名称: | pHAGE-TO-dCas9-3XGFP |
---|---|
别称: | Plasmid #64107 |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | CMV/TO |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pHAGE-TO-dCas9② |
正向引物: | WPRE-R(重复序列) |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGGAAGGGCT
AATTCACTCC
CAAAGAAGAC
AAGATATCCT
TGATCTGTGG
ATCTACCACA
CACAAGGCTA
CTTCCCTGAT
TAGCAGAACT
ACACACCAGG
GCCAGGGGTC
AGATATCCAC
TGACCTTTGG
ATGGTGCTAC
AAGCTAGTAC
CAGTTGAGCC
AGATAAGGTA
GAAGAGGCCA
ATAAAGGAGA
GAACACCAGC
TTGTTACACC
CTGTGAGCCT
GCATGGGATG
GATGACCCGG
AGAGAGAAGT
GTTAGAGTGG
AGGTTTGACA
GCCGCCTAGC
ATTTCATCAC
GTGGCCCGAG
AGCTGCATCC
GGAGTACTTC
AAGAACTGCT
GATATCGAGC
TTGCTACAAG
GGACTTTCCG
CTGGGGACTT
TCCAGGGAGG
CGTGGCCTGG
GCGGGACTGG
GGAGTGGCGA
GCCCTCAGAT
CCTGCATATA
AGCAGCTGCT
TTTTGCCTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACTTGAAAG
CGAAAGGGAA
ACCAGAGGAG
CTCTCTCGAC
GCAGGACTCG
GCTTGCTGAA
GCGCGCACGG
CAAGAGGCGA
GGGGCGGCGA
CTGGTGAGTA
CGCCAAAAAT
TTTGACTAGC
GGAGGCTAGA
AGGAGAGAGA
TGGGTGCGAG
AGCGTCAGTA
TTAAGCGGGG
GAGAATTAGA
TCGCGATGGG
AAAAAATTCG
GTTAAGGCCA
GGGGGAAAGA
AAAAATATAA
ATTAAAACAT
ATAGTATGGG
CAAGCAGGGA
GCTAGAACGA
TTCGCAGTTA
ATCCTGGCCT
GTTAGAAACA
TCAGAAGGCT
GTAGACAAAT
ACTGGGACAG
CTACAACCAT
CCCTTCAGAC
AGGATCAGAA
GAACTTAGAT
CATTATATAA
TACAGTAGCA
ACCCTCTATT
GTGTGCATCA
AAGGATAGAG
ATAAAAGACA
CCAAGGAAGC
TTTAGACAAG
ATAGAGGAAG
AGCAAAACAA
AAGTAAGACC
ACCGCACAGC
AAGCGGCCGG
CCGCTGATCT
TCAGACCTGG
AGGAGGAGAT
ATGAGGGACA
ATTGGAGAAG
TGAATTATAT
AAATATAAAG
TAGTAAAAAT
TGAACCATTA
GGAGTAGCAC
CCACCAAGGC
AAAGAGAAGA
GTGGTGCAGA
GAGAAAAAAG
AGCAGTGGGA
ATAGGAGCTT
TGTTCCTTGG
GTTCTTGGGA
GCAGCAGGAA
GCACTATGGG
CGCAGCGTCA
ATGACGCTGA
CGGTACAGGC
CAGACAATTA
TTGTCTGGTA
TAGTGCAGCA
GCAGAACAAT
TTGCTGAGGG
CTATTGAGGC
GCAACAGCAT
CTGTTGCAAC
TCACAGTCTG
GGGCATCAAG
CAGCTCCAGG
CAAGAATCCT
GGCTGTGGAA
AGATACCTAA
AGGATCAACA
GCTCCTGGGG
ATTTGGGGTT
GCTCTGGAAA
ACTCATTTGC
ACCACTGCTG
TGCCTTGGAA
TGCTAGTTGG
AGTAATAAAT
CTCTGGAACA
GATTTGGAAT
CACACGACCT
GGATGGAGTG
GGACAGAGAA
ATTAACAATT
ACACAAGCTT
AATACACTCC
TTAATTGAAG
AATCGCAAAA
CCAGCAAGAA
AAGAATGAAC
AAGAATTATT
GGAATTAGAT
AAATGGGCAA
GTTTGTGGAA
TTGGTTTAAC
ATAACAAATT
GGCTGTGGTA
TATAAAATTA
TTCATAATGA
TAGTAGGAGG
CTTGGTAGGT
TTAAGAATAG
TTTTTGCTGT
ACTTTCTATA
GTGAATAGAG
TTAGGCAGGG
ATATTCACCA
TTATCGTTTC
AGACCCACCT
CCCAACCCCG
AGGGGACCCG
ACAGGCCCGA
AGGAATAGAA
GAAGAAGGTG
GAGAGAGAGA
CAGAGACAGA
TCCATTCGAT
TAGTGAACGG
ATCTCGACGG
TATCGCCGAA
TTGACAAATG
GCAGTATTCA
TCCACAATTT
TAAAAGAAAA
GGGGGGATTG
GGGGGTACAG
TGCAGGGGAA
AGAATAGTAG
ACATAATAGC
AACAGACATA
CAAACTAAAG
AATTACAAAA
ACAAATTACA
AAAATTCAAA
ATTTTCGGGT
TTATTACAGG
GACAGCAGAG
ATCCAGTTTG
GACTAGCGAT
GCTAGCTGAT
GCGGGCCCTA
GTTATTAATA
GTAATCAATT
ACGGGGTCAT
TAGTTCATAG
CCCATATATG
GAGTTCCGCG
TTACATAACT
TACGGTAAAT
GGCCCGCCTG
GCTGACCGCC
CAACGACCCC
CGCCCATTGA
CGTCAATAAT
GACGTATGTT
CCCATAGTAA
CGCCAATAGG
GACTTTCCAT
TGACGTCAAT
GGGTGGAGTA
TTTACGGTAA
ACTGCCCACT
TGGCAGTACA
TCAAGTGTAT
CATATGCCAA
GTACGCCCCC
TATTGACGTC
AATGACGGTA
AATGGCCCGC
CTGGCATTAT
GCCCAGTACA
TGACCTTATG
GGACTTTCCT
ACTTGGCAGT
ACATCTACGT
ATTAGTCATC
GCTATTACCA
TGGTGATGCG
GTTTTGGCAG
TACATCAATG
GGCGTGGATA
GCGGTTTGAC
TCACGGGGAT
TTCCAAGTCT
CCACCCCATT
GACGTCAATG
GGAGTTTGTT
TTGGCACCAA
AATCAACGGG
ACTTTCCAAA
ATGTCGTAAC
AACTCCGCCC
CATTGACGCA
AATGGGCGGT
AGGCGTGTAC
GGTGGGAGGT
CTATATAAGC
AGAGCTCTCC
CTATCAGTGA
TAGAGATCTC
CCTATCAGTG
ATAGAGATCG
TCGACGAGCT
CGTTTAGTGA
ACCGTCAGAT
CGCCTGGAGA
CGCCATCCAC
GCTGTTTTGA
CCTCCATAGA
AGACACCGGG
ACCGATCCAG
CCTACCGGTT
AAGGTGAACG
CGTGCAGGCT
GGCGCCACCA
TGGCCCCTAA
AAAGAAACGC
AAGGTCGAAT
TCGGAAGCGG
AAGCGACAAG
AAGTACTCCA
TTGGGCTCGC
TATCGGTACC
AACAGCGTCG
GCTGGGCCGT
CATTACGGAC
GAGTACAAGG
TGCCGAGCAA
AAAATTCAAA
GTTCTGGGCA
ATACCGATCG
CCACAGCATA
AAGAAGAACC
TCATTGGAGC
CCTCCTGTTC
GACTCCGGGG
AGACGGCCGA
AGCCACGCGG
CTCAAAAGAA
CAGCACGGCG
CAGATATACC
CGCAGAAAGA
ATCGGATCTG
CTACCTGCAG
GAGATCTTTA
GTAATGAGAT
GGCTAAGGTG
GATGACTCTT
TCTTCCATAG
GCTGGAGGAG
TCCTTTTTGG
TGGAGGAGGA
TAAAAAGCAC
GAGCGCCACC
CAATCTTTGG
CAATATCGTG
GACGAGGTGG
CGTACCATGA
AAAGTACCCA
ACCATATATC
ATCTGAGGAA
GAAGCTGGTA
GACAGTACTG
ATAAGGCTGA
CTTGCGGTTG
ATCTATCTCG
CGCTGGCGCA
CATGATCAAA
TTTCGGGGAC
ACTTCCTCAT
CGAGGGGGAC
CTGAACCCAG
ACAACAGCGA
TGTCGACAAA
CTCTTTATCC
AACTGGTTCA
GACTTACAAT
CAGCTTTTCG
AGGAGAACCC
GATCAACGCA
TCCGGCGTTG
ACGCCAAAGC
AATCCTGAGC
GCTAGGCTGT
CCAAATCCCG
GCGGCTCGAA
AACCTCATCG
CACAGCTCCC
TGGGGAGAAG
AAGAACGGCC
TGTTTGGTAA
TCTTATCGCC
CTGTCACTCG
GGCTGACCCC
CAACTTTAAA
TCTAACTTCG
ACCTGGCCGA
AGATGCCAAG
CTGCAACTGA
GCAAAGACAC
CTACGATGAT
GATCTCGACA
ATCTGCTGGC
CCAGATCGGC
GACCAGTACG
CAGACCTTTT
TTTGGCGGCA
AAGAACCTGT
CAGACGCCAT
TCTGCTGAGT
GATATTCTGC
GAGTGAACAC
GGAGATCACC
AAAGCTCCGC
TGAGCGCTAG
TATGATCAAG
CGCTATGATG
AGCACCACCA
AGACTTGACT
TTGCTGAAGG
CCCTTGTCAG
ACAGCAACTG
CCTGAGAAGT
ACAAGGAAAT
TTTCTTCGAT
CAGTCTAAAA
ATGGCTACGC
CGGATACATT
GACGGCGGAG
CAAGCCAGGA
GGAATTTTAC
AAATTTATTA
AGCCCATCTT
GGAAAAAATG
GACGGCACCG
AGGAGCTGCT
GGTAAAGCTG
AACAGAGAAG
ATCTGTTGCG
CAAACAGCGC
ACTTTCGACA
ATGGAAGCAT
CCCCCACCAG
ATTCACCTGG
GCGAACTGCA
CGCTATCCTC
AGGCGGCAAG
AGGATTTCTA
CCCCTTTTTG
AAAGATAACA
GGGAAAAGAT
TGAGAAAATC
CTCACATTTC
GGATACCCTA
CTATGTAGGC
CCCCTCGCTC
GGGGAAATTC
CAGATTCGCG
TGGATGACTC
GCAAATCAGA
AGAGACCATC
ACTCCCTGGA
ACTTCGAGGA
AGTCGTGGAT
AAGGGGGCCT
CTGCCCAGTC
CTTCATCGAA
AGGATGACTA
ACTTTGATAA
AAATCTGCCT
AACGAAAAGG
TGCTTCCTAA
ACACTCTCTG
CTGTACGAGT
ACTTCACAGT
TTATAACGAG
CTCACCAAGG
TCAAATACGT
CACAGAAGGG
ATGAGAAAGC
CAGCATTCCT
GTCTGGAGAG
CAGAAGAAAG
CTATCGTGGA
CCTCCTCTTC
AAGACGAACC
GGAAAGTTAC
CGTGAAACAG
CTCAAAGAAG
ACTATTTCAA
AAAGATTGAA
TGTTTCGACT
CTGTTGAAAT
CAGCGGAGTG
GAGGATCGCT
TCAACGCATC
CCTGGGAACG
TATCACGATC
TCCTGAAAAT
CATTAAAGAC
AAGGACTTCC
TGGACAATGA
GGAGAACGAG
GACATTCTTG
AGGACATTGT
CCTCACCCTT
ACGTTGTTTG
AAGATAGGGA
GATGATTGAA
GAACGCTTGA
AAACTTACGC
TCATCTCTTC
GACGACAAAG
TCATGAAACA
GCTCAAGAGA
CGCCGATATA
CAGGATGGGG
GCGGCTGTCA
AGAAAACTGA
TCAATGGCAT
CCGAGACAAG
CAGAGTGGAA
AGACAATCCT
GGATTTTCTT
AAGTCCGATG
GATTTGCCAA
CCGGAACTTC
ATGCAGTTGA
TCCATGATGA
CTCTCTCACC
TTTAAGGAGG
ACATCCAGAA
AGCACAAGTT
TCTGGCCAGG
GGGACAGTCT
TCACGAGCAC
ATCGCTAATC
TTGCAGGTAG
CCCAGCTATC
AAAAAGGGAA
TACTGCAGAC
CGTTAAGGTC
GTGGATGAAC
TCGTCAAAGT
AATGGGAAGG
CATAAGCCCG
AGAATATCGT
TATCGAGATG
GCCCGAGAGA
ACCAAACTAC
CCAGAAGGGA
CAGAAGAACA
GTAGGGAAAG
GATGAAGAGG
ATTGAAGAGG
GTATAAAAGA
ACTGGGGTCC
CAAATCCTTA
AGGAACACCC
AGTTGAAAAC
ACCCAGCTTC
AGAATGAGAA
GCTCTACCTG
TACTACCTGC
AGAACGGCAG
GGACATGTAC
GTGGATCAGG
AACTGGACAT
CAACCGGTTG
TCCGACTACG
ACGTGGATGC
TATCGTGCCC
CAAAGCTTTC
TCAAAGATGA
TTCTATTGAT
AATAAAGTGT
TGACAAGATC
CGATAAAAAT
AGAGGGAAGA
GTGATAACGT
CCCCTCAGAA
GAAGTTGTCA
AGAAAATGAA
AAATTATTGG
CGGCAGCTGC
TGAACGCCAA
ACTGATCACA
CAACGGAAGT
TCGATAATCT
GACTAAGGCT
GAACGAGGTG
GCCTGTCTGA
GTTGGATAAA
GCCGGCTTCA
TCAAAAGGCA
GCTTGTTGAG
ACACGCCAGA
TCACCAAGCA
CGTGGCCCAA
ATTCTCGATT
CACGCATGAA
CACCAAGTAC
GATGAAAATG
ACAAACTGAT
TCGAGAGGTG
AAAGTTATTA
CTCTGAAGTC
TAAGCTGGTC
TCAGATTTCA
GAAAGGACTT
TCAGTTTTAT
AAGGTGAGAG
AGATCAACAA
TTACCACCAT
GCGCATGATG
CCTACCTGAA
TGCAGTGGTA
GGCACTGCAC
TTATCAAAAA
ATATCCCAAG
CTGGAATCTG
AATTTGTTTA
CGGAGACTAT
AAAGTGTACG
ATGTTAGGAA
AATGATCGCA
AAGTCTGAGC
AGGAAATAGG
CAAGGCCACC
GCTAAGTACT
TCTTTTACAG
CAATATTATG
AATTTTTTCA
AGACCGAGAT
TACACTGGCC
AATGGAGAGA
TTCGGAAGCG
ACCACTTATC
GAAACAAACG
GAGAAACAGG
AGAAATCGTG
TGGGACAAGG
GTAGGGATTT
CGCGACAGTC
CGCAAGGTCC
TGTCCATGCC
GCAGGTGAAC
ATCGTTAAAA
AGACCGAAGT
ACAGACCGGA
GGCTTCTCCA
AGGAAAGTAT
CCTCCCGAAA
AGGAACAGCG
ACAAGCTGAT
CGCACGCAAA
AAAGATTGGG
ACCCCAAGAA
ATACGGCGGA
TTCGATTCTC
CTACAGTCGC
TTACAGTGTA
CTGGTTGTGG
CCAAAGTGGA
GAAAGGGAAG
TCTAAAAAAC
TCAAAAGCGT
CAAGGAACTG
CTGGGCATCA
CAATCATGGA
GCGATCCAGC
TTCGAGAAAA
ACCCCATCGA
CTTTCTCGAA
GCGAAAGGAT
ATAAAGAGGT
CAAAAAAGAC
CTCATCATTA
AGCTGCCCAA
GTACTCTCTC
TTTGAGCTTG
AAAACGGCCG
GAAACGAATG
CTCGCTAGTG
CGGGCGAGCT
GCAGAAAGGT
AACGAGCTGG
CACTGCCCTC
TAAATACGTT
AATTTCTTGT
ATCTGGCCAG
CCACTATGAA
AAGCTCAAAG
GGTCTCCCGA
AGATAATGAG
CAGAAGCAGC
TGTTCGTGGA
ACAACACAAA
CACTACCTTG
ATGAGATCAT
CGAGCAAATA
AGCGAGTTCT
CCAAAAGAGT
GATCCTCGCC
GACGCTAACC
TCGATAAGGT
GCTTTCTGCT
TACAATAAGC
ACAGGGATAA
GCCCATCAGG
GAGCAGGCAG
AAAACATTAT
CCACTTGTTT
ACTCTGACCA
ACTTGGGCGC
GCCTGCAGCC
TTCAAGTACT
TCGACACCAC
CATAGACAGA
AAGCGGTACA
CCTCTACAAA
GGAGGTCCTG
GACGCCACAC
TGATTCATCA
GTCAATTACG
GGGCTCTATG
AAACAAGAAT
CGACCTCTCT
CAGCTCGGTG
GAGACGGCTC
TACTAGTGGC
TCTCCCAAGA
AGAAGAGGAA
GGTAGGATCC
GGAAGTATGC
GTAAAGGCGA
AGAGCTGTTC
ACTGGTGTCG
TCCCTATTCT
GGTGGAACTG
GATGGTGATG
TCAACGGTCA
TAAGTTTTCC
GTGCGTGGCG
AGGGTGAAGG
TGACGCAACT
AATGGTAAAC
TGACGCTGAA
GTTCATCTGT
ACTACTGGTA
AACTGCCGGT
ACCTTGGCCG
ACTCTGGTAA
CGACGCTGAC
TTATGGTGTT
CAGTGCTTTG
CTCGTTATCC
GGACCATATG
AAGCAGCATG
ACTTCTTCAA
GTCCGCCATG
CCGGAAGGCT
ATGTGCAGGA
ACGCACGATT
TCCTTTAAGG
ATGACGGCAC
GTACAAAACG
CGTGCGGAAG
TGAAATTTGA
AGGCGATACC
CTGGTAAACC
GCATTGAGCT
GAAAGGCATT
GACTTTAAAG
AAGACGGCAA
TATCCTGGGC
CATAAGCTGG
AATACAATTT
TAACAGCCAC
AATGTTTACA
TCACCGCCGA
TAAACAAAAA
AATGGCATTA
AAGCGAATTT
TAAAATTCGC
CACAACGTGG
AGGATGGCAG
CGTGCAGCTG
GCTGATCACT
ACCAGCAAAA
CACTCCAATC
GGTGATGGTC
CTGTTCTGCT
GCCAGACAAT
CACTATCTGA
GCACGCAAAG
CGTTCTGTCT
AAAGATCCGA
ACGAGAAACG
CGATCATATG
GTTCTGCTGG
AGTTCGTAAC
CGCAGCGGGC
ATCACGCATG
GTATGGATGA
ACTGTACAAA
TCTGGCGGCC
GCTCTGGCAT
GCGTAAAGGC
GAAGAGCTGT
TCACTGGTGT
CGTCCCTATT
CTGGTGGAAC
TGGATGGTGA
TGTCAACGGT
CATAAGTTTT
CCGTGCGTGG
CGAGGGTGAA
GGTGACGCAA
CTAATGGTAA
ACTGACGCTG
AAGTTCATCT
GTACTACTGG
TAAACTGCCG
GTACCTTGGC
CGACTCTGGT
AACGACGCTG
ACTTATGGTG
TTCAGTGCTT
TGCTCGTTAT
CCGGACCATA
TGAAGCAGCA
TGACTTCTTC
AAGTCCGCCA
TGCCGGAAGG
CTATGTGCAG
GAACGCACGA
TTTCCTTTAA
GGATGACGGC
ACGTACAAAA
CGCGTGCGGA
AGTGAAATTT
GAAGGCGATA
CCCTGGTAAA
CCGCATTGAG
CTGAAAGGCA
TTGACTTTAA
AGAAGACGGC
AATATCCTGG
GCCATAAGCT
GGAATACAAT
TTTAACAGCC
ACAATGTTTA
CATCACCGCC
GATAAACAAA
AAAATGGCAT
TAAAGCGAAT
TTTAAAATTC
GCCACAACGT
GGAGGATGGC
AGCGTGCAGC
TGGCTGATCA
CTACCAGCAA
AACACTCCAA
TCGGTGATGG
TCCTGTTCTG
CTGCCAGACA
ATCACTATCT
GAGCACGCAA
AGCGTTCTGT
CTAAAGATCC
GAACGAGAAA
CGCGATCATA
TGGTTCTGCT
GGAGTTCGTA
ACCGCAGCGG
GCATCACGCA
TGGTATGGAT
GAACTGTACA
AAGGTAGCAT
CGATGGTAGC
ATGCGTAAAG
GCGAAGAGCT
GTTCACTGGT
GTCGTCCCTA
TTCTGGTGGA
ACTGGATGGT
GATGTCAACG
GTCATAAGTT
TTCCGTGCGT
GGCGAGGGTG
AAGGTGACGC
AACTAATGGT
AAACTGACGC
TGAAGTTCAT
CTGTACTACT
GGTAAACTGC
CGGTACCTTG
GCCGACTCTG
GTAACGACGC
TGACTTATGG
TGTTCAGTGC
TTTGCTCGTT
ATCCGGACCA
TATGAAGCAG
CATGACTTCT
TCAAGTCCGC
CATGCCGGAA
GGCTATGTGC
AGGAACGCAC
GATTTCCTTT
AAGGATGACG
GCACGTACAA
AACGCGTGCG
GAAGTGAAAT
TTGAAGGCGA
TACCCTGGTA
AACCGCATTG
AGCTGAAAGG
CATTGACTTT
AAAGAAGACG
GCAATATCCT
GGGCCATAAG
CTGGAATACA
ATTTTAACAG
CCACAATGTT
TACATCACCG
CCGATAAACA
AAAAAATGGC
ATTAAAGCGA
ATTTTAAAAT
TCGCCACAAC
GTGGAGGATG
GCAGCGTGCA
GCTGGCTGAT
CACTACCAGC
AAAACACTCC
AATCGGTGAT
GGTCCTGTTC
TGCTGCCAGA
CAATCACTAT
CTGAGCACGC
AAAGCGTTCT
GTCTAAAGAT
CCGAACGAGA
AACGCGATCA
TATGGTTCTG
CTGGAGTTCG
TAACCGCAGC
GGGCATCACG
CATGGTATGG
ATGAACTGTA
CAAACTCGAG
TAGGGTAATC
TAGATAATCA
ACCTCTGGAT
TACAAAATTT
GTGAAAGATT
GACTGGTATT
CTTAACTATG
TTGCTCCTTT
TACGCTATGT
GGATACGCTG
CTTTAATGCC
TTTGTATCAT
GCTATTGCTT
CCCGTATGGC
TTTCATTTTC
TCCTCCTTGT
ATAAATCCTG
GTTGCTGTCT
CTTTATGAGG
AGTTGTGGCC
CGTTGTCAGG
CAACGTGGCG
TGGTGTGCAC
TGTGTTTGCT
GACGCAACCC
CCACTGGTTG
GGGCATTGCC
ACCACCTGTC
AGCTCCTTTC
CGGGACTTTC
GCTTTCCCCC
TCCCTATTGC
CACGGCGGAA
CTCATCGCCG
CCTGCCTTGC
CCGCTGCTGG
ACAGGGGCTC
GGCTGTTGGG
CACTGACAAT
TCCGTGGTGT
TGTCGGGGAA
ATCATCGTCC
TTTCCTTGGC
TGCTCGCCTG
TGTTGCCACC
TGGATTCTGC
GCGGGACGTC
CTTCTGCTAC
GTCCCTTCGG
CCCTCAATCC
AGCGGACCTT
CCTTCCCGCG
GCCTGCTGCC
GGCTCTGCGG
CCTCTTCCGC
GTCTTCGCCT
TCGCCCTCAG
ACGAGTCGGA
TCTCCCTTTG
GGCCGCCTCC
CCGCCTGAGA
TCCTTTAAGA
CCAATGACTT
ACAAGGCAGC
TGTAGATCTT
AGCCACTTTT
TAAAAGAAAA
GGGGGGACTG
GAAGGGCTAA
TTCACTCCCA
ACGAAGACAA
GATCTGCTTT
TTGCTTGTAC
TGGGTCTCTC
TGGTTAGACC
AGATCTGAGC
CTGGGAGCTC
TCTGGCTAAC
TAGGGAACCC
ACTGCTTAAG
CCTCAATAAA
GCTTGCCTTG
AGTGCTTCAA
GTAGTGTGTG
CCCGTCTGTT
GTGTGACTCT
GGTAACTAGA
GATCCCTCAG
ACCCTTTTAG
TCAGTGTGGA
AAATCTCTAG
CAGTAGTAGT
TCATGTCATC
TTATTATTCA
GTATTTATAA
CTTGCAAAGA
AATGAATATC
AGAGAGTGAG
AGGCCCGGGT
TAATTAAGGA
AAGGGCTAGA
TCATTCTTGA
AGACGAAAGG
GCCTCGTGAT
ACGCCTATTT
TTATAGGTTA
ATGTCATGAT
AATAATGGTT
TCTTAGACGT
CAGGTGGCAC
TTTTCGGGGA
AATGTGCGCG
GAACCCCTAT
TTGTTTATTT
TTCTAAATAC
ATTCAAATAT
GTATCCGCTC
ATGAGACAAT
AACCCTGATA
AATGCTTCAA
TAATATTGAA
AAAGGAAGAG
TATGAGTATT
CAACATTTCC
GTGTCGCCCT
TATTCCCTTT
TTTGCGGCAT
TTTGCCTTCC
TGTTTTTGCT
CACCCAGAAA
CGCTGGTGAA
AGTAAAAGAT
GCTGAAGATC
AGTTGGGTGC
ACGAGTGGGT
TACATCGAAC
TGGATCTCAA
CAGCGGTAAG
ATCCTTGAGA
GTTTTCGCCC
CGAAGAACGT
TTTCCAATGA
TGAGCACTTT
TAAAGTTCTG
CTATGTGGCG
CGGTATTATC
CCGTGTTGAC
GCCGGGCAAG
AGCAACTCGG
TCGCCGCATA
CACTATTCTC
AGAATGACTT
GGTTGAGTAC
TCACCAGTCA
CAGAAAAGCA
TCTTACGGAT
GGCATGACAG
TAAGAGAATT
ATGCAGTGCT
GCCATAACCA
TGAGTGATAA
CACTGCGGCC
AACTTACTTC
TGACAACGAT
CGGAGGACCG
AAGGAGCTAA
CCGCTTTTTT
GCACAACATG
GGGGATCATG
TAACTCGCCT
TGATCGTTGG
GAACCGGAGC
TGAATGAAGC
CATACCAAAC
GACGAGCGTG
ACACCACGAT
GCCTGTAGCA
ATGGCAACAA
CGTTGCGCAA
ACTATTAACT
GGCGAACTAC
TTACTCTAGC
TTCCCGGCAA
CAATTAATAG
ACTGGATGGA
GGCGGATAAA
GTTGCAGGAC
CACTTCTGCG
CTCGGCCCTT
CCGGCTGGCT
GGTTTATTGC
TGATAAATCT
GGAGCCGGTG
AGCGTGGGTC
TCGCGGTATC
ATTGCAGCAC
TGGGGCCAGA
TGGTAAGCCC
TCCCGTATCG
TAGTTATCTA
CACGACGGGG
AGTCAGGCAA
CTATGGATGA
ACGAAATAGA
CAGATCGCTG
AGATAGGTGC
CTCACTGATT
AAGCATTGGT
AACTGTCAGA
CCAAGTTTAC
TCATATATAC
TTTAGATTGA
TTTAAAACTT
CATTTTTAAT
TTAAAAGGAT
CTAGGTGAAG
ATCCTTTTTG
ATAATCTCAT
GACCAAAATC
CCTTAACGTG
AGTTTTCGTT
CCACTGAGCG
TCAGACCCCG
TAGAAAAGAT
CAAAGGATCT
TCTTGAGATC
CTTTTTTTCT
GCGCGTAATC
TGCTGCTTGC
AAACAAAAAA
ACCACCGCTA
CCAGCGGTGG
TTTGTTTGCC
GGATCAAGAG
CTACCAACTC
TTTTTCCGAA
GGTAACTGGC
TTCAGCAGAG
CGCAGATACC
AAATACTGTT
CTTCTAGTGT
AGCCGTAGTT
AGGCCACCAC
TTCAAGAACT
CTGTAGCACC
GCCTACATAC
CTCGCTCTGC
TAATCCTGTT
ACCAGTGGCT
GCTGCCAGTG
GCGATAAGTC
GTGTCTTACC
GGGTTGGACT
CAAGACGATA
GTTACCGGAT
AAGGCGCAGC
GGTCGGGCTG
AACGGGGGGT
TCGTGCACAC
AGCCCAGCTT
GGAGCGAACG
ACCTACACCG
AACTGAGATA
CCTACAGCGT
GAGCTATGAG
AAAGCGCCAC
GCTTCCCGAA
GGGAGAAAGG
CGGACAGGTA
TCCGGTAAGC
GGCAGGGTCG
GAACAGGAGA
GCGCACGAGG
GAGCTTCCAG
GGGGAAACGC
CTGGTATCTT
TATAGTCCTG
TCGGGTTTCG
CCACCTCTGA
CTTGAGCGTC
GATTTTTGTG
ATGCTCGTCA
GGGGGGCGGA
GCCTATGGAA
AAACGCCAGC
AACGCGGCCT
TTTTACGGTT
CCTGGCCTTT
TGCTGGCCTT
TTGCTCACAT
GTTCTTTCCT
GCGTTATCCC
CTGATTCTGT
GGATAACCGT
ATTACCGCCT
TTGAGTGAGC
TGATACCGCT
CGCCGCAGCC
GAACGACCGA
GCGCAGCGAG
TCAGTGAGCG
AGGAAGCGGA
AGAGCGCCCA
ATACGCAAAC
CGCCTCTCCC
CGCGCGTTGG
CCGATTCATT
AATGCAGCAA
GCTCATGGCT
GACTAATTTT
TTTTATTTAT
GCAGAGGCCG
AGGCCGCCTC
GGCCTCTGAG
CTATTCCAGA
AGTAGTGAGG
AGGCTTTTTT
GGAGGCCTAG
GCTTTTGCAA
AAAGCTCCCC
GTGGCACGAC
AGGTTTCCCG
ACTGGAAAGC
GGGCAGTGAG
CGCAACGCAA
TTAATGTGAG
TTAGCTCACT
CATTAGGCAC
CCCAGGCTTT
ACACTTTATG
CTTCCGGCTC
GTATGTTGTG
TGGAATTGTG
AGCGGATAAC
AATTTCACAC
AGGAAACAGC
TATGACATGA
TTACGAATTT
CACAAATAAA
GCATTTTTTT
CACTGCATTC
TAGTTGTGGT
TTGTCCAAAC
TCATCAATGT
ATCTTATCAT
GTCTGGATCA
ACTGGATAAC
TCAAGCTAAC
CAAAATCATC
CCAAACTTCC
CACCCCATAC
CCTATTACCA
CTGCCAATTA
CCTGTGGTTT
CATTTACTCT
AAACCTGTGA
TTCCTCTGAA
TTATTTTCAT
TTTAAAGAAA
TTGTATTTGT
TAAATATGTA
CTACAAACTT
AGTAGT