名称: | pLV3-CMV-Plxna4(mouse)-3×FLAG-CopGFP-Puro |
---|---|
别称: | NM_175750.3;9330117B14; mKIAA1550; Plxa4; |
基因长: | 5679bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 绿色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | pLV3-CMV-MCS-3×FLAG-CopGFP-Puro |
正向引物: | CMV-F:CGCAAATGGGCGGTAGGCGTG |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
acgcgtgtag
tcttatgcaa
tactcttgta
gtcttgcaac
atggtaacga
tgagttagca
acatgcctta
caaggagaga
aaaagcaccg
tgcatgccga
ttggtggaag
taaggtggta
cgatcgtgcc
ttattaggaa
ggcaacagac
gggtctgaca
tggattggac
gaaccactga
attgccgcat
tgcagagata
ttgtatttaa
gtgcctagct
cgatacaata
aacgggtctc
tctggttaga
ccagatctga
gcctgggagc
tctctggcta
actagggaac
ccactgctta
agcctcaata
aagcttgcct
tgagtgcttc
aagtagtgtg
tgcccgtctg
ttgtgtgact
ctggtaacta
gagatccctc
agaccctttt
agtcagtgtg
gaaaatctct
agcagtggcg
cccgaacagg
gacctgaaag
cgaaagggaa
accagagctc
tctcgacgca
ggactcggct
tgctgaagcg
cgcacggcaa
gaggcgaggg
gcggcgactg
gtgagtacgc
caaaaatttt
gactagcgga
ggctagaagg
agagagatgg
gtgcgagagc
gtcagtatta
agcgggggag
aattagatcg
cgatgggaaa
aaattcggtt
aaggccaggg
ggaaagaaaa
aatataaatt
aaaacatata
gtatgggcaa
gcagggagct
agaacgattc
gcagttaatc
ctggcctgtt
agaaacatca
gaaggctgta
gacaaatact
gggacagcta
caaccatccc
ttcagacagg
atcagaagaa
cttagatcat
tatataatac
agtagcaacc
ctctattgtg
tgcatcaaag
gatagagata
aaagacacca
aggaagcttt
agacaagata
gaggaagagc
aaaacaaaag
taagaccacc
gcacagcaag
cggccactga
tcttcagacc
tggaggagga
gatatgaggg
acaattggag
aagtgaatta
tataaatata
aagtagtaaa
aattgaacca
ttaggagtag
cacccaccaa
ggcaaagaga
agagtggtgc
agagagaaaa
aagagcagtg
ggaataggag
ctttgttcct
tgggttcttg
ggagcagcag
gaagcactat
gggcgcagcc
tcaatgacgc
tgacggtaca
ggccagacaa
ttattgtctg
gtatagtgca
gcagcagaac
aatttgctga
gggctattga
ggcgcaacag
catctgttgc
aactcacagt
ctggggcatc
aagcagctcc
aggcaagaat
cctggctgtg
gaaagatacc
taaaggatca
acagctcctg
gggatttggg
gttgctctgg
aaaactcatt
tgcaccactg
ctgtgccttg
gaatgctagt
tggagtaata
aatctctgga
acagattgga
atcacacgac
ctggatggag
tgggacagag
aaattaacaa
ttacacaagc
ttaatacact
ccttaattga
agaatcgcaa
aaccagcaag
aaaagaatga
acaagaatta
ttggaattag
ataaatgggc
aagtttgtgg
aattggttta
acataacaaa
ttggctgtgg
tatataaaat
tattcataat
gatagtagga
ggcttggtag
gtttaagaat
agtttttgct
gtactttcta
tagtgaatag
agttaggcag
ggatattcac
cattatcgtt
tcagacccac
ctcccaaccc
cgaggggacc
cgacaggccc
gaaggaatag
aagaagaagg
tggagagaga
gacagagaca
gatccattcg
attagtgaac
ggatctcgac
ggtATCGGTt
aacttttaaa
agaaaagggg
ggattggggg
gtacagtgca
ggggaaagaa
tagtagacat
aatagcaaca
gacatacaaa
ctaaagaatt
acaaaaacaa
attacaaaaa
ttcaaaattt
tatcgatact
agtattatgc
ccagtacatg
accttatggg
actttcctac
ttggcagtac
atctacgtat
tagtcatcgc
tattaccatg
gtgatgcggt
tttggcagta
catcaatggg
cgtggatagc
ggtttgactc
acggggattt
ccaagtctcc
accccattga
cgtcaatggg
agtttgtttt
ggcaccaaaa
tcaacgggac
tttccaaaat
gtcgtaacaa
ctccgcccca
ttgacgcaaa
tgggcggtag
gcgtgtacgg
tgggaggtTt
atataagcag
agctcgttta
gtgaaccgtc
agatcgcctg
gagacgccat
ccacgctgtt
ttgacctcca
tagaagattc
tagagctagc
CTGATACGAA
CTCGgaattc
GCCACCatga
aagccatgcc
ctggaactgg
acttgcctgc
tctcccacct
gctggtggta
gggatgggct
cctccactct
gcttccacga
cagccacccc
agctgtctca
gaagccttcc
tttgtgacat
tccgagggga
gcctgctgag
ggcttcaatc
accttgtggt
ggatgagagg
acagggcaca
tttatttggg
ggctgtcaac
agaatctaca
aactctctag
tgacctcaaa
gtcttggtga
ctcatcagac
agggccagat
gaggacaacc
ccaagtgtta
cccacctcgg
attgttcaga
cctgcaatga
gcctctggcc
agcaccaata
acgtcaacaa
gatgctgctt
atagactaca
aagaaaacag
gctgatcgcg
tgtgggagcc
tgtaccaggg
tatctgcaag
ctcctgaggc
tagaggacct
cttcaagttg
ggagagccct
ttcacaagaa
ggaacattat
ctctcggggg
ttaacgagag
tggctcagtc
tttggggtga
ttgtttccta
cagcaacttt
gatgataaac
tctttattgc
cacagcagtg
gatggcaaac
ctgaatattt
tcctaccatc
tccagcagga
agctgactaa
gaactctgag
gccgatggca
tgtttgctta
cgttttccat
gatgaatttg
tggcctctat
gattaagatc
ccttcagaca
ctttcacggt
tatccccgac
tttgacatct
actacgtcta
tggcttcagc
agtggcaatt
ttgtctactt
tttgaccctt
cagccagaga
tggtgtctcc
cccaggctcc
acgacaaagg
agcaggtgta
cacctcaaag
cttgtgaggc
tttgcaaaga
ggacacagcc
tttaactcct
acgtagaggt
gcccattggc
tgtgagcgca
atggggtgga
gtatcgcttg
ttacaggctg
cctacttgtc
taaagctggt
gcagtgcttg
gccggaccct
aggagtccgc
ccagatgacg
acctcctctt
caccgtcttc
tccaagggcc
agaagcggaa
gatgaaatct
ctggatgagt
ctgccctgtg
catcttcatc
ttgaagcaga
tcaatgatcg
cattaaggac
cgcctgcagt
cctgttatag
gggtgaggga
acactagacc
tggcctggct
caaggtgaag
gacatccctt
gcagcagtgc
gctccttact
attgatgaca
acttctgtgg
cctggatatg
aatgctcctc
tgggagtttc
cgaaatggtg
cgtggcattc
cagtcttcac
agaagacagg
gaccgcatga
cttctgtcat
cgcctatgtc
tacaagaacc
actcactggc
cttcgtgggc
accaaaagtg
gcaagctgaa
gaagatccgg
gttgatgggc
ccaagggaaa
tgccctccaa
tatgagactg
tgcaggtggt
ggactcagga
ccagtcctcc
gggacatggc
cttttccaag
gaccacgagc
aactctacat
catgtcagaa
aggcagctta
ccagagttcc
tgtggagtcc
tgtgggcagt
accggagctg
tggcgagtgt
cttggctcag
gtgaccccca
ttgtggctgg
tgtgtgctcc
acaacacgtg
cacccggaag
gaacggtgtg
aacgctccag
agaaccccga
aggtttgcct
cagagatgaa
gcagtgtgtc
cggctgacgg
tccatcccaa
caacatctct
gtctctcagt
acaacgtgct
gctggtccta
gagacataca
atgtcccgga
gctgtcagca
ggtgtcaact
gtacctttga
agatctgtca
gagatggatg
ggctggtaat
aggcaatcag
atccagtgct
actcccctgc
agccaaggaa
gtgccccgga
tcatcacaga
aaatggggac
catcatgttg
tccagctgca
actcaagtca
aaggagactg
ggatgacctt
cgccagcacc
agctttgtct
tctacaactg
cagtgtccac
aattcatgcc
tgtcctgtgt
ggagagcccg
taccgttgcc
actggtgtaa
ataccggcat
gtttgtactc
acgaccccaa
cacatgctcc
ttccaggaag
gccgagtgaa
gctacctgag
gactgccccc
agctgctgcg
agtggacaag
atcctcgttc
cagtagaggt
gatcaagccc
atcactctga
aggctaagaa
cctcccacag
cctcagtcgg
gacagagagg
ctacgagtgc
atcttgaaca
tccagggcat
cgagcagagg
gtacctgctc
tgcgcttcaa
cagctccagt
gtgcagtgtc
agaacacctc
ctattcctat
gaaggaatgg
agatcaacaa
cctgccagtg
gagttgacag
tcgtgtggaa
tggacacttc
aacattgaca
acccagctca
gaataaagtt
tacctctaca
agtgtggagc
catgcgcgag
agctgtgggc
tgtgcctcaa
ggccgacccg
gatttcgagt
gtggctggtg
tcagagccca
ggccagtgta
ccctgcgcca
gcactgccct
gcccatgaga
gccggtggtt
ggaactgtcg
ggggccaaca
gcaagtgcac
caaccctcgt
atcacagaga
tcatcccagt
gacgggccct
cgagaagggg
gcaccaaggt
caccatccga
ggggagaact
tgggcctgga
attccgtgac
attgcatcac
acgtgaaagt
ggctggtgtg
gaatgcagcc
ccttggtgga
tggctacatc
ccagctgaac
agatcgtgtg
tgagatgggt
gaggccaagc
ctagccagca
tgcaggcttt
gtggagatct
gtgtggctgt
gtgtcggcct
gagttcatgg
cccgctcttc
gcagctctat
tacttcatga
ctctgaccct
cgcagatctg
aagcccaacc
gaggacccat
gtctgggggg
acacaggtta
ccatcacagg
taccaacttg
aatgcaggca
gcaatgttgt
ggtgatgttc
gggagccagc
cctgcctctt
ccacaggcgc
tctccatcct
acattatctg
taacactaca
tcctcagagg
aggtgctgga
catgaaggtg
actgtgcagg
tggacagggc
caggatccgc
caggacctgg
tctttcagta
cgtggaggac
cccaccattg
tgcggattga
accagaatgg
agcattgtca
gtgggaacac
acctattgct
gtctggggaa
ctcacctgga
cctcatacag
aacccacaga
tccgtgccaa
gcatggagga
aaagaacaca
tcaacatctg
tgaggtccta
aatgctacag
agatgacctg
ccaggctcca
gcccttgccc
tgggtcccga
ccaccagtca
gatctcactg
agaggcctga
agaatttggt
ttcatcctgg
acaatgtcca
gtctctgcta
attctcaaca
agaccaactt
cacctattat
cctaaccccg
tgtttgaggc
tttcagtccc
tcgggaatcc
tggagctcaa
gccgggcacc
cccattatcc
taaagggcaa
gaacttgatc
ccgcctgtgg
ccggaggcaa
tgtgaagctg
aactacaccg
tgctggttgg
ggagaaacca
tgcaccgtga
cagtatcaga
tgtgcagctg
ctctgcgagt
ctcccaacct
catcggcagg
cacaaagtga
tggcacgggt
gggtggcatg
gagtattccc
ctgggatggt
atacatcgcc
ccagacagcc
cgctcagcct
gcccgccatt
gtcagcatcg
cggtggctgg
tggcctcctc
atcatcttca
tcgtggccgt
actcatcgcc
tacaaacgca
aatcccgaga
aagtgacctc
acgctgaaga
ggttgcagat
gcagatggac
aaccttgagt
cccgtgtggc
gctggagtgc
aaggaagctt
ttgccgagct
gcagacagat
atccatgagc
ttaccagtga
cctggatgga
gctgggattc
cctttctgga
ctacagaacc
tataccatgc
gggtgctgtt
cccagggatt
gaagaccacc
ctgtgctacg
agaccttgag
gtcccaggct
accggcagga
gcgtgtggag
aaaggactca
agctcttcgc
ccagctcatc
aacaacaagg
tgttcctgct
ctctttcatc
cgcactctgg
agtctcagcg
cagtttttcc
atgcgcgacc
gaggtaacgt
ggcctccctc
atcatgacgg
tacttcagag
caagctagag
tatgccactg
acgtgctgaa
gcagctactg
gctgacctca
ttgataagaa
cctggagagc
aagaaccacc
ccaagctgct
gctcaggagg
acggagtcgg
tggccgagaa
gatgttgact
aattggttca
ctttcctcct
ctacaagttc
ctcaaggagt
gtgctgggga
gccactcttc
tccctgttct
gtgccatcaa
gcagcagatg
gagaagggcc
ccattgatgc
catcaccgga
gaggcccgct
actcgctaag
tgaggacaaa
ctcatccgac
agcagattga
atataaaacc
ttggtcctga
gctgtgtcag
tccagataat
gttaacagcc
cagaggtccc
agtgaagatc
ctcaactgtg
acaccatcac
tcaggtcaaa
gagaagatcc
tagatgccat
cttcaagaat
gtcccgtgct
cccataggcc
caaagctgca
gacatggatc
tagagtggcg
acaaggaagt
ggggcgagga
tgatcctgca
ggacgaagat
atcaccacca
agatcgagaa
tgattggaag
agactcaaca
cagtagccca
ctaccaggta
ccagatggtt
ctgtggtggc
tttagtgtcc
aagcaagtga
cagcctacaa
tgcagtgaac
aactccactg
tctccaggac
ttcagcaagt
aaatatgaga
acatgatccg
gtacacaggc
agccctgaca
gcctccgttc
ccggacaccc
atgatcaccc
ctgacctgga
aagcggggtc
aagctttggc
atctggtgaa
gaaccatgaa
catggagacc
agaaggaggg
ggaccggggg
agcaagatgg
tgtctgagat
ctatctgaca
cgactattgg
ccacaaaggg
cacactgcag
aagtttgtgg
atgacctatt
cgagaccatc
ttcagtacgg
cccaccgtgg
ctccgctctg
ccgctagcca
tcaagtacat
gtttgacttc
ctcgatgagc
aggctgataa
gcacggcatc
catgacccgc
acgtccgcca
cacctggaag
agcaactgcc
tgcccctacg
gttttgggtg
aatatgatca
agaaccctca
gtttgtgttt
gacatccata
agaacagcat
cacggatgcc
tgtctctctg
tggtggccca
gaccttcatg
gactcctgct
ccacttcgga
gcaccgactg
ggcaaggatt
ccccctccaa
caagctgctc
tatgccaagg
acatccccag
ctacaagaac
tgggtagaga
ggtattattc
agacattggg
aagatgccgg
caataagtga
ccaggacatg
aatgcatacc
tggccgagca
gtcgcggatg
cacatgaatg
agttcaatac
gatgagtgcg
ctctcagaga
tcttctccta
tgtgggcaaa
tacagtgagg
agatccttgg
acccctggac
catgatgacc
agtgtggaaa
gcagaaactg
gcttacaaac
tagaacaagt
cataactctc
atgagcttag
acagcggatc
cGACTACAAA
GACCATGACG
GTGATTATAA
AGATCATGAC
ATCGACTACA
AGGATGACGA
TGACAAGtag
cggccgcGaa
ggatctgcga
tcgctccggt
gcccgtcagt
gggcagagcg
cacatcgccc
acagtccccg
agaagttggg
gggaggggtc
ggcaattgaa
cgggtgccta
gagaaggtgg
cgcggggtaa
actgggaaag
tgatgtcgtg
tactggctcc
gcctttttcc
cgagggtggg
ggagaaccgt
atataagtgc
agtagtcgcc
gtgaacgttc
tttttcgcaa
cgggtttgcc
gccagaacac
agctgaagct
tcgaggggct
cgcatctctc
cttcacgcgc
ccgccgccct
acctgaggcc
gccatccacg
ccggttgagt
cgcgttctgc
cgcctcccgc
ctgtggtgcc
tcctgaactg
cgtccgccgt
ctaggtaagt
ttaaagctca
ggtcgagacc
gggcctttgt
ccggcgctcc
cttggagcct
acctagactc
agccggctct
ccacgctttg
cctgaccctg
cttgctcaac
tctacgtctt
tgtttcgttt
tctgttctgc
gccgttacag
atccaagctg
tgaccggcgc
ctacgctaga
cgccaccatg
gagagcgacg
agagcggcct
gcccgccatg
gagatcgagt
gccgcatcac
cggcaccctg
aacggcgtgg
agttcgagct
ggtgggcggc
ggagagggca
cccccaagca
gggccgcatg
accaacaaga
tgaagagcac
caaaggcgcc
ctgaccttca
gcccctacct
gctgagccac
gtgatgggct
acggcttcta
ccacttcggc
acctacccca
gcggctacga
gaaccccttc
ctgcacgcca
tcaacaacgg
cggctacacc
aacacccgca
tcgagaagta
cgaggacggc
ggcgtgctgc
acgtgagctt
cagctaccgc
tacgaggccg
gccgcgtgat
cggcgacttc
aaggtggtgg
gcaccggctt
ccccgaggac
agcgtgatct
tcaccgacaa
gatcatccgc
agcaacgcca
ccgtggagca
cctgcacccc
atgggcgata
acgtgctggt
gggcagcttc
gcccgcacct
tcagcctgcg
cgacggcggc
tactacagct
tcgtggtgga
cagccacatg
cacttcaaga
gcgccatcca
ccccagcatc
ctgcagaacg
ggggccccat
gttcgccttc
cgccgcgtgg
aggagctgca
cagcaacacc
gagctgggca
tcgtggagta
ccagcacgcc
ttcaagaccc
ccatcgcctt
cgccagatcc
cgcgctcagt
cgtccaattc
tgccgtggac
ggcaccgccg
gacccggctc
caccggatct
cgcgagggca
gaggaagtct
tctaacatgc
ggtgacgtgg
aggagaatcc
cggccctatg
accgagtaca
agcccacggt
gcgcctcgcc
acccgcgacg
acgtccccag
ggccgtacgc
accctcgccg
ccgcgttcgc
cgactacccc
gccacgcgcc
acaccgtcga
tccggaccgc
cacatcgagc
gggtcaccga
gctgcaagaa
ctcttcctca
cgcgcgtcgg
gctcgacatc
ggcaaggtgt
gggtcgcgga
cgacggcgcc
gcggtggcgg
tctggaccac
gccggagagc
gtcgaagcgg
gggcggtgtt
cgccgagatc
ggcccgcgca
tggccgagtt
gagcggttcc
cggctggccg
cgcagcaaca
gatggaaggc
ctcctggcgc
cgcaccggcc
caaggagccc
gcgtggttcc
tggccaccgt
cggcgtctcg
cccgaccacc
agggcaaggg
tctgggcagc
gccgtcgtgc
tccccggagt
ggaggcggcc
gagcgcgccg
gggtgcccgc
cttcctggag
acctccgcgc
cccgcaacct
ccccttctac
gagcggctcg
gcttcaccgt
caccgccgac
gtcgaggtgc
ccgaaggacc
gcgcacctgg
tgcatgaccc
gcaagcccgg
tgcctgaaat
caacctctgg
attacaaaat
ttgtgaaaga
ttgactggta
ttcttaacta
tgttgctcct
tttacgctat
gtggatacgc
tgctttaatg
cctttgtatc
atgctattgc
ttcccgtatg
gctttcattt
tctcctcctt
gtataaatcc
tggttgctgt
ctctttatga
ggagttgtgg
cccgttgtca
ggcaacgtgg
cgtggtgtgc
actgtgtttg
ctgacgcaac
ccccactggt
tggggcattg
ccaccacctg
tcagctcctt
tccgggactt
tcgctttccc
cctccctatt
gccacggcgg
aactcatcgc
cgcctgcctt
gcccgctgct
ggacaggggc
tcggctgttg
ggcactgaca
attccgtggt
gttgtcgggg
aaATCATCGT
CCTTTCCTtg
gctgctcgcc
tgtgttgcca
cctggattct
gcgcgggacg
tccttctgct
acgtcccttc
ggccctcaat
ccagcggacc
ttccttcccg
cggcctgctg
ccggctctgc
ggcctcttcc
gcgtctTcgc
cttcgccctc
agacgagtcg
gatctccctt
tggGccgcct
ccccgcctgg
tacctttaag
accaatgact
tacaaggcag
ctgtagatct
tagccacttt
ttaaaagaaa
aggggggact
ggaagggcta
attcactccc
aacgaaGata
agatctgctt
tttgcttgta
ctgggtctct
ctggttagac
cagatctgag
cctgggagct
ctctggctaa
ctagggaacc
cactgcttaa
gcctcaataa
agcttgcctt
gagtgcttca
agtagtgtgt
gcccgtctgt
tgtgtgactc
tggtaactag
agatccctca
gaccctttta
gtcagtgtgg
aaaatctcta
gcagtagtag
ttcatgtcat
cttattattc
agtatttata
acttgcaaag
aaatgaatat
cagagagtga
gaggaacttg
tttattgcag
cttataatgg
ttacaaataa
agcaatagca
tcacaaattt
cacaaataaa
gcattttttt
cactgcattc
tagttgtggt
ttgtccaaac
tcatcaatgt
atcttatcat
gtctggctct
agctaTCCCG
CCCCTAACTC
CGCCCAtccc
gcccctaact
ccgcccagtt
ccgcccattc
tccgccccat
ggctgactaa
ttttttttat
ttatgcagag
gccgaggccg
cctcggcctc
tgagctattc
cagaagtagt
gaggaggctt
ttttggaggc
ctagactttt
gcagagacca
aattcgtaat
catgtcatag
ctgtttcctg
tgtgaaattg
ttatccgctc
acaattccac
acaacatacg
agccggaagc
ataaagtgta
aagcctgggg
tgcctaatga
gtgagctaac
tcacattaat
tgcgttgcgc
tcactgcccg
ctttccagtc
gggaaacctg
tcgtgccagc
tgcattaatg
aatcggccaa
cgcgcgggga
gaggcggttt
gcgtattggg
cgctcttccg
cttcctcgct
cactgactcg
ctgcgctcgg
tcgttcggct
gcggcgagcg
gtatcagctc
actcaaaggc
ggtaatacgg
ttatccacag
aatcagggga
taacgcagga
aagaacatgt
gagcaaaagg
ccagcaaaag
gccaggaacc
gtaaaaaggc
cgcgttgctg
gcgtttttcc
ataggctccg
cccccctgac
gagcatcaca
aaaatcgacg
ctcaagtcag
aggtggcgaa
acccgacagg
actataaaga
taccaggcgt
ttccccctgg
aagctccctc
gtgcgctctc
ctgttccgac
cctgccgctt
accggatacc
tgtccgcctt
tctcccttcg
ggaagcgtgg
cgctttctca
tagctcacgc
tgtaggtatc
tcagttcggt
gtaggtcgtt
cgctccaagc
tgggctgtgt
gcacgaaccc
cccgttcagc
ccgaccgctg
cgccttatcc
ggtaactatc
gtcttgagtc
caacccggta
agacacgact
tatcgccact
ggcagcagcc
actggtaaca
ggattagcag
agcgaggtat
gtaggcggtg
ctacagagtt
cttgaagtgg
tggcctaact
acggctacac
tagaaggaca
gtatttggta
tctgcgctct
gctgaagcca
gttaccttcg
gaaaaagagt
tggtagctct
tgatccggca
aacaaaccac
cgctggtagc
ggtggttttt
ttgtttgcaa
gcagcagatt
acgcgcagaa
aaaaaggatc
tcaagaagat
cctttgatct
tttctacggg
gtctgacgct
cagtggaacg
aaaactcacg
ttaagggatt
ttggtcatga
gattatcaaa
aaggatcttc
acctagatcc
ttttaaatta
aaaatgaagt
tttaaatcaa
tctaaagtat
atatgagtaa
acttggtctg
acagttacca
atgcttaatc
agtgaggcac
ctatctcagc
gatctgtcta
tttcgttcat
ccatagttgc
ctgactcccc
gtcgtgtaga
taactacgat
acgggagggc
ttaccatctg
gccccagtgc
tgcaatgata
ccgcgagacc
cacgctcacc
ggctccagat
ttatcagcaa
taaaccagcc
agccggaagg
gccgagcgca
gaagtggtcc
tgcaacttta
tccgcctcca
tccagtctat
taattgttgc
cgggaagcta
gagtaagtag
ttcgccagtt
aatagtttgc
gcaacgttgt
tgccattgct
acaggcatcg
tggtgtcacg
ctcgtcgttt
ggtatggctt
cattcagctc
cggttcccaa
cgatcaaggc
gagttacatg
atcccccatg
ttgtgcaaaa
aagcggttag
ctccttcggt
cctccgatcg
ttgtcagaag
taagttggcc
gcagtgttat
cactcatggt
tatggcagca
ctgcataatt
ctcttactgt
catgccatcc
gtaagatgct
tttctgtgac
tggtgagtac
tcaaccaagt
cattctgaga
atagtgtatg
cggcgaccga
gttgctcttg
cccggcgtca
atacgggata
ataccgcgcc
acatagcaga
actttaaaag
tgctcatcat
tggaaaacgt
tcttcggggc
gaaaactctc
aaggatctta
ccgctgttga
gatccagttc
gatgtaaccc
actcgtgcac
ccaactgatc
ttcagcatct
tttactttca
ccagcgtttc
tgggtgagca
aaaacaggaa
ggcaaaatgc
cgcaaaaaag
ggaataaggg
cgacacggaa
atgttgaata
ctcatactct
tcctttttca
atattattga
agcatttatc
agggttattg
tctcatgagc
ggatacatat
ttgaatgtat
ttagaaaaat
aaacaaatag
gggttccgcg
cacatttccc
cgaaaagtgc
cacctgacgt
ctaagaaacc
attattatca
tgacattaac
ctataaaaat
aggcgtatca
cgaggccctt
tcgtctcgcg
cgtttcggtg
atgacggtga
aaacctctga
cacatgcagc
tcccggagac
ggtcacagct
tgtctgtaag
cggatgccgg
gagcagacaa
gcccgtcagg
gcgcgtcagc
gggtgttggc
gggtgtcggg
gctggcttaa
ctatgcggca
tcagagcaga
ttgtactgag
agtgcaccat
atgcggtgtg
aaataccgca
cagatgcgta
aggagaaaat
accgcatcag
gcgccattcg
ccattcaggc
tgcgcaactg
ttgggaaggg
cgatcggtgc
gggcctcttc
gctattacgc
cagctggcga
aagggggatg
tgctgcaagg
cgattaagtt
gggtaacgcc
agggttttcc
cagtcacgac
gttgtaaaac
gacggccagt
gccaagctg