名称: | pSin-EF2-BHLHE40(human)-FLAG-Puro |
---|---|
别称: | NM_003670.3;BHLHB2; Clast5; DEC1; HLHB2; SHARP-2;SHARP2; STRA13; Stra14 |
基因长: | 1236bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | EF-1ɑ |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pSIN-EF2-Puro |
正向引物: | PEF-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CGGACCGCCA
CTGCCAATTA
CCTGTGGTTT
CATTTACTCT
AAACCTGTGA
TTCCTCTGAA
TTATTTTCAT
TTTAAAGAAA
TTGTATTTGT
TAAATATGTA
CTACAAACTT
AGTAGTTGGA
AGGGCTAATT
CACTCCCAAA
GAAGACAAGA
TATCCTTGAT
CTGTGGATCT
ACCACACACA
AGGCTACTTC
CCTGATTAGC
AGAACTACAC
ACCAGGGCCA
GGGGTCAGAT
ATCCACTGAC
CTTTGGATGG
TGCTACAAGC
TAGTACCAGT
TGAGCCAGAT
AAGGTAGAAG
AGGCCAATAA
AGGAGAGAAC
ACCAGCTTGT
TACACCCTGT
GAGCCTGCAT
GGGATGGATG
ACCCGGAGAG
AGAAGTGTTA
GAGTGGAGGT
TTGACAGCCG
CCTAGCATTT
CATCACGTGG
CCCGAGAGCT
GCATCCGGAG
TACTTCAAGA
ACTGCTGATA
TCGAGCTTGC
TACAAGGGAC
TTTCCGCTGG
GGACTTTCCA
GGGAGGCGTG
GCCTGGGCGG
GACTGGGGAG
TGGCGAGCCC
TCAGATCCTG
CATATAAGCA
GCTGCTTTTT
GCCTGTACTG
GGTCTCTCTG
GTTAGACCAG
ATCTGAGCCT
GGGAGCTCTC
TGGCTAACTA
GGGAACCCAC
TGCTTAAGCC
TCAATAAAGC
TTGCCTTGAG
TGCTTCAAGT
AGTGTGTGCC
CGTCTGTTGT
GTGACTCTGG
TAACTAGAGA
TCCCTCAGAC
CCTTTTAGTC
AGTGTGGAAA
ATCTCTAGCA
GTGGCGCCCG
AACAGGGACT
TGAAAGCGAA
AGGGAAACCA
GAGGAGCTCT
CTCGACGCAG
GACTCGGCTT
GCTGAAGCGC
GCACGGCAAG
AGGCGAGGGG
CGGCGACTGG
TGAGTACGCC
AAAAATTTTG
ACTAGCGGAG
GCTAGAAGGA
GAGAGATGGG
TGCGAGAGCG
TCAGTATTAA
GCGGGGGAGA
ATTAGATCGC
GATGGGAAAA
AATTCGGTTA
AGGCCAGGGG
GAAAGAAAAA
ATATAAATTA
AAACATATAG
TATGGGCAAG
CAGGGAGCTA
GAACGATTCG
CAGTTAATCC
TGGCCTGTTA
GAAACATCAG
AAGGCTGTAG
ACAAATACTG
GGACAGCTAC
AACCATCCCT
TCAGACAGGA
TCAGAAGAAC
TTAGATCATT
ATATAATACA
GTAGCAACCC
TCTATTGTGT
GCATCAAAGG
ATAGAGATAA
AAGACACCAA
GGAAGCTTTA
GACAAGATAG
AGGAAGAGCA
AAACAAAAGT
AAGACCACCG
CACAGCAAGC
GGCCGCTGAT
CTTCAGACCT
GGAGGAGGAG
ATATGAGGGA
CAATTGGAGA
AGTGAATTAT
ATAAATATAA
AGTAGTAAAA
ATTGAACCAT
TAGGAGTAGC
ACCCACCAAG
GCAAAGAGAA
GAGTGGTGCA
GAGAGAAAAA
AGAGCAGTGG
GAATAGGAGC
TTTGTTCCTT
GGGTTCTTGG
GAGCAGCAGG
AAGCACTATG
GGCGCAGCGT
CAATGACGCT
GACGGTACAG
GCCAGACAAT
TATTGTCTGG
TATAGTGCAG
CAGCAGAACA
ATTTGCTGAG
GGCTATTGAG
GCGCAACAGC
ATCTGTTGCA
ACTCACAGTC
TGGGGCATCA
AGCAGCTCCA
GGCAAGAATC
CTGGCTGTGG
AAAGATACCT
AAAGGATCAA
CAGCTCCTGG
GGATTTGGGG
TTGCTCTGGA
AAACTCATTT
GCACCACTGC
TGTGCCTTGG
AATGCTAGTT
GGAGTAATAA
ATCTCTGGAA
CAGATTTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGCCA
CAAATGGCAG
TATTCATCCA
CAATTTTAAA
AGAAAGGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TCGGGTTTAT
TACAGGGACA
GCAGAGATCC
ACTTTGGATC
GATAAGCTTT
GCAAAGATGG
ATAAAGTTTT
AAACAGAGAG
GAATCTTTGC
AGCTAATGGA
CCTTCTAGGT
CTTGAAAGGA
GTGGGAATTG
GCTCCGGTGC
CCGTCAGTGG
GCAGAGCGCA
CATCGCCCAC
AGTCCCCGAG
AAGTTGGGGG
GAGGGGTCGG
CAATTGAACC
GGTGCCTAGA
GAAGGTGGCG
CGGGGTAAAC
TGGGAAAGTG
ATGTCGTGTA
CTGGCTCCGC
CTTTTTCCCG
AGGGTGGGGG
AGAACCGTAT
ATAAGTGCAG
TAGTCGCCGT
GAACGTTCTT
TTTCGCAACG
GGTTTGCCGC
CAGAACACAG
GTAAGTGCCG
TGTGTGGTTC
CCGCGGGCCT
GGCCTCTTTA
CGGGTTATGG
CCCTTGCGTG
CCTTGAATTA
CTTCCACCTG
GCTGCAGTAC
GTGATTCTTG
ATCCCGAGCT
TCGGGTTGGA
AGTGGGTGGG
AGAGTTCGAG
GCCTTGCGCT
TAAGGAGCCC
CTTCGCCTCG
TGCTTGAGTT
GAGGCCTGGC
CTGGGCGCTG
GGGCCGCCGC
GTGCGAATCT
GGTGGCACCT
TCGCGCCTGT
CTCGCTGCTT
TCGATAAGTC
TCTAGCCATT
TAAAATTTTT
GATGACCTGC
TGCGACGCTT
TTTTTCTGGC
AAGATAGTCT
TGTAAATGCG
GGCCAAGATC
TGCACACTGG
TATTTCGGTT
TTTGGGGCCG
CGGGCGGCGA
CGGGGCCCGT
GCGTCCCAGC
GCACATGTTC
GGCGAGGCGG
GGCCTGCGAG
CGCGGCCACC
GAGAATCGGA
CGGGGGTAGT
CTCAAGCTGG
CCGGCCTGCT
CTGGTGCCTG
GCCTCGCGCC
GCCGTGTATC
GCCCCGCCCT
GGGCGGCAAG
GCTGGCCCGG
TCGGCACCAG
TTGCGTGAGC
GGAAAGATGG
CCGCTTCCCG
GCCCTGCTGC
AGGGAGCTCA
AAATGGAGGA
CGCGGCGCTC
GGGAGAGCGG
GCGGGTGAGT
CACCCACACA
AAGGAAAAGG
GCCTTTCCGT
CCTCAGCCGT
CGCTTCATGT
GACTCCACGG
AGTACCGGGC
GCCGTCCAGG
CACCTCGATT
AGTTCTCGAG
CTTTTGGAGT
ACGTCGTCTT
TAGGTTGGGG
GGAGGGGTTT
TATGCGATGG
AGTTTCCCCA
CACTGAGTGG
GTGGAGACTG
AAGTTAGGCC
AGCTTGGCAC
TTGATGTAAT
TCTCCTTGGA
ATTTGCCCTT
TTTGAGTTTG
GATCTTGGTT
CATTCTCAAG
CCTCAGACAG
TGGTTCAAAG
TTTTTTTCTT
CCATTTCAGG
TGTCGTGAGG
AATTGGGATC
CGCCACCATG
GAGCGGATCC
CCAGCGCGCA
ACCACCCCCC
GCCTGCCTGC
CCAAAGCACC
GGGACTGGAG
CACGGAGACC
TACCAGGGAT
GTACCCTGCC
CACATGTACC
AAGTGTACAA
GTCAAGACGG
GGAATAAAGC
GGAGCGAGGA
CAGCAAGGAG
ACCTACAAAT
TGCCGCACCG
GCTCATCGAG
AAAAAGAGAC
GTGACCGGAT
TAACGAGTGC
ATCGCCCAGC
TGAAGGATCT
CCTACCCGAA
CATCTCAAAC
TTACAACTTT
GGGTCACTTG
GAAAAAGCAG
TGGTTCTTGA
ACTTACCTTG
AAGCATGTGA
AAGCACTAAC
AAACCTAATT
GATCAGCAGC
AGCAGAAAAT
CATTGCCCTG
CAGAGTGGTT
TACAAGCTGG
TGAGCTGTCA
GGGAGAAATG
TCGAAACAGG
TCAAGAGATG
TTCTGCTCAG
GTTTCCAGAC
ATGTGCCCGG
GAGGTGCTTC
AGTATCTGGC
CAAGCACGAG
AACACTCGGG
ACCTGAAGTC
TTCGCAGCTT
GTCACCCACC
TCCACCGGGT
GGTCTCGGAG
CTGCTGCAGG
GTGGTACCTC
CAGGAAGCCA
TCAGACCCAG
CTCCCAAAGT
GATGGACTTC
AAGGAAAAAC
CCAGCTCTCC
GGCCAAAGGT
TCGGAAGGTC
CTGGGAAAAA
CTGCGTGCCA
GTCATCCAGC
GGACTTTCGC
TCACTCGAGT
GGGGAGCAGA
GCGGCAGCGA
CACGGACACA
GACAGTGGCT
ATGGAGGAGA
ATCGGAGAAG
GGCGACTTGC
GCAGTGAGCA
GCCGTGCTTC
AAAAGTGACC
ACGGACGCAG
GTTCACGATG
GGAGAAAGGA
TCGGCGCAAT
TAAGCAAGAG
TCCGAAGAAC
CCCCCACAAA
AAAGAACCGG
ATGCAGCTTT
CGGATGATGA
AGGCCATTTC
ACTAGCAGTG
ACCTGATCAG
CTCCCCGTTC
CTGGGCCCAC
ACCCACACCA
GCCTCCTTTC
TGCCTGCCCT
TCTACCTGAT
CCCACCTTCA
GCGACTGCCT
ACCTGCCCAT
GCTGGAGAAG
TGCTGGTATC
CCACCTCAGT
GCCAGTGCTA
TACCCAGGCC
TCAACGCCTC
TGCCGCAGCC
CTCTCTAGCT
TCATGAACCC
AGACAAGATC
TCGGCTCCCT
TGCTCATGCC
CCAGAGACTC
CCTTCTCCCT
TGCCAGCTCA
TCCGTCCGTC
GACTCTTCTG
TCTTGCTCCA
AGCTCTGAAG
CCAATCCCCC
CTTTAAACTT
AGAAACCAAA
GACGATTACA
AGGACGACGA
TGACAAGTAA
GAATTCTTCG
AACATATGAA
GCTTGCTAGC
ACTAGTGATC
CGCATGCATC
TAGGGCGGCC
AATTCCGCCC
CTCTCCCCCC
CCCCCCTCTC
CCTCCCCCCC
CCCTAACGTT
ACTGGCCGAA
GCCGCTTGGA
ATAAGGCCGG
TGTGCGTTTG
TCTATATGTT
ATTTTCCACC
ATATTGCCGT
CTTTTGGCAA
TGTGAGGGCC
CGGAAACCTG
GCCCTGTCTT
CTTGACGAGC
ATTCCTAGGG
GTCTTTCCCC
TCTCGCCAAA
GGAATGCAAG
GTCTGTTGAA
TGTCGTGAAG
GAAGCAGTTC
CTCTGGAAGC
TTCTTGAAGA
CAAACAACGT
CTGTAGCGAC
CCTTTGCAGG
CAGCGGAACC
CCCCACCTGG
CGACAGGTGC
CTCTGCGGCC
AAAAGCCACG
TGTATAAGAT
ACACCTGCAA
AGGCGGCACA
ACCCCAGTGC
CACGTTGTGA
GTTGGATAGT
TGTGGAAAGA
GTCAAATGGC
TCTCCTCAAG
CGTATTCAAC
AAGGGGCTGA
AGGATGCCCA
GAAGGTACCC
CATTGTATGG
GATCTGATCT
GGGGCCTCGG
TGCACATGCT
TTACATGTGT
TTAGTCGAGG
TTAAAAAAAC
GTCTAGGCCC
CCCGAACCAC
GGGGACGTGG
TTTTCCTTTG
AAAAACACGA
TGATAAGCTT
GCCACAACCC
ACAAGGAGAC
GACCTTCCAT
GACCGAGTAC
AAGCCCACGG
TGCGCCTCGC
CACCCGCGAC
GACGTCCCCC
GGGCCGTACG
CACCCTCGCC
GCCGCGTTCG
CCGACTACCC
CGCCACGCGC
CACACCGTCG
ACCCGGACCG
CCACATCGAG
CGGGTCACCG
AGCTGCAAGA
ACTCTTCCTC
ACGCGCGTCG
GGCTCGACAT
CGGCAAGGTG
TGGGTCGCGG
ACGACGGCGC
CGCGGTGGCG
GTCTGGACCA
CGCCGGAGAG
CGTCGAAGCG
GGGGCGGTGT
TCGCCGAGAT
CGGCCCGCGC
ATGGCCGAGT
TGAGCGGTTC
CCGGCTGGCC
GCGCAGCAAC
AGATGGAAGG
CCTCCTGGCG
CCGCACCGGC
CCAAGGAGCC
CGCGTGGTTC
CTGGCCACCG
TCGGCGTCTC
GCCCGACCAC
CAGGGCAAGG
GTCTGGGCAG
CGCCGTCGTG
CTCCCCGGAG
TGGAGGCGGC
CGAGCGCGCC
GGGGTGCCCG
CCTTCCTGGA
GACCTCCGCG
CCCCGCAACC
TCCCCTTCTA
CGAGCGGCTC
GGCTTCACCG
TCACCGCCGA
CGTCGAGGTG
CCCGAAGGAC
CGCGCACCTG
GTGCATGACC
CGCAAGCCCG
GTGCCTGATA
ATAGGCGGCC
GCTCGAGACC
TAGAAAAACA
TGGAGCAATC
ACAAGTAGCA
ATACAGCAGC
TACCAATGCT
GATTGTGCCT
GGCTAGAAGC
ACAAGAGGAG
GAGGAGGTGG
GTTTTCCAGT
CACACCTCAG
GTACCTTTAA
GACCAATGAC
TTACAAGGCA
GCTGTAGATC
TTAGCCACTT
TTTAAAAGAA
AAGGGGGGAC
TGGAAGGGCT
AATTCACTCC
CAACGAAGAC
AAGATCTGCT
TTTTGCTTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTAGTA
GTTCATGTCA
TCTTATTATT
CAGTATTTAT
AACTTGCAAA
GAAATGAATA
TCAGAGAGTG
AGAGGCCTTG
ACATTATAAT
AGATTTAGCA
GGAATTGAAC
TAGGAGTGGA
GCACACAGGC
AAAGTTCTAG
AGCTCGCTGA
TCAGCCTCGA
CTGTGCCTTC
TAGTTGCCAG
CCATCTGTTG
TTTGCCCCTC
CCCCGTGCCT
TCCTTGACCC
TGGAAGGTGC
CACTCCCACT
GTCCTTTCCT
AATAAAATGA
GGAAATTGCA
TCGCATTGTC
TGAGTAGGTG
TCATTCTATT
CTGGGGGGTG
GGGGTGGGGC
AGGACAGCAA
GGGGGGAGGA
TTGGGAAGAC
AATAGCAGGC
ATGCTGGGGA
TGCGGTGGGC
TCTATGGCTT
CTGAGGCGGA
AAGAACCAGC
TGGGGGCGCG
CCCCTCGAGG
CCGCCATGGT
CATAGCTGTT
TGACGTCAGG
TGGCACTTTT
CGGGGAAATG
TGCGCGGAAC
CCCTATTTGT
TTATTTTTCT
AAATACATTC
AAATATGTAT
CCGCTCACGA
GACAATAACC
CTGATAAATG
CTTCAATAAT
ATTGAAAAAG
GAAGAGTATG
AGTATTCAAC
ATTTCCGTGT
CGCCCTTATT
CCCTTTTTTG
CGGCATTTTG
CCTTCCTGTT
TTTGCTCACC
CAGAAACGCT
GGTGAAAGTA
AAAGATGCTG
AAGATCAGTT
GGGTGCACGA
GTGGGTTACA
TCGAACTGGA
TCTCAACAGC
GGTAAGATCC
TTGAGAGTTT
TCGCCCCGAA
GAACGTTTTC
CAATGATGAG
CACTTTTAAA
GTTCTGCTAT
GTGGCGCGGT
ATTATCCCGT
ATTGACGCCG
GGCAAGAGCA
ACTCGGTCGC
CGCATACACT
ATTCTCAGAA
TGACTTGGTT
GAGTACTCAC
CAGTCACAGA
AAAGCATCTT
ACGGATGGCA
TGACAGTAAG
AGAATTATGC
AGTGCTGCCA
TAACCATGAG
TGATAACACT
GCGGCCAACT
TACTTCTGAC
AACGATCGGA
GGACCGAAGG
AGCTAACCGC
TTTTTTGCAC
AACATGGGGG
ATCATGTAAC
TCGCCTTGAT
CGTTGGGAAC
CGGAGCTGAA
TGAAGCCATA
CCAAACGACG
AGCGTGACAC
CACGATGCCT
GTAGCAATGG
CAACAACGTT
GCGCAAACTA
TTAACTGGCG
AACTACTTAC
TCTAGCTTCC
CGGCAACAAT
TAATAGACTG
GATGGAGGCG
GATAAAGTTG
CAGGACCACT
TCTGCGCTCG
GCCCTTCCGG
CTGGCTGGTT
TATTGCTGAT
AAATCTGGAG
CCGGTGAGCG
TGGGTCTCGC
GGTATCATTG
CAGCACTGGG
GCCAGATGGT
AAGCCCTCCC
GTATCGTAGT
TATCTACACG
ACGGGGAGTC
AGGCAACTAT
GGATGAACGA
AATAGACAGA
TCGCTGAGAT
AGGTGCCTCA
CTGATTAAGC
ATTGGTAACT
GTCAGACCAA
GTTTACTCAT
ATATACTTTA
GATTGATTTA
AAACTTCATT
TTTAATTTAA
AAGGATCTAG
GTGAAGATCC
TTTTTGATAA
TCTCATGACC
AAAATCCCTT
AACGTGAGTT
TTCGTTCCAC
TGAGCGTCAG
ACCCCGTAGA
AAAGATCAAA
GGATCTTCTT
GAGATCCTTT
TTTTCTGCGC
GTAATCTGCT
GCTTGCAAAC
AAAAAAACCA
CCGCTACCAG
CGGTGGTTTG
TTTGCCGGAT
CAAGAGCTAC
CAACTCTTTT
TCCGAAGGTA
ACTGGCTTCA
GCAGAGCGCA
GATACCAAAT
ACTGTTCTTC
TAGTGTAGCC
GTAGTTAGGC
CACCACTTCA
AGAACTCTGT
AGCACCGCCT
ACATACCTCG
CTCTGCTAAT
CCTGTTACCA
GTGGCTGCTG
CCAGTGGCGA
TAAGTCGTGT
CTTACCGGGT
TGGACTCAAG
ACGATAGTTA
CCGGATAAGC
GCAGCGGTCG
GGCTGAACGG
GGGGTTCGTG
CACACAGCCC
AGCTTGGAGC
GAACGACCTA
CACCGAACTG
AGATACCTAC
AGCGTGAGCT
ATGAGAAAGC
GCCACGCTTC
CCGAAGGGAG
AAAGGCGGAC
AGGTATCCGG
TAAGCGGCAG
GGTCGGAACA
GGAGAGCGCA
CGAGGGAGCT
TCCAGGGGGA
AACGCCTGGT
ATCTTTATAG
TCCTGTCGGG
TTTCGCCACC
TCTGACTTGA
GCGTCGATTT
TTGTGATGCT
CGTCAGGGGG
GCGGAGCCTA
TGGAAAAACG
CCAGCAACGC
GGCCTTTTTA
CGGTTCTGGC
CTTTTGCA