名称: | pLV3-U6-MLH1(mouse)-sgRNA2-Cas9-Puro |
---|---|
别称: | |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | U6 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | LentiCRISPR V2 |
正向引物: | U6-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTCGACGGAT
CGGGAGATCT
CCCGATCCCC
TATGGTGCAC
TCTCAGTACA
ATCTGCTCTG
ATGCCGCATA
GTTAAGCCAG
TATCTGCTCC
CTGCTTGTGT
GTTGGAGGTC
GCTGAGTAGT
GCGCGAGCAA
AATTTAAGCT
ACAACAAGGC
AAGGCTTGAC
CGACAATTGC
ATGAAGAATC
TGCTTAGGGT
TAGGCGTTTT
GCGCTGCTTC
GCGATGTACG
GGCCAGATAT
ACGCGTTGAC
ATTGATTATT
GACTAGTTAT
TAATAGTAAT
CAATTACGGG
GTCATTAGTT
CATAGCCCAT
ATATGGAGTT
CCGCGTTACA
TAACTTACGG
TAAATGGCCC
GCCTGGCTGA
CCGCCCAACG
ACCCCCGCCC
ATTGACGTCA
ATAATGACGT
ATGTTCCCAT
AGTAACGCCA
ATAGGGACTT
TCCATTGACG
TCAATGGGTG
GAGTATTTAC
GGTAAACTGC
CCACTTGGCA
GTACATCAAG
TGTATCATAT
GCCAAGTACG
CCCCCTATTG
ACGTCAATGA
CGGTAAATGG
CCCGCCTGGC
ATTATGCCCA
GTACATGACC
TTATGGGACT
TTCCTACTTG
GCAGTACATC
TACGTATTAG
TCATCGCTAT
TACCATGGTG
ATGCGGTTTT
GGCAGTACAT
CAATGGGCGT
GGATAGCGGT
TTGACTCACG
GGGATTTCCA
AGTCTCCACC
CCATTGACGT
CAATGGGAGT
TTGTTTTGGC
ACCAAAATCA
ACGGGACTTT
CCAAAATGTC
GTAACAACTC
CGCCCCATTG
ACGCAAATGG
GCGGTAGGCG
TGTACGGTGG
GAGGTCTATA
TAAGCAGCGC
GTTTTGCCTG
TACTGGGTCT
CTCTGGTTAG
ACCAGATCTG
AGCCTGGGAG
CTCTCTGGCT
AACTAGGGAA
CCCACTGCTT
AAGCCTCAAT
AAAGCTTGCC
TTGAGTGCTT
CAAGTAGTGT
GTGCCCGTCT
GTTGTGTGAC
TCTGGTAACT
AGAGATCCCT
CAGACCCTTT
TAGTCAGTGT
GGAAAATCTC
TAGCAGTGGC
GCCCGAACAG
GGACTTGAAA
GCGAAAGGGA
AACCAGAGGA
GCTCTCTCGA
CGCAGGACTC
GGCTTGCTGA
AGCGCGCACG
GCAAGAGGCG
AGGGGCGGCG
ACTGGTGAGT
ACGCCAAAAA
TTTTGACTAG
CGGAGGCTAG
AAGGAGAGAG
ATGGGTGCGA
GAGCGTCAGT
ATTAAGCGGG
GGAGAATTAG
ATCGCGATGG
GAAAAAATTC
GGTTAAGGCC
AGGGGGAAAG
AAAAAATATA
AATTAAAACA
TATAGTATGG
GCAAGCAGGG
AGCTAGAACG
ATTCGCAGTT
AATCCTGGCC
TGTTAGAAAC
ATCAGAAGGC
TGTAGACAAA
TACTGGGACA
GCTACAACCA
TCCCTTCAGA
CAGGATCAGA
AGAACTTAGA
TCATTATATA
ATACAGTAGC
AACCCTCTAT
TGTGTGCATC
AAAGGATAGA
GATAAAAGAC
ACCAAGGAAG
CTTTAGACAA
GATAGAGGAA
GAGCAAAACA
AAAGTAAGAC
CACCGCACAG
CAAGCGGCCG
CTGATCTTCA
GACCTGGAGG
AGGAGATATG
AGGGACAATT
GGAGAAGTGA
ATTATATAAA
TATAAAGTAG
TAAAAATTGA
ACCATTAGGA
GTAGCACCCA
CCAAGGCAAA
GAGAAGAGTG
GTGCAGAGAG
AAAAAAGAGC
AGTGGGAATA
GGAGCTTTGT
TCCTTGGGTT
CTTGGGAGCA
GCAGGAAGCA
CTATGGGCGC
AGCGTCAATG
ACGCTGACGG
TACAGGCCAG
ACAATTATTG
TCTGGTATAG
TGCAGCAGCA
GAACAATTTG
CTGAGGGCTA
TTGAGGCGCA
ACAGCATCTG
TTGCAACTCA
CAGTCTGGGG
CATCAAGCAG
CTCCAGGCAA
GAATCCTGGC
TGTGGAAAGA
TACCTAAAGG
ATCAACAGCT
CCTGGGGATT
TGGGGTTGCT
CTGGAAAACT
CATTTGCACC
ACTGCTGTGC
CTTGGAATGC
TAGTTGGAGT
AATAAATCTC
TGGAACAGAT
TTGGAATCAC
ACGACCTGGA
TGGAGTGGGA
CAGAGAAATT
AACAATTACA
CAAGCTTAAT
ACACTCCTTA
ATTGAAGAAT
CGCAAAACCA
GCAAGAAAAG
AATGAACAAG
AATTATTGGA
ATTAGATAAA
TGGGCAAGTT
TGTGGAATTG
GTTTAACATA
ACAAATTGGC
TGTGGTATAT
AAAATTATTC
ATAATGATAG
TAGGAGGCTT
GGTAGGTTTA
AGAATAGTTT
TTGCTGTACT
TTCTATAGTG
AATAGAGTTA
GGCAGGGATA
TTCACCATTA
TCGTTTCAGA
CCCACCTCCC
AACCCCGAGG
GGACCCGACA
GGCCCGAAGG
AATAGAAGAA
GAAGGTGGAG
AGAGAGACAG
AGACAGATCC
ATTCGATTAG
TGAACGGATC
GGCACTGCGT
GCGCCAATTC
TGCAGACAAA
TGGCAGTATT
CATCCACAAT
TTTAAAAGAA
AAGGGGGGAT
TGGGGGGTAC
AGTGCAGGGG
AAAGAATAGT
AGACATAATA
GCAACAGACA
TACAAACTAA
AGAATTACAA
AAACAAATTA
CAAAAATTCA
AAATTTTCGG
GTTTATTACA
GGGACAGCAG
AGATCCAGTT
TGGTTAATTA
AGGTACCGAG
GGCCTATTTC
CCATGATTCC
TTCATATTTG
CATATACGAT
ACAAGGCTGT
TAGAGAGATA
ATTAGAATTA
ATTTGACTGT
AAACACAAAG
ATATTAGTAC
AAAATACGTG
ACGTAGAAAG
TAATAATTTC
TTGGGTAGTT
TGCAGTTTTA
AAATTATGTT
TTAAAATGGA
CTATCATATG
CTTACCGTAA
CTTGAAAGTA
TTTCGATTTC
TTGGCTTTAT
ATATCTTGTG
GAAAGGACGA
AACACCGGGT
ATGTGGATTC
CACCCAGAGG
GTTTTAGAGC
TAGAAATAGC
AAGTTAAAAT
AAGGCTAGTC
CGTTATCAAC
TTGAAAAAGT
GGCACCGAGT
CGGTGCTTTT
TTGAATTCGC
TAGCTAGGTC
TTGAAAGGAG
TGGGAATTGG
CTCCGGTGCC
CGTCAGTGGG
CAGAGCGCAC
ATCGCCCACA
GTCCCCGAGA
AGTTGGGGGG
AGGGGTCGGC
AATTGATCCG
GTGCCTAGAG
AAGGTGGCGC
GGGGTAAACT
GGGAAAGTGA
TGTCGTGTAC
TGGCTCCGCC
TTTTTCCCGA
GGGTGGGGGA
GAACCGTATA
TAAGTGCAGT
AGTCGCCGTG
AACGTTCTTT
TTCGCAACGG
GTTTGCCGCC
AGAACACAGG
ACCGGTTCTA
GAGCGCTGCC
ACCATGGACA
AGAAGTACAG
CATCGGCCTG
GACATCGGCA
CCAACTCTGT
GGGCTGGGCC
GTGATCACCG
ACGAGTACAA
GGTGCCCAGC
AAGAAATTCA
AGGTGCTGGG
CAACACCGAC
CGGCACAGCA
TCAAGAAGAA
CCTGATCGGA
GCCCTGCTGT
TCGACAGCGG
CGAAACAGCC
GAGGCCACCC
GGCTGAAGAG
AACCGCCAGA
AGAAGATACA
CCAGACGGAA
GAACCGGATC
TGCTATCTGC
AAGAGATCTT
CAGCAACGAG
ATGGCCAAGG
TGGACGACAG
CTTCTTCCAC
AGACTGGAAG
AGTCCTTCCT
GGTGGAAGAG
GATAAGAAGC
ACGAGCGGCA
CCCCATCTTC
GGCAACATCG
TGGACGAGGT
GGCCTACCAC
GAGAAGTACC
CCACCATCTA
CCACCTGAGA
AAGAAACTGG
TGGACAGCAC
CGACAAGGCC
GACCTGCGGC
TGATCTATCT
GGCCCTGGCC
CACATGATCA
AGTTCCGGGG
CCACTTCCTG
ATCGAGGGCG
ACCTGAACCC
CGACAACAGC
GACGTGGACA
AGCTGTTCAT
CCAGCTGGTG
CAGACCTACA
ACCAGCTGTT
CGAGGAAAAC
CCCATCAACG
CCAGCGGCGT
GGACGCCAAG
GCCATCCTGT
CTGCCAGACT
GAGCAAGAGC
AGACGGCTGG
AAAATCTGAT
CGCCCAGCTG
CCCGGCGAGA
AGAAGAATGG
CCTGTTCGGA
AACCTGATTG
CCCTGAGCCT
GGGCCTGACC
CCCAACTTCA
AGAGCAACTT
CGACCTGGCC
GAGGATGCCA
AACTGCAGCT
GAGCAAGGAC
ACCTACGACG
ACGACCTGGA
CAACCTGCTG
GCCCAGATCG
GCGACCAGTA
CGCCGACCTG
TTTCTGGCCG
CCAAGAACCT
GTCCGACGCC
ATCCTGCTGA
GCGACATCCT
GAGAGTGAAC
ACCGAGATCA
CCAAGGCCCC
CCTGAGCGCC
TCTATGATCA
AGAGATACGA
CGAGCACCAC
CAGGACCTGA
CCCTGCTGAA
AGCTCTCGTG
CGGCAGCAGC
TGCCTGAGAA
GTACAAAGAG
ATTTTCTTCG
ACCAGAGCAA
GAACGGCTAC
GCCGGCTACA
TTGACGGCGG
AGCCAGCCAG
GAAGAGTTCT
ACAAGTTCAT
CAAGCCCATC
CTGGAAAAGA
TGGACGGCAC
CGAGGAACTG
CTCGTGAAGC
TGAACAGAGA
GGACCTGCTG
CGGAAGCAGC
GGACCTTCGA
CAACGGCAGC
ATCCCCCACC
AGATCCACCT
GGGAGAGCTG
CACGCCATTC
TGCGGCGGCA
GGAAGATTTT
TACCCATTCC
TGAAGGACAA
CCGGGAAAAG
ATCGAGAAGA
TCCTGACCTT
CCGCATCCCC
TACTACGTGG
GCCCTCTGGC
CAGGGGAAAC
AGCAGATTCG
CCTGGATGAC
CAGAAAGAGC
GAGGAAACCA
TCACCCCCTG
GAACTTCGAG
GAAGTGGTGG
ACAAGGGCGC
TTCCGCCCAG
AGCTTCATCG
AGCGGATGAC
CAACTTCGAT
AAGAACCTGC
CCAACGAGAA
GGTGCTGCCC
AAGCACAGCC
TGCTGTACGA
GTACTTCACC
GTGTATAACG
AGCTGACCAA
AGTGAAATAC
GTGACCGAGG
GAATGAGAAA
GCCCGCCTTC
CTGAGCGGCG
AGCAGAAAAA
GGCCATCGTG
GACCTGCTGT
TCAAGACCAA
CCGGAAAGTG
ACCGTGAAGC
AGCTGAAAGA
GGACTACTTC
AAGAAAATCG
AGTGCTTCGA
CTCCGTGGAA
ATCTCCGGCG
TGGAAGATCG
GTTCAACGCC
TCCCTGGGCA
CATACCACGA
TCTGCTGAAA
ATTATCAAGG
ACAAGGACTT
CCTGGACAAT
GAGGAAAACG
AGGACATTCT
GGAAGATATC
GTGCTGACCC
TGACACTGTT
TGAGGACAGA
GAGATGATCG
AGGAACGGCT
GAAAACCTAT
GCCCACCTGT
TCGACGACAA
AGTGATGAAG
CAGCTGAAGC
GGCGGAGATA
CACCGGCTGG
GGCAGGCTGA
GCCGGAAGCT
GATCAACGGC
ATCCGGGACA
AGCAGTCCGG
CAAGACAATC
CTGGATTTCC
TGAAGTCCGA
CGGCTTCGCC
AACAGAAACT
TCATGCAGCT
GATCCACGAC
GACAGCCTGA
CCTTTAAAGA
GGACATCCAG
AAAGCCCAGG
TGTCCGGCCA
GGGCGATAGC
CTGCACGAGC
ACATTGCCAA
TCTGGCCGGC
AGCCCCGCCA
TTAAGAAGGG
CATCCTGCAG
ACAGTGAAGG
TGGTGGACGA
GCTCGTGAAA
GTGATGGGCC
GGCACAAGCC
CGAGAACATC
GTGATCGAAA
TGGCCAGAGA
GAACCAGACC
ACCCAGAAGG
GACAGAAGAA
CAGCCGCGAG
AGAATGAAGC
GGATCGAAGA
GGGCATCAAA
GAGCTGGGCA
GCCAGATCCT
GAAAGAACAC
CCCGTGGAAA
ACACCCAGCT
GCAGAACGAG
AAGCTGTACC
TGTACTACCT
GCAGAATGGG
CGGGATATGT
ACGTGGACCA
GGAACTGGAC
ATCAACCGGC
TGTCCGACTA
CGATGTGGAC
CATATCGTGC
CTCAGAGCTT
TCTGAAGGAC
GACTCCATCG
ACAACAAGGT
GCTGACCAGA
AGCGACAAGA
ACCGGGGCAA
GAGCGACAAC
GTGCCCTCCG
AAGAGGTCGT
GAAGAAGATG
AAGAACTACT
GGCGGCAGCT
GCTGAACGCC
AAGCTGATTA
CCCAGAGAAA
GTTCGACAAT
CTGACCAAGG
CCGAGAGAGG
CGGCCTGAGC
GAACTGGATA
AGGCCGGCTT
CATCAAGAGA
CAGCTGGTGG
AAACCCGGCA
GATCACAAAG
CACGTGGCAC
AGATCCTGGA
CTCCCGGATG
AACACTAAGT
ACGACGAGAA
TGACAAGCTG
ATCCGGGAAG
TGAAAGTGAT
CACCCTGAAG
TCCAAGCTGG
TGTCCGATTT
CCGGAAGGAT
TTCCAGTTTT
ACAAAGTGCG
CGAGATCAAC
AACTACCACC
ACGCCCACGA
CGCCTACCTG
AACGCCGTCG
TGGGAACCGC
CCTGATCAAA
AAGTACCCTA
AGCTGGAAAG
CGAGTTCGTG
TACGGCGACT
ACAAGGTGTA
CGACGTGCGG
AAGATGATCG
CCAAGAGCGA
GCAGGAAATC
GGCAAGGCTA
CCGCCAAGTA
CTTCTTCTAC
AGCAACATCA
TGAACTTTTT
CAAGACCGAG
ATTACCCTGG
CCAACGGCGA
GATCCGGAAG
CGGCCTCTGA
TCGAGACAAA
CGGCGAAACC
GGGGAGATCG
TGTGGGATAA
GGGCCGGGAT
TTTGCCACCG
TGCGGAAAGT
GCTGAGCATG
CCCCAAGTGA
ATATCGTGAA
AAAGACCGAG
GTGCAGACAG
GCGGCTTCAG
CAAAGAGTCT
ATCCTGCCCA
AGAGGAACAG
CGATAAGCTG
ATCGCCAGAA
AGAAGGACTG
GGACCCTAAG
AAGTACGGCG
GCTTCGACAG
CCCCACCGTG
GCCTATTCTG
TGCTGGTGGT
GGCCAAAGTG
GAAAAGGGCA
AGTCCAAGAA
ACTGAAGAGT
GTGAAAGAGC
TGCTGGGGAT
CACCATCATG
GAAAGAAGCA
GCTTCGAGAA
GAATCCCATC
GACTTTCTGG
AAGCCAAGGG
CTACAAAGAA
GTGAAAAAGG
ACCTGATCAT
CAAGCTGCCT
AAGTACTCCC
TGTTCGAGCT
GGAAAACGGC
CGGAAGAGAA
TGCTGGCCTC
TGCCGGCGAA
CTGCAGAAGG
GAAACGAACT
GGCCCTGCCC
TCCAAATATG
TGAACTTCCT
GTACCTGGCC
AGCCACTATG
AGAAGCTGAA
GGGCTCCCCC
GAGGATAATG
AGCAGAAACA
GCTGTTTGTG
GAACAGCACA
AGCACTACCT
GGACGAGATC
ATCGAGCAGA
TCAGCGAGTT
CTCCAAGAGA
GTGATCCTGG
CCGACGCTAA
TCTGGACAAA
GTGCTGTCCG
CCTACAACAA
GCACCGGGAT
AAGCCCATCA
GAGAGCAGGC
CGAGAATATC
ATCCACCTGT
TTACCCTGAC
CAATCTGGGA
GCCCCTGCCG
CCTTCAAGTA
CTTTGACACC
ACCATCGACC
GGAAGAGGTA
CACCAGCACC
AAAGAGGTGC
TGGACGCCAC
CCTGATCCAC
CAGAGCATCA
CCGGCCTGTA
CGAGACACGG
ATCGACCTGT
CTCAGCTGGG
AGGCGACAAG
CGACCTGCCG
CCACAAAGAA
GGCTGGACAG
GCTAAGAAGA
AGAAAGATTA
CAAAGACGAT
GACGATAAGG
GATCCGGCGC
AACAAACTTC
TCTCTGCTGA
AACAAGCCGG
AGATGTCGAA
GAGAATCCTG
GACCGACCGA
GTACAAGCCC
ACGGTGCGCC
TCGCCACCCG
CGACGACGTC
CCCAGGGCCG
TACGCACCCT
CGCCGCCGCG
TTCGCCGACT
ACCCCGCCAC
GCGCCACACC
GTCGATCCGG
ACCGCCACAT
CGAGCGGGTC
ACCGAGCTGC
AAGAACTCTT
CCTCACGCGC
GTCGGGCTCG
ACATCGGCAA
GGTGTGGGTC
GCGGACGACG
GCGCCGCGGT
GGCGGTCTGG
ACCACGCCGG
AGAGCGTCGA
AGCGGGGGCG
GTGTTCGCCG
AGATCGGCCC
GCGCATGGCC
GAGTTGAGCG
GTTCCCGGCT
GGCCGCGCAG
CAACAGATGG
AAGGCCTCCT
GGCGCCGCAC
CGGCCCAAGG
AGCCCGCGTG
GTTCCTGGCC
ACCGTCGGAG
TCTCGCCCGA
CCACCAGGGC
AAGGGTCTGG
GCAGCGCCGT
CGTGCTCCCC
GGAGTGGAGG
CGGCCGAGCG
CGCCGGGGTG
CCCGCCTTCC
TGGAGACCTC
CGCGCCCCGC
AACCTCCCCT
TCTACGAGCG
GCTCGGCTTC
ACCGTCACCG
CCGACGTCGA
GGTGCCCGAA
GGACCGCGCA
CCTGGTGCAT
GACCCGCAAG
CCCGGTGCCT
GAACGCGTTA
AGTCGACAAT
CAACCTCTGG
ATTACAAAAT
TTGTGAAAGA
TTGACTGGTA
TTCTTAACTA
TGTTGCTCCT
TTTACGCTAT
GTGGATACGC
TGCTTTAATG
CCTTTGTATC
ATGCTATTGC
TTCCCGTATG
GCTTTCATTT
TCTCCTCCTT
GTATAAATCC
TGGTTGCTGT
CTCTTTATGA
GGAGTTGTGG
CCCGTTGTCA
GGCAACGTGG
CGTGGTGTGC
ACTGTGTTTG
CTGACGCAAC
CCCCACTGGT
TGGGGCATTG
CCACCACCTG
TCAGCTCCTT
TCCGGGACTT
TCGCTTTCCC
CCTCCCTATT
GCCACGGCGG
AACTCATCGC
CGCCTGCCTT
GCCCGCTGCT
GGACAGGGGC
TCGGCTGTTG
GGCACTGACA
ATTCCGTGGT
GTTGTCGGGG
AAATCATCGT
CCTTTCCTTG
GCTGCTCGCC
TGTGTTGCCA
CCTGGATTCT
GCGCGGGACG
TCCTTCTGCT
ACGTCCCTTC
GGCCCTCAAT
CCAGCGGACC
TTCCTTCCCG
CGGCCTGCTG
CCGGCTCTGC
GGCCTCTTCC
GCGTCTTCGC
CTTCGCCCTC
AGACGAGTCG
GATCTCCCTT
TGGGCCGCCT
CCCCGCGTCG
ACTTTAAGAC
CAATGACTTA
CAAGGCAGCT
GTAGATCTTA
GCCACTTTTT
AAAAGAAAAG
GGGGGACTGG
AAGGGCTAAT
TCACTCCCAA
CGAAGACAAG
ATCTGCTTTT
TGCTTGTACT
GGGTCTCTCT
GGTTAGACCA
GATCTGAGCC
TGGGAGCTCT
CTGGCTAACT
AGGGAACCCA
CTGCTTAAGC
CTCAATAAAG
CTTGCCTTGA
GTGCTTCAAG
TAGTGTGTGC
CCGTCTGTTG
TGTGACTCTG
GTAACTAGAG
ATCCCTCAGA
CCCTTTTAGT
CAGTGTGGAA
AATCTCTAGC
AGGGCCCGTT
TAAACCCGCT
GATCAGCCTC
GACTGTGCCT
TCTAGTTGCC
AGCCATCTGT
TGTTTGCCCC
TCCCCCGTGC
CTTCCTTGAC
CCTGGAAGGT
GCCACTCCCA
CTGTCCTTTC
CTAATAAAAT
GAGGAAATTG
CATCGCATTG
TCTGAGTAGG
TGTCATTCTA
TTCTGGGGGG
TGGGGTGGGG
CAGGACAGCA
AGGGGGAGGA
TTGGGAAGAC
AATAGCAGGC
ATGCTGGGGA
TGCGGTGGGC
TCTATGGCTT
CTGAGGCGGA
AAGAACCAGC
TGGGGCTCTA
GGGGGTATCC
CCACGCGCCC
TGTAGCGGCG
CATTAAGCGC
GGCGGGTGTG
GTGGTTACGC
GCAGCGTGAC
CGCTACACTT
GCCAGCGCCC
TAGCGCCCGC
TCCTTTCGCT
TTCTTCCCTT
CCTTTCTCGC
CACGTTCGCC
GGCTTTCCCC
GTCAAGCTCT
AAATCGGGGG
CTCCCTTTAG
GGTTCCGATT
TAGTGCTTTA
CGGCACCTCG
ACCCCAAAAA
ACTTGATTAG
GGTGATGGTT
CACGTAGTGG
GCCATCGCCC
TGATAGACGG
TTTTTCGCCC
TTTGACGTTG
GAGTCCACGT
TCTTTAATAG
TGGACTCTTG
TTCCAAACTG
GAACAACACT
CAACCCTATC
TCGGTCTATT
CTTTTGATTT
ATAAGGGATT
TTGCCGATTT
CGGCCTATTG
GTTAAAAAAT
GAGCTGATTT
AACAAAAATT
TAACGCGAAT
TAATTCTGTG
GAATGTGTGT
CAGTTAGGGT
GTGGAAAGTC
CCCAGGCTCC
CCAGCAGGCA
GAAGTATGCA
AAGCATGCAT
CTCAATTAGT
CAGCAACCAG
GTGTGGAAAG
TCCCCAGGCT
CCCCAGCAGG
CAGAAGTATG
CAAAGCATGC
ATCTCAATTA
GTCAGCAACC
ATAGTCCCGC
CCCTAACTCC
GCCCATCCCG
CCCCTAACTC
CGCCCAGTTC
CGCCCATTCT
CCGCCCCATG
GCTGACTAAT
TTTTTTTATT
TATGCAGAGG
CCGAGGCCGC
CTCTGCCTCT
GAGCTATTCC
AGAAGTAGTG
AGGAGGCTTT
TTTGGAGGCC
TAGGCTTTTG
CAAAAAGCTC
CCGGGAGCTT
GTATATCCAT
TTTCGGATCT
GATCAGCACG
TGTTGACAAT
TAATCATCGG
CATAGTATAT
CGGCATAGTA
TAATACGACA
AGGTGAGGAA
CTAAACCATG
GCCAAGTTGA
CCAGTGCCGT
TCCGGTGCTC
ACCGCGCGCG
ACGTCGCCGG
AGCGGTCGAG
TTCTGGACCG
ACCGGCTCGG
GTTCTCCCGG
GACTTCGTGG
AGGACGACTT
CGCCGGTGTG
GTCCGGGACG
ACGTGACCCT
GTTCATCAGC
GCGGTCCAGG
ACCAGGTGGT
GCCGGACAAC
ACCCTGGCCT
GGGTGTGGGT
GCGCGGCCTG
GACGAGCTGT
ACGCCGAGTG
GTCGGAGGTC
GTGTCCACGA
ACTTCCGGGA
CGCCTCCGGG
CCGGCCATGA
CCGAGATCGG
CGAGCAGCCG
TGGGGGCGGG
AGTTCGCCCT
GCGCGACCCG
GCCGGCAACT
GCGTGCACTT
CGTGGCCGAG
GAGCAGGACT
GACACGTGCT
ACGAGATTTC
GATTCCACCG
CCGCCTTCTA
TGAAAGGTTG
GGCTTCGGAA
TCGTTTTCCG
GGACGCCGGC
TGGATGATCC
TCCAGCGCGG
GGATCTCATG
CTGGAGTTCT
TCGCCCACCC
CAACTTGTTT
ATTGCAGCTT
ATAATGGTTA
CAAATAAAGC
AATAGCATCA
CAAATTTCAC
AAATAAAGCA
TTTTTTTCAC
TGCATTCTAG
TTGTGGTTTG
TCCAAACTCA
TCAATGTATC
TTATCATGTC
TGTATACCGT
CGACCTCTAG
CTAGAGCTTG
GCGTAATCAT
GGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGAACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGAGACCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATTGCTAC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAT
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATACTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTTCCGCGCA
CATTTCCCCG
AAAAGTGCCA
CCTGAC