名称: | pGBKT7-SPO11(human) |
---|---|
别称: | |
基因长: | |
质粒宿主: | 酵母菌 |
质粒用途: | 杂交 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Kan |
筛选标记: | TRP1 |
荧光标记: | |
启动子: | ADH1 |
复制子: | 2U |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | pGBKT7 |
正向引物: | ADH1-R |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CGGTGCGGGC
CTCTTCGCTA
TTACGCCAGA
TCCTTTTGTT
GTTTCCGGGT
GTACAATATG
GACTTCCTCT
TTTCTGGCAA
CCAAACCCAT
ACATCGGGAT
TCCTATAATA
CCTTCGTTGG
TCTCCCTAAC
ATGTAGGTGG
CGGAGGGGAG
ATATACAATA
GAACAGATAC
CAGACAAGAC
ATAATGGGCT
AAACAAGACT
ACACCAATTA
CACTGCCTCA
TTGATGGTGG
TACATAACGA
ACTAATACTG
TAGCCCTAGA
CTTGATAGCC
ATCATCATAT
CGAAGTTTCA
CTACCCTTTT
TCCATTTGCC
ATCTATTGAA
GTAATAATAG
GCGCATGCAA
CTTCTTTTCT
TTTTTTTTCT
TTTCTCTCTC
CCCCGTTGTT
GTCTCACCAT
ATCCGCAATG
ACAAAAAAAT
GATGGAAGAC
ACTAAAGGAA
AAAATTAACG
ACAAAGACAG
CACCAACAGA
TGTCGTTGTT
CCAGAGCTGA
TGAGGGGTAT
CTCGAAGCAC
ACGAAACTTT
TTCCTTCCTT
CATTCACGCA
CACTACTCTC
TAATGAGCAA
CGGTATACGG
CCTTCCTTCC
AGTTACTTGA
ATTTGAAATA
AAAAAAAGTT
TGCTGTCTTG
CTATCAAGTA
TAAATAGACC
TGCAATTATT
AATCTTTTGT
TTCCTCGTCA
TTGTTCTCGT
TCCCTTTCTT
CCTTGTTTCT
TTTTCTGCAC
AATATTTCAA
GCTATACCAA
GCATACAATC
AACTCCAAGC
TTGAAGCAAG
CCTCCTGAAA
GATGAAGCTA
CTGTCTTCTA
TCGAACAAGC
ATGCGATATT
TGCCGACTTA
AAAAGCTCAA
GTGCTCCAAA
GAAAAACCGA
AGTGCGCCAA
GTGTCTGAAG
AACAACTGGG
AGTGTCGCTA
CTCTCCCAAA
ACCAAAAGGT
CTCCGCTGAC
TAGGGCACAT
CTGACAGAAG
TGGAATCAAG
GCTAGAAAGA
CTGGAACAGC
TATTTCTACT
GATTTTTCCT
CGAGAAGACC
TTGACATGAT
TTTGAAAATG
GATTCTTTAC
AGGATATAAA
AGCATTGTTA
ACAGGATTAT
TTGTACAAGA
TAATGTGAAT
AAAGATGCCG
TCACAGATAG
ATTGGCTTCA
GTGGAGACTG
ATATGCCTCT
AACATTGAGA
CAGCATAGAA
TAAGTGCGAC
ATCATCATCG
GAAGAGAGTA
GTAACAAAGG
TCAAAGACAG
TTGACTGTAT
CGCCGGAATT
TGTAATACGA
CTCACTATAG
GGCGAGCCGC
CATCATGGAG
GAGCAGAAGC
TGATCTCAGA
GGAGGACCTG
CATATGGCCT
TTGCACCTAT
GGGGCCCGAG
GCCTCGTTCT
TCGACGTTTT
GGACCGACAC
AGGGAGTCCC
TGCTGGCTGC
CCTGAGGAGA
GGTGGCAGGG
AGCCCCCAAC
TGGGGGAAGC
CGCCTGGCCT
CCAGTTCTGA
GGTTCTTGCA
TCTATAGAAA
ATATTATCCA
AGACATAATC
ACAAGCTTGG
CAAGAAATGA
AGCACCTGCA
TTCACGATAG
ACAACAGATC
AAGCTGGGAA
AACATAAAGT
TTGAAGATTC
TGTGGGTCTT
CAGATGGTAT
CCCATTGCAC
CACCAGAAAG
ATCAAAAGTG
ATTCACCAAA
ATCAGCTCAA
AAATTTTCTC
TAATCCTTAA
AATATTGTCC
ATGATTTATA
AATTAGTACA
GAGCAACACT
TATGCAACCA
AAAGGGACAT
ATATTACACT
GACAGTCAAC
TCTTTGGTAA
CCAGACTGTC
GTCGACAATA
TTATCAATGA
CATTTCTTGC
ATGTTAAAAG
TGTCAAGGAG
GAGTCTACAT
ATATTATCTA
CATCAAAAGG
TTTAATTGCT
GGCAACTTAA
GATACATCGA
GGAAGATGGC
ACCAAAGTGA
ATTGTACCTG
TGGTGCAACG
GCTGTTGCTG
TGCCATCGAA
TATTCAAGGA
ATTCGGAATT
TAGTTACAGA
TGCAAAGTTT
GTATTAATTG
TAGAAAAAGA
TGCAACATTT
CAGCGGCTCC
TAGATGACAA
CTTTTGCAAC
AAATTGTCTC
CTTGCATCAT
GATTACGGGA
AAGGGAGTTC
CTGATCTAAA
CACAAGACTT
TTAGTCAAGA
AACTGTGGGA
TACATTTCAT
GTTCCTGTTT
TCACTCTTGT
AGATGCTGAT
CCACATGGCA
TAGAAATAAT
GTGCATCTAT
AAGTATGGAT
CTATGTCTAT
GTCTTTTGAA
GCTCATCATC
TCACAGTTCC
AGCTATTAGA
TGGCTTGGTC
TTCTCCCTTC
TGATCTTAAA
AGATTAAATG
TACCTAAAGA
TAGTTTGATT
CCACTGACAA
AAAGGGACCA
AATGAAACTT
GACAGTATCC
TGAGGAGACC
TTATGTTACC
TGCCAACCAT
TTTGGAGAAA
AGAAATGGAA
ATAATGGCAG
ACTCTAAAAT
GAAGGCAGAA
ATTCAAGCTT
TGACTTTCCT
ATCATCAGAT
TATCTTTCCA
GAGTGTACTT
ACCTAACAAA
TTAAAATTTG
GAGGATGGAT
ATAAGCGGCC
GCATAACTAG
CATAACCCCT
TGGGGCCTCT
AAACGGGTCT
TGAGGGGTTT
TTTGCGCGCT
TGCAGCCAAG
CTAATTCCGG
GCGAATTTCT
TATGATTTAT
GATTTTTATT
ATTAAATAAG
TTATAAAAAA
AATAAGTGTA
TACAAATTTT
AAAGTGACTC
TTAGGTTTTA
AAACGAAAAT
TCTTATTCTT
GAGTAACTCT
TTCCTGTAGG
TCAGGTTGCT
TTCTCAGGTA
TAGCATGAGG
TCGCTCTTAT
TGACCACACC
TCTACCGGCA
TGCAAGCTTG
GCGTAATCAT
GGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGAACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAACC
TGAGGCTATG
GCAGGGCCTG
CCGCCCCGAC
GTTGGCTGCG
AGCCCTGGGC
CTTCACCCGA
ACTTGGGGGG
TGGGGTGGGG
AAAAGGAAGA
AACGCGGGCG
TATTGGCCCC
AATGGGGTCT
CGGTGGGGTA
TCGACAGAGT
GCCAGCCCTG
GGACCGAACC
CCGCGTTTAT
GAACAAACGA
CCCAACACCG
TGCGTTTTAT
TCTGTCTTTT
TATTGCCGTC
ATAGCGCGGG
TTCCTTCCGG
TATTGTCTCC
TTCCGTGTTT
CAGTTAGCCT
CCCCCTAGGG
TGGGCGAAGA
ACTCCAGCAT
GAGATCCCCG
CGCTGGAGGA
TCATCCAGCC
GGCGTCCCGG
AAAACGATTC
CGAAGCCCAA
CCTTTCATAG
AAGGCGGCGG
TGGAATCGAA
ATCTCGTGAT
GGCAGGTTGG
GCGTCGCTTG
GTCGGTCATT
TCGAACCCCA
GAGTCCCGCT
CAGAAGAACT
CGTCAAGAAG
GCGATAGAAG
GCGATGCGCT
GCGAATCGGG
AGCGGCGATA
CCGTAAAGCA
CGAGGAAGCG
GTCAGCCCAT
TCGCCGCCAA
GCTCTTCAGC
AATATCACGG
GTAGCCAACG
CTATGTCCTG
ATAGCGGTCC
GCCACACCCA
GCCGGCCACA
GTCGATGAAT
CCAGAAAAGC
GGCCATTTTC
CACCATGATA
TTCGGCAAGC
AGGCATCGCC
ATGAGTCACG
ACGAGATCCT
CGCCGTCGGG
CATGCTCGCC
TTGAGCCTGG
CGAACAGTTC
GGCTGGCGCG
AGCCCCTGAT
GCTCTTCGTC
CAGATCATCC
TGATCGACAA
GACCGGCTTC
CATCCGAGTA
CGTGCTCGCT
CGATGCGATG
TTTCGCTTGG
TGGTCGAATG
GGCAGGTAGC
CGGATCAAGC
GTATGCAGCC
GCCGCATTGC
ATCAGCCATG
ATGGATACTT
TCTCGGCAGG
AGCAAGGTGA
GATGACAGGA
GATCCTGCCC
CGGCACTTCG
CCCAATAGCA
GCCAGTCCCT
TCCCGCTTCA
GTGACAACGT
CGAGCACAGC
TGCGCAAGGA
ACGCCCGTCG
TGGCCAGCCA
CGATAGCCGC
GCTGCCTCGT
CTTGCAGTTC
ATTCAGGGCA
CCGGACAGGT
CGGTCTTGAC
AAAAAGAACC
GGGCGCCCCT
GCGCTGACAG
CCGGAACACG
GCGGCATCAG
AGCAGCCGAT
TGTCTGTTGT
GCCCAGTCAT
AGCCGAATAG
CCTCTCCACC
CAAGCGGCCG
GAGAACCTGC
GTGCAATCCA
TCTTGTTCAA
TCATACTCTT
CCTTTTTCAA
TATTATTGAA
GCATTTATCA
GGGTTATTGT
CTCATGAGCG
GATACATATT
TGAATGTATT
TAGAAAAATA
AACAAATAGG
GGTTCCGCGC
ACATTTCCCC
GAAAAGTGCC
ACCTGAACGA
AGCATCTGTG
CTTCATTTTG
TAGAACAAAA
ATGCAACGCG
AGAGCGCTAA
TTTTTCAAAC
AAAGAATCTG
AGCTGCATTT
TTACAGAACA
GAAATGCAAC
GCGAAAGCGC
TATTTTACCA
ACGAAGAATC
TGTGCTTCAT
TTTTGTAAAA
CAAAAATGCA
ACGCGAGAGC
GCTAATTTTT
CAAACAAAGA
ATCTGAGCTG
CATTTTTACA
GAACAGAAAT
GCAACGCGAG
AGCGCTATTT
TACCAACAAA
GAATCTATAC
TTCTTTTTTG
TTCTACAAAA
ATGCATCCCG
AGAGCGCTAT
TTTTCTAACA
AAGCATCTTA
GATTACTTTT
TTTCTCCTTT
GTGCGCTCTA
TAATGCAGTC
TCTTGATAAC
TTTTTGCACT
GTAGGTCCGT
TAAGGTTAGA
AGAAGGCTAC
TTTGGTGTCT
ATTTTCTCTT
CCATAAAAAA
AGCCTGACTC
CACTTCCCGC
GTTTACTGAT
TACTAGCGAA
GCTGCGGGTG
CATTTTTTCA
AGATAAAGGC
ATCCCCGATT
ATATTCTATA
CCGATGTGGA
TTGCGCATAC
TTTGTGAACA
GAAAGTGATA
GCGTTGATGA
TTCTTCATTG
GTCAGAAAAT
TATGAACGGT
TTCTTCTATT
TTGTCTCTAT
ATACTACGTA
TAGGAAATGT
TTACATTTTC
GTATTGTTTT
CGATTCACTC
TATGAATAGT
TCTTACTACA
ATTTTTTTGT
CTAAAGAGTA
ATACTAGAGA
TAAACATAAA
AAATGTAGAG
GTCGAGTTTA
GATGCAAGTT
CAAGGAGCGA
AAGGTGGATG
GGTAGGTTAT
ATAGGGATAT
AGCACAGAGA
TATATAGCAA
AGAGATACTT
TTGAGCAATG
TTTGTGGAAG
CGGTATTCGC
AATATTTTAG
TAGCTCGTTA
CAGTCCGGTG
CGTTTTTGGT
TTTTTGAAAG
TGCGTCTTCA
GAGCGCTTTT
GGTTTTCAAA
AGCGCTCTGA
AGTTCCTATA
CTTTCTAGAG
AATAGGAACT
TCGGAATAGG
AACTTCAAAG
CGTTTCCGAA
AACGAGCGCT
TCCGAAAATG
CAACGCGAGC
TGCGCACATA
CAGCTCACTG
TTCACGTCGC
ACCTATATCT
GCGTGTTGCC
TGTATATATA
TATACATGAG
AAGAACGGCA
TAGTGCGTGT
TTATGCTTAA
ATGCGTACTT
ATATGCGTCT
ATTTATGTAG
GATGAAAGGT
AGTCTAGTAC
CTCCTGTGAT
ATTATCCCAT
TCCATGCGGG
GTATCGTATG
CTTCCTTCAG
CACTACCCTT
TAGCTGTTCT
ATATGCTGCC
ACTCCTCAAT
TGGATTAGTC
TCATCCTTCA
ATGCTATCAT
TTCCTTTGAT
ATTGGATCAT
ACTAAGAAAC
CATTATTATC
ATGACATTAA
CCTATAAAAA
TAGGCGTATC
ACGAGGCCCT
TTCGTCTCGC
GCGTTTCGGT
GATGACGGTG
AAAACCTCTG
ACACATGCAG
CTCCCGGAGA
CGGTCACAGC
TTGTCTGTAA
GCGGATGCCG
GGAGCAGACA
AGCCCGTCAG
GGCGCGTCAG
CGGGTGTTGG
CGGGTGTCGG
GGCTGGCTTA
ACTATGCGGC
ATCAGAGCAG
ATTGTACTGA
GAGTGCACCA
TAGATCAACG
ACATTACTAT
ATATATAATA
TAGGAAGCAT
TTAATAGAAC
AGCATCGTAA
TATATGTGTA
CTTTGCAGTT
ATGACGCCAG
ATGGCAGTAG
TGGAAGATAT
TCTTTATTGA
AAAATAGCTT
GTCACCTTAC
GTACAATCTT
GATCCGGAGC
TTTTCTTTTT
TTGCCGATTA
AGAATTAATT
CGGTCGAAAA
AAGAAAAGGA
GAGGGCCAAG
AGGGAGGGCA
TTGGTGACTA
TTGAGCACGT
GAGTATACGT
GATTAAGCAC
ACAAAGGCAG
CTTGGAGTAT
GTCTGTTATT
AATTTCACAG
GTAGTTCTGG
TCCATTGGTG
AAAGTTTGCG
GCTTGCAGAG
CACAGAGGCC
GCAGAATGTG
CTCTAGATTC
CGATGCTGAC
TTGCTGGGTA
TTATATGTGT
GCCCAATAGA
AAGAGAACAA
TTGACCCGGT
TATTGCAAGG
AAAATTTCAA
GTCTTGTAAA
AGCATATAAA
AATAGTTCAG
GCACTCCGAA
ATACTTGGTT
GGCGTGTTTC
GTAATCAACC
TAAGGAGGAT
GTTTTGGCTC
TGGTCAATGA
TTACGGCATT
GATATCGTCC
AACTGCATGG
AGATGAGTCG
TGGCAAGAAT
ACCAAGAGTT
CCTCGGTTTG
CCAGTTATTA
AAAGACTCGT
ATTTCCAAAA
GACTGCAACA
TACTACTCAG
TGCAGCTTCA
CAGAAACCTC
ATTCGTTTAT
TCCCTTGTTT
GATTCAGAAG
CAGGTGGGAC
AGGTGAACTT
TTGGATTGGA
ACTCGATTTC
TGACTGGGTT
GGAAGGCAAG
AGAGCCCCGA
AAGCTTACAT
TTTATGTTAG
CTGGTGGACT
GACGCCAGAA
AATGTTGGTG
ATGCGCTTAG
ATTAAATGGC
GTTATTGGTG
TTGATGTAAG
CGGAGGTGTG
GAGACAAATG
GTGTAAAAGA
CTCTAACAAA
ATAGCAAATT
TCGTCAAAAA
TGCTAAGAAA
TAGGTTATTA
CTGAGTAGTA
TTTATTTAAG
TATTGTTTGT
GCACTTGCCG
ATCTATGCGG
TGTGAAATAC
CGCACAGATG
CGTAAGGAGA
AAATACCGCA
TCAGGAAATT
GTAAGCGTTA
ATATTTTGTT
AAAATTCGCG
TTAAATTTTT
GTTAAATCAG
CTCATTTTTT
AACCAATAGG
CCGAAATCGG
CAAAATCCCT
TATAAATCAA
AAGAATAGAC
CGAGATAGGG
TTGAGTGTTG
TTCCAGTTTG
GAACAAGAGT
CCACTATTAA
AGAACGTGGA
CTCCAACGTC
AAAGGGCGAA
AAACCGTCTA
TCAGGGCGAT
GGCCCACTAC
GTGAACCATC
ACCCTAATCA
AGTTTTTTGG
GGTCGAGGTG
CCGTAAAGCA
CTAAATCGGA
ACCCTAAAGG
GAGCCCCCGA
TTTAGAGCTT
GACGGGGAAA
GCCGGCGAAC
GTGGCGAGAA
AGGAAGGGAA
GAAAGCGAAA
GGAGCGGGCG
CTAGGGCGCT
GGCAAGTGTA
GCGGTCACGC
TGCGCGTAAC
CACCACACCC
GCCGCGCTTA
ATGCGCCGCT
ACAGGGCGCG
TCCATTCGCC
ATTCAGGCTG
CGCAACTGTT
GGGAAGGGCG
AT