名称: | pLV3-U6-SDC3(human)-sgRNA1-Cas9-Puro |
---|---|
别称: | · |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | U6 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | LentiCRISPR V2 |
正向引物: | U6-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTCGACGGAT
CGGGAGATCT
CCCGATCCCC
TATGGTGCAC
TCTCAGTACA
ATCTGCTCTG
ATGCCGCATA
GTTAAGCCAG
TATCTGCTCC
CTGCTTGTGT
GTTGGAGGTC
GCTGAGTAGT
GCGCGAGCAA
AATTTAAGCT
ACAACAAGGC
AAGGCTTGAC
CGACAATTGC
ATGAAGAATC
TGCTTAGGGT
TAGGCGTTTT
GCGCTGCTTC
GCGATGTACG
GGCCAGATAT
ACGCGTTGAC
ATTGATTATT
GACTAGTTAT
TAATAGTAAT
CAATTACGGG
GTCATTAGTT
CATAGCCCAT
ATATGGAGTT
CCGCGTTACA
TAACTTACGG
TAAATGGCCC
GCCTGGCTGA
CCGCCCAACG
ACCCCCGCCC
ATTGACGTCA
ATAATGACGT
ATGTTCCCAT
AGTAACGCCA
ATAGGGACTT
TCCATTGACG
TCAATGGGTG
GAGTATTTAC
GGTAAACTGC
CCACTTGGCA
GTACATCAAG
TGTATCATAT
GCCAAGTACG
CCCCCTATTG
ACGTCAATGA
CGGTAAATGG
CCCGCCTGGC
ATTATGCCCA
GTACATGACC
TTATGGGACT
TTCCTACTTG
GCAGTACATC
TACGTATTAG
TCATCGCTAT
TACCATGGTG
ATGCGGTTTT
GGCAGTACAT
CAATGGGCGT
GGATAGCGGT
TTGACTCACG
GGGATTTCCA
AGTCTCCACC
CCATTGACGT
CAATGGGAGT
TTGTTTTGGC
ACCAAAATCA
ACGGGACTTT
CCAAAATGTC
GTAACAACTC
CGCCCCATTG
ACGCAAATGG
GCGGTAGGCG
TGTACGGTGG
GAGGTCTATA
TAAGCAGCGC
GTTTTGCCTG
TACTGGGTCT
CTCTGGTTAG
ACCAGATCTG
AGCCTGGGAG
CTCTCTGGCT
AACTAGGGAA
CCCACTGCTT
AAGCCTCAAT
AAAGCTTGCC
TTGAGTGCTT
CAAGTAGTGT
GTGCCCGTCT
GTTGTGTGAC
TCTGGTAACT
AGAGATCCCT
CAGACCCTTT
TAGTCAGTGT
GGAAAATCTC
TAGCAGTGGC
GCCCGAACAG
GGACTTGAAA
GCGAAAGGGA
AACCAGAGGA
GCTCTCTCGA
CGCAGGACTC
GGCTTGCTGA
AGCGCGCACG
GCAAGAGGCG
AGGGGCGGCG
ACTGGTGAGT
ACGCCAAAAA
TTTTGACTAG
CGGAGGCTAG
AAGGAGAGAG
ATGGGTGCGA
GAGCGTCAGT
ATTAAGCGGG
GGAGAATTAG
ATCGCGATGG
GAAAAAATTC
GGTTAAGGCC
AGGGGGAAAG
AAAAAATATA
AATTAAAACA
TATAGTATGG
GCAAGCAGGG
AGCTAGAACG
ATTCGCAGTT
AATCCTGGCC
TGTTAGAAAC
ATCAGAAGGC
TGTAGACAAA
TACTGGGACA
GCTACAACCA
TCCCTTCAGA
CAGGATCAGA
AGAACTTAGA
TCATTATATA
ATACAGTAGC
AACCCTCTAT
TGTGTGCATC
AAAGGATAGA
GATAAAAGAC
ACCAAGGAAG
CTTTAGACAA
GATAGAGGAA
GAGCAAAACA
AAAGTAAGAC
CACCGCACAG
CAAGCGGCCG
CTGATCTTCA
GACCTGGAGG
AGGAGATATG
AGGGACAATT
GGAGAAGTGA
ATTATATAAA
TATAAAGTAG
TAAAAATTGA
ACCATTAGGA
GTAGCACCCA
CCAAGGCAAA
GAGAAGAGTG
GTGCAGAGAG
AAAAAAGAGC
AGTGGGAATA
GGAGCTTTGT
TCCTTGGGTT
CTTGGGAGCA
GCAGGAAGCA
CTATGGGCGC
AGCGTCAATG
ACGCTGACGG
TACAGGCCAG
ACAATTATTG
TCTGGTATAG
TGCAGCAGCA
GAACAATTTG
CTGAGGGCTA
TTGAGGCGCA
ACAGCATCTG
TTGCAACTCA
CAGTCTGGGG
CATCAAGCAG
CTCCAGGCAA
GAATCCTGGC
TGTGGAAAGA
TACCTAAAGG
ATCAACAGCT
CCTGGGGATT
TGGGGTTGCT
CTGGAAAACT
CATTTGCACC
ACTGCTGTGC
CTTGGAATGC
TAGTTGGAGT
AATAAATCTC
TGGAACAGAT
TTGGAATCAC
ACGACCTGGA
TGGAGTGGGA
CAGAGAAATT
AACAATTACA
CAAGCTTAAT
ACACTCCTTA
ATTGAAGAAT
CGCAAAACCA
GCAAGAAAAG
AATGAACAAG
AATTATTGGA
ATTAGATAAA
TGGGCAAGTT
TGTGGAATTG
GTTTAACATA
ACAAATTGGC
TGTGGTATAT
AAAATTATTC
ATAATGATAG
TAGGAGGCTT
GGTAGGTTTA
AGAATAGTTT
TTGCTGTACT
TTCTATAGTG
AATAGAGTTA
GGCAGGGATA
TTCACCATTA
TCGTTTCAGA
CCCACCTCCC
AACCCCGAGG
GGACCCGACA
GGCCCGAAGG
AATAGAAGAA
GAAGGTGGAG
AGAGAGACAG
AGACAGATCC
ATTCGATTAG
TGAACGGATC
GGCACTGCGT
GCGCCAATTC
TGCAGACAAA
TGGCAGTATT
CATCCACAAT
TTTAAAAGAA
AAGGGGGGAT
TGGGGGGTAC
AGTGCAGGGG
AAAGAATAGT
AGACATAATA
GCAACAGACA
TACAAACTAA
AGAATTACAA
AAACAAATTA
CAAAAATTCA
AAATTTTCGG
GTTTATTACA
GGGACAGCAG
AGATCCAGTT
TGGTTAATTA
AGGTACCGAG
GGCCTATTTC
CCATGATTCC
TTCATATTTG
CATATACGAT
ACAAGGCTGT
TAGAGAGATA
ATTAGAATTA
ATTTGACTGT
AAACACAAAG
ATATTAGTAC
AAAATACGTG
ACGTAGAAAG
TAATAATTTC
TTGGGTAGTT
TGCAGTTTTA
AAATTATGTT
TTAAAATGGA
CTATCATATG
CTTACCGTAA
CTTGAAAGTA
TTTCGATTTC
TTGGCTTTAT
ATATCTTGTG
GAAAGGACGA
AACACCGGGG
GTGGCGGTGG
CCACTGGTTT
TAGAGCTAGA
AATAGCAAGT
TAAAATAAGG
CTAGTCCGTT
ATCAACTTGA
AAAAGTGGCA
CCGAGTCGGT
GCTTTTTTGA
ATTCGCTAGC
TAGGTCTTGA
AAGGAGTGGG
AATTGGCTCC
GGTGCCCGTC
AGTGGGCAGA
GCGCACATCG
CCCACAGTCC
CCGAGAAGTT
GGGGGGAGGG
GTCGGCAATT
GATCCGGTGC
CTAGAGAAGG
TGGCGCGGGG
TAAACTGGGA
AAGTGATGTC
GTGTACTGGC
TCCGCCTTTT
TCCCGAGGGT
GGGGGAGAAC
CGTATATAAG
TGCAGTAGTC
GCCGTGAACG
TTCTTTTTCG
CAACGGGTTT
GCCGCCAGAA
CACAGGACCG
GTTCTAGAGC
GCTGCCACCA
TGGACAAGAA
GTACAGCATC
GGCCTGGACA
TCGGCACCAA
CTCTGTGGGC
TGGGCCGTGA
TCACCGACGA
GTACAAGGTG
CCCAGCAAGA
AATTCAAGGT
GCTGGGCAAC
ACCGACCGGC
ACAGCATCAA
GAAGAACCTG
ATCGGAGCCC
TGCTGTTCGA
CAGCGGCGAA
ACAGCCGAGG
CCACCCGGCT
GAAGAGAACC
GCCAGAAGAA
GATACACCAG
ACGGAAGAAC
CGGATCTGCT
ATCTGCAAGA
GATCTTCAGC
AACGAGATGG
CCAAGGTGGA
CGACAGCTTC
TTCCACAGAC
TGGAAGAGTC
CTTCCTGGTG
GAAGAGGATA
AGAAGCACGA
GCGGCACCCC
ATCTTCGGCA
ACATCGTGGA
CGAGGTGGCC
TACCACGAGA
AGTACCCCAC
CATCTACCAC
CTGAGAAAGA
AACTGGTGGA
CAGCACCGAC
AAGGCCGACC
TGCGGCTGAT
CTATCTGGCC
CTGGCCCACA
TGATCAAGTT
CCGGGGCCAC
TTCCTGATCG
AGGGCGACCT
GAACCCCGAC
AACAGCGACG
TGGACAAGCT
GTTCATCCAG
CTGGTGCAGA
CCTACAACCA
GCTGTTCGAG
GAAAACCCCA
TCAACGCCAG
CGGCGTGGAC
GCCAAGGCCA
TCCTGTCTGC
CAGACTGAGC
AAGAGCAGAC
GGCTGGAAAA
TCTGATCGCC
CAGCTGCCCG
GCGAGAAGAA
GAATGGCCTG
TTCGGAAACC
TGATTGCCCT
GAGCCTGGGC
CTGACCCCCA
ACTTCAAGAG
CAACTTCGAC
CTGGCCGAGG
ATGCCAAACT
GCAGCTGAGC
AAGGACACCT
ACGACGACGA
CCTGGACAAC
CTGCTGGCCC
AGATCGGCGA
CCAGTACGCC
GACCTGTTTC
TGGCCGCCAA
GAACCTGTCC
GACGCCATCC
TGCTGAGCGA
CATCCTGAGA
GTGAACACCG
AGATCACCAA
GGCCCCCCTG
AGCGCCTCTA
TGATCAAGAG
ATACGACGAG
CACCACCAGG
ACCTGACCCT
GCTGAAAGCT
CTCGTGCGGC
AGCAGCTGCC
TGAGAAGTAC
AAAGAGATTT
TCTTCGACCA
GAGCAAGAAC
GGCTACGCCG
GCTACATTGA
CGGCGGAGCC
AGCCAGGAAG
AGTTCTACAA
GTTCATCAAG
CCCATCCTGG
AAAAGATGGA
CGGCACCGAG
GAACTGCTCG
TGAAGCTGAA
CAGAGAGGAC
CTGCTGCGGA
AGCAGCGGAC
CTTCGACAAC
GGCAGCATCC
CCCACCAGAT
CCACCTGGGA
GAGCTGCACG
CCATTCTGCG
GCGGCAGGAA
GATTTTTACC
CATTCCTGAA
GGACAACCGG
GAAAAGATCG
AGAAGATCCT
GACCTTCCGC
ATCCCCTACT
ACGTGGGCCC
TCTGGCCAGG
GGAAACAGCA
GATTCGCCTG
GATGACCAGA
AAGAGCGAGG
AAACCATCAC
CCCCTGGAAC
TTCGAGGAAG
TGGTGGACAA
GGGCGCTTCC
GCCCAGAGCT
TCATCGAGCG
GATGACCAAC
TTCGATAAGA
ACCTGCCCAA
CGAGAAGGTG
CTGCCCAAGC
ACAGCCTGCT
GTACGAGTAC
TTCACCGTGT
ATAACGAGCT
GACCAAAGTG
AAATACGTGA
CCGAGGGAAT
GAGAAAGCCC
GCCTTCCTGA
GCGGCGAGCA
GAAAAAGGCC
ATCGTGGACC
TGCTGTTCAA
GACCAACCGG
AAAGTGACCG
TGAAGCAGCT
GAAAGAGGAC
TACTTCAAGA
AAATCGAGTG
CTTCGACTCC
GTGGAAATCT
CCGGCGTGGA
AGATCGGTTC
AACGCCTCCC
TGGGCACATA
CCACGATCTG
CTGAAAATTA
TCAAGGACAA
GGACTTCCTG
GACAATGAGG
AAAACGAGGA
CATTCTGGAA
GATATCGTGC
TGACCCTGAC
ACTGTTTGAG
GACAGAGAGA
TGATCGAGGA
ACGGCTGAAA
ACCTATGCCC
ACCTGTTCGA
CGACAAAGTG
ATGAAGCAGC
TGAAGCGGCG
GAGATACACC
GGCTGGGGCA
GGCTGAGCCG
GAAGCTGATC
AACGGCATCC
GGGACAAGCA
GTCCGGCAAG
ACAATCCTGG
ATTTCCTGAA
GTCCGACGGC
TTCGCCAACA
GAAACTTCAT
GCAGCTGATC
CACGACGACA
GCCTGACCTT
TAAAGAGGAC
ATCCAGAAAG
CCCAGGTGTC
CGGCCAGGGC
GATAGCCTGC
ACGAGCACAT
TGCCAATCTG
GCCGGCAGCC
CCGCCATTAA
GAAGGGCATC
CTGCAGACAG
TGAAGGTGGT
GGACGAGCTC
GTGAAAGTGA
TGGGCCGGCA
CAAGCCCGAG
AACATCGTGA
TCGAAATGGC
CAGAGAGAAC
CAGACCACCC
AGAAGGGACA
GAAGAACAGC
CGCGAGAGAA
TGAAGCGGAT
CGAAGAGGGC
ATCAAAGAGC
TGGGCAGCCA
GATCCTGAAA
GAACACCCCG
TGGAAAACAC
CCAGCTGCAG
AACGAGAAGC
TGTACCTGTA
CTACCTGCAG
AATGGGCGGG
ATATGTACGT
GGACCAGGAA
CTGGACATCA
ACCGGCTGTC
CGACTACGAT
GTGGACCATA
TCGTGCCTCA
GAGCTTTCTG
AAGGACGACT
CCATCGACAA
CAAGGTGCTG
ACCAGAAGCG
ACAAGAACCG
GGGCAAGAGC
GACAACGTGC
CCTCCGAAGA
GGTCGTGAAG
AAGATGAAGA
ACTACTGGCG
GCAGCTGCTG
AACGCCAAGC
TGATTACCCA
GAGAAAGTTC
GACAATCTGA
CCAAGGCCGA
GAGAGGCGGC
CTGAGCGAAC
TGGATAAGGC
CGGCTTCATC
AAGAGACAGC
TGGTGGAAAC
CCGGCAGATC
ACAAAGCACG
TGGCACAGAT
CCTGGACTCC
CGGATGAACA
CTAAGTACGA
CGAGAATGAC
AAGCTGATCC
GGGAAGTGAA
AGTGATCACC
CTGAAGTCCA
AGCTGGTGTC
CGATTTCCGG
AAGGATTTCC
AGTTTTACAA
AGTGCGCGAG
ATCAACAACT
ACCACCACGC
CCACGACGCC
TACCTGAACG
CCGTCGTGGG
AACCGCCCTG
ATCAAAAAGT
ACCCTAAGCT
GGAAAGCGAG
TTCGTGTACG
GCGACTACAA
GGTGTACGAC
GTGCGGAAGA
TGATCGCCAA
GAGCGAGCAG
GAAATCGGCA
AGGCTACCGC
CAAGTACTTC
TTCTACAGCA
ACATCATGAA
CTTTTTCAAG
ACCGAGATTA
CCCTGGCCAA
CGGCGAGATC
CGGAAGCGGC
CTCTGATCGA
GACAAACGGC
GAAACCGGGG
AGATCGTGTG
GGATAAGGGC
CGGGATTTTG
CCACCGTGCG
GAAAGTGCTG
AGCATGCCCC
AAGTGAATAT
CGTGAAAAAG
ACCGAGGTGC
AGACAGGCGG
CTTCAGCAAA
GAGTCTATCC
TGCCCAAGAG
GAACAGCGAT
AAGCTGATCG
CCAGAAAGAA
GGACTGGGAC
CCTAAGAAGT
ACGGCGGCTT
CGACAGCCCC
ACCGTGGCCT
ATTCTGTGCT
GGTGGTGGCC
AAAGTGGAAA
AGGGCAAGTC
CAAGAAACTG
AAGAGTGTGA
AAGAGCTGCT
GGGGATCACC
ATCATGGAAA
GAAGCAGCTT
CGAGAAGAAT
CCCATCGACT
TTCTGGAAGC
CAAGGGCTAC
AAAGAAGTGA
AAAAGGACCT
GATCATCAAG
CTGCCTAAGT
ACTCCCTGTT
CGAGCTGGAA
AACGGCCGGA
AGAGAATGCT
GGCCTCTGCC
GGCGAACTGC
AGAAGGGAAA
CGAACTGGCC
CTGCCCTCCA
AATATGTGAA
CTTCCTGTAC
CTGGCCAGCC
ACTATGAGAA
GCTGAAGGGC
TCCCCCGAGG
ATAATGAGCA
GAAACAGCTG
TTTGTGGAAC
AGCACAAGCA
CTACCTGGAC
GAGATCATCG
AGCAGATCAG
CGAGTTCTCC
AAGAGAGTGA
TCCTGGCCGA
CGCTAATCTG
GACAAAGTGC
TGTCCGCCTA
CAACAAGCAC
CGGGATAAGC
CCATCAGAGA
GCAGGCCGAG
AATATCATCC
ACCTGTTTAC
CCTGACCAAT
CTGGGAGCCC
CTGCCGCCTT
CAAGTACTTT
GACACCACCA
TCGACCGGAA
GAGGTACACC
AGCACCAAAG
AGGTGCTGGA
CGCCACCCTG
ATCCACCAGA
GCATCACCGG
CCTGTACGAG
ACACGGATCG
ACCTGTCTCA
GCTGGGAGGC
GACAAGCGAC
CTGCCGCCAC
AAAGAAGGCT
GGACAGGCTA
AGAAGAAGAA
AGATTACAAA
GACGATGACG
ATAAGGGATC
CGGCGCAACA
AACTTCTCTC
TGCTGAAACA
AGCCGGAGAT
GTCGAAGAGA
ATCCTGGACC
GACCGAGTAC
AAGCCCACGG
TGCGCCTCGC
CACCCGCGAC
GACGTCCCCA
GGGCCGTACG
CACCCTCGCC
GCCGCGTTCG
CCGACTACCC
CGCCACGCGC
CACACCGTCG
ATCCGGACCG
CCACATCGAG
CGGGTCACCG
AGCTGCAAGA
ACTCTTCCTC
ACGCGCGTCG
GGCTCGACAT
CGGCAAGGTG
TGGGTCGCGG
ACGACGGCGC
CGCGGTGGCG
GTCTGGACCA
CGCCGGAGAG
CGTCGAAGCG
GGGGCGGTGT
TCGCCGAGAT
CGGCCCGCGC
ATGGCCGAGT
TGAGCGGTTC
CCGGCTGGCC
GCGCAGCAAC
AGATGGAAGG
CCTCCTGGCG
CCGCACCGGC
CCAAGGAGCC
CGCGTGGTTC
CTGGCCACCG
TCGGAGTCTC
GCCCGACCAC
CAGGGCAAGG
GTCTGGGCAG
CGCCGTCGTG
CTCCCCGGAG
TGGAGGCGGC
CGAGCGCGCC
GGGGTGCCCG
CCTTCCTGGA
GACCTCCGCG
CCCCGCAACC
TCCCCTTCTA
CGAGCGGCTC
GGCTTCACCG
TCACCGCCGA
CGTCGAGGTG
CCCGAAGGAC
CGCGCACCTG
GTGCATGACC
CGCAAGCCCG
GTGCCTGAAC
GCGTTAAGTC
GACAATCAAC
CTCTGGATTA
CAAAATTTGT
GAAAGATTGA
CTGGTATTCT
TAACTATGTT
GCTCCTTTTA
CGCTATGTGG
ATACGCTGCT
TTAATGCCTT
TGTATCATGC
TATTGCTTCC
CGTATGGCTT
TCATTTTCTC
CTCCTTGTAT
AAATCCTGGT
TGCTGTCTCT
TTATGAGGAG
TTGTGGCCCG
TTGTCAGGCA
ACGTGGCGTG
GTGTGCACTG
TGTTTGCTGA
CGCAACCCCC
ACTGGTTGGG
GCATTGCCAC
CACCTGTCAG
CTCCTTTCCG
GGACTTTCGC
TTTCCCCCTC
CCTATTGCCA
CGGCGGAACT
CATCGCCGCC
TGCCTTGCCC
GCTGCTGGAC
AGGGGCTCGG
CTGTTGGGCA
CTGACAATTC
CGTGGTGTTG
TCGGGGAAAT
CATCGTCCTT
TCCTTGGCTG
CTCGCCTGTG
TTGCCACCTG
GATTCTGCGC
GGGACGTCCT
TCTGCTACGT
CCCTTCGGCC
CTCAATCCAG
CGGACCTTCC
TTCCCGCGGC
CTGCTGCCGG
CTCTGCGGCC
TCTTCCGCGT
CTTCGCCTTC
GCCCTCAGAC
GAGTCGGATC
TCCCTTTGGG
CCGCCTCCCC
GCGTCGACTT
TAAGACCAAT
GACTTACAAG
GCAGCTGTAG
ATCTTAGCCA
CTTTTTAAAA
GAAAAGGGGG
GACTGGAAGG
GCTAATTCAC
TCCCAACGAA
GACAAGATCT
GCTTTTTGCT
TGTACTGGGT
CTCTCTGGTT
AGACCAGATC
TGAGCCTGGG
AGCTCTCTGG
CTAACTAGGG
AACCCACTGC
TTAAGCCTCA
ATAAAGCTTG
CCTTGAGTGC
TTCAAGTAGT
GTGTGCCCGT
CTGTTGTGTG
ACTCTGGTAA
CTAGAGATCC
CTCAGACCCT
TTTAGTCAGT
GTGGAAAATC
TCTAGCAGGG
CCCGTTTAAA
CCCGCTGATC
AGCCTCGACT
GTGCCTTCTA
GTTGCCAGCC
ATCTGTTGTT
TGCCCCTCCC
CCGTGCCTTC
CTTGACCCTG
GAAGGTGCCA
CTCCCACTGT
CCTTTCCTAA
TAAAATGAGG
AAATTGCATC
GCATTGTCTG
AGTAGGTGTC
ATTCTATTCT
GGGGGGTGGG
GTGGGGCAGG
ACAGCAAGGG
GGAGGATTGG
GAAGACAATA
GCAGGCATGC
TGGGGATGCG
GTGGGCTCTA
TGGCTTCTGA
GGCGGAAAGA
ACCAGCTGGG
GCTCTAGGGG
GTATCCCCAC
GCGCCCTGTA
GCGGCGCATT
AAGCGCGGCG
GGTGTGGTGG
TTACGCGCAG
CGTGACCGCT
ACACTTGCCA
GCGCCCTAGC
GCCCGCTCCT
TTCGCTTTCT
TCCCTTCCTT
TCTCGCCACG
TTCGCCGGCT
TTCCCCGTCA
AGCTCTAAAT
CGGGGGCTCC
CTTTAGGGTT
CCGATTTAGT
GCTTTACGGC
ACCTCGACCC
CAAAAAACTT
GATTAGGGTG
ATGGTTCACG
TAGTGGGCCA
TCGCCCTGAT
AGACGGTTTT
TCGCCCTTTG
ACGTTGGAGT
CCACGTTCTT
TAATAGTGGA
CTCTTGTTCC
AAACTGGAAC
AACACTCAAC
CCTATCTCGG
TCTATTCTTT
TGATTTATAA
GGGATTTTGC
CGATTTCGGC
CTATTGGTTA
AAAAATGAGC
TGATTTAACA
AAAATTTAAC
GCGAATTAAT
TCTGTGGAAT
GTGTGTCAGT
TAGGGTGTGG
AAAGTCCCCA
GGCTCCCCAG
CAGGCAGAAG
TATGCAAAGC
ATGCATCTCA
ATTAGTCAGC
AACCAGGTGT
GGAAAGTCCC
CAGGCTCCCC
AGCAGGCAGA
AGTATGCAAA
GCATGCATCT
CAATTAGTCA
GCAACCATAG
TCCCGCCCCT
AACTCCGCCC
ATCCCGCCCC
TAACTCCGCC
CAGTTCCGCC
CATTCTCCGC
CCCATGGCTG
ACTAATTTTT
TTTATTTATG
CAGAGGCCGA
GGCCGCCTCT
GCCTCTGAGC
TATTCCAGAA
GTAGTGAGGA
GGCTTTTTTG
GAGGCCTAGG
CTTTTGCAAA
AAGCTCCCGG
GAGCTTGTAT
ATCCATTTTC
GGATCTGATC
AGCACGTGTT
GACAATTAAT
CATCGGCATA
GTATATCGGC
ATAGTATAAT
ACGACAAGGT
GAGGAACTAA
ACCATGGCCA
AGTTGACCAG
TGCCGTTCCG
GTGCTCACCG
CGCGCGACGT
CGCCGGAGCG
GTCGAGTTCT
GGACCGACCG
GCTCGGGTTC
TCCCGGGACT
TCGTGGAGGA
CGACTTCGCC
GGTGTGGTCC
GGGACGACGT
GACCCTGTTC
ATCAGCGCGG
TCCAGGACCA
GGTGGTGCCG
GACAACACCC
TGGCCTGGGT
GTGGGTGCGC
GGCCTGGACG
AGCTGTACGC
CGAGTGGTCG
GAGGTCGTGT
CCACGAACTT
CCGGGACGCC
TCCGGGCCGG
CCATGACCGA
GATCGGCGAG
CAGCCGTGGG
GGCGGGAGTT
CGCCCTGCGC
GACCCGGCCG
GCAACTGCGT
GCACTTCGTG
GCCGAGGAGC
AGGACTGACA
CGTGCTACGA
GATTTCGATT
CCACCGCCGC
CTTCTATGAA
AGGTTGGGCT
TCGGAATCGT
TTTCCGGGAC
GCCGGCTGGA
TGATCCTCCA
GCGCGGGGAT
CTCATGCTGG
AGTTCTTCGC
CCACCCCAAC
TTGTTTATTG
CAGCTTATAA
TGGTTACAAA
TAAAGCAATA
GCATCACAAA
TTTCACAAAT
AAAGCATTTT
TTTCACTGCA
TTCTAGTTGT
GGTTTGTCCA
AACTCATCAA
TGTATCTTAT
CATGTCTGTA
TACCGTCGAC
CTCTAGCTAG
AGCTTGGCGT
AATCATGGTC
ATAGCTGTTT
CCTGTGTGAA
ATTGTTATCC
GCTCACAATT
CCACACAACA
TACGAGCCGG
AAGCATAAAG
TGTAAAGCCT
GGGGTGCCTA
ATGAGTGAGC
TAACTCACAT
TAATTGCGTT
GCGCTCACTG
CCCGCTTTCC
AGTCGGGAAA
CCTGTCGTGC
CAGCTGCATT
AATGAATCGG
CCAACGCGCG
GGGAGAGGCG
GTTTGCGTAT
TGGGCGCTCT
TCCGCTTCCT
CGCTCACTGA
CTCGCTGCGC
TCGGTCGTTC
GGCTGCGGCG
AGCGGTATCA
GCTCACTCAA
AGGCGGTAAT
ACGGTTATCC
ACAGAATCAG
GGGATAACGC
AGGAAAGAAC
ATGTGAGCAA
AAGGCCAGCA
AAAGGCCAGG
AACCGTAAAA
AGGCCGCGTT
GCTGGCGTTT
TTCCATAGGC
TCCGCCCCCC
TGACGAGCAT
CACAAAAATC
GACGCTCAAG
TCAGAGGTGG
CGAAACCCGA
CAGGACTATA
AAGATACCAG
GCGTTTCCCC
CTGGAAGCTC
CCTCGTGCGC
TCTCCTGTTC
CGACCCTGCC
GCTTACCGGA
TACCTGTCCG
CCTTTCTCCC
TTCGGGAAGC
GTGGCGCTTT
CTCATAGCTC
ACGCTGTAGG
TATCTCAGTT
CGGTGTAGGT
CGTTCGCTCC
AAGCTGGGCT
GTGTGCACGA
ACCCCCCGTT
CAGCCCGACC
GCTGCGCCTT
ATCCGGTAAC
TATCGTCTTG
AGTCCAACCC
GGTAAGACAC
GACTTATCGC
CACTGGCAGC
AGCCACTGGT
AACAGGATTA
GCAGAGCGAG
GTATGTAGGC
GGTGCTACAG
AGTTCTTGAA
GTGGTGGCCT
AACTACGGCT
ACACTAGAAG
AACAGTATTT
GGTATCTGCG
CTCTGCTGAA
GCCAGTTACC
TTCGGAAAAA
GAGTTGGTAG
CTCTTGATCC
GGCAAACAAA
CCACCGCTGG
TAGCGGTGGT
TTTTTTGTTT
GCAAGCAGCA
GATTACGCGC
AGAAAAAAAG
GATCTCAAGA
AGATCCTTTG
ATCTTTTCTA
CGGGGTCTGA
CGCTCAGTGG
AACGAAAACT
CACGTTAAGG
GATTTTGGTC
ATGAGATTAT
CAAAAAGGAT
CTTCACCTAG
ATCCTTTTAA
ATTAAAAATG
AAGTTTTAAA
TCAATCTAAA
GTATATATGA
GTAAACTTGG
TCTGACAGTT
ACCAATGCTT
AATCAGTGAG
GCACCTATCT
CAGCGATCTG
TCTATTTCGT
TCATCCATAG
TTGCCTGACT
CCCCGTCGTG
TAGATAACTA
CGATACGGGA
GGGCTTACCA
TCTGGCCCCA
GTGCTGCAAT
GATACCGCGA
GACCCACGCT
CACCGGCTCC
AGATTTATCA
GCAATAAACC
AGCCAGCCGG
AAGGGCCGAG
CGCAGAAGTG
GTCCTGCAAC
TTTATCCGCC
TCCATCCAGT
CTATTAATTG
TTGCCGGGAA
GCTAGAGTAA
GTAGTTCGCC
AGTTAATAGT
TTGCGCAACG
TTGTTGCCAT
TGCTACAGGC
ATCGTGGTGT
CACGCTCGTC
GTTTGGTATG
GCTTCATTCA
GCTCCGGTTC
CCAACGATCA
AGGCGAGTTA
CATGATCCCC
CATGTTGTGC
AAAAAAGCGG
TTAGCTCCTT
CGGTCCTCCG
ATCGTTGTCA
GAAGTAAGTT
GGCCGCAGTG
TTATCACTCA
TGGTTATGGC
AGCACTGCAT
AATTCTCTTA
CTGTCATGCC
ATCCGTAAGA
TGCTTTTCTG
TGACTGGTGA
GTACTCAACC
AAGTCATTCT
GAGAATAGTG
TATGCGGCGA
CCGAGTTGCT
CTTGCCCGGC
GTCAATACGG
GATAATACCG
CGCCACATAG
CAGAACTTTA
AAAGTGCTCA
TCATTGGAAA
ACGTTCTTCG
GGGCGAAAAC
TCTCAAGGAT
CTTACCGCTG
TTGAGATCCA
GTTCGATGTA
ACCCACTCGT
GCACCCAACT
GATCTTCAGC
ATCTTTTACT
TTCACCAGCG
TTTCTGGGTG
AGCAAAAACA
GGAAGGCAAA
ATGCCGCAAA
AAAGGGAATA
AGGGCGACAC
GGAAATGTTG
AATACTCATA
CTCTTCCTTT
TTCAATATTA
TTGAAGCATT
TATCAGGGTT
ATTGTCTCAT
GAGCGGATAC
ATATTTGAAT
GTATTTAGAA
AAATAAACAA
ATAGGGGTTC
CGCGCACATT
TCCCCGAAAA
GTGCCACCTG
AC