产品货号：P58323
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pLV-Tet-miniCMV-HLTF(human)-GFP-nanobody-mCherry-Puro |
|---|---|
| 别称: | NM_003071.4;HIP116; HIP116A; HLTF1; RNF80; SMARCA3;SNF2L3; ZBU1 |
| 基因长: | 3027bp |
| 质粒宿主： | 哺乳细胞,慢病毒 |
| 质粒用途： | 蛋白表达,四环素诱导 |
| 片段类型： | ORF |
| 片段物种： | 人 |
| 原核抗性： | Amp |
| 筛选标记： | Puro |
| 荧光标记： | 红色 |
| 启动子: | Tet-miniCMV |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | |
| 正向引物: | miniCMV-F |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
CCAAATGGCA
GTATTCATCC
ACAATTTTAA
AAGAAAAGGG
GGGATTGGGG
GGTACAGTGC
AGGGGAAAGA
ATAGTAGACA
TAATAGCAAC
AGACATACAA
ACTAAAGAAT
TACAAAAACA
AATTACAAAA
ATTCAAAATT
TTCGGGTTTA
TTACAGGGAC
AGCAGAGAGA
ATTCACGTGC
TGCAGGTCCG
AGGTTCTAGA
CGAGTTTACT
CCCTATCAGT
GATAGAGAAC
GATGTCGAGT
TTACTCCCTA
TCAGTGATAG
AGAACGTATG
TCGAGTTTAC
TCCCTATCAG
TGATAGAGAA
CGTATGTCGA
GTTTACTCCC
TATCAGTGAT
AGAGAACGTA
TGTCGAGTTT
ATCCCTATCA
GTGATAGAGA
ACGTATGTCG
AGTTTACTCC
CTATCAGTGA
TAGAGAACGT
ATGTCGAGGT
AGGCGTGTAC
GGTGGGAGGC
CTATATAAGC
AGAGCTCGTT
TAGTGAACCG
TCAGATCGCC
TCTGGCTAAC
TAGAGAACCC
ACTGCTTACT
GGCTTATCGA
AATTAATACG
ACTCACTATA
GGGAGACCCA
AGCTGTCAGA
TCTCGAGCTC
AAGCTTCGAA
TTCTTCACGC
CTAGCAGTGA
ACTCCAAGCC
ACCATGTCCT
GGATGTTCAA
GAGGGATCCA
GTTTGGAAGT
ACTTGCAGAC
TGTCCAGTAT
GGAGTTCATG
GAAATTTTCC
ACGCCTCTCA
TATCCAACTT
TCTTTCCACG
TTTTGAATTC
CAAGATGTTA
TCCCTCCAGA
TGACTTTCTA
ACTAGTGATG
AAGAAGTAGA
TTCCGTTTTA
TTTGGAAGTT
TGAGAGGTCA
TGTGGTTGGA
CTACGCTATT
ACACGGGAGT
AGTTAATAAT
AATGAAATGG
TTGCATTACA
ACGAGATCCT
AATAACCCTT
ATGATAAGAA
TGCAATTAAA
GTAAACAATG
TGAATGGAAA
TCAAGTTGGC
CATTTAAAGA
AAGAGCTTGC
AGGTGCTTTG
GCCTATATCA
TGGACAACAA
ATTGGCACAA
ATTGAAGGGG
TAGTTCCTTT
TGGTGCAAAC
AATGCTTTTA
CCATGCCTCT
GCATATGACT
TTTTGGGGAA
AAGAAGAAAA
TAGAAAAGCG
GTTTCAGATC
AGTTGAAGAA
ACATGGATTT
AAATTGGGTC
CTGCACCAAA
AACTTTAGGA
TTCAATTTGG
AAAGTGGTTG
GGGCTCTGGA
AGAGCTGGAC
CAAGCTATAG
TATGCCAGTG
CATGCTGCAG
TACAGATGAC
AACTGAACAG
CTTAAAACAG
AATTTGACAA
ATTGTTTGAA
GATTTAAAAG
AAGATGATAA
AACCCATGAA
ATGGAACCAG
CTGAGGCTAT
TGAAACACCA
CTGCTTCCAC
ATCAAAAACA
AGCTCTAGCT
TGGATGGTGT
CACGGGAAAA
TAGCAAAGAA
CTTCCACCAT
TCTGGGAACA
GCGAAATGAC
TTATACTATA
ACACAATAAC
AAATTTTTCT
GAGAAGGACC
GACCAGAAAA
TGTCCATGGA
GGAATTTTAG
CTGATGATAT
GGGTTTGGGT
AAAACTCTTA
CGGCCATTGC
AGTAATCCTT
ACCAACTTCC
ATGATGGCAG
ACCTCTTCCT
ATTGAAAGAG
TTAAAAAGAA
TCTACTGAAG
AAGGAATATA
ATGTTAACGA
TGACTCTATG
AAACTTGGAG
GAAACAATAC
CAGTGAAAAG
GCAGATGGAC
TAAGCAAAGA
CGCATCTAGA
TGTAGTGAAC
AACCCAGTAT
TTCAGATATC
AAGGAGAAGA
GTAAGTTTCG
CATGTCAGAA
TTGTCTAGCT
CCCGCCCCAA
AAGAAGAAAA
ACTGCTGTCC
AGTACATAGA
AAGCAGTGAT
TCAGAGGAAA
TTGAAACAAG
TGAATTGCCG
CAGAAAATGA
AAGGCAAACT
GAAAAATGTA
CAGTCTGAAA
CTAAAGGCAG
GGCGAAAGCA
GGATCTTCTA
AGGTTATAGA
AGATGTGGCA
TTTGCATGTG
CATTAACTTC
ATCTGTTCCT
ACAACAAAAA
AGAAAATGTT
GAAAAAGGGA
GCTTGTGCAG
TGGAGGGGTC
AAAGAAAACT
GATGTTGAGG
AGAGACCAAG
AACAACACTG
ATCATCTGTC
CGCTTTCTGT
GTTAAGCAAC
TGGATTGACC
AGTTTGGACA
ACATATAAAA
TCAGATGTAC
ACTTGAATTT
TTATGTTTAT
TATGGTCCTG
ATCGTATTAG
AGAACCGGCC
TTACTTTCAA
AACAGGATAT
TGTTTTGACT
ACGTATAATA
TTTTAACTCA
TGACTATGGA
ACTAAAGGAG
ATAGTCCATT
ACATAGCATA
AGGTGGCTAA
GAGTGATCCT
GGATGAAGGA
CATGCCATAC
GAAATCCAAA
TGCTCAGCAG
ACAAAAGCTG
TACTTGACTT
AGAATCAGAA
AGAAGATGGG
TTTTGACAGG
TACTCCAATC
CAGAATTCTT
TAAAGGACTT
GTGGTCTCTT
CTTTCCTTTT
TAAAACTTAA
ACCATTTATT
GATAGAGAAT
GGTGGCATAG
AACAATACAG
CGTCCTGTCA
CAATGGGAGA
TGAAGGAGGA
CTTAGGCGTT
TACAGTCCCT
AATTAAAAAT
ATTACACTTA
GAAGAACAAA
GACAAGCAAA
ATTAAAGGAA
AACCTGTTTT
GGAGTTACCA
GAACGTAAAG
TATTTATTCA
GCACATTACA
CTTTCAGATG
AAGAGAGAAA
GATTTATCAG
TCTGTGAAAA
ATGAAGGCAG
AGCCACTATT
GGAAGGTATT
TTAATGAAGG
GACTGTCCTG
GCACATTATG
CAGATGTCCT
GGGTCTTTTG
CTTAGACTGC
GGCAAATTTG
TTGCCATACT
TACCTTCTTA
CAAATGCAGT
GTCTTCCAAT
GGCCCCTCAG
GAAATGATAC
ACCTGAAGAA
CTGAGAAAGA
AGTTAATAAG
GAAGATGAAG
TTAATTCTGA
GCTCAGGTTC
AGATGAGGAA
TGTGCAATTT
GCCTGGATTC
TTTAACAGTT
CCTGTGATAA
CACATTGTGC
ACATGTATTT
TGTAAACCCT
GTATTTGCCA
AGTCATTCAG
AATGAGCAGC
CACATGCTAA
ATGCCCTTTA
TGCAGAAATG
ATATACATGA
AGATAATTTA
TTAGAATGTC
CTCCAGAAGA
ATTAGCACGT
GACAGTGAGA
AAAAGTCTGA
TATGGAATGG
ACATCCAGTT
CAAAGATTAA
TGCGCTAATG
CACGCATTGA
CTGACTTAAG
AAAGAAGAAT
CCCAACATAA
AAAGTTTGGT
TGTTTCTCAG
TTTACAACAT
TCCTGTCTTT
AATAGAAATA
CCACTTAAAG
CCTCTGGATT
TGTGTTTACT
CGTTTGGATG
GTTCCATGGC
CCAAAAGAAA
AGAGTTGAAT
CAATTCAGTG
TTTTCAAAAC
ACTGAAGCAG
GATCTCCAAC
TATAATGCTT
CTGTCCTTAA
AAGCAGGTGG
AGTTGGTTTG
AATCTGTCTG
CAGCTTCTCG
AGTGTTTTTA
ATGGATCCAG
CCTGGAATCC
TGCTGCTGAA
GATCAGTGCT
TTGACAGATG
CCATAGACTT
GGTCAGAAGC
AAGAAGTTAT
CATCACAAAA
TTCATTGTAA
AGGACTCTGT
TGAAGAAAAT
ATGCTGAAAA
TACAAAACAA
AAAGAGAGAA
CTTGCAGCAG
GAGCCTTTGG
AACTAAAAAA
CCAAATGCTG
ACGAAATGAA
ACAAGCCAAA
ATTAATGAAA
TCAGAACATT
AATTGACTTA
TACCCCTACG
ACGTGCCTGA
CTACGCCGGC
GGCGGTGGTT
CAGGCGGTGG
CGGTTCCGGT
GGTGGGGGAT
CAGTCCAACT
GGTGGAGTCT
GGTGGCGCTT
TGGTGCAGCC
AGGTGGCTCT
CTGCGTTTGT
CCTGTGCCGC
TTCTGGCTTC
CCAGTGAACC
GCTATTCCAT
GCGCTGGTAT
CGCCAGGCTC
CAGGCAAAGA
GCGTGAGTGG
GTAGCCGGTA
TGTCCAGCGC
GGGTGATCGT
AGCTCCTATG
AAGACTCCGT
GAAGGGCCGT
TTCACCATCA
GCCGTGACGA
TGCCCGTAAC
ACGGTGTATC
TGCAAATGAA
CAGCTTGAAA
CCTGAAGATA
CGGCCGTGTA
TTACTGTAAT
GTGAACGTGG
GCTTCGAGTA
TTGGGGCCAA
GGCACCCAGG
TCACCGTCTC
CAGCTAAGAT
CCTTGACTTG
CGGCCGCAAC
TCCCACCTGC
AACATGCGTG
ACTGACTGAG
GCCGCGACTC
TAGAGTCGAC
CTGCATCTAG
GCGCCGGAAT
TAGATCTCTC
GAGGTTAACG
AATTCTACCG
GGTAGGGGAG
GCGCTTTTCC
CAAGGCAGTC
TGGAGCATGC
GCTTTAGCAG
CCCCGCTGGG
CACTTGGCGC
TACACAAGTG
GCCTCTGGCC
TCGCACACAT
TCCACATCCA
CCGGTAGGCG
CCAACCGGCT
CCGTTCTTTG
GTGGCCCCTT
CGCGCCACCT
TCTACTCCTC
CCCTAGTCAG
GAAGTTCCCC
CCCGCCCCGC
AGCTCGCGTC
GTGCAGGACG
TGACAAATGG
AAGTAGCACG
TCTCACTAGT
CTCGTGCAGA
TGGACAGCAC
CGCTGAGCAA
TGGAAGCGGG
TAGGCCTTTG
GGGCAGCGGC
CAATAGCAGC
TTTGCTCCTT
CGCTTTCTGG
GCTCAGAGGC
TGGGAAGGGG
TGGGTCCGGG
GGCGGGCTCA
GGGGCGGGCT
CAGGGGCGGG
GCGGGCGCCC
GAAGGTCCTC
CGGAGGCCCG
GCATTCTGCA
CGCTTCAAAA
GCGCACGTCT
GCCGCGCTGT
TCTCCTCTTC
CTCATCTCCG
GGCCTTTCGA
CCTGCAGCCC
AAGCTTACCA
TGGTGAGCAA
GGGCGAGGAG
GATAACATGG
CCATCATCAA
GGAGTTCATG
CGCTTCAAGG
TGCACATGGA
GGGCTCCGTG
AACGGCCACG
AGTTCGAGAT
CGAGGGCGAG
GGCGAGGGCC
GCCCCTACGA
GGGCACCCAG
ACCGCCAAGC
TGAAGGTGAC
CAAGGGTGGC
CCCCTGCCCT
TCGCCTGGGA
CATCCTGTCC
CCTCAGTTCA
TGTACGGCTC
CAAGGCCTAC
GTGAAGCACC
CCGCCGACAT
CCCCGACTAC
TTGAAGCTGT
CCTTCCCCGA
GGGCTTCAAG
TGGGAGCGCG
TGATGAACTT
CGAGGACGGC
GGCGTGGTGA
CCGTGACCCA
GGACTCCTCC
CTGCAGGACG
GCGAGTTCAT
CTACAAGGTG
AAGCTGCGCG
GCACCAACTT
CCCCTCCGAC
GGCCCCGTAA
TGCAGAAGAA
GACCATGGGC
TGGGAGGCCT
CCTCCGAGCG
GATGTACCCC
GAGGACGGCG
CCCTGAAGGG
CGAGATCAAG
CAGAGGCTGA
AGCTGAAGGA
CGGCGGCCAC
TACGACGCTG
AGGTCAAGAC
CACCTACAAG
GCCAAGAAGC
CCGTGCAGCT
GCCCGGCGCC
TACAACGTCA
ACATCAAGTT
GGACATCACC
TCCCACAACG
AGGACTACAC
CATCGTGGAA
CAGTACGAAC
GCGCCGAGGG
CCGCCACTCC
ACCGGCGGCA
TGGACGAGCT
GTACAAGGCA
ACAAACTTCT
CTCTGCTGAA
ACAAGCCGGA
GATGTCGAAG
AGAATCCTGG
ACCGATGACC
GAGTACAAGC
CCACGGTGCG
CCTCGCCACC
CGCGACGACG
TCCCCAGGGC
CGTACGCACC
CTCGCCGCCG
CGTTCGCCGA
CTACCCCGCC
ACGCGCCACA
CCGTCGATCC
GGACCGCCAC
ATCGAGCGGG
TCACCGAGCT
GCAAGAACTC
TTCCTCACGC
GCGTCGGGCT
CGACATCGGC
AAGGTGTGGG
TCGCGGACGA
CGGCGCCGCG
GTGGCGGTCT
GGACCACGCC
GGAGAGCGTC
GAAGCGGGGG
CGGTGTTCGC
CGAGATCGGC
CCGCGCATGG
CCGAGTTGAG
CGGTTCCCGG
CTGGCCGCGC
AGCAACAGAT
GGAAGGCCTC
CTGGCGCCGC
ACCGGCCCAA
GGAGCCCGCG
TGGTTCCTGG
CCACCGTCGG
CGTCTCGCCC
GACCACCAGG
GCAAGGGTCT
GGGCAGCGCC
GTCGTGCTCC
CCGGAGTGGA
GGCGGCCGAG
CGCGCCGGGG
TGCCCGCCTT
CCTGGAGACC
TCCGCGCCCC
GCAACCTCCC
CTTCTACGAG
CGGCTCGGCT
TCACCGTCAC
CGCCGACGTC
GAGGTGCCCG
AAGGACCGCG
CACCTGGTGC
ATGACCCGCA
AGCCCGGTGC
CTGATCTTAT
CGATAATCAA
CCTCTGGATT
ACAAAATTTG
TGAAAGATTG
ACTGGTATTC
TTAACTATGT
TGCTCCTTTT
ACGCTATGTG
GATACGCTGC
TTTAATGCCT
TTGTATCATG
CTATTGCTTC
CCGTATGGCT
TTCATTTTCT
CCTCCTTGTA
TAAATCCTGG
TTGCTGTCTC
TTTATGAGGA
GTTGTGGCCC
GTTGTCAGGC
AACGTGGCGT
GGTGTGCACT
GTGTTTGCTG
ACGCAACCCC
CACTGGTTGG
GGCATTGCCA
CCACCTGTCA
GCTCCTTTCC
GGGACTTTCG
CTTTCCCCCT
CCCTATTGCC
ACGGCGGAAC
TCATCGCCGC
CTGCCTTGCC
CGCTGCTGGA
CAGGGGCTCG
GCTGTTGGGC
ACTGACAATT
CCGTGGTGTT
GTCGGGGAAA
TCATCGTCCT
TTCCTTGGCT
GCTCGCCTGT
GTTGCCACCT
GGATTCTGCG
CGGGACGTCC
TTCTGCTACG
TCCCTTCGGC
CCTCAATCCA
GCGGACCTTC
CTTCCCGCGG
CCTGCTGCCG
GCTCTGCGGC
CTCTTCCGCG
TCTTCGCCTT
CGCCCTCAGA
CGAGTCGGAT
CTCCCTTTGG
GCCGCCTCCC
CGCATCGATA
CCGTCGACCA
AGGCAGCTGT
AGATCTTAGC
CACTTTTTAA
AAGAAAAGGG
GGGACTGGAA
GGGCTAATTC
ACTCCCAACG
AAGACAAGAT
CTGCTTTTTG
CTTGTACTGG
GTCTCTCTGG
TTAGACCAGA
TCTGAGCCTG
GGAGCTCTCT
GGCTAACTAG
GGAACCCACT
GCTTAAGCCT
CAATAAAGCT
TGCCTTGAGT
GCTTCAAGTA
GTGTGTGCCC
GTCTGTTGTG
TGACTCTGGT
AACTAGAGAT
CCCTCAGACC
CTTTTAGTCA
GTGTGGAAAA
TCTCTAGCAG
CATCTAGAAT
TAATTCCGTG
TATTCTATAG
TGTCACCTAA
ATCGTATGTG
TATGATACAT
AAGGTTATGT
ATTAATTGTA
GCCGCGTTCT
AACGACAATA
TGTACAAGCC
TAATTGTGTA
GCATCTGGCT
TACTGAAGCA
GACCCTATCA
TCTCTCTCGT
AAACTGCCGT
CAGAGTCGGT
TTGGTTGGAC
GAACCTTCTG
AGTTTCTGGT
AACGCCGTCC
CGCACCCGGA
AATGGTCAGC
GAACCAATCA
GCAGGGTCAT
CGCTAGCCAG
ATCCTCTACG
CCGGACGCAT
CGTGGCCGGC
ATCACCGGCG
CCACAGGTGC
GGTTGCTGGC
GCCTATATCG
CCGACATCAC
CGATGGGGAA
GATCGGGCTC
GCCACTTCGG
GCTCATGAGC
GCTTGTTTCG
GCGTGGGTAT
GGTGGCAGGC
CCCGTGGCCG
GGGGACTGTT
GGGCGCCATC
TCCTTGCATG
CACCATTCCT
TGCGGCGGCG
GTGCTCAACG
GCCTCAACCT
ACTACTGGGC
TGCTTCCTAA
TGCAGGAGTC
GCATAAGGGA
GAGCGTCGAA
TGGTGCACTC
TCAGTACAAT
CTAGCTCTGA
TGCCGCATAG
TTAAGCCAGC
CCCGACACCC
GCCAACACCC
GCTGACGCGC
CCTGACGGGC
TTGTCTGCTC
CCGGCATCCG
CTTACAGACA
AGCTGTGACC
GTCTCCGGGA
GCTGCATGTG
TCAGAGGTTT
TCACCGTCAT
CACCGAAACG
CGCGAGACGA
AAGGGCCTCG
TGATACGCCT
ATTTTTATAG
GTTAATGTCA
TGATAATAAT
GGTTTCTTAG
ACGTCAGGTG
GCACTTTTCG
GGGAAATGTG
CGCGGAACCC
CTATTTGTTT
ATTTTTCTAA
ATACATTCAA
ATATGTATCC
GCTCATGAGA
CAATAACCCT
GATAAATGCT
TCAATAATAT
TGAAAAAGGA
AGAGTATGAG
TATTCAACAT
TTCCGTGTCG
CCCTTATTCC
CTTTTTTGCG
GCATTTTGCC
TTCCTGTTTT
TGCTCACCCA
GAAACGCTGG
TGAAAGTAAA
AGATGCTGAA
GATCAGTTGG
GTGCACGAGT
GGGTTACATC
GAACTGGATC
TCAACAGCGG
TAAGATCCTT
GAGAGTTTTC
GCCCCGAAGA
ACGTTTTCCA
ATGATGAGCA
CTTTTAAAGT
TCTGCTATGT
GGCGCGGTAT
TATCCCGTAT
TGACGCCGGG
CAAGAGCAAC
TCGGTCGCCG
CATACACTAT
TCTCAGAATG
ACTTGGTTGA
GTACTCACCA
GTCACAGAAA
AGCATCTTAC
GGATGGCATG
ACAGTAAGAG
AATTATGCAG
TGCTGCCATA
ACCATGAGTG
ATAACACTGC
GGCCAACTTA
CTTCTGACAA
CGATCGGAGG
ACCGAAGGAG
CTAACCGCTT
TTTTGCACAA
CATGGGGGAT
CATGTAACTC
GCCTTGATCG
TTGGGAACCG
GAGCTGAATG
AAGCCATACC
AAACGACGAG
CGTGACACCA
CGATGCCTGT
AGCAATGGCA
ACAACGTTGC
GCAAACTATT
AACTGGCGAA
CTACTTACTC
TAGCTTCCCG
GCAACAATTA
ATAGACTGGA
TGGAGGCGGA
TAAAGTTGCA
GGACCACTTC
TGCGCTCGGC
CCTTCCGGCT
GGCTGGTTTA
TTGCTGATAA
ATCTGGAGCC
GGTGAGCGTG
GGTCTCGCGG
TATCATTGCA
GCACTGGGGC
CAGATGGTAA
GCCCTCCCGT
ATCGTAGTTA
TCTACACGAC
GGGGAGTCAG
GCAACTATGG
ATGAACGAAA
TAGACAGATC
GCTGAGATAG
GTGCCTCACT
GATTAAGCAT
TGGTAACTGT
CAGACCAAGT
TTACTCATAT
ATACTTTAGA
TTGATTTAAA
ACTTCATTTT
TAATTTAAAA
GGATCTAGGT
GAAGATCCTT
TTTGATAATC
TCATGACCAA
AATCCCTTAA
CGTGAGTTTT
CGTTCCACTG
AGCGTCAGAC
CCCGTAGAAA
AGATCAAAGG
ATCTTCTTGA
GATCCTTTTT
TTCTGCGCGT
AATCTGCTGC
TTGCAAACAA
AAAAACCACC
GCTACCAGCG
GTGGTTTGTT
TGCCGGATCA
AGAGCTACCA
ACTCTTTTTC
CGAAGGTAAC
TGGCTTCAGC
AGAGCGCAGA
TACCAAATAC
TGTCCTTCTA
GTGTAGCCGT
AGTTAGGCCA
CCACTTCAAG
AACTCTGTAG
CACCGCCTAC
ATACCTCGCT
CTGCTAATCC
TGTTACCAGT
GGCTGCTGCC
AGTGGCGATA
AGTCGTGTCT
TACCGGGTTG
GACTCAAGAC
GATAGTTACC
GGATAAGGCG
CAGCGGTCGG
GCTGAACGGG
GGGTTCGTGC
ACACAGCCCA
GCTTGGAGCG
AACGACCTAC
ACCGAACTGA
GATACCTACA
GCGTGAGCTA
TGAGAAAGCG
CCACGCTTCC
CGAAGGGAGA
AAGGCGGACA
GGTATCCGGT
AAGCGGCAGG
GTCGGAACAG
GAGAGCGCAC
GAGGGAGCTT
CCAGGGGGAA
ACGCCTGGTA
TCTTTATAGT
CCTGTCGGGT
TTCGCCACCT
CTGACTTGAG
CGTCGATTTT
TGTGATGCTC
GTCAGGGGGG
CGGAGCCTAT
GGAAAAACGC
CAGCAACGCG
GCCTTTTTAC
GGTTCCTGGC
CTTTTGCTGG
CCTTTTGCTC
ACATGTTCTT
TCCTGCGTTA
TCCCCTGATT
CTGTGGATAA
CCGTATTACC
GCCTTTGAGT
GAGCTGATAC
CGCTCGCCGC
AGCCGAACGA
CCGAGCGCAG
CGAGTCAGTG
AGCGAGGAAG
CGGAAGAGCG
CCCAATACGC
AAACCGCCTC
TCCCCGCGCG
TTGGCCGATT
CATTAATGCA
GCTGTGGAAT
GTGTGTCAGT
TAGGGTGTGG
AAAGTCCCCA
GGCTCCCCAG
CAGGCAGAAG
TATGCAAAGC
ATGCATCTCA
ATTAGTCAGC
AACCAGGTGT
GGAAAGTCCC
CAGGCTCCCC
AGCAGGCAGA
AGTATGCAAA
GCATGCATCT
CAATTAGTCA
GCAACCATAG
TCCCGCCCCT
AACTCCGCCC
ATCCCGCCCC
TAACTCCGCC
CAGTTCCGCC
CATTCTCCGC
CCCATGGCTG
ACTAATTTTT
TTTATTTATG
CAGAGGCCGA
GGCCGCCTCG
GCCTCTGAGC
TATTCCAGAA
GTAGTGAGGA
GGCTTTTTTG
GAGGCCTAGG
CTTTTGCAAA
AAGCTTGGAC
ACAAGACAGG
CTTGCGAGAT
ATGTTTGAGA
ATACCACTTT
ATCCCGCGTC
AGGGAGAGGC
AGTGCGTAAA
AAGACGCGGA
CTCATGTGAA
ATACTGGTTT
TTAGTGCGCC
AGATCTCTAT
AATCTCGCGC
AACCTATTTT
CCCCTCGAAC
ACTTTTTAAG
CCGTAGATAA
ACAGGCTGGG
ACACTTCACA
TGAGCGAAAA
ATACATCGTC
ACCTGGGACA
TGTTGCAGAT
CCATGCACGT
AAACTCGCAA
GCCGACTGAT
GCCTTCTGAA
CAATGGAAAG
GCATTATTGC
CGTAAGCCGT
GGCGGTCTGT
ACCGGGTGCG
TTACTGGCGC
GTGAACTGGG
TATTCGTCAT
GTCGATACCG
TTTGTATTTC
CAGCTACGAT
CACGACAACC
AGCGCGAGCT
TAAAGTGCTG
AAACGCGCAG
AAGGCGATGG
CGAAGGCTTC
ATCGTTATTG
ATGACCTGGT
GGATACCGGT
GGTACTGCGG
TTGCGATTCG
TGAAATGTAT
CCAAAAGCGC
ACTTTGTCAC
CATCTTCGCA
AAACCGGCTG
GTCGTCCGCT
GGTTGATGAC
TATGTTGTTG
ATATCCCGCA
AGATACCTGG
ATTGAACAGC
CGTGGGATAT
GGGCGTCGTA
TTCGTCCCGC
CAATCTCCGG
TCGCTAATCT
TTTCAACGCC
TGGCACTGCC
GGGCGTTGTT
CTTTTTAACT
TCAGGCGGGT
TACAATAGTT
TCCAGTAAGT
ATTCTGGAGG
CTGCATCCAT
GACACAGGCA
AACCTGAGCG
AAACCCTGTT
CAAACCCCGC
TTTAAACATC
CTGAAACCTC
GACGCTAGTC
CGCCGCTTTA
ATCACGGCGC
ACAACCGCCT
GTGCAGTCGG
CCCTTGATGG
TAAAACCATC
CCTCACTGGT
ATCGCATGAT
TAACCGTCTG
ATGTGGATCT
GGCGCGGCAT
TGACCCACGC
GAAATCCTCG
ACGTCCAGGC
ACGTATTGTG
ATGAGCGATG
CCGAACGTAC
CGACGATGAT
TTATACGATA
CGGTGATTGG
CTACCGTGGC
GGCAACTGGA
TTTATGAGTG
GGCCCCGGAT
CTTTGTGAAG
GAACCTTACT
TCTGTGGTGT
GACATAATTG
GACAAACTAC
CTACAGAGAT
TTAAAGCTCT
AAGGTAAATA
TAAAATTTTT
AAGTGTATAA
TGTGTTAAAC
TACTGATTCT
AATTGTTTGT
GTATTTTAGA
TTCCAACCTA
TGGAACTGAT
GAATGGGAGC
AGTGGTGGAA
TGCCTTTAAT
GAGGAAAACC
TGTTTTGCTC
AGAAGAAATG
CCATCTAGTG
ATGATGAGGC
TACTGCTGAC
TCTCAACATT
CTACTCCTCC
AAAAAAGAAG
AGAAAGGTAG
AAGACCCCAA
GGACTTTCCT
TCAGAATTGC
TAAGTTTTTT
GAGTCATGCT
GTGTTTAGTA
ATAGAACTCT
TGCTTGCTTT
GCTATTTACA
CCACAAAGGA
AAAAGCTGCA
CTGCTATACA
AGAAAATTAT
GGAAAAATAT
TCTGTAACCT
TTATAAGTAG
GCATAACAGT
TATAATCATA
ACATACTGTT
TTTTCTTACT
CCACACAGGC
ATAGAGTGTC
TGCTATTAAT
AACTATGCTC
AAAAATTGTG
TACCTTTAGC
TTTTTAATTT
GTAAAGGGGT
TAATAAGGAA
TATTTGATGT
ATAGTGCCTT
GACTAGAGAT
CATAATCAGC
CATACCACAT
TTGTAGAGGT
TTTACTTGCT
TTAAAAAACC
TCCCACACCT
CCCCCTGAAC
CTGAAACATA
AAATGAATGC
AATTGTTGTT
GTTAACTTGT
TTATTGCAGC
TTATAATGGT
TACAAATAAA
GCAATAGCAT
CACAAATTTC
ACAAATAAAG
CATTTTTTTC
ACTGCATTCT
AGTTGTGGTT
TGTCCAAACT
CATCAATGTA
TCTTATCATG
TCTGGATCAA
CTGGATAACT
CAAGCTAACC
AAAATCATCC
CAAACTTCCC
ACCCCATACC
CTATTACCAC
TGCCAATTAC
CTAGTGGTTT
CATTTACTCT
AAACCTGTGA
TTCCTCTGAA
TTATTTTCAT
TTTAAAGAAA
TTGTATTTGT
TAAATATGTA
CTACAAACTT
AGTAGTTGGA
AGGGCTAATT
CACTCCCAAA
GAAGACAAGA
TATCCTTGAT
CTGTGGATCT
ACCACACACA
AGGCTACTTC
CCTGATTAGC
AGAACTACAC
ACCAGGGCCA
GGGGTCAGAT
ATCCACTGAC
CTTTGGATGG
TGCTACAAGC
TAGTACCAGT
TGAGCCAGAT
AAGGTAGAAG
AGGCCAATAA
AGGAGAGAAC
ACCAGCTTGT
TACACCCTGT
GAGCCTGCAT
GGGATGGATG
ACCCGGAGAG
AGAAGTGTTA
GAGTGGAGGT
TTGACAGCCG
CCTAGCATTT
CATCACGTGG
CCCGAGAGCT
GCATCCGGAG
TACTTCAAGA
ACTGCTGATA
TCGAGCTTGC
TACAAGGGAC
TTTCCGCTGG
GGACTTTCCA
GGGAGGCGTG
GCCTGGGCGG
GACTGGGGAG
TGGCGAGCCC
TCAGATCCTG
CATATAAGCA
GCTGCTTTTT
GCCTGTACTG
GGTCTCTCTG
GTTAGACCAG
ATCTGAGCCT
GGGAGCTCTC
TGGCTAACTA
GGGAACCCAC
TGCTTAAGCC
TCAATAAAGC
TTGCCTTGAG
TGCTTCAAGT
AGTGTGTGCC
CGTCTGTTGT
GTGACTCTGG
TAACTAGAGA
TCCCTCAGAC
CCTTTTAGTC
AGTGTGGAAA
ATCTCTAGCA
GTGGCGCCCG
AACAGGGACT
TGAAAGCGAA
AGGGAAACCA
GAGGAGCTCT
CTCGACGCAG
GACTCGGCTT
GCTGAAGCGC
GCACGGCAAG
AGGCGAGGGG
CGGCGACTGG
TGAGTACGCC
AAAAATTTTG
ACTAGCGGAG
GCTAGAAGGA
GAGAGATGGG
TGCGAGAGCG
TCAGTATTAA
GCGGGGGAGA
ATTAGATCGC
GATGGGAAAA
AATTCGGTTA
AGGCCAGGGG
GAAAGAAAAA
ATATAAATTA
AAACATATAG
TATGGGCAAG
CAGGGAGCTA
GAACGATTCG
CAGTTAATCC
TGGCCTGTTA
GAAACATCAG
AAGGCTGTAG
ACAAATACTG
GGACAGCTAC
AACCATCCCT
TCAGACAGGA
TCAGAAGAAC
TTAGATCATT
ATATAATACA
GTAGCAACCC
TCTATTGTGT
GCATCAAAGG
ATAGAGATAA
AAGACACCAA
GGAAGCTTTA
GACAAGATAG
AGGAAGAGCA
AAACAAAAGT
AAGACCACCG
CACAGCAAGC
GGCCGGTGAT
CTTCAGACCT
GGACGATATA
TATGAGGGAC
AATTGGAGAA
GTGAATTATA
TAAATATAAA
GTAGTAAAAA
TTGAACCATT
AGGAGTAGCA
CCCACCAAGG
CAAAGAGAAG
AGTGGTGCAG
AGAGAAAAAA
GAGCAGTGGG
AATAGGAGCT
TTGTTCCTTG
GGTTCTTGGG
AGCAGCAGGA
AGCACTATGG
GCGCAGCGTC
AATGACGCTG
ACGGTACAGG
CCAGACAATT
ATTGTCTGGT
ATAGTGCAGC
AGCAGAACAA
TTTGCTGAGG
GCTATTGAGG
CGCAACAGCA
TCTGTTGCAA
CTCACAGTCT
GGGGCATCAA
GCAGCTCCAG
GCAAGAATCC
TGGCTGTGGA
AAGATACCTA
AAGGATCAAC
AGCTCCTGGG
GATTTGGGGT
TGCTCTGGAA
AACTCATTTG
CACCACTGCT
GTGCCTTGGA
ATGCTAGTTG
GAGTAATAAA
TCTCTGGAAC
AGATTTGGAA
TCACACGACC
TGGATGGAGT
GGGACAGAGA
AATTAACAAT
TACACAAGCT
TAATACACTC
CTTAATTGAA
GAATCGCAAA
ACCAGCAAGA
AAAGAATGAA
CAAGAATTAT
TGGAATTAGA
TAAATGGGCA
AGTTTGTGGA
ATTGGTTTAA
CATAACAAAT
TGGCTGTGGT
ATATAAAATT
ATTCATAATG
ATAGTAGGAG
GCTTGGTAGG
TTTAAGAATA
GTTTTTGCTG
TACTTTCTAT
AGTGAATAGA
GTTAGGCAGG
GATATTCACC
ATTATCGTTT
CAGACCCACC
TCCCAACCCC
GAGGGGACCC
GACAGGCCCG
AAGGAATAGA
AGAAGAAGGT
GGAGAGAGAG
ACAGAGACAG
ATCCATTCGA
TTAGTGAACG
GATCTCGACG
GTCG
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