名称: | pLentiV2-dCas9-VP64 |
---|---|
别称: | Plasmid #141104 |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 空载体 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | EF1a,U6 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | LentiCRISPR V2 |
正向引物: | G35310-F1:AGGTACCTGAGGTGTGACTG |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTCGACGGAT
CGGGAGATCT
CCCGATCCCC
TATGGTGCAC
TCTCAGTACA
ATCTGCTCTG
ATGCCGCATA
GTTAAGCCAG
TATCTGCTCC
CTGCTTGTGT
GTTGGAGGTC
GCTGAGTAGT
GCGCGAGCAA
AATTTAAGCT
ACAACAAGGC
AAGGCTTGAC
CGACAATTGC
ATGAAGAATC
TGCTTAGGGT
TAGGCGTTTT
GCGCTGCTTC
GCGATGTACG
GGCCAGATAT
ACGCGTTGAC
ATTGATTATT
GACTAGTTAT
TAATAGTAAT
CAATTACGGG
GTCATTAGTT
CATAGCCCAT
ATATGGAGTT
CCGCGTTACA
TAACTTACGG
TAAATGGCCC
GCCTGGCTGA
CCGCCCAACG
ACCCCCGCCC
ATTGACGTCA
ATAATGACGT
ATGTTCCCAT
AGTAACGCCA
ATAGGGACTT
TCCATTGACG
TCAATGGGTG
GAGTATTTAC
GGTAAACTGC
CCACTTGGCA
GTACATCAAG
TGTATCATAT
GCCAAGTACG
CCCCCTATTG
ACGTCAATGA
CGGTAAATGG
CCCGCCTGGC
ATTATGCCCA
GTACATGACC
TTATGGGACT
TTCCTACTTG
GCAGTACATC
TACGTATTAG
TCATCGCTAT
TACCATGGTG
ATGCGGTTTT
GGCAGTACAT
CAATGGGCGT
GGATAGCGGT
TTGACTCACG
GGGATTTCCA
AGTCTCCACC
CCATTGACGT
CAATGGGAGT
TTGTTTTGGC
ACCAAAATCA
ACGGGACTTT
CCAAAATGTC
GTAACAACTC
CGCCCCATTG
ACGCAAATGG
GCGGTAGGCG
TGTACGGTGG
GAGGTCTATA
TAAGCAGCGC
GTTTTGCCTG
TACTGGGTCT
CTCTGGTTAG
ACCAGATCTG
AGCCTGGGAG
CTCTCTGGCT
AACTAGGGAA
CCCACTGCTT
AAGCCTCAAT
AAAGCTTGCC
TTGAGTGCTT
CAAGTAGTGT
GTGCCCGTCT
GTTGTGTGAC
TCTGGTAACT
AGAGATCCCT
CAGACCCTTT
TAGTCAGTGT
GGAAAATCTC
TAGCAGTGGC
GCCCGAACAG
GGACTTGAAA
GCGAAAGGGA
AACCAGAGGA
GCTCTCTCGA
CGCAGGACTC
GGCTTGCTGA
AGCGCGCACG
GCAAGAGGCG
AGGGGCGGCG
ACTGGTGAGT
ACGCCAAAAA
TTTTGACTAG
CGGAGGCTAG
AAGGAGAGAG
ATGGGTGCGA
GAGCGTCAGT
ATTAAGCGGG
GGAGAATTAG
ATCGCGATGG
GAAAAAATTC
GGTTAAGGCC
AGGGGGAAAG
AAAAAATATA
AATTAAAACA
TATAGTATGG
GCAAGCAGGG
AGCTAGAACG
ATTCGCAGTT
AATCCTGGCC
TGTTAGAAAC
ATCAGAAGGC
TGTAGACAAA
TACTGGGACA
GCTACAACCA
TCCCTTCAGA
CAGGATCAGA
AGAACTTAGA
TCATTATATA
ATACAGTAGC
AACCCTCTAT
TGTGTGCATC
AAAGGATAGA
GATAAAAGAC
ACCAAGGAAG
CTTTAGACAA
GATAGAGGAA
GAGCAAAACA
AAAGTAAGAC
CACCGCACAG
CAAGCGGCCG
CTGATCTTCA
GACCTGGAGG
AGGAGATATG
AGGGACAATT
GGAGAAGTGA
ATTATATAAA
TATAAAGTAG
TAAAAATTGA
ACCATTAGGA
GTAGCACCCA
CCAAGGCAAA
GAGAAGAGTG
GTGCAGAGAG
AAAAAAGAGC
AGTGGGAATA
GGAGCTTTGT
TCCTTGGGTT
CTTGGGAGCA
GCAGGAAGCA
CTATGGGCGC
AGCGTCAATG
ACGCTGACGG
TACAGGCCAG
ACAATTATTG
TCTGGTATAG
TGCAGCAGCA
GAACAATTTG
CTGAGGGCTA
TTGAGGCGCA
ACAGCATCTG
TTGCAACTCA
CAGTCTGGGG
CATCAAGCAG
CTCCAGGCAA
GAATCCTGGC
TGTGGAAAGA
TACCTAAAGG
ATCAACAGCT
CCTGGGGATT
TGGGGTTGCT
CTGGAAAACT
CATTTGCACC
ACTGCTGTGC
CTTGGAATGC
TAGTTGGAGT
AATAAATCTC
TGGAACAGAT
TTGGAATCAC
ACGACCTGGA
TGGAGTGGGA
CAGAGAAATT
AACAATTACA
CAAGCTTAAT
ACACTCCTTA
ATTGAAGAAT
CGCAAAACCA
GCAAGAAAAG
AATGAACAAG
AATTATTGGA
ATTAGATAAA
TGGGCAAGTT
TGTGGAATTG
GTTTAACATA
ACAAATTGGC
TGTGGTATAT
AAAATTATTC
ATAATGATAG
TAGGAGGCTT
GGTAGGTTTA
AGAATAGTTT
TTGCTGTACT
TTCTATAGTG
AATAGAGTTA
GGCAGGGATA
TTCACCATTA
TCGTTTCAGA
CCCACCTCCC
AACCCCGAGG
GGACCCGACA
GGCCCGAAGG
AATAGAAGAA
GAAGGTGGAG
AGAGAGACAG
AGACAGATCC
ATTCGATTAG
TGAACGGATC
GGCACTGCGT
GCGCCAATTC
TGCAGACAAA
TGGCAGTATT
CATCCACAAT
TTTAAAAGAA
AAGGGGGGAT
TGGGGGGTAC
AGTGCAGGGG
AAAGAATAGT
AGACATAATA
GCAACAGACA
TACAAACTAA
AGAATTACAA
AAACAAATTA
CAAAAATTCA
AAATTTTCGG
GTTTATTACA
GGGACAGCAG
AGATCCAGTT
TGGTTAATTA
AGGTACCGAG
GGCCTATTTC
CCATGATTCC
TTCATATTTG
CATATACGAT
ACAAGGCTGT
TAGAGAGATA
ATTAGAATTA
ATTTGACTGT
AAACACAAAG
ATATTAGTAC
AAAATACGTG
ACGTAGAAAG
TAATAATTTC
TTGGGTAGTT
TGCAGTTTTA
AAATTATGTT
TTAAAATGGA
CTATCATATG
CTTACCGTAA
CTTGAAAGTA
TTTCGATTTC
TTGGCTTTAT
ATATCTTGTG
GAAAGGACGA
AACACCGGAG
ACGGTTGTAA
ATGAGCACAC
AAAATACACA
TGCTAAAATA
TTATATTCTA
TGACCTTTAT
AAAATCAACC
AAAATCTTCT
TTTTAATAAC
TTTAGTATCA
ATAATTAGAA
TTTTTATGTT
CCTTTTTGCA
AACTTTTAAT
AAAAATGAGC
AAAATAAAAA
AACGCTAGTT
TTAGTAACTC
GCGTTGTTTT
CTTCACCTTT
AATAATAGCT
ACTCCACCAC
TTGTTCCTAA
GCGGTCAGCT
CCTGCTTCAA
TCATTTTTTG
AGCATCTTCA
AATGTTCTAA
CTCCACCAGC
TGCTTTAACT
AAAGCATTGT
CTTTAACAAC
TGACTTCATT
AGTTTAACAT
CTTCAAATGT
TGCACCTGAT
TTTGAAAATC
CTGTTGATGT
TTTAACAAAT
TCTAATCCAG
CTTCAACAGC
TATTTCACAA
GCTTTCATGA
TTTCTTCTTT
TGTTAATAAA
CAATTTTCCA
TAATACATTT
AACAACATGT
GATCCAGCTG
CTTTTTTTAC
AGCTTTCATG
TCTTCTAAAA
CTAATTCATA
ATTTTTGTCT
TTTAATGCAC
CAATATTTAA
TACCATATCA
ATTTCTGTTG
CACCATCTTT
AATTGCTTCA
GAAACTTCGA
ATGCTTTTGT
AGCTGTTGTG
CATGCACCTA
GAGGAAAACC
TACAACATTT
GTTATTCCTA
CATTTGTGCC
TTTTAATAAT
TCTTTACAAT
AGCTTGTTCA
ATATGAATTA
ACACAAACTG
TTGCAAAATC
AAATTCAATT
GCTTCATCAC
ATAATTGTTT
AATTTCAGCT
TTCGTAGCAT
CTTGTTTTAA
TAATGTGTGA
TCTATATATT
TGTTTAGTTT
CATTTTTTCT
CCTATATATT
CATTTTTAAT
TTTAATTCTT
TAATAATTTC
GTCTACTTTA
ACTTTAGCGT
TTTGAACAGA
TTCACCAACA
CCTATAAAAT
AAATTTTTAG
TTTAGGTTCA
GTTCCACTTG
GGCGAACAGC
AAATCATGAC
TTATCTTCTA
AATAAAATTT
TAGTAAGTCT
TGTCCTGGCA
TATTATACAT
TCCATCGATG
TAGTCTTCAA
CATTAACAAC
TTTAAGTCCA
GCAATTTGAG
TTAAGGGTGT
TGCTCTCAAT
GATTTCATTA
ATGGTTCAAT
TTTTAATTTC
TTTTCTTCTG
GTTTAAAATT
CAAGTTTAAA
GTGAAAGTGT
AATATGCACC
CATTTCTTTA
AATAAATCTT
CTAAATAGTC
TACTAATGTT
TTATTTTGTT
TTTTATAAAA
TCAAGCAGCC
TCTGCTATTA
ATATAGAAGC
TTGTATTCCA
TCTTTATCTC
TAGCTGAGTC
ATCAATTACA
TATCCATAAC
TTTCTTCATA
AGCAAAAACA
AAATTTAATC
CGTTATCTTC
TTCTTTAGCA
ATTTCTCTAC
CCATTCATTT
AAATCCAGTT
AAAGTTTTTA
CAATATTAAC
TCCATATTTT
TCATGAGCGA
TTCTATCACC
CAAATCACTT
GTTACAAAAC
TTGAATATAG
AGCCGGATTT
TTTGGAATGC
TATTTAAGCG
TTTTAGATTT
GATAATTTTC
AATCAATTAA
AATTGGTCCT
GTTTGATTTC
CATCTAATCT
TACAAAATGA
CCATCATGTT
TTATTGCCAT
TCCAAATCTG
TCAGCATCTG
GGTCATTCAT
AATAATAATA
TCTGCATCAT
GTTTAATACC
ATATTCAAGC
GGTATTTTTC
ATGCAGGATC
AAATTCTGGA
TTTGGATTTA
CAACATTTTT
AAATGTTTCA
TCTTCAAATG
CATGCTCTTC
AACCTCAATA
ACGTTATATC
CTGATTCACG
TAATATTTTT
GGGGTAAATT
TAGTTCCTGT
TCCATTAACT
GCGCTAAAAA
TAATTTTTAA
ATCTTTTTTA
GCTTCTTGCT
CTTTTTTGTA
CGTCTCTGTT
TTAGAGCTAG
AAATAGCAAG
TTAAAATAAG
GCTAGTCCGT
TATCAACTTG
AAAAAGTGGC
ACCGAGTCGG
TGCTTTTTTG
AATTCGCTAG
CTAGGTCTTG
AAAGGAGTGG
GAATTGGCTC
CGGTGCCCGT
CAGTGGGCAG
AGCGCACATC
GCCCACAGTC
CCCGAGAAGT
TGGGGGGAGG
GGTCGGCAAT
TGATCCGGTG
CCTAGAGAAG
GTGGCGCGGG
GTAAACTGGG
AAAGTGATGT
CGTGTACTGG
CTCCGCCTTT
TTCCCGAGGG
TGGGGGAGAA
CCGTATATAA
GTGCAGTAGT
CGCCGTGAAC
GTTCTTTTTC
GCAACGGGTT
TGCCGCCAGA
ACACAGGCGT
ACGGCCACCA
TGAAAAGGCC
GGCGGCCACG
AAAAAGGCCG
GCCAGGCAAA
AAAGAAAAAG
GACAAGAAGT
ACAGCATCGG
CCTGGCCATC
GGCACCAACT
CTGTGGGCTG
GGCCGTGATC
ACCGACGAGT
ACAAGGTGCC
CAGCAAGAAA
TTCAAGGTGC
TGGGCAACAC
CGACCGGCAC
AGCATCAAGA
AGAACCTGAT
CGGAGCCCTG
CTGTTCGACA
GCGGCGAAAC
AGCCGAGGCC
ACCCGGCTGA
AGAGAACCGC
CAGAAGAAGA
TACACCAGAC
GGAAGAACCG
GATCTGCTAT
CTGCAAGAGA
TCTTCAGCAA
CGAGATGGCC
AAGGTGGACG
ACAGCTTCTT
CCACAGACTG
GAAGAGTCCT
TCCTGGTGGA
AGAGGATAAG
AAGCACGAGC
GGCACCCCAT
CTTCGGCAAC
ATCGTGGACG
AGGTGGCCTA
CCACGAGAAG
TACCCCACCA
TCTACCACCT
GAGAAAGAAA
CTGGTGGACA
GCACCGACAA
GGCCGACCTG
CGGCTGATCT
ATCTGGCCCT
GGCCCACATG
ATCAAGTTCC
GGGGCCACTT
CCTGATCGAG
GGCGACCTGA
ACCCCGACAA
CAGCGACGTG
GACAAGCTGT
TCATCCAGCT
GGTGCAGACC
TACAACCAGC
TGTTCGAGGA
AAACCCCATC
AACGCCAGCG
GCGTGGACGC
CAAGGCCATC
CTGTCTGCCA
GACTGAGCAA
GAGCAGACGG
CTGGAAAATC
TGATCGCCCA
GCTGCCCGGC
GAGAAGAAGA
ATGGCCTGTT
CGGCAACCTG
ATTGCCCTGA
GCCTGGGCCT
GACCCCCAAC
TTCAAGAGCA
ACTTCGACCT
GGCCGAGGAT
GCCAAACTGC
AGCTGAGCAA
GGACACCTAC
GACGACGACC
TGGACAACCT
GCTGGCCCAG
ATCGGCGACC
AGTACGCCGA
CCTGTTTCTG
GCCGCCAAGA
ACCTGTCCGA
CGCCATCCTG
CTGAGCGACA
TCCTGAGAGT
GAACACCGAG
ATCACCAAGG
CCCCCCTGAG
CGCCTCTATG
ATCAAGAGAT
ACGACGAGCA
CCACCAGGAC
CTGACCCTGC
TGAAAGCTCT
CGTGCGGCAG
CAGCTGCCTG
AGAAGTACAA
AGAGATTTTC
TTCGACCAGA
GCAAGAACGG
CTACGCCGGC
TACATTGACG
GCGGAGCCAG
CCAGGAAGAG
TTCTACAAGT
TCATCAAGCC
CATCCTGGAA
AAGATGGACG
GCACCGAGGA
ACTGCTCGTG
AAGCTGAACA
GAGAGGACCT
GCTGCGGAAG
CAGCGGACCT
TCGACAACGG
CAGCATCCCC
CACCAGATCC
ACCTGGGAGA
GCTGCACGCC
ATTCTGCGGC
GGCAGGAAGA
TTTTTACCCA
TTCCTGAAGG
ACAACCGGGA
AAAGATCGAG
AAGATCCTGA
CCTTCCGCAT
CCCCTACTAC
GTGGGCCCTC
TGGCCAGGGG
AAACAGCAGA
TTCGCCTGGA
TGACCAGAAA
GAGCGAGGAA
ACCATCACCC
CCTGGAACTT
CGAGGAAGTG
GTGGACAAGG
GCGCTTCCGC
CCAGAGCTTC
ATCGAGCGGA
TGACCAACTT
CGATAAGAAC
CTGCCCAACG
AGAAGGTGCT
GCCCAAGCAC
AGCCTGCTGT
ACGAGTACTT
CACCGTGTAT
AACGAGCTGA
CCAAAGTGAA
ATACGTGACC
GAGGGAATGA
GAAAGCCCGC
CTTCCTGAGC
GGCGAGCAGA
AAAAGGCCAT
CGTGGACCTG
CTGTTCAAGA
CCAACCGGAA
AGTGACCGTG
AAGCAGCTGA
AAGAGGACTA
CTTCAAGAAA
ATCGAGTGCT
TCGACTCCGT
GGAAATCTCC
GGCGTGGAAG
ATCGGTTCAA
CGCCTCCCTG
GGCACATACC
ACGATCTGCT
GAAAATTATC
AAGGACAAGG
ACTTCCTGGA
CAATGAGGAA
AACGAGGACA
TTCTGGAAGA
TATCGTGCTG
ACCCTGACAC
TGTTTGAGGA
CAGAGAGATG
ATCGAGGAAC
GGCTGAAAAC
CTATGCCCAC
CTGTTCGACG
ACAAAGTGAT
GAAGCAGCTG
AAGCGGCGGA
GATACACCGG
CTGGGGCAGG
CTGAGCCGGA
AGCTGATCAA
CGGCATCCGG
GACAAGCAGT
CCGGCAAGAC
AATCCTGGAT
TTCCTGAAGT
CCGACGGCTT
CGCCAACAGA
AACTTCATGC
AGCTGATCCA
CGACGACAGC
CTGACCTTTA
AAGAGGACAT
CCAGAAAGCC
CAGGTGTCCG
GCCAGGGCGA
TAGCCTGCAC
GAGCACATTG
CCAATCTGGC
CGGCAGCCCC
GCCATTAAGA
AGGGCATCCT
GCAGACAGTG
AAGGTGGTGG
ACGAGCTCGT
GAAAGTGATG
GGCCGGCACA
AGCCCGAGAA
CATCGTGATC
GAAATGGCCA
GAGAGAACCA
GACCACCCAG
AAGGGACAGA
AGAACAGCCG
CGAGAGAATG
AAGCGGATCG
AAGAGGGCAT
CAAAGAGCTG
GGCAGCCAGA
TCCTGAAAGA
ACACCCCGTG
GAAAACACCC
AGCTGCAGAA
CGAGAAGCTG
TACCTGTACT
ACCTGCAGAA
TGGGCGGGAT
ATGTACGTGG
ACCAGGAACT
GGACATCAAC
CGGCTGTCCG
ACTACGATGT
GGACCACATC
GTGCCTCAGA
GCTTTCTGAA
GGACGACTCC
ATCGACAACA
AGGTGCTGAC
CAGAAGCGAC
AAGGCCCGGG
GCAAGAGCGA
CAACGTGCCC
TCCGAAGAGG
TCGTGAAGAA
GATGAAGAAC
TACTGGCGGC
AGCTGCTGAA
CGCCAAGCTG
ATTACCCAGA
GAAAGTTCGA
CAATCTGACC
AAGGCCGAGA
GAGGCGGCCT
GAGCGAACTG
GATAAGGCCG
GCTTCATCAA
GAGACAGCTG
GTGGAAACCC
GGCAGATCAC
AAAGCACGTG
GCACAGATCC
TGGACTCCCG
GATGAACACT
AAGTACGACG
AGAATGACAA
GCTGATCCGG
GAAGTGAAAG
TGATCACCCT
GAAGTCCAAG
CTGGTGTCCG
ATTTCCGGAA
GGATTTCCAG
TTTTACAAAG
TGCGCGAGAT
CAACAACTAC
CACCACGCCC
ACGACGCCTA
CCTGAACGCC
GTCGTGGGAA
CCGCCCTGAT
CAAAAAGTAC
CCTAAGCTGG
AAAGCGAGTT
CGTGTACGGC
GACTACAAGG
TGTACGACGT
GCGGAAGATG
ATCGCCAAGA
GCGAGCAGGA
AATCGGCAAG
GCTACCGCCA
AGTACTTCTT
CTACAGCAAC
ATCATGAACT
TTTTCAAGAC
CGAGATTACC
CTGGCCAACG
GCGAGATCCG
GAAGCGGCCT
CTGATCGAGA
CAAACGGCGA
AACCGGGGAG
ATCGTGTGGG
ATAAGGGCCG
GGATTTTGCC
ACCGTGCGGA
AAGTGCTGAG
CATGCCCCAA
GTGAATATCG
TGAAAAAGAC
CGAGGTGCAG
ACAGGCGGCT
TCAGCAAAGA
GTCTATCCTG
CCCAAGAGGA
ACAGCGATAA
GCTGATCGCC
AGAAAGAAGG
ACTGGGACCC
TAAGAAGTAC
GGCGGCTTCG
ACAGCCCCAC
CGTGGCCTAT
TCTGTGCTGG
TGGTGGCCAA
AGTGGAAAAG
GGCAAGTCCA
AGAAACTGAA
GAGTGTGAAA
GAGCTGCTGG
GGATCACCAT
CATGGAAAGA
AGCAGCTTCG
AGAAGAATCC
CATCGACTTT
CTGGAAGCCA
AGGGCTACAA
AGAAGTGAAA
AAGGACCTGA
TCATCAAGCT
GCCTAAGTAC
TCCCTGTTCG
AGCTGGAAAA
CGGCCGGAAG
AGAATGCTGG
CCTCTGCCGG
CGAACTGCAG
AAGGGAAACG
AACTGGCCCT
GCCCTCCAAA
TATGTGAACT
TCCTGTACCT
GGCCAGCCAC
TATGAGAAGC
TGAAGGGCTC
CCCCGAGGAT
AATGAGCAGA
AACAGCTGTT
TGTGGAACAG
CACAAGCACT
ACCTGGACGA
GATCATCGAG
CAGATCAGCG
AGTTCTCCAA
GAGAGTGATC
CTGGCCGACG
CTAATCTGGA
CAAAGTGCTG
TCCGCCTACA
ACAAGCACCG
GGATAAGCCC
ATCAGAGAGC
AGGCCGAGAA
TATCATCCAC
CTGTTTACCC
TGACCAATCT
GGGAGCCCCT
GCCGCCTTCA
AGTACTTTGA
CACCACCATC
GACCGGAAGA
GGTACACCAG
CACCAAAGAG
GTGCTGGACG
CCACCCTGAT
CCACCAGAGC
ATCACCGGCC
TGTACGAGAC
ACGGATCGAC
CTGTCTCAGC
TGGGAGGCGA
CAGCGCTGGA
GGAGGTGGAA
GCGGAGGAGG
AGGAAGCGGA
GGAGGAGGTA
GCGGACCTAA
GAAAAAGAGG
AAGGTGGCGG
CCGCTGGATC
CGGACGGGCT
GACGCATTGG
ACGATTTTGA
TCTGGATATG
CTGGGAAGTG
ACGCCCTCGA
TGATTTTGAC
CTTGACATGC
TTGGTTCGGA
TGCCCTTGAT
GACTTTGACC
TCGACATGCT
CGGCAGTGAC
GCCCTTGATG
ATTTCGACCT
GGACATGCTG
ATTAACTGTA
CAGGCAGTGG
AGCAACAAAC
TTCTCTCTGC
TGAAACAAGC
CGGAGATGTC
GAAGAGAATC
CTGGACCGAC
CGAGTACAAG
CCCACGGTGC
GCCTCGCCAC
CCGCGACGAC
GTCCCCAGGG
CCGTACGCAC
CCTCGCCGCC
GCGTTCGCCG
ACTACCCCGC
CACGCGCCAC
ACCGTCGATC
CGGACCGCCA
CATCGAGCGG
GTCACCGAGC
TGCAAGAACT
CTTCCTCACG
CGCGTCGGGC
TCGACATCGG
CAAGGTGTGG
GTCGCGGACG
ACGGCGCCGC
GGTGGCGGTC
TGGACCACGC
CGGAGAGCGT
CGAAGCGGGG
GCGGTGTTCG
CCGAGATCGG
CCCGCGCATG
GCCGAGTTGA
GCGGTTCCCG
GCTGGCCGCG
CAGCAACAGA
TGGAAGGCCT
CCTGGCGCCG
CACCGGCCCA
AGGAGCCCGC
GTGGTTCCTG
GCCACCGTCG
GAGTCTCGCC
CGACCACCAG
GGCAAGGGTC
TGGGCAGCGC
CGTCGTGCTC
CCCGGAGTGG
AGGCGGCCGA
GCGCGCCGGG
GTGCCCGCCT
TCCTGGAGAC
CTCCGCGCCC
CGCAACCTCC
CCTTCTACGA
GCGGCTCGGC
TTCACCGTCA
CCGCCGACGT
CGAGGTGCCC
GAAGGACCGC
GCACCTGGTG
CATGACCCGC
AAGCCCGGTG
CCTGAACGCG
TTAAGTCGAC
AATCAACCTC
TGGATTACAA
AATTTGTGAA
AGATTGACTG
GTATTCTTAA
CTATGTTGCT
CCTTTTACGC
TATGTGGATA
CGCTGCTTTA
ATGCCTTTGT
ATCATGCTAT
TGCTTCCCGT
ATGGCTTTCA
TTTTCTCCTC
CTTGTATAAA
TCCTGGTTGC
TGTCTCTTTA
TGAGGAGTTG
TGGCCCGTTG
TCAGGCAACG
TGGCGTGGTG
TGCACTGTGT
TTGCTGACGC
AACCCCCACT
GGTTGGGGCA
TTGCCACCAC
CTGTCAGCTC
CTTTCCGGGA
CTTTCGCTTT
CCCCCTCCCT
ATTGCCACGG
CGGAACTCAT
CGCCGCCTGC
CTTGCCCGCT
GCTGGACAGG
GGCTCGGCTG
TTGGGCACTG
ACAATTCCGT
GGTGTTGTCG
GGGAAATCAT
CGTCCTTTCC
TTGGCTGCTC
GCCTGTGTTG
CCACCTGGAT
TCTGCGCGGG
ACGTCCTTCT
GCTACGTCCC
TTCGGCCCTC
AATCCAGCGG
ACCTTCCTTC
CCGCGGCCTG
CTGCCGGCTC
TGCGGCCTCT
TCCGCGTCTT
CGCCTTCGCC
CTCAGACGAG
TCGGATCTCC
CTTTGGGCCG
CCTCCCCGCA
TCGATACCGT
CGACCTCGAG
ACCTAGAAAA
ACATGGAGCA
ATCACAAGTA
GCAATACAGC
AGCTACCAAT
GCTGATTGTG
CCTGGCTAGA
AGCACAAGAG
GAGGAGGAGG
TGGGTTTTCC
AGTCACACCT
CAGGTACCTT
TAAGACCAAT
GACTTACAAG
GCAGCTGTAG
ATCTTAGCCA
CTTTTTAAAA
GAAAAGGGGG
GACTGGAAGG
GCTAATTCAC
TCCCAACGAA
GACAAGATAT
CCTTGATCTG
TGGATCTACC
ACACACAAGG
CTACTTCCCT
GATTGGCAGA
ACTACACACC
AGGGCCAGGG
ATCAGATATC
CACTGACCTT
TGGATGGTGC
TACAAGCTAG
TACCAGTTGA
GCAAGAGAAG
GTAGAAGAAG
CCAATGAAGG
AGAGAACACC
CGCTTGTTAC
ACCCTGTGAG
CCTGCATGGG
ATGGATGACC
CGGAGAGAGA
AGTATTAGAG
TGGAGGTTTG
ACAGCCGCCT
AGCATTTCAT
CACATGGCCC
GAGAGCTGCA
TCCGGACTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGGGCCC
GTTTAAACCC
GCTGATCAGC
CTCGACTGTG
CCTTCTAGTT
GCCAGCCATC
TGTTGTTTGC
CCCTCCCCCG
TGCCTTCCTT
GACCCTGGAA
GGTGCCACTC
CCACTGTCCT
TTCCTAATAA
AATGAGGAAA
TTGCATCGCA
TTGTCTGAGT
AGGTGTCATT
CTATTCTGGG
GGGTGGGGTG
GGGCAGGACA
GCAAGGGGGA
GGATTGGGAA
GACAATAGCA
GGCATGCTGG
GGATGCGGTG
GGCTCTATGG
CTTCTGAGGC
GGAAAGAACC
AGCTGGGGCT
CTAGGGGGTA
TCCCCACGCG
CCCTGTAGCG
GCGCATTAAG
CGCGGCGGGT
GTGGTGGTTA
CGCGCAGCGT
GACCGCTACA
CTTGCCAGCG
CCCTAGCGCC
CGCTCCTTTC
GCTTTCTTCC
CTTCCTTTCT
CGCCACGTTC
GCCGGCTTTC
CCCGTCAAGC
TCTAAATCGG
GGGCTCCCTT
TAGGGTTCCG
ATTTAGTGCT
TTACGGCACC
TCGACCCCAA
AAAACTTGAT
TAGGGTGATG
GTTCACGTAG
TGGGCCATCG
CCCTGATAGA
CGGTTTTTCG
CCCTTTGACG
TTGGAGTCCA
CGTTCTTTAA
TAGTGGACTC
TTGTTCCAAA
CTGGAACAAC
ACTCAACCCT
ATCTCGGTCT
ATTCTTTTGA
TTTATAAGGG
ATTTTGCCGA
TTTCGGCCTA
TTGGTTAAAA
AATGAGCTGA
TTTAACAAAA
ATTTAACGCG
AATTAATTCT
GTGGAATGTG
TGTCAGTTAG
GGTGTGGAAA
GTCCCCAGGC
TCCCCAGCAG
GCAGAAGTAT
GCAAAGCATG
CATCTCAATT
AGTCAGCAAC
CAGGTGTGGA
AAGTCCCCAG
GCTCCCCAGC
AGGCAGAAGT
ATGCAAAGCA
TGCATCTCAA
TTAGTCAGCA
ACCATAGTCC
CGCCCCTAAC
TCCGCCCATC
CCGCCCCTAA
CTCCGCCCAG
TTCCGCCCAT
TCTCCGCCCC
ATGGCTGACT
AATTTTTTTT
ATTTATGCAG
AGGCCGAGGC
CGCCTCTGCC
TCTGAGCTAT
TCCAGAAGTA
GTGAGGAGGC
TTTTTTGGAG
GCCTAGGCTT
TTGCAAAAAG
CTCCCGGGAG
CTTGTATATC
CATTTTCGGA
TCTGATCAGC
ACGTGTTGAC
AATTAATCAT
CGGCATAGTA
TATCGGCATA
GTATAATACG
ACAAGGTGAG
GAACTAAACC
ATGGCCAAGT
TGACCAGTGC
CGTTCCGGTG
CTCACCGCGC
GCGACGTCGC
CGGAGCGGTC
GAGTTCTGGA
CCGACCGGCT
CGGGTTCTCC
CGGGACTTCG
TGGAGGACGA
CTTCGCCGGT
GTGGTCCGGG
ACGACGTGAC
CCTGTTCATC
AGCGCGGTCC
AGGACCAGGT
GGTGCCGGAC
AACACCCTGG
CCTGGGTGTG
GGTGCGCGGC
CTGGACGAGC
TGTACGCCGA
GTGGTCGGAG
GTCGTGTCCA
CGAACTTCCG
GGACGCCTCC
GGGCCGGCCA
TGACCGAGAT
CGGCGAGCAG
CCGTGGGGGC
GGGAGTTCGC
CCTGCGCGAC
CCGGCCGGCA
ACTGCGTGCA
CTTCGTGGCC
GAGGAGCAGG
ACTGACACGT
GCTACGAGAT
TTCGATTCCA
CCGCCGCCTT
CTATGAAAGG
TTGGGCTTCG
GAATCGTTTT
CCGGGACGCC
GGCTGGATGA
TCCTCCAGCG
CGGGGATCTC
ATGCTGGAGT
TCTTCGCCCA
CCCCAACTTG
TTTATTGCAG
CTTATAATGG
TTACAAATAA
AGCAATAGCA
TCACAAATTT
CACAAATAAA
GCATTTTTTT
CACTGCATTC
TAGTTGTGGT
TTGTCCAAAC
TCATCAATGT
ATCTTATCAT
GTCTGTATAC
CGTCGACCTC
TAGCTAGAGC
TTGGCGTAAT
CATGGTCATA
GCTGTTTCCT
GTGTGAAATT
GTTATCCGCT
CACAATTCCA
CACAACATAC
GAGCCGGAAG
CATAAAGTGT
AAAGCCTGGG
GTGCCTAATG
AGTGAGCTAA
CTCACATTAA
TTGCGTTGCG
CTCACTGCCC
GCTTTCCAGT
CGGGAAACCT
GTCGTGCCAG
CTGCATTAAT
GAATCGGCCA
ACGCGCGGGG
AGAGGCGGTT
TGCGTATTGG
GCGCTCTTCC
GCTTCCTCGC
TCACTGACTC
GCTGCGCTCG
GTCGTTCGGC
TGCGGCGAGC
GGTATCAGCT
CACTCAAAGG
CGGTAATACG
GTTATCCACA
GAATCAGGGG
ATAACGCAGG
AAAGAACATG
TGAGCAAAAG
GCCAGCAAAA
GGCCAGGAAC
CGTAAAAAGG
CCGCGTTGCT
GGCGTTTTTC
CATAGGCTCC
GCCCCCCTGA
CGAGCATCAC
AAAAATCGAC
GCTCAAGTCA
GAGGTGGCGA
AACCCGACAG
GACTATAAAG
ATACCAGGCG
TTTCCCCCTG
GAAGCTCCCT
CGTGCGCTCT
CCTGTTCCGA
CCCTGCCGCT
TACCGGATAC
CTGTCCGCCT
TTCTCCCTTC
GGGAAGCGTG
GCGCTTTCTC
ATAGCTCACG
CTGTAGGTAT
CTCAGTTCGG
TGTAGGTCGT
TCGCTCCAAG
CTGGGCTGTG
TGCACGAACC
CCCCGTTCAG
CCCGACCGCT
GCGCCTTATC
CGGTAACTAT
CGTCTTGAGT
CCAACCCGGT
AAGACACGAC
TTATCGCCAC
TGGCAGCAGC
CACTGGTAAC
AGGATTAGCA
GAGCGAGGTA
TGTAGGCGGT
GCTACAGAGT
TCTTGAAGTG
GTGGCCTAAC
TACGGCTACA
CTAGAAGAAC
AGTATTTGGT
ATCTGCGCTC
TGCTGAAGCC
AGTTACCTTC
GGAAAAAGAG
TTGGTAGCTC
TTGATCCGGC
AAACAAACCA
CCGCTGGTAG
CGGTGGTTTT
TTTGTTTGCA
AGCAGCAGAT
TACGCGCAGA
AAAAAAGGAT
CTCAAGAAGA
TCCTTTGATC
TTTTCTACGG
GGTCTGACGC
TCAGTGGAAC
GAAAACTCAC
GTTAAGGGAT
TTTGGTCATG
AGATTATCAA
AAAGGATCTT
CACCTAGATC
CTTTTAAATT
AAAAATGAAG
TTTTAAATCA
ATCTAAAGTA
TATATGAGTA
AACTTGGTCT
GACAGTTACC
AATGCTTAAT
CAGTGAGGCA
CCTATCTCAG
CGATCTGTCT
ATTTCGTTCA
TCCATAGTTG
CCTGACTCCC
CGTCGTGTAG
ATAACTACGA
TACGGGAGGG
CTTACCATCT
GGCCCCAGTG
CTGCAATGAT
ACCGCGAGAC
CCACGCTCAC
CGGCTCCAGA
TTTATCAGCA
ATAAACCAGC
CAGCCGGAAG
GGCCGAGCGC
AGAAGTGGTC
CTGCAACTTT
ATCCGCCTCC
ATCCAGTCTA
TTAATTGTTG
CCGGGAAGCT
AGAGTAAGTA
GTTCGCCAGT
TAATAGTTTG
CGCAACGTTG
TTGCCATTGC
TACAGGCATC
GTGGTGTCAC
GCTCGTCGTT
TGGTATGGCT
TCATTCAGCT
CCGGTTCCCA
ACGATCAAGG
CGAGTTACAT
GATCCCCCAT
GTTGTGCAAA
AAAGCGGTTA
GCTCCTTCGG
TCCTCCGATC
GTTGTCAGAA
GTAAGTTGGC
CGCAGTGTTA
TCACTCATGG
TTATGGCAGC
ACTGCATAAT
TCTCTTACTG
TCATGCCATC
CGTAAGATGC
TTTTCTGTGA
CTGGTGAGTA
CTCAACCAAG
TCATTCTGAG
AATAGTGTAT
GCGGCGACCG
AGTTGCTCTT
GCCCGGCGTC
AATACGGGAT
AATACCGCGC
CACATAGCAG
AACTTTAAAA
GTGCTCATCA
TTGGAAAACG
TTCTTCGGGG
CGAAAACTCT
CAAGGATCTT
ACCGCTGTTG
AGATCCAGTT
CGATGTAACC
CACTCGTGCA
CCCAACTGAT
CTTCAGCATC
TTTTACTTTC
ACCAGCGTTT
CTGGGTGAGC
AAAAACAGGA
AGGCAAAATG
CCGCAAAAAA
GGGAATAAGG
GCGACACGGA
AATGTTGAAT
ACTCATACTC
TTCCTTTTTC
AATATTATTG
AAGCATTTAT
CAGGGTTATT
GTCTCATGAG
CGGATACATA
TTTGAATGTA
TTTAGAAAAA
TAAACAAATA
GGGGTTCCGC
GCACATTTCC
CCGAAAAGTG
CCACCTGAC