名称: | pQCasTns(Ptr)-IS186 |
---|---|
别称: | Plasmid#175581 |
基因长: | |
质粒宿主: | 大肠杆菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR,转座 |
片段物种: | |
原核抗性: | Kan |
筛选标记: | |
荧光标记: | |
启动子: | |
复制子: | ColA |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | Tet-R |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AACCTTCGAT
CAAACCGCCT
CCCCAGGCGG
TTTTTTCGTT
TACAGAGCAG
GAGATTACGA
CGATCGTAAA
AGGATCTCAA
GAAGATCCTT
TACGGATTCC
CGACACCATC
ACTCTAGATT
TCAGTGCAAT
TTATCTCTTC
AAATGTAGCA
CCTGAAGTCA
GCCCCATACG
ATATAAGTTG
TAATTCTCAT
GTTAGTCATG
CCCCGCGCCC
ACCGGAAGGA
GCTGACTGGG
TTGAAGGCTC
TCAAGGGCAT
CGGTCGAGAT
CCCGGTGCCT
AATGAGTGAG
CTAACTTACA
TTAATTGCGT
TGCGCTGATG
AATCCCCTAA
TGATTTTTAT
CAAAATCATT
AAGGTTACCA
TCACGGAAAA
AGGTTATGCT
GCTTTTAAGA
CCCACTTTCA
CATTTAAGTT
GTTTTTCTAA
TCCGCATATG
ATCAATTCAA
GGCCGAATAA
GAAGGCTGGC
TCTGCACCTT
GGTGATCAAA
TAATTCGATA
GCTTGTCGTA
ATAATGGCGG
CATACTATCA
GTAGTAGGTG
TTTCCCTTTC
TTCTTTAGCG
ACTTGATGCT
CTTGATCTTC
CAATACGCAA
CCTAAAGTAA
AATGCCCCAC
AGCGCTGAGT
GCATATAATG
CATTCTCTAG
TGAAAAACCT
TGTTGGCATA
AAAAGGCTAA
TTGATTTTCG
AGAGTTTCAT
ACTGTTTTTC
TGTAGGCCGT
GTACCTAAAT
GTACTTTTGC
TCCATCGCGA
TGACTTAGTA
AAGCACATCT
AAAACTTTTA
GCGTTATTAC
GTAAAAAATC
TTGCCAGCTT
TCCCCTTCTA
AAGGGCAAAA
GTGAGTATGG
TGCCTATCTA
ACATCTCAAT
GGCTAAGGCG
TCGAGCAAAG
CCCGCTTATT
TTTTACATGC
CAATACAATG
TAGGCTGCTC
TACACCTAGC
TTCTGGGCGA
GTTTACGGGT
TGTTAAACCT
TCGATTCCGA
CCTCATTAAG
CAGCTCTAAT
GCGCTGTTAA
TCACTTTACT
TTTATCTAAT
CTAGACATCA
TTAATTCCTA
ATTTTTGTTG
ACACTCTATC
ATTGATAGAG
TTATTTTACC
ACTCCCTATC
AGTGATAGAG
AAAAGTGAAC
TCTAGAAATA
ATTTTGTTTA
ACTTTAAAAG
GAGATATACC
ATGGGTGAAC
TGCCGAGTAG
GCAGCTGGAA
ATTGGATGCT
TTGCGTGCAA
AGGAACCTGA
ACTCGTGAAC
TGCCGAGTAG
GCAGCTGAAG
TTGGATCCGA
ATTCGAGCTC
GGGAAGAAGG
AGATATACAT
ATGCACTTTC
TGGTGCAGAC
CAAGAGCTAC
CCGGACGAGG
CGCTGGAAAG
CTATCTGCTG
CGTCTGGCGC
GTGATAACAG
CTACAACGGT
TATAGCGAGC
TGGCGGACAT
CCTGTGGCAG
TGGCTGGCGG
AACAAGATAA
CGAGCTGGAA
GGTGCGCTGC
CGCTGGCGCT
GAGCAAGGTG
GACGTTTACC
ACGCGCGTCA
GGCGAGCAGC
TTCCGTATCC
GTGCGCTGAA
ACTGGTGGCG
CAACTGGCGG
ACGTTAACGC
GGGTGATATT
CTGGCGCTGG
CGTGGCGTCG
TAGCAACTTC
AAGTTTGGCA
ACCTGGCGGC
GGTGAGCCGT
AACGAGCTGG
CGATCCCGCT
GGAACTGCTG
CGTACCGATA
ACATCCCGGT
TTGCATTAAA
TGCCTGAGCG
AGAGCAGCCA
CATTCCGTTT
TACTGGCACC
TGAAGCCGTA
TAAAGCGTGC
CACAAGCACA
AAAGCCAGCT
GATCACCCGT
TGCAAGGAGT
GCTACGACCT
GATTGATTAT
CGTGCGAGCG
AGGCGTTTCT
GGAATGCGTT
TGCGGTTGCA
AAATCACCAA
CAGCGAACAA
CTGAACGACG
CGGATTTCAA
GATCGCGATT
GCGCTGGCGA
GCAGCAACAG
CCAGAAAATC
GTGGGCCTGA
TTAGCTGGTT
CGCGAAGGTG
AAACAACTGG
ACGTTAGCGA
CGCGGATTTC
AACTGCGCGT
TTGTTGATTA
CTTCAACACC
TGGCCGGAGA
GCCTGACCAC
CGAACTGGAC
CTGCTGACCA
ACAACGCGCG
TCTGAAGCAG
CTGAACCCGT
TTAACAAGAC
CAAATTCAGC
AGCGTGTACG
GTGACCTGAT
CCGTGATGGC
CAAATTGCGG
CGACCAGCAA
CCGTAAGAAC
AAAGTTATCG
ACGAGATCAT
TAGCTATTTT
GTGGAACTGG
TTGATAGCAA
CCCGAAGGCG
AAACACCCGA
ACATTGGTGA
CCTGCTGCTG
TGCACCTTCG
ATGCGGCGGT
GCTGCTGAAC
ACCACCACCG
AGCAGGTTTA
CCGTCTGCAC
CAAGAAGCGT
TTCTGAACTG
CGCGTATAGC
CAGAAGAAAC
ACGAACAACT
GCGTGCGGAT
AGCCACGTGT
TCTATCTGCG
TCAGGTTATC
GAGCTGCAGC
AAGCGTTTGC
GGCGGAAAAA
CCGCTGACCA
AGAAACAATT
CATTGCGCCG
TGGTAACTTA
TGAACCTGCA
GGATGCGCTG
GCGATTGAGC
CGCTGAAGGA
AAAAACCACC
GCGCTGCGTA
AGCTGTTCGT
GCCGTACACC
AGCCACGTTG
AGGTGGATGG
TTTTGAGGAA
CTGGCGCTGA
CCGTGCTGAT
CAACCTGGTT
TATAAGCGTA
GCGAAATTGA
CGATCTGACC
AGCGCGCGTA
CCGCGAAAAG
CGTGCTGCGT
GACGAGGTTC
TGCTGAGCAA
GTGCATCAAC
GAAGTGAAAT
GGTTCCACAC
CCACAACCTG
AAGTACCCGG
ACATCCGTGT
TAGCCACCAA
CGTCTGATTA
GCGAGGTGGT
TAGCGAAGAT
ATCGCGGGTA
TTTGCAGCCG
TAGCCTGCCG
CTGAGCTTTG
GCTGGAGCCA
CAACAGCGCG
GAGATCAACC
ACGCGAAACT
GTTCCTGACC
AGCTTTAACT
GGCAGGGTGA
AGTGACCTGC
CTGGCGCGTC
TGCTGATTAA
CGAGGAACCG
GTTTGGATCA
ACCTGATTCG
TGCGTACGGT
TTCACCAAGA
AAGCGGTTCT
GGAGATCAGC
GGCAAGATTA
AACAGCAACT
GCCGGTGGCG
GAGTTCCCGC
TGGAAGTTAG
CAGCTTTAGC
CCGCAGCTGC
AAATGCCGTT
TCAGCAAAGC
TATCTGGTGG
TTACCCCGGT
GGTTAGCCAC
GCGATGCTGG
CGAAGATCCA
GCAACTGACC
ACCGACCGTA
AACTGAACTT
CGCGCTGGTT
GAGCACAGCC
GTCCGGCGAA
CGTTGGTGAT
CTGGCGAGCA
GCGTGGGTGG
CAACATTCGT
GTTCTGCGTT
ACTTTCCGAA
GACCTATAGC
AAAGCGGTGA
ACCGTAGCAA
AGTTGCGAAC
AACGATATCG
AAAAGGCGTT
CAAAATTCGT
GCGCTGCTGA
GCAGCCAGTT
TCAGCAAGCG
CTGCTGGTGC
TGGTTGGCAT
CAAGCAGTTC
AACACCCTGC
GTCAAAAACG
TCTGGCGCGT
GTGGCGGCGA
TCCGTCAAGT
GCGTGTTAGC
CTGCAACTGT
GGCTGGACAA
CATTCTGGAG
GCGAAGAACA
ACGCGCAGAA
CCAAGTGTAC
CCGGAATGGG
TTCGTCACTA
TCTGGATCAA
AGCATCACCA
ACTGCATTAG
CCAGTTCAGC
AACGTTCTGA
ACGAAAGCCT
GGGTAACCTG
AGCAAGCTGA
AACGTTTTGC
GTACCACCCG
AACCTGATGG
GCCTGTTCAA
AGCGCAACTG
AACTATGTGT
TTACCCACTG
CGCGGCGGAG
CAGGAAATCC
TGAACGACGA
GCAAATTGTG
TACGTTCACT
GCCAGGACAT
GCGTGTTTTC
GATGCGGAAG
CGATGGCGAA
CCCGTATATC
CAGGGTATGC
CGAGCCTGAC
CGCGCTGAAC
GGCCTGGCGC
ACAACTTCGA
GCGTAAGCTG
AAAAACTTTA
TTGATCCGAG
CATCAAGTGC
ATTGGTAGCG
CGATCTACAT
TGAGAACTAT
CAACTGCACA
CCGGCAAACC
GCTGCCGGAA
CCGAGCAAGC
TGAAACAGGT
GGCGGGTCGT
AGCCACGTTA
TCCGTAGCGG
CATCATTGAC
AAGCCGAAAT
GCGACATTAC
CCTGGATCTG
GTGTTCCGTC
TGTTTGTTCC
GAACACCGAA
CTGCTGGATA
AGCTGAACAG
CCAACTGATT
AAGCCGGCGC
TGCCGAGCAG
CTTTGCGGGT
GGCACCATGC
ACCCGCCGAG
CCTGTACCAG
AACATTGACT
GGTGCCACGT
GCACACCAAG
CCGAGCGAGC
TGTTTAAGAA
ACTGAAGGCG
AAAAGCAGCA
ACGGTAGCTG
GCTGTATCCG
AGCAAGAAAG
TGGTTAAAAG
CTTCGAACAG
CTGATCGACG
CGCTGAACAG
CAACTTTAAC
CTGCGTCCGG
CGGCGATTGG
CCTGGCGGCG
CTGGAGGAAC
CGGTGAAGCG
TGATGCGGCG
CTGCACGAGT
ACCACTGCTA
TGCGGAACCG
GTTATCGGTC
TGCTGGAGTG
CGTGAGCAAC
ACCAGCGTTA
AGTACGCGGG
CGCGAAACAA
TTCTTTCACG
ACGCGTTCTG
GGTGATGGAT
GTTCAGAAGG
AAAGCATGCT
GATGAAGAAA
AGCAAATTTG
AGTATGAATA
ATGCAGCTGC
CGCGTCACCT
GAGCTACACC
CGTAGCCTGA
GCCCGAGCAA
GGCGGTGTTC
TTTTATAAAA
CCCCGGAGAG
CGACTTCGAA
CCGCTGCAGA
TCGAGCAAAA
CAAACTGGTG
GGTCAGAAGA
GCGGTTTTGG
CGATGCGTAC
CAGAAGCAAA
ACGTTGCGAA
AAACCTGGCG
CCGCAGGACC
TGGCGTTTGG
TAACCCGCAA
ACCATTGATG
TGTGCTATGT
TCCGCCGACC
GTGAACGAAC
TGTTCTGCCG
TTTTAGCCTG
CGTGTTGAGG
CGAACTGCAT
CGAACCGCAC
GTGTGCGACG
ATCCGAAGGT
TATTTACTGG
CTGAAACGTT
TCTTTGAAAC
CTATAAGAAA
CACAACGGTC
TGAACGAAGT
GGCGACCCGT
TACGCGAAGA
ACATCCTGAT
GGGCAACTGG
CTGTGGCGTA
ACCGTCAGAG
CCCGAACGTT
GACATCGAGA
TTCTGACCGA
ACACGCGGCG
CCGATTGTGG
TTGAGGGTGC
GCAGAAGCTG
AAATGGCAAG
GCAACTGGCA
GAACAACCAA
ACCGCGCTGC
TGACCCTGAG
CGAGAGCATC
CAGGAAGGTC
TGAGCAACCC
GCAAAACTAC
TGCTATCTGG
ATATCACCGC
GAAGATTAAA
AACGCGTTCA
GCCAGGAAGT
GCACCCGAGC
CAAAAGTTTG
TGGACAACGT
TGAACAGGGT
ATGAGCAGCA
AACAGCTGGC
GTATACCCAA
GTGGGCGATA
AGAAAGCGGC
GAGCCTGAAC
AGCCAGAAGG
TTGGCGCGGC
GATCCAAACC
ATTGACGATT
GGTACGAGGA
AGGTTATAAA
CCGCTGCGTA
CCCATGAGTA
TGGTGCGGAC
AAGCAAATCC
TGGTGGCGCA
CCGTACCCCG
AAAAGCCACA
GCGATTTTTA
TAGCCTGCTG
CCGCGTATCG
CGCTGCACAT
TAAGCACATG
GAAAAACACG
GTCTGGAGCA
GAGCGAACAA
AGCAACAGCA
TCCACTTCAT
TGCGGCGGTT
CTGATTAAGG
GTGGCCTGTT
TCAGCGTAGC
AAAGGATGAA
GCGTTACTAT
TTCACCATCA
CCTACCTGCC
GCAAAGCTGC
GATGTGAGCC
TGCTGGCGGG
TCGTTGCATC
GGCATTCTGC
ACGGTTTCAT
GAGCAGCCGT
GAGATCAGCA
ACATTGGCGT
GTGCTTTCCG
AAATGGAACG
AGCAGACCAT
CGGTAACGAA
CTGGCGTTTG
TTAGCACCAA
CAAGAAACAA
CTGACCAACC
TGAGCCAGCA
AAGCTATTTC
GAGATGATGG
CGCACGACAA
GCTGTTTGGC
CTGAGCAAAA
TTCTGGAAGT
GCCGGTTAAC
CAGAGCGAAG
TGATGTTCGT
TCGTAACCAA
AGCGTGGCGA
AGGCGTTTGT
TGGTGAAAAG
CAACGTCGTC
TGAAACGTGC
GAAGAAACGT
GCGGAGGCGC
GTGGCGAAGT
GTACAACCCG
GAGTATAAGT
TCGAAGCGAA
AGATATCGGT
CACTTTCACA
GCATTCCGGT
GAGCAGCAAG
GGTAACGGCC
AGAGCTACGT
TCTGCACATC
CAAAAGAACG
AGAACGCGGA
AAGCATTAAA
AACCAGTTCA
ACAACTATGG
CTTTGCGACC
AACCAAATTT
TCCTGGGCAC
CGTGCCGAGC
CTGAACACCC
TGCTGTAAAA
GGAGATATAC
CATGTACCGT
CGTAAGCTGA
AATATAGCCG
TGTTAAGAAC
CTGCACAAAT
TTGCGAGCCA
GAAGAACAAA
AGCACCTGCC
TGGTGGAGAG
CAGCCTGGAA
TTCGACGCGT
GCTTCCACTT
TGAGTTCAGC
CCGCCGATCG
CGGCGTTTGA
AGCGCAACCG
CTGGGTTACG
AGTATGAATT
CGATAACCGT
ATTTGCCGTT
ACACCCCGGA
CTTTCTGCTG
ACCCACACCG
ATGGCACCCA
GAAGTTCATC
GAGGTTAAGC
CGCAAAGCAA
AATTGCGGAC
GAGGATTTTC
GTGCGCGTTT
CATCGAAAAG
CAGGCGATTG
CGAAACAAGA
CGGTCGTGAT
CTGATCCTGG
TGACCGACAA
GCAGATTCGT
GTTTACCCGA
CCCTGAACAA
CCTGAAACTG
CTGCACCGTT
ATAGCGGCTT
TCAGAGCCTG
ACCGAGCTGC
AAGCGAGCGT
GCTGGAACTG
GTTAAGCAGT
ACGGTAGCAT
CAAAGTGGGC
CAACTGATTC
GTTATCTGAA
AGTTACCGCG
GGTGAACTGC
TGGCGACCGT
GCTGCGTCTG
CTGAGCCTGG
GCCAACTGTT
CGCGGATCTG
ACCACCAACG
AGATCAGCAT
TGAAACCGCG
ATCTGGAGCA
ACAATGTTTA
ATAACGACCT
GTTCGACGAT
GAGTTTAACC
AGCCGCTGCC
GAAGGCGGAA
ACCAAACTGC
CGCAGAACTA
TACCAAGGAT
CTGCAAGCGC
TGCCGGAGAA
GATCAAAACC
ACCACCTTCG
CGAAGCTGAA
ATACATTCAA
TGGCTGGAGG
CGAACATCCA
GGGTGGCTGG
ACCCAAAAGA
ACCTGGAACC
GCTGCTGAAA
CTGATGCCGG
ACGTTGAGGG
TGAAAAGAAA
CCGAGCTGGC
GTACCGCGGC
GCGTTGGTAT
AGCGCGTACA
CCAACGCGGA
TAAGAACATT
ATGGCGCTGA
TCCCGAGCCA
CCAGAAGAAA
GGCAACCGTG
AACGTGACAC
CACCACCGAT
AAGTTCTTTG
AGAAAGCGCT
GGAACGTTAC
CTGGTGAAGG
AGAAACCGAG
CGTTGCGAGC
GCGTATAAGT
TCTACAAAGA
CCTGGTGATC
ATTGAAAACG
ACAGCGTGGT
TGATAGCGTT
CTGAAACCGC
TGACCTATAA
GGCGTTTAAA
AACCGTATTG
ACAACCTGCC
GCAGTATGAG
GTTATGATCG
CGCGTTACGG
CAAGCGTCTG
GCGGATATTG
CGTACAACAA
GGTGGAAGGC
CACAAACGTC
CGATTCGTGT
GCTGGAGAAA
GTTGAAATCG
ACCACACCCC
GCTGGATCTG
ATTCTGCTGG
ACGATGAGCT
GCACATCCCG
CTGGGTCGTC
CGACCCTGAC
CATGCTGGTT
GACGTTTATA
GCCACTGCAT
CGTGGGCTAC
TATTTCAGCT
TTAGCGAGCC
GAGCTACGAT
GCGGTTCGTC
GTGCGATGCT
GAACGCGATG
AAGCCGAAAA
GCGAAGTGGC
GAAACTGTAC
CCGGACACCA
TTAACGAGTG
GAAGTGCGCG
GGTAAAATCG
AAACCCTGGT
GGTTGATAAC
GGCGCGGAGT
TCTGGAGCAA
CAGCCTGGAA
CTGGCGTGCG
AGGAAATCGG
TATTAACACC
CAGTATAACC
CGGTGGCGAA
GCCGTGGCTG
AAACCGTTCG
TTGAGCGTAT
GTTTGGCACC
ATCAACACCG
AACTGCTGGA
CCCGGTTCCG
GGCAAGACCT
TCAGCAACAT
CCTGCAAAAA
CACGAATACA
ACCCGAAGAA
AGACGCGATT
ATGCGTTTCA
CCACCTTTAT
GCAGCTGTTT
CACAAGTGGG
TGGTTGATGT
GTATCACCAA
GACGCGGATA
GCCGTTTCAA
ATACATTCCG
AGCCAGCTGT
GGGACCAAGG
CTTTAACACC
CTGCCGCCGA
CCATGCTGAG
CGATGCGGAT
CTGCAGCAAC
TGGATGTGGT
TCTGAGCATC
AGCAACCACC
GTGTGCTGCG
TAAGGGTGGC
ATTCGTCTGG
AGAACCTGAG
CTATGACAGC
ACCGAACTGG
CGAACTACCG
TAAGCAGTTC
AGCCACAAAG
TGAGCCAAGA
GGTTCTGATC
AAACTGAACC
CGGACGATAT
TAGCTACATC
TATGTGTACC
TGGACAAGCT
GGAACACTAT
ATTAAAGTTC
CGTGCATCGA
TCCGAACGGT
TACACCCAGA
ACCTGAGCCT
GAACCAACAC
AAGATCAACA
TTCGTATCCA
CCGTGACTTT
ATTAGCGGTA
GCATCGATAA
CGTTGGCCTG
GCGAAGGCGC
GTATGTTCAT
TCACAACAAA
ATCCAGAACG
AGTTTGAGGA
ACTGAAGAAC
GCGCCGAAAC
ACAGCAAGGT
GAAAGGTGGC
AAGGCGCTGG
CGAAACACCA
GAACATTAGC
AGCGACAGCC
AAAAGAGCAT
CACCCACAGC
AAACCGGTGG
AGGCGAAGAA
AGTTACCCCG
AAAGAACAAC
CGACCGATAG
CTGGGACGAT
TTCATCAGCG
ACCTGGATGG
TTTTTAATTA
TGCTGACCGA
CAAGCAGAAA
GAAAAGCTGA
ACGAGTTCCG
TGATGTTTTT
ATTGAATACC
CGATCATTAC
CACCATCTTC
AACGACTTTG
ATCGTCTGCG
TCTGGGTAAA
GGCCTGACCG
GCGAGAAGCC
GTGCATGCTG
CTGAACGGTG
ACACCGGCAC
CGGTAAAACC
GCGCTGATTA
AACAGTATAA
GGAACGTCAC
CTGCCGCAAT
TCATCAACGG
TGTTATGAAC
CACCCGGTGC
TGGTTAGCCG
TATTCCGAGC
AACCCGACCC
TGGAAAGCAC
CCTGGCGGAG
CTGCTGAAAG
ACCTGGGTCA
AGTGGGCAGC
ACCGAGCGTA
AGCTGCGTAT
TAACGGCACC
CGTCTGACCA
CCAGCCTGAT
CAAATGCCTG
AAGACCTGCG
GCACCGAACT
GATCATTATC
GATGAGTTTC
AGGAACTGAT
TGAGCACAAC
CAAGGCAAGA
AACGTCGTGA
AATTGCGAAC
CGTCTGAAAT
ACATCAACGA
CGAGGCGGGT
GTTAGCATTG
TGCTGGTTGG
CATGCCGTGG
GCGGAAAAGA
TCGCGGATGA
GCCGCAGTGG
AGCAGCCGTC
TGCTGATCCG
TCGTCAACTG
CCGTATTTCA
AACTGAGCGA
GAACCCGAAG
CACTTTGTGC
AGCTGATTAT
CGGTCTGGCG
AACCGTATGC
CGTTCGCGGA
AAAACCGAAC
CTGAGCGAGC
AAGCGACCGT
TTTCACCCTG
TTTAGCCTGA
GCAAAGGCTG
CTTCCGTACC
CTGAAGTACT
TTCTGGACGA
TGCGGTGCTG
TATGCGCTGA
TGGACAACGC
GAAGACCCTG
ACCACCAAAC
ACCTGGTGAA
GGCGTTCGAA
GTTCTGTTTC
CGGATGTGCC
GAACCTGTTT
ACCCTGCCGG
TTGCGGAGAT
CACCGCGAGC
GAGGTGGAAC
GTTACAGCCT
GTATAAGCCG
GAAAGCAGCC
AGGACGAGGA
CCCGTTCATT
GCGACCAAAT
TTACCGATCG
TATGCCGATC
AGCCAACTGC
TGCGTAAGTA
ACTCGAGCCG
CTGAGCAATA
ACTAGCATAA
CCCCTTGGGG
CCTCTAAACG
GGTCTTGAGG
GGTTTTTTGC
TGAAACCTCA
GGCATTTGAG
AAGCACACGG
TCACACTGCT
TCCGGTAGTC
AATAAACCGG
TAAACCAGCA
ATAGACATAA
GCGGCTATTT
AACGACCCTG
CCCTGAACCG
ACGACAAGCT
GACGACCGGG
TCTCCGCAAG
TGGCACTTTT
CGGGGAAATG
TGCGCGGAAC
CCCTATTTGT
TTATTTTTCT
AAATACATTC
AAATATGTAT
CCGCTCATGA
ATTAATTCTT
AGAAAAACTC
ATCGAGCATC
AAATGAAACT
GCAATTTATT
CATATCAGGA
TTATCAATAC
CATATTTTTG
AAAAAGCCGT
TTCTGTAATG
AAGGAGAAAA
CTCACCGAGG
CAGTTCCATA
GGATGGCAAG
ATCCTGGTAT
CGGTCTGCGA
TTCCGACTCG
TCCAACATCA
ATACAACCTA
TTAATTTCCC
CTCGTCAAAA
ATAAGGTTAT
CAAGTGAGAA
ATCACCATGA
GTGACGACTG
AATCCGGTGA
GAATGGCAAA
AGTTTATGCA
TTTCTTTCCA
GACTTGTTCA
ACAGGCCAGC
CATTACGCTC
GTCATCAAAA
TCACTCGCAT
CAACCAAACC
GTTATTCATT
CGTGATTGCG
CCTGAGCGAG
ACGAAATACG
CGGTCGCTGT
TAAAAGGACA
ATTACAAACA
GGAATCGAAT
GCAACCGGCG
CAGGAACACT
GCCAGCGCAT
CAACAATATT
TTCACCTGAA
TCAGGATATT
CTTCTAATAC
CTGGAATGCT
GTTTTCCCGG
GGATCGCAGT
GGTGAGTAAC
CATGCATCAT
CAGGAGTACG
GATAAAATGC
TTGATGGTCG
GAAGAGGCAT
AAATTCCGTC
AGCCAGTTTA
GTCTGACCAT
CTCATCTGTA
ACATCATTGG
CAACGCTACC
TTTGCCATGT
TTCAGAAACA
ACTCTGGCGC
ATCGGGCTTC
CCATACAATC
GATAGATTGT
CGCACCTGAT
TGCCCGACAT
TATCGCGAGC
CCATTTATAC
CCATATAAAT
CAGCATCCAT
GTTGGAATTT
AATCGCGGCC
TAGAGCAAGA
CGTTTCCCGT
TGAATATGGC
TCATACTCTT
CCTTTTTCAA
TATTATTGAA
GCATTTATCA
GGGTTATTGT
CTCATGAGCG
GATACATATT
TGAATGTATT
TAGAAAAATA
AACAAATAGG
CATGCTAGCG
CAGAAACGTC
CTAGAAGATG
CCAGGAGGAT
ACTTAGCAGA
GAGACAATAA
GGCCGGAGCG
AAGCCGTTTT
TCCATAGGCT
CCGCCCCCCT
GACGAACATC
ACGAAATCTG
ACGCTCAAAT
CAGTGGTGGC
GAAACCCGAC
AGGACTATAA
AGATACCAGG
CGTTTCCCCC
TGATGGCTCC
CTCTTGCGCT
CTCCTGTTCC
CGTCCTGCGG
CGTCCGTGTT
GTGGTGGAGG
CTTTACCCAA
ATCACCACGT
CCCGTTCCGT
GTAGACAGTT
CGCTCCAAGC
TGGGCTGTGT
GCAAGAACCC
CCCGTTCAGC
CCGACTGCTG
CGCCTTATCC
GGTAACTATC
ATCTTGAGTC
CAACCCGGAA
AGACACGACA
AAACGCCACT
GGCAGCAGCC
ATTGGTAACT
GAGAATTAGT
GGATTTAGAT
ATCGAGAGTC
TTGAAGTGGT
GGCCTAACAG
AGGCTACACT
GAAAGGACAG
TATTTGGTAT
CTGCGCTCCA
CTAAAGCCAG
TTACCAGGTT
AAGCAGTTCC
CCAACTGACT
T