名称: | pCMV-CUL4B(human)-EGFP-Neo |
---|---|
别称: | NM_003588.4;CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2 |
基因长: | 2739bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | 绿色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GACGGATCGG
GAGATCTCCC
GATCCCCTAT
GGTGCACTCT
CAGTACAATC
TGCTCTGATG
CCGCATAGTT
AAGCCAGTAT
CTGCTCCCTG
CTTGTGTGTT
GGAGGTCGCT
GAGTAGTGCG
CGAGCAAAAT
TTAAGCTACA
ACAAGGCAAG
GCTTGACCGA
CAATTGCATG
AAGAATCTGC
TTAGGGTTAG
GCGTTTTGCG
CTGCTTCGCG
ATGTACGGGC
CAGATATACG
CGTTGACATT
GATTATTGAC
TAGTTATTAA
TAGTAATCAA
TTACGGGGTC
ATTAGTTCAT
AGCCCATATA
TGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCT
CTGGCTAACT
AGAGAACCCA
CTGCTTACTG
GCTTATCGAA
ATTAATACGA
CTCACTATAG
GGAGACCCAA
GCTGGCTAGC
GTTTAAAGCC
ACTGGGATCC
GCCACCATGA
TGTCACAGTC
ATCTGGATCA
GGAGATGGGA
ATGATGATGA
GGCTACTACC
TCTAAAGACG
GTGGTTTTTC
TTCCCCCAGT
CCCTCAGCTG
CTGCTGCTGC
TCAGGAGGTC
AGATCTGCCA
CTGATGGTAA
TACCAGCACC
ACTCCGCCCA
CCTCTGCCAA
GAAGAGAAAG
TTAAACAGCA
GCAGCAGTAG
CAGCAGTAAC
AGTAGTAACG
AGAGAGAAGA
CTTTGATTCC
ACCTCTTCCT
CCTCTTCCAC
TCCTCCTTTA
CAACCCAGGG
ATTCGGCATC
CCCTTCAACC
TCGTCCTTCT
GCCTGGGGGT
TTCAGTGGCT
GCTTCCAGCC
ACGTACCGAT
ACAGAAGAAG
CTGCGTTTTG
AAGACACCCT
GGAGTTTGTA
GGGTTTGATG
CGAAGATGGC
TGAGGAATCC
TCCTCCTCCT
CCTCCTCATC
TTCACCAACT
GCTGCAACAT
CTCAGCAGCA
GCAACTTAAA
AATAAGAGTA
TATTAATCTC
TTCTGTGGCT
TCGGTGCATC
ATGCAAACGG
CCTAGCCAAA
TCTTCTACCA
CCGTCTCTAG
CTTTGCTAAC
AGCAAACCTG
GCTCTGCTAA
GAAGTTAGTG
ATCAAGAACT
TTAAAGATAA
GCCTAAATTA
CCAGAAAACT
ACACAGATGA
AACCTGGCAA
AAACTGAAAG
AAGCAGTGGA
AGCTATTCAG
AATAGTACTT
CAATTAAGTA
CAATTTAGAA
GAACTCTACC
AGGCTGTAGA
AAATCTCTGT
TCTTACAAGA
TTTCTGCAAA
CTTGTACAAA
CAGCTGAGAC
AGATCTGCGA
AGATCACATC
AAAGCACAGA
TTCATCAATT
CAGAGAGGAT
TCATTGGATA
GCGTTCTTTT
TTTAAAGAAG
ATTGATAGAT
GCTGGCAAAA
CCATTGCAGA
CAAATGATCA
TGATCAGGAG
CATTTTTTTG
TTTCTGGATA
GAACTTACGT
TCTTCAGAAT
TCAATGCTAC
CCTCCATTTG
GGACATGGGA
CTGGAGTTAT
TTAGGGCTCA
TATTATAAGT
GATCAGAAAG
TGCAGAATAA
GACAATTGAT
GGCATTCTTC
TCTTGATTGA
GAGGGAAAGG
AATGGTGAAG
CAATTGATAG
AAGTTTACTT
CGAAGCCTTT
TAAGCATGCT
GTCTGATTTG
CAAATTTATC
AAGATTCTTT
TGAACAACGA
TTTTTGGAAG
AAACTAACCG
GCTCTATGCA
GCTGAAGGCC
AAAAATTAAT
GCAAGAAAGA
GAGGTTCCTG
AATATCTACA
TCATGTTAAC
AAACGTCTAG
AAGAAGAAGC
AGACAGACTT
ATTACTTACT
TAGATCAGAC
CACCCAGAAG
TCATTAATTG
CTACTGTAGA
AAAACAACTT
CTAGGTGAAC
ACTTAACAGC
AATTCTTCAG
AAAGGTTTAA
ATAACCTCCT
TGATGAAAAC
CGAATTCAAG
ATTTGTCTCT
TCTGTATCAG
CTCTTCAGTA
GAGTTCGAGG
TGGAGTTCAG
GTTCTTTTGC
AGCAGTGGAT
CGAATATATC
AAGGCATTTG
GCAGCACTAT
TGTAATTAAT
CCTGAAAAAG
ATAAAACCAT
GGTTCAAGAA
TTGCTGGATT
TTAAAGATAA
GGTTGACCAT
ATAATTGATA
TCTGCTTTCT
GAAGAATGAG
AAATTTATCA
ATGCCATGAA
AGAAGCATTT
GAAACGTTCA
TTAACAAAAG
ACCAAATAAA
CCAGCTGAAC
TTATAGCTAA
GTATGTAGAT
TCAAAACTTC
GTGCAGGCAA
CAAAGAAGCT
ACAGATGAAG
AACTTGAGAA
AATGTTGGAT
AAAATTATGA
TCATATTTAG
ATTTATCTAT
GGCAAGGATG
TTTTTGAGGC
CTTCTATAAG
AAAGATTTAG
CCAAGCGCCT
GTTAGTCGGA
AAGAGTGCAT
CTGTAGATGC
TGAAAAATCA
ATGCTGTCCA
AACTTAAACA
TGAATGCGGA
GCTGCTTTCA
CCAGCAAACT
TGAAGGAATG
TTTAAAGACA
TGGAACTTTC
TAAAGACATC
ATGATTCAGT
TCAAACAGTA
TATGCAGAAT
CAGAATGTTC
CGGGAAATAT
TGAGTTAACT
GTGAATATCC
TGACAATGGG
CTATTGGCCG
ACATATGTGC
CTATGGAAGT
TCATTTACCA
CCAGAGATGG
TAAAACTTCA
GGAGATTTTC
AAGACATTTT
ACCTAGGCAA
ACATAGTGGC
AGGAAACTTC
AGTGGCAGTC
AACCCTAGGA
CACTGTGTGT
TAAAAGCAGA
ATTTAAAGAG
GGTAAAAAGG
AACTCCAGGT
CTCTCTTTTT
CAAACACTGG
TGCTGCTAAT
GTTTAATGAG
GGAGAGGAGT
TCAGTTTAGA
AGAGATCAAG
CAGGCAACTG
GAATAGAGGA
TGGAGAGTTA
AGGAGAACAC
TGCAGTCATT
AGCCTGTGGC
AAAGCTAGAG
TTCTGGCGAA
AAATCCAAAG
GGCAAAGACA
TTGAAGATGG
TGACAAGTTC
ATTTGTAATG
ATGATTTCAA
ACATAAACTT
TTCAGGATAA
AGATCAATCA
AATCCAGATG
AAAGAAACGG
TTGAAGAACA
AGCAAGCACT
ACAGAAAGAG
TATTTCAAGA
CAGACAGTAT
CAAATTGATG
CTGCAATTGT
TCGAATTATG
AAGATGAGAA
AGACACTTAG
CCACAATCTC
CTTGTTTCAG
AAGTGTACAA
CCAGTTGAAA
TTTCCAGTAA
AGCCTGCTGA
TCTTAAGAAG
AGAATAGAAT
CTTTAATTGA
CCGGGACTAC
ATGGAAAGAG
ATAAAGAAAA
TCCAAACCAG
TACAACTATA
TTGCAGGCTC
GAGTGGCGGT
GGAGGAAGCG
GCGGAGGTGG
ATCTATGGTG
AGCAAGGGCG
AGGAGCTGTT
CACCGGGGTG
GTGCCCATCC
TGGTCGAGCT
GGACGGCGAC
GTAAACGGCC
ACAAGTTCAG
CGTGTCCGGC
GAGGGCGAGG
GCGATGCCAC
CTACGGCAAG
CTGACCCTGA
AGTTCATCTG
CACCACCGGC
AAGCTGCCCG
TGCCCTGGCC
CACCCTCGTG
ACCACCCTGA
CCTACGGCGT
GCAGTGCTTC
AGCCGCTACC
CCGACCACAT
GAAGCAGCAC
GACTTCTTCA
AGTCCGCCAT
GCCCGAAGGC
TACGTCCAGG
AGCGCACCAT
CTTCTTCAAG
GACGACGGCA
ACTACAAGAC
CCGCGCCGAG
GTGAAGTTCG
AGGGCGACAC
CCTGGTGAAC
CGCATCGAGC
TGAAGGGCAT
CGACTTCAAG
GAGGACGGCA
ACATCCTGGG
GCACAAGCTG
GAGTACAACT
ACAACAGCCA
CAACGTCTAT
ATCATGGCCG
ACAAGCAGAA
GAACGGCATC
AAGGTGAACT
TCAAGATCCG
CCACAACATC
GAGGACGGCA
GCGTGCAGCT
CGCCGACCAC
TACCAGCAGA
ACACCCCCAT
CGGCGACGGC
CCCGTGCTGC
TGCCCGACAA
CCACTACCTG
AGCACCCAGT
CCGCCCTGAG
CAAAGACCCC
AACGAGAAGC
GCGATCACAT
GGTCCTGCTG
GAGTTCGTGA
CCGCCGCCGG
GATCACTCTC
GGCATGGACG
AGCTGTACAA
GTAGTCTAGA
GGGCCCGTTT
AAACCCGCTG
ATCAGCCTCG
ACTGTGCCTT
CTAGTTGCCA
GCCATCTGTT
GTTTGCCCCT
CCCCCGTGCC
TTCCTTGACC
CTGGAAGGTG
CCACTCCCAC
TGTCCTTTCC
TAATAAAATG
AGGAAATTGC
ATCGCATTGT
CTGAGTAGGT
GTCATTCTAT
TCTGGGGGGT
GGGGTGGGGC
AGGACAGCAA
GGGGGAGGAT
TGGGAAGACA
ATAGCAGGCA
TGCTGGGGAT
GCGGTGGGCT
CTATGGCTTC
TGAGGCGGAA
AGAACCAGCT
GGGGCTCTAG
GGGGTATCCC
CACGCGCCCT
GTAGCGGCGC
ATTAAGCGCG
GCGGGTGTGG
TGGTTACGCG
CAGCGTGACC
GCTACACTTG
CCAGCGCCCT
AGCGCCCGCT
CCTTTCGCTT
TCTTCCCTTC
CTTTCTCGCC
ACGTTCGCCG
GCTTTCCCCG
TCAAGCTCTA
AATCGGGGGC
TCCCTTTAGG
GTTCCGATTT
AGTGCTTTAC
GGCACCTCGA
CCCCAAAAAA
CTTGATTAGG
GTGATGGTTC
ACGTAGTGGG
CCATCGCCCT
GATAGACGGT
TTTTCGCCCT
TTGACGTTGG
AGTCCACGTT
CTTTAATAGT
GGACTCTTGT
TCCAAACTGG
AACAACACTC
AACCCTATCT
CGGTCTATTC
TTTTGATTTA
TAAGGGATTT
TGCCGATTTC
GGCCTATTGG
TTAAAAAATG
AGCTGATTTA
ACAAAAATTT
AACGCGAATT
AATTCTGTGG
AATGTGTGTC
AGTTAGGGTG
TGGAAAGTCC
CCAGGCTCCC
CAGCAGGCAG
AAGTATGCAA
AGCATGCATC
TCAATTAGTC
AGCAACCAGG
TGTGGAAAGT
CCCCAGGCTC
CCCAGCAGGC
AGAAGTATGC
AAAGCATGCA
TCTCAATTAG
TCAGCAACCA
TAGTCCCGCC
CCTAACTCCG
CCCATCCCGC
CCCTAACTCC
GCCCAGTTCC
GCCCATTCTC
CGCCCCATGG
CTGACTAATT
TTTTTTATTT
ATGCAGAGGC
CGAGGCCGCC
TCTGCCTCTG
AGCTATTCCA
GAAGTAGTGA
GGAGGCTTTT
TTGGAGGCCT
AGGCTTTTGC
AAAAAGCTCC
CGGGAGCTTG
TATATCCATT
TTCGGATCTG
ATCAAGAGAC
AGGATGAGGA
TCGTTTCGCA
TGATTGAACA
AGATGGATTG
CACGCAGGTT
CTCCGGCCGC
TTGGGTGGAG
AGGCTATTCG
GCTATGACTG
GGCACAACAG
ACAATCGGCT
GCTCTGATGC
CGCCGTGTTC
CGGCTGTCAG
CGCAGGGGCG
CCCGGTTCTT
TTTGTCAAGA
CCGACCTGTC
CGGTGCCCTG
AATGAACTGC
AGGACGAGGC
AGCGCGGCTA
TCGTGGCTGG
CCACGACGGG
CGTTCCTTGC
GCAGCTGTGC
TCGACGTTGT
CACTGAAGCG
GGAAGGGACT
GGCTGCTATT
GGGCGAAGTG
CCGGGGCAGG
ATCTCCTGTC
ATCTCACCTT
GCTCCTGCCG
AGAAAGTATC
CATCATGGCT
GATGCAATGC
GGCGGCTGCA
TACGCTTGAT
CCGGCTACCT
GCCCATTCGA
CCACCAAGCG
AAACATCGCA
TCGAGCGAGC
ACGTACTCGG
ATGGAAGCCG
GTCTTGTCGA
TCAGGATGAT
CTGGACGAAG
AGCATCAGGG
GCTCGCGCCA
GCCGAACTGT
TCGCCAGGCT
CAAGGCGCGC
ATGCCCGACG
GCGAGGATCT
CGTCGTGACC
CATGGCGATG
CCTGCTTGCC
GAATATCATG
GTGGAAAATG
GCCGCTTTTC
TGGATTCATC
GACTGTGGCC
GGCTGGGTGT
GGCGGACCGC
TATCAGGACA
TAGCGTTGGC
TACCCGTGAT
ATTGCTGAAG
AGCTTGGCGG
CGAATGGGCT
GACCGCTTCC
TCGTGCTTTA
CGGTATCGCC
GCTCCCGATT
CGCAGCGCAT
CGCCTTCTAT
CGCCTTCTTG
ACGAGTTCTT
CTGAGCGGGA
CTCTGGGGTT
CGAAATGACC
GACCAAGCGA
CGCCCAACCT
GCCATCACGA
GATTTCGATT
CCACCGCCGC
CTTCTATGAA
AGGTTGGGCT
TCGGAATCGT
TTTCCGGGAC
GCCGGCTGGA
TGATCCTCCA
GCGCGGGGAT
CTCATGCTGG
AGTTCTTCGC
CCACCCCAAC
TTGTTTATTG
CAGCTTATAA
TGGTTACAAA
TAAAGCAATA
GCATCACAAA
TTTCACAAAT
AAAGCATTTT
TTTCACTGCA
TTCTAGTTGT
GGTTTGTCCA
AACTCATCAA
TGTATCTTAT
CATGTCTGTA
TACCGTCGAC
CTCTAGCTAG
AGCTTGGCGT
AATCATGGTC
ATAGCTGTTT
CCTGTGTGAA
ATTGTTATCC
GCTCACAATT
CCACACAACA
TACGAGCCGG
AAGCATAAAG
TGTAAAGCCT
GGGGTGCCTA
ATGAGTGAGC
TAACTCACAT
TAATTGCGTT
GCGCTCACTG
CCCGCTTTCC
AGTCGGGAAA
CCTGTCGTGC
CAGCTGCATT
AATGAATCGG
CCAACGCGCG
GGGAGAGGCG
GTTTGCGTAT
TGGGCGCTCT
TCCGCTTCCT
CGCTCACTGA
CTCGCTGCGC
TCGGTCGTTC
GGCTGCGGCG
AGCGGTATCA
GCTCACTCAA
AGGCGGTAAT
ACGGTTATCC
ACAGAATCAG
GGGATAACGC
AGGAAAGAAC
ATGTGAGCAA
AAGGCCAGCA
AAAGGCCAGG
AACCGTAAAA
AGGCCGCGTT
GCTGGCGTTT
TTCCATAGGC
TCCGCCCCCC
TGACGAGCAT
CACAAAAATC
GACGCTCAAG
TCAGAGGTGG
CGAAACCCGA
CAGGACTATA
AAGATACCAG
GCGTTTCCCC
CTGGAAGCTC
CCTCGTGCGC
TCTCCTGTTC
CGACCCTGCC
GCTTACCGGA
TACCTGTCCG
CCTTTCTCCC
TTCGGGAAGC
GTGGCGCTTT
CTCATAGCTC
ACGCTGTAGG
TATCTCAGTT
CGGTGTAGGT
CGTTCGCTCC
AAGCTGGGCT
GTGTGCACGA
ACCCCCCGTT
CAGCCCGACC
GCTGCGCCTT
ATCCGGTAAC
TATCGTCTTG
AGTCCAACCC
GGTAAGACAC
GACTTATCGC
CACTGGCAGC
AGCCACTGGT
AACAGGATTA
GCAGAGCGAG
GTATGTAGGC
GGTGCTACAG
AGTTCTTGAA
GTGGTGGCCT
AACTACGGCT
ACACTAGAAG
AACAGTATTT
GGTATCTGCG
CTCTGCTGAA
GCCAGTTACC
TTCGGAAAAA
GAGTTGGTAG
CTCTTGATCC
GGCAAACAAA
CCACCGCTGG
TAGCGGTTTT
TTTGTTTGCA
AGCAGCAGAT
TACGCGCAGA
AAAAAAGGAT
CTCAAGAAGA
TCCTTTGATC
TTTTCTACGG
GGTCTGACGC
TCAGTGGAAC
GAAAACTCAC
GTTAAGGGAT
TTTGGTCATG
AGATTATCAA
AAAGGATCTT
CACCTAGATC
CTTTTAAATT
AAAAATGAAG
TTTTAAATCA
ATCTAAAGTA
TATATGAGTA
AACTTGGTCT
GACAGTTACC
AATGCTTAAT
CAGTGAGGCA
CCTATCTCAG
CGATCTGTCT
ATTTCGTTCA
TCCATAGTTG
CCTGACTCCC
CGTCGTGTAG
ATAACTACGA
TACGGGAGGG
CTTACCATCT
GGCCCCAGTG
CTGCAATGAT
ACCGCGAGAC
CCACGCTCAC
CGGCTCCAGA
TTTATCAGCA
ATAAACCAGC
CAGCCGGAAG
GGCCGAGCGC
AGAAGTGGTC
CTGCAACTTT
ATCCGCCTCC
ATCCAGTCTA
TTAATTGTTG
CCGGGAAGCT
AGAGTAAGTA
GTTCGCCAGT
TAATAGTTTG
CGCAACGTTG
TTGCCATTGC
TACAGGCATC
GTGGTGTCAC
GCTCGTCGTT
TGGTATGGCT
TCATTCAGCT
CCGGTTCCCA
ACGATCAAGG
CGAGTTACAT
GATCCCCCAT
GTTGTGCAAA
AAAGCGGTTA
GCTCCTTCGG
TCCTCCGATC
GTTGTCAGAA
GTAAGTTGGC
CGCAGTGTTA
TCACTCATGG
TTATGGCAGC
ACTGCATAAT
TCTCTTACTG
TCATGCCATC
CGTAAGATGC
TTTTCTGTGA
CTGGTGAGTA
CTCAACCAAG
TCATTCTGAG
AATAGTGTAT
GCGGCGACCG
AGTTGCTCTT
GCCCGGCGTC
AATACGGGAT
AATACCGCGC
CACATAGCAG
AACTTTAAAA
GTGCTCATCA
TTGGAAAACG
TTCTTCGGGG
CGAAAACTCT
CAAGGATCTT
ACCGCTGTTG
AGATCCAGTT
CGATGTAACC
CACTCGTGCA
CCCAACTGAT
CTTCAGCATC
TTTTACTTTC
ACCAGCGTTT
CTGGGTGAGC
AAAAACAGGA
AGGCAAAATG
CCGCAAAAAA
GGGAATAAGG
GCGACACGGA
AATGTTGAAT
ACTCATACTC
TTCCTTTTTC
AATATTATTG
AAGCATTTAT
CAGGGTTATT
GTCTCATGAG
CGGATACATA
TTTGAATGTA
TTTAGAAAAA
TAAACAAATA
GGGGTTCCGC
GCACATTTCC
CCGAAAAGTG
CCACCTGACG
TC