名称: | pCasKP-Ery |
---|---|
别称: | |
基因长: | |
质粒宿主: | 大肠杆菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR,Red |
片段物种: | |
原核抗性: | Ery |
筛选标记: | |
荧光标记: | |
启动子: | araBAD |
复制子: | pSC101 |
拷贝数: | 低 |
感受态: | DH5a |
温度: | 30度 |
背景载体: | |
正向引物: | G27066-F1 |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
AAGAAACCAA
TTGTCCATAT
TGCATCAGAC
ATTGCCGTCA
CTGCGTCTTT
TACTGGCTCT
TCTCGCTAAC
CAAACCGGTA
ACCCCGCTTA
TTAAAAGCAT
TCTGTAACAA
AGCGGGACCA
AAGCCATGAC
AAAAACGCGT
AACAAAAGTG
TCTATAATCA
CGGCAGAAAA
GTCCACATTG
ATTATTTGCA
CGGCGTCACA
CTTTGCTATG
CCATAGCATT
TTTATCCATA
AGATTAGCGG
ATCCTACCTG
ACGCTTTTTA
TCGCAACTCT
CTACTGTTTC
TCCATACCCG
TTTTTTTGGG
CTAGCGAAAA
GATGTTTCGT
GAAGCCGTCG
ACGCTTATAA
AAAATGGATA
TTAATACTGA
AACTGAGATC
AAGCAAAAGC
ATTCACTAAC
CCCCTTTCCT
GTTTTCCTAA
TCAGCCCGGC
ATTTCGCGGG
CGATATTTTC
ACAGCTATTT
CAGGAGTTCA
GCCATGAACG
CTTATTACAT
TCAGGATCGT
CTTGAGGCTC
AGAGCTGGGC
GCGTCACTAC
CAGCAGCTCG
CCCGTGAAGA
GAAAGAGGCA
GAACTGGCAG
ACGACATGGA
AAAAGGCCTG
CCCCAGCACC
TGTTTGAATC
GCTATGCATC
GATCATTTGC
AACGCCACGG
GGCCAGCAAA
AAATCCATTA
CCCGTGCGTT
TGATGACGAT
GTTGAGTTTC
AGGAGCGCAT
GGCAGAACAC
ATCCGGTACA
TGGTTGAAAC
CATTGCTCAC
CACCAGGTTG
ATATTGATTC
AGAGGTATAA
AACGAATGAG
TACTGCACTC
GCAACGCTGG
CTGGGAAGCT
GGCTGAACGT
GTCGGCATGG
ATTCTGTCGA
CCCACAGGAA
CTGATCACCA
CTCTTCGCCA
GACGGCATTT
AAAGGTGATG
CCAGCGATGC
GCAGTTCATC
GCATTACTGA
TCGTTGCCAA
CCAGTACGGC
CTTAATCCGT
GGACGAAAGA
AATTTACGCC
TTTCCTGATA
AGCAGAATGG
CATCGTTCCG
GTGGTGGGCG
TTGATGGCTG
GTCCCGCATC
ATCAATGAAA
ACCAGCAGTT
TGATGGCATG
GACTTTGAGC
AGGACAATGA
ATCCTGTACA
TGCCGGATTT
ACCGCAAGGA
CCGTAATCAT
CCGATCTGCG
TTACCGAATG
GATGGATGAA
TGCCGCCGCG
AACCATTCAA
AACTCGCGAA
GGCAGAGAAA
TCACGGGGCC
GTGGCAGTCG
CATCCCAAAC
GGATGTTACG
TCATAAAGCC
ATGATTCAGT
GTGCCCGTCT
GGCCTTCGGA
TTTGCTGGTA
TCTATGACAA
GGATGAAGCC
GAGCGCATTG
TCGAAAATAC
TGCATACACT
GCAGAACGTC
AGCCGGAACG
CGACATCACT
CCGGTTAACG
ATGAAACCAT
GCAGGAGATT
AACACTCTGC
TGATCGCCCT
GGATAAAACA
TGGGATGACG
ACTTATTGCC
GCTCTGTTCC
CAGATATTTC
GCCGCGACAT
TCGTGCATCG
TCAGAACTGA
CACAGGCCGA
AGCAGTAAAA
GCTCTTGGAT
TCCTGAAACA
GAAAGCCGCA
GAGCAGAAGG
TGGCAGCATG
ACACCGGACA
TTATCCTGCA
GCGTACCGGG
ATCGATGTGA
GAGCTGTCGA
ACAGGGGGAA
GATGCGTGGC
ACAAATTACG
GCTCGGCGTC
ATCACCGCTT
CAGAAGTTCA
CAACGTGATA
GCAAAACCCC
GCTCCGGAAA
GAAGTGGCCT
GACATGAAAA
TGTCCTACTT
CCACACCCTG
CTTGCTGAGG
TTTGCACCGG
TGTGGCTCCG
GAAGTTAACG
CTAAAGCACT
GGCCTGGGGA
AAACAGTACG
AGAACGACGC
CAGAACCCTG
TTTGAATTCA
CTTCCGGCGT
GAATGTTACT
GAATCCCCGA
TCATCTATCG
CGACGAAAGT
ATGCGTACCG
CCTGCTCTCC
CGATGGTTTA
TGCAGTGACG
GCAACGGCCT
TGAACTGAAA
TGCCCGTTTA
CCTCCCGGGA
TTTCATGAAG
TTCCGGCTCG
GTGGTTTCGA
GGCCATAAAG
TCAGCTTACA
TGGCCCAGGT
GCAGTACAGC
ATGTGGGTGA
CGCGAAAAAA
TGCCTGGTAC
TTTGCCAACT
ATGACCCGCG
TATGAAGCGT
GAAGGCCTGC
ATTATGTCGT
GATTGAGCGG
GATGAAAAGT
ACATGGCGAG
TTTTGACGAG
ATCGTGCCGG
AGTTCATCGA
AAAAATGGAC
GAGGCACTGG
CTGAAATTGG
TTTTGTATTT
GGGGAGCAAT
GGCGATGACG
CATCCTCACG
ATAATAAGCT
TCCTGCTGAA
CATCAAAGGC
AAGAAAACAT
CTGTTGTCAA
AGACAGCATC
CTTGAACAAG
GACAATTAAC
AGTTAACAAA
TAAAAACGCA
AAAGAAAATG
CCGATATTGA
CTACCGGAAG
CAGTGTGACC
GTGTGCTTCT
CAAATGCCTG
ATTCAGGCTG
TCTATGTGTG
ACTGTTGAGC
TGTAACAAGT
TGTCTCAGGT
GTTCAATTTC
ATGTTCTAGT
TGCTTTGTTT
TACTGGTTTC
ACCTGTTCTA
TTAGGTGTTA
CATGCTGTTC
ATCTGTTACA
TTGTCGATCT
GTTCATGGTG
AACAGCTTTA
AATGCACCAA
AAACTCGTAA
AAGCTCTGAT
GTATCTATCT
TTTTTACACC
GTTTTCATCT
GTGCATATAG
CTATACTGAT
TTCGTCAGAC
TCACAGTCAA
ACATGCCGGT
CAGTTGGCCT
GGTGATGGCG
GGATCGTTGT
ATATTTCTTG
ACACCTTTTC
GGCACCGCCC
TAAAATTCTG
CGTCCTCATA
ATATATGAGG
CGATTTATTA
CGTGTTTACG
AAGCAAAAGC
TAAAACCAGG
AGCTATTTAA
TGGATAAGAA
ATACTCAATA
GGCTTAGATA
TCGGCACAAA
TAGCGTCGGA
TGGGCGGTGA
TCACTGATGA
ATATAAGGTT
CCGTCTAAAA
AGTTCAAGGT
TCTGGGAAAT
ACAGACCGCC
ACAGTATCAA
AAAAAATCTT
ATAGGGGCTC
TTTTATTTGA
CAGTGGAGAG
ACAGCGGAAG
CGACTCGTCT
CAAACGGACA
GCTCGTAGAA
GGTATACACG
TCGGAAGAAT
CGTATTTGTT
ATCTACAGGA
GATTTTTTCA
AATGAGATGG
CGAAAGTAGA
TGATAGTTTC
TTTCATCGAC
TTGAAGAGTC
TTTTTTGGTG
GAAGAAGACA
AGAAGCATGA
ACGTCATCCT
ATTTTTGGAA
ATATAGTAGA
TGAAGTTGCT
TATCATGAGA
AATATCCAAC
TATCTATCAT
CTGCGAAAAA
AATTGGTAGA
TTCTACTGAT
AAAGCGGATT
TGCGCTTAAT
CTATTTGGCC
TTAGCGCATA
TGATTAAGTT
TCGTGGTCAT
TTTTTGATTG
AGGGAGATTT
AAATCCTGAT
AATAGTGATG
TGGACAAACT
ATTTATCCAG
TTGGTACAAA
CCTACAATCA
ATTATTTGAA
GAAAACCCTA
TTAACGCAAG
TGGAGTAGAT
GCTAAAGCGA
TTCTTTCTGC
ACGATTGAGT
AAATCAAGAC
GATTAGAAAA
TCTCATTGCT
CAGCTCCCCG
GTGAGAAGAA
AAATGGCTTA
TTTGGGAATC
TCATTGCTTT
GTCATTGGGT
TTGACCCCTA
ATTTTAAATC
AAATTTTGAT
TTGGCAGAAG
ATGCTAAATT
ACAGCTTTCA
AAAGATACTT
ACGATGATGA
TTTAGATAAT
TTATTGGCGC
AAATTGGAGA
TCAATATGCT
GATTTGTTTT
TGGCAGCTAA
GAATTTATCA
GATGCTATTT
TACTTTCAGA
TATCCTAAGA
GTAAATACTG
AAATAACTAA
GGCTCCCCTA
TCAGCTTCAA
TGATTAAACG
CTACGATGAA
CATCATCAAG
ACTTGACTCT
TTTAAAAGCT
TTAGTTCGAC
AACAACTTCC
AGAAAAGTAT
AAAGAAATCT
TTTTTGATCA
ATCAAAAAAC
GGATATGCAG
GTTATATTGA
TGGGGGAGCT
AGCCAAGAAG
AATTTTATAA
ATTTATCAAA
CCAATTTTAG
AAAAAATGGA
TGGTACTGAG
GAATTATTGG
TGAAACTAAA
TCGTGAAGAT
TTGCTGCGCA
AGCAACGGAC
CTTTGACAAC
GGCTCTATTC
CCCATCAAAT
TCACTTGGGT
GAGCTGCATG
CTATTTTGAG
AAGACAAGAA
GACTTTTATC
CATTTTTAAA
AGACAATCGT
GAGAAGATTG
AAAAAATCTT
GACTTTTCGA
ATTCCTTATT
ATGTTGGTCC
ATTGGCGCGT
GGCAATAGTC
GTTTTGCATG
GATGACTCGG
AAGTCTGAAG
AAACAATTAC
CCCATGGAAT
TTTGAAGAAG
TTGTCGATAA
AGGTGCTTCA
GCTCAATCAT
TTATTGAACG
CATGACAAAC
TTTGATAAAA
ATCTTCCAAA
TGAAAAAGTA
CTACCAAAAC
ATAGTTTGCT
TTATGAGTAT
TTTACGGTTT
ATAACGAATT
GACAAAGGTC
AAATATGTTA
CTGAAGGAAT
GCGAAAACCA
GCATTTCTTT
CAGGTGAACA
GAAGAAAGCC
ATTGTTGATT
TACTCTTCAA
AACAAATCGA
AAAGTAACCG
TTAAGCAATT
AAAAGAAGAT
TATTTCAAAA
AAATAGAATG
TTTTGATAGT
GTTGAAATTT
CAGGAGTTGA
AGATAGATTT
AATGCTTCAT
TAGGTACCTA
CCATGATTTG
CTAAAAATTA
TTAAAGATAA
AGATTTTTTG
GATAATGAAG
AAAATGAAGA
TATCTTAGAG
GATATTGTTT
TAACATTGAC
CTTATTTGAA
GATAGGGAGA
TGATTGAGGA
AAGACTTAAA
ACATATGCTC
ACCTCTTTGA
TGATAAGGTG
ATGAAACAGC
TTAAACGTCG
CCGTTATACT
GGTTGGGGAC
GTTTGTCTCG
AAAATTGATT
AATGGTATTA
GGGATAAGCA
ATCTGGCAAA
ACAATATTAG
ATTTTTTGAA
ATCAGATGGT
TTTGCCAATC
GCAATTTTAT
GCAGCTGATC
CATGATGATA
GTTTGACATT
TAAAGAAGAC
ATTCAAAAAG
CACAAGTGTC
TGGACAAGGC
GATAGTTTAC
ATGAACATAT
TGCAAATTTA
GCTGGTAGCC
CTGCTATTAA
AAAAGGTATT
TTACAGACTG
TAAAAGTTGT
TGATGAATTG
GTCAAAGTAA
TGGGGCGGCA
TAAGCCAGAA
AATATCGTTA
TTGAAATGGC
ACGTGAAAAT
CAGACAACTC
AAAAGGGCCA
GAAAAATTCG
CGAGAGCGTA
TGAAACGAAT
CGAAGAAGGT
ATCAAAGAAT
TAGGAAGTCA
GATTCTTAAA
GAGCATCCTG
TTGAAAATAC
TCAATTGCAA
AATGAAAAGC
TCTATCTCTA
TTATCTCCAA
AATGGAAGAG
ACATGTATGT
GGACCAAGAA
TTAGATATTA
ATCGTTTAAG
TGATTATGAT
GTCGATCACA
TTGTTCCACA
AAGTTTCCTT
AAAGACGATT
CAATAGACAA
TAAGGTCTTA
ACGCGTTCTG
ATAAAAATCG
TGGTAAATCG
GATAACGTTC
CAAGTGAAGA
AGTAGTCAAA
AAGATGAAAA
ACTATTGGAG
ACAACTTCTA
AACGCCAAGT
TAATCACTCA
ACGTAAGTTT
GATAATTTAA
CGAAAGCTGA
ACGTGGAGGT
TTGAGTGAAC
TTGATAAAGC
TGGTTTTATC
AAACGCCAAT
TGGTTGAAAC
TCGCCAAATC
ACTAAGCATG
TGGCACAAAT
TTTGGATAGT
CGCATGAATA
CTAAATACGA
TGAAAATGAT
AAACTTATTC
GAGAGGTTAA
AGTGATTACC
TTAAAATCTA
AATTAGTTTC
TGACTTCCGA
AAAGATTTCC
AATTCTATAA
AGTACGTGAG
ATTAACAATT
ACCATCATGC
CCATGATGCG
TATCTAAATG
CCGTCGTTGG
AACTGCTTTG
ATTAAGAAAT
ATCCAAAACT
TGAATCGGAG
TTTGTCTATG
GTGATTATAA
AGTTTATGAT
GTTCGTAAAA
TGATTGCTAA
GTCTGAGCAA
GAAATAGGCA
AAGCAACCGC
AAAATATTTC
TTTTACTCTA
ATATCATGAA
CTTCTTCAAA
ACAGAAATTA
CACTTGCAAA
TGGAGAGATT
CGCAAACGCC
CTCTAATCGA
AACTAATGGG
GAAACTGGAG
AAATTGTCTG
GGATAAAGGG
CGAGATTTTG
CCACAGTGCG
CAAAGTATTG
TCCATGCCCC
AAGTCAATAT
TGTCAAGAAA
ACAGAAGTAC
AGACAGGCGG
ATTCTCCAAG
GAGTCAATTT
TACCAAAAAG
AAATTCGGAC
AAGCTTATTG
CTCGTAAAAA
AGACTGGGAT
CCAAAAAAAT
ATGGTGGTTT
TGATAGTCCA
ACGGTAGCTT
ATTCAGTCCT
AGTGGTTGCT
AAGGTGGAAA
AAGGGAAATC
GAAGAAGTTA
AAATCCGTTA
AAGAGTTACT
AGGGATCACA
ATTATGGAAA
GAAGTTCCTT
TGAAAAAAAT
CCGATTGACT
TTTTAGAAGC
TAAAGGATAT
AAGGAAGTTA
AAAAAGACTT
AATCATTAAA
CTACCTAAAT
ATAGTCTTTT
TGAGTTAGAA
AACGGTCGTA
AACGGATGCT
GGCTAGTGCC
GGAGAATTAC
AAAAAGGAAA
TGAGCTGGCT
CTGCCAAGCA
AATATGTGAA
TTTTTTATAT
TTAGCTAGTC
ATTATGAAAA
GTTGAAGGGT
AGTCCAGAAG
ATAACGAACA
AAAACAATTG
TTTGTGGAGC
AGCATAAGCA
TTATTTAGAT
GAGATTATTG
AGCAAATCAG
TGAATTTTCT
AAGCGTGTTA
TTTTAGCAGA
TGCCAATTTA
GATAAAGTTC
TTAGTGCATA
TAACAAACAT
AGAGACAAAC
CAATACGTGA
ACAAGCAGAA
AATATTATTC
ATTTATTTAC
GTTGACGAAT
CTTGGAGCTC
CCGCTGCTTT
TAAATATTTT
GATACAACAA
TTGATCGTAA
ACGATATACG
TCTACAAAAG
AAGTTTTAGA
TGCCACTCTT
ATCCATCAAT
CCATCACTGG
TCTTTATGAA
ACACGCATTG
ATTTGAGTCA
GCTAGGAGGT
GACTGATGGC
TGGTTGGCGT
ACTGTTGTGG
TAAATACCCA
TATGGACAGT
TTTCCCTTTG
ATATGTAACG
GTGAACAGTT
GTTCTACTTT
TGTTTGTTAG
TCTTGATGCT
TCACTGATAG
ATACAAGAGC
CATAAGAACC
TCAGATCCTT
CCGTATTTAG
CCAGTATGTT
CTCTAGTGTG
GTTCGTTGTT
TTTGCGTGAG
CCATGAGAAC
GAACCATTGA
GATCATACTT
ACTTTGCATG
TCACTCAAAA
ATTTTGCCTC
AAAACTGGTG
AGCTGAATTT
TTGCAGTTAA
AGCATCGTGT
AGTGTTTTTC
TTAGTCCGTT
ATGTAGGTAG
GAATCTGATG
TAATGGTTGT
TGGTATTTTG
TCACCATTCA
TTTTTATCTG
GTTGTTCTCA
AGTTCGGTTA
CGAGATCCAT
TTGTCTATCT
AGTTCAACTT
GGAAAATCAA
CGTATCAGTC
GGGCGGCCTC
GCTTATCAAC
CACCAATTTC
ATATTGCTGT
AAGTGTTTAA
ATCTTTACTT
ATTGGTTTCA
AAACCCATTG
GTTAAGCCTT
TTAAACTCAT
GGTAGTTATT
TTCAAGCATT
AACATGAACT
TAAATTCATC
AAGGCTAATC
TCTATATTTG
CCTTGTGAGT
TTTCTTTTGT
GTTAGTTCTT
TTAATAACCA
CTCATAAATC
CTCATAGAGT
ATTTGTTTTC
AAAAGACTTA
ACATGTTCCA
GATTATATTT
TATGAATTTT
TTTAACTGGA
AAAGATAAGG
CAATATCTCT
TCACTAAAAA
CTAATTCTAA
TTTTTCGCTT
GAGAACTTGG
CATAGTTTGT
CCACTGGAAA
ATCTCAAAGC
CTTTAACCAA
AGGATTCCTG
ATTTCCACAG
TTCTCGTCAT
CAGCTCTCTG
GTTGCTTTAG
CTAATACACC
ATAAGCATTT
TCCCTACTGA
TGTTCATCAT
CTGAACGTAT
TGGTTATAAG
TGAACGATAC
CGTCCGTTCT
TTCCTTGTAG
GGTTTTCAAT
CGTGGGGTTG
AGTAGTGCCA
CACAGCATAA
AATTAGCTTG
GTTTCATGCT
CCGTTAAGTC
ATAGCGACTA
ATCGCTAGTT
CATTTGCTTT
GAAAACAACT
AATTCAGACA
TACATCTCAA
TTGGTCTAGG
TGATTTTAAT
CACTATACCA
ATTGAGATGG
GCTAGTCAAT
GATAATTACT
AGTCCTTTTC
CTTTGAGTTG
TGGGTATCTG
TAAATTCTGC
TAGACCTTTG
CTGGAAAACT
TGTAAATTCT
GCTAGACCCT
CTGTAAATTC
CGCTAGACCT
TTGTGTGTTT
TTTTTGTTTA
TATTCAAGTG
GTTATAATTT
ATAGAATAAA
GAAAGAATAA
AAAAAGATAA
AAAGAATAGA
TCCCAGCCCT
GTGTATAACT
CACTACTTTA
GTCAGTTCCG
CAGTATTACA
AAAGGATGTC
GCAAACGCTG
TTTGCTCCTC
TACAAAACAG
ACCTTAAAAC
CCTAAAGGCT
TAAGTAGCAC
CCTCGCAAGC
TCGGGCAAAT
CGCTGAATAT
TCCTTTTGTC
TCCGACCATC
AGGCACCTGA
GTCGCTGTCT
TTTTCGTGAC
ATTCAGTTCG
CTGCGCTCAC
GGCTCTGGCA
GTGAATGGGG
GTAAATGGCA
CTACAGGCGC
CTTTTATGGA
TTCATGCAAG
GAAACTACCC
ATAATACAAG
AAAAGCCCGT
CACGGGCTTC
TCAGGGCGTT
TTATGGCGGG
TCTGCTATGT
GGTGCTATCT
GACTTTTTGC
TGTTCAGCAG
TTCCTGCCCT
CTGATTTTCC
AGTCTGACCA
CTTCGGATTA
TCCCGTGACA
GGTCATTCAG
ACTGGCTAAT
GCACCCAGTA
AGGCAGCGGT
ATCATCAACA
GGCTTACCCG
TCTTACTGTC
GGGGATCGAC
GCTCTCCCTT
ATGCGACTCC
TGCACCTTTC
GTCTTCGAAT
AAATACCTGT
GACGGAAGAT
CACTTCGCAG
AATAAATAAA
TCCTGGTGTC
CCTGTTGATA
CCGGGAAGCC
CTGGGCCAAC
TTTTGGCGAA
AATGAGACGT
TGATCGGCAC
GTAAGAGGTT
CCAACTTTCA
CCATAATGAA
ATAAGATCAC
TACCGGGCGT
ATTTTTTGAG
TTATCGAGAT
TTTCAGGAGC
TAAGGAAGCT
AAAATGGAGA
AAAAAATCAC
TGGATATACC
ACCGTTGATA
TATCCCAATG
GCATCGTAAA
GAACATTTTG
AGGCATTTCA
GTCAGTTGAT
CAATGTACCT
ATAACCAGAC
CGTTCAGCTG
GGTCATCCAC
CGGATCAATT
CCCCTGCTCG
CGCAGGCTGG
GTGCCAAGCT
CTCGGGTAAC
ATCAAGGCCC
GATCCTTGGA
GCCCTTGCCC
TCCCGCACGA
TGATCGTGCC
GTGATCGAAA
TCCAGATCCT
TGACCCGCAG
TTGCAAACCC
TCACTGATCC
GGCTCACGGT
AACTGATGCC
GTATTTGCAG
TACCAGCGTA
CGGCCCACAG
AATGATGTCA
CGCTGAAAAT
GCCGGCCTTT
GAATGGGTTC
ATGTGCAGCT
CCATCAGCAA
AAGGGGATGA
TAAGTTTATC
ACCACCGACT
ATTTGCAACA
GTGCCGTTGA
TCGTGCTATG
ATCGACTGCC
TTAGAAGCAA
ACTTAAGAGT
GTGTTGATAG
TGCATTATCT
TAAAATTTTG
TATAATAGGA
ATTGAAGTTA
AATTAGATGC
TAAAAATAGG
AATTGAAGTT
AAATTAGATG
CTAAAAATTT
GTAATTAAGA
AGGAGGGATT
CGTCATGTTG
GTATTCCAAA
TGCGTAATGT
AGATAAAACA
TCTACTGTTT
TGAAACAGAC
TAAAAACAGT
GATTACGCAG
ATAAATAAAT
ACGTTAGATT
AATTCCTACC
AGTGACTAAT
CTTATGACTT
TTTAAACAGA
TAACTAAAAT
TACAAACAAA
TCGTTTAACT
TCAGGAGAGA
TTACATGAAC
AAAAATATAA
AATATTCTCA
AAACTTTTTA
ACGAGTGAAA
AAGTACTCAA
CCAAATAATA
AAACAATTGA
ATTTAAAAGA
AACCGATACC
GTTTACGAAA
TTGGAACAGG
TAAAGGGCAT
TTAACGACGA
AACTGGCTAA
AATAAGTAAA
CAGGTAACGT
CTATTGAATT
AGACAGTCAT
CTATTCAACT
TATCGTCAGA
AAAATTAAAA
CTGAATACTC
GTGTCACTTT
AATTCACCAA
GATATTCTAC
AGTTTCAATT
CCCTAACAAA
CAGAGGTATA
AAATTGTTGG
GAATATTCCT
TACAATTTAA
GCACACAAAT
TATTAAAAAA
GTGGTTTTTG
AAAGCCGTGC
GTCTGACATC
TATCTGACTG
TTGAAGAAGG
ATTCTACAAG
CGTACCTTGG
ATATTCACCG
AACACTAGGG
TTGCTCTTGC
ACACTCAAGT
CTCGATTCAG
CAATTGCTTA
AGCTGCCAGC
GGAATGCTTT
CATCCTAAAC
CAAAAGTAAA
CAGTGTCTTA
ATAAAACTTA
CCCGCCATAC
CACAGATGTT
CCAGATAAAT
ATTGGAAGCT
ATATAAGTAC
TTTGTTTCAA
AATGGGTCAA
TCGAGAATAT
CGTCAACTGT
TTACTAAAAA
TCAGTTTCGT
CAAGCAATGA
AACACGCCAA
AGTAAACAAT
TTAAGTACCA
TTACTTATGA
GCAAGTATTG
TCTATTTTTA
ATAGTTATCT
ATTATTTAAC
GGGAGGAAAT
AATGAGCTCA
TGAGCGGAGA
ACGAGATGAC
GTTGGAGGGG
CAAGGTCGCG
CTGATTGCTG
GGGCAACACG
TGAAAGGCGA
GATCACCAAG
GTAGTCGGCA
AATAATGTCT
AACGGTACCC
GGGGATCCTC
TAGAGTCGAC
CTGCATAATG
TGCCTGTCAA
ATGGACGAAG
CAGGGATTCT
GCAAACCCTA
TGCTACTCCG
TCAAGCCGTC
AATTGTCTGA
TTCGTTACCA
ATTATGACAA
CTTGACGGCT
ACATCATTCA
CTTTTTCTTC
ACAACCGGCA
CGGAACTCGC
TCGGGCTGGC
CCCGGTGCAT
TTTTTAAATA
CCCGCGAGAA
ATAGAGTTGA
TCGTCAAAAC
CAACATTGCG
ACCGACGGTG
GCGATAGGCA
TCCGGGTGGT
GCTCAAAAGC
AGCTTCGCCT
GGCTGATACG
TTGGTCCTCG
CGCCAGCTTA
AGACGCTAAT
CCCTAACTGC
TGGCGGAAAA
GATGTGACAG
ACGCGACGGC
GACAAGCAAA
CATGCTGTGC
GACGCTGGCG
ATATCAAAAT
TGCTGTCTGC
CAGGTGATCG
CTGATGTACT
GACAAGCCTC
GCGTACCCGA
TTATCCATCG
GTGGATGGAG
CGACTCGTTA
ATCGCTTCCA
TGCGCCGCAG
TAACAATTGC
TCAAGCAGAT
TTATCGCCAG
CAGCTCCGAA
TAGCGCCCTT
CCCCTTGCCC
GGCGTTAATG
ATTTGCCCAA
ACAGGTCGCT
GAAATGCGGC
TGGTGCGCTT
CATCCGGGCG
AAAGAACCCC
GTATTGGCAA
ATATTGACGG
CCAGTTAAGC
CATTCATGCC
AGTAGGCGCG
CGGACGAAAG
TAAACCCACT
GGTGATACCA
TTCGCGAGCC
TCCGGATGAC
GACCGTAGTG
ATGAATCTCT
CCTGGCGGGA
ACAGCAAAAT
ATCACCCGGT
CGGCAAACAA
ATTCTCGTCC
CTGATTTTTC
ACCACCCCCT
GACCGCGAAT
GGTGAGATTG
AGAATATAAC
CTTTCATTCC
CAGCGGTCGG
TCGATAAAAA
AATCGAGATA
ACCGTTGGCC
TCAATCGGCG
TTAAACCCGC
CACCAGATGG
GCATTAAACG
AGTATCCCGG
CAGCAGGGGA
TCATTTTGCG
CTTCAGCCAT
ACTTTTCATA
CTCCCGCCAT
TCAGAG