名称: | pWS172 |
---|---|
别称: | Plasmid#90519 |
基因长: | |
质粒宿主: | 酵母菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Kan |
筛选标记: | HIS3 |
荧光标记: | 绿色 |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | Stable |
温度: | 37度 |
背景载体: | |
正向引物: | rrnB-R1 |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TTGAGATCCT
TTTTTTCTGC
GCGTAATCTG
CTGCTTGCAA
ACAAAAAAAC
CACCGCTACC
AGCGGTGGTT
TGTTTGCCGG
ATCAAGAGCT
ACCAACTCTT
TTTCCGAAGG
TAACTGGCTT
CAGCAGAGCG
CAGATACCAA
ATACTGTTCT
TCTAGTGTAG
CCGTAGTTAG
GCCACCACTT
CAAGAACTCT
GTAGCACCGC
CTACATACCT
CGCTCTGCTA
ATCCTGTTAC
CAGTGGCTGC
TGCCAGTGGC
GATAAGTCGT
GTCTTACCGG
GTTGGACTCA
AGACGATAGT
TACCGGATAA
GGCGCAGCGG
TCGGGCTGAA
CGGGGGGTTC
GTGCACACAG
CCCAGCTTGG
AGCGAACGAC
CTACACCGAA
CTGAGATACC
TACAGCGTGA
GCTATGAGAA
AGCGCCACGC
TTCCCGAAGG
GAGAAAGGCG
GACAGGTATC
CGGTAAGCGG
CAGGGTCGGA
ACAGGAGAGC
GCACGAGGGA
GCTTCCAGGG
GGAAACGCCT
GGTATCTTTA
TAGTCCTGTC
GGGTTTCGCC
ACCTCTGACT
TGAGCGTCGA
TTTTTGTGAT
GCTCGTCAGG
GGGGCGGAGC
CTATGGAAAA
ACGCCAGCAA
CGCGGCCTTT
TTACGGTTCC
TGGCCTTTTG
CTGGCCTTTT
GCTCACATGT
TCTTTCCTGC
GTTATCCCCT
GATTCTGTGG
ATAACCGTGC
GGCCGCCCCT
GAATTCGCAT
CTAGACTGAA
CTGGCCGATA
ATTGCAGACG
AACGGTGAGT
AAGGAAAGAG
TGAGGAACTA
TCGCATACCT
GCATTTAAAG
ATGCCGATTT
GGGCGCGAAT
CCTTTATTTT
GGCTTCACCC
TCATACTATT
ATCAGGGCCA
GAAAAAGGAA
GTGTTTCCCT
CCTTCTTGAA
TTGATGTTAC
CCTCATAAAG
CACGTGGCCT
CTTATCGAGA
AAGAAATTAC
CGTCGCTCGT
GATTTGTTTG
CAAAAAGAAC
AAAACTGAAA
AAACCCAGAC
ACGCTCGACT
TCCTGTCATC
CTATTGATTG
CAGCTTCCAA
TTTCGTCACA
CAACAAGGTC
CTAGCGACGG
CTCACAGGTT
TTGTAACAAG
CAATCGAAGG
TTCTGGAATG
GCGGGAAAGG
GTTTAGTACC
ACATGCTATG
ATGCCCACTG
TGATCTCCAG
AGCAAAGTTC
GTTCGATCGT
ACTGTTACTC
TCTCTCTTTC
AAACAGAATT
GTCCGAATCG
TGTGACAACA
ACAGCCTGTT
CTCACACACT
CTTTTCTTCT
AACCAAGGGG
GTGGTTTAGT
TTAGTAGAAC
CTCGTGAAAC
TTACATTTAC
ATATATATAA
ACTTGCATAA
ATTGGTCAAT
GCAAGAAATA
CATATTTGGT
CTTTTCTAAT
TCGTAGTTTT
TCAAGTTCTT
AGATGCTTTC
TTTTTCTCTT
TTTTACAGAT
CATCAAGGAA
GTAATTATCT
ACTTTTTACA
ACAAATATAA
AACAAGATCT
ATGGACAAGA
AGTATTCTAT
CGGACTGGAT
ATCGGGACTA
ATAGCGTCGG
GTGGGCCGTG
ATCACTGACG
AGTACAAGGT
GCCCTCTAAG
AAGTTCAAGG
TGCTCGGGAA
CACCGACCGG
CATTCCATCA
AGAAAAATCT
GATCGGAGCT
CTCCTCTTTG
ATTCAGGGGA
AACCGCTGAA
GCAACCCGCC
TCAAGCGGAC
TGCTAGACGG
CGGTACACCA
GGAGGAAGAA
CCGGATTTGT
TACCTTCAAG
AGATATTCTC
CAACGAAATG
GCAAAGGTCG
ACGACAGCTT
CTTCCATAGG
CTGGAAGAAT
CATTCCTCGT
GGAAGAGGAT
AAGAAGCATG
AACGGCATCC
CATCTTCGGT
AATATCGTCG
ACGAGGTGGC
CTATCACGAG
AAATACCCAA
CCATCTACCA
TCTTCGCAAA
AAGCTGGTGG
ACTCAACCGA
CAAGGCAGAC
CTCCGGCTTA
TCTACCTGGC
CCTGGCCCAC
ATGATCAAGT
TCAGAGGCCA
CTTCCTGATC
GAGGGCGACC
TCAATCCTGA
CAATAGCGAT
GTGGATAAAC
TGTTCATCCA
GCTGGTGCAG
ACTTACAACC
AGCTCTTTGA
AGAGAACCCC
ATCAATGCAA
GCGGAGTCGA
TGCCAAGGCC
ATTCTGTCAG
CCCGGCTGTC
AAAGAGCCGC
AGACTTGAGA
ATCTTATCGC
TCAGCTGCCG
GGTGAAAAGA
AAAATGGACT
GTTCGGGAAC
CTGATTGCTC
TTTCACTTGG
GCTGACTCCC
AATTTCAAGT
CTAATTTCGA
CCTGGCAGAG
GATGCCAAGC
TGCAACTGTC
CAAGGACACC
TATGATGACG
ATCTCGACAA
CCTCCTGGCC
CAGATCGGTG
ACCAATACGC
CGACCTTTTC
CTTGCTGCTA
AGAATCTTTC
TGACGCCATC
CTGCTGTCTG
ACATTCTCCG
CGTGAACACT
GAAATCACCA
AGGCCCCTCT
TTCAGCTTCA
ATGATTAAGC
GGTATGATGA
GCACCACCAG
GACCTGACCC
TGCTTAAGGC
ACTCGTCCGG
CAGCAGCTTC
CGGAGAAGTA
CAAGGAAATC
TTCTTTGACC
AGTCAAAGAA
TGGATACGCC
GGCTACATCG
ACGGAGGTGC
CTCCCAAGAG
GAATTTTATA
AGTTTATCAA
ACCTATCCTT
GAGAAGATGG
ACGGCACCGA
AGAGCTCCTC
GTGAAACTGA
ATCGGGAGGA
TCTGCTGCGG
AAGCAGCGCA
CTTTCGACAA
TGGGAGCATT
CCCCACCAGA
TCCATCTTGG
GGAGCTTCAC
GCCATCCTTC
GGCGCCAAGA
GGACTTCTAC
CCCTTTCTTA
AGGACAACAG
GGAGAAGATT
GAGAAAATTC
TCACTTTCCG
CATCCCCTAC
TACGTGGGAC
CCCTCGCCAG
AGGAAATAGC
CGGTTTGCTT
GGATGACCAG
AAAGTCAGAA
GAAACTATCA
CTCCCTGGAA
CTTCGAAGAG
GTGGTGGACA
AGGGAGCCAG
CGCTCAGTCA
TTCATCGAAC
GGATGACTAA
CTTCGATAAG
AACCTCCCCA
ATGAGAAGGT
CCTGCCGAAA
CATTCCCTGC
TCTACGAGTA
CTTTACCGTG
TACAACGAGC
TGACCAAGGT
GAAATATGTC
ACCGAAGGGA
TGAGGAAGCC
CGCATTCCTG
TCAGGCGAAC
AAAAGAAGGC
AATTGTGGAC
CTTCTGTTCA
AGACCAATAG
AAAGGTGACC
GTGAAGCAGC
TGAAGGAGGA
CTATTTCAAG
AAAATTGAAT
GCTTCGACTC
TGTGGAGATT
AGCGGGGTCG
AAGATCGGTT
CAACGCAAGC
CTGGGTACTT
ACCATGATCT
GCTTAAGATC
ATCAAGGACA
AGGATTTTCT
GGACAATGAG
GAGAACGAGG
ACATCCTTGA
GGACATTGTC
CTGACTCTCA
CTCTGTTCGA
GGACCGGGAA
ATGATCGAGG
AGAGGCTTAA
GACCTACGCC
CATCTGTTCG
ACGATAAAGT
GATGAAGCAA
CTTAAACGGA
GAAGATATAC
CGGATGGGGA
CGCCTTAGCC
GCAAACTCAT
CAACGGAATC
CGGGACAAAC
AGAGCGGAAA
GACCATTCTT
GATTTCCTTA
AGAGCGACGG
ATTCGCTAAT
CGCAACTTCA
TGCAACTTAT
CCATGATGAT
TCCCTGACCT
TTAAGGAGGA
CATCCAGAAG
GCCCAAGTGT
CTGGACAAGG
TGACTCACTG
CACGAGCATA
TCGCAAATCT
GGCTGGTTCA
CCCGCTATTA
AGAAGGGTAT
TCTCCAGACC
GTGAAAGTCG
TGGACGAGCT
GGTCAAGGTG
ATGGGTCGCC
ATAAACCAGA
GAACATTGTC
ATCGAGATGG
CCAGGGAAAA
CCAGACTACC
CAGAAGGGAC
AGAAGAACAG
CAGGGAGCGG
ATGAAAAGAA
TTGAGGAAGG
GATTAAGGAG
CTCGGGTCAC
AGATCCTTAA
AGAGCACCCG
GTGGAAAACA
CCCAGCTTCA
GAATGAGAAG
CTCTATCTGT
ACTACCTTCA
AAATGGACGC
GATATGTATG
TGGACCAAGA
GCTTGATATC
AACAGGCTCT
CAGACTACGA
CGTGGACCAT
ATCGTCCCTC
AGAGCTTCCT
CAAAGACGAC
TCAATTGACA
ATAAGGTGCT
GACTCGCTCA
GACAAGAACC
GGGGAAAGTC
AGATAACGTG
CCCTCAGAGG
AAGTCGTGAA
AAAGATGAAG
AACTATTGGC
GCCAGCTTCT
GAACGCAAAG
CTGATCACTC
AGCGGAAGTT
CGACAATCTC
ACTAAGGCTG
AGAGGGGCGG
ACTGAGCGAA
CTGGACAAAG
CAGGATTCAT
TAAACGGCAA
CTTGTGGAGA
CTCGGCAGAT
TACTAAACAT
GTCGCCCAAA
TCCTTGACTC
ACGCATGAAT
ACCAAGTACG
ACGAAAACGA
CAAACTTATC
CGCGAGGTGA
AGGTGATTAC
CCTGAAGTCC
AAGCTGGTCA
GCGATTTCAG
AAAGGACTTT
CAATTCTACA
AAGTGCGGGA
GATCAATAAC
TATCATCATG
CTCATGACGC
ATATCTGAAT
GCCGTGGTGG
GAACCGCCCT
GATCAAGAAG
TACCCAAAGC
TGGAAAGCGA
GTTCGTGTAC
GGAGACTACA
AGGTCTACGA
CGTGCGCAAG
ATGATTGCCA
AATCTGAGCA
GGAGATCGGA
AAGGCCACCG
CAAAGTACTT
CTTCTACAGC
AACATCATGA
ATTTCTTCAA
GACCGAAATC
ACCCTTGCAA
ACGGTGAGAT
CCGGAAGAGG
CCGCTCATCG
AGACTAATGG
GGAGACTGGC
GAAATCGTGT
GGGACAAGGG
CAGAGATTTC
GCTACCGTGC
GCAAAGTGCT
TTCTATGCCT
CAAGTGAACA
TCGTGAAGAA
AACCGAGGTG
CAAACCGGAG
GCTTTTCTAA
GGAATCAATC
CTCCCCAAGC
GCAACTCCGA
CAAGCTCATT
GCAAGGAAGA
AGGATTGGGA
CCCTAAGAAG
TACGGCGGAT
TCGATTCACC
AACTGTGGCT
TATTCTGTCC
TGGTCGTGGC
TAAGGTGGAA
AAAGGAAAGT
CTAAGAAGCT
CAAGAGCGTG
AAGGAACTGC
TGGGTATCAC
CATTATGGAG
CGCAGCTCCT
TCGAGAAGAA
CCCAATTGAC
TTTCTCGAAG
CCAAAGGTTA
CAAGGAAGTC
AAGAAGGACC
TTATCATCAA
GCTCCCAAAG
TATAGCCTGT
TCGAACTGGA
GAATGGGCGG
AAGCGGATGC
TCGCCTCCGC
TGGCGAACTT
CAGAAGGGTA
ATGAGCTGGC
TCTCCCCTCC
AAGTACGTGA
ATTTCCTCTA
CCTTGCAAGC
CATTACGAGA
AGCTGAAGGG
GAGCCCCGAG
GACAACGAGC
AAAAGCAACT
GTTTGTGGAG
CAGCATAAGC
ATTATCTGGA
CGAGATCATT
GAGCAGATTT
CCGAGTTTTC
TAAACGCGTC
ATTCTCGCTG
ATGCCAACCT
CGATAAAGTC
CTTAGCGCAT
ACAATAAGCA
CAGAGACAAA
CCAATTCGGG
AGCAGGCTGA
GAATATCATC
CACCTGTTCA
CCCTCACCAA
TCTTGGTGCC
CCTGCCGCAT
TCAAGTACTT
CGACACCACC
ATCGACCGGA
AACGCTATAC
CTCCACCAAA
GAAGTGCTGG
ACGCCACCCT
CATCCACCAG
AGCATCACCG
GACTTTACGA
AACTCGGATT
GACCTCTCAC
AGCTCGGAGG
GGATGAGGGA
GCTGATCCAA
AAAAGAAGAG
AAAGGTAGAT
CCAAAAAAGA
AGAGAAAGGT
ATAAGGATCC
TAACTCGAGA
TTGAATTGAA
TTGAAATCGA
TAGATCAATT
TTTTTCTTTT
CTCTTTCCCC
ATCCTTTACG
CTAAAATAAT
AGTTTATTTT
ATTTTTTGAA
TATTTTTTAT
TTATATACGT
ATATATAGAC
TATTATTTAT
CTTTTAATGA
TTATTAAGAT
TTTTATTAAA
AAAAAATTCG
CTCCTCTTTT
AATGCCTTTA
TGCAGTTTTT
TTTTCCCATT
CGATATTTCT
ATGTGCTGCC
AAGATATCAA
CAACAGAGGA
CATATGCCCT
ACCTCCATGA
TCTACTGACG
TCCCTGAGGC
TGCAATTCAT
GTAATGGGGC
AGTATCCGCG
GCAAGTCCTA
GTGCAATGGC
GGTTTTTTAC
CCTCGTTCTG
AAGAAGAGGC
GACGCGGGTG
CGGTCATCAC
TAATGTGGAA
ATTGGTAAGA
CTCTCGGGCC
TCCGCCTTTA
GGCGGTGCTT
ACTCTTTCAT
AAAGGGGCTG
TTAGTTATGC
AACGATGTGC
TTCAGTATTA
CATTTTTTGC
CTTCAACGCC
TTGATTGTTC
TATTTTTGCT
AATAATAAAT
CTATTTCATC
GGACTAAAAG
TCCATTAGTT
GTAAGCGGAT
TTAGCTCAGT
TGGGAGAGCG
CCAGACTGAA
GAAAAACTTC
GGTCAAGTCA
TCTGGAGGTC
CTGTGTTCGA
TCCACAGAAT
TCGCAGATGG
CCGGCATGGT
CCCAGCCTCC
TCGCTGGCGC
CGGCTGGGCA
ACACCTTCGG
GTGGCGAATG
GGACTTGAGA
CGGAAAGTGA
AACGTGATTT
CATGCGTCAT
TTTGAACATT
TTGTAAATCT
TATTTAATAA
TGTGTGCGGC
AATTCACATT
TAATTTATGA
ATGTTTTCTT
AACATCGCGG
CAACTCAAGA
AACGGCAGGT
TCGGATCTTA
GCTACTAGAG
AAAGAGGAGA
AATACTAGAT
GCGTAAAGGC
GAAGAGCTGT
TCACTGGTGT
CGTCCCTATT
CTGGTGGAAC
TGGATGGTGA
TGTCAACGGT
CATAAGTTTT
CCGTGCGTGG
CGAGGGTGAA
GGTGACGCAA
CTAATGGTAA
ACTGACGCTG
AAGTTCATCT
GTACTACTGG
TAAACTGCCG
GTTCCTTGGC
CGACTCTGGT
AACGACGCTG
ACTTATGGTG
TTCAGTGCTT
TGCTCGTTAT
CCGGACCATA
TGAAGCAGCA
TGACTTCTTC
AAGTCCGCCA
TGCCGGAAGG
CTATGTGCAG
GAACGCACGA
TTTCCTTTAA
GGATGACGGC
ACGTACAAAA
CGCGTGCGGA
AGTGAAATTT
GAAGGCGATA
CCCTGGTAAA
CCGCATTGAG
CTGAAAGGCA
TTGACTTTAA
AGAGGACGGC
AATATCCTGG
GCCATAAGCT
GGAATACAAT
TTTAACAGCC
ACAATGTTTA
CATCACCGCC
GATAAACAAA
AAAATGGCAT
TAAAGCGAAT
TTTAAAATTC
GCCACAACGT
GGAGGATGGC
AGCGTGCAGC
TGGCTGATCA
CTACCAGCAA
AACACTCCAA
TCGGTGATGG
TCCTGTTCTG
CTGCCAGACA
ATCACTATCT
GAGCACGCAA
AGCGTTCTGT
CTAAAGATCC
GAACGAGAAA
CGCGATCATA
TGGTTCTGCT
GGAGTTCGTA
ACCGCAGCGG
GCATCACGCA
TGGTATGGAT
GAACTGTACA
AATGACCAGG
CATCAAATAA
AACGAAAGGC
TCAGTCGAAA
GACTGGGCCT
TTCGTTTTAT
CTGTTGTTTG
TCGGTGAACG
CTCTCTACTA
GAGTCACACT
GGCTCACCTT
CGGGTGGGCC
TTTCTGCGTT
TATACGTCTC
AGTTTTAGAG
CTAGAAATAG
CAAGTTAAAA
TAAGGCTAGT
CCGTTATCAA
CTTGAAAAAG
TGGCACCGAG
TCGGTGCTTT
TTTTATTTTT
TGTCACTATT
GTTATGTAAA
ATGCCACCTC
TGACAGTATG
GAACGCAAAC
TTCTGTCTAG
TGGATAGCTG
GACATTCCAG
GCAGTGCCTC
TGCCGCCGGA
CCCCTCTCGT
GATTGGGTAG
CTGGACATGC
CCTTGTAAGA
TATAACAAGA
GCCTGCCTGT
CTAATGATCT
CACGGCGAAA
GTCGGGGAGA
CAGCAGCGGC
TGCAGACATT
ATACCGCAAC
AACACTAAGG
TGAGATAACT
CCGTACATTG
ACTACGCGTT
CCTCTAGACC
TTACTTGACC
GGATACAGTG
TCTTTGACAC
GTTTATGGGT
TACAGCAATC
ACATCCAAGA
CTGGCTATGC
ACGAAGCAAC
TCTTGAGTGT
TAAAATGTTG
ACCCCTGTAT
TTGGGATGCG
GGTAGTAGAT
GAGTGCAGGA
TATCAGCAGT
TACAGAGATG
TTACGAACCA
CTAGTGCACT
GCAGTACAAA
CACAGTCCTT
TCCCGCAATT
TTCTTTTTCT
ATTACTCTTG
GCCTCCTCTA
GTACACTCTA
TATTTTTTTA
TGCCTCGGTA
ATGATTTTCA
TTTTTTTTTT
TCCACCTAGC
GGATGACTCT
TTTTTTTTCT
TAGCGATTGG
CATTATCACA
TAATGAATTA
TACATTATAT
AAAGTAATGT
GATTTCTTCG
AAGAATATAC
TAAAAAATGA
GCAGGCAAGA
TAAACGAAGG
CAAAGATGAC
AGAGCAGAAA
GCCCTAGTAA
AGCGTATTAC
AAATGAAACC
AAGATTCAGA
TTGCGATCTC
TTTAAAGGGT
GGTCCCCTAG
CGATAGAGCA
CTCGATCTTC
CCAGAAAAAG
AGGCAGAAGC
AGTAGCAGAA
CAGGCCACAC
AATCGCAAGT
GATTAACGTC
CACACAGGTA
TAGGGTTTCT
GGACCATATG
ATACATGCTC
TGGCCAAGCA
TTCCGGCTGG
TCGCTAATCG
TTGAGTGCAT
TGGTGACTTA
CACATAGACG
ACCATCACAC
CACTGAGGAC
TGCGGGATTG
CTCTCGGTCA
AGCTTTTAAA
GAGGCCCTAG
GGGCCGTGCG
TGGAGTAAAA
AGGTTTGGAT
CAGGATTTGC
GCCTTTGGAT
GAGGCACTTT
CCAGAGCGGT
GGTTGATCTT
TCGAACAGGC
CGTACGCAGT
TGTCGAACTT
GGTTTGCAAA
GGGAGAAAGT
AGGTGATCTC
TCTTGCGAGA
TGATCCCGCA
TTTTCTTGAA
AGCTTTGCAG
AGGCTAGCAG
AATTACCCTC
CACGTTGATT
GTCTGCGAGG
CAAGAATGAT
CATCACCGTA
GTGAGAGTGC
GTTCAAGGCT
CTTGCGGTTG
CCATAAGAGA
AGCCACCTCG
CCCAATGGTA
CCAACGATGT
TCCCTCCACC
AAAGGTGTTC
TTATGTAGTG
ACACCGATTA
TTTAAAGCTG
CTGCATACGA
TATATATACA
TGTGTATATA
TGTATACCTA
TGAATGTCAG
TAAGTATGTA
TACGAACAGT
ATGATACTGA
AGATGACAAG
GTAATGCATC
ATTCTATACG
TGTCATTCTG
AACGAGGCGC
GCTTTCCTTT
TTTCTTTTTG
CTTTTTCTTT
TTTTTTCTCT
TGAACTCGAC
GGATCATAGA
GTAACGAAGC
ATCTGTGCTT
CATTTTGTAG
AACAAAAATG
CAACGCGAGA
GCGCTAATTT
TTCAAACAAA
GAATCTGAGC
TGCATTTTTA
CAGAACAGAA
ATGCAACGCG
AAAGCGCTAT
TTTACCAACG
AAGAATCTGT
GCTTCATTTT
TGTAAAACAA
AAATGCAACG
CGAGAGCGCT
AATTTTTCAA
ACAAAGAATC
TGAGCTGCAT
TTTTACAGAA
CAGAAATGCA
ACGCGAGAGC
GCTATTTTAC
CAACAAAGAA
TCTATACTTC
TTTTTTGTTC
TACAAAAATG
CATCCCGAGA
GCGCTATTTT
TCTAACAAAG
CATCTTAGAT
TACTTTTTTT
CTCCTTTGTG
CGCTCTATAA
TGCAGTCTCT
TGATAACTTT
TTGCACTGTA
GGTCCGTTAA
GGTTAGAAGA
AGGCTACTTT
GGTGTCTATT
TTCTCTTCCA
TAAAAAAAGC
CTGACTCCAC
TTCCCGCGTT
TACTGATTAC
TAGCGAAGCT
GCGGGTGCAT
TTTTTCAAGA
TAAAGGCATC
CCCGATTATA
TTCTATACCG
ATGTGGATTG
CGCATACTTT
GTGAACAGAA
AGTGATAGCG
TTGATGATTC
TTCATTGGTC
AGAAAATTAT
GAACGGTTTC
TTCTATTTTG
TCTCTATATA
CTACGTATAG
GAAATGTTTA
CATTTTCGTA
TTGTTTTCGA
TTCACTCTAT
GAATAGTTCT
TACTACAATT
TTTTTGTCTA
AAGAGTAATA
CTAGAGATAA
ACATAAAAAA
TGTAGAGGTC
GAGTTTAGAT
GCAAGTTCAA
GGAGCGAAAG
GTGGATGGGT
AGGTTATATA
GGGATATAGC
ACAGAGATAT
ATAGCAAAGA
GATACTTTTG
AGCAATGTTT
GTGGAAGCGG
TATTCGCAAT
ATTTTAGTAG
CTCGTTACAG
TCCGGTGCGT
TTTTGGTTTT
TTGAAAGTGC
GTCATCAGAG
CGCTTTTGGT
TTTCAAAAGC
GCTCTGAAGT
TCCTATACTT
TCTAGCTAGA
GAATAGGAAC
TTCCCGAGCG
GCCGCGTGTT
ACAACCAATT
AACCAATTCT
GATTAGAAAA
ACTCATCGAG
CATCAAATGA
AACTGCAATT
TATTCATATC
AGGATTATCA
ATACCATATT
TTTGAAAAAG
CCGTTTCTGT
AATGAAGGAG
AAAACTCACC
GAGGCAGTTC
CATAGGATGG
CAAGATCCTG
GTATCGGTCT
GCGATTCCGA
CTCGTCCAAC
ATCAATACAA
CCTATTAATT
TCCCCTCGTC
AAAAATAAGG
TTATCAAGTG
AGAAATCACC
ATGAGTGACG
ACTGAATCCG
GTGAGAATGG
CAAAAGCTTA
TGCATTTCTT
TCCAGACTTG
TTCAACAGGC
CAGCCATTAC
GCTCGTCATC
AAAATCACTC
GCATCAACCA
AACCGTTATT
CATTCGTGAT
TGCGCCTGAG
CGAGGCGAAA
TACGCGATCG
CTGTTAAAAG
GACAATTACA
AACAGGAATC
GAATGCAACC
GGCGCAGGAA
CACTGCCAGC
GCATCAACAA
TATTTTCACC
TGAATCAGGA
TATTCTTCTA
ATACCTGGAA
TGCTGTTTTC
CCGGGGATCG
CAGTGGTGAG
TAACCATGCA
TCATCAGGAG
TACGGATAAA
ATGCTTGATG
GTCGGAAGAG
GCATAAATTC
CGTCAGCCAG
TTTAGTCTGA
CCATCTCATC
TGTAACATCA
TTGGCAACGC
TACCTTTGCC
ATGTTTCAGA
AACAACTCTG
GCGCATCGGG
CTTCCCATAC
AATCGATAGA
TTGTCGCACC
TGATTGCCCG
ACATTATCGC
GAGCCCATTT
ATACCCATAT
AAATCAGCAT
CCATGTTGGA
ATTTAATCGC
GGCCTGGAGC
AAGACGTTTC
CCGTTGAATA
TGGCTCATAA
CACCCCTTGT
ATTACTGTTT
ATGTAAGCAG
ACAGTTTTAT
TGTTCATGAT
GATATATTTT
TATCTTGTGC
AATGTAACAT
CAGAGATTTT
GAGACACAAC
GTGGCTTTGT
TGAATAAATC
GAACTTTTGC
TGAGTTGAAG
GATCAGTCAT
GACCAAAATC
CCTTAACGTG
AGTTTTCGTT
CCACTGAGCG
TCAGACCCCG
TAGAAAAGAT
CAAAGGATCT
TC