名称: | Ef1a_Large+T-antigen_Ires_mCherry |
---|---|
别称: | |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达,永生化 |
片段类型: | ORF |
片段物种: | 病毒 |
原核抗性: | Amp |
筛选标记: | Bleo |
荧光标记: | 红色 |
启动子: | EF-1a |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | Ef1a_Large T-antigen_Ires_PuroT |
正向引物: | BGH |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TTACCAATGC
TTAATCAGTG
AGGCACCTAT
CTCAGCGATC
TGTCTATTTC
GTTCATCCAT
AGTTGCCTGA
CTCCCCGTCG
TGTAGATAAC
TACGATACGG
GAGGGCTTAC
CATCTGGCCC
CAGTGCTGCA
ATGATACCGC
GAGACCCACG
CTCACCGGCT
CCAGATTTAT
CAGCAATAAA
CCAGCCAGCC
GGAAGGGCCG
AGCGCAGAAG
TGGTCCTGCA
ACTTTATCCG
CCTCCATCCA
GTCTATTAAT
TGTTGCCGGG
AAGCTAGAGT
AAGTAGTTCG
CCAGTTAATA
GTTTGCGCAA
CGTTGTTGCC
ATTGCTACAG
GCATCGTGGT
GTCACGCTCG
TCGTTTGGTA
TGGCTTCATT
CAGCTCCGGT
TCCCAACGAT
CAAGGCGAGT
TACATGATCC
CCCATGTTGT
GCAAAAAAGC
GGTTAGCTCC
TTCGGTCCTC
CGATCGTTGT
CAGAAGTAAG
TTGGCCGCAG
TGTTATCACT
CATGGTTATG
GCAGCACTGC
ATAATTCTCT
TACTGTCATG
CCATCCGTAA
GATGCTTTTC
TGTGACTGGT
GAGTACTCAA
CCAAGTCATT
CTGAGAATAG
TGTATGCGGC
GACCGAGTTG
CTCTTGCCCG
GCGTCAATAC
GGGATAATAC
CGCGCCACAT
AGCAGAACTT
TAAAAGTGCT
CATCATTGGA
AAACGTTCTT
CGGGGCGAAA
ACTCTCAAGG
ATCTTACCGC
TGTTGAGATC
CAGTTCGATG
TAACCCACTC
GTGCACCCAA
CTGATCTTCA
GCATCTTTTA
CTTTCACCAG
CGTTTCTGGG
TGAGCAAAAA
CAGGAAGGCA
AAATGCCGCA
AAAAAGGGAA
TAAGGGCGAC
ACGGAAATGT
TGAATACTCA
TACTCTTCCT
TTTTCAATAT
TATTGAAGCA
TTTATCAGGG
TTATTGTCTC
ATGAGCGGAT
ACATATTTGA
ATGTATTTAG
AAAAATAAAC
AAATAGGGGT
TCCGCGCACA
TTTCCCCGAA
AAGTGCCACC
TGACGTCGAC
GGATCGGGAG
ATCTCCCGAT
CCCCTATGGT
GCACTCTCAG
TACAATCTGC
TCTGATGCCG
CATAGTTAAG
CCAGTATCTG
CTCCCTGCTT
GTGTGTTGGA
GGTCGCTGAG
TAGTGCGCGA
GCAAAATTTA
AGCTACAACA
AGGCAAGGCT
TGACCGACAA
TTGCATGAAG
AATCTGCTTA
GGGTTAGGCG
TTTTGCGCTG
CTTCGCGATG
TACGGGCCAG
ATATACGCGT
TGACATTGAT
TATTGACTAG
TTATTAATAG
TAATCAATTA
CGGGGTCATT
AGTTCATAGC
CCATATATGG
AGTTCCGCGT
TACATAACTT
ACGGTAAATG
GCCCGCCTGG
CTGACCGCCC
AACGACCCCC
GCCCATTGAC
GTCAATAATG
ACGTATGTTC
CCATAGTAAC
GCCAATAGGG
ACTTTCCATT
GACGTCAATG
GGTGGAGTAT
TTACGGTAAA
CTGCCCACTT
GGCAGTACAT
CAAGTGTATC
ATATGCCAAG
TACGCCCCCT
ATTGACGTCA
ATGACGGTAA
ATGGCCCGCC
TGGCATTATG
CCCAGTACAT
GACCTTATGG
GACTTTCCTA
CTTGGCAGTA
CATCTACGTA
TTAGTCATCG
CTATTACCAT
GGTGATGCGG
TTTTGGCAGT
ACATCAATGG
GCGTGGATAG
CGGTTTGACT
CACGGGGATT
TCCAAGTCTC
CACCCCATTG
ACGTCAATGG
GAGTTTGTTT
TGGCACCAAA
ATCAACGGGA
CTTTCCAAAA
TGTCGTAACA
ACTCCGCCCC
ATTGACGCAA
ATGGGCGGTA
GGCGTGTACG
GTGGGAGGTC
TATATAAGCA
GCGCGTTTTG
CCTGTACTGG
GTCTCTCTGG
TTAGACCAGA
TCTGAGCCTG
GGAGCTCTCT
GGCTAACTAG
GGAACCCACT
GCTTAAGCCT
CAATAAAGCT
TGCCTTGAGT
GCTTCAAGTA
GTGTGTGCCC
GTCTGTTGTG
TGACTCTGGT
AACTAGAGAT
CCCTCAGACC
CTTTTAGTCA
GTGTGGAAAA
TCTCTAGCAG
TGGCGCCCGA
ACAGGGACTT
GAAAGCGAAA
GGGAAACCAG
AGGAGCTCTC
TCGACGCAGG
ACTCGGCTTG
CTGAAGCGCG
CACGGCAAGA
GGCGAGGGGC
GGCGACTGGT
GAGTACGCCA
AAAATTTTGA
CTAGCGGAGG
CTAGAAGGAG
AGAGATGGGT
GCGAGAGCGT
CAGTATTAAG
CGGGGGAGAA
TTAGATCGCG
ATGGGAAAAA
ATTCGGTTAA
GGCCAGGGGG
AAAGAAAAAA
TATAAATTAA
AACATATAGT
ATGGGCAAGC
AGGGAGCTAG
AACGATTCGC
AGTTAATCCT
GGCCTGTTAG
AAACATCAGA
AGGCTGTAGA
CAAATACTGG
GACAGCTACA
ACCATCCCTT
CAGACAGGAT
CAGAAGAACT
TAGATCATTA
TATAATACAG
TAGCAACCCT
CTATTGTGTG
CATCAAAGGA
TAGAGATAAA
AGACACCAAG
GAAGCTTTAG
ACAAGATAGA
GGAAGAGCAA
AACAAAAGTA
AGACCACCGC
ACAGCAAGCG
GCCGCTGATC
TTCAGACCTG
GAGGAGGAGA
TATGAGGGAC
AATTGGAGAA
GTGAATTATA
TAAATATAAA
GTAGTAAAAA
TTGAACCATT
AGGAGTAGCA
CCCACCAAGG
CAAAGAGAAG
AGTGGTGCAG
AGAGAAAAAA
GAGCAGTGGG
AATAGGAGCT
TTGTTCCTTG
GGTTCTTGGG
AGCAGCAGGA
AGCACTATGG
GCGCAGCGTC
AATGACGCTG
ACGGTACAGG
CCAGACAATT
ATTGTCTGGT
ATAGTGCAGC
AGCAGAACAA
TTTGCTGAGG
GCTATTGAGG
CGCAACAGCA
TCTGTTGCAA
CTCACAGTCT
GGGGCATCAA
GCAGCTCCAG
GCAAGAATCC
TGGCTGTGGA
AAGATACCTA
AAGGATCAAC
AGCTCCTGGG
GATTTGGGGT
TGCTCTGGAA
AACTCATTTG
CACCACTGCT
GTGCCTTGGA
ATGCTAGTTG
GAGTAATAAA
TCTCTGGAAC
AGATTTGGAA
TCACACGACC
TGGATGGAGT
GGGACAGAGA
AATTAACAAT
TACACAAGCT
TAATACACTC
CTTAATTGAA
GAATCGCAAA
ACCAGCAAGA
AAAGAATGAA
CAAGAATTAT
TGGAATTAGA
TAAATGGGCA
AGTTTGTGGA
ATTGGTTTAA
CATAACAAAT
TGGCTGTGGT
ATATAAAATT
ATTCATAATG
ATAGTAGGAG
GCTTGGTAGG
TTTAAGAATA
GTTTTTGCTG
TACTTTCTAT
AGTGAATAGA
GTTAGGCAGG
GATATTCACC
ATTATCGTTT
CAGACCCACC
TCCCAACCCC
GAGGGGACCC
GACAGGCCCG
AAGGAATAGA
AGAAGAAGGT
GGAGAGAGAG
ACAGAGACAG
ATCCATTCGA
TTAGTGAACG
GATCGGCACT
GCGTGCGCCA
ATTCTGCAGA
CAAATGGCAG
TATTCATCCA
CAATTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TCGGGTTTAT
TACAGGGACA
GCAGAGATCC
AGTTTGGTTA
ATTAATCGAT
AAGCTTTGCA
AAGATGGATA
AAGTTTTAAA
CAGAGAGGAA
TCTTTGCAGC
TAATGGACCT
TCTAGGTCTT
GAAAGGAGTG
GGAATTGGCT
CCGGTGCCCG
TCAGTGGGCA
GAGCGCACAT
CGCCCACAGT
CCCCGAGAAG
TTGGGGGGAG
GGGTCGGCAA
TTGAACCGGT
GCCTAGAGAA
GGTGGCGCGG
GGTAAACTGG
GAAAGTGATG
TCGTGTACTG
GCTCCGCCTT
TTTCCCGAGG
GTGGGGGAGA
ACCGTATATA
AGTGCAGTAG
TCGCCGTGAA
CGTTCTTTTT
CGCAACGGGT
TTGCCGCCAG
AACACAGGTA
AGTGCCGTGT
GTGGTTCCCG
CGGGCCTGGC
CTCTTTACGG
GTTATGGCCC
TTGCGTGCCT
TGAATTACTT
CCACCTGGCT
GCAGTACGTG
ATTCTTGATC
CCGAGCTTCG
GGTTGGAAGT
GGGTGGGAGA
GTTCGAGGCC
TTGCGCTTAA
GGAGCCCCTT
CGCCTCGTGC
TTGAGTTGAG
GCCTGGCCTG
GGCGCTGGGG
CCGCCGCGTG
CGAATCTGGT
GGCACCTTCG
CGCCTGTCTC
GCTGCTTTCG
ATAAGTCTCT
AGCCATTTAA
AATTTTTGAT
GACCTGCTGC
GACGCTTTTT
TTCTGGCAAG
ATAGTCTTGT
AAATGCGGGC
CAAGATCTGC
ACACTGGTAT
TTCGGTTTTT
GGGGCCGCGG
GCGGCGACGG
GGCCCGTGCG
TCCCAGCGCA
CATGTTCGGC
GAGGCGGGGC
CTGCGAGCGC
GGCCACCGAG
AATCGGACGG
GGGTAGTCTC
AAGCTGGCCG
GCCTGCTCTG
GTGCCTGGCC
TCGCGCCGCC
GTGTATCGCC
CCGCCCTGGG
CGGCAAGGCT
GGCCCGGTCG
GCACCAGTTG
CGTGAGCGGA
AAGATGGCCG
CTTCCCGGCC
CTGCTGCAGG
GAGCTCAAAA
TGGAGGACGC
GGCGCTCGGG
AGAGCGGGCG
GGTGAGTCAC
CCACACAAAG
GAAAAGGGCC
TTTCCGTCCT
CAGCCGTCGC
TTCATGTGAC
TCCACGGAGT
ACCGGGCGCC
GTCCAGGCAC
CTCGATTAGT
TCTCGAGCTT
TTGGAGTACG
TCGTCTTTAG
GTTGGGGGGA
GGGGTTTTAT
GCGATGGAGT
TTCCCCACAC
TGAGTGGGTG
GAGACTGAAG
TTAGGCCAGC
TTGGCACTTG
ATGTAATTCT
CCTTGGAATT
TGCCCTTTTT
GAGTTTGGAT
CTTGGTTCAT
TCTCAAGCCT
CAGACAGTGG
TTCAAAGTTT
TTTTCTTCCA
TTTCAGGTGT
CGTGAGGAAT
TAGCTTGGTA
CTAATACGAC
TCACTATAGG
GAGACCCAAG
CTGGCTAGGT
AAGCTTGATA
TCGAATTCGC
CCTTATCGTC
TAGAATGGAT
AAAGTTTTAA
ACAGAGAGGA
ATCTTTGCAG
CTAATGGACC
TTCTAGGTCT
TGAAAGGAGT
GCCTGGGGGA
ATATTCCTCT
GATGAGAAAG
GCATATTTAA
AAAAATGCAA
GGAGTTTCAT
CCTGATAAAG
GAGGAGATGA
AGAAAAAATG
AAGAAAATGA
ATACTCTGTA
CAAGAAAATG
GAAGATGGAG
TAAAATATGC
TCATCAACCT
GACTTTGGAG
GCTTCTGGGA
TGCAACTGAG
ATTCCAACCT
ATGGAACTGA
TGAATGGGAG
CAGTGGTGGA
ATGCCTTTAA
TGAGGAAAAC
CTGTTTTGCT
CAGAAGAAAT
GCCATCTAGT
GATGATGAGG
CTACTGCTGA
CTCTCAACAT
TCTACTCCTC
CAAAAAAGAA
GAGAAAGGTA
GAAGACCCCA
AGGACTTTCC
TTCAGAATTG
CTAAGTTTTT
TGAGTCATGC
TGTGTTTAGT
AATAGAACTC
TTGCTTGCTT
TGCTATTTAC
ACCACAAAGG
AAAAAGCTGC
ACTGCTATAC
AAGAAAATTA
TGGAAAAATA
TTCTGTAACC
TTTATAAGTA
GGCATAACAG
TTATAATCAT
AACATACTGT
TTTTTCTTAC
TCCACACAGG
CATAGAGTGT
CTGCTATTAA
TAACTATGCT
CAAAAATTGT
GTACCTTTAG
CTTTTTAATT
TGTAAAGGGG
TTAATAAGGA
ATATTTGATG
TATAGTGCCT
TGACTAGAGA
TCCATTTTCT
GTTATTGAGG
AAAGTTTGCC
AGGTGGGTTA
AAGGAGCATG
ATTTTAATCC
AGAAGAAGCA
GAGGAAACTA
AACAAGTGTC
CTGGAAGCTT
GTAACAGAGT
ATGCAATGGA
AACAAAATGT
GATGATGTGT
TGTTATTGCT
TGGGATGTAC
TTGGAATTTC
AGTACAGTTT
TGAAATGTGT
TTAAAATGTA
TTAAAAAAGA
ACAGCCCAGC
CACTATAAGT
ACCATGAAAA
GCATTATGCA
AATGCTGCTA
TATTTGCTGA
CAGCAAAAAC
CAAAAAACCA
TATGCCAACA
GGCTGTTGAT
ACTGTTTTAG
CTAAAAAGCG
GGTTGATAGC
CTACAATTAA
CTAGAGAACA
AATGTTAACA
AACAGATTTA
ATGATCTTTT
GGATAGGATG
GATATAATGT
TTGGTTCTAC
AGGCTCTGCT
GACATAGAAG
AATGGATGGC
TGGAGTTGCT
TGGCTACACT
GTTTGTTGCC
CAAAATGGAT
TCAGTGGTGT
ATGACTTTTT
AAAATGCATG
GTGTACAACA
TTCCTAAAAA
AAGATACTGG
CTGTTTAAAG
GACCAATTGA
TAGTGGTAAA
ACTACATTAG
CAGCTGCTTT
GCTTGAATTA
TGTGGGGGGA
AAGCTTTAAA
TGTTAATTTG
CCCTTGGACA
GGCTGAACTT
TGAGCTAGGA
GTAGCTATTG
ACCAGTTTTT
AGTAGTTTTT
GAGGATGTAA
AGGGCACTGG
AGGGGAGTCC
AGAGATTTGC
CTTCAGGTCA
GGGAATTAAT
AACCTGGACA
ATTTAAGGGA
TTATTTGGAT
GGCAGTGTTA
AGGTAAACTT
AGAAAAGAAA
CACCTAAATA
AAAGAACTCA
AATATTTCCC
CCTGGAATAG
TCACCATGAA
TGAGTACAGT
GTGCCTAAAA
CACTGCAGGC
CAGATTTGTA
AAACAAATAG
ATTTTAGGCC
CAAAGATTAT
TTAAAGCATT
GCCTGGAACG
CAGTGAGTTT
TTGTTAGAAA
AGAGAATAAT
TCAAAGTGGC
ATTGCTTTGC
TTCTTATGTT
AATTTGGTAC
AGACCTGTGG
CTGAGTTTGC
TCAAAGTATT
CAGAGCAGAA
TTGTGGAGTG
GAAAGAGAGA
TTGGACAAAG
AGTTTAGTTT
GTCAGTGTAT
CAAAAAATGA
AGTTTAATGT
GGCTATGGGA
ATTGGAGTTT
TAGATTGGCT
AAGAAACAGT
GATGATGATG
ATGAAGACAG
CCAGGAAAAT
GCTGATAAAA
ATGAAGATGG
TGGGGAGAAG
AACATGGAAG
ACTCAGGGCA
TGAAACAGGC
ATTGATTCAC
AGTCCCAAGG
CTCATTTCAG
GCCCCTCAGT
CCTCACAGTC
TGTTCATGAT
CATAATCAGC
CATACCACAT
TTGTAGAGGT
TTTACTTGCT
TTAAAAAACC
TCCCACACCT
CCCCCTGAAC
CTGAAACATA
AGTTAACCGA
TAAGGGCGAA
TTCCGCCCCC
CCCCCCTCTC
CCTCCCCCCC
CCCTAACGTT
ACTGGCCGAA
GCCGCTTGGA
ATAAGGCCGG
TGTGCGTTTG
TCTATATGTT
ATTTTCCACC
ATATTGCCGT
CTTTTGGCAA
TGTGAGGGCC
CGGAAACCTG
GCCCTGTCTT
CTTGACGAGC
ATTCCTAGGG
GTCTTTCCCC
TCTCGCCAAA
GGAATGCAAG
GTCTGTTGAA
TGTCGTGAAG
GAAGCAGTTC
CTCTGGAAGC
TTCTTGAAGA
CAAACAACGT
CTGTAGCGAC
CCTTTGCAGG
CAGCGGAACC
CCCCACCTGG
CGACAGGTGC
CTCTGCGGCC
AAAAGCCACG
TGTATAAGAT
ACACCTGCAA
AGGCGGCACA
ACCCCAGTGC
CACGTTGTGA
GTTGGATAGT
TGTGGAAAGA
GTCAAATGGC
TCTCCTCAAG
CGTATTCAAC
AAGGGGCTGA
AGGATGCCCA
GAAGGTACCC
CATTGTATGG
GATCTGATCT
GGGGCCTCGG
TGCACATGCT
TTACATGTGT
TTAGTCGAGG
TTAAAAAAAC
GTCTAGGCCC
CCCGAACCAC
GGGGACGTGG
TTTTCCTTTG
AAAAACACGA
TGATAATATG
GCCACAACCA
TGGTGAGCAA
GGGCGAGGAG
GATAACATGG
CCATCATCAA
GGAGTTCATG
CGCTTCAAGG
TGCACATGGA
GGGCTCCGTG
AACGGCCACG
AGTTCGAGAT
CGAGGGCGAG
GGCGAGGGCC
GCCCCTACGA
GGGCACCCAG
ACCGCCAAGC
TGAAGGTGAC
CAAGGGTGGC
CCCCTGCCCT
TCGCCTGGGA
CATCCTGTCC
CCTCAGTTCA
TGTACGGCTC
CAAGGCCTAC
GTGAAGCACC
CCGCCGACAT
CCCCGACTAC
TTGAAGCTGT
CCTTCCCCGA
GGGCTTCAAG
TGGGAGCGCG
TGATGAACTT
CGAGGACGGC
GGCGTGGTGA
CCGTGACCCA
GGACTCCTCC
CTGCAGGACG
GCGAGTTCAT
CTACAAGGTG
AAGCTGCGCG
GCACCAACTT
CCCCTCCGAC
GGCCCCGTAA
TGCAGAAGAA
GACCATGGGC
TGGGAGGCCT
CCTCCGAGCG
GATGTACCCC
GAGGACGGCG
CCCTGAAGGG
CGAGATCAAG
CAGAGGCTGA
AGCTGAAGGA
CGGCGGCCAC
TACGACGCTG
AGGTCAAGAC
CACCTACAAG
GCCAAGAAGC
CCGTGCAGCT
GCCCGGCGCC
TACAACGTCA
ACATCAAGTT
GGACATCACC
TCCCACAACG
AGGACTACAC
CATCGTGGAA
CAGTACGAAC
GCGCCGAGGG
CCGCCACTCC
ACCGGCGGCA
TGGACGAGCT
GTACAAGCGC
CCGCCCCACG
ACCCGCAGCG
CCCGACCGAA
AGGAGCGCAC
GACCCCATGC
ATCGAACAAA
AGAAAAGGGG
ACTGGAAGGG
CTAATTCACT
CCCAACGAAG
ACAAGATATC
ATAACTTCGT
ATAGCATACA
TTATACGAAG
TTATCGGCTA
GCTGGTCCGG
ACTGTACTGG
GTCTCTCTGG
TTAGACCAGA
TCTGAGCCTG
GGAGCTCTCT
GGCTAACTAG
GGAACCCACT
GCTTAAGCCT
CAATAAAGCT
TGCCTTGAGT
GCTTCAAGTA
GTGTGTGCCC
GTCTGTTGTG
TGACTCTGGT
AACTAGAGAT
CCCTCAGACC
CTTTTAGTCA
GTGTGGAAAA
TCTCTAGCAG
GGCCCGTTTA
AACCCGCTGA
TCAGCCTCGA
CTGTGCCTTC
TAGTTGCCAG
CCATCTGTTG
TTTGCCCCTC
CCCCGTGCCT
TCCTTGACCC
TGGAAGGTGC
CACTCCCACT
GTCCTTTCCT
AATAAAATGA
GGAAATTGCA
TCGCATTGTC
TGAGTAGGTG
TCATTCTATT
CTGGGGGGTG
GGGTGGGGCA
GGACAGCAAG
GGGGAGGATT
GGGAAGACAA
TAGCAGGCAT
GCTGGGGATG
CGGTGGGCTC
TATGGCTTCT
GAGGCGGAAA
GAACCAGCTG
GGGCTCTAGG
GGGTATCCCC
ACGCGCCCTG
TAGCGGCGCA
TTAAGCGCGG
CGGGTGTGGT
GGTTACGCGC
AGCGTGACCG
CTACACTTGC
CAGCGCCCTA
GCGCCCGCTC
CTTTCGCTTT
CTTCCCTTCC
TTTCTCGCCA
CGTTCGCCGG
CTTTCCCCGT
CAAGCTCTAA
ATCGGGGGCT
CCCTTTAGGG
TTCCGATTTA
GTGCTTTACG
GCACCTCGAC
CCCAAAAAAC
TTGATTAGGG
TGATGGTTCA
CGTAGTGGGC
CATCGCCCTG
ATAGACGGTT
TTTCGCCCTT
TGACGTTGGA
GTCCACGTTC
TTTAATAGTG
GACTCTTGTT
CCAAACTGGA
ACAACACTCA
ACCCTATCTC
GGTCTATTCT
TTTGATTTAT
AAGGGATTTT
GCCGATTTCG
GCCTATTGGT
TAAAAAATGA
GCTGATTTAA
CAAAAATTTA
ACGCGAATTA
ATTCTGTGGA
ATGTGTGTCA
GTTAGGGTGT
GGAAAGTCCC
CAGGCTCCCC
AGCAGGCAGA
AGTATGCAAA
GCATGCATCT
CAATTAGTCA
GCAACCAGGT
GTGGAAAGTC
CCCAGGCTCC
CCAGCAGGCA
GAAGTATGCA
AAGCATGCAT
CTCAATTAGT
CAGCAACCAT
AGTCCCGCCC
CTAACTCCGC
CCATCCCGCC
CCTAACTCCG
CCCAGTTCCG
CCCATTCTCC
GCCCCATGGC
TGACTAATTT
TTTTTATTTA
TGCAGAGGCC
GAGGCCGCCT
CTGCCTCTGA
GCTATTCCAG
AAGTAGTGAG
GAGGCTTTTT
TGGAGGCCTA
GGCTTTTGCA
AAAAGCTCCC
GGGAGCTTGT
ATATCCATTT
TCGGATCTGA
TCAGCACGTG
TTGACAATTA
ATCATCGGCA
TAGTATATCG
GCATAGTATA
ATACGACAAG
GTGAGGAACT
AAACCATGGC
CAAGTTGACC
AGTGCCGTTC
CGGTGCTCAC
CGCGCGCGAC
GTCGCCGGAG
CGGTCGAGTT
CTGGACCGAC
CGGCTCGGGT
TCTCCCGGGA
CTTCGTGGAG
GACGACTTCG
CCGGTGTGGT
CCGGGACGAC
GTGACCCTGT
TCATCAGCGC
GGTCCAGGAC
CAGGTGGTGC
CGGACAACAC
CCTGGCCTGG
GTGTGGGTGC
GCGGCCTGGA
CGAGCTGTAC
GCCGAGTGGT
CGGAGGTCGT
GTCCACGAAC
TTCCGGGACG
CCTCCGGGCC
GGCCATGACC
GAGATCGGCG
AGCAGCCGTG
GGGGCGGGAG
TTCGCCCTGC
GCGACCCGGC
CGGCAACTGC
GTGCACTTCG
TGGCCGAGGA
GCAGGACTGA
CACGTGCTAC
GAGATTTCGA
TTCCACCGCC
GCCTTCTATG
AAAGGTTGGG
CTTCGGAATC
GTTTTCCGGG
ACGCCGGCTG
GATGATCCTC
CAGCGCGGGG
ATCTCATGCT
GGAGTTCTTC
GCCCACCCCA
ACTTGTTTAT
TGCAGCTTAT
AATGGTTACA
AATAAAGCAA
TAGCATCACA
AATTTCACAA
ATAAAGCATT
TTTTTCACTG
CATTCTAGTT
GTGGTTTGTC
CAAACTCATC
AATGTATCTT
ATCATGTCTG
TATACCGTCG
ACCTCTAGCT
AGAGCTTGGC
GTAATCATGG
TCATAGCTGT
TTCCTGTGTG
AAATTGTTAT
CCGCTCACAA
TTCCACACAA
CATACGAGCC
GGAAGCATAA
AGTGTAAAGC
CTGGGGTGCC
TAATGAGTGA
GCTAACTCAC
ATTAATTGCG
TTGCGCTCAC
TGCCCGCTTT
CCAGTCGGGA
AACCTGTCGT
GCCAGCTGCA
TTAATGAATC
GGCCAACGCG
CGGGGAGAGG
CGGTTTGCGT
ATTGGGCGCT
CTTCCGCTTC
CTCGCTCACT
GACTCGCTGC
GCTCGGTCGT
TCGGCTGCGG
CGAGCGGTAT
CAGCTCACTC
AAAGGCGGTA
ATACGGTTAT
CCACAGAATC
AGGGGATAAC
GCAGGAAAGA
ACATGTGAGC
AAAAGGCCAG
CAAAAGGCCA
GGAACCGTAA
AAAGGCCGCG
TTGCTGGCGT
TTTTCCATAG
GCTCCGCCCC
CCTGACGAGC
ATCACAAAAA
TCGACGCTCA
AGTCAGAGGT
GGCGAAACCC
GACAGGACTA
TAAAGATACC
AGGCGTTTCC
CCCTGGAAGC
TCCCTCGTGC
GCTCTCCTGT
TCCGACCCTG
CCGCTTACCG
GATACCTGTC
CGCCTTTCTC
CCTTCGGGAA
GCGTGGCGCT
TTCTCATAGC
TCACGCTGTA
GGTATCTCAG
TTCGGTGTAG
GTCGTTCGCT
CCAAGCTGGG
CTGTGTGCAC
GAACCCCCCG
TTCAGCCCGA
CCGCTGCGCC
TTATCCGGTA
ACTATCGTCT
TGAGTCCAAC
CCGGTAAGAC
ACGACTTATC
GCCACTGGCA
GCAGCCACTG
GTAACAGGAT
TAGCAGAGCG
AGGTATGTAG
GCGGTGCTAC
AGAGTTCTTG
AAGTGGTGGC
CTAACTACGG
CTACACTAGA
AGAACAGTAT
TTGGTATCTG
CGCTCTGCTG
AAGCCAGTTA
CCTTCGGAAA
AAGAGTTGGT
AGCTCTTGAT
CCGGCAAACA
AACCACCGCT
GGTAGCGGTG
GTTTTTTTGT
TTGCAAGCAG
CAGATTACGC
GCAGAAAAAA
AGGATCTCAA
GAAGATCCTT
TGATCTTTTC
TACGGGGTCT
GACGCTCAGT
GGAACGAAAA
CTCACGTTAA
GGGATTTTGG
TCATGAGATT
ATCAAAAAGG
ATCTTCACCT
AGATCCTTTT
AAATTAAAAA
TGAAGTTTTA
AATCAATCTA
AAGTATATAT
GAGTAAACTT
GGTCTGACAG