产品货号：P60104
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pU6-Brs3(rat)-sgRNA2-Cas9-Puro |
|---|---|
| 别称: | - |
| 基因长: | |
| 质粒宿主： | 哺乳细胞 |
| 质粒用途： | 基因编辑 |
| 片段类型： | CRISPR |
| 片段物种： | 大鼠 |
| 原核抗性： | Amp |
| 筛选标记： | Puro |
| 荧光标记： | |
| 启动子: | U6 |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | pX459 V2.0 |
| 正向引物: | U6-F |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
GAGGGCCTAT
TTCCCATGAT
TCCTTCATAT
TTGCATATAC
GATACAAGGC
TGTTAGAGAG
ATAATTGGAA
TTAATTTGAC
TGTAAACACA
AAGATATTAG
TACAAAATAC
GTGACGTAGA
AAGTAATAAT
TTCTTGGGTA
GTTTGCAGTT
TTAAAATTAT
GTTTTAAAAT
GGACTATCAT
ATGCTTACCG
TAACTTGAAA
GTATTTCGAT
TTCTTGGCTT
TATATATCTT
GTGGAAAGGA
CGAAACACCG
TGATGAGGAT
AGCATTTCCG
GTTTTAGAGC
TAGAAATAGC
AAGTTAAAAT
AAGGCTAGTC
CGTTATCAAC
TTGAAAAAGT
GGCACCGAGT
CGGTGCTTTT
TTGTTTTAGA
GCTAGAAATA
GCAAGTTAAA
ATAAGGCTAG
TCCGTTTTTA
GCGCGTGCGC
CAATTCTGCA
GACAAATGGC
TCTAGAGGTA
CCCGTTACAT
AACTTACGGT
AAATGGCCCG
CCTGGCTGAC
CGCCCAACGA
CCCCCGCCCA
TTGACGTCAA
TAGTAACGCC
AATAGGGACT
TTCCATTGAC
GTCAATGGGT
GGAGTATTTA
CGGTAAACTG
CCCACTTGGC
AGTACATCAA
GTGTATCATA
TGCCAAGTAC
GCCCCCTATT
GACGTCAATG
ACGGTAAATG
GCCCGCCTGG
CATTGTGCCC
AGTACATGAC
CTTATGGGAC
TTTCCTACTT
GGCAGTACAT
CTACGTATTA
GTCATCGCTA
TTACCATGGT
CGAGGTGAGC
CCCACGTTCT
GCTTCACTCT
CCCCATCTCC
CCCCCCTCCC
CACCCCCAAT
TTTGTATTTA
TTTATTTTTT
AATTATTTTG
TGCAGCGATG
GGGGCGGGGG
GGGGGGGGGG
GCGCGCGCCA
GGCGGGGCGG
GGCGGGGCGA
GGGGCGGGGC
GGGGCGAGGC
GGAGAGGTGC
GGCGGCAGCC
AATCAGAGCG
GCGCGCTCCG
AAAGTTTCCT
TTTATGGCGA
GGCGGCGGCG
GCGGCGGCCC
TATAAAAAGC
GAAGCGCGCG
GCGGGCGGGA
GTCGCTGCGA
CGCTGCCTTC
GCCCCGTGCC
CCGCTCCGCC
GCCGCCTCGC
GCCGCCCGCC
CCGGCTCTGA
CTGACCGCGT
TACTCCCACA
GGTGAGCGGG
CGGGACGGCC
CTTCTCCTCC
GGGCTGTAAT
TAGCTGAGCA
AGAGGTAAGG
GTTTAAGGGA
TGGTTGGTTG
GTGGGGTATT
AATGTTTAAT
TACCTGGAGC
ACCTGCCTGA
AATCACTTTT
TTTCAGGTTG
GACCGGTGCC
ACCATGGACT
ATAAGGACCA
CGACGGAGAC
TACAAGGATC
ATGATATTGA
TTACAAAGAC
GATGACGATA
AGATGGCCCC
AAAGAAGAAG
CGGAAGGTCG
GTATCCACGG
AGTCCCAGCA
GCCGACAAGA
AGTACAGCAT
CGGCCTGGAC
ATCGGCACCA
ACTCTGTGGG
CTGGGCCGTG
ATCACCGACG
AGTACAAGGT
GCCCAGCAAG
AAATTCAAGG
TGCTGGGCAA
CACCGACCGG
CACAGCATCA
AGAAGAACCT
GATCGGAGCC
CTGCTGTTCG
ACAGCGGCGA
AACAGCCGAG
GCCACCCGGC
TGAAGAGAAC
CGCCAGAAGA
AGATACACCA
GACGGAAGAA
CCGGATCTGC
TATCTGCAAG
AGATCTTCAG
CAACGAGATG
GCCAAGGTGG
ACGACAGCTT
CTTCCACAGA
CTGGAAGAGT
CCTTCCTGGT
GGAAGAGGAT
AAGAAGCACG
AGCGGCACCC
CATCTTCGGC
AACATCGTGG
ACGAGGTGGC
CTACCACGAG
AAGTACCCCA
CCATCTACCA
CCTGAGAAAG
AAACTGGTGG
ACAGCACCGA
CAAGGCCGAC
CTGCGGCTGA
TCTATCTGGC
CCTGGCCCAC
ATGATCAAGT
TCCGGGGCCA
CTTCCTGATC
GAGGGCGACC
TGAACCCCGA
CAACAGCGAC
GTGGACAAGC
TGTTCATCCA
GCTGGTGCAG
ACCTACAACC
AGCTGTTCGA
GGAAAACCCC
ATCAACGCCA
GCGGCGTGGA
CGCCAAGGCC
ATCCTGTCTG
CCAGACTGAG
CAAGAGCAGA
CGGCTGGAAA
ATCTGATCGC
CCAGCTGCCC
GGCGAGAAGA
AGAATGGCCT
GTTCGGAAAC
CTGATTGCCC
TGAGCCTGGG
CCTGACCCCC
AACTTCAAGA
GCAACTTCGA
CCTGGCCGAG
GATGCCAAAC
TGCAGCTGAG
CAAGGACACC
TACGACGACG
ACCTGGACAA
CCTGCTGGCC
CAGATCGGCG
ACCAGTACGC
CGACCTGTTT
CTGGCCGCCA
AGAACCTGTC
CGACGCCATC
CTGCTGAGCG
ACATCCTGAG
AGTGAACACC
GAGATCACCA
AGGCCCCCCT
GAGCGCCTCT
ATGATCAAGA
GATACGACGA
GCACCACCAG
GACCTGACCC
TGCTGAAAGC
TCTCGTGCGG
CAGCAGCTGC
CTGAGAAGTA
CAAAGAGATT
TTCTTCGACC
AGAGCAAGAA
CGGCTACGCC
GGCTACATTG
ACGGCGGAGC
CAGCCAGGAA
GAGTTCTACA
AGTTCATCAA
GCCCATCCTG
GAAAAGATGG
ACGGCACCGA
GGAACTGCTC
GTGAAGCTGA
ACAGAGAGGA
CCTGCTGCGG
AAGCAGCGGA
CCTTCGACAA
CGGCAGCATC
CCCCACCAGA
TCCACCTGGG
AGAGCTGCAC
GCCATTCTGC
GGCGGCAGGA
AGATTTTTAC
CCATTCCTGA
AGGACAACCG
GGAAAAGATC
GAGAAGATCC
TGACCTTCCG
CATCCCCTAC
TACGTGGGCC
CTCTGGCCAG
GGGAAACAGC
AGATTCGCCT
GGATGACCAG
AAAGAGCGAG
GAAACCATCA
CCCCCTGGAA
CTTCGAGGAA
GTGGTGGACA
AGGGCGCTTC
CGCCCAGAGC
TTCATCGAGC
GGATGACCAA
CTTCGATAAG
AACCTGCCCA
ACGAGAAGGT
GCTGCCCAAG
CACAGCCTGC
TGTACGAGTA
CTTCACCGTG
TATAACGAGC
TGACCAAAGT
GAAATACGTG
ACCGAGGGAA
TGAGAAAGCC
CGCCTTCCTG
AGCGGCGAGC
AGAAAAAGGC
CATCGTGGAC
CTGCTGTTCA
AGACCAACCG
GAAAGTGACC
GTGAAGCAGC
TGAAAGAGGA
CTACTTCAAG
AAAATCGAGT
GCTTCGACTC
CGTGGAAATC
TCCGGCGTGG
AAGATCGGTT
CAACGCCTCC
CTGGGCACAT
ACCACGATCT
GCTGAAAATT
ATCAAGGACA
AGGACTTCCT
GGACAATGAG
GAAAACGAGG
ACATTCTGGA
AGATATCGTG
CTGACCCTGA
CACTGTTTGA
GGACAGAGAG
ATGATCGAGG
AACGGCTGAA
AACCTATGCC
CACCTGTTCG
ACGACAAAGT
GATGAAGCAG
CTGAAGCGGC
GGAGATACAC
CGGCTGGGGC
AGGCTGAGCC
GGAAGCTGAT
CAACGGCATC
CGGGACAAGC
AGTCCGGCAA
GACAATCCTG
GATTTCCTGA
AGTCCGACGG
CTTCGCCAAC
AGAAACTTCA
TGCAGCTGAT
CCACGACGAC
AGCCTGACCT
TTAAAGAGGA
CATCCAGAAA
GCCCAGGTGT
CCGGCCAGGG
CGATAGCCTG
CACGAGCACA
TTGCCAATCT
GGCCGGCAGC
CCCGCCATTA
AGAAGGGCAT
CCTGCAGACA
GTGAAGGTGG
TGGACGAGCT
CGTGAAAGTG
ATGGGCCGGC
ACAAGCCCGA
GAACATCGTG
ATCGAAATGG
CCAGAGAGAA
CCAGACCACC
CAGAAGGGAC
AGAAGAACAG
CCGCGAGAGA
ATGAAGCGGA
TCGAAGAGGG
CATCAAAGAG
CTGGGCAGCC
AGATCCTGAA
AGAACACCCC
GTGGAAAACA
CCCAGCTGCA
GAACGAGAAG
CTGTACCTGT
ACTACCTGCA
GAATGGGCGG
GATATGTACG
TGGACCAGGA
ACTGGACATC
AACCGGCTGT
CCGACTACGA
TGTGGACCAT
ATCGTGCCTC
AGAGCTTTCT
GAAGGACGAC
TCCATCGACA
ACAAGGTGCT
GACCAGAAGC
GACAAGAACC
GGGGCAAGAG
CGACAACGTG
CCCTCCGAAG
AGGTCGTGAA
GAAGATGAAG
AACTACTGGC
GGCAGCTGCT
GAACGCCAAG
CTGATTACCC
AGAGAAAGTT
CGACAATCTG
ACCAAGGCCG
AGAGAGGCGG
CCTGAGCGAA
CTGGATAAGG
CCGGCTTCAT
CAAGAGACAG
CTGGTGGAAA
CCCGGCAGAT
CACAAAGCAC
GTGGCACAGA
TCCTGGACTC
CCGGATGAAC
ACTAAGTACG
ACGAGAATGA
CAAGCTGATC
CGGGAAGTGA
AAGTGATCAC
CCTGAAGTCC
AAGCTGGTGT
CCGATTTCCG
GAAGGATTTC
CAGTTTTACA
AAGTGCGCGA
GATCAACAAC
TACCACCACG
CCCACGACGC
CTACCTGAAC
GCCGTCGTGG
GAACCGCCCT
GATCAAAAAG
TACCCTAAGC
TGGAAAGCGA
GTTCGTGTAC
GGCGACTACA
AGGTGTACGA
CGTGCGGAAG
ATGATCGCCA
AGAGCGAGCA
GGAAATCGGC
AAGGCTACCG
CCAAGTACTT
CTTCTACAGC
AACATCATGA
ACTTTTTCAA
GACCGAGATT
ACCCTGGCCA
ACGGCGAGAT
CCGGAAGCGG
CCTCTGATCG
AGACAAACGG
CGAAACCGGG
GAGATCGTGT
GGGATAAGGG
CCGGGATTTT
GCCACCGTGC
GGAAAGTGCT
GAGCATGCCC
CAAGTGAATA
TCGTGAAAAA
GACCGAGGTG
CAGACAGGCG
GCTTCAGCAA
AGAGTCTATC
CTGCCCAAGA
GGAACAGCGA
TAAGCTGATC
GCCAGAAAGA
AGGACTGGGA
CCCTAAGAAG
TACGGCGGCT
TCGACAGCCC
CACCGTGGCC
TATTCTGTGC
TGGTGGTGGC
CAAAGTGGAA
AAGGGCAAGT
CCAAGAAACT
GAAGAGTGTG
AAAGAGCTGC
TGGGGATCAC
CATCATGGAA
AGAAGCAGCT
TCGAGAAGAA
TCCCATCGAC
TTTCTGGAAG
CCAAGGGCTA
CAAAGAAGTG
AAAAAGGACC
TGATCATCAA
GCTGCCTAAG
TACTCCCTGT
TCGAGCTGGA
AAACGGCCGG
AAGAGAATGC
TGGCCTCTGC
CGGCGAACTG
CAGAAGGGAA
ACGAACTGGC
CCTGCCCTCC
AAATATGTGA
ACTTCCTGTA
CCTGGCCAGC
CACTATGAGA
AGCTGAAGGG
CTCCCCCGAG
GATAATGAGC
AGAAACAGCT
GTTTGTGGAA
CAGCACAAGC
ACTACCTGGA
CGAGATCATC
GAGCAGATCA
GCGAGTTCTC
CAAGAGAGTG
ATCCTGGCCG
ACGCTAATCT
GGACAAAGTG
CTGTCCGCCT
ACAACAAGCA
CCGGGATAAG
CCCATCAGAG
AGCAGGCCGA
GAATATCATC
CACCTGTTTA
CCCTGACCAA
TCTGGGAGCC
CCTGCCGCCT
TCAAGTACTT
TGACACCACC
ATCGACCGGA
AGAGGTACAC
CAGCACCAAA
GAGGTGCTGG
ACGCCACCCT
GATCCACCAG
AGCATCACCG
GCCTGTACGA
GACACGGATC
GACCTGTCTC
AGCTGGGAGG
CGACAAAAGG
CCGGCGGCCA
CGAAAAAGGC
CGGCCAGGCA
AAAAAGAAAA
AGGAATTCGG
CAGTGGAGAG
GGCAGAGGAA
GTCTGCTAAC
ATGCGGTGAC
GTCGAGGAGA
ATCCTGGCCC
AATGACCGAG
TACAAGCCCA
CGGTGCGCCT
CGCCACCCGC
GACGACGTCC
CCAGGGCCGT
ACGCACCCTC
GCCGCCGCGT
TCGCCGACTA
CCCCGCCACG
CGCCACACCG
TCGATCCGGA
CCGCCACATC
GAGCGGGTCA
CCGAGCTGCA
AGAACTCTTC
CTCACGCGCG
TCGGGCTCGA
CATCGGCAAG
GTGTGGGTCG
CGGACGACGG
CGCCGCGGTG
GCGGTCTGGA
CCACGCCGGA
GAGCGTCGAA
GCGGGGGCGG
TGTTCGCCGA
GATCGGCCCG
CGCATGGCCG
AGTTGAGCGG
TTCCCGGCTG
GCCGCGCAGC
AACAGATGGA
AGGCCTCCTG
GCGCCGCACC
GGCCCAAGGA
GCCCGCGTGG
TTCCTGGCCA
CCGTCGGAGT
CTCGCCCGAC
CACCAGGGCA
AGGGTCTGGG
CAGCGCCGTC
GTGCTCCCCG
GAGTGGAGGC
GGCCGAGCGC
GCCGGGGTGC
CCGCCTTCCT
GGAGACCTCC
GCGCCCCGCA
ACCTCCCCTT
CTACGAGCGG
CTCGGCTTCA
CCGTCACCGC
CGACGTCGAG
GTGCCCGAAG
GACCGCGCAC
CTGGTGCATG
ACCCGCAAGC
CCGGTGCCTG
AGAATTCTAA
CTAGAGCTCG
CTGATCAGCC
TCGACTGTGC
CTTCTAGTTG
CCAGCCATCT
GTTGTTTGCC
CCTCCCCCGT
GCCTTCCTTG
ACCCTGGAAG
GTGCCACTCC
CACTGTCCTT
TCCTAATAAA
ATGAGGAAAT
TGCATCGCAT
TGTCTGAGTA
GGTGTCATTC
TATTCTGGGG
GGTGGGGTGG
GGCAGGACAG
CAAGGGGGAG
GATTGGGAAG
AGAATAGCAG
GCATGCTGGG
GAGCGGCCGC
AGGAACCCCT
AGTGATGGAG
TTGGCCACTC
CCTCTCTGCG
CGCTCGCTCG
CTCACTGAGG
CCGGGCGACC
AAAGGTCGCC
CGACGCCCGG
GCTTTGCCCG
GGCGGCCTCA
GTGAGCGAGC
GAGCGCGCAG
CTGCCTGCAG
GGGCGCCTGA
TGCGGTATTT
TCTCCTTACG
CATCTGTGCG
GTATTTCACA
CCGCATACGT
CAAAGCAACC
ATAGTACGCG
CCCTGTAGCG
GCGCATTAAG
CGCGGCGGGT
GTGGTGGTTA
CGCGCAGCGT
GACCGCTACA
CTTGCCAGCG
CCCTAGCGCC
CGCTCCTTTC
GCTTTCTTCC
CTTCCTTTCT
CGCCACGTTC
GCCGGCTTTC
CCCGTCAAGC
TCTAAATCGG
GGGCTCCCTT
TAGGGTTCCG
ATTTAGTGCT
TTACGGCACC
TCGACCCCAA
AAAACTTGAT
TTGGGTGATG
GTTCACGTAG
TGGGCCATCG
CCCTGATAGA
CGGTTTTTCG
CCCTTTGACG
TTGGAGTCCA
CGTTCTTTAA
TAGTGGACTC
TTGTTCCAAA
CTGGAACAAC
ACTCAACCCT
ATCTCGGGCT
ATTCTTTTGA
TTTATAAGGG
ATTTTGCCGA
TTTCGGCCTA
TTGGTTAAAA
AATGAGCTGA
TTTAACAAAA
ATTTAACGCG
AATTTTAACA
AAATATTAAC
GTTTACAATT
TTATGGTGCA
CTCTCAGTAC
AATCTGCTCT
GATGCCGCAT
AGTTAAGCCA
GCCCCGACAC
CCGCCAACAC
CCGCTGACGC
GCCCTGACGG
GCTTGTCTGC
TCCCGGCATC
CGCTTACAGA
CAAGCTGTGA
CCGTCTCCGG
GAGCTGCATG
TGTCAGAGGT
TTTCACCGTC
ATCACCGAAA
CGCGCGAGAC
GAAAGGGCCT
CGTGATACGC
CTATTTTTAT
AGGTTAATGT
CATGATAATA
ATGGTTTCTT
AGACGTCAGG
TGGCACTTTT
CGGGGAAATG
TGCGCGGAAC
CCCTATTTGT
TTATTTTTCT
AAATACATTC
AAATATGTAT
CCGCTCATGA
GACAATAACC
CTGATAAATG
CTTCAATAAT
ATTGAAAAAG
GAAGAGTATG
AGTATTCAAC
ATTTCCGTGT
CGCCCTTATT
CCCTTTTTTG
CGGCATTTTG
CCTTCCTGTT
TTTGCTCACC
CAGAAACGCT
GGTGAAAGTA
AAAGATGCTG
AAGATCAGTT
GGGTGCACGA
GTGGGTTACA
TCGAACTGGA
TCTCAACAGC
GGTAAGATCC
TTGAGAGTTT
TCGCCCCGAA
GAACGTTTTC
CAATGATGAG
CACTTTTAAA
GTTCTGCTAT
GTGGCGCGGT
ATTATCCCGT
ATTGACGCCG
GGCAAGAGCA
ACTCGGTCGC
CGCATACACT
ATTCTCAGAA
TGACTTGGTT
GAGTACTCAC
CAGTCACAGA
AAAGCATCTT
ACGGATGGCA
TGACAGTAAG
AGAATTATGC
AGTGCTGCCA
TAACCATGAG
TGATAACACT
GCGGCCAACT
TACTTCTGAC
AACGATCGGA
GGACCGAAGG
AGCTAACCGC
TTTTTTGCAC
AACATGGGGG
ATCATGTAAC
TCGCCTTGAT
CGTTGGGAAC
CGGAGCTGAA
TGAAGCCATA
CCAAACGACG
AGCGTGACAC
CACGATGCCT
GTAGCAATGG
CAACAACGTT
GCGCAAACTA
TTAACTGGCG
AACTACTTAC
TCTAGCTTCC
CGGCAACAAT
TAATAGACTG
GATGGAGGCG
GATAAAGTTG
CAGGACCACT
TCTGCGCTCG
GCCCTTCCGG
CTGGCTGGTT
TATTGCTGAT
AAATCTGGAG
CCGGTGAGCG
TGGAAGCCGC
GGTATCATTG
CAGCACTGGG
GCCAGATGGT
AAGCCCTCCC
GTATCGTAGT
TATCTACACG
ACGGGGAGTC
AGGCAACTAT
GGATGAACGA
AATAGACAGA
TCGCTGAGAT
AGGTGCCTCA
CTGATTAAGC
ATTGGTAACT
GTCAGACCAA
GTTTACTCAT
ATATACTTTA
GATTGATTTA
AAACTTCATT
TTTAATTTAA
AAGGATCTAG
GTGAAGATCC
TTTTTGATAA
TCTCATGACC
AAAATCCCTT
AACGTGAGTT
TTCGTTCCAC
TGAGCGTCAG
ACCCCGTAGA
AAAGATCAAA
GGATCTTCTT
GAGATCCTTT
TTTTCTGCGC
GTAATCTGCT
GCTTGCAAAC
AAAAAAACCA
CCGCTACCAG
CGGTGGTTTG
TTTGCCGGAT
CAAGAGCTAC
CAACTCTTTT
TCCGAAGGTA
ACTGGCTTCA
GCAGAGCGCA
GATACCAAAT
ACTGTCCTTC
TAGTGTAGCC
GTAGTTAGGC
CACCACTTCA
AGAACTCTGT
AGCACCGCCT
ACATACCTCG
CTCTGCTAAT
CCTGTTACCA
GTGGCTGCTG
CCAGTGGCGA
TAAGTCGTGT
CTTACCGGGT
TGGACTCAAG
ACGATAGTTA
CCGGATAAGG
CGCAGCGGTC
GGGCTGAACG
GGGGGTTCGT
GCACACAGCC
CAGCTTGGAG
CGAACGACCT
ACACCGAACT
GAGATACCTA
CAGCGTGAGC
TATGAGAAAG
CGCCACGCTT
CCCGAAGGGA
GAAAGGCGGA
CAGGTATCCG
GTAAGCGGCA
GGGTCGGAAC
AGGAGAGCGC
ACGAGGGAGC
TTCCAGGGGG
AAACGCCTGG
TATCTTTATA
GTCCTGTCGG
GTTTCGCCAC
CTCTGACTTG
AGCGTCGATT
TTTGTGATGC
TCGTCAGGGG
GGCGGAGCCT
ATGGAAAAAC
GCCAGCAACG
CGGCCTTTTT
ACGGTTCCTG
GCCTTTTGCT
GGCCTTTTGC
TCACATGT
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