名称: | pLV3-U6-NQO1(human)-sgRNA3-Cas9-EGFP-Puro-rTetR |
---|---|
别称: | - |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro,Zeo |
荧光标记: | 绿色 |
启动子: | U6 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | U6-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ATGTCGAGTT
TACTCCCTAT
CAGTGATAGA
GAACGATGTC
GAGTTTACTC
CCTATCAGTG
ATAGAGAACG
TATGTCGAGT
TTACTCCCTA
TCAGTGATAG
AGAACGTATG
TCGAGTTTAC
TCCCTATCAG
TGATAGAGAA
CGTATGTCGA
GTTTATCCCT
ATCAGTGATA
GAGAACGTAT
GTCGAGTTTA
CTCCCTATCA
GTGATAGAGA
ACGTATGTCG
AGGTAGGCGT
GTACGGTGGG
AGGCCTATAT
AAGCAGAGCT
CGTTTAGTGA
ACCGTCAGAT
CGCCTGGAGA
ATACCGGTTC
TAGAGCGCTG
CCACCATGGA
CAAGAAGTAC
AGCATCGGCC
TGGACATCGG
CACCAACTCT
GTGGGCTGGG
CCGTGATCAC
CGACGAGTAC
AAGGTGCCCA
GCAAGAAATT
CAAGGTGCTG
GGCAACACCG
ACCGGCACAG
CATCAAGAAG
AACCTGATCG
GAGCCCTGCT
GTTCGACAGC
GGCGAAACAG
CCGAGGCCAC
CCGGCTGAAG
AGAACCGCCA
GAAGAAGATA
CACCAGACGG
AAGAACCGGA
TCTGCTATCT
GCAAGAGATC
TTCAGCAACG
AGATGGCCAA
GGTGGACGAC
AGCTTCTTCC
ACAGACTGGA
AGAGTCCTTC
CTGGTGGAAG
AGGATAAGAA
GCACGAGCGG
CACCCCATCT
TCGGCAACAT
CGTGGACGAG
GTGGCCTACC
ACGAGAAGTA
CCCCACCATC
TACCACCTGA
GAAAGAAACT
GGTGGACAGC
ACCGACAAGG
CCGACCTGCG
GCTGATCTAT
CTGGCCCTGG
CCCACATGAT
CAAGTTCCGG
GGCCACTTCC
TGATCGAGGG
CGACCTGAAC
CCCGACAACA
GCGACGTGGA
CAAGCTGTTC
ATCCAGCTGG
TGCAGACCTA
CAACCAGCTG
TTCGAGGAAA
ACCCCATCAA
CGCCAGCGGC
GTGGACGCCA
AGGCCATCCT
GTCTGCCAGA
CTGAGCAAGA
GCAGACGGCT
GGAAAATCTG
ATCGCCCAGC
TGCCCGGCGA
GAAGAAGAAT
GGCCTGTTCG
GAAACCTGAT
TGCCCTGAGC
CTGGGCCTGA
CCCCCAACTT
CAAGAGCAAC
TTCGACCTGG
CCGAGGATGC
CAAACTGCAG
CTGAGCAAGG
ACACCTACGA
CGACGACCTG
GACAACCTGC
TGGCCCAGAT
CGGCGACCAG
TACGCCGACC
TGTTTCTGGC
CGCCAAGAAC
CTGTCCGACG
CCATCCTGCT
GAGCGACATC
CTGAGAGTGA
ACACCGAGAT
CACCAAGGCC
CCCCTGAGCG
CCTCTATGAT
CAAGAGATAC
GACGAGCACC
ACCAGGACCT
GACCCTGCTG
AAAGCTCTCG
TGCGGCAGCA
GCTGCCTGAG
AAGTACAAAG
AGATTTTCTT
CGACCAGAGC
AAGAACGGCT
ACGCCGGCTA
CATTGACGGC
GGAGCCAGCC
AGGAAGAGTT
CTACAAGTTC
ATCAAGCCCA
TCCTGGAAAA
GATGGACGGC
ACCGAGGAAC
TGCTCGTGAA
GCTGAACAGA
GAGGACCTGC
TGCGGAAGCA
GCGGACCTTC
GACAACGGCA
GCATCCCCCA
CCAGATCCAC
CTGGGAGAGC
TGCACGCCAT
TCTGCGGCGG
CAGGAAGATT
TTTACCCATT
CCTGAAGGAC
AACCGGGAAA
AGATCGAGAA
GATCCTGACC
TTCCGCATCC
CCTACTACGT
GGGCCCTCTG
GCCAGGGGAA
ACAGCAGATT
CGCCTGGATG
ACCAGAAAGA
GCGAGGAAAC
CATCACCCCC
TGGAACTTCG
AGGAAGTGGT
GGACAAGGGC
GCTTCCGCCC
AGAGCTTCAT
CGAGCGGATG
ACCAACTTCG
ATAAGAACCT
GCCCAACGAG
AAGGTGCTGC
CCAAGCACAG
CCTGCTGTAC
GAGTACTTCA
CCGTGTATAA
CGAGCTGACC
AAAGTGAAAT
ACGTGACCGA
GGGAATGAGA
AAGCCCGCCT
TCCTGAGCGG
CGAGCAGAAA
AAGGCCATCG
TGGACCTGCT
GTTCAAGACC
AACCGGAAAG
TGACCGTGAA
GCAGCTGAAA
GAGGACTACT
TCAAGAAAAT
CGAGTGCTTC
GACTCCGTGG
AAATCTCCGG
CGTGGAAGAT
CGGTTCAACG
CCTCCCTGGG
CACATACCAC
GATCTGCTGA
AAATTATCAA
GGACAAGGAC
TTCCTGGACA
ATGAGGAAAA
CGAGGACATT
CTGGAAGATA
TCGTGCTGAC
CCTGACACTG
TTTGAGGACA
GAGAGATGAT
CGAGGAACGG
CTGAAAACCT
ATGCCCACCT
GTTCGACGAC
AAAGTGATGA
AGCAGCTGAA
GCGGCGGAGA
TACACCGGCT
GGGGCAGGCT
GAGCCGGAAG
CTGATCAACG
GCATCCGGGA
CAAGCAGTCC
GGCAAGACAA
TCCTGGATTT
CCTGAAGTCC
GACGGCTTCG
CCAACAGAAA
CTTCATGCAG
CTGATCCACG
ACGACAGCCT
GACCTTTAAA
GAGGACATCC
AGAAAGCCCA
GGTGTCCGGC
CAGGGCGATA
GCCTGCACGA
GCACATTGCC
AATCTGGCCG
GCAGCCCCGC
CATTAAGAAG
GGCATCCTGC
AGACAGTGAA
GGTGGTGGAC
GAGCTCGTGA
AAGTGATGGG
CCGGCACAAG
CCCGAGAACA
TCGTGATCGA
AATGGCCAGA
GAGAACCAGA
CCACCCAGAA
GGGACAGAAG
AACAGCCGCG
AGAGAATGAA
GCGGATCGAA
GAGGGCATCA
AAGAGCTGGG
CAGCCAGATC
CTGAAAGAAC
ACCCCGTGGA
AAACACCCAG
CTGCAGAACG
AGAAGCTGTA
CCTGTACTAC
CTGCAGAATG
GGCGGGATAT
GTACGTGGAC
CAGGAACTGG
ACATCAACCG
GCTGTCCGAC
TACGATGTGG
ACCATATCGT
GCCTCAGAGC
TTTCTGAAGG
ACGACTCCAT
CGACAACAAG
GTGCTGACCA
GAAGCGACAA
GAACCGGGGC
AAGAGCGACA
ACGTGCCCTC
CGAAGAGGTC
GTGAAGAAGA
TGAAGAACTA
CTGGCGGCAG
CTGCTGAACG
CCAAGCTGAT
TACCCAGAGA
AAGTTCGACA
ATCTGACCAA
GGCCGAGAGA
GGCGGCCTGA
GCGAACTGGA
TAAGGCCGGC
TTCATCAAGA
GACAGCTGGT
GGAAACCCGG
CAGATCACAA
AGCACGTGGC
ACAGATCCTG
GACTCCCGGA
TGAACACTAA
GTACGACGAG
AATGACAAGC
TGATCCGGGA
AGTGAAAGTG
ATCACCCTGA
AGTCCAAGCT
GGTGTCCGAT
TTCCGGAAGG
ATTTCCAGTT
TTACAAAGTG
CGCGAGATCA
ACAACTACCA
CCACGCCCAC
GACGCCTACC
TGAACGCCGT
CGTGGGAACC
GCCCTGATCA
AAAAGTACCC
TAAGCTGGAA
AGCGAGTTCG
TGTACGGCGA
CTACAAGGTG
TACGACGTGC
GGAAGATGAT
CGCCAAGAGC
GAGCAGGAAA
TCGGCAAGGC
TACCGCCAAG
TACTTCTTCT
ACAGCAACAT
CATGAACTTT
TTCAAGACCG
AGATTACCCT
GGCCAACGGC
GAGATCCGGA
AGCGGCCTCT
GATCGAGACA
AACGGCGAAA
CCGGGGAGAT
CGTGTGGGAT
AAGGGCCGGG
ATTTTGCCAC
CGTGCGGAAA
GTGCTGAGCA
TGCCCCAAGT
GAATATCGTG
AAAAAGACCG
AGGTGCAGAC
AGGCGGCTTC
AGCAAAGAGT
CTATCCTGCC
CAAGAGGAAC
AGCGATAAGC
TGATCGCCAG
AAAGAAGGAC
TGGGACCCTA
AGAAGTACGG
CGGCTTCGAC
AGCCCCACCG
TGGCCTATTC
TGTGCTGGTG
GTGGCCAAAG
TGGAAAAGGG
CAAGTCCAAG
AAACTGAAGA
GTGTGAAAGA
GCTGCTGGGG
ATCACCATCA
TGGAAAGAAG
CAGCTTCGAG
AAGAATCCCA
TCGACTTTCT
GGAAGCCAAG
GGCTACAAAG
AAGTGAAAAA
GGACCTGATC
ATCAAGCTGC
CTAAGTACTC
CCTGTTCGAG
CTGGAAAACG
GCCGGAAGAG
AATGCTGGCC
TCTGCCGGCG
AACTGCAGAA
GGGAAACGAA
CTGGCCCTGC
CCTCCAAATA
TGTGAACTTC
CTGTACCTGG
CCAGCCACTA
TGAGAAGCTG
AAGGGCTCCC
CCGAGGATAA
TGAGCAGAAA
CAGCTGTTTG
TGGAACAGCA
CAAGCACTAC
CTGGACGAGA
TCATCGAGCA
GATCAGCGAG
TTCTCCAAGA
GAGTGATCCT
GGCCGACGCT
AATCTGGACA
AAGTGCTGTC
CGCCTACAAC
AAGCACCGGG
ATAAGCCCAT
CAGAGAGCAG
GCCGAGAATA
TCATCCACCT
GTTTACCCTG
ACCAATCTGG
GAGCCCCTGC
CGCCTTCAAG
TACTTTGACA
CCACCATCGA
CCGGAAGAGG
TACACCAGCA
CCAAAGAGGT
GCTGGACGCC
ACCCTGATCC
ACCAGAGCAT
CACCGGCCTG
TACGAGACAC
GGATCGACCT
GTCTCAGCTG
GGAGGCGACA
AGCGACCTGC
CGCCACAAAG
AAGGCTGGAC
AGGCTAAGAA
GAAGAAAGAT
TACAAAGACG
ATGACGATAA
GGGATCCGGA
GAGGGCAGAG
GAAGTCTGCT
AACATGCGGT
GACGTCGAGG
AGAATCCTGG
CCCAGTGAGC
AAGGGCGAGG
AGCTGTTCAC
CGGGGTGGTG
CCCATCCTGG
TCGAGCTGGA
CGGCGACGTA
AACGGCCACA
AGTTCAGCGT
GTCCGGCGAG
GGCGAGGGCG
ATGCCACCTA
CGGCAAGCTG
ACCCTGAAGT
TCATCTGCAC
CACCGGCAAG
CTGCCCGTGC
CCTGGCCCAC
CCTCGTGACC
ACCCTGACCT
ACGGCGTGCA
GTGCTTCAGC
CGCTACCCCG
ACCACATGAA
GCAGCACGAC
TTCTTCAAGT
CCGCCATGCC
CGAAGGCTAC
GTCCAGGAGC
GCACCATCTT
CTTCAAGGAC
GACGGCAACT
ACAAGACCCG
CGCCGAGGTG
AAGTTCGAGG
GCGACACCCT
GGTGAACCGC
ATCGAGCTGA
AGGGCATCGA
CTTCAAGGAG
GACGGCAACA
TCCTGGGGCA
CAAGCTGGAG
TACAACTACA
ACAGCCACAA
CGTCTATATC
ATGGCCGACA
AGCAGAAGAA
CGGCATCAAG
GTGAACTTCA
AGATCCGCCA
CAACATCGAG
GACGGCAGCG
TGCAGCTCGC
CGACCACTAC
CAGCAGAACA
CCCCCATCGG
CGACGGCCCC
GTGCTGCTGC
CCGACAACCA
CTACCTGAGC
ACCCAGTCCG
CCCTGAGCAA
AGACCCCAAC
GAGAAGCGCG
ATCACATGGT
CCTGCTGGAG
TTCGTGACCG
CCGCCGGGAT
CACTCTCGGC
ATGGACGAGC
TGTACAAGTA
AGCTAGCATC
CGGGCAGAGC
GCACATCGCC
CACAGTCCCC
GAGAAGTTGG
GGGGAGGGGT
CGGCAATTGA
TCCGGTGCCT
AGAGAAGGTG
GCGCGGGGTA
AACTGGGAAA
GTGATGTCGT
GTACTGGCTC
CGCCTTTTTC
CCGAGGGTGG
GGGAGAACCG
TATATAAGTG
CAGTAGTCGC
CGTGAACGTT
CTTTTTCGCA
ACGGGTTTGC
CGCCAGAACA
CAGACTGCGA
TCGCAATGTA
CAGTATGACC
GAGTACAAGC
CCACGGTGCG
CCTCGCCACC
CGCGACGACG
TCCCCAGGGC
CGTACGCACC
CTCGCCGCCG
CGTTCGCCGA
CTACCCCGCC
ACGCGCCACA
CCGTCGATCC
GGACCGCCAC
ATCGAGCGGG
TCACCGAGCT
GCAAGAACTC
TTCCTCACGC
GCGTCGGGCT
CGACATCGGC
AAGGTGTGGG
TCGCGGACGA
CGGCGCCGCG
GTGGCGGTCT
GGACCACGCC
GGAGAGCGTC
GAAGCGGGGG
CGGTGTTCGC
CGAGATCGGC
CCGCGCATGG
CCGAGTTGAG
CGGTTCCCGG
CTGGCCGCGC
AGCAACAGAT
GGAAGGCCTC
CTGGCGCCGC
ACCGGCCCAA
GGAGCCCGCG
TGGTTCCTGG
CCACCGTCGG
AGTCTCGCCC
GACCACCAGG
GCAAGGGTCT
GGGCAGCGCC
GTCGTGCTCC
CCGGAGTGGA
GGCGGCCGAG
CGCGCCGGGG
TGCCCGCCTT
CCTGGAGACC
TCCGCGCCCC
GCAACCTCCC
CTTCTACGAG
CGGCTCGGCT
TCACCGTCAC
CGCCGACGTC
GAGGTGCCCG
AAGGACCGCG
CACCTGGTGC
ATGACCCGCA
AGCCCGGTGC
CGGTTCCGGC
GCAACAAACT
TCTCTCTGCT
GAAACAAGCC
GGAGATGTCG
AAGAGAATCC
TGGACCGATG
TCTAGACTGG
ACAAGAGCAA
AGTCATAAAC
GGAGCTCTGG
AATTACTCAA
TGGTGTCGGT
ATCGAAGGCC
TGACGACAAG
GAAACTCGCT
CAAAAGCTGG
GAGTTGAGCA
GCCTACCCTG
TACTGGCACG
TGAAGAACAA
GCGGGCCCTG
CTCGATGCCC
TGCCAATCGA
GATGCTGGAC
AGGCATCATA
CCCACTTCTG
CCCCCTGGAA
GGCGAGTCAT
GGCAAGACTT
TCTGCGGAAC
AACGCCAAGT
CATACCGCTG
TGCTCTCCTC
TCACATCGCG
ACGGGGCTAA
AGTGCATCTC
GGCACCCGCC
CAACAGAGAA
ACAGTACGAA
ACCCTGGAAA
ATCAGCTCGC
GTTCCTGTGT
CAGCAAGGCT
TCTCCCTGGA
GAACGCACTG
TACGCTCTGT
CCGCCGTGGG
CCACTTTACA
CTGGGCTGCG
TATTGGAGGA
ACAGGAGCAT
CAAGTAGCAA
AAGAGGAAAG
AGAGACACCT
ACCACCGATT
CTATGCCCCC
ACTTCTGAGA
CAAGCAATTG
AGCTGTTCGA
CCGGCAGGGA
GCCGAACCTG
CCTTCCTTTT
CGGCCTGGAA
CTAATCATAT
GTGGCCTGGA
GAAACAGCTA
AAGTGCGAAA
GCGGCGGGCC
GACCGACGCC
CTTGACGATT
TTGACTTAGA
CATGCTCCCA
GCCGATGCCC
TTGACGACTT
TGACCTTGAT
ATGCTGCCTG
CTGACGCTCT
TGACGATTTT
GACCTTGACA
TGCTCCCCGG
GTAACTCGAG
CGCGTTAAGT
CGACAATCAA
CCTCTGGATT
ACAAAATTTG
TGAAAGATTG
ACTGGTATTC
TTAACTATGT
TGCTCCTTTT
ACGCTATGTG
GATACGCTGC
TTTAATGCCT
TTGTATCATG
CTATTGCTTC
CCGTATGGCT
TTCATTTTCT
CCTCCTTGTA
TAAATCCTGG
TTGCTGTCTC
TTTATGAGGA
GTTGTGGCCC
GTTGTCAGGC
AACGTGGCGT
GGTGTGCACT
GTGTTTGCTG
ACGCAACCCC
CACTGGTTGG
GGCATTGCCA
CCACCTGTCA
GCTCCTTTCC
GGGACTTTCG
CTTTCCCCCT
CCCTATTGCC
ACGGCGGAAC
TCATCGCCGC
CTGCCTTGCC
CGCTGCTGGA
CAGGGGCTCG
GCTGTTGGGC
ACTGACAATT
CCGTGGTGTT
GTCGGGGAAA
TCATCGTCCT
TTCCTTGGCT
GCTCGCCTGT
GTTGCCACCT
GGATTCTGCG
CGGGACGTCC
TTCTGCTACG
TCCCTTCGGC
CCTCAATCCA
GCGGACCTTC
CTTCCCGCGG
CCTGCTGCCG
GCTCTGCGGC
CTCTTCCGCG
TCTTCGCCTT
CGCCCTCAGA
CGAGTCGGAT
CTCCCTTTGG
GCCGCCTCCC
CGCGTCGACT
TTAAGACCAA
TGACTTACAA
GGCAGCTGTA
GATCTTAGCC
ACTTTTTAAA
AGAAAAGGGG
GGACTGGAAG
GGCTAATTCA
CTCCCAACGA
AGACAAGATC
TGCTTTTTGC
TTGTACTGGG
TCTCTCTGGT
TAGACCAGAT
CTGAGCCTGG
GAGCTCTCTG
GCTAACTAGG
GAACCCACTG
CTTAAGCCTC
AATAAAGCTT
GCCTTGAGTG
CTTCAAGTAG
TGTGTGCCCG
TCTGTTGTGT
GACTCTGGTA
ACTAGAGATC
CCTCAGACCC
TTTTAGTCAG
TGTGGAAAAT
CTCTAGCAGG
GCCCGTTTAA
ACCCGCTGAT
CAGCCTCGAC
TGTGCCTTCT
AGTTGCCAGC
CATCTGTTGT
TTGCCCCTCC
CCCGTGCCTT
CCTTGACCCT
GGAAGGTGCC
ACTCCCACTG
TCCTTTCCTA
ATAAAATGAG
GAAATTGCAT
CGCATTGTCT
GAGTAGGTGT
CATTCTATTC
TGGGGGGTGG
GGTGGGGCAG
GACAGCAAGG
GGGAGGATTG
GGAAGACAAT
AGCAGGCATG
CTGGGGATGC
GGTGGGCTCT
ATGGCTTCTG
AGGCGGAAAG
AACCAGCTGG
GGCTCTAGGG
GGTATCCCCA
CGCGCCCTGT
AGCGGCGCAT
TAAGCGCGGC
GGGTGTGGTG
GTTACGCGCA
GCGTGACCGC
TACACTTGCC
AGCGCCCTAG
CGCCCGCTCC
TTTCGCTTTC
TTCCCTTCCT
TTCTCGCCAC
GTTCGCCGGC
TTTCCCCGTC
AAGCTCTAAA
TCGGGGGCTC
CCTTTAGGGT
TCCGATTTAG
TGCTTTACGG
CACCTCGACC
CCAAAAAACT
TGATTAGGGT
GATGGTTCAC
GTAGTGGGCC
ATCGCCCTGA
TAGACGGTTT
TTCGCCCTTT
GACGTTGGAG
TCCACGTTCT
TTAATAGTGG
ACTCTTGTTC
CAAACTGGAA
CAACACTCAA
CCCTATCTCG
GTCTATTCTT
TTGATTTATA
AGGGATTTTG
CCGATTTCGG
CCTATTGGTT
AAAAAATGAG
CTGATTTAAC
AAAAATTTAA
CGCGAATTAA
TTCTGTGGAA
TGTGTGTCAG
TTAGGGTGTG
GAAAGTCCCC
AGGCTCCCCA
GCAGGCAGAA
GTATGCAAAG
CATGCATCTC
AATTAGTCAG
CAACCAGGTG
TGGAAAGTCC
CCAGGCTCCC
CAGCAGGCAG
AAGTATGCAA
AGCATGCATC
TCAATTAGTC
AGCAACCATA
GTCCCGCCCC
TAACTCCGCC
CATCCCGCCC
CTAACTCCGC
CCAGTTCCGC
CCATTCTCCG
CCCCATGGCT
GACTAATTTT
TTTTATTTAT
GCAGAGGCCG
AGGCCGCCTC
TGCCTCTGAG
CTATTCCAGA
AGTAGTGAGG
AGGCTTTTTT
GGAGGCCTAG
GCTTTTGCAA
AAAGCTCCCG
GGAGCTTGTA
TATCCATTTT
CGGATCTGAT
CAGCACGTGT
TGACAATTAA
TCATCGGCAT
AGTATATCGG
CATAGTATAA
TACGACAAGG
TGAGGAACTA
AACCATGGCC
AAGTTGACCA
GTGCCGTTCC
GGTGCTCACC
GCGCGCGACG
TCGCCGGAGC
GGTCGAGTTC
TGGACCGACC
GGCTCGGGTT
CTCCCGGGAC
TTCGTGGAGG
ACGACTTCGC
CGGTGTGGTC
CGGGACGACG
TGACCCTGTT
CATCAGCGCG
GTCCAGGACC
AGGTGGTGCC
GGACAACACC
CTGGCCTGGG
TGTGGGTGCG
CGGCCTGGAC
GAGCTGTACG
CCGAGTGGTC
GGAGGTCGTG
TCCACGAACT
TCCGGGACGC
CTCCGGGCCG
GCCATGACCG
AGATCGGCGA
GCAGCCGTGG
GGGCGGGAGT
TCGCCCTGCG
CGACCCGGCC
GGCAACTGCG
TGCACTTCGT
GGCCGAGGAG
CAGGACTGAC
ACGTGCTACG
AGATTTCGAT
TCCACCGCCG
CCTTCTATGA
AAGGTTGGGC
TTCGGAATCG
TTTTCCGGGA
CGCCGGCTGG
ATGATCCTCC
AGCGCGGGGA
TCTCATGCTG
GAGTTCTTCG
CCCACCCCAA
CTTGTTTATT
GCAGCTTATA
ATGGTTACAA
ATAAAGCAAT
AGCATCACAA
ATTTCACAAA
TAAAGCATTT
TTTTCACTGC
ATTCTAGTTG
TGGTTTGTCC
AAACTCATCA
ATGTATCTTA
TCATGTCTGT
ATACCGTCGA
CCTCTAGCTA
GAGCTTGGCG
TAATCATGGT
CATAGCTGTT
TCCTGTGTGA
AATTGTTATC
CGCTCACAAT
TCCACACAAC
ATACGAGCCG
GAAGCATAAA
GTGTAAAGCC
TGGGGTGCCT
AATGAGTGAG
CTAACTCACA
TTAATTGCGT
TGCGCTCACT
GCCCGCTTTC
CAGTCGGGAA
ACCTGTCGTG
CCAGCTGCAT
TAATGAATCG
GCCAACGCGC
GGGGAGAGGC
GGTTTGCGTA
TTGGGCGCTC
TTCCGCTTCC
TCGCTCACTG
ACTCGCTGCG
CTCGGTCGTT
CGGCTGCGGC
GAGCGGTATC
AGCTCACTCA
AAGGCGGTAA
TACGGTTATC
CACAGAATCA
GGGGATAACG
CAGGAAAGAA
CATGTGAGCA
AAAGGCCAGC
AAAAGGCCAG
GAACCGTAAA
AAGGCCGCGT
TGCTGGCGTT
TTTCCATAGG
CTCCGCCCCC
CTGACGAGCA
TCACAAAAAT
CGACGCTCAA
GTCAGAGGTG
GCGAAACCCG
ACAGGACTAT
AAAGATACCA
GGCGTTTCCC
CCTGGAAGCT
CCCTCGTGCG
CTCTCCTGTT
CCGACCCTGC
CGCTTACCGG
ATACCTGTCC
GCCTTTCTCC
CTTCGGGAAG
CGTGGCGCTT
TCTCATAGCT
CACGCTGTAG
GTATCTCAGT
TCGGTGTAGG
TCGTTCGCTC
CAAGCTGGGC
TGTGTGCACG
AACCCCCCGT
TCAGCCCGAC
CGCTGCGCCT
TATCCGGTAA
CTATCGTCTT
GAGTCCAACC
CGGTAAGACA
CGACTTATCG
CCACTGGCAG
CAGCCACTGG
TAACAGGATT
AGCAGAGCGA
GGTATGTAGG
CGGTGCTACA
GAGTTCTTGA
AGTGGTGGCC
TAACTACGGC
TACACTAGAA
GAACAGTATT
TGGTATCTGC
GCTCTGCTGA
AGCCAGTTAC
CTTCGGAAAA
AGAGTTGGTA
GCTCTTGATC
CGGCAAACAA
ACCACCGCTG
GTAGCGGTGG
TTTTTTTGTT
TGCAAGCAGC
AGATTACGCG
CAGAAAAAAA
GGATCTCAAG
AAGATCCTTT
GATCTTTTCT
ACGGGGTCTG
ACGCTCAGTG
GAACGAAAAC
TCACGTTAAG
GGATTTTGGT
CATGAGATTA
TCAAAAAGGA
TCTTCACCTA
GATCCTTTTA
AATTAAAAAT
GAAGTTTTAA
ATCAATCTAA
AGTATATATG
AGTAAACTTG
GTCTGACAGT
TACCAATGCT
TAATCAGTGA
GGCACCTATC
TCAGCGATCT
GTCTATTTCG
TTCATCCATA
GTTGCCTGAC
TCCCCGTCGT
GTAGATAACT
ACGATACGGG
AGGGCTTACC
ATCTGGCCCC
AGTGCTGCAA
TGATACCGCG
AGACCCACGC
TCACCGGCTC
CAGATTTATC
AGCAATAAAC
CAGCCAGCCG
GAAGGGCCGA
GCGCAGAAGT
GGTCCTGCAA
CTTTATCCGC
CTCCATCCAG
TCTATTAATT
GTTGCCGGGA
AGCTAGAGTA
AGTAGTTCGC
CAGTTAATAG
TTTGCGCAAC
GTTGTTGCCA
TTGCTACAGG
CATCGTGGTG
TCACGCTCGT
CGTTTGGTAT
GGCTTCATTC
AGCTCCGGTT
CCCAACGATC
AAGGCGAGTT
ACATGATCCC
CCATGTTGTG
CAAAAAAGCG
GTTAGCTCCT
TCGGTCCTCC
GATCGTTGTC
AGAAGTAAGT
TGGCCGCAGT
GTTATCACTC
ATGGTTATGG
CAGCACTGCA
TAATTCTCTT
ACTGTCATGC
CATCCGTAAG
ATGCTTTTCT
GTGACTGGTG
AGTACTCAAC
CAAGTCATTC
TGAGAATAGT
GTATGCGGCG
ACCGAGTTGC
TCTTGCCCGG
CGTCAATACG
GGATAATACC
GCGCCACATA
GCAGAACTTT
AAAAGTGCTC
ATCATTGGAA
AACGTTCTTC
GGGGCGAAAA
CTCTCAAGGA
TCTTACCGCT
GTTGAGATCC
AGTTCGATGT
AACCCACTCG
TGCACCCAAC
TGATCTTCAG
CATCTTTTAC
TTTCACCAGC
GTTTCTGGGT
GAGCAAAAAC
AGGAAGGCAA
AATGCCGCAA
AAAAGGGAAT
AAGGGCGACA
CGGAAATGTT
GAATACTCAT
ACTCTTCCTT
TTTCAATATT
ATTGAAGCAT
TTATCAGGGT
TATTGTCTCA
TGAGCGGATA
CATATTTGAA
TGTATTTAGA
AAAATAAACA
AATAGGGGTT
CCGCGCACAT
TTCCCCGAAA
AGTGCCACCT
GACGTCGACG
GATCGGGAGA
TCTCCCGATC
CCCTATGGTG
CACTCTCAGT
ACAATCTGCT
CTGATGCCGC
ATAGTTAAGC
CAGTATCTGC
TCCCTGCTTG
TGTGTTGGAG
GTCGCTGAGT
AGTGCGCGAG
CAAAATTTAA
GCTACAACAA
GGCAAGGCTT
GACCGACAAT
TGCATGAAGA
ATCTGCTTAG
GGTTAGGCGT
TTTGCGCTGC
TTCGCGATGT
ACGGGCCAGA
TATACGCGTT
GACATTGATT
ATTGACTAGT
TATTAATAGT
AATCAATTAC
GGGGTCATTA
GTTCATAGCC
CATATATGGA
GTTCCGCGTT
ACATAACTTA
CGGTAAATGG
CCCGCCTGGC
TGACCGCCCA
ACGACCCCCG
CCCATTGACG
TCAATAATGA
CGTATGTTCC
CATAGTAACG
CCAATAGGGA
CTTTCCATTG
ACGTCAATGG
GTGGAGTATT
TACGGTAAAC
TGCCCACTTG
GCAGTACATC
AAGTGTATCA
TATGCCAAGT
ACGCCCCCTA
TTGACGTCAA
TGACGGTAAA
TGGCCCGCCT
GGCATTATGC
CCAGTACATG
ACCTTATGGG
ACTTTCCTAC
TTGGCAGTAC
ATCTACGTAT
TAGTCATCGC
TATTACCATG
GTGATGCGGT
TTTGGCAGTA
CATCAATGGG
CGTGGATAGC
GGTTTGACTC
ACGGGGATTT
CCAAGTCTCC
ACCCCATTGA
CGTCAATGGG
AGTTTGTTTT
GGCACCAAAA
TCAACGGGAC
TTTCCAAAAT
GTCGTAACAA
CTCCGCCCCA
TTGACGCAAA
TGGGCGGTAG
GCGTGTACGG
TGGGAGGTCT
ATATAAGCAG
CGCGTTTTGC
CTGTACTGGG
TCTCTCTGGT
TAGACCAGAT
CTGAGCCTGG
GAGCTCTCTG
GCTAACTAGG
GAACCCACTG
CTTAAGCCTC
AATAAAGCTT
GCCTTGAGTG
CTTCAAGTAG
TGTGTGCCCG
TCTGTTGTGT
GACTCTGGTA
ACTAGAGATC
CCTCAGACCC
TTTTAGTCAG
TGTGGAAAAT
CTCTAGCAGT
GGCGCCCGAA
CAGGGACTTG
AAAGCGAAAG
GGAAACCAGA
GGAGCTCTCT
CGACGCAGGA
CTCGGCTTGC
TGAAGCGCGC
ACGGCAAGAG
GCGAGGGGCG
GCGACTGGTG
AGTACGCCAA
AAATTTTGAC
TAGCGGAGGC
TAGAAGGAGA
GAGATGGGTG
CGAGAGCGTC
AGTATTAAGC
GGGGGAGAAT
TAGATCGCGA
TGGGAAAAAA
TTCGGTTAAG
GCCAGGGGGA
AAGAAAAAAT
ATAAATTAAA
ACATATAGTA
TGGGCAAGCA
GGGAGCTAGA
ACGATTCGCA
GTTAATCCTG
GCCTGTTAGA
AACATCAGAA
GGCTGTAGAC
AAATACTGGG
ACAGCTACAA
CCATCCCTTC
AGACAGGATC
AGAAGAACTT
AGATCATTAT
ATAATACAGT
AGCAACCCTC
TATTGTGTGC
ATCAAAGGAT
AGAGATAAAA
GACACCAAGG
AAGCTTTAGA
CAAGATAGAG
GAAGAGCAAA
ACAAAAGTAA
GACCACCGCA
CAGCAAGCGG
CCGCTGATCT
TCAGACCTGG
AGGAGGAGAT
ATGAGGGACA
ATTGGAGAAG
TGAATTATAT
AAATATAAAG
TAGTAAAAAT
TGAACCATTA
GGAGTAGCAC
CCACCAAGGC
AAAGAGAAGA
GTGGTGCAGA
GAGAAAAAAG
AGCAGTGGGA
ATAGGAGCTT
TGTTCCTTGG
GTTCTTGGGA
GCAGCAGGAA
GCACTATGGG
CGCAGCGTCA
ATGACGCTGA
CGGTACAGGC
CAGACAATTA
TTGTCTGGTA
TAGTGCAGCA
GCAGAACAAT
TTGCTGAGGG
CTATTGAGGC
GCAACAGCAT
CTGTTGCAAC
TCACAGTCTG
GGGCATCAAG
CAGCTCCAGG
CAAGAATCCT
GGCTGTGGAA
AGATACCTAA
AGGATCAACA
GCTCCTGGGG
ATTTGGGGTT
GCTCTGGAAA
ACTCATTTGC
ACCACTGCTG
TGCCTTGGAA
TGCTAGTTGG
AGTAATAAAT
CTCTGGAACA
GATTTGGAAT
CACACGACCT
GGATGGAGTG
GGACAGAGAA
ATTAACAATT
ACACAAGCTT
AATACACTCC
TTAATTGAAG
AATCGCAAAA
CCAGCAAGAA
AAGAATGAAC
AAGAATTATT
GGAATTAGAT
AAATGGGCAA
GTTTGTGGAA
TTGGTTTAAC
ATAACAAATT
GGCTGTGGTA
TATAAAATTA
TTCATAATGA
TAGTAGGAGG
CTTGGTAGGT
TTAAGAATAG
TTTTTGCTGT
ACTTTCTATA
GTGAATAGAG
TTAGGCAGGG
ATATTCACCA
TTATCGTTTC
AGACCCACCT
CCCAACCCCG
AGGGGACCCG
ACAGGCCCGA
AGGAATAGAA
GAAGAAGGTG
GAGAGAGAGA
CAGAGACAGA
TCCATTCGAT
TAGTGAACGG
ATCGGCACTG
CGTGCGCCAA
TTCTGCAGAC
AAATGGCAGT
ATTCATCCAC
AATTTTAAAA
GAAAAGGGGG
GATTGGGGGG
TACAGTGCAG
GGGAAAGAAT
AGTAGACATA
ATAGCAACAG
ACATACAAAC
TAAAGAATTA
CAAAAACAAA
TTACAAAAAT
TCAAAATTTT
CGGGTTTATT
ACAGGGACAG
CAGAGATCCA
GTTTGGTTAA
TTAAGGTACC
GAGGGCCTAT
TTCCCATGAT
TCCTTCATAT
TTGCATATAC
GATACAAGGC
TGTTAGAGAG
ATAATTAGAA
TTAATTTGAC
TGTAAACACA
AAGATATTAG
TACAAAATAC
GTGACGTAGA
AAGTAATAAT
TTCTTGGGTA
GTTTGCAGTT
TTAAAATTAT
GTTTTAAAAT
GGACTATCAT
ATGCTTACCG
TAACTTGAAA
GTATTTCGAT
TTCTTGGCTT
TATATATCTT
GTGGAAAGGA
CGAAACACCG
TAAGCCAGAA
CAGACTCGGC
GTTTTAGAGC
TAGAAATAGC
AAGTTAAAAT
AAGGCTAGTC
CGTTATCAAC
TTGAAAAAGT
GGCACCGAGT
CGGTGCTTTT
TTGAATTCAC
TTTGGCCGCG
AATCGATATG
TCGAGTTTAC
TCCCTATCAG
TGATAGAGAA
CGT