产品货号：P58294
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pLV-Tet-miniCMV-BTBD7(human)-GFP-nanobody-mCherry-Puro |
|---|---|
| 别称: | NM_001002860.3;FUP1 |
| 基因长: | 3396bp |
| 质粒宿主： | 哺乳细胞,慢病毒 |
| 质粒用途： | 蛋白表达,四环素诱导 |
| 片段类型： | ORF |
| 片段物种： | 人 |
| 原核抗性： | Amp |
| 筛选标记： | Puro |
| 荧光标记： | 红色 |
| 启动子: | Tet-miniCMV |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | Stbl3 |
| 温度: | 37度 |
| 背景载体: | |
| 正向引物: | miniCMV-F |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
CCAAATGGCA
GTATTCATCC
ACAATTTTAA
AAGAAAAGGG
GGGATTGGGG
GGTACAGTGC
AGGGGAAAGA
ATAGTAGACA
TAATAGCAAC
AGACATACAA
ACTAAAGAAT
TACAAAAACA
AATTACAAAA
ATTCAAAATT
TTCGGGTTTA
TTACAGGGAC
AGCAGAGAGA
ATTCACGTGC
TGCAGGTCCG
AGGTTCTAGA
CGAGTTTACT
CCCTATCAGT
GATAGAGAAC
GATGTCGAGT
TTACTCCCTA
TCAGTGATAG
AGAACGTATG
TCGAGTTTAC
TCCCTATCAG
TGATAGAGAA
CGTATGTCGA
GTTTACTCCC
TATCAGTGAT
AGAGAACGTA
TGTCGAGTTT
ATCCCTATCA
GTGATAGAGA
ACGTATGTCG
AGTTTACTCC
CTATCAGTGA
TAGAGAACGT
ATGTCGAGGT
AGGCGTGTAC
GGTGGGAGGC
CTATATAAGC
AGAGCTCGTT
TAGTGAACCG
TCAGATCGCC
TCTGGCTAAC
TAGAGAACCC
ACTGCTTACT
GGCTTATCGA
AATTAATACG
ACTCACTATA
GGGAGACCCA
AGCTGTCAGA
TCTCGAGCTC
AAGCTTCGAA
TTCTTTTCCC
GGAGCAGTGA
ACTCCAAGCC
ACCATGGGTG
CTAATGCATC
TAATTATCCT
CATTCATGTT
CCCCGAGGGT
AGGGGGAAAT
TCACAGGCCC
AACAGACTTT
TATAGGGACC
TCATCCTATT
CTCAGCAAGG
CTATGGTTGC
GAATCAAAGT
TGTATAGCCT
TGACCATGGC
CATGAGAAAC
CACAAGACAA
AAAAAAGAGA
ACCTCTGGTC
TTGCCACCCT
CAAAAAGAAG
TTTATTAAGC
GTCGGAAATC
TAATAGGTCT
GCCGATCATG
CCAAGCAGAT
GCGAGAACTC
CTCTCTGGGT
GGGATGTTAG
AGATGTCAAT
GCATTAGTGG
AGGAATATGA
GGGAACATCA
GCATTAAAGG
AGCTTTCTCT
ACAAGCCAGT
TTGGCTAGAC
CAGAAGCCCG
GACATTGCAG
AAAGATATGG
CTGATCTTTA
TGAGTACAAG
TATTGTACTG
ATGTAGACTT
AATATTTCAA
GAAACTTGTT
TTCCTGTTCA
TCGTGCCATT
TTGGCAGCAA
GGTGTCCATT
TTTTAAAACA
CTGCTTTCTT
CCTCACCAGA
GTATGGGGCA
GAGATAATAA
TGGACATCAA
TACAGCTGGT
ATTGATATGC
CCATGTTTTC
TGCTTTGTTA
CACTACCTTT
ATACAGGAGA
GTTTGGAATG
GAGGACTCAA
GGTTTCAAAA
TGTCGATATC
CTTGTTCAGC
TTAGTGAAGA
ATTTGGAACA
CCAAATTCCC
TTGATGTAGA
TATGCGTGGA
CTCTTTGATT
ACATGTGTTA
TTATGATGTC
GTCCTTAGTT
TTTCTTCAGA
CTCTGAACTG
GTTGAAGCTT
TTGGTGGAAA
TCAGAACTGT
TTAGATGAAG
AGCTCAAAGC
CCACAAGGCT
GTTATTTCTG
CACGGTCCCC
ATTTTTTCGA
AATTTATTAC
AAAGGAGGAT
ACGAACTGGT
GAAGAAATCA
CAGACCGAAC
TTTGAGGACT
CCCACAAGAA
TTATATTAGA
TGAGTCCATT
ATACCAAAAA
AATATGCAAC
AGTGATATTA
CACTGTATGT
ATACCGACGT
GGTGGACCTC
TCTGTTTTGC
ACTGTAGCCC
CTCTGTGGGG
AGTCTCAGTG
AAGTTCAGGC
TCTCGTCGCA
GGGAAGCCAA
ACATGACCAG
GGCAGAAGAA
GCCATGGAAC
TTTACCACAT
AGCACTGTTC
TTGGAATTTA
ACATGCTTGC
ACAAGGCTGT
GAGGATATCA
TTGCTGAGAG
CATCTCATTA
GATACCTTAA
TTGCCATCCT
CAAGTGGAGT
TCTCATCCAT
ATGGCTCTAA
ATGGGTGCAC
CGACAAGCTT
TACATTTCCT
CTGTGAGGAA
TTTTCCCAGG
TCATGACTTC
GGATGTTTTT
TATGAACTCA
GCAAAGACCA
TCTGCTTACT
GCTATCCAGT
CTGACTACCT
ACAGGCAAGT
GAACAAGATA
TCCTTAAATA
TCTGATTAAA
TGGGGAGAGC
ATCAGTTGAT
GAAAAGAATA
GCAGATAGAG
AGCCAAACTT
ACTGAGTGGC
ACTGCCCATA
GTGTGAACAA
AAGAGGTGTA
AAAAGACGGG
ACCTGGACAT
GGAAGAGCTC
AGAGAGATCC
TTTCTTCTCT
CTTACCTTTT
GTGCGAATTG
AACACATCTT
ACCTATAAAC
AGTGAAGTCT
TAAGTGATGC
AATGAAAAGA
GGCTTGATTA
GTACTCCTCC
ATCAGATATG
CTTCCTACAA
CAGAAGGTGG
GAAGTCAAAT
GCCTGGTTAC
GGCAAAAAAA
TGCTGGCATC
TATGTTCGTC
CTCGACTCTT
CTCTCCCTAT
GTGGAAGAAG
CAAAGTCAGT
GCTAGATGAG
ATGATGGTGG
AACAAACGGA
TCTTGTGCGC
TTGCGAATGG
TTAGAATGTC
CAATGTGCCA
GACACGCTCT
ACATGGTCAA
TAATGCCGTG
CCACAGTGTT
GTCACATGAT
CAGCCACCAG
CAGATCAGCA
GCAACCAGTC
AAGCCCTCCT
TCAGTTGTAG
CCAACGAAAT
TCCAGTTCCT
CGTCTCCTCA
TTATGAAAGA
CATGGTCAGA
CGACTGCAGG
AACTGCGGCA
CACGGAGCAG
GTGCAGAGGG
CCTATGCCCT
GAACTGCGGG
GAAGGCGCCA
CTGTCAGCTA
TGAAATTCAG
ATTCGAGTGC
TAAGAGAGTT
TGGTCTTGCA
GATGCTGCTG
CAGAGCTGTT
GCAGAATCCT
CACAAATTCT
TTCCTGATGA
ACGTTTTGGG
GATGAAAGTC
CACTCTTGAC
AATGAGACAG
CCTGGGAGAT
GTCGCGTAAA
CAGTACACCT
CCTGCAGAAA
CCATGTTTAC
AGATCTGGAC
TCTTTTGTGG
CCTTCCATCC
ACCCTTGCCC
CCTCCACCAC
CTCCCTACCA
CCCCCCAGCT
ACCCCAATCC
ATAACCAACT
CAAAGCAGGC
TGGAAGCAAA
GACCTCCCAG
TCAGCACCCT
TCACGTTCAT
TTTCTTATCC
CTGTAATCAT
TCGCTGTTCC
ACTCCAGAAC
AGCTCCTAAA
GCTGGCCCTC
CCCCAGTCTA
CTTGCCGAGT
GTGAAAGCTG
CACCGCCTGA
TTGTACCAGC
ACTGCAGGAC
TGGGCAGACA
GACGGTGGCT
GCTGCTGCCG
CCACCACCAC
CTCAACAGCA
ACAGCAGCAG
CAGCAGCAGC
ATCTGAGAAG
CAAGTGCGAA
CACAACCTGT
GCTGAATGAT
CTGATGCCAG
ACATCGCGGT
GGGTGTGTCC
ACACTGTCAC
TCAAGGACAG
GAGGCTTCCA
GAGCTTGCTG
TAGACACAGA
ATTAAGCCAG
TCAGTTTCTG
AAGCAGGACC
AGGGCCTCCC
CAGCATCTGT
CGTGTATTCC
ACAGAGACAT
ACACACACTT
CTCGGAAAAA
ACACACACTA
GAGCAAAAAA
CAGACACCAG
AGAAAATCCA
CAGGAATATC
CGGATTTCTA
TGACTTCTCA
AATGCTGCTT
GCAGACCTTC
TACTCCTGCT
CTCAGCAGAC
GCACCCCTTC
CCCTTCGCAA
GGTGGATATT
TTGGTCCCGA
TCTGTACAGC
CACAATAAGG
CATCACCAAG
TGGCTTAAAG
TCAGCCTACC
TACCTGGTCA
GACGTCTCCT
AAAAAACAGG
AAGAAGCTAG
GAGAGAATAT
CCACTTTCCC
CTGACGGGCA
TCTACACAGA
CAAAAGAATG
AGCCGATACA
CCTGGATGTC
GTTGAGCAAC
CTCCCCAGCG
GTCAGACTTT
CCTTTGGCAG
CCCCAGAAAA
TGCTAGTACC
GGTCCAGCCC
ATGTCAGGGG
ACGAACTGCA
GTAGAAACTG
ACTTGACTTT
TGGGCTGACT
CCTAACAGAC
CTTCACTTTC
TGCATGTAGC
TCTGAAGCTC
CCGAAGAGAG
ATCCGGTAGA
AGACTGGCAG
ACAGTGAGTC
CCTGGGCCAT
GGAGCTCAGA
GAAATACAGA
TTTGGAAAGG
GAAGATTCAA
TAAGCAGAGG
AAGGAGGTCA
CCAAGCAAGC
CGGACTTCCT
CTACAAAAAG
TCTGCCCTCT
ACCCCTACGA
CGTGCCTGAC
TACGCCGGCG
GCGGTGGTTC
AGGCGGTGGC
GGTTCCGGTG
GTGGGGGATC
AGTCCAACTG
GTGGAGTCTG
GTGGCGCTTT
GGTGCAGCCA
GGTGGCTCTC
TGCGTTTGTC
CTGTGCCGCT
TCTGGCTTCC
CAGTGAACCG
CTATTCCATG
CGCTGGTATC
GCCAGGCTCC
AGGCAAAGAG
CGTGAGTGGG
TAGCCGGTAT
GTCCAGCGCG
GGTGATCGTA
GCTCCTATGA
AGACTCCGTG
AAGGGCCGTT
TCACCATCAG
CCGTGACGAT
GCCCGTAACA
CGGTGTATCT
GCAAATGAAC
AGCTTGAAAC
CTGAAGATAC
GGCCGTGTAT
TACTGTAATG
TGAACGTGGG
CTTCGAGTAT
TGGGGCCAAG
GCACCCAGGT
CACCGTCTCC
AGCTAAGATC
CTTGACTTGC
GGCCGCAACT
CCCACCTGCA
ACATGCGTGA
CTGACTGAGG
CCGCGACTCT
AGAGTCGACC
TGCATCTAGG
CGCCGGAATT
AGATCTCTCG
AGGTTAACGA
ATTCTACCGG
GTAGGGGAGG
CGCTTTTCCC
AAGGCAGTCT
GGAGCATGCG
CTTTAGCAGC
CCCGCTGGGC
ACTTGGCGCT
ACACAAGTGG
CCTCTGGCCT
CGCACACATT
CCACATCCAC
CGGTAGGCGC
CAACCGGCTC
CGTTCTTTGG
TGGCCCCTTC
GCGCCACCTT
CTACTCCTCC
CCTAGTCAGG
AAGTTCCCCC
CCGCCCCGCA
GCTCGCGTCG
TGCAGGACGT
GACAAATGGA
AGTAGCACGT
CTCACTAGTC
TCGTGCAGAT
GGACAGCACC
GCTGAGCAAT
GGAAGCGGGT
AGGCCTTTGG
GGCAGCGGCC
AATAGCAGCT
TTGCTCCTTC
GCTTTCTGGG
CTCAGAGGCT
GGGAAGGGGT
GGGTCCGGGG
GCGGGCTCAG
GGGCGGGCTC
AGGGGCGGGG
CGGGCGCCCG
AAGGTCCTCC
GGAGGCCCGG
CATTCTGCAC
GCTTCAAAAG
CGCACGTCTG
CCGCGCTGTT
CTCCTCTTCC
TCATCTCCGG
GCCTTTCGAC
CTGCAGCCCA
AGCTTACCAT
GGTGAGCAAG
GGCGAGGAGG
ATAACATGGC
CATCATCAAG
GAGTTCATGC
GCTTCAAGGT
GCACATGGAG
GGCTCCGTGA
ACGGCCACGA
GTTCGAGATC
GAGGGCGAGG
GCGAGGGCCG
CCCCTACGAG
GGCACCCAGA
CCGCCAAGCT
GAAGGTGACC
AAGGGTGGCC
CCCTGCCCTT
CGCCTGGGAC
ATCCTGTCCC
CTCAGTTCAT
GTACGGCTCC
AAGGCCTACG
TGAAGCACCC
CGCCGACATC
CCCGACTACT
TGAAGCTGTC
CTTCCCCGAG
GGCTTCAAGT
GGGAGCGCGT
GATGAACTTC
GAGGACGGCG
GCGTGGTGAC
CGTGACCCAG
GACTCCTCCC
TGCAGGACGG
CGAGTTCATC
TACAAGGTGA
AGCTGCGCGG
CACCAACTTC
CCCTCCGACG
GCCCCGTAAT
GCAGAAGAAG
ACCATGGGCT
GGGAGGCCTC
CTCCGAGCGG
ATGTACCCCG
AGGACGGCGC
CCTGAAGGGC
GAGATCAAGC
AGAGGCTGAA
GCTGAAGGAC
GGCGGCCACT
ACGACGCTGA
GGTCAAGACC
ACCTACAAGG
CCAAGAAGCC
CGTGCAGCTG
CCCGGCGCCT
ACAACGTCAA
CATCAAGTTG
GACATCACCT
CCCACAACGA
GGACTACACC
ATCGTGGAAC
AGTACGAACG
CGCCGAGGGC
CGCCACTCCA
CCGGCGGCAT
GGACGAGCTG
TACAAGGCAA
CAAACTTCTC
TCTGCTGAAA
CAAGCCGGAG
ATGTCGAAGA
GAATCCTGGA
CCGATGACCG
AGTACAAGCC
CACGGTGCGC
CTCGCCACCC
GCGACGACGT
CCCCAGGGCC
GTACGCACCC
TCGCCGCCGC
GTTCGCCGAC
TACCCCGCCA
CGCGCCACAC
CGTCGATCCG
GACCGCCACA
TCGAGCGGGT
CACCGAGCTG
CAAGAACTCT
TCCTCACGCG
CGTCGGGCTC
GACATCGGCA
AGGTGTGGGT
CGCGGACGAC
GGCGCCGCGG
TGGCGGTCTG
GACCACGCCG
GAGAGCGTCG
AAGCGGGGGC
GGTGTTCGCC
GAGATCGGCC
CGCGCATGGC
CGAGTTGAGC
GGTTCCCGGC
TGGCCGCGCA
GCAACAGATG
GAAGGCCTCC
TGGCGCCGCA
CCGGCCCAAG
GAGCCCGCGT
GGTTCCTGGC
CACCGTCGGC
GTCTCGCCCG
ACCACCAGGG
CAAGGGTCTG
GGCAGCGCCG
TCGTGCTCCC
CGGAGTGGAG
GCGGCCGAGC
GCGCCGGGGT
GCCCGCCTTC
CTGGAGACCT
CCGCGCCCCG
CAACCTCCCC
TTCTACGAGC
GGCTCGGCTT
CACCGTCACC
GCCGACGTCG
AGGTGCCCGA
AGGACCGCGC
ACCTGGTGCA
TGACCCGCAA
GCCCGGTGCC
TGATCTTATC
GATAATCAAC
CTCTGGATTA
CAAAATTTGT
GAAAGATTGA
CTGGTATTCT
TAACTATGTT
GCTCCTTTTA
CGCTATGTGG
ATACGCTGCT
TTAATGCCTT
TGTATCATGC
TATTGCTTCC
CGTATGGCTT
TCATTTTCTC
CTCCTTGTAT
AAATCCTGGT
TGCTGTCTCT
TTATGAGGAG
TTGTGGCCCG
TTGTCAGGCA
ACGTGGCGTG
GTGTGCACTG
TGTTTGCTGA
CGCAACCCCC
ACTGGTTGGG
GCATTGCCAC
CACCTGTCAG
CTCCTTTCCG
GGACTTTCGC
TTTCCCCCTC
CCTATTGCCA
CGGCGGAACT
CATCGCCGCC
TGCCTTGCCC
GCTGCTGGAC
AGGGGCTCGG
CTGTTGGGCA
CTGACAATTC
CGTGGTGTTG
TCGGGGAAAT
CATCGTCCTT
TCCTTGGCTG
CTCGCCTGTG
TTGCCACCTG
GATTCTGCGC
GGGACGTCCT
TCTGCTACGT
CCCTTCGGCC
CTCAATCCAG
CGGACCTTCC
TTCCCGCGGC
CTGCTGCCGG
CTCTGCGGCC
TCTTCCGCGT
CTTCGCCTTC
GCCCTCAGAC
GAGTCGGATC
TCCCTTTGGG
CCGCCTCCCC
GCATCGATAC
CGTCGACCAA
GGCAGCTGTA
GATCTTAGCC
ACTTTTTAAA
AGAAAAGGGG
GGACTGGAAG
GGCTAATTCA
CTCCCAACGA
AGACAAGATC
TGCTTTTTGC
TTGTACTGGG
TCTCTCTGGT
TAGACCAGAT
CTGAGCCTGG
GAGCTCTCTG
GCTAACTAGG
GAACCCACTG
CTTAAGCCTC
AATAAAGCTT
GCCTTGAGTG
CTTCAAGTAG
TGTGTGCCCG
TCTGTTGTGT
GACTCTGGTA
ACTAGAGATC
CCTCAGACCC
TTTTAGTCAG
TGTGGAAAAT
CTCTAGCAGC
ATCTAGAATT
AATTCCGTGT
ATTCTATAGT
GTCACCTAAA
TCGTATGTGT
ATGATACATA
AGGTTATGTA
TTAATTGTAG
CCGCGTTCTA
ACGACAATAT
GTACAAGCCT
AATTGTGTAG
CATCTGGCTT
ACTGAAGCAG
ACCCTATCAT
CTCTCTCGTA
AACTGCCGTC
AGAGTCGGTT
TGGTTGGACG
AACCTTCTGA
GTTTCTGGTA
ACGCCGTCCC
GCACCCGGAA
ATGGTCAGCG
AACCAATCAG
CAGGGTCATC
GCTAGCCAGA
TCCTCTACGC
CGGACGCATC
GTGGCCGGCA
TCACCGGCGC
CACAGGTGCG
GTTGCTGGCG
CCTATATCGC
CGACATCACC
GATGGGGAAG
ATCGGGCTCG
CCACTTCGGG
CTCATGAGCG
CTTGTTTCGG
CGTGGGTATG
GTGGCAGGCC
CCGTGGCCGG
GGGACTGTTG
GGCGCCATCT
CCTTGCATGC
ACCATTCCTT
GCGGCGGCGG
TGCTCAACGG
CCTCAACCTA
CTACTGGGCT
GCTTCCTAAT
GCAGGAGTCG
CATAAGGGAG
AGCGTCGAAT
GGTGCACTCT
CAGTACAATC
TAGCTCTGAT
GCCGCATAGT
TAAGCCAGCC
CCGACACCCG
CCAACACCCG
CTGACGCGCC
CTGACGGGCT
TGTCTGCTCC
CGGCATCCGC
TTACAGACAA
GCTGTGACCG
TCTCCGGGAG
CTGCATGTGT
CAGAGGTTTT
CACCGTCATC
ACCGAAACGC
GCGAGACGAA
AGGGCCTCGT
GATACGCCTA
TTTTTATAGG
TTAATGTCAT
GATAATAATG
GTTTCTTAGA
CGTCAGGTGG
CACTTTTCGG
GGAAATGTGC
GCGGAACCCC
TATTTGTTTA
TTTTTCTAAA
TACATTCAAA
TATGTATCCG
CTCATGAGAC
AATAACCCTG
ATAAATGCTT
CAATAATATT
GAAAAAGGAA
GAGTATGAGT
ATTCAACATT
TCCGTGTCGC
CCTTATTCCC
TTTTTTGCGG
CATTTTGCCT
TCCTGTTTTT
GCTCACCCAG
AAACGCTGGT
GAAAGTAAAA
GATGCTGAAG
ATCAGTTGGG
TGCACGAGTG
GGTTACATCG
AACTGGATCT
CAACAGCGGT
AAGATCCTTG
AGAGTTTTCG
CCCCGAAGAA
CGTTTTCCAA
TGATGAGCAC
TTTTAAAGTT
CTGCTATGTG
GCGCGGTATT
ATCCCGTATT
GACGCCGGGC
AAGAGCAACT
CGGTCGCCGC
ATACACTATT
CTCAGAATGA
CTTGGTTGAG
TACTCACCAG
TCACAGAAAA
GCATCTTACG
GATGGCATGA
CAGTAAGAGA
ATTATGCAGT
GCTGCCATAA
CCATGAGTGA
TAACACTGCG
GCCAACTTAC
TTCTGACAAC
GATCGGAGGA
CCGAAGGAGC
TAACCGCTTT
TTTGCACAAC
ATGGGGGATC
ATGTAACTCG
CCTTGATCGT
TGGGAACCGG
AGCTGAATGA
AGCCATACCA
AACGACGAGC
GTGACACCAC
GATGCCTGTA
GCAATGGCAA
CAACGTTGCG
CAAACTATTA
ACTGGCGAAC
TACTTACTCT
AGCTTCCCGG
CAACAATTAA
TAGACTGGAT
GGAGGCGGAT
AAAGTTGCAG
GACCACTTCT
GCGCTCGGCC
CTTCCGGCTG
GCTGGTTTAT
TGCTGATAAA
TCTGGAGCCG
GTGAGCGTGG
GTCTCGCGGT
ATCATTGCAG
CACTGGGGCC
AGATGGTAAG
CCCTCCCGTA
TCGTAGTTAT
CTACACGACG
GGGAGTCAGG
CAACTATGGA
TGAACGAAAT
AGACAGATCG
CTGAGATAGG
TGCCTCACTG
ATTAAGCATT
GGTAACTGTC
AGACCAAGTT
TACTCATATA
TACTTTAGAT
TGATTTAAAA
CTTCATTTTT
AATTTAAAAG
GATCTAGGTG
AAGATCCTTT
TTGATAATCT
CATGACCAAA
ATCCCTTAAC
GTGAGTTTTC
GTTCCACTGA
GCGTCAGACC
CCGTAGAAAA
GATCAAAGGA
TCTTCTTGAG
ATCCTTTTTT
TCTGCGCGTA
ATCTGCTGCT
TGCAAACAAA
AAAACCACCG
CTACCAGCGG
TGGTTTGTTT
GCCGGATCAA
GAGCTACCAA
CTCTTTTTCC
GAAGGTAACT
GGCTTCAGCA
GAGCGCAGAT
ACCAAATACT
GTCCTTCTAG
TGTAGCCGTA
GTTAGGCCAC
CACTTCAAGA
ACTCTGTAGC
ACCGCCTACA
TACCTCGCTC
TGCTAATCCT
GTTACCAGTG
GCTGCTGCCA
GTGGCGATAA
GTCGTGTCTT
ACCGGGTTGG
ACTCAAGACG
ATAGTTACCG
GATAAGGCGC
AGCGGTCGGG
CTGAACGGGG
GGTTCGTGCA
CACAGCCCAG
CTTGGAGCGA
ACGACCTACA
CCGAACTGAG
ATACCTACAG
CGTGAGCTAT
GAGAAAGCGC
CACGCTTCCC
GAAGGGAGAA
AGGCGGACAG
GTATCCGGTA
AGCGGCAGGG
TCGGAACAGG
AGAGCGCACG
AGGGAGCTTC
CAGGGGGAAA
CGCCTGGTAT
CTTTATAGTC
CTGTCGGGTT
TCGCCACCTC
TGACTTGAGC
GTCGATTTTT
GTGATGCTCG
TCAGGGGGGC
GGAGCCTATG
GAAAAACGCC
AGCAACGCGG
CCTTTTTACG
GTTCCTGGCC
TTTTGCTGGC
CTTTTGCTCA
CATGTTCTTT
CCTGCGTTAT
CCCCTGATTC
TGTGGATAAC
CGTATTACCG
CCTTTGAGTG
AGCTGATACC
GCTCGCCGCA
GCCGAACGAC
CGAGCGCAGC
GAGTCAGTGA
GCGAGGAAGC
GGAAGAGCGC
CCAATACGCA
AACCGCCTCT
CCCCGCGCGT
TGGCCGATTC
ATTAATGCAG
CTGTGGAATG
TGTGTCAGTT
AGGGTGTGGA
AAGTCCCCAG
GCTCCCCAGC
AGGCAGAAGT
ATGCAAAGCA
TGCATCTCAA
TTAGTCAGCA
ACCAGGTGTG
GAAAGTCCCC
AGGCTCCCCA
GCAGGCAGAA
GTATGCAAAG
CATGCATCTC
AATTAGTCAG
CAACCATAGT
CCCGCCCCTA
ACTCCGCCCA
TCCCGCCCCT
AACTCCGCCC
AGTTCCGCCC
ATTCTCCGCC
CCATGGCTGA
CTAATTTTTT
TTATTTATGC
AGAGGCCGAG
GCCGCCTCGG
CCTCTGAGCT
ATTCCAGAAG
TAGTGAGGAG
GCTTTTTTGG
AGGCCTAGGC
TTTTGCAAAA
AGCTTGGACA
CAAGACAGGC
TTGCGAGATA
TGTTTGAGAA
TACCACTTTA
TCCCGCGTCA
GGGAGAGGCA
GTGCGTAAAA
AGACGCGGAC
TCATGTGAAA
TACTGGTTTT
TAGTGCGCCA
GATCTCTATA
ATCTCGCGCA
ACCTATTTTC
CCCTCGAACA
CTTTTTAAGC
CGTAGATAAA
CAGGCTGGGA
CACTTCACAT
GAGCGAAAAA
TACATCGTCA
CCTGGGACAT
GTTGCAGATC
CATGCACGTA
AACTCGCAAG
CCGACTGATG
CCTTCTGAAC
AATGGAAAGG
CATTATTGCC
GTAAGCCGTG
GCGGTCTGTA
CCGGGTGCGT
TACTGGCGCG
TGAACTGGGT
ATTCGTCATG
TCGATACCGT
TTGTATTTCC
AGCTACGATC
ACGACAACCA
GCGCGAGCTT
AAAGTGCTGA
AACGCGCAGA
AGGCGATGGC
GAAGGCTTCA
TCGTTATTGA
TGACCTGGTG
GATACCGGTG
GTACTGCGGT
TGCGATTCGT
GAAATGTATC
CAAAAGCGCA
CTTTGTCACC
ATCTTCGCAA
AACCGGCTGG
TCGTCCGCTG
GTTGATGACT
ATGTTGTTGA
TATCCCGCAA
GATACCTGGA
TTGAACAGCC
GTGGGATATG
GGCGTCGTAT
TCGTCCCGCC
AATCTCCGGT
CGCTAATCTT
TTCAACGCCT
GGCACTGCCG
GGCGTTGTTC
TTTTTAACTT
CAGGCGGGTT
ACAATAGTTT
CCAGTAAGTA
TTCTGGAGGC
TGCATCCATG
ACACAGGCAA
ACCTGAGCGA
AACCCTGTTC
AAACCCCGCT
TTAAACATCC
TGAAACCTCG
ACGCTAGTCC
GCCGCTTTAA
TCACGGCGCA
CAACCGCCTG
TGCAGTCGGC
CCTTGATGGT
AAAACCATCC
CTCACTGGTA
TCGCATGATT
AACCGTCTGA
TGTGGATCTG
GCGCGGCATT
GACCCACGCG
AAATCCTCGA
CGTCCAGGCA
CGTATTGTGA
TGAGCGATGC
CGAACGTACC
GACGATGATT
TATACGATAC
GGTGATTGGC
TACCGTGGCG
GCAACTGGAT
TTATGAGTGG
GCCCCGGATC
TTTGTGAAGG
AACCTTACTT
CTGTGGTGTG
ACATAATTGG
ACAAACTACC
TACAGAGATT
TAAAGCTCTA
AGGTAAATAT
AAAATTTTTA
AGTGTATAAT
GTGTTAAACT
ACTGATTCTA
ATTGTTTGTG
TATTTTAGAT
TCCAACCTAT
GGAACTGATG
AATGGGAGCA
GTGGTGGAAT
GCCTTTAATG
AGGAAAACCT
GTTTTGCTCA
GAAGAAATGC
CATCTAGTGA
TGATGAGGCT
ACTGCTGACT
CTCAACATTC
TACTCCTCCA
AAAAAGAAGA
GAAAGGTAGA
AGACCCCAAG
GACTTTCCTT
CAGAATTGCT
AAGTTTTTTG
AGTCATGCTG
TGTTTAGTAA
TAGAACTCTT
GCTTGCTTTG
CTATTTACAC
CACAAAGGAA
AAAGCTGCAC
TGCTATACAA
GAAAATTATG
GAAAAATATT
CTGTAACCTT
TATAAGTAGG
CATAACAGTT
ATAATCATAA
CATACTGTTT
TTTCTTACTC
CACACAGGCA
TAGAGTGTCT
GCTATTAATA
ACTATGCTCA
AAAATTGTGT
ACCTTTAGCT
TTTTAATTTG
TAAAGGGGTT
AATAAGGAAT
ATTTGATGTA
TAGTGCCTTG
ACTAGAGATC
ATAATCAGCC
ATACCACATT
TGTAGAGGTT
TTACTTGCTT
TAAAAAACCT
CCCACACCTC
CCCCTGAACC
TGAAACATAA
AATGAATGCA
ATTGTTGTTG
TTAACTTGTT
TATTGCAGCT
TATAATGGTT
ACAAATAAAG
CAATAGCATC
ACAAATTTCA
CAAATAAAGC
ATTTTTTTCA
CTGCATTCTA
GTTGTGGTTT
GTCCAAACTC
ATCAATGTAT
CTTATCATGT
CTGGATCAAC
TGGATAACTC
AAGCTAACCA
AAATCATCCC
AAACTTCCCA
CCCCATACCC
TATTACCACT
GCCAATTACC
TAGTGGTTTC
ATTTACTCTA
AACCTGTGAT
TCCTCTGAAT
TATTTTCATT
TTAAAGAAAT
TGTATTTGTT
AAATATGTAC
TACAAACTTA
GTAGTTGGAA
GGGCTAATTC
ACTCCCAAAG
AAGACAAGAT
ATCCTTGATC
TGTGGATCTA
CCACACACAA
GGCTACTTCC
CTGATTAGCA
GAACTACACA
CCAGGGCCAG
GGGTCAGATA
TCCACTGACC
TTTGGATGGT
GCTACAAGCT
AGTACCAGTT
GAGCCAGATA
AGGTAGAAGA
GGCCAATAAA
GGAGAGAACA
CCAGCTTGTT
ACACCCTGTG
AGCCTGCATG
GGATGGATGA
CCCGGAGAGA
GAAGTGTTAG
AGTGGAGGTT
TGACAGCCGC
CTAGCATTTC
ATCACGTGGC
CCGAGAGCTG
CATCCGGAGT
ACTTCAAGAA
CTGCTGATAT
CGAGCTTGCT
ACAAGGGACT
TTCCGCTGGG
GACTTTCCAG
GGAGGCGTGG
CCTGGGCGGG
ACTGGGGAGT
GGCGAGCCCT
CAGATCCTGC
ATATAAGCAG
CTGCTTTTTG
CCTGTACTGG
GTCTCTCTGG
TTAGACCAGA
TCTGAGCCTG
GGAGCTCTCT
GGCTAACTAG
GGAACCCACT
GCTTAAGCCT
CAATAAAGCT
TGCCTTGAGT
GCTTCAAGTA
GTGTGTGCCC
GTCTGTTGTG
TGACTCTGGT
AACTAGAGAT
CCCTCAGACC
CTTTTAGTCA
GTGTGGAAAA
TCTCTAGCAG
TGGCGCCCGA
ACAGGGACTT
GAAAGCGAAA
GGGAAACCAG
AGGAGCTCTC
TCGACGCAGG
ACTCGGCTTG
CTGAAGCGCG
CACGGCAAGA
GGCGAGGGGC
GGCGACTGGT
GAGTACGCCA
AAAATTTTGA
CTAGCGGAGG
CTAGAAGGAG
AGAGATGGGT
GCGAGAGCGT
CAGTATTAAG
CGGGGGAGAA
TTAGATCGCG
ATGGGAAAAA
ATTCGGTTAA
GGCCAGGGGG
AAAGAAAAAA
TATAAATTAA
AACATATAGT
ATGGGCAAGC
AGGGAGCTAG
AACGATTCGC
AGTTAATCCT
GGCCTGTTAG
AAACATCAGA
AGGCTGTAGA
CAAATACTGG
GACAGCTACA
ACCATCCCTT
CAGACAGGAT
CAGAAGAACT
TAGATCATTA
TATAATACAG
TAGCAACCCT
CTATTGTGTG
CATCAAAGGA
TAGAGATAAA
AGACACCAAG
GAAGCTTTAG
ACAAGATAGA
GGAAGAGCAA
AACAAAAGTA
AGACCACCGC
ACAGCAAGCG
GCCGGTGATC
TTCAGACCTG
GACGATATAT
ATGAGGGACA
ATTGGAGAAG
TGAATTATAT
AAATATAAAG
TAGTAAAAAT
TGAACCATTA
GGAGTAGCAC
CCACCAAGGC
AAAGAGAAGA
GTGGTGCAGA
GAGAAAAAAG
AGCAGTGGGA
ATAGGAGCTT
TGTTCCTTGG
GTTCTTGGGA
GCAGCAGGAA
GCACTATGGG
CGCAGCGTCA
ATGACGCTGA
CGGTACAGGC
CAGACAATTA
TTGTCTGGTA
TAGTGCAGCA
GCAGAACAAT
TTGCTGAGGG
CTATTGAGGC
GCAACAGCAT
CTGTTGCAAC
TCACAGTCTG
GGGCATCAAG
CAGCTCCAGG
CAAGAATCCT
GGCTGTGGAA
AGATACCTAA
AGGATCAACA
GCTCCTGGGG
ATTTGGGGTT
GCTCTGGAAA
ACTCATTTGC
ACCACTGCTG
TGCCTTGGAA
TGCTAGTTGG
AGTAATAAAT
CTCTGGAACA
GATTTGGAAT
CACACGACCT
GGATGGAGTG
GGACAGAGAA
ATTAACAATT
ACACAAGCTT
AATACACTCC
TTAATTGAAG
AATCGCAAAA
CCAGCAAGAA
AAGAATGAAC
AAGAATTATT
GGAATTAGAT
AAATGGGCAA
GTTTGTGGAA
TTGGTTTAAC
ATAACAAATT
GGCTGTGGTA
TATAAAATTA
TTCATAATGA
TAGTAGGAGG
CTTGGTAGGT
TTAAGAATAG
TTTTTGCTGT
ACTTTCTATA
GTGAATAGAG
TTAGGCAGGG
ATATTCACCA
TTATCGTTTC
AGACCCACCT
CCCAACCCCG
AGGGGACCCG
ACAGGCCCGA
AGGAATAGAA
GAAGAAGGTG
GAGAGAGAGA
CAGAGACAGA
TCCATTCGAT
TAGTGAACGG
ATCTCGACGG
TCG
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