名称: | pLV2-TRE3GS-EGFP-TetOne-Puro |
---|---|
别称: | - |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达,四环素诱导 |
片段类型: | ORF |
片段物种: | |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 绿色 |
启动子: | TRE3GS |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACTACTAAGT
TTGTAGTACA
TATTTAACAA
ATACAATTTC
TTTAAAAACT
ACTAAGTTTG
TAGTACATAT
TTAACAAATA
CAATTTCTTT
AAAATGAAAA
TAATTCAGAG
GAATCACAGG
TTTAGAGTAA
ATGAAACCAC
TAGGTAATTG
GCAGTGGTAA
TAGGGTATGG
GGTGGGAAGT
TTGGGATGAT
TTTGGTTAGC
TTGAGTTATC
CAGTTGATCC
AGACATGATA
AGATACATTG
ATGAGTTTGG
ACAAACCACA
ACTAGAATGC
AGTGAAAAAA
ATGCTTTATT
TGTGAAATTT
GTGATGCTAT
TGCTTTATTT
GTAACCATTA
TAAGCTGCAA
TAAACAAGTT
GATCTCCCGA
TCCGTCGACG
TCAGGTGGCA
CTTTTCGGGG
AAATGTGCGC
GGAACCCCTA
TTTGTTTATT
TTTCTAAATA
CATTCAAATA
TGTATCCGCT
CATGAGACAA
TAACCCTGAT
AAATGCTTCA
ATAATATTGA
AAAAGGAAGA
GTATGAGTAT
TCAACATTTC
CGTGTCGCCC
TTATTCCCTT
TTTTGCGGCA
TTTTGCCTTC
CTGTTTTTGC
TCACCCAGAA
ACGCTGGTGA
AAGTAAAAGA
TGCTGAAGAT
CAGTTGGGTG
CACGAGTGGG
TTACATCGAA
CTGGATCTCA
ACAGCGGTAA
GATCCTTGAG
AGTTTTCGCC
CCGAAGAACG
TTTTCCAATG
ATGAGCACTT
TTAAAGTTCT
GCTATGTGGC
GCGGTATTAT
CCCGTATTGA
CGCCGGGCAA
GAGCAACTCG
GTCGCCGCAT
ACACTATTCT
CAGAATGACT
TGGTTGAGTA
CTCACCAGTC
ACAGAAAAGC
ATCTTACGGA
TGGCATGACA
GTAAGAGAAT
TATGCAGTGC
TGCCATAACC
ATGAGTGATA
ACACTGCGGC
CAACTTACTT
CTGACAACGA
TCGGAGGACC
GAAGGAGCTA
ACCGCTTTTT
TGCACAACAT
GGGGGATCAT
GTAACTCGCC
TTGATCGTTG
GGAACCGGAG
CTGAATGAAG
CCATACCAAA
CGACGAGCGT
GACACCACGA
TGCCTGTAGC
AATGGCAACA
ACGTTGCGCA
AACTATTAAC
TGGCGAACTA
CTTACTCTAG
CTTCCCGGCA
ACAATTAATA
GACTGGATGG
AGGCGGATAA
AGTTGCAGGA
CCACTTCTGC
GCTCGGCCCT
TCCGGCTGGC
TGGTTTATTG
CTGATAAATC
TGGAGCCGGT
GAGCGTGGGT
CTCGCGGTAT
CATTGCAGCA
CTGGGGCCAG
ATGGTAAGCC
CTCCCGTATC
GTAGTTATCT
ACACGACGGG
GAGTCAGGCA
ACTATGGATG
AACGAAATAG
ACAGATCGCT
GAGATAGGTG
CCTCACTGAT
TAAGCATTGG
TAACTGTCAG
ACCAAGTTTA
CTCATATATA
CTTTAGATTG
ATTTAAAACT
TCATTTTTAA
TTTAAAAGGA
TCTAGGTGAA
GATCCTTTTT
GATAATCTCA
TGACCAAAAT
CCCTTAACGT
GAGTTTTCGT
TCCACTGAGC
GTCAGACCCC
GTAGAAAAGA
TCAAAGGATC
TTCTTGAGAT
CCTTTTTTTC
TGCGCGTAAT
CTGCTGCTTG
CAAACAAAAA
AACCGCTACC
AGCGGTGGTT
TGTTTGCCGG
ATCAAGAGCT
ACCAACTCTT
TTTCCGAAGG
TAACTGGCTT
CAGCAGAGCG
CAGATACCAA
ATACTGTTCT
TCTAGTGTAG
CCGTAGTTAG
GCCACCACTT
CAAGAACTCT
GTAGCACCGC
CTACATACCT
CGCTCTGCTA
ATCCTGTTAC
CAGTGGCTGC
TGCCAGTGGC
GATAAGTCGT
GTCTTACCGG
GTTGGACTCA
AGACGATAGT
TACCGGATAA
GGCGCAGCGG
TCGGGCTGAA
CGGGGGGTTC
GTGCACACAG
CCCAGCTTGG
AGCGAACGAC
CTACACCGAA
CTGAGATACC
TACAGCGTGA
GCTATGAGAA
AGCGCCACGC
TTCCCGAAGG
GAGAAAGGCG
GACAGGTATC
CGGTAAGCGG
CAGGGTCGGA
ACAGGAGAGC
GCACGAGGGA
GCTTCCAGGG
GGAAACGCCT
GGTATCTTTA
TAGTCCTGTC
GGGTTTCGCC
ACCTCTGACT
TGAGCGTCGA
TTTTTGTGAT
GCTCGTCAGG
GGGGCGGAGC
CTATGGAAAA
ACGCCAGCAA
CGCGGCCTTT
TTACGGTTCC
TGGCCTTTTG
CTGGCCTTTT
GCTCACATGT
TCTTTCCTGC
GTTATCCCCT
GATTCTGTGG
ATAACCGTAT
TACCGCCTTT
GAGTGAGCTG
ATACCGCTCG
CCGCAGCCGA
ACGACCGAGC
GCAGCGAGTC
AGTGAGCGAG
GAAGCGGAAG
AGCGCCCAAT
ACGCAAACCG
CCTCTCCCCG
CGCGTTGGCC
GATTCATTAA
TGCAGCTGGC
ACGACAGGTT
TCCCGACTGG
AAAGCGGGCA
GTGAGCGCAA
CGCAATTAAT
GTGAGTTAGC
TCACTCATTA
GGCACCCCAG
GCTTTACACT
TTATGCTTCC
GGCTCGTATG
TTGTGTGGAA
TTGTGAGCGG
ATAACAATTT
CACACAGGAA
ACAGCTATGA
CCATGATTAC
GCCAAGCTCT
AGCTAGAGGT
CGACGGTAAA
ACGCTAGCAA
TGTCAAGGCC
TCTCACTCTC
TGATATTCAT
TTCTTTGCAA
GTTATAAATA
CTGAATAATA
AGATGACATG
AACTACTACT
GCTAGAGATT
TTCCACACTG
ACTAAAAGGG
TCTGAGGGAT
CTCTAGTTAC
CAGAGTCACA
CAACAGACGG
GCACACACTA
CTTGAAGCAC
TCAAGGCAAG
CTTTATTGAG
GCTTAAGCAG
TGGGTTCCCT
AGTTAGCCAG
AGAGCTCCCA
GGCTCAGATC
TGGTCTAACC
AGAGAGACCC
AGTACAGGCA
AAAAGCAGCT
GCTTATATGC
AGGATCTGAG
GGCTCGCCAC
TCCCCAGTCC
CGCCCAGGCC
ACGCCTCCCT
GGAAAGTCCC
CAGCGGAAAG
TCCCTTGTAG
CAAGCTCGAT
ATCAGCAGTT
CTTGAAGTAC
TCCGGATGCA
GCTCTCGGGC
CACGTGATGA
AATGCTAGGC
GGCTGTCAAA
CCTCCACTCT
AACACTTCTC
TCTCCGGGTC
ATCCATCCCA
TGCAGGCTCA
CAGGGTGTAA
CAAGCTGGTG
TTCTCTCCTT
TATTGGCCTC
TTCTACCTTA
TCTGGCTCAA
CTGGTACTAG
CTTGTAGCAC
CATCCAAAGG
TCAGTGGATA
TCTGACCCCT
GGCCCTGGTG
TGTAGTTCTG
CTAATCAGGG
AAGTAGCCTT
GTGTGTGGTA
GATCCACAGA
TCAAGGATAT
CTTGTCTTCG
TTGGGAGTGA
ATTAGCCCTT
CCAGTCCCCT
CTTTTCTTTT
AAAAAGTGGC
TAAGATCTAC
AGCTGCCTTG
TAAGTCATTG
GTCTTAAAGG
TACCTGAGGT
GTGACTGGAA
AACCCACCTC
CTCCTCCTCT
TGTGCTTCTA
GCCAGGCACA
ATCAGCATTG
GTAGCTGCTG
TATTGCTACT
TGTGATTGCT
CCATGTTTTT
CTAGGTCTCG
ACTGCAGAAT
TAATTCCAGG
CGGGGAGGCG
GCCCAAAGGG
AGATCCGACT
CGTCTGAGGG
CGAAGGCGAA
GACGCGGAAG
AGGCCGCAGA
GCCGGCAGCA
GGCCGCGGGA
AGGAAGGTCC
GCTGGATTGA
GGGCCGAAGG
GACGTAGCAG
AAGGACGTCC
CGCGCAGAAT
CCAGGTGGCA
ACACAGGCGA
GCAGCCATGG
AAAGGACGTC
AGCTTCCCCG
ACAACACCAC
GGAATTGTCA
GTGCCCAACA
GCCGAGCCCC
TGTCCAGCAG
CGGGCAAGGC
AGGCGGCGAT
GAGTTCCGCC
GTGGCAATAG
GGAGGGGGAA
AGCGAAAGTC
CCGGAAAGGA
GCTGACAGGT
GGTGGCAATG
CCCCAACCAG
TGGGGGTTGC
GTCAGCAAAC
ACAGTGCACA
CCACGCCACG
TTGCCTGACA
ACGGGCCACA
ACTCCTCATA
AAGAGACAGC
AACCAGGATT
TATACAAGGA
GGAGAAAATG
AAAGCCATAC
GGGAAGCAAT
AGCATGATAC
AAAGGCATTA
AAGCAGCGTA
TCCACATAGC
GTAAAAGGAG
CAACATAGTT
AAGAATACCA
GTCAATCTTT
CACAAATTTT
GTAATCCAGA
GGTTGATTGT
TCCAGACGCG
TTCAGGCACC
GGGCTTGCGG
GTCATGCACC
AGGTGCGCGG
TCCTTCGGGC
ACCTCGACGT
CGGCGGTGAC
GGTGAAGCCG
AGCCGCTCGT
AGAAGGGGAG
GTTGCGGGGC
GCGGAGGTCT
CCAGGAAGGC
GGGCACCCCG
GCGCGCTCGG
CCGCCTCCAC
TCCGGGGAGC
ACGACGGCGC
TGCCCAGACC
CTTGCCCTGG
TGGTCGGGCG
AGACGCCGAC
GGTGGCCAGG
AACCACGCGG
GCTCCTTGGG
CCGGTGCGGC
GCCAGGAGGC
CTTCCATCTG
TTGCTGCGCG
GCCAGCCGGG
AACCGCTCAA
CTCGGCCATG
CGCGGGCCGA
TCTCGGCGAA
CACCGCCCCC
GCTTCGACGC
TCTCCGGCGT
GGTCCAGACC
GCCACCGCGG
CGCCGTCGTC
CGCGACCCAC
ACCTTGCCGA
TGTCGAGCCC
GACGCGCGTG
AGGAAGAGTT
CTTGCAGCTC
GGTGACCCGC
TCGATGTGGC
GGTCCGGGTC
GACGGTGTGG
CGCGTGGCGG
GGTAGTCGGC
GAACGCGGCG
GCGAGGGTGC
GTACGGCCCG
GGGGACGTCG
TCGCGGGTGG
CGAGGCGCAC
CGTGGGCTTG
TACTCGGTCA
TGGTCGCGTT
TTGCAAAAGC
CTAGGCCTCC
AAAAAAGCCT
CCTCACTACT
TCTGGAATAG
CTCAGAGGCC
GAGGCGGCCT
CGGCCTCTGC
ATAAATAAAA
AAAATTAGTC
AGCCATGGGG
CGGAGAATGG
GCGGAACTGG
GCGGAGTTAG
GGGCGGGATG
GGCGGAGTTA
GGGGCGGGAC
TATGGTTGCT
GACTAATTGA
GATGCATGCT
TTGCATACTT
CTGCCTGCTG
GGGAGCCTGG
GGACTTTCCA
CACCTGGTTG
CTGACTAATT
GAGATGCATG
CTTTGCATAC
TTCTGCCTGC
TGGGGAGCCT
GGGGACTTTC
CACACCCTAA
CTGACACACA
TTCGCGCGTT
TACCCGGGGA
GCATGTCAAG
GTCAAAATCG
TCAAGAGCGT
CAGCAGGCAG
CATATCAAGG
TCAAAGTCGT
CAAGGGCATC
GGCTGGGAGC
ATGTCTAAGT
CAAAATCGTC
AAGGGCGTCG
GTCGGCCCGC
CGCTTTCGCA
CTTTAGCTGT
TTCTCCAGGC
CACATATGAT
TAGTTCCAGG
CCGAAAAGGA
AGGCAGGTTC
GGCTCCCTGC
CGGTCGAACA
GCTCAATTGC
TTGTTTCAGA
AGTGGGGGCA
TAGAATCGGT
GGTAGGTGTC
TCTCTTTCCT
CTTTTGCTAC
TTGATGCTCC
TGTTCCTCCA
ATACGCAGCC
CAGTGTAAAG
TGGCCCACGG
CGGACAGAGC
GTACAGTGCG
TTCTCCAGGG
AGAAGCCTTG
CTGACACAGG
AACGCGAGCT
GATTTTCCAG
GGTTTCGTAC
TGTTTCTCTG
TTGGGCGGGT
GCCGAGATGC
ACTTTAGCCC
CGTCGCGATG
TGAGAGGAGA
GCACAGCGGT
ATGACTTGGC
GTTGTTCCGC
AGAAAGTCTT
GCCATGACTC
GCCTTCCAGG
GGGCAGGAGT
GGGTATGATG
CCTGTCCAGC
ATCTCGATTG
GCAGGGCATC
GAGCAGGGCC
CGCTTGTTCT
TCACGTGCCA
GTACAGGGTA
GGCTGCTCAA
CTCCCAGCTT
TTGAGCGAGT
TTCCTTGTCG
TCAGGCCTTC
GATACCGACT
CCATTGAGTA
ATTCCAGAGC
AGAGTTTATG
ACTTTGCTCT
TGTCCAGTCT
AGACATGGTA
ATTCGATGAT
CCCCCTGGGG
AGAGAGGTCG
GTGATTCGGT
CAACGAGGGA
GCCGACTGCC
GACGTGCGCT
CCGGAGGCTT
GCAGAATGCG
GAACACCGCG
CGGGCAGGAA
CAGGGCCCAC
ACTACCGCCC
CACACCCCGC
CTCCCGCACC
GCCCCTTCCC
GGCCGCTGCT
CTCGGCGCGC
CCTGCTGAGC
AGCCGCTATT
GGCCACAGCC
CATCGCGGTC
GGCGCGCTGC
CATTGCTCCC
TGGCGCTGTC
CGTCTGCGAG
GGTACTAGTG
AGACGTGCGG
CTTCCGTTTG
TCACGTCCGG
CACGCCGCGA
ACCGCAAGGA
ACCTTCCCGA
CTTAGGGGCG
GAGCAGGAAG
CGTCGCCGGG
GGGCCCACAA
GGGTAGCGGC
GAAGATCCGG
GTGACGCTGC
GAACGGACGT
GAAGAATGTG
CGAGACCCAG
GGTCGGCGCC
GCTGCGTTTC
CCGGAACCAC
GCCCAGAGCA
GCCGCGTCCC
TGCGCAAACC
CAGGGCTGCC
TTGGAAAAGG
CGCAACCCCA
ACCCCGTGGA
ATTATCACCT
CGAGTTTACT
CCCTATCAGT
GATAGAGAAC
GTATGAAGAG
TTTACTCCCT
ATCAGTGATA
GAGAACGTAT
GCAGACTTTA
CTCCCTATCA
GTGATAGAGA
ACGTATAAGG
AGTTTACTCC
CTATCAGTGA
TAGAGAACGT
ATGACCAGTT
TACTCCCTAT
CAGTGATAGA
GAACGTATCT
ACAGTTTACT
CCCTATCAGT
GATAGAGAAC
GTATATCCAG
TTTACTCCCT
ATCAGTGATA
GAGAACGTAT
AAGCTTTGCT
TATGTAAACC
AGGGCGCCTA
TAAAAGAGTG
CTGATTTTTT
GAGTAAACTT
CAATTCCACA
ACACTTTTGT
CTTATACCAA
CTTTCCGTAC
CACTTCCTAC
CCTCGTAAAG
AATTCGCCAC
CATGGTGAGC
AAGGGCGAGG
AGCTGTTCAC
CGGGGTGGTG
CCCATCCTGG
TCGAGCTGGA
CGGCGACGTA
AACGGCCACA
AGTTCAGCGT
GTCCGGCGAG
GGCGAGGGCG
ATGCCACCTA
CGGCAAGCTG
ACCCTGAAGT
TCATCTGCAC
CACCGGCAAG
CTGCCCGTGC
CCTGGCCCAC
CCTCGTGACC
ACCCTGACCT
ACGGCGTGCA
GTGCTTCAGC
CGCTACCCCG
ACCACATGAA
GCAGCACGAC
TTCTTCAAGT
CCGCCATGCC
CGAAGGCTAC
GTCCAGGAGC
GCACCATCTT
CTTCAAGGAC
GACGGCAACT
ACAAGACCCG
CGCCGAGGTG
AAGTTCGAGG
GCGACACCCT
GGTGAACCGC
ATCGAGCTGA
AGGGCATCGA
CTTCAAGGAG
GACGGCAACA
TCCTGGGGCA
CAAGCTGGAG
TACAACTACA
ACAGCCACAA
CGTCTATATC
ATGGCCGACA
AGCAGAAGAA
CGGCATCAAG
GTGAACTTCA
AGATCCGCCA
CAACATCGAG
GACGGCAGCG
TGCAGCTCGC
CGACCACTAC
CAGCAGAACA
CCCCCATCGG
CGACGGCCCC
GTGCTGCTGC
CCGACAACCA
CTACCTGAGC
ACCCAGTCCG
CCCTGAGCAA
AGACCCCAAC
GAGAAGCGCG
ATCACATGGT
CCTGCTGGAG
TTCGTGACCG
CCGCCGGGAT
CACTCTCGGC
ATGGACGAGC
TGTACAAGTA
GGGATCCAGA
CCACCTCCCC
TGCGAGCTAA
GCTGGACAGC
CAATGACGGG
TAAGAGAGTG
ACATTTTTCA
CTAACCTAAG
ACAGGAGGGC
CGTCAGAGCT
ACTGCCTAAT
CCAAAGACGG
GTAAAAGTGA
TAAAAATGTA
TCACTCCAAC
CTAAGACAGG
CGCAGCTTCC
GAGGGATTTG
AGATCCAGAC
ATGATAAGAT
ACATTGATGA
GTTTGGACAA
ACCAAAACTA
GAATGCAGTG
AAAAAAATGC
CTTATTTGTG
AAATTTGTGA
TGCTATTGCC
TTATTTGTAA
CCATTATAAG
CTGCAATAAA
CAAGTTAAGT
CGATAAACTG
GATCTCTGCT
GTCCCTGTAA
TAAACCCGAA
AATTTTGAAT
TTTTGTAATT
TGTTTTTGTA
GTTCTTTAGT
TTGTATGTCT
GTTGCTATTA
TGTCTACTAT
TCTTTCCCCT
GCACTGTACC
CCCCAATCCC
CCCTTTTCTT
TTAAAGGCGA
TACCGTCGAG
ATCCGTTCAC
TAATCGAATG
GATCTGTCTC
TGTCTCTCTC
TCCACCTTCT
TCTTCTATTC
CTTCGGGCCT
GTCGGGTCCC
CTCGGGGTTG
GGAGGTGGGT
CTGAAACGAT
AATGGTGAAT
ATCCCTGCCT
AACTCTATTC
ACTATAGAAA
GTACAGCAAA
AACTATTCTT
AAACCTACCA
AGCCTCCTAC
TATCATTATG
AATAATTTTA
TATACCACAG
CCAATTTGTT
ATGTTAAACC
AATTCCACAA
ACTTGCCCAT
TTATCTAATT
CCAATAATTC
TTGTTCATTC
TTTTCTTGCT
GGTTTTGCGA
TTCTTCAATT
AAGGAGTGTA
TTAAGCTTGT
GTAATTGTTA
ATTTCTCTGT
CCCACTCCAT
CCAGGTCGTG
TGATTCCAAA
TCTGTTCCAG
AGATTTATTA
CTCCAACTAG
CATTCCAAGG
CACAGCAGTG
GTGCAAATGA
GTTTTCCAGA
GCAACCCCAA
ATCCCCAGGA
GCTGTTGATC
CTTTAGGTAT
CTTTCCACAG
CCAGGATTCT
TGCCTGGAGC
TGCTTGATGC
CCCAGACTGT
GAGTTGCAAC
AGATGCTGTT
GCGCCTCAAT
AGCCCTCAGC
AAATTGTTCT
GCTGCTGCAC
TATACCAGAC
AATAATTGTC
TGGCCTGTAC
CGTCAGCGTC
ATTGACGCTG
CGCCCATAGT
GCTTCCTGCT
GCTCCCAAGA
ACCCAAGGAA
CAAAGCTCCT
ATTCCCACTG
CTCTTTTTTC
TCTCTGCACC
ACTCTTCTCT
TTGCCTTGGT
GGGTGCTACT
CCTAATGGTT
CAATTTTTAC
TACTTTATAT
TTATATAATT
CACTTCTCCA
ATTGTCCCTC
ATATCTCCTC
CTCCAGGTCT
GAAGATCAGC
GGCCGGCCGC
TTGCTGTGCG
GTGGTCTTAC
TTTTGTTTTG
CTCTTCCTCT
ATCTTGTCTA
AAGCTTCCTT
GGTGTCTTTT
ATCTCTATCC
TTTGATGCAC
ACAATAGAGG
GTTGCTACTG
TATTATATAA
TGATCTAAGT
TCTTCTGATC
CTGTCTGAAG
GGATGGTTGT
AGCTGTCCCA
GTATTTGTCT
ACAGCCTTCT
GATGTTTCTA
ACAGGCCAGG
ATTAACTGCG
AATCGTTCTA
GCTCCCTGCT
TGCCCATACT
ATATGTTTTA
ATTTATATTT
TTTCTTTCCC
CCTGGCCTTA
ACCGAATTTT
TTCCCATCGC
GATCTAATTC
TCCCCCGCTT
AATACTGACG
CTCTCGCACC
CATCTCTCTC
CTTCTAGCCT
CCGCTAGTCA
AAATTTTTGG
CGTACTCACC
AGTCGCCGCC
CCTCGCCTCT
TGCCGTGCGC
GCTTCAGCAA
GCCGAGTCCT
GCGTCGAGAG
AGCTCCTCTG
GTTTCCCTTT
CGCTTTCAAG
TCCCTGTTCG
GGCGCCACTG
CTAGAGATTT
TCCACACTGA
CTAAAAGGGT
CTGAGGGATC
TCTAGTTACC
AGAGTCACAC
AACAGACGGG
CACACACTAC
TTGAAGCACT
CAAGGCAAGC
TTTATTGAGG
CTTAAGCAGT
GGGTTCCCTA
GTTAGCCAGA
GAGCTCCCAG
GCTCAGATCT
GGTCTAACCA
GAGAGACCCA
GTACAGGCAA
AAAGCAGCTG
CTTATATGCA
GGATCTGAGG
GCTCGCCACT
CCCCAGTCCC
GCCCAGGCCA
CGCCTCCCTG
GAAAGTCCCC
AGCGGAAAGT
CCCTTGTAGC
AAGCTCGATA
TCAGCAGTTC
TTGAAGTACT
CCGGATGCAG
CTCTCGGGCC
ACGTGATGAA
ATGCTAGGCG
GCTGTCAAAC
CTCCACTCTA
ACACTTCTCT
CTCCGGGTCA
TCCATCCCAT
GCAGGCTCAC
AGGGTGTAAC
AAGCTGGTGT
TCTCTCCTTT
ATTGGCCTCT
TCTACCTTAT
CTGGCTCAAC
TGGTACTAGC
TTGTAGCACC
ATCCAAAGGT
CAGTGGATAT
CTGACCCCTG
GCCCTGGTGT
GTAGTTCTGC
TAATCAGGGA
AGTAGCCTTG
TGTGTGGTAG
ATCCACAGAT
CAAGGATATC
TTGTCTTCTT
TGGGAGTGAA
TTAGCCCTTC
CA