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pLV3-EF1a-CD40LG(human)-Puro

产品货号:P58523

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pLV3-EF1a-CD40LG(human)-Puro
别称: NM_000074.3;CD154; CD40L; gp39; hCD40L; HIGM1; IGM;IMD3; T-BAM; TNFSF5; TRAP
基因长: 786bp
质粒宿主: 哺乳细胞,慢病毒
质粒用途: 蛋白表达
片段类型: ORF
片段物种:
原核抗性: Amp
筛选标记: Puro
荧光标记:
启动子: EF1a
复制子: pUC
拷贝数:
感受态: Stbl3
温度: 37度
背景载体:
正向引物: PGK-R
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

ACGCGTGTAG

TCTTATGCAA

TACTCTTGTA

GTCTTGCAAC

ATGGTAACGA

TGAGTTAGCA

61

ACATGCCTTA

CAAGGAGAGA

AAAAGCACCG

TGCATGCCGA

TTGGTGGAAG

TAAGGTGGTA

121

CGATCGTGCC

TTATTAGGAA

GGCAACAGAC

GGGTCTGACA

TGGATTGGAC

GAACCACTGA

181

ATTGCCGCAT

TGCAGAGATA

TTGTATTTAA

GTGCCTAGCT

CGATACAATA

AACGGGTCTC

241

TCTGGTTAGA

CCAGATCTGA

GCCTGGGAGC

TCTCTGGCTA

ACTAGGGAAC

CCACTGCTTA

301

AGCCTCAATA

AAGCTTGCCT

TGAGTGCTTC

AAGTAGTGTG

TGCCCGTCTG

TTGTGTGACT

361

CTGGTAACTA

GAGATCCCTC

AGACCCTTTT

AGTCAGTGTG

GAAAATCTCT

AGCAGTGGCG

421

CCCGAACAGG

GACCTGAAAG

CGAAAGGGAA

ACCAGAGCTC

TCTCGACGCA

GGACTCGGCT

481

TGCTGAAGCG

CGCACGGCAA

GAGGCGAGGG

GCGGCGACTG

GTGAGTACGC

CAAAAATTTT

541

GACTAGCGGA

GGCTAGAAGG

AGAGAGATGG

GTGCGAGAGC

GTCAGTATTA

AGCGGGGGAG

601

AATTAGATCG

CGATGGGAAA

AAATTCGGTT

AAGGCCAGGG

GGAAAGAAAA

AATATAAATT

661

AAAACATATA

GTATGGGCAA

GCAGGGAGCT

AGAACGATTC

GCAGTTAATC

CTGGCCTGTT

721

AGAAACATCA

GAAGGCTGTA

GACAAATACT

GGGACAGCTA

CAACCATCCC

TTCAGACAGG

781

ATCAGAAGAA

CTTAGATCAT

TATATAATAC

AGTAGCAACC

CTCTATTGTG

TGCATCAAAG

841

GATAGAGATA

AAAGACACCA

AGGAAGCTTT

AGACAAGATA

GAGGAAGAGC

AAAACAAAAG

901

TAAGACCACC

GCACAGCAAG

CGGCCACTGA

TCTTCAGACC

TGGAGGAGGA

GATATGAGGG

961

ACAATTGGAG

AAGTGAATTA

TATAAATATA

AAGTAGTAAA

AATTGAACCA

TTAGGAGTAG

1021

CACCCACCAA

GGCAAAGAGA

AGAGTGGTGC

AGAGAGAAAA

AAGAGCAGTG

GGAATAGGAG

1081

CTTTGTTCCT

TGGGTTCTTG

GGAGCAGCAG

GAAGCACTAT

GGGCGCAGCC

TCAATGACGC

1141

TGACGGTACA

GGCCAGACAA

TTATTGTCTG

GTATAGTGCA

GCAGCAGAAC

AATTTGCTGA

1201

GGGCTATTGA

GGCGCAACAG

CATCTGTTGC

AACTCACAGT

CTGGGGCATC

AAGCAGCTCC

1261

AGGCAAGAAT

CCTGGCTGTG

GAAAGATACC

TAAAGGATCA

ACAGCTCCTG

GGGATTTGGG

1321

GTTGCTCTGG

AAAACTCATT

TGCACCACTG

CTGTGCCTTG

GAATGCTAGT

TGGAGTAATA

1381

AATCTCTGGA

ACAGATTGGA

ATCACACGAC

CTGGATGGAG

TGGGACAGAG

AAATTAACAA

1441

TTACACAAGC

TTAATACACT

CCTTAATTGA

AGAATCGCAA

AACCAGCAAG

AAAAGAATGA

1501

ACAAGAATTA

TTGGAATTAG

ATAAATGGGC

AAGTTTGTGG

AATTGGTTTA

ACATAACAAA

1561

TTGGCTGTGG

TATATAAAAT

TATTCATAAT

GATAGTAGGA

GGCTTGGTAG

GTTTAAGAAT

1621

AGTTTTTGCT

GTACTTTCTA

TAGTGAATAG

AGTTAGGCAG

GGATATTCAC

CATTATCGTT

1681

TCAGACCCAC

CTCCCAACCC

CGAGGGGACC

CGACAGGCCC

GAAGGAATAG

AAGAAGAAGG

1741

TGGAGAGAGA

GACAGAGACA

GATCCATTCG

ATTAGTGAAC

GGATCTCGAC

GGTTAACTTT

1801

TAAAAGAAAA

GGGGGGATTG

GGGGGTACAG

TGCAGGGGAA

AGAATAGTAG

ACATAATAGC

1861

AACAGACATA

CAAACTAAAG

AATTACAAAA

ACAAATTACA

AAAATTCAAA

ATTTTATCGA

1921

TACTAGTAAG

GATCTGCGAT

CGCTCCGGTG

CCCGTCAGTG

GGCAGAGCGC

ACATCGCCCA

1981

CAGTCCCCGA

GAAGTTGGGG

GGAGGGGTCG

GCAATTGAAC

GGGTGCCTAG

AGAAGGTGGC

2041

GCGGGGTAAA

CTGGGAAAGT

GATGTCGTGT

ACTGGCTCCG

CCTTTTTCCC

GAGGGTGGGG

2101

GAGAACCGTA

TATAAGTGCA

GTAGTCGCCG

TGAACGTTCT

TTTTCGCAAC

GGGTTTGCCG

2161

CCAGAACACA

GCTGAAGCTT

CGAGGGGCTC

GCATCTCTCC

TTCACGCGCC

CGCCGCCCTA

2221

CCTGAGGCCG

CCATCCACGC

CGGTTGAGTC

GCGTTCTGCC

GCCTCCCGCC

TGTGGTGCCT

2281

CCTGAACTGC

GTCCGCCGTC

TAGGTAAGTT

TAAAGCTCAG

GTCGAGACCG

GGCCTTTGTC

2341

CGGCGCTCCC

TTGGAGCCTA

CCTAGACTCA

GCCGGCTCTC

CACGCTTTGC

CTGACCCTGC

2401

TTGCTCAACT

CTACGTCTTT

GTTTCGTTTT

CTGTTCTGCG

CCGTTACAGA

TCCAAGCTGT

2461

GACCGGCGCC

TACTCTAGAG

CTAGCGAATT

CGCCACCATG

ATCGAAACAT

ACAACCAAAC

2521

TTCTCCCCGA

TCTGCGGCCA

CTGGACTGCC

CATCAGCATG

AAAATTTTTA

TGTATTTACT

2581

TACTGTTTTT

CTTATCACCC

AGATGATTGG

GTCAGCACTT

TTTGCTGTGT

ATCTTCATAG

2641

AAGGTTGGAC

AAGATAGAAG

ATGAAAGGAA

TCTTCATGAA

GATTTTGTAT

TCATGAAAAC

2701

GATACAGAGA

TGCAACACAG

GAGAAAGATC

CTTATCCTTA

CTGAACTGTG

AGGAGATTAA

2761

AAGCCAGTTT

GAAGGCTTTG

TGAAGGATAT

AATGTTAAAC

AAAGAGGAGA

CGAAGAAAGA

2821

AAACAGCTTT

GAAATGCAAA

AAGGTGATCA

GAATCCTCAA

ATTGCGGCAC

ATGTCATAAG

2881

TGAGGCCAGC

AGTAAAACAA

CATCTGTGTT

ACAGTGGGCT

GAAAAAGGAT

ACTACACCAT

2941

GAGCAACAAC

TTGGTAACCC

TGGAAAATGG

GAAACAGCTG

ACCGTTAAAA

GACAAGGACT

3001

CTATTATATC

TATGCCCAAG

TCACCTTCTG

TTCCAATCGG

GAAGCTTCGA

GTCAAGCTCC

3061

ATTTATAGCC

AGCCTCTGCC

TAAAGTCCCC

CGGTAGATTC

GAGAGAATCT

TACTCAGAGC

3121

TGCAAATACC

CACAGTTCCG

CCAAACCTTG

CGGGCAACAA

TCCATTCACT

TGGGAGGAGT

3181

ATTTGAATTG

CAACCAGGTG

CTTCGGTGTT

TGTCAATGTG

ACTGATCCAA

GCCAAGTGAG

3241

CCATGGCACT

GGCTTCACGT

CCTTTGGCTT

ACTCAAACTC

TGAGCGGCCG

CTGGGTAGGG

3301

GAGGCGCTTT

TCCCAAGGCA

GTCTGGAGCA

TGCGCTTTAG

CAGCCCCGCT

GGGCACTTGG

3361

CGCTACACAA

GTGGCCTCTG

GCCTCGCACA

CATTCCACAT

CCACCGGTAG

GCGCCAACCG

3421

GCTCCGTTCT

TTGGTGGCCC

CTTCGCGCCA

CCTTCTACTC

CTCCCCTAGT

CAGGAAGTTC

3481

CCCCCCGCCC

CGCAGCTCGC

GTCGTGCAGG

ACGTGACAAA

TGGAAGTAGC

ACGTCTCACT

3541

AGTCTCGTGC

AGATGGACAG

CACCGCTGAG

CAATGGAAGC

GGGTAGGCCT

TTGGGGCAGC

3601

GGCCAATAGC

AGCTTTGCTC

CTTCGCTTTC

TGGGCTCAGA

GGCTGGGAAG

GGGTGGGTCC

3661

GGGGGCGGGC

TCAGGGGCGG

GCTCAGGGGC

GGGGCGGGCG

CCCGAAGGTC

CTCCGGAGGC

3721

CCGGCATTCT

GCACGCTTCA

AAAGCGCACG

TCTGCCGCGC

TGTTCTCCTC

TTCCTCATCT

3781

CCGGGCCTTT

CGACCTGCAG

CCCAAGCTTA

CCATGACCGA

GTACAAGCCC

ACGGTGCGCC

3841

TCGCCACCCG

CGACGACGTC

CCCAGGGCCG

TACGCACCCT

CGCCGCCGCG

TTCGCCGACT

3901

ACCCCGCCAC

GCGCCACACC

GTCGATCCGG

ACCGCCACAT

CGAGCGGGTC

ACCGAGCTGC

3961

AAGAACTCTT

CCTCACGCGC

GTCGGGCTCG

ACATCGGCAA

GGTGTGGGTC

GCGGACGACG

4021

GCGCCGCGGT

GGCGGTCTGG

ACCACGCCGG

AGAGCGTCGA

AGCGGGGGCG

GTGTTCGCCG

4081

AGATCGGCCC

GCGCATGGCC

GAGTTGAGCG

GTTCCCGGCT

GGCCGCGCAG

CAACAGATGG

4141

AAGGCCTCCT

GGCGCCGCAC

CGGCCCAAGG

AGCCCGCGTG

GTTCCTGGCC

ACCGTCGGCG

4201

TCTCGCCCGA

CCACCAGGGC

AAGGGTCTGG

GCAGCGCCGT

CGTGCTCCCC

GGAGTGGAGG

4261

CGGCCGAGCG

CGCCGGGGTG

CCCGCCTTCC

TGGAGACCTC

CGCGCCCCGC

AACCTCCCCT

4321

TCTACGAGCG

GCTCGGCTTC

ACCGTCACCG

CCGACGTCGA

GGTGCCCGAA

GGACCGCGCA

4381

CCTGGTGCAT

GACCCGCAAG

CCCGGTGCCT

GAGTCGACAA

TCAACCTCTG

GATTACAAAA

4441

TTTGTGAAAG

ATTGACTGGT

ATTCTTAACT

ATGTTGCTCC

TTTTACGCTA

TGTGGATACG

4501

CTGCTTTAAT

GCCTTTGTAT

CATGCTATTG

CTTCCCGTAT

GGCTTTCATT

TTCTCCTCCT

4561

TGTATAAATC

CTGGTTGCTG

TCTCTTTATG

AGGAGTTGTG

GCCCGTTGTC

AGGCAACGTG

4621

GCGTGGTGTG

CACTGTGTTT

GCTGACGCAA

CCCCCACTGG

TTGGGGCATT

GCCACCACCT

4681

GTCAGCTCCT

TTCCGGGACT

TTCGCTTTCC

CCCTCCCTAT

TGCCACGGCG

GAACTCATCG

4741

CCGCCTGCCT

TGCCCGCTGC

TGGACAGGGG

CTCGGCTGTT

GGGCACTGAC

AATTCCGTGG

4801

TGTTGTCGGG

GAAATCATCG

TCCTTTCCTT

GGCTGCTCGC

CTGTGTTGCC

ACCTGGATTC

4861

TGCGCGGGAC

GTCCTTCTGC

TACGTCCCTT

CGGCCCTCAA

TCCAGCGGAC

CTTCCTTCCC

4921

GCGGCCTGCT

GCCGGCTCTG

CGGCCTCTTC

CGCGTCTTCG

CCTTCGCCCT

CAGACGAGTC

4981

GGATCTCCCT

TTGGGCCGCC

TCCCCGCCTG

GTACCTTTAA

GACCAATGAC

TTACAAGGCA

5041

GCTGTAGATC

TTAGCCACTT

TTTAAAAGAA

AAGGGGGGAC

TGGAAGGGCT

AATTCACTCC

5101

CAACGAAAAT

AAGATCTGCT

TTTTGCTTGT

ACTGGGTCTC

TCTGGTTAGA

CCAGATCTGA

5161

GCCTGGGAGC

TCTCTGGCTA

ACTAGGGAAC

CCACTGCTTA

AGCCTCAATA

AAGCTTGCCT

5221

TGAGTGCTTC

AAGTAGTGTG

TGCCCGTCTG

TTGTGTGACT

CTGGTAACTA

GAGATCCCTC

5281

AGACCCTTTT

AGTCAGTGTG

GAAAATCTCT

AGCAGTAGTA

GTTCATGTCA

TCTTATTATT

5341

CAGTATTTAT

AACTTGCAAA

GAAATGAATA

TCAGAGAGTG

AGAGGAACTT

GTTTATTGCA

5401

GCTTATAATG

GTTACAAATA

AAGCAATAGC

ATCACAAATT

TCACAAATAA

AGCATTTTTT

5461

TCACTGCATT

CTAGTTGTGG

TTTGTCCAAA

CTCATCAATG

TATCTTATCA

TGTCTGGCTC

5521

TAGCTATCCC

GCCCCTAACT

CCGCCCAGTT

CCGCCCATTC

TCCGCCCCAT

GGCTGACTAA

5581

TTTTTTTTAT

TTATGCAGAG

GCCGAGGCCG

CCTCGGCCTC

TGAGCTATTC

CAGAAGTAGT

5641

GAGGAGGCTT

TTTTGGAGGC

CTAGACTTTT

GCAGAGACGG

CCCAAATTCG

TAATCATGGT

5701

CATAGCTGTT

TCCTGTGTGA

AATTGTTATC

CGCTCACAAT

TCCACACAAC

ATACGAGCCG

5761

GAAGCATAAA

GTGTAAAGCC

TGGGGTGCCT

AATGAGTGAG

CTAACTCACA

TTAATTGCGT

5821

TGCGCTCACT

GCCCGCTTTC

CAGTCGGGAA

ACCTGTCGTG

CCAGCTGCAT

TAATGAATCG

5881

GCCAACGCGC

GGGGAGAGGC

GGTTTGCGTA

TTGGGCGCTC

TTCCGCTTCC

TCGCTCACTG

5941

ACTCGCTGCG

CTCGGTCGTT

CGGCTGCGGC

GAGCGGTATC

AGCTCACTCA

AAGGCGGTAA

6001

TACGGTTATC

CACAGAATCA

GGGGATAACG

CAGGAAAGAA

CATGTGAGCA

AAAGGCCAGC

6061

AAAAGGCCAG

GAACCGTAAA

AAGGCCGCGT

TGCTGGCGTT

TTTCCATAGG

CTCCGCCCCC

6121

CTGACGAGCA

TCACAAAAAT

CGACGCTCAA

GTCAGAGGTG

GCGAAACCCG

ACAGGACTAT

6181

AAAGATACCA

GGCGTTTCCC

CCTGGAAGCT

CCCTCGTGCG

CTCTCCTGTT

CCGACCCTGC

6241

CGCTTACCGG

ATACCTGTCC

GCCTTTCTCC

CTTCGGGAAG

CGTGGCGCTT

TCTCATAGCT

6301

CACGCTGTAG

GTATCTCAGT

TCGGTGTAGG

TCGTTCGCTC

CAAGCTGGGC

TGTGTGCACG

6361

AACCCCCCGT

TCAGCCCGAC

CGCTGCGCCT

TATCCGGTAA

CTATCGTCTT

GAGTCCAACC

6421

CGGTAAGACA

CGACTTATCG

CCACTGGCAG

CAGCCACTGG

TAACAGGATT

AGCAGAGCGA

6481

GGTATGTAGG

CGGTGCTACA

GAGTTCTTGA

AGTGGTGGCC

TAACTACGGC

TACACTAGAA

6541

GGACAGTATT

TGGTATCTGC

GCTCTGCTGA

AGCCAGTTAC

CTTCGGAAAA

AGAGTTGGTA

6601

GCTCTTGATC

CGGCAAACAA

ACCACCGCTG

GTAGCGGTGG

TTTTTTTGTT

TGCAAGCAGC

6661

AGATTACGCG

CAGAAAAAAA

GGATCTCAAG

AAGATCCTTT

GATCTTTTCT

ACGGGGTCTG

6721

ACGCTCAGTG

GAACGAAAAC

TCACGTTAAG

GGATTTTGGT

CATGAGATTA

TCAAAAAGGA

6781

TCTTCACCTA

GATCCTTTTA

AATTAAAAAT

GAAGTTTTAA

ATCAATCTAA

AGTATATATG

6841

AGTAAACTTG

GTCTGACAGT

TACCAATGCT

TAATCAGTGA

GGCACCTATC

TCAGCGATCT

6901

GTCTATTTCG

TTCATCCATA

GTTGCCTGAC

TCCCCGTCGT

GTAGATAACT

ACGATACGGG

6961

AGGGCTTACC

ATCTGGCCCC

AGTGCTGCAA

TGATACCGCG

AGACCCACGC

TCACCGGCTC

7021

CAGATTTATC

AGCAATAAAC

CAGCCAGCCG

GAAGGGCCGA

GCGCAGAAGT

GGTCCTGCAA

7081

CTTTATCCGC

CTCCATCCAG

TCTATTAATT

GTTGCCGGGA

AGCTAGAGTA

AGTAGTTCGC

7141

CAGTTAATAG

TTTGCGCAAC

GTTGTTGCCA

TTGCTACAGG

CATCGTGGTG

TCACGCTCGT

7201

CGTTTGGTAT

GGCTTCATTC

AGCTCCGGTT

CCCAACGATC

AAGGCGAGTT

ACATGATCCC

7261

CCATGTTGTG

CAAAAAAGCG

GTTAGCTCCT

TCGGTCCTCC

GATCGTTGTC

AGAAGTAAGT

7321

TGGCCGCAGT

GTTATCACTC

ATGGTTATGG

CAGCACTGCA

TAATTCTCTT

ACTGTCATGC

7381

CATCCGTAAG

ATGCTTTTCT

GTGACTGGTG

AGTACTCAAC

CAAGTCATTC

TGAGAATAGT

7441

GTATGCGGCG

ACCGAGTTGC

TCTTGCCCGG

CGTCAATACG

GGATAATACC

GCGCCACATA

7501

GCAGAACTTT

AAAAGTGCTC

ATCATTGGAA

AACGTTCTTC

GGGGCGAAAA

CTCTCAAGGA

7561

TCTTACCGCT

GTTGAGATCC

AGTTCGATGT

AACCCACTCG

TGCACCCAAC

TGATCTTCAG

7621

CATCTTTTAC

TTTCACCAGC

GTTTCTGGGT

GAGCAAAAAC

AGGAAGGCAA

AATGCCGCAA

7681

AAAAGGGAAT

AAGGGCGACA

CGGAAATGTT

GAATACTCAT

ACTCTTCCTT

TTTCAATATT

7741

ATTGAAGCAT

TTATCAGGGT

TATTGTCTCA

TGAGCGGATA

CATATTTGAA

TGTATTTAGA

7801

AAAATAAACA

AATAGGGGTT

CCGCGCACAT

TTCCCCGAAA

AGTGCCACCT

GACGTCTAAG

7861

AAACCATTAT

TATCATGACA

TTAACCTATA

AAAATAGGCG

TATCACGAGG

CCCTTTCGTC

7921

TCGCGCGTTT

CGGTGATGAC

GGTGAAAACC

TCTGACACAT

GCAGCTCCCG

GAGACGGTCA

7981

CAGCTTGTCT

GTAAGCGGAT

GCCGGGAGCA

GACAAGCCCG

TCAGGGCGCG

TCAGCGGGTG

8041

TTGGCGGGTG

TCGGGGCTGG

CTTAACTATG

CGGCATCAGA

GCAGATTGTA

CTGAGAGTGC

8101

ACCATATGCG

GTGTGAAATA

CCGCACAGAT

GCGTAAGGAG

AAAATACCGC

ATCAGGCGCC

8161

ATTCGCCATT

CAGGCTGCGC

AACTGTTGGG

AAGGGCGATC

GGTGCGGGCC

TCTTCGCTAT

8221

TACGCCAGCT

GGCGAAAGGG

GGATGTGCTG

CAAGGCGATT

AAGTTGGGTA

ACGCCAGGGT

8281

TTTCCCAGTC

ACGACGTTGT

AAAACGACGG

CCAGTGCCAA

GCTG

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