCATC
AACGGCATTC
GGGACAAGCA
GTCCGGGAAG
ACGATCCTCG
ACTTCCTGAA
GAGCGATGGC
TTCGCGAACC
GCAATTTCAT
GCAGCTGATT
CACGATGACA
GCCTCACATT
CAAGGAGGAT
ATCCAGAAGG
CTCAGGTGAG
CGGCCAGGGG
GACTCGCTGC
ACGAGCATAT
CGCGAACCTC
GCTGGCTCGC
CAGCTATCAA
GAAGGGGATT
CTGCAGACCG
TGAAGGTTGT
GGACGAGCTG
GTGAAGGTCA
TGGGCAGGCA
CAAGCCTGAG
AACATCGTCA
TTGAGATGGC
CCGGGAGAAT
CAGACCACGC
AGAAGGGCCA
GAAGAACTCA
CGCGAGAGGA
TGAAGAGGAT
CGAGGAGGGC
ATTAAGGAGC
TGGGGTCCCA
GATCCTCAAG
GAGCACCCGG
TGGAGAACAC
GCAGCTGCAG
AATGAGAAGC
TCTACCTGTA
CTACCTCCAG
AATGGCCGCG
ATATGTATGT
GGACCAGGAG
CTGGATATTA
ACAGGCTCAG
CGATTACGAC
GTCGATCATA
TCGTTCCACA
GTCATTCCTG
AAGGATGACT
CCATTGACAA
CAAGGTCCTC
ACCAGGTCGG
ACAAGAACCG
GGGCAAGTCT
GATAATGTTC
CTTCAGAGGA
GGTCGTTAAG
AAGATGAAGA
ACTACTGGCG
CCAGCTCCTG
AATGCCAAGC
TGATCACGCA
GCGGAAGTTC
GATAACCTCA
CAAAGGCTGA
GAGGGGCGGG
CTCTCTGAGC
TGGACAAGGC
GGGCTTCATC
AAGAGGCAGC
TGGTCGAGAC
ACGGCAGATC
ACTAAGCACG
TTGCGCAGAT
TCTCGACTCA
CGGATGAACA
CTAAGTACGA
TGAGAATGAC
AAGCTGATCC
GCGAGGTGAA
GGTCATCACC
CTGAAGTCAA
AGCTCGTCTC
CGACTTCAGG
AAGGATTTCC
AGTTCTACAA
GGTTCGGGAG
ATCAACAATT
ACCACCATGC
CCATGACGCG
TACCTGAACG
CGGTGGTCGG
CACAGCTCTG
ATCAAGAAGT
ACCCAAAGCT
CGAGAGCGAG
TTCGTGTACG
GGGACTACAA
GGTTTACGAT
GTGAGGAAGA
TGATCGCCAA
GTCGGAGCAG
GAGATTGGCA
AGGCTACCGC
CAAGTACTTC
TTCTACTCTA
ACATTATGAA
TTTCTTCAAG
ACAGAGATCA
CTCTGGCCAA
TGGCGAGATC
CGGAAGCGCC
CCCTCATCGA
GACGAACGGC
GAGACGGGGG
AGATCGTGTG
GGACAAGGGC
AGGGATTTCG
CGACCGTCAG
GAAGGTTCTC
TCCATGCCAC
AAGTGAATAT
CGTCAAGAAG
ACAGAGGTCC
AGACTGGCGG
GTTCTCTAAG
GAGTCAATTC
TGCCTAAGCG
GAACAGCGAC
AAGCTCATCG
CCCGCAAGAA
GGACTGGGAT
CCGAAGAAGT
ACGGCGGGTT
CGACAGCCCC
ACTGTGGCCT
ACTCGGTCCT
GGTTGTGGCG
AAGGTTGAGA
AGGGCAAGTC
CAAGAAGCTC
AAGAGCGTGA
AGGAGCTGCT
GGGGATCACG
ATTATGGAGC
GCTCCAGCTT
CGAGAAGAAC
CCGATCGATT
TCCTGGAGGC
GAAGGGCTAC
AAGGAGGTGA
AGAAGGACCT
GATCATTAAG
CTCCCCAAGT
ACTCACTCTT
CGAGCTGGAG
AACGGCAGGA
AGCGGATGCT
GGCTTCCGCT
GGCGAGCTGC
AGAAGGGGAA
CGAGCTGGCT
CTGCCGTCCA
AGTATGTGAA
CTTCCTCTAC
CTGGCCTCCC
ACTACGAGAA
GCTCAAGGGC
AGCCCCGAGG
ACAACGAGCA
GAAGCAGCTG
TTCGTCGAGC
AGCACAAGCA
TTACCTCGAC
GAGATCATTG
AGCAGATTTC
CGAGTTCTCC
AAGCGCGTGA
TCCTGGCCGA
CGCGAATCTG
GATAAGGTCC
TCTCCGCGTA
CAACAAGCAC
CGCGACAAGC
CAATCAGGGA
GCAGGCTGAG
AATATCATTC
ATCTCTTCAC
CCTGACGAAC
CTCGGCGCCC
CTGCTGCTTT
CAAGTACTTC
GACACAACTA
TCGATCGCAA
GAGGTACACA
AGCACTAAGG
AGGTCCTGGA
CGCGACCCTC
ATCCACCAGT
CGATTACCGG
CCTCTACGAG
ACGCGCATCG
ACCTGTCTCA
GCTCGGGGGC
GACAAGCGGC
CAGCGGCGAC
GAAGAAGGCG
GGGCAGGCGA
AGAAGAAGAA
GGGATCCGGC
GCAACAAACT
TCTCTCTGCT
GAAACAAGCC
GGAGATGTCG
AAGAGAATCC
TGGACCGATG
GTGTCTAAGG
GCGAAGAGCT
GTTCACCGGG
GTGGTGCCCA
TCCTGGTCGA
GCTGGACGGC
GACGTAAACG
GCCACAAGTT
CAGCGTGTCC
GGCGAGGGCG
AGGGCGATGC
CACCTACGGC
AAGCTGACCC
TGAAGTTCAT
CTGCACCACC
GGCAAGCTGC
CCGTGCCCTG
GCCCACCCTC
GTGACCACCC
TGACCTACGG
CGTGCAGTGC
TTCAGCCGCT
ACCCCGACCA
CATGAAGCAG
CACGACTTCT
TCAAGTCCGC
CATGCCCGAA
GGCTACGTCC
AGGAGCGCAC
CATCTTCTTC
AAGGACGACG
GCAACTACAA
GACCCGCGCC
GAGGTGAAGT
TCGAGGGCGA
CACCCTGGTG
AACCGCATCG
AGCTGAAGGG
CATCGACTTC
AAGGAGGACG
GCAACATCCT
GGGGCACAAG
CTGGAGTACA
ACTACAACAG
CCACAACGTC
TATATCATGG
CCGACAAGCA
GAAGAACGGC
ATCAAGGTGA
ACTTCAAGAT
CCGCCACAAC
ATCGAGGACG
GCAGCGTGCA
GCTCGCCGAC
CACTACCAGC
AGAACACCCC
CATCGGCGAC
GGCCCCGTGC
TGCTGCCCGA
CAACCACTAC
CTGAGCACCC
AGTCCGCCCT
GAGCAAAGAC
CCCAACGAGA
AGCGCGATCA
CATGGTCCTG
CTGGAGTTCG
TGACCGCCGC
CGGGATCACT
CTCGGCATGG
ACGAGCTGTA
CAAGTGAGCT
CAGAGCTTTC
GTTCGTATCA
TCGGTTTCGA
CAACGTTCGT
CAAGTTCAAT
GCATCAGTTT
CATTGCGCAC
ACACCAGAAT
CCTACTGAGT
TTGAGTATTA
TGGCATTGGG
AAAACTGTTT
TTCTTGTACC
ATTTGTTGTG
CTTGTAATTT
ACTGTGTTTT
TTATTCGGTT
TTCGCTATCG
AACTGTGAAA
TGGAAATGGA
TGGAGAAGAG
TTAATGAATG
ATATGGTCCT
TTTGTTCATT
CTCAAATTAA
TATTATTTGT
TTTTTCTCTT
ATTTGTTGTG
TGTTGAATTT
GAAATTATAA
GAGATATGCA
AACATTTTGT
TTTGAGTAAA
AATGTGTCAA
ATCGTGGCCT
CTAATGACCG
AAGTTAATAT
GAGGAGTAAA
ACACTTGTAG
TTGTACCATT
ATGCTTATTC
ACTAGGCAAC
AAATATATTT
TCAGACCTAG
AAAAGCTGCA
AATGTTACTG
AATACAAGTA
TGTCCTCTTG
TGTTTTAGAC
ATTTATGAAC
TTTCCTTTAT
GTAATTTTCC
AGAATCCTTG
TCAGATTCTA
ATCATTGCTT
TATAATTATA
GTTATACTCA
TGGATTTGTA
GTTGAGTATG
AAAATATTTT
TTAATGCATT
TTATGACTTG
CCAATTGATT
GACAACGAAT
TCGTAATCAT
GTCATAGCTG
TTTCCTGTGT
GAAATTGTTA
TCCGCTCACA
ATTCCACACA
ACATACGAGC
CGGAAGCATA
AAGTGTAAAG
CCTGGGGTGC
CTAATGAGTG
AGCTAACTCA
CATTAATTGC
GTTGCGCTCA
CTGCCCGCTT
TCCAGTCGGG
AAACCTGTCG
TGCCAGCTGC
ATTAATGAAT
CGGCCAACGC
GCGGGGAGAG
GCGGTTTGCG
TATTGGCTAG
AGCAGCTTGC
CAACATGGTG
GAGCACGACA
CTCTCGTCTA
CTCCAAGAAT
ATCAAAGATA
CAGTCTCAGA
AGACCAAAGG
GCTATTGAGA
CTTTTCAACA
AAGGGTAATA
TCGGGAAACC
TCCTCGGATT
CCATTGCCCA
GCTATCTGTC
ACTTCATCAA
AAGGACAGTA
GAAAAGGAAG
GTGGCACCTA
CAAATGCCAT
CATTGCGATA
AAGGAAAGGC
TATCGTTCAA
GATGCCTCTG
CCGACAGTGG
TCCCAAAGAT
GGACCCCCAC
CCACGAGGAG
CATCGTGGAA
AAAGAAGACG
TTCCAACCAC
GTCTTCAAAG
CAAGTGGATT
GATGTGAACA
TGGTGGAGCA
CGACACTCTC
GTCTACTCCA
AGAATATCAA
AGATACAGTC
TCAGAAGACC
AAAGGGCTAT
TGAGACTTTT
CAACAAAGGG
TAATATCGGG
AAACCTCCTC
GGATTCCATT
GCCCAGCTAT
CTGTCACTTC
ATCAAAAGGA
CAGTAGAAAA
GGAAGGTGGC
ACCTACAAAT
GCCATCATTG
CGATAAAGGA
AAGGCTATCG
TTCAAGATGC
CTCTGCCGAC
AGTGGTCCCA
AAGATGGACC
CCCACCCACG
AGGAGCATCG
TGGAAAAAGA
AGACGTTCCA
ACCACGTCTT
CAAAGCAAGT
GGATTGATGT
GATATCTCCA
CTGACGTAAG
GGATGACGCA
CAATCCCACT
ATCCTTCGCA
AGACCCTTCC
TCTATATAAG
GAAGTTCATT
TCATTTGGAG
AGGACACGCT
GAAATCACCA
GTCTCTCTCT
ACAAATCTAT
CTCTCTCGAG
CTTTCGCAGA
TCTGTCGATC
GACCATGGGG
ATTGAACAAG
ATGGATTGCA
CGCAGGTTCT
CCGGCCGCTT
GGGTGGAGAG
GCTATTCGGC
TATGACTGGG
CACAACAGAC
AATCGGCTGC
TCTGATGCCG
CCGTGTTTCG
GCTGTCAGCG
CAGGGGCGCC
CGGTTCTTTT
TGTCAAGACC
GACCTGTCCG
GTGCCCTGAA
TGAACTCCAG
GACGAGGCAG
CGCGGCTATC
GTGGCTGGCC
ACGACGGGCG
TTCCTTGCGC
AGCTGTGCTC
GACGTTGTCA
CTGAAGCGGG
AAGGGACTGG
CTGCTATTGG
GCGAAGTGCC
GGGGCAGGAT
CTCCTGTCAT
CTCACCTTGC
TCCTGCCGAG
AAAGTATCCA
TCATGGCTGA
TGCAATGCGG
CGGCTGCATA
CGCTTGATCC
GGCTACCTGC
CCATTCGACC
ACCAAGCGAA
ACATCGCATC
GAGCGAGCAC
GTACTCGGAT
GGAAGCCGGT
CTTGTCGATC
AGGATGATCT
GGACGAAGAG
CATCAGGGGC
TCGCGCCAGC
CGAACTGTTC
GCCAGGCTCA
AGGCGCGCAT
GCCCGACGGC
GAGGATCTCG
TCGTGACACA
TGGCGATGCC
TGCTTGCCGA
ATATCATGGT
GGAAAATGGC
CGCTTTTCTG
GATTCATCGA
CTGTGGCCGG
CTGGGTGTGG
CGGACCGCTA
TCAGGACATA
GCGTTGGCTA
CCCGTGATAT
TGCTGAAGAG
CTTGGCGGCG
AATGGGCTGA
CCGCTTCCTC
GTGCTTTACG
GTATCGCCGC
TCCCGATTCG
CAGCGCATCG
CCTTCTATCG
CCTTCTTGAC
GAGTTCTTCT
GAGCGGGACT
CTGGGGTTCG
GATCGATCCT
CTAGCTAGAG
TCGATCGACA
AGCTCGAGTT
TCTCCATAAT
AATGTGTGAG
TAGTTCCCAG
ATAAGGGAAT
TAGGGTTCCT
ATAGGGTTTC
GCTCATGTGT
TGAGCATATA
AGAAACCCTT
AGTATGTATT
TGTATTTGTA
AAATACTTCT
ATCAATAAAA
TTTCTAATTC
CTAAAACCAA
AATCCAGTAC
TAAAATCCAG
ATCCCCCGAA
TTAATTCGGC
GTTAATTCAG
TACATTAAAA
ACGTCCGCAA
TGTGTTATTA
AGTTGTCTAA
GCGTCAATTT
GTTTACACCA
CAATATATCC
TGCCACCAGC
CAGCCAACAG
CTCCCCGACC
GGCAGCTCGG
CACAAAATCA
CCACTCGATA
CAGGCAGCCC
ATCAGTCCGG
GACGGCGTCA
GCGGGAGAGC
CGTTGTAAGG
CGGCAGACTT
TGCTCATGTT
ACCGATGCTA
TTCGGAAGAA
CGGCAACTAA
GCTGCCGGGT
TTGAAACACG
GATGATCTCG
CGGAGGGTAG
CATGTTGATT
GTAACGATGA
CAGAGCGTTG
CTGCCTGTGA
TCACCGCGGT
TTCAAAATCG
GCTCCGTCGA
TACTATGTTA
TACGCCAACT
TTGAAAACAA
CTTTGAAAAA
GCTGTTTTCT
GGTATTTAAG
GTTTTAGAAT
GCAAGGAACA
GTGAATTGGA
GTTCGTCTTG
TTATAATTAG
CTTCTTGGGG
TATCTTTAAA
TACTGTAGAA
AAGAGGAAGG
AAATAATAAA
TGGCTAAAAT
GAGAATATCA
CCGGAATTGA
AAAAACTGAT
CGAAAAATAC
CGCTGCGTAA
AAGATACGGA
AGGAATGTCT
CCTGCTAAGG
TATATAAGCT
GGTGGGAGAA
AATGAAAACC
TATATTTAAA
AATGACGGAC
AGCCGGTATA
AAGGGACCAC
CTATGATGTG
GAACGGGAAA
AGGACATGAT
GCTATGGCTG
GAAGGAAAGC
TGCCTGTTCC
AAAGGTCCTG
CACTTTGAAC
GGCATGATGG
CTGGAGCAAT
CTGCTCATGA
GTGAGGCCGA
TGGCGTCCTT
TGCTCGGAAG
AGTATGAAGA
TGAACAAAGC
CCTGAAAAGA
TTATCGAGCT
GTATGCGGAG
TGCATCAGGC
TCTTTCACTC
CATCGACATA
TCGGATTGTC
CCTATACGAA
TAGCTTAGAC
AGCCGCTTAG
CCGAATTGGA
TTACTTACTG
AATAACGATC
TGGCCGATGT
GGATTGCGAA
AACTGGGAAG
AAGACACTCC
ATTTAAAGAT
CCGCGCGAGC
TGTATGATTT
TTTAAAGACG
GAAAAGCCCG
AAGAGGAACT
TGTCTTTTCC
CACGGCGACC
TGGGAGACAG
CAACATCTTT
GTGAAAGATG
GCAAAGTAAG
TGGCTTTATT
GATCTTGGGA
GAAGCGGCAG
GGCGGACAAG
TGGTATGACA
TTGCCTTCTG
CGTCCGGTCG
ATCAGGGAGG
ATATCGGGGA
AGAACAGTAT
GTCGAGCTAT
TTTTTGACTT
ACTGGGGATC
AAGCCTGATT
GGGAGAAAAT
AAAATATTAT
ATTTTACTGG
ATGAATTGTT
TTAGTACCTA
GAATGCATGA
CCAAAATCCC
TTAACGTGAG
TTTTCGTTCC
ACTGAGCGTC
AGACCCCGTA
GAAAAGATCA
AAGGATCTTC
TTGAGATCCT
TTTTTTCTGC
GCGTAATCTG
CTGCTTGCAA
ACAAAAAAAC
CACCGCTACC
AGCGGTGGTT
TGTTTGCCGG
ATCAAGAGCT
ACCAACTCTT
TTTCCGAAGG
TAACTGGCTT
CAGCAGAGCG
CAGATACCAA
ATACTGTCCT
TCTAGTGTAG
CCGTAGTTAG
GCCACCACTT
CAAGAACTCT
GTAGCACCGC
CTACATACCT
CGCTCTGCTA
ATCCTGTTAC
CAGTGGCTGC
TGCCAGTGGC
GATAAGTCGT
GTCTTACCGG
GTTGGACTCA
AGACGATAGT
TACCGGATAA
GGCGCAGCGG
TCGGGCTGAA
CGGGGGGTTC
GTGCACACAG
CCCAGCTTGG
AGCGAACGAC
CTACACCGAA
CTGAGATACC
TACAGCGTGA
GCTATGAGAA
AGCGCCACGC
TTCCCGAAGG
GAGAAAGGCG
GACAGGTATC
CGGTAAGCGG
CAGGGTCGGA
ACAGGAGAGC
GCACGAGGGA
GCTTCCAGGG
GGAAACGCCT
GGTATCTTTA
TAGTCCTGTC
GGGTTTCGCC
ACCTCTGACT
TGAGCGTCGA
TTTTTGTGAT
GCTCGTCAGG
GGGGCGGAGC
CTATGGAAAA
ACGCCAGCAA
CGCGGCCTTT
TTACGGTTCC
TGGCCTTTTG
CTGGCCTTTT
GCTCACATGT
TCTTTCCTGC
GTTATCCCCT
GATTCTGTGG
ATAACCGTAT
TACCGCCTTT
GAGTGAGCTG
ATACCGCTCG
CCGCAGCCGA
ACGACCGAGC
GCAGCGAGTC
AGTGAGCGAG
GAAGCGGAAG
AGCGCCTGAT
GCGGTATTTT
CTCCTTACGC
ATCTGTGCGG
TATTTCACAC
CGCATATGGT
GCACTCTCAG
TACAATCTGC
TCTGATGCCG
CATAGTTAAG
CCAGTATACA
CTCCGCTATC
GCTACGTGAC
TGGGTCATGG
CTGCGCCCCG
ACACCCGCCA
ACACCCGCTG
ACGCGCCCTG
ACGGGCTTGT
CTGCTCCCGG
CATCCGCTTA
CAGACAAGCT
GTGACCGTCT
CCGGGAGCTG
CATGTGTCAG
AGGTTTTCAC
CGTCATCACC
GAAACGCGCG
AGGCAGGGTG
CCTTGATGTG
GGCGCCGGCG
GTCGAGTGGC
GACGGCGCGG
CTTGTCCGCG
CCCTGGTAGA
TTGCCTGGCC
GTAGGCCAGC
CATTTTTGAG
CGGCCAGCGG
CCGCGATAGG
CCGACGCGAA
GCGGCGGGGC
GTAGGGAGCG
CAGCGACCGA
AGGGTAGGCG
CTTTTTGCAG
CTCTTCGGCT
GTGCGCTGGC
CAGACAGTTA
TGCACAGGCC
AGGCGGGTTT
TAAGAGTTTT
AATAAGTTTT
AAAGAGTTTT
AGGCGGAAAA
ATCGCCTTTT
TTCTCTTTTA
TATCAGTCAC
TTACATGTGT
GACCGGTTCC
CAATGTACGG
CTTTGGGTTC
CCAATGTACG
GGTTCCGGTT
CCCAATGTAC
GGCTTTGGGT
TCCCAATGTA
CGTGCTATCC
ACAGGAAACA
GACCTTTTCG
ACCTTTTTCC
CCTGCTAGGG
CAATTTGCCC
TAGCATCTGC
TCCGTACATT
AGGAACCGGC
GGATGCTTCG
CCCTCGATCA
GGTTGCGGTA
GCGCATGACT
AGGATCGGGC
CAGCCTGCCC
CGCCTCCTCC
TTCAAATCGT
ACTCCGGCAG
GTCATTTGAC
CCGATCAGCT
TGCGCACGGT
GAAACAGAAC
TTCTTGAACT
CTCCGGCGCT
GCCACTGCGT
TCGTAGATCG
TCTTGAACAA
CCATCTGGCT
TCTGCCTTGC
CTGCGGCGCG
GCGTGCCAGG
CGGTAGAGAA
AACGGCCGAT
GCCGGGATCG
ATCAAAAAGT
AATCGGGGTG
AACCGTCAGC
ACGTCCGGGT
TCTTGCCTTC
TGTGATCTCG
CGGTACATCC
AATCAGCTAG
CTCGATCTCG
ATGTACTCCG
GCCGCCCGGT
TTCGCTCTTT
ACGATCTTGT
AGCGGCTAAT
CAAGGCTTCA
CCCTCGGATA
CCGTCACCAG
GCGGCCGTTC
TTGGCCTTCT
TCGTACGCTG
CATGGCAACG
TGCGTGGTGT
TTAACCGAAT
GCAGGTTTCT
ACCAGGTCGT
CTTTCTGCTT
TCCGCCATCG
GCTCGCCGGC
AGAACTTGAG
TACGTCCGCA
ACGTGTGGAC
GGAACACGCG
GCCGGGCTTG
TCTCCCTTCC
CTTCCCGGTA
TCGGTTCATG
GATTCGGTTA
GATGGGAAAC
CGCCATCAGT
ACCAGGTCGT
AATCCCACAC
ACTGGCCATG
CCGGCCGGCC
CTGCGGAAAC
CTCTACGTGC
CCGTCTGGAA
GCTCGTAGCG
GATCACCTCG
CCAGCTCGTC
GGTCACGCTT
CGACAGACGG
AAAACGGCCA
CGTCCATGAT
GCTGCGACTA
TCGCGGGTGC
CCACGTCATA
GAGCATCGGA
ACGAAAAAAT
CTGGTTGCTC
GTCGCCCTTG
GGCGGCTTCC
TAATCGACGG
CGCACCGGCT
GCCGGCGGTT
GCCGGGATTC
TTTGCGGATT
CGATCAGCGG
CCGCTTGCCA
CGATTCACCG
GGGCGTGCTT
CTGCCTCGAT
GCGTTGCCGC
TGGGCGGCCT
GCGCGGCCTT
CAACTTCTCC
ACCAGGTCAT
CACCCAGCGC
CGCGCCGATT
TGTACCGGGC
CGGATGGTTT
GCGACCGCTC
ACGCCGATTC
CTCGGGCTTG
GGGGTTCCAG
TGCCATTGCA
GGGCCGGCAG
GCAACCCAGC
CGCTTACGCC
TGGCCAACCG
CCCGTTCCTC
CACACATGGG
GCATTCCACG
GCGTCGGTGC
CTGGTTGTTC
TTGATTTTCC
ATGCCGCCTC
CTTTAGCCGC
TAAAATTCAT
CTACTCATTT
ATTCATTTGC
TCATTTACTC
TGGTAGCTGC
GCGATGTATT
CAGATAGCAG
CTCGGTAATG
GTCTTGCCTT
GGCGTACCGC
GTACATCTTC
AGCTTGGTGT
GATCCTCCGC
CGGCAACTGA
AAGTTGACCC
GCTTCATGGC
TGGCGTGTCT
GCCAGGCTGG
CCAACGTTGC
AGCCTTGCTG
CTGCGTGCGC
TCGGACGGCC
GGCACTTAGC
GTGTTTGTGC
TTTTGCTCAT
TTTCTCTTTA
CCTCATTAAC
TCAAATGAGT
TTTGATTTAA
TTTCAGCGGC
CAGCGCCTGG
ACCTCGCGGG
CAGCGTCGCC
CTCGGGTTCT
GATTCAAGAA
CGGTTGTGCC
GGCGGCGGCA
GTGCCTGGGT
AGCTCACGCG
CTGCGTGATA
CGGGACTCAA
GAATGGGCAG
CTCGTACCCG
GCCAGCGCCT
CGGCAACCTC
ACCGCCGATG
CGCGTGCCTT
TGATCGCCCG
CGACACGACA
AAGGCCGCTT
GTAGCCTTCC
ATCCGTGACC
TCAATGCGCT
GCTTAACCAG
CTCCACCAGG
TCGGCGGTGG
CCCATATGTC
GTAAGGGCTT
GGCTGCACCG
GAATCAGCAC
GAAGTCGGCT
GCCTTGATCG
CGGACACAGC
CAAGTCCGCC
GCCTGGGGCG
CTCCGTCGAT
CACTACGAAG
TCGCGCCGGC
CGATGGCCTT
CACGTCGCGG
TCAATCGTCG
GGCGGTCGAT
GCCGACAACG
GTTAGCGGTT
GATCTTCCCG
CACGGCCGCC
CAATCGCGGG
CACTGCCCTG
GGGATCGGAA
TCGACTAACA
GAACATCGGC
CCCGGCGAGT
TGCAGGGCGC
GGGCTAGATG
GGTTGCGATG
GTCGTCTTGC
CTGACCCGCC
TTTCTGGTTA
AGTACAGCGA
TAACCTTCAT
GCGTTCCCCT
TGCGTATTTG
TTTATTTACT
CATCGCATCA
TATACGCAGC
GACCGCATGA
CGCAAGCTGT
TTTACTCAAA
TACACATCAC
CTTTTTAGAC
GGCGGCGCTC
GGTTTCTTCA
GCGGCCAAGC
TGGCCGGCCA
GGCCGCCAGC
TTGGCATCAG
ACAAACCGGC
CAGGATTTCA
TGCAGCCGCA
CGGTTGAGAC
GTGCGCGGGC
GGCTCGAACA
CGTACCCGGC
CGCGATCATC
TCCGCCTCGA
TCTCTTCGGT
AATGAAAAAC
GGTTCGTCCT
GGCCGTCCTG
GTGCGGTTTC
ATGCTTGTTC
CTCTTGGCGT
TCATTCTCGG
CGGCCGCCAG
GGCGTCGGCC
TCGGTCAATG
CGTCCTCACG
GAAGGCACCG
CGCCGCCTGG
CCTCGGTGGG
CGTCACTTCC
TCGCTGCGCT
CAAGTGCGCG
GTACAGGGTC
GAGCGATGCA
CGCCAAGCAG
TGCAGCCGCC
TCTTTCACGG
TGCGGCCTTC
CTGGTCGATC
AGCTCGCGGG
CGTGCGCGAT
CTGTGCCGGG
GTGAGGGTAG
GGCGGGGGCC
AAACTTCACG
CCTCGGGCCT
TGGCGGCCTC
GCGCCCGCTC
CGGGTGCGGT
CGATGATTAG
GGAACGCTCG
AACTCGGCAA
TGCCGGCGAA
CACGGTCAAC
ACCATGCGGC
CGGCCGGCGT
GGTGGTGTCG
GCCCACGGCT
CTGCCAGGCT
ACGCAGGCCC
GCGCCGGCCT
CCTGGATGCG
CTCGGCAATG
TCCAGTAGGT
CGCGGGTGCT
GCGGGCCAGG
CGGTCTAGCC
TGGTCACTGT
CACAACGTCG
CCAGGGCGTA
GGTGGTCAAG
CATCCTGGCC
AGCTCCGGGC
GGTCGCGCCT
GGTGCCGGTG
ATCTTCTCGG
AAAACAGCTT
GGTGCAGCCG
GCCGCGTGCA
GTTCGGCCCG
TTGGTTGGTC
AAGTCCTGGT
CGTCGGTGCT
GACGCGGGCA
TAGCCCAGCA
GGCCAGCGGC
GGCGCTCTTG
TTCATGGCGT
AATGTCTCCG
GTTCTAGTCG
CAAGTATTCT
ACTTTATGCG
ACTAAAACAC
GCGACAAGAA
AACGCCAGGA
AAAGGGCAGG
GCGGCAGCCT
GTCGCGTAAC
TTAGGACTTG
TGCGACATGT
CGTTTTCAGA
AGACGGCTGC
ACTGAACGTC
AGAAGCCGAC
TGCACTATAG
CAGCGGAGGG
GTTGGATCAA
AGTACTTTGA
TCCCGAGGGG
AACCCTGTGG
TTGGCATGCA
CATACAAATG
GACGAACGGA
TAAACCTTTT
CACGCCCTTT
TAAATATCCG
ATTATTCTAA