名称: | AAVS1-idCas9-vpr |
---|---|
别称: | Plasmid#89985 |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达,四环素诱导 |
片段类型: | ORF |
片段物种: | |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | TRE-tight |
复制子: | pUC |
拷贝数: | |
感受态: | DB3.1 |
温度: | 37度 |
背景载体: | |
正向引物: | T7 |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACTCCGGGGA
GCACGACGGC
GCTGCCCAGA
CCCTTGCCCT
GGTGGTCGGG
CGAGACGCCG
ACGGTGGCCA
GGAACCACGC
GGGCTCCTTG
GGCCGGTGCG
GCGCCAGGAG
GCCTTCCATC
TGTTGCTGCG
CGGCCAGCCG
GGAACCGCTC
AACTCGGCCA
TGCGCGGGCC
GATCTCGGCG
AACACCGCCC
CCGCTTCGAC
GCTCTCCGGC
GTGGTCCAGA
CCGCCACCGC
GGCGCCGTCG
TCCGCGACCC
ACACCTTGCC
GATGTCGAGC
CCGACGCGCG
TGAGGAAGAG
TTCTTGCAGC
TCGGTGACCC
GCTCGATGTG
GCGGTCCGGA
TCGACGGTGT
GGCGCGTGGC
GGGGTAGTCG
GCGAACGCGG
CGGCGAGGGT
GCGTACGGCC
CTGGGGACGT
CGTCGCGGGT
GGCGAGGCGC
ACCGTGGGCT
TGTACTCGGT
CATCTCGAGC
CTAGGGCCGG
GATTCTCCTC
CACGTCACCG
CATGTTAGAA
GACTTCCTCT
GCCCTCTCCG
CTGCCAGATC
TCTCGAGGCC
CTGTGGGAGG
AAGAGAAGAG
GTCAGAAGCT
TGCCCCACTG
TGGGGTGGAG
GGGACAGATA
AAAGTACCCA
GAACCAGAGC
CACATTAACC
GGCCCTGGGA
ATATAAGGTG
GTCCCAGCTC
GGGGACACAG
GATCCCTGGA
GGCAGCAAAC
ATGCTGTCCT
GAAGTGGACA
TAGGGGCCCG
GGTTGGAGGA
AGAAGACTAG
CTGAGCTCTC
GGACCCCTGG
AAGATGCCAT
GACAGGGGGC
TGGAAGAGCT
AGCACAGACT
AGAGAGGTAA
GGGGGGTAGG
GGAGCTGCCC
AAATGAAAGG
AGTGAGAGGT
GACCCGAATC
CACAGGAGAA
CGGGGTGTCC
AGGCAAAGAA
AGCAAGAGGA
TGGAGAGGTG
GCTAAAGCCA
GGGAGACGGG
GTACTTTGGG
GTTGTCCAGA
AAAACGGTGA
TGATGCAGGC
CTACAAGAAG
GGGAGGCGGG
ACGCAAGGGA
GACATCCGTC
GGAGAAGGCC
ATCCTAAGAA
ACGAGAGATG
GCACAGGCCC
CAGAAGGAGA
AGGAAAAGGG
AACCCAGCGA
GTGAAGACGG
CATGGGGTTG
GGTGAGGGAG
GAGAGATGCC
CGGAGAGGAC
CCAGACACGG
GGAGGATCCG
CTCAGAGGAC
ATCACGTGGT
GCAGCGCCGA
GAAGGAAGTG
CTCCGGAAAG
AGCATCCTTG
GGCAGCAACA
CAGCAGAGAG
CAAGGGGAAG
AGGGAGTGGA
GGAAGACGGA
ACCTGAAGGA
GGCGGCAGGG
AAGGATCTGG
GCCAGCCGTA
GAGGTGACCC
AGGCCACAAG
CTGCAGACAG
AAAGCGGCAC
AGGCCCAGGG
GAGAGAATGC
TGGTCAGAGA
AAGCAAAGGG
CGAATTCGTT
TAAACCTGCA
GGACTAGTCC
CTTTAGTGAG
GGTTAATTCT
GAGCTTGGCG
TAATCATGGT
CATAGCTGTT
TCCTGTGTGA
AATTGTTATC
CGCTCACAAT
TCCACACAAC
ATACGAGCCG
GAAGCATAAA
GTGTAAAGCC
TGGGGTGCCT
AATGAGTGAG
CTAACTCACA
TTAATTGCGT
TGCGCTCACT
GCCCGCTTTC
CAGTCGGGAA
ACCTGTCGTG
CCAGCTGCAT
TAATGAATCG
GCCAACGCGC
GGGGAGAGGC
GGTTTGCGTA
TTGGGCGCTC
TTCCGCTTCC
TCGCTCACTG
ACTCGCTGCG
CTCGGTCGTT
CGGCTGCGGC
GAGCGGTATC
AGCTCACTCA
AAGGCGGTAA
TACGGTTATC
CACAGAATCA
GGGGATAACG
CAGGAAAGAA
CATGTGAGCA
AAAGGCCAGC
AAAAGGCCAG
GAACCGTAAA
AAGGCCGCGT
TGCTGGCGTT
TTTCCATAGG
CTCCGCCCCC
CTGACGAGCA
TCACAAAAAT
CGACGCTCAA
GTCAGAGGTG
GCGAAACCCG
ACAGGACTAT
AAAGATACCA
GGCGTTTCCC
CCTGGAAGCT
CCCTCGTGCG
CTCTCCTGTT
CCGACCCTGC
CGCTTACCGG
ATACCTGTCC
GCCTTTCTCC
CTTCGGGAAG
CGTGGCGCTT
TCTCATAGCT
CACGCTGTAG
GTATCTCAGT
TCGGTGTAGG
TCGTTCGCTC
CAAGCTGGGC
TGTGTGCACG
AACCCCCCGT
TCAGCCCGAC
CGCTGCGCCT
TATCCGGTAA
CTATCGTCTT
GAGTCCAACC
CGGTAAGACA
CGACTTATCG
CCACTGGCAG
CAGCCACTGG
TAACAGGATT
AGCAGAGCGA
GGTATGTAGG
CGGTGCTACA
GAGTTCTTGA
AGTGGTGGCC
TAACTACGGC
TACACTAGAA
GAACAGTATT
TGGTATCTGC
GCTCTGCTGA
AGCCAGTTAC
CTTCGGAAAA
AGAGTTGGTA
GCTCTTGATC
CGGCAAACAA
ACCACCGCTG
GTAGCGGTGG
TTTTTTTGTT
TGCAAGCAGC
AGATTACGCG
CAGAAAAAAA
GGATCTCAAG
AAGATCCTTT
GATCTTTTCT
ACGGGGTCTG
ACGCTCAGTG
GAACGAAAAC
TCACGTTAAG
GGATTTTGGT
CATGAGACAA
TAACCCTGAT
AAATGCTTCA
ATAATATTGA
AAAAGGAAGA
GTATGAGTAT
TCAACATTTC
CGTGTCGCCC
TTATTCCCTT
TTTTGCGGCA
TTTTGCCTTC
CTGTTTTTGC
TCACCCAGAA
ACGCTGGTGA
AAGTAAAAGA
TGCTGAAGAT
CAGTTGGGTG
CACGAGTGGG
TTACATCGAA
CTGGATCTCA
ACAGCGGTAA
GATCCTTGAG
AGTTTTCGCC
CCGAAGAACG
TTTTCCAATG
ATGAGCACTT
TTAAAGTTCT
GCTATGTGGC
GCGGTATTAT
CCCGTATTGA
CGCCGGGCAA
GAGCAACTCG
GTCGCCGCAT
ACACTATTCT
CAGAATGACT
TGGTTGAGTA
TTCACCAGTC
ACAGAAAAGC
ATCTTACGGA
TGGCATGACA
GTAAGAGAAT
TATGCAGTGC
TGCCATAACC
ATGAGTGATA
ACACTGCGGC
CAACTTACTT
CTGACAACGA
TCGGAGGACC
GAAGGAGCTA
ACCGCTTTTT
TGCACAACAT
GGGGGATCAT
GTAACTCGCC
TTGATCGTTG
GGAACCGGAG
CTGAATGAAG
CCATACCAAA
CGACGAGCGT
GACACCACGA
TGCCTGTAGC
AATGGCAACA
ACGTTGCGCA
AACTATTAAC
TGGCGAACTA
CTTACTCTAG
CTTCCCGGCA
ACAATTAATA
GACTGGATGG
AGGCGGATAA
AGTTGCAGGA
CCACTTCTGC
GCTCGGCCCT
TCCGGCTGGC
TGGTTTATTG
CTGATAAATC
TGGAGCCGGT
GAGCGTGGGT
CTCGCGGTAT
CATTGCAGCA
CTGGGGCCAG
ATGGTAAGCC
CTCCCGTATC
GTAGTTATCT
ACACGACGGG
GAGTCAGGCA
ACTATGGATG
AACGAAATAG
ACAGATCGCT
GAGATAGGTG
CCTCACTGAT
TAAGCATTGG
TAACTGTCAG
ACCAAGTTTA
CTCATATATA
CTTTAGATTG
ATTTAAAACT
TCATTTTTAA
TTTAAAAGGA
TCTAGGTGAA
GATCCTTTTT
GATAATCTCA
TGAGCGGATA
CATATTTGAA
TGTATTTAGA
AAAATAAACA
AATAGGGGTT
CCGCGCACAT
TTCCCCGAAA
AGTGCCACCT
GATGCGGTGT
GAAATACCGC
ACAGATGCGT
AAGGAGAAAA
TACCGCATCA
GGAAATTGTA
AGCGTTAATA
ATTCAGAAGA
ACTCGTCAAG
AAGGCGATAG
AAGGCGATGC
GCTGCGAATC
GGGAGCGGCG
ATACCGTAAA
GCACGAGGAA
GCGGTCAGCC
CATTCGCCGC
CAAGCTCTTC
AGCAATATCA
CGGGTAGCCA
ACGCTATGTC
CTGATAGCGG
TCCGCCACAC
CCAGCCGGCC
ACAGTCGATG
AATCCAGAAA
AGCGGCCATT
TTCCACCATG
ATATTCGGCA
AGCAGGCATC
GCCATGCATG
GGTCACGACG
AGATCCTCGC
CGTCGGGCAT
GCTCGCCTTG
AGCCTGGCGA
ACAGTTCGGC
TGGCGCGAGC
CCCTGATGCT
CTTCGTCCAG
ATCATCCTGA
TCGACAAGAC
CGGCTTCCAT
CCGAGTACGT
GCTCGCTCGA
TGCGATGTTT
CGCTTGGTGG
TCGAATGGGC
AGGTAGCCGG
ATCAAGCGTA
TGCAGCCGCC
GCATTGCATC
AGCCATGATG
GATACTTTCT
CGGCAGGAGC
AAGGTGAGAT
GACAGGAGAT
CCTGCCCCGG
CACTTCGCCC
AATAGCAGCC
AGTCCCTTCC
CGCTTCAGTG
ACAACGTCGA
GCACAGCTGC
GCAAGGAACG
CCCGTCGTGG
CCAGCCACGA
TAGCCGCGCT
GCCTCGTCTT
GCAGTTCATT
CAGGGCACCG
GACAGGTCGG
TCTTGACAAA
AAGAACCGGG
CGCCCCTGCG
CTGACAGCCG
GAACACGGCG
GCATCAGAGC
AGCCGATTGT
CTGTTGTGCC
CAGTCATAGC
CGAATAGCCT
CTCCACCCAA
GCGGCCGGAG
AACCTGCGTG
CAATCCATCT
TGTTCAATCA
TGCGAAACGA
TCCTCATCCT
GTCTCTTGAT
CAGAGCTTGA
TCCCCTGCGC
CATCAGATCC
TTGGCGGCAA
GAAAGCCATC
CAGTTTACTT
TGCAGGGCTT
CCCAACCTTA
CCAGAGGGCG
CCCCAGCTGG
CAATTCCGGT
TCGCTTGCTG
TCCATAAAAC
CGCCCAGTCT
AGCTATCGCC
ATGTAAGCCC
ACTGCAAGCT
ACCTGCTTTC
TCTTTGCGCT
TGCGTTTTCC
CTTGTCCAGA
TAGCCCAGTA
GCTGACATTC
ATCCGGGGTC
AGCACCGTTT
CTGCGGACTG
GCTTTCTACG
TGAAAAGGAT
CTAGGTGAAG
ATCCTTTTTG
ATAATCTCAT
GCCTGACATT
TATATTCCCC
AGAACATCAG
GTTAATGGCG
TTTTTGATGT
CATTTTCGCG
GTGGCTGAGA
TCAGCCACTT
CTTCCCCGAT
AACGGAGACC
GGCACACTGG
CCATATCGGT
GGTCATCATG
CGCCAGCTTT
CATCCCCGAT
ATGCACCACC
GGGTAAAGTT
CACGGGAGAC
TTTATCTGAC
AGCAGACGTG
CACTGGCCAG
GGGGATCACC
ATCCGTCGCC
CCGGCGTGTC
AATAATATCA
CTCTGTACAT
CCACAAACAG
ACGATAACGG
CTCTCTCTTT
TATAGGTGTA
AACCTTAAAC
TGCCGTACGT
ATAGGCTGCG
CAACTGTTGG
GAAGGGCGAT
CGGTGCGGGC
CTCTTCGCTA
TTACGCCAGC
TGGCGAAAGG
GGGATGTGCT
GCAAGGCGAT
TAAGTTGGGT
AACGCCAGGG
TTTTCCCAGT
CACGACGTTG
TAAAACGACG
GCCAGTGAAT
TGTAATACGA
CTCACTATAG
GGCGAATTGA
ATTTAGCGGC
CGCGAATTCG
CCCTTAGAGC
AGAGCCAGGA
ACCCCTGTAG
GGAAGGGGCA
GGAGAGCCAG
GGGCATGAGA
TGGTGGACGA
GGAAGGGGGA
CAGGGAAGCC
TGAGCGCCTC
TCCTGGGCTT
GCCAAGGACT
CAAACCCAGA
AGCCCAGAGC
AGGGCCTTAG
GGAAGCGGGA
CCCTGCTCTG
GGCGGAGGAA
TATGTCCCAG
ATAGCACTGG
GGACTCTTTA
AGGAAAGAAG
GATGGAGAAA
GAGAAAGGGA
GTAGAGGCGG
CCACGACCTG
GTGAACACCT
AGGACGCACC
ATTCTCACAA
AGGGAGTTTT
CCACACGGAC
ACCCCCCTCC
TCACCACAGC
CCTGCCAGGA
CGGGGCTGGC
TACTGGCCTT
ATCTCACAGG
TAAAACTGAC
GCACGGAGGA
ACAATATAAA
TTGGGGACTA
GAAAGGTGAA
GAGCCAAAGT
TAGAACTCAG
GACCAACTTA
TTCTGATTTT
GTTTTTCCAA
ACTGCTTCTC
CTCTTGGGAA
GTGTAAGGAA
GCTGCAGCAC
CAGGATCAGT
GAAACGCACC
AGACAGCCGC
GTCAGAGCAG
CTCAGGTTCT
GGGAGAGGGT
AGCGCAGGGT
GGCCACTGAG
AACCGGGCAG
GTCACGCATC
CCCCCCTTCC
CTCCCACCCC
CTGCCAAGCT
CTCCCTCCCA
GGATCCTCTC
TGGCTCCATC
GTAAGCAAAC
CTTAGAGGTT
CTGGCAAGGA
GAGAGATGGC
TCCAGGAAAT
GGGGGTGTGT
CACCAGATAA
GGAATCTGCC
TAACAGGAGG
TGGGGGTTAG
ACCCAATATC
AGGAGACTAG
GAAGGAGGAG
GCCTAAGGAT
GGGGCTTTTC
TGTCACCAAT
CCTGTCCCTA
GTAAAGCTTA
GTACTGTCGA
GTTTACTCCC
TATCAGTGAT
AGAGAACGTA
TGTCGAGTTT
ACTCCCTATC
AGTGATAGAG
AACGATGTCG
AGTTTACTCC
CTATCAGTGA
TAGAGAACGT
ATGTCGAGTT
TACTCCCTAT
CAGTGATAGA
GAACGTATGT
CGAGTTTACT
CCCTATCAGT
GATAGAGAAC
GTATGTCGAG
TTTATCCCTA
TCAGTGATAG
AGAACGTATG
TCGAGTTTAC
TCCCTATCAG
TGATAGAGAA
CGTATGTCGA
GGTAGGCGTG
TACGGTGGGA
GGCCTATATA
AGCAGAGCTC
GTTTAGTGAA
CCGTCAGATC
GCCTGGAGAA
TTCCGATCAA
CAAGTTTGTA
CAAAAAAGCA
GGCTCCGCGG
TTTTCAGGTT
GGAATGCCAA
AGAAGAAGCG
GAAGGTCGGT
GGCGGCTCAC
CCGGGATGGA
CAAGAAGTAC
TCCATTGGCC
TCGCCATCGG
AACAAATAGC
GTGGGCTGGG
CTGTCATCAC
AGATGAGTAC
AAGGTGCCTA
GCAAGAAATT
TAAGGTGCTG
GGAAATACAG
ACAGACATAG
CATCAAGAAG
AACCTCATTG
GCGCTCTCCT
GTTTGACTCC
GGCGAAACAG
CCGAAGCTAC
CAGACTCAAG
AGAACCGCTA
GGAGAAGGTA
CACCAGAAGG
AAAAACAGGA
TTTGCTACCT
GCAGGAAATT
TTTTCCAACG
AGATGGCCAA
GGTGGACGAT
TCCTTCTTCC
ATAGGCTGGA
AGAGAGCTTC
CTCGTGGAGG
AAGACAAGAA
ACACGAGAGG
CATCCTATTT
TTGGCAATAT
TGTGGATGAG
GTCGCCTACC
ATGAGAAGTA
TCCCACAATC
TATCATCTGA
GAAAAAAACT
GGTGGATAGC
ACCGACAAGG
CCGATCTCAG
GCTCATTTAT
CTCGCTCTGG
CTCACATGAT
CAAGTTTAGG
GGCCACTTCC
TGATCGAAGG
CGACCTGAAT
CCCGACAACT
CCGACGTGGA
CAAACTGTTC
ATCCAGCTCG
TCCAGACCTA
CAATCAACTC
TTCGAGGAGA
ACCCCATCAA
TGCTTCCGGC
GTGGATGCCA
AGGCCATCCT
GAGCGCTAGG
CTCTCCAAGT
CCAGGAGGCT
GGAAAATCTG
ATCGCCCAAC
TCCCTGGAGA
GAAGAAGAAC
GGCCTGTTTG
GCAATCTGAT
TGCCCTGAGC
CTCGGACTCA
CCCCCAACTT
CAAGAGCAAC
TTCGATCTCG
CCGAAGACGC
CAAACTCCAA
CTGAGCAAGG
ATACCTACGA
CGACGATCTC
GATAATCTCC
TCGCCCAGAT
CGGCGATCAA
TATGCCGACC
TCTTTCTGGC
CGCCAAAAAC
CTGAGCGACG
CTATTCTGCT
CAGCGACATT
CTCAGGGTGA
ATACAGAAAT
CACAAAAGCC
CCCCTGTCCG
CCAGCATGAT
CAAAAGGTAC
GATGAACACC
ATCAGGACCT
CACCCTGCTG
AAGGCTCTGG
TCAGGCAGCA
ACTCCCCGAA
AAGTACAAGG
AGATTTTCTT
TGATCAGTCC
AAGAATGGAT
ATGCTGGCTA
TATTGATGGA
GGCGCCTCCC
AGGAGGAATT
TTATAAATTC
ATCAAGCCCA
TTCTCGAAAA
GATGGACGGA
ACCGAAGAGC
TGCTGGTCAA
ACTCAATAGG
GAGGATCTGC
TGAGGAAGCA
AAGGACCTTC
GACAATGGCA
GCATCCCCCA
CCAGATCCAC
CTCGGCGAAC
TCCACGCTAT
CCTCAGGAGG
CAGGAAGACT
TCTACCCTTT
CCTGAAGGAT
AACAGGGAGA
AAATCGAGAA
AATCCTGACC
TTCAGAATCC
CCTACTACGT
CGGACCTCTC
GCCAGGGGCA
ATTCCAGATT
CGCCTGGATG
ACAAGGAAGA
GCGAGGAAAC
AATCACACCA
TGGAACTTCG
AAGAAGTGGT
CGATAAGGGC
GCCAGCGCCC
AGAGCTTCAT
TGAAAGGATG
ACCAACTTTG
ATAAGAACCT
GCCCAATGAG
AAGGTGCTGC
CTAAGCACTC
CCTGCTGTAT
GAGTATTTCA
CCGTGTATAA
TGAGCTGACC
AAGGTCAAGT
ACGTCACCGA
GGGAATGAGA
AAGCCTGCTT
TTCTCTCCGG
CGAGCAGAAA
AAAGCCATCG
TGGACCTGCT
GTTCAAGACC
AACAGGAAGG
TGACCGTCAA
GCAACTCAAG
GAGGACTACT
TTAAGAAGAT
TGAGTGCTTT
GATAGCGTGG
AAATTAGCGG
AGTCGAGGAC
AGGTTCAATG
CCTCCCTCGG
AACATATCAC
GACCTGCTGA
AAATCATCAA
AGACAAAGAT
TTTCTGGATA
ACGAGGAGAA
TGAAGACATT
CTGGAGGACA
TTGTCCTCAC
CCTGACCCTG
TTTGAGGACA
GAGAGATGAT
TGAAGAGAGG
CTGAAAACCT
ATGCCCACCT
GTTCGACGAC
AAGGTGATGA
AGCAGCTCAA
AAGAAGGAGA
TATACCGGCT
GGGGCAGACT
GTCCAGGAAG
CTGATCAACG
GCATTAGGGA
CAAGCAGAGC
GGCAAGACCA
TTCTCGACTT
TCTCAAGTCC
GACGGATTCG
CCAACAGAAA
CTTTATGCAA
CTGATCCACG
ATGACAGCCT
CACCTTTAAG
GAAGATATTC
AGAAGGCTCA
GGTCAGCGGC
CAAGGCGATT
CCCTCCATGA
GCACATCGCT
AATCTGGCTG
GCTCCCCTGC
TATCAAAAAG
GGCATCCTCC
AGACAGTCAA
AGTCGTCGAT
GAGCTGGTCA
AGGTGATGGG
CAGGCATAAA
CCCGAGAACA
TTGTGATTGA
GATGGCTAGG
GAGAACCAGA
CCACCCAGAA
AGGCCAGAAA
AACAGCAGAG
AAAGAATGAA
GAGGATCGAG
GAGGGCATCA
AAGAACTGGG
CAGCCAAATC
CTCAAGGAGC
ACCCCGTCGA
AAATACACAA
CTCCAGAACG
AAAAACTCTA
CCTCTACTAT
CTGCAGAACG
GCAGAGACAT
GTACGTGGAC
CAGGAACTGG
ACATCAACAG
GCTCTCCGAT
TACGATGTGG
ACGCCATCGT
CCCTCAGTCC
TTTCTGAAAG
ATGATAGCAT
CGACAACAAG
GTGCTGACCA
GGTCCGACAA
GAATAGGGGC
AAGAGCGATA
ATGTGCCCTC
CGAGGAGGTC
GTCAAAAAAA
TGAAAAACTA
CTGGAGACAA
CTCCTCAACG
CTAAGCTCAT
CACCCAAAGA
AAGTTCGACA
ATCTGACCAA
AGCCGAGAGG
GGCGGCCTCT
CCGAACTGGA
CAAGGCCGGC
TTCATCAAAA
GGCAATTGGT
GGAAACCAGG
CAGATTACAA
AGCATGTCGC
TCAAATTCTC
GATAGCAGGA
TGAATACCAA
ATATGACGAG
AACGACAAGC
TGATCAGAGA
GGTCAAGGTC
ATCACACTCA
AGTCCAAGCT
CGTGAGCGAC
TTCAGAAAAG
ATTTCCAATT
TTATAAAGTC
AGGGAGATCA
ACAATTACCA
CCACGCTCAC
GACGCTTATC
TCAACGCTGT
CGTGGGAACC
GCCCTGATCA
AAAAATACCC
CAAGCTGGAA
AGCGAGTTCG
TGTATGGCGA
TTATAAAGTG
TACGACGTGA
GGAAGATGAT
CGCTAAAAGC
GAGCAGGAAA
TCGGCAAGGC
TACAGCCAAG
TACTTTTTCT
ACTCCAACAT
TATGAACTTC
TTCAAGACCG
AGATTACCCT
CGCCAACGGC
GAAATTAGGA
AGAGGCCCCT
GATTGAAACA
AATGGAGAAA
CAGGCGAAAT
CGTCTGGGAC
AAGGGCAGGG
ACTTCGCCAC
AGTCAGAAAA
GTGCTGTCCA
TGCCTCAAGT
CAACATCGTC
AAAAAGACCG
AGGTGCAGAC
CGGCGGCTTT
AGCAAAGAAA
GCATCCTGCC
CAAGAGAAAC
TCCGACAAGC
TCATCGCTAG
GAAGAAGGAC
TGGGACCCTA
AGAAATACGG
AGGATTTGAC
TCCCCTACCG
TCGCCTATTC
CGTCCTCGTC
GTCGCTAAGG
TGGAGAAGGG
CAAGAGCAAG
AAGCTCAAGA
GCGTCAAGGA
GCTGCTGGGA
ATCACCATCA
TGGAGAGGAG
CTCCTTCGAA
AAAAACCCTA
TTGATTTCCT
GGAGGCCAAG
GGCTACAAGG
AGGTCAAGAA
GGACCTCATC
ATCAAGCTGC
CCAAATACAG
CCTCTTCGAA
CTGGAAAATG
GCAGGAAGAG
AATGCTCGCT
AGCGCCGGCG
AGCTCCAGAA
AGGAAATGAG
CTGGCTCTGC
CCAGCAAGTA
CGTCAACTTC
CTCTATCTCG
CCAGCCACTA
TGAAAAGCTC
AAGGGCAGCC
CCGAAGACAA
TGAGCAGAAG
CAGCTCTTCG
TCGAGCAGCA
CAAGCACTAC
CTCGATGAAA
TCATCGAGCA
AATCAGCGAG
TTTTCCAAAA
GGGTGATTCT
CGCCGACGCT
AACCTCGATA
AGGTCCTCTC
CGCTTACAAC
AAGCATAGAG
ACAAGCCCAT
CAGAGAACAG
GCCGAGAACA
TCATCCACCT
GTTTACACTC
ACAAACCTCG
GAGCCCCTGC
CGCTTTTAAA
TACTTCGATA
CAACCATTGA
TAGGAAGAGG
TACACCTCCA
CCAAGGAGGT
GCTGGATGCT
ACCCTGATTC
ATCAATCCAT
CACAGGACTC
TACGAAACAA
GGATTGACCT
GTCCCAACTG
GGAGGCGACA
CTAGTAGAGC
TGACCCCAAG
AAGAAGAGGA
AGGTGGAGGC
CAGCGGTTCC
GGACGGGCTG
ACGCATTGGA
CGATTTTGAT
CTGGATATGC
TGGGAAGTGA
CGCCCTCGAT
GATTTTGACC
TTGACATGCT
TGGTTCGGAT
GCCCTTGATG
ACTTTGACCT
CGACATGCTC
GGCAGTGACG
CCCTTGATGA
TTTCGACCTG
GACATGCTGA
TTAACTCTAG
AAGCCAATAC
CTCCCAGACA
CAGACGACAG
GCATAGGATT
GAGGAAAAAA
GAAAGAGGAC
GTATGAAACT
TTCAAGAGCA
TAATGAAAAA
AAGTCCGTTC
TCTGGTCCTA
CAGACCCCAG
GCCCCCACCT
AGACGAATCG
CGGTTCCGAG
CCGATCATCA
GCGAGTGTTC
CTAAGCCAGC
TCCACAGCCC
TATCCATTTA
CGTCTAGTCT
GTCTACGATC
AACTACGACG
AGTTCCCCAC
TATGGTATTC
CCGTCAGGCC
AGATAAGCCA
AGCTAGTGCG
TTGGCACCCG
CACCCCCACA
GGTGCTCCCA
CAAGCTCCCG
CTCCTGCGCC
AGCACCAGCT
ATGGTTAGTG
CCCTCGCGCA
AGCGCCTGCC
CCGGTTCCTG
TTCTCGCACC
TGGGCCACCG
CAGGCAGTTG
CACCGCCAGC
GCCAAAGCCC
ACACAGGCAG
GGGAAGGTAC
GTTGAGCGAA
GCCCTTCTGC
AACTCCAATT
TGACGATGAG
GACCTCGGAG
CACTTCTTGG
CAATTCAACG
GACCCCGCAG
TATTTACTGA
TCTTGCCAGC
GTGGACAATT
CCGAATTTCA
ACAGCTCCTG
AACCAGGGAA
TCCCGGTGGC
TCCCCATACG
ACAGAGCCCA
TGCTCATGGA
GTACCCGGAG
GCCATTACAC
GCCTTGTGAC
GGGCGCACAG
CGACCCCCTG
ACCCGGCACC
GGCTCCCTTG
GGGGCTCCTG
GGCTTCCCAA
CGGGTTGCTG
TCAGGCGACG
AGGACTTTAG
CTCAATCGCC
GACATGGATT
TTAGCGCCCT
GTTGGGTAGC
GGAAGTGGTA
GCCGCGACAG
CCGGGAAGGC
ATGTTCCTTC
CGAAACCGGA
AGCTGGTAGC
GCCATAAGCG
ACGTATTTGA
GGGTAGGGAG
GTGTGTCAGC
CTAAGCGGAT
TCGACCATTC
CACCCCCCTG
GCTCTCCTTG
GGCAAATCGA
CCCCTCCCGG
CGAGCCTTGC
CCCTACGCCG
ACCGGACCCG
TGCATGAGCC
CGTCGGTAGC
CTCACGCCAG
CCCCCGTGCC
GCAACCTCTC
GACCCTGCTC
CGGCGGTTAC
GCCAGAAGCA
AGCCACCTTT
TGGAGGACCC
GGACGAAGAA
ACAAGCCAGG
CGGTAAAGGC
GCTCCGCGAA
ATGGCCGACA
CCGTGATACC
CCAGAAGGAG
GAAGCCGCCA
TTTGCGGCCA
AATGGACCTT
TCCCACCCTC
CACCACGCGG
CCACCTGGAC
GAGCTGACGA
CCACCCTGGA
AAGCATGACC
GAGGATTTGA
ATCTGGACAG
CCCGTTGACC
CCCGAATTGA
ACGAAATCCT
GGACACGTTC
CTGAATGATG
AGTGTTTGTT
GCACGCTATG
CACATCAGCA
CCGGGTTTAG
TATATTTGAC
ACAAGTTTGT
TTTAACTCGA
GACTAGATAA
CTGATCGGCG
CGCCGACCCA
GCTTTCTTGT
ACAAAGTGGT
TGATCCGGGG
ATCCTCTAGT
CAGCTGACGC
GTGCTAGCGC
GGCCGCATCG
ATAAGCTTGT
CGACGATATC
TCTAGAGGAT
CATAATCAGC
CATACCACAT
TTGTAGAGGT
TTTACTTGCT
TTAAAAAACC
TCCCACACCT
CCCCCTGAAC
CTGAAACATA
AAATGAATGC
AATTGTTGTT
GTTAACTTGT
TTATTGCAGC
TTATAATGGT
TACAAATAAA
GCAATAGCAT
CACAAATTTC
ACAAATAAAG
CATTTTTTTC
ACTGCCTCGA
CCCATAGAGC
CCACCGCATC
CCCAGCATGC
CTGCTATTGT
CTTCCCAATC
CTCCCCCTTG
CTGTCCTGCC
CCACCCCACC
CCCCAGAATA
GAATGACACC
TACTCAGACA
ATGCGATGCA
ATTTCCTCAT
TTTATTAGGA
AAGGACAGTG
GGAGTGGCAC
CTTCCAGGGT
CAAGGAAGGC
ACGGGGGAGG
GGCAAACAAC
AGATGGCTGG
CAACTAGAAG
GCACAGTCGA
GGCTGATCAG
CGGGTTTAAA
CGGGCCCTCT
AGATCAGGCA
CCGGGCTTGC
GGGTCATGCA
CCAGGTGCGC
GGTCCTTCGG
GCACCTCGAC
GTCGGCGGTG
ACGGTGAAGC
CGAGCCGCTC
GTAGAAGGGG
AGGTTGCGGG
GCGCGGAGGT
CTCCAGGAAG
GCGGGCACCC
CGGCGCGCTC
GGCCGCCTCC