名称: | pLV3-CAG-ACSL5(human)-Puro |
---|---|
别称: | NM_016234.4;ACS2; ACS5; FACL5 |
基因长: | 2220bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pCDH-CAG-MCS-EF1-Puro |
正向引物: | pcaggs-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCC
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGGTT
AACTTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAT
TCAAAATTTT
ATCGATAAGC
TTGGGAGTTC
CGGACATTGA
TTATTGACTA
GTTATTAATA
GTAATCAATT
ACGGGGTCAT
TAGTTCATAG
CCCATATATG
GAGTTCCGCG
TTACATAACT
TACGGTAAAT
GGCCCGCCTG
GCTGACCGCC
CAACGACCCC
CGCCCATTGA
CGTCAATAAT
GACGTATGTT
CCCATAGTAA
CGCCAATAGG
GACTTTCCAT
TGACGTCAAT
GGGTGGAGTA
TTTACGGTAA
ACTGCCCACT
TGGCAGTACA
TCAAGTGTAT
CATATGCCAA
GTACGCCCCC
TATTGACGTC
AATGACGGTA
AATGGCCCGC
CTGGCATTAT
GCCCAGTACA
TGACCTTATG
GGACTTTCCT
ACTTGGCAGT
ACATCTACGT
ATTAGTCATC
GCTATTACCA
TGGTCGAGGT
GAGCCCCACG
TTCTGCTTCA
CTCTCCCCAT
CTCCCCCCCC
TCCCCACCCC
CAATTTTGTA
TTTATTTATT
TTTTAATTAT
TTTGTGCAGC
GATGGGGGCG
GGGGGGGGGG
GGGGGCCCCC
CCCAGGCGGG
GCGGGGCGGG
GCGAGGGGCG
GGGCGGGGCG
AGGCGGAAAG
GTGCGGCGGC
AGCCAATCAG
AGCGGCGCGC
TCCGAAAGTT
TCCTTTTATG
GCGAGGCGGC
GGCGGCGGCG
GCCCTATAAA
AAGCGAAGCG
CGCGGCGGGC
GGGAGTCGTT
GCGCGCTGCC
TTCCCCCCGT
GCCCCGCTCC
GCCGCCGCCT
CGCGCCGCCC
GCCCCGGCTC
TGACTGACCG
CGTTACTCCC
ACAGGTGAGC
GGGCGGGACG
GCCCTTCTCC
TCCGGGCTGT
AATTAGCGCT
TGGTTTAATG
ACGGCTTGTT
TCTTTTCTGT
GGCTGCGTGA
AAGCCTTGAG
GGGCTCCGGG
AGGGCCCTTT
GTGCGGGGGG
AGCGGCTCGG
GGGGTGCGTG
CGTGTGTGTG
TGCGTGGGGA
GCGCCGCGTG
CGGCTCCGCG
CTGCCCGGCG
GCTGTGAGCG
CTGCGGGCGC
GGCGCGGGGC
TTTGTGCGCT
CCGCAGTGTG
CGCGAGGGGA
GCGCGGCCGG
GGGCGGTGCC
CCGCGGTGCG
GGGGGGGCTG
CGAGGGGAAC
AAAGGCTGCG
TGCGGGGTGT
GTGCGTGGGG
GGGTGAGCAG
GGGGTGTGGG
CGCGTCGGTC
GGGCTGCAAC
CCCCCCTGCA
CCCCCCTCCC
CGAGTTGCTG
AGCACGGCCC
GGCTTCGGGT
GCGGGGCTCC
GTACGGGGCG
TGGCGCGGGG
CTCGCCGTGC
CGGGCGGGGG
GTGGCGGCAG
GTGGGGGTGC
CGGGCGGGGC
GGGGCCGCCT
CGGGCCGGGG
AGGGCTCGGG
GGAAGGGGCG
CGGCGGCCCC
CGGAGCGCCG
GCGGCTGTCG
AGGCGCGGCG
AGCCGCAGCC
ATTGCCTTTT
ATGGTAATCG
TGCGAGAGGG
CGCAGGGACT
TCCTTTGTCC
CAAATCTGTG
CGGAGCCGAA
ATCTGGGAGG
CGCCGCCGCA
CCCCCTCTAG
CGGGCGCGGG
GCGAAGCGGT
GCGGCGCCGG
CAGGAAGGAA
ATGGGCGGGG
AGGGCCTTCG
TGCGTCGCCG
CGCCGCCGTC
CCCTTCTCCC
TCTCCAGCCT
CGGGGCTGTC
CGCGGGGGGA
CGGCTGCCTT
CGGGGGGGAC
GGGGCAGGGC
GGGGTTCGGC
TTCTGGCGTG
TGACCGGCGG
CTCTAGAGCC
TCTGCTAACC
ATGTTCATGC
CTTCTTCTTT
TTCCTACAGC
TCCTGGGCAA
CGTGCTGGTT
ATTGTGCTGT
CTCATCATTT
TGGCAAAGAA
TTCGCCACCA
TGGACGCTCT
GAAGCCACCC
TGTCTCTGGA
GGAACCACGA
GCGAGGGAAG
AAGGACAGGG
ACTCGTGTGG
CAGGAAGAAC
TCAGAGCCGG
GAAGCCCCCA
TTCACTAGAA
GCACTGAGAG
ATGCGGCCCC
CTCGCAGGGT
CTGAATTTCC
TGCTGCTGTT
CACAAAGATG
CTTTTTATCT
TTAACTTTTT
GTTTTCCCCA
CTTCCGACCC
CGGCGTTGAT
CTGCATCCTG
ACATTTGGAG
CTGCCATCTT
CTTGTGGCTG
ATCACCAGAC
CTCAACCCGT
CTTACCTCTT
CTTGACCTGA
ACAATCAGTC
TGTGGGAATT
GAGGGAGGAG
CACGGAAGGG
GGTTTCCCAG
AAGAACAATG
ACCTAACAAG
TTGCTGCTTC
TCAGATGCCA
AGACTATGTA
TGAGGTTTTC
CAAAGAGGAC
TCGCTGTGTC
TGACAATGGG
CCCTGCTTGG
GATATAGAAA
ACCAAACCAG
CCCTACAGAT
GGCTATCTTA
CAAACAGGTG
TCTGATAGAG
CAGAGTACCT
GGGTTCCTGT
CTCTTGCATA
AAGGTTATAA
ATCATCACCA
GACCAGTTTG
TCGGCATCTT
TGCTCAGAAT
AGGCCAGAGT
GGATCATCTC
CGAATTGGCT
TGTTACACGT
ACTCTATGGT
AGCTGTACCT
CTGTATGACA
CCTTGGGACC
AGAAGCCATC
GTACATATTG
TCAACAAGGC
TGATATCGCC
ATGGTGATCT
GTGACACACC
CCAAAAGGCA
TTGGTGCTGA
TAGGGAATGT
AGAGAAAGGC
TTCACCCCGA
GCCTGAAGGT
GATCATCCTT
ATGGACCCCT
TTGATGATGA
CCTGAAGCAA
AGAGGGGAGA
AGAGTGGAAT
TGAGATCTTA
TCCCTATATG
ATGCTGAGAA
CCTAGGCAAA
GAGCACTTCA
GAAAACCTGT
GCCTCCTAGC
CCAGAAGACC
TGAGCGTCAT
CTGCTTCACC
AGTGGGACCA
CAGGTGACCC
CAAAGGAGCC
ATGATAACCC
ATCAAAATAT
TGTTTCAAAT
GCTGCTGCCT
TTCTCAAATG
TGTGGAGCAT
GCTTATGAGC
CCACTCCTGA
TGATGTGGCC
ATATCCTACC
TCCCTCTGGC
TCATATGTTT
GAGAGGATTG
TACAGGCTGT
TGTGTACAGC
TGTGGAGCCA
GAGTTGGATT
CTTCCAAGGG
GATATTCGGT
TGCTGGCTGA
CGACATGAAG
ACTTTGAAGC
CCACATTGTT
TCCCGCGGTG
CCTCGACTCC
TTAACAGGAT
CTACGATAAG
GTACAAAATG
AGGCCAAGAC
ACCCTTGAAG
AAGTTCTTGT
TGAAGCTGGC
TGTTTCCAGT
AAATTCAAAG
AGCTTCAAAA
GGGTATCATC
AGGCATGATA
GTTTCTGGGA
CAAGCTCATC
TTTGCAAAGA
TCCAGGACAG
CCTGGGCGGA
AGGGTTCGTG
TAATTGTCAC
TGGAGCTGCC
CCCATGTCCA
CTTCAGTCAT
GACATTCTTC
CGGGCAGCAA
TGGGATGTCA
GGTGTATGAA
GCTTATGGTC
AAACAGAATG
CACAGGTGGC
TGTACATTTA
CATTACCTGG
GGACTGGACA
TCAGGTCACG
TTGGGGTGCC
CCTGGCTTGC
AATTACGTGA
AGCTGGAAGA
TGTGGCTGAC
ATGAACTACT
TTACAGTGAA
TAATGAAGGA
GAGGTCTGCA
TCAAGGGTAC
AAACGTGTTC
AAAGGATACC
TGAAGGACCC
TGAGAAGACA
CAGGAAGCCC
TGGACAGTGA
TGGCTGGCTT
CACACAGGAG
ACATTGGTCG
CTGGCTCCCG
AATGGAACTC
TGAAGATCAT
CGACCGTAAA
AAGAACATTT
TCAAGCTGGC
CCAAGGAGAA
TACATTGCAC
CAGAGAAGAT
AGAAAATATC
TACAACAGGA
GTCAACCAGT
GTTACAAATT
TTTGTACACG
GGGAGAGCTT
ACGGTCATCC
TTAGTAGGAG
TGGTGGTTCC
TGACACAGAT
GTACTTCCCT
CATTTGCAGC
CAAGCTTGGG
GTGAAGGGCT
CCTTTGAGGA
ACTGTGCCAA
AACCAAGTTG
TAAGGGAAGC
CATTTTAGAA
GACTTGCAGA
AAATTGGGAA
AGAAAGTGGC
CTTAAAACTT
TTGAACAGGT
CAAAGCCATT
TTTCTTCATC
CAGAGCCATT
TTCCATTGAA
AATGGGCTCT
TGACACCAAC
ATTGAAAGCA
AAGCGAGGAG
AGCTTTCCAA
ATACTTTCGG
ACCCAAATTG
ACAGCCTGTA
TGAGCACATC
CAGGATTAGG
CGGCCGCAAG
GATCTGCGAT
CGCTCCGGTG
CCCGTCAGTG
GGCAGAGCGC
ACATCGCCCA
CAGTCCCCGA
GAAGTTGGGG
GGAGGGGTCG
GCAATTGAAC
GGGTGCCTAG
AGAAGGTGGC
GCGGGGTAAA
CTGGGAAAGT
GATGTCGTGT
ACTGGCTCCG
CCTTTTTCCC
GAGGGTGGGG
GAGAACCGTA
TATAAGTGCA
GTAGTCGCCG
TGAACGTTCT
TTTTCGCAAC
GGGTTTGCCG
CCAGAACACA
GCTGAAGCTT
CGAGGGGCTC
GCATCTCTCC
TTCACGCGCC
CGCCGCCCTA
CCTGAGGCCG
CCATCCACGC
CGGTTGAGTC
GCGTTCTGCC
GCCTCCCGCC
TGTGGTGCCT
CCTGAACTGC
GTCCGCCGTC
TAGGTAAGTT
TAAAGCTCAG
GTCGAGACCG
GGCCTTTGTC
CGGCGCTCCC
TTGGAGCCTA
CCTAGACTCA
GCCGGCTCTC
CACGCTTTGC
CTGACCCTGC
TTGCTCAACT
CTACGTCTTT
GTTTCGTTTT
CTGTTCTGCG
CCGTTACAGA
TCCAAGCTGT
GACCGGCGCC
TACGCTAGAT
GACCGAGTAC
AAGCCCACGG
TGCGCCTCGC
CACCCGCGAC
GACGTCCCCA
GGGCCGTACG
CACCCTCGCC
GCCGCGTTCG
CCGACTACCC
CGCCACGCGC
CACACCGTCG
ATCCGGACCG
CCACATCGAG
CGGGTCACCG
AGCTGCAAGA
ACTCTTCCTC
ACGCGCGTCG
GGCTCGACAT
CGGCAAGGTG
TGGGTCGCGG
ACGACGGCGC
CGCGGTGGCG
GTCTGGACCA
CGCCGGAGAG
CGTCGAAGCG
GGGGCGGTGT
TCGCCGAGAT
CGGCCCGCGC
ATGGCCGAGT
TGAGCGGTTC
CCGGCTGGCC
GCGCAGCAAC
AGATGGAAGG
CCTCCTGGCG
CCGCACCGGC
CCAAGGAGCC
CGCGTGGTTC
CTGGCCACCG
TCGGCGTCTC
GCCCGACCAC
CAGGGCAAGG
GTCTGGGCAG
CGCCGTCGTG
CTCCCCGGAG
TGGAGGCGGC
CGAGCGCGCC
GGGGTGCCCG
CCTTCCTGGA
GACCTCCGCG
CCCCGCAACC
TCCCCTTCTA
CGAGCGGCTC
GGCTTCACCG
TCACCGCCGA
CGTCGAGGTG
CCCGAAGGAC
CGCGCACCTG
GTGCATGACC
CGCAAGCCCG
GTGCCTGAAA
TCAACCTCTG
GATTACAAAA
TTTGTGAAAG
ATTGACTGGT
ATTCTTAACT
ATGTTGCTCC
TTTTACGCTA
TGTGGATACG
CTGCTTTAAT
GCCTTTGTAT
CATGCTATTG
CTTCCCGTAT
GGCTTTCATT
TTCTCCTCCT
TGTATAAATC
CTGGTTGCTG
TCTCTTTATG
AGGAGTTGTG
GCCCGTTGTC
AGGCAACGTG
GCGTGGTGTG
CACTGTGTTT
GCTGACGCAA
CCCCCACTGG
TTGGGGCATT
GCCACCACCT
GTCAGCTCCT
TTCCGGGACT
TTCGCTTTCC
CCCTCCCTAT
TGCCACGGCG
GAACTCATCG
CCGCCTGCCT
TGCCCGCTGC
TGGACAGGGG
CTCGGCTGTT
GGGCACTGAC
AATTCCGTGG
TGTTGTCGGG
GAAATCATCG
TCCTTTCCTT
GGCTGCTCGC
CTGTGTTGCC
ACCTGGATTC
TGCGCGGGAC
GTCCTTCTGC
TACGTCCCTT
CGGCCCTCAA
TCCAGCGGAC
CTTCCTTCCC
GCGGCCTGCT
GCCGGCTCTG
CGGCCTCTTC
CGCGTCTTCG
CCTTCGCCCT
CAGACGAGTC
GGATCTCCCT
TTGGGCCGCC
TCCCCGCCTG
GTACCTTTAA
GACCAATGAC
TTACAAGGCA
GCTGTAGATC
TTAGCCACTT
TTTAAAAGAA
AAGGGGGGAC
TGGAAGGGCT
AATTCACTCC
CAACGAAAAT
AAGATCTGCT
TTTTGCTTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTAGTA
GTTCATGTCA
TCTTATTATT
CAGTATTTAT
AACTTGCAAA
GAAATGAATA
TCAGAGAGTG
AGAGGAACTT
GTTTATTGCA
GCTTATAATG
GTTACAAATA
AAGCAATAGC
ATCACAAATT
TCACAAATAA
AGCATTTTTT
TCACTGCATT
CTAGTTGTGG
TTTGTCCAAA
CTCATCAATG
TATCTTATCA
TGTCTGGCTC
TAGCTATCCC
GCCCCTAACT
CCGCCCAGTT
CCGCCCATTC
TCCGCCCCAT
GGCTGACTAA
TTTTTTTTAT
TTATGCAGAG
GCCGAGGCCG
CCTCGGCCTC
TGAGCTATTC
CAGAAGTAGT
GAGGAGGCTT
TTTTGGAGGC
CTAGACTTTT
GCAGAGACGG
CCCAAATTCG
TAATCATGGT
CATAGCTGTT
TCCTGTGTGA
AATTGTTATC
CGCTCACAAT
TCCACACAAC
ATACGAGCCG
GAAGCATAAA
GTGTAAAGCC
TGGGGTGCCT
AATGAGTGAG
CTAACTCACA
TTAATTGCGT
TGCGCTCACT
GCCCGCTTTC
CAGTCGGGAA
ACCTGTCGTG
CCAGCTGCAT
TAATGAATCG
GCCAACGCGC
GGGGAGAGGC
GGTTTGCGTA
TTGGGCGCTC
TTCCGCTTCC
TCGCTCACTG
ACTCGCTGCG
CTCGGTCGTT
CGGCTGCGGC
GAGCGGTATC
AGCTCACTCA
AAGGCGGTAA
TACGGTTATC
CACAGAATCA
GGGGATAACG
CAGGAAAGAA
CATGTGAGCA
AAAGGCCAGC
AAAAGGCCAG
GAACCGTAAA
AAGGCCGCGT
TGCTGGCGTT
TTTCCATAGG
CTCCGCCCCC
CTGACGAGCA
TCACAAAAAT
CGACGCTCAA
GTCAGAGGTG
GCGAAACCCG
ACAGGACTAT
AAAGATACCA
GGCGTTTCCC
CCTGGAAGCT
CCCTCGTGCG
CTCTCCTGTT
CCGACCCTGC
CGCTTACCGG
ATACCTGTCC
GCCTTTCTCC
CTTCGGGAAG
CGTGGCGCTT
TCTCATAGCT
CACGCTGTAG
GTATCTCAGT
TCGGTGTAGG
TCGTTCGCTC
CAAGCTGGGC
TGTGTGCACG
AACCCCCCGT
TCAGCCCGAC
CGCTGCGCCT
TATCCGGTAA
CTATCGTCTT
GAGTCCAACC
CGGTAAGACA
CGACTTATCG
CCACTGGCAG
CAGCCACTGG
TAACAGGATT
AGCAGAGCGA
GGTATGTAGG
CGGTGCTACA
GAGTTCTTGA
AGTGGTGGCC
TAACTACGGC
TACACTAGAA
GGACAGTATT
TGGTATCTGC
GCTCTGCTGA
AGCCAGTTAC
CTTCGGAAAA
AGAGTTGGTA
GCTCTTGATC
CGGCAAACAA
ACCACCGCTG
GTAGCGGTGG
TTTTTTTGTT
TGCAAGCAGC
AGATTACGCG
CAGAAAAAAA
GGATCTCAAG
AAGATCCTTT
GATCTTTTCT
ACGGGGTCTG
ACGCTCAGTG
GAACGAAAAC
TCACGTTAAG
GGATTTTGGT
CATGAGATTA
TCAAAAAGGA
TCTTCACCTA
GATCCTTTTA
AATTAAAAAT
GAAGTTTTAA
ATCAATCTAA
AGTATATATG
AGTAAACTTG
GTCTGACAGT
TACCAATGCT
TAATCAGTGA
GGCACCTATC
TCAGCGATCT
GTCTATTTCG
TTCATCCATA
GTTGCCTGAC
TCCCCGTCGT
GTAGATAACT
ACGATACGGG
AGGGCTTACC
ATCTGGCCCC
AGTGCTGCAA
TGATACCGCG
AGACCCACGC
TCACCGGCTC
CAGATTTATC
AGCAATAAAC
CAGCCAGCCG
GAAGGGCCGA
GCGCAGAAGT
GGTCCTGCAA
CTTTATCCGC
CTCCATCCAG
TCTATTAATT
GTTGCCGGGA
AGCTAGAGTA
AGTAGTTCGC
CAGTTAATAG
TTTGCGCAAC
GTTGTTGCCA
TTGCTACAGG
CATCGTGGTG
TCACGCTCGT
CGTTTGGTAT
GGCTTCATTC
AGCTCCGGTT
CCCAACGATC
AAGGCGAGTT
ACATGATCCC
CCATGTTGTG
CAAAAAAGCG
GTTAGCTCCT
TCGGTCCTCC
GATCGTTGTC
AGAAGTAAGT
TGGCCGCAGT
GTTATCACTC
ATGGTTATGG
CAGCACTGCA
TAATTCTCTT
ACTGTCATGC
CATCCGTAAG
ATGCTTTTCT
GTGACTGGTG
AGTACTCAAC
CAAGTCATTC
TGAGAATAGT
GTATGCGGCG
ACCGAGTTGC
TCTTGCCCGG
CGTCAATACG
GGATAATACC
GCGCCACATA
GCAGAACTTT
AAAAGTGCTC
ATCATTGGAA
AACGTTCTTC
GGGGCGAAAA
CTCTCAAGGA
TCTTACCGCT
GTTGAGATCC
AGTTCGATGT
AACCCACTCG
TGCACCCAAC
TGATCTTCAG
CATCTTTTAC
TTTCACCAGC
GTTTCTGGGT
GAGCAAAAAC
AGGAAGGCAA
AATGCCGCAA
AAAAGGGAAT
AAGGGCGACA
CGGAAATGTT
GAATACTCAT
ACTCTTCCTT
TTTCAATATT
ATTGAAGCAT
TTATCAGGGT
TATTGTCTCA
TGAGCGGATA
CATATTTGAA
TGTATTTAGA
AAAATAAACA
AATAGGGGTT
CCGCGCACAT
TTCCCCGAAA
AGTGCCACCT
GACGTCTAAG
AAACCATTAT
TATCATGACA
TTAACCTATA
AAAATAGGCG
TATCACGAGG
CCCTTTCGTC
TCGCGCGTTT
CGGTGATGAC
GGTGAAAACC
TCTGACACAT
GCAGCTCCCG
GAGACGGTCA
CAGCTTGTCT
GTAAGCGGAT
GCCGGGAGCA
GACAAGCCCG
TCAGGGCGCG
TCAGCGGGTG
TTGGCGGGTG
TCGGGGCTGG
CTTAACTATG
CGGCATCAGA
GCAGATTGTA
CTGAGAGTGC
ACCATATGCG
GTGTGAAATA
CCGCACAGAT
GCGTAAGGAG
AAAATACCGC
ATCAGGCGCC
ATTCGCCATT
CAGGCTGCGC
AACTGTTGGG
AAGGGCGATC
GGTGCGGGCC
TCTTCGCTAT
TACGCCAGCT
GGCGAAAGGG
GGATGTGCTG
CAAGGCGATT
AAGTTGGGTA
ACGCCAGGGT
TTTCCCAGTC
ACGACGTTGT
AAAACGACGG
CCAGTGCCAA
GCTG