收藏网址 | 中文

pCMV-NBN(human)-3×FLAG-EGFP-puro

产品货号:P59067

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pCMV-NBN(human)-3×FLAG-EGFP-puro
别称: NM_002485.5;AT-V1; AT-V2; ATV; NBS; NBS1; P95;
基因长: 2262bp
质粒宿主: 哺乳细胞
质粒用途: 蛋白表达
片段类型: ORF
片段物种:
原核抗性: Amp
筛选标记: Puro
荧光标记: 绿色
启动子: CMV
复制子: pUC
拷贝数:
感受态: DH5a
温度: 37度
背景载体:
正向引物: CMV-F
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

CGTTACATAA

CTTACGGTAA

ATGGCCCGCC

TGGCTGACCG

CCCAACGACC

CCCGCCCATT

61

GACGTCAATA

ATGACGTATG

TTCCCATAGT

AACGCCAATA

GGGACTTTCC

ATTGACGTCA

121

ATGGGTGGAG

TATTTACGGT

AAACTGCCCA

CTTGGCAGTA

CATCAAGTGT

ATCATATGCC

181

AAGTACGCCC

CCTATTGACG

TCAATGACGG

TAAATGGCCC

GCCTGGCATT

ATGCCCAGTA

241

CATGACCTTA

TGGGACTTTC

CTACTTGGCA

GTACATCTAC

GTATTAGTCA

TCGCTATTAC

301

CATGGTGATG

CGGTTTTGGC

AGTACATCAA

TGGGCGTGGA

TAGCGGTTTG

ACTCACGGGG

361

ATTTCCAAGT

CTCCACCCCA

TTGACGTCAA

TGGGAGTTTG

TTTTGGCACC

AAAATCAACG

421

GGACTTTCCA

AAATGTCGTA

ACAACTCCGC

CCCATTGACG

CAAATGGGCG

GTAGGCGTGT

481

ACGGTGGGAG

GTCTATATAA

GCAGAGCTGG

TTTAGTGAAC

CGTCAGATCC

GCTAGCGCTA

541

CCGGACTCAG

ATCTCGAGCC

ACCATGTGGA

AACTGCTGCC

CGCCGCGGGC

CCGGCAGGAG

601

GAGAACCATA

CAGACTTTTG

ACTGGCGTTG

AGTACGTTGT

TGGAAGGAAA

AACTGTGCCA

661

TTCTGATTGA

AAATGATCAG

TCGATCAGCC

GAAATCATGC

TGTGTTAACT

GCTAACTTTT

721

CTGTAACCAA

CCTGAGTCAA

ACAGATGAAA

TCCCTGTATT

GACATTAAAA

GATAATTCTA

781

AGTATGGTAC

CTTTGTTAAT

GAGGAAAAAA

TGCAGAATGG

CTTTTCCCGA

ACTTTGAAGT

841

CGGGGGATGG

TATTACTTTT

GGAGTGTTTG

GAAGTAAATT

CAGAATAGAG

TATGAGCCTT

901

TGGTTGCATG

CTCTTCTTGT

TTAGATGTCT

CTGGGAAAAC

TGCTTTAAAT

CAAGCTATAT

961

TGCAACTTGG

AGGATTTACT

GTAAACAATT

GGACAGAAGA

ATGCACTCAC

CTTGTCATGG

1021

TATCAGTGAA

AGTTACCATT

AAAACAATAT

GTGCACTCAT

TTGTGGACGT

CCAATTGTAA

1081

AGCCAGAATA

TTTTACTGAA

TTCCTGAAAG

CAGTTGAGTC

CAAGAAGCAG

CCTCCACAAA

1141

TTGAAAGTTT

TTACCCACCT

CTTGATGAAC

CATCTATTGG

AAGTAAAAAT

GTTGATCTGT

1201

CAGGACGGCA

GGAAAGAAAA

CAAATCTTCA

AAGGGAAAAC

ATTTATATTT

TTGAATGCCA

1261

AACAGCATAA

GAAATTGAGT

TCCGCAGTTG

TCTTTGGAGG

TGGGGAAGCT

AGGTTGATAA

1321

CAGAAGAGAA

TGAAGAAGAA

CATAATTTCT

TTTTGGCTCC

GGGAACGTGT

GTTGTTGATA

1381

CAGGAATAAC

AAACTCACAG

ACCTTAATTC

CTGACTGTCA

GAAGAAATGG

ATTCAGTCAA

1441

TAATGGATAT

GCTCCAAAGG

CAAGGTCTTA

GACCTATTCC

TGAAGCAGAA

ATTGGATTGG

1501

CGGTGATTTT

CATGACTACA

AAGAATTACT

GTGATCCTCA

GGGCCATCCC

AGTACAGGAT

1561

TAAAGACAAC

AACTCCAGGA

CCAAGCCTTT

CACAAGGCGT

GTCAGTTGAT

GAAAAACTAA

1621

TGCCAAGCGC

CCCAGTGAAC

ACTACAACAT

ACGTAGCTGA

CACAGAATCA

GAGCAAGCAG

1681

ATACATGGGA

TTTGAGTGAA

AGGCCAAAAG

AAATCAAAGT

CTCCAAAATG

GAACAAAAAT

1741

TCAGAATGCT

TTCACAAGAT

GCACCCACTG

TAAAGGAGTC

CTGCAAAACA

AGCTCTAATA

1801

ATAATAGTAT

GGTATCAAAT

ACTTTGGCTA

AGATGAGAAT

CCCAAACTAT

CAGCTTTCAC

1861

CAACTAAATT

GCCAAGTATA

AATAAAAGTA

AAGATAGGGC

TTCTCAGCAG

CAGCAGACCA

1921

ACTCCATCAG

AAACTACTTT

CAGCCGTCTA

CCAAAAAAAG

GGAAAGGGAT

GAAGAAAATC

1981

AAGAAATGTC

TTCATGCAAA

TCAGCAAGAA

TAGAAACGTC

TTGTTCTCTT

TTAGAACAAA

2041

CACAACCTGC

TACACCCTCA

TTGTGGAAAA

ATAAGGAGCA

GCATCTATCT

GAGAATGAGC

2101

CTGTGGACAC

AAACTCAGAC

AATAACTTAT

TTACAGATAC

AGATTTAAAA

TCTATTGTGA

2161

AAAATTCTGC

CAGTAAATCT

CATGCTGCAG

AAAAGCTAAG

ATCAAATAAA

AAAAGGGAAA

2221

TGGATGATGT

GGCCATAGAA

GATGAAGTAT

TGGAACAGTT

ATTCAAGGAC

ACAAAACCAG

2281

AGTTAGAAAT

TGATGTGAAA

GTTCAAAAAC

AGGAGGAAGA

TGTCAATGTT

AGAAAAAGGC

2341

CAAGGATGGA

TATAGAAACA

AATGACACTT

TCAGTGATGA

AGCAGTACCA

GAAAGTAGCA

2401

AAATATCTCA

AGAAAATGAA

ATTGGGAAGA

AACGTGAACT

CAAGGAAGAC

TCACTATGGT

2461

CAGCTAAAGA

AATATCTAAC

AATGACAAAC

TTCAGGATGA

TAGTGAGATG

CTTCCAAAAA

2521

AGCTGTTATT

GACTGAATTT

AGATCACTGG

TGATTAAAAA

CTCTACTTCC

AGAAATCCAT

2581

CTGGCATAAA

TGATGATTAT

GGTCAACTAA

AAAATTTCAA

GAAATTCAAA

AAGGTCACAT

2641

ATCCTGGAGC

AGGAAAACTT

CCACACATCA

TTGGAGGATC

AGATCTAATA

GCTCATCATG

2701

CTCGAAAGAA

TACAGAACTA

GAAGAGTGGC

TAAGGCAGGA

AATGGAGGTA

CAAAATCAAC

2761

ATGCAAAAGA

AGAGTCTCTT

GCTGATGATC

TTTTTAGATA

CAATCCTTAT

TTAAAAAGGA

2821

GAAGAGACTA

CAAAGACCAT

GACGGTGATT

ATAAAGATCA

TGACATCGAC

TACAAGGATG

2881

ACGATGACAA

GTAGTTCGAA

TTCTGCAGTC

GACGGTACCG

CGGGCCCGGG

ATCGATCCGC

2941

CCCTCTCCCT

CCCCCCCCCC

TAACGTTACT

GGCCGAAGCC

GCTTGGAATA

AGGCCGGTGT

3001

GCGTTTGTCT

ATATGTTATT

TTCCACCATA

TTGCCGTCTT

TTGGCAATGT

GAGGGCCCGG

3061

AAACCTGGCC

CTGTCTTCTT

GACGAGCATT

CCTAGGGGTC

TTTCCCCTCT

CGCCAAAGGA

3121

ATGCAAGGTC

TGTTGAATGT

CGTGAAGGAA

GCAGTTCCTC

TGGAAGCTTC

TTGAAGACAA

3181

ACAACGTCTG

TAGCGACCCT

TTGCAGGCAG

CGGAACCCCC

CACCTGGCGA

CAGGTGCCTC

3241

TGCGGCCAAA

AGCCACGTGT

ATAAGATACA

CCTGCAAAGG

CGGCACAACC

CCAGTGCCAC

3301

GTTGTGAGTT

GGATAGTTGT

GGAAAGAGTC

AAATGGCTCT

CCTCAAGCGT

ATTCAACAAG

3361

GGGCTGAAGG

ATGCCCAGAA

GGTACCCCAT

TGTATGGGAT

CTGATCTGGG

GCCTCGGTAC

3421

ACATGCTTTA

CATGTGTTTA

GTCGAGGTTA

AAAAAACGTC

TAGGCCCCCC

GAACCACGGG

3481

GACGTGGTTT

TCCTTTGAAA

AACACGATGA

TAATATGGCC

ACAACCATGG

TGAGCAAGGG

3541

CGAGGAGCTG

TTCACCGGGG

TGGTGCCCAT

CCTGGTCGAG

CTGGACGGCG

ACGTAAACGG

3601

CCACAAGTTC

AGCGTGTCCG

GCGAGGGCGA

GGGCGATGCC

ACCTACGGCA

AGCTGACCCT

3661

GAAGTTCATC

TGCACCACCG

GCAAGCTGCC

CGTGCCCTGG

CCCACCCTCG

TGACCACCCT

3721

GACCTACGGC

GTGCAGTGCT

TCAGCCGCTA

CCCCGACCAC

ATGAAGCAGC

ACGACTTCTT

3781

CAAGTCCGCC

ATGCCCGAAG

GCTACGTCCA

GGAGCGCACC

ATCTTCTTCA

AGGACGACGG

3841

CAACTACAAG

ACCCGCGCCG

AGGTGAAGTT

CGAGGGCGAC

ACCCTGGTGA

ACCGCATCGA

3901

GCTGAAGGGC

ATCGACTTCA

AGGAGGACGG

CAACATCCTG

GGGCACAAGC

TGGAGTACAA

3961

CTACAACAGC

CACAACGTCT

ATATCATGGC

CGACAAGCAG

AAGAACGGCA

TCAAGGTGAA

4021

CTTCAAGATC

CGCCACAACA

TCGAGGACGG

CAGCGTGCAG

CTCGCCGACC

ACTACCAGCA

4081

GAACACCCCC

ATCGGCGACG

GCCCCGTGCT

GCTGCCCGAC

AACCACTACC

TGAGCACCCA

4141

GTCCGCCCTG

AGCAAAGACC

CCAACGAGAA

GCGCGATCAC

ATGGTCCTGC

TGGAGTTCGT

4201

GACCGCCGCC

GGGATCACTC

TCGGCATGGA

CGAGCTGTAC

AAGTCCGGAC

TCAGATCTAG

4261

GAGACGACCT

TCCATGACCG

AGTACAAGCC

CACGGTGCGC

CTCGCCACCC

GCGACGACGT

4321

CCCCCGGGCC

GTACGCACCC

TCGCCGCCGC

GTTCGCCGAC

TACCCCGCCA

CGCGCCACAC

4381

CGTCGACCCG

GACCGCCACA

TCGAGCGGGT

CACCGAGCTG

CAAGAACTCT

TCCTCACGCG

4441

CGTCGGGCTC

GACATCGGCA

AGGTGTGGGT

CGCGGACGAC

GGCGCCGCGG

TGGCGGTCTG

4501

GACCACGCCG

GAGAGCGTCG

AAGCGGGGGC

GGTGTTCGCC

GAGATCGGCC

CGCGCATGGC

4561

CGAGTTGAGC

GGTTCCCGGC

TGGCCGCGCA

GCAACAGATG

GAAGGCCTCC

TGGCGCCGCA

4621

CCGGCCCAAG

GAGCCCGCGT

GGTTCCTGGC

CACCGTCGGC

GTCTCGCCCG

ACCACCAGGG

4681

CAAGGGTCTG

GGCAGCGCCG

TCGTGCTCCC

CGGAGTGGAG

GCGGCCGAGC

GCGCCGGGGT

4741

GCCCGCCTTC

CTGGAGACCT

CCGCGCCCCG

CAACCTCCCC

TTCTACGAGC

GGCTCGGCTT

4801

CACCGTCACC

GCCGACGTCG

AGGTGCCCGA

AGGACCGCGC

ACCTGGTGCA

TGACCCGCAA

4861

GCCCGGTGCG

GGATCCACCG

GATCTAGATA

ACTGATCATA

ATCAGCCATA

CCACATTTGT

4921

AGAGGTTTTA

CTTGCTTTAA

AAAACCTCCC

ACACCTCCCC

CTGAACCTGA

AACATAAAAT

4981

GAATGCAATT

GTTGTTGTTA

ACTTGTTTAT

TGCAGCTTAT

AATGGTTACA

AATAAAGCAA

5041

TAGCATCACA

AATTTCACAA

ATAAAGCATT

TTTTTCACTG

CATTCTAGTT

GTGGTTTGTC

5101

CAAACTCATC

AATGTATCTT

AACGCGATCA

AGCTCCAATT

CGCCCTATAG

TGAGTCGTAT

5161

TACGCGCGCT

CACTGGCCGT

CGTTTTACAA

CGTCGTGACT

GGGAAAACCC

TGGCGTTACC

5221

CAACTTAATC

GCCTTGCAGC

ACATCCCCCT

TTCGCCAGCT

GGCGTAATAG

CGAAGAGGCC

5281

CGCACCGATC

GCCCTTCCCA

ACAGTTGCGC

AGCCTGAATG

GCGAATGGAA

ATTGTAAGCG

5341

TTAATATTTT

GTTAAAATTC

GCGTTAAATT

TTTGTTAAAT

CAGCTCATTT

TTTTAACCAA

5401

TAGGCCGAAA

TCGGCAAAAT

CCCTTATAAA

TCAAAAGAAT

AGACCGAGAT

AGGGTTGAGT

5461

GTTGTTCCAG

TTTGGAACAA

GAGTCCACTA

TTAAAGAACG

TGGACTCCAA

CGTCAAAGGG

5521

CGAAAAACCG

TCTATCAGGG

CGATGGCCCA

CTACGTGAAC

CATCACCCTA

ATCAAGTTTT

5581

TTGGGGTCGA

GGTGCCGTAA

AGCACTAAAT

CGGAACCCTA

AAGGGAGCCC

CCGATTTAGA

5641

GCTTGACGGG

GAAAGCCGGC

GAACGTGGCG

AGAAAGGAAG

GGAAGAAAGC

GAAAGGAGCG

5701

GGCGCTAGGG

CGCTGGCAAG

TGTAGCGGTC

ACGCTGCGCG

TAACCACCAC

ACCCGCCGCG

5761

CTTAATGCGC

CGCTACAGGG

CGCGTCAGGT

GGCACTTTTC

GGGGAAATGT

GCGCGGAACC

5821

CCTATTTGTT

TATTTTTCTA

AATACATTCA

AATATGTATC

CGCTCATGAG

ACAATAACCC

5881

TGATAAATGC

TTCAATAATA

TTGAAAAAGG

AAGAGTATGA

GTATTCAACA

TTTCCGTGTC

5941

GCCCTTATTC

CCTTTTTTGC

GGCATTTTGC

CTTCCTGTTT

TTGCTCACCC

AGAAACGCTG

6001

GTGAAAGTAA

AAGATGCTGA

AGATCAGTTG

GGTGCACGAG

TGGGTTACAT

CGAACTGGAT

6061

CTCAACAGCG

GTAAGATCCT

TGAGAGTTTT

CGCCCCGAAG

AACGTTTTCC

AATGATGAGC

6121

ACTTTTAAAG

TTCTGCTATG

TGGCGCGGTA

TTATCCCGTA

TTGACGCCGG

GCAAGAGCAA

6181

CTCGGTCGCC

GCATACACTA

TTCTCAGAAT

GACTTGGTTG

AGTACTCACC

AGTCACAGAA

6241

AAGCATCTTA

CGGATGGCAT

GACAGTAAGA

GAATTATGCA

GTGCTGCCAT

AACCATGAGT

6301

GATAACACTG

CGGCCAACTT

ACTTCTGACA

ACGATCGGAG

GACCGAAGGA

GCTAACCGCT

6361

TTTTTGCACA

ACATGGGGGA

TCATGTAACT

CGCCTTGATC

GTTGGGAACC

GGAGCTGAAT

6421

GAAGCCATAC

CAAACGACGA

GCGTGACACC

ACGATGCCTG

TAGCAATGGC

AACAACGTTG

6481

CGCAAACTAT

TAACTGGCGA

ACTACTTACT

CTAGCTTCCC

GGCAACAATT

AATAGACTGG

6541

ATGGAGGCGG

ATAAAGTTGC

AGGACCACTT

CTGCGCTCGG

CCCTTCCGGC

TGGCTGGTTT

6601

ATTGCTGATA

AATCTGGAGC

CGGTGAGCGT

GGGTCTCGCG

GTATCATTGC

AGCACTGGGG

6661

CCAGATGGTA

AGCCCTCCCG

TATCGTAGTT

ATCTACACGA

CGGGGAGTCA

GGCAACTATG

6721

GATGAACGAA

ATAGACAGAT

CGCTGAGATA

GGTGCCTCAC

TGATTAAGCA

TTGGTAACTG

6781

TCAGACCAAG

TTTACTCATA

TATACTTTAG

ATTGATTTAA

AACTTCATTT

TTAATTTAAA

6841

AGGATCTAGG

TGAAGATCCT

TTTTGATAAT

CTCATGACCA

AAATCCCTTA

ACGTGAGTTT

6901

TCGTTCCACT

GAGCGTCAGA

CCCCGTAGAA

AAGATCAAAG

GATCTTCTTG

AGATCCTTTT

6961

TTTCTGCGCG

TAATCTGCTG

CTTGCAAACA

AAAAAACCAC

CGCTACCAGC

GGTGGTTTGT

7021

TTGCCGGATC

AAGAGCTACC

AACTCTTTTT

CCGAAGGTAA

CTGGCTTCAG

CAGAGCGCAG

7081

ATACCAAATA

CTGTTCTTCT

AGTGTAGCCG

TAGTTAGGCC

ACCACTTCAA

GAACTCTGTA

7141

GCACCGCCTA

CATACCTCGC

TCTGCTAATC

CTGTTACCAG

TGGCTGCTGC

CAGTGGCGAT

7201

AAGTCGTGTC

TTACCGGGTT

GGACTCAAGA

CGATAGTTAC

CGGATAAGGC

GCAGCGGTCG

7261

GGCTGAACGG

GGGGTTCGTG

CACACAGCCC

AGCTTGGAGC

GAACGACCTA

CACCGAACTG

7321

AGATACCTAC

AGCGTGAGCT

ATGAGAAAGC

GCCACGCTTC

CCGAAGGGAG

AAAGGCGGAC

7381

AGGTATCCGG

TAAGCGGCAG

GGTCGGAACA

GGAGAGCGCA

CGAGGGAGCT

TCCAGGGGGA

7441

AACGCCTGGT

ATCTTTATAG

TCCTGTCGGG

TTTCGCCACC

TCTGACTTGA

GCGTCGATTT

7501

TTGTGATGCT

CGTCAGGGGG

GCGGAGCCTA

TGGAAAAACG

CCAGCAACGC

GGCCTTTTTA

7561

CGGTTCCTGG

CCTTTTGCTG

GCCTTTTGCT

CACATGTTCT

TTCCTGCGTT

ATCCCCTGAT

7621

TCTGTGGATA

ACCGTATTAC

CGCCTTTGAG

TGAGCTGATA

CCGCTCGCCG

CAGCCGAACG

7681

ACCGAGCGCA

GCGAGTCAGT

GAGCGAGGAA

GCGGAAGAGC

GCCCAATACG

CAAACCGCCT

7741

CTCCCCGCGC

GTTGGCCGAT

TCATTAATGC

AGCTGGCACG

ACAGGTTTCC

CGACTGGAAA

7801

GCGGGCAGTG

AGCGCAACGC

AATTAATGTG

AGTTAGCTCA

CTCATTAGGC

ACCCCAGGCT

7861

TTACACTTTA

TGCTTCCGGC

TCGTATGTTG

TGTGGAATTG

TGAGCGGATA

ACAATTTCAC

7921

ACAGGAAACA

GCTATGACCA

TGATTACGCC

AAGCGCGCAA

TTAACCCTCA

CTAAAGGGAA

7981

CAAAAGCTGG

CTAGTGGATC

CCCCGGGCTG

CAGGAATTCG

ATTAATAGTA

ATCAATTACG

8041

GGGTCATTAG

TTCATAGCCC

ATATATGGAG

TTCCG

武汉网监电子标识 Copyright © 武汉淼灵生物科技有限公司版权所有/鄂公网安备42010702000282号 鄂ICP备14012402号-1
访客数:2972992
2.794806s