名称: | pLV-Tet-miniCMV-BACH2(human)-GFP-nanobody-mCherry-Puro |
---|---|
别称: | NM_021813.4;BTBD25; IMD60;UTDP4-1 |
基因长: | 2523bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达,四环素诱导 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 红色 |
启动子: | Tet-miniCMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | miniCMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CCAAATGGCA
GTATTCATCC
ACAATTTTAA
AAGAAAAGGG
GGGATTGGGG
GGTACAGTGC
AGGGGAAAGA
ATAGTAGACA
TAATAGCAAC
AGACATACAA
ACTAAAGAAT
TACAAAAACA
AATTACAAAA
ATTCAAAATT
TTCGGGTTTA
TTACAGGGAC
AGCAGAGAGA
ATTCACGTGC
TGCAGGTCCG
AGGTTCTAGA
CGAGTTTACT
CCCTATCAGT
GATAGAGAAC
GATGTCGAGT
TTACTCCCTA
TCAGTGATAG
AGAACGTATG
TCGAGTTTAC
TCCCTATCAG
TGATAGAGAA
CGTATGTCGA
GTTTACTCCC
TATCAGTGAT
AGAGAACGTA
TGTCGAGTTT
ATCCCTATCA
GTGATAGAGA
ACGTATGTCG
AGTTTACTCC
CTATCAGTGA
TAGAGAACGT
ATGTCGAGGT
AGGCGTGTAC
GGTGGGAGGC
CTATATAAGC
AGAGCTCGTT
TAGTGAACCG
TCAGATCGCC
TCTGGCTAAC
TAGAGAACCC
ACTGCTTACT
GGCTTATCGA
AATTAATACG
ACTCACTATA
GGGAGACCCA
AGCTGTCAGA
TCTCGAGCTC
AAGCTTCGAA
TTCTGCGGCG
TACGCAGTGA
ACTCCAAGCC
ACCATGTCTG
TGGATGAGAA
GCCTGACTCC
CCCATGTATG
TGTATGAGTC
CACAGTCCAC
TGCACCAACA
TCCTCCTGGG
CCTCAATGAC
CAGCGGAAAA
AGGATATTCT
CTGTGACGTG
ACTTTGATCG
TGGAGAGGAA
GGAGTTCCGG
GCCCACCGGG
CTGTGCTGGC
CGCATGCAGT
GAATATTTTT
GGCAGGCGCT
GGTTGGACAG
ACAAAAAATG
ATTTGGTGGT
CAGCTTGCCT
GAGGAGGTCA
CAGCCAGGGG
CTTTGGGCCG
CTGTTACAGT
TTGCCTACAC
TGCCAAGCTG
TTACTCAGCA
GAGAAAACAT
CCGCGAGGTC
ATCCGCTGTG
CTGAGTTCCT
GCGCATGCAC
AACCTGGAGG
ACTCCTGCTT
CAGCTTCCTG
CAGACCCAGC
TCCTGAACAG
TGAGGATGGC
CTGTTTGTGT
GCCGGAAGGA
TGCTGCGTGC
CAGCGCCCAC
ACGAGGACTG
CGAGAACTCT
GCAGGAGAGG
AGGAGGATGA
AGAGGAGGAG
ACGATGGATT
CAGAGACGGC
CAAGATGGCT
TGCCCCAGGG
ACCAGATGCT
TCCAGAGCCC
ATCAGCTTTG
AGGCCGCCGC
CATCCCCGTA
GCAGAGAAGG
AAGAAGCCCT
GCTGCCCGAG
CCTGACGTGC
CCACAGACAC
CAAGGAGAGC
TCAGAAAAGG
ACGCGTTAAC
GCAGTACCCC
AGATACAAGA
AATACCAGCT
TGCATGTACC
AAGAATGTCT
ATAATGCATC
ATCACACAGT
ACCTCAGGTT
TTGCAAGCAC
ATTCCGGGAA
GATAACTCTA
GCAACAGCCT
CAAGCCGGGG
CTTGCCAGGG
GGCAGATTAA
AAGTGAGCCG
CCCAGTGAAG
AGAATGAGGA
AGAGAGCATC
ACGCTCTGCC
TGTCTGGAGA
TGAGCCTGAC
GCCAAGGACA
GAGCGGGGGA
TGTCGAGATG
GACCGGAAAC
AGCCCAGCCC
TGCCCCTACC
CCCACGGCCC
CAGCTGGGGC
CGCCTGCCTG
GAGAGATCCA
GGAGCGTGGC
CTCGCCCTCC
TGCTTAAGGT
CTCTGTTCAG
CATAACGAAA
AGTGTGGAGC
TGTCTGGCCT
GCCCAGTACA
TCTCAGCAGC
ACTTTGCCAG
GAGTCCAGCC
TGCCCTTTTG
ACAAGGGGAT
CACTCAGGGT
GACCTTAAAA
CTGACTACAC
CCCTTTCACA
GGGAATTATG
GACAGCCCCA
CGTGGGCCAG
AAGGAGGTGT
CCAACTTCAC
CATGGGGTCG
CCCCTCAGGG
GGCCTGGGTT
GGAGGCTCTC
TGTAAACAGG
AGGGAGAGCT
GGACCGGAGG
AGCGTGATCT
TCTCCTCCAG
CGCTTGTGAC
CAAGTGAGCA
CCTCGGTGCA
TTCTTATTCT
GGGGTGAGCA
GTTTGGACAA
AGACCTCTCT
GAGCCGGTGC
CAAAGGGTCT
GTGGGTGGGA
GCCGGCCAGT
CCCTCCCCAG
CTCGCAGGCC
TACTCCCACG
GTGGGCTGAT
GGCCGACCAC
TTGCCAGGAA
GGATGCGGCC
CAACACCAGC
TGCCCGGTAC
CAATCAAAGT
CTGCCCTCGC
TCACCCCCCT
TGGAGACCAG
GACCAGGACT
TCCAGCTCCT
GCTCTTCCTA
TTCCTACGCG
GAGGACGGGA
GCGGGGGCTC
ACCCTGCAGC
CTCCCTCTCT
GTGAGTTCTC
CTCCTCGCCC
TGTTCCCAGG
GAGCCAGATT
CCTTGCCACA
GAACATCAGG
AACCAGGCCT
GATGGGAGAT
GGAATGTACA
ACCAAGTGCG
GCCCCAAATT
AAATGTGAGC
AGTCTTATGG
AACCAACTCC
AGTGACGAAT
CCGGATCGTT
CTCGGAAGCA
GACAGTGAGT
CGTGTCCTGT
GCAGGACAGG
GGCCAGGAGG
TAAAACTTCC
TTTTCCTGTA
GATCAAATCA
CAGATCTTCC
AAGGAACGAT
TTCCAGATGA
TGATTAAAAT
GCACAAGCTA
ACCTCAGAAC
AGTTAGAGTT
TATTCATGAT
GTCCGACGGC
GCAGCAAGAA
CCGCATCGCG
GCCCAGCGCT
GCCGCAAAAG
GAAACTGGAC
TGTATTCAGA
ATTTAGAATG
TGAAATCCGC
AAATTGGTGT
GTGAGAAAGA
GAAACTGTTG
TCAGAGAGGA
ATCAACTGAA
AGCATGCATG
GGGGAACTGT
TGGACAACTT
CTCCTGCCTT
TCCCAGGAAG
TTTGCCGAGA
CATCCAGAGC
CCCGAGCAGA
TCCAGGCCCT
GCATCGGTAT
TGCCCTGTCC
TCAGACCCAT
GGACTTGCCC
ACGGCCTCCA
GTATTAACCC
TGCGCCCTTG
GGTGCTGAGC
AGAACATTGC
GGCCTCCCAA
TGCGCAGTGG
GGGAAAACGT
GCCCTGCTGC
TTGGAGCCAG
GCGCGGCTCC
CCCCGGACCC
CCCTGGGCAC
CCAGCAACAC
CTCCGAGAAT
TGTACCTCTG
GGAGGAGACT
AGAAGGCACT
GACCCGGGAA
CCTTCTCAGA
GAGAGGACCT
CCTCTTGAAC
CCAGGAGCCA
AACAGTGACC
GTGGACTTCT
GCCAGGAAAT
GACTGATAAG
TGTACAACTG
ACGAACAGCC
CAGGAAAGAT
TATACCTACC
CCTACGACGT
GCCTGACTAC
GCCGGCGGCG
GTGGTTCAGG
CGGTGGCGGT
TCCGGTGGTG
GGGGATCAGT
CCAACTGGTG
GAGTCTGGTG
GCGCTTTGGT
GCAGCCAGGT
GGCTCTCTGC
GTTTGTCCTG
TGCCGCTTCT
GGCTTCCCAG
TGAACCGCTA
TTCCATGCGC
TGGTATCGCC
AGGCTCCAGG
CAAAGAGCGT
GAGTGGGTAG
CCGGTATGTC
CAGCGCGGGT
GATCGTAGCT
CCTATGAAGA
CTCCGTGAAG
GGCCGTTTCA
CCATCAGCCG
TGACGATGCC
CGTAACACGG
TGTATCTGCA
AATGAACAGC
TTGAAACCTG
AAGATACGGC
CGTGTATTAC
TGTAATGTGA
ACGTGGGCTT
CGAGTATTGG
GGCCAAGGCA
CCCAGGTCAC
CGTCTCCAGC
TAAGATCCTT
GACTTGCGGC
CGCAACTCCC
ACCTGCAACA
TGCGTGACTG
ACTGAGGCCG
CGACTCTAGA
GTCGACCTGC
ATCTAGGCGC
CGGAATTAGA
TCTCTCGAGG
TTAACGAATT
CTACCGGGTA
GGGGAGGCGC
TTTTCCCAAG
GCAGTCTGGA
GCATGCGCTT
TAGCAGCCCC
GCTGGGCACT
TGGCGCTACA
CAAGTGGCCT
CTGGCCTCGC
ACACATTCCA
CATCCACCGG
TAGGCGCCAA
CCGGCTCCGT
TCTTTGGTGG
CCCCTTCGCG
CCACCTTCTA
CTCCTCCCCT
AGTCAGGAAG
TTCCCCCCCG
CCCCGCAGCT
CGCGTCGTGC
AGGACGTGAC
AAATGGAAGT
AGCACGTCTC
ACTAGTCTCG
TGCAGATGGA
CAGCACCGCT
GAGCAATGGA
AGCGGGTAGG
CCTTTGGGGC
AGCGGCCAAT
AGCAGCTTTG
CTCCTTCGCT
TTCTGGGCTC
AGAGGCTGGG
AAGGGGTGGG
TCCGGGGGCG
GGCTCAGGGG
CGGGCTCAGG
GGCGGGGCGG
GCGCCCGAAG
GTCCTCCGGA
GGCCCGGCAT
TCTGCACGCT
TCAAAAGCGC
ACGTCTGCCG
CGCTGTTCTC
CTCTTCCTCA
TCTCCGGGCC
TTTCGACCTG
CAGCCCAAGC
TTACCATGGT
GAGCAAGGGC
GAGGAGGATA
ACATGGCCAT
CATCAAGGAG
TTCATGCGCT
TCAAGGTGCA
CATGGAGGGC
TCCGTGAACG
GCCACGAGTT
CGAGATCGAG
GGCGAGGGCG
AGGGCCGCCC
CTACGAGGGC
ACCCAGACCG
CCAAGCTGAA
GGTGACCAAG
GGTGGCCCCC
TGCCCTTCGC
CTGGGACATC
CTGTCCCCTC
AGTTCATGTA
CGGCTCCAAG
GCCTACGTGA
AGCACCCCGC
CGACATCCCC
GACTACTTGA
AGCTGTCCTT
CCCCGAGGGC
TTCAAGTGGG
AGCGCGTGAT
GAACTTCGAG
GACGGCGGCG
TGGTGACCGT
GACCCAGGAC
TCCTCCCTGC
AGGACGGCGA
GTTCATCTAC
AAGGTGAAGC
TGCGCGGCAC
CAACTTCCCC
TCCGACGGCC
CCGTAATGCA
GAAGAAGACC
ATGGGCTGGG
AGGCCTCCTC
CGAGCGGATG
TACCCCGAGG
ACGGCGCCCT
GAAGGGCGAG
ATCAAGCAGA
GGCTGAAGCT
GAAGGACGGC
GGCCACTACG
ACGCTGAGGT
CAAGACCACC
TACAAGGCCA
AGAAGCCCGT
GCAGCTGCCC
GGCGCCTACA
ACGTCAACAT
CAAGTTGGAC
ATCACCTCCC
ACAACGAGGA
CTACACCATC
GTGGAACAGT
ACGAACGCGC
CGAGGGCCGC
CACTCCACCG
GCGGCATGGA
CGAGCTGTAC
AAGGCAACAA
ACTTCTCTCT
GCTGAAACAA
GCCGGAGATG
TCGAAGAGAA
TCCTGGACCG
ATGACCGAGT
ACAAGCCCAC
GGTGCGCCTC
GCCACCCGCG
ACGACGTCCC
CAGGGCCGTA
CGCACCCTCG
CCGCCGCGTT
CGCCGACTAC
CCCGCCACGC
GCCACACCGT
CGATCCGGAC
CGCCACATCG
AGCGGGTCAC
CGAGCTGCAA
GAACTCTTCC
TCACGCGCGT
CGGGCTCGAC
ATCGGCAAGG
TGTGGGTCGC
GGACGACGGC
GCCGCGGTGG
CGGTCTGGAC
CACGCCGGAG
AGCGTCGAAG
CGGGGGCGGT
GTTCGCCGAG
ATCGGCCCGC
GCATGGCCGA
GTTGAGCGGT
TCCCGGCTGG
CCGCGCAGCA
ACAGATGGAA
GGCCTCCTGG
CGCCGCACCG
GCCCAAGGAG
CCCGCGTGGT
TCCTGGCCAC
CGTCGGCGTC
TCGCCCGACC
ACCAGGGCAA
GGGTCTGGGC
AGCGCCGTCG
TGCTCCCCGG
AGTGGAGGCG
GCCGAGCGCG
CCGGGGTGCC
CGCCTTCCTG
GAGACCTCCG
CGCCCCGCAA
CCTCCCCTTC
TACGAGCGGC
TCGGCTTCAC
CGTCACCGCC
GACGTCGAGG
TGCCCGAAGG
ACCGCGCACC
TGGTGCATGA
CCCGCAAGCC
CGGTGCCTGA
TCTTATCGAT
AATCAACCTC
TGGATTACAA
AATTTGTGAA
AGATTGACTG
GTATTCTTAA
CTATGTTGCT
CCTTTTACGC
TATGTGGATA
CGCTGCTTTA
ATGCCTTTGT
ATCATGCTAT
TGCTTCCCGT
ATGGCTTTCA
TTTTCTCCTC
CTTGTATAAA
TCCTGGTTGC
TGTCTCTTTA
TGAGGAGTTG
TGGCCCGTTG
TCAGGCAACG
TGGCGTGGTG
TGCACTGTGT
TTGCTGACGC
AACCCCCACT
GGTTGGGGCA
TTGCCACCAC
CTGTCAGCTC
CTTTCCGGGA
CTTTCGCTTT
CCCCCTCCCT
ATTGCCACGG
CGGAACTCAT
CGCCGCCTGC
CTTGCCCGCT
GCTGGACAGG
GGCTCGGCTG
TTGGGCACTG
ACAATTCCGT
GGTGTTGTCG
GGGAAATCAT
CGTCCTTTCC
TTGGCTGCTC
GCCTGTGTTG
CCACCTGGAT
TCTGCGCGGG
ACGTCCTTCT
GCTACGTCCC
TTCGGCCCTC
AATCCAGCGG
ACCTTCCTTC
CCGCGGCCTG
CTGCCGGCTC
TGCGGCCTCT
TCCGCGTCTT
CGCCTTCGCC
CTCAGACGAG
TCGGATCTCC
CTTTGGGCCG
CCTCCCCGCA
TCGATACCGT
CGACCAAGGC
AGCTGTAGAT
CTTAGCCACT
TTTTAAAAGA
AAAGGGGGGA
CTGGAAGGGC
TAATTCACTC
CCAACGAAGA
CAAGATCTGC
TTTTTGCTTG
TACTGGGTCT
CTCTGGTTAG
ACCAGATCTG
AGCCTGGGAG
CTCTCTGGCT
AACTAGGGAA
CCCACTGCTT
AAGCCTCAAT
AAAGCTTGCC
TTGAGTGCTT
CAAGTAGTGT
GTGCCCGTCT
GTTGTGTGAC
TCTGGTAACT
AGAGATCCCT
CAGACCCTTT
TAGTCAGTGT
GGAAAATCTC
TAGCAGCATC
TAGAATTAAT
TCCGTGTATT
CTATAGTGTC
ACCTAAATCG
TATGTGTATG
ATACATAAGG
TTATGTATTA
ATTGTAGCCG
CGTTCTAACG
ACAATATGTA
CAAGCCTAAT
TGTGTAGCAT
CTGGCTTACT
GAAGCAGACC
CTATCATCTC
TCTCGTAAAC
TGCCGTCAGA
GTCGGTTTGG
TTGGACGAAC
CTTCTGAGTT
TCTGGTAACG
CCGTCCCGCA
CCCGGAAATG
GTCAGCGAAC
CAATCAGCAG
GGTCATCGCT
AGCCAGATCC
TCTACGCCGG
ACGCATCGTG
GCCGGCATCA
CCGGCGCCAC
AGGTGCGGTT
GCTGGCGCCT
ATATCGCCGA
CATCACCGAT
GGGGAAGATC
GGGCTCGCCA
CTTCGGGCTC
ATGAGCGCTT
GTTTCGGCGT
GGGTATGGTG
GCAGGCCCCG
TGGCCGGGGG
ACTGTTGGGC
GCCATCTCCT
TGCATGCACC
ATTCCTTGCG
GCGGCGGTGC
TCAACGGCCT
CAACCTACTA
CTGGGCTGCT
TCCTAATGCA
GGAGTCGCAT
AAGGGAGAGC
GTCGAATGGT
GCACTCTCAG
TACAATCTAG
CTCTGATGCC
GCATAGTTAA
GCCAGCCCCG
ACACCCGCCA
ACACCCGCTG
ACGCGCCCTG
ACGGGCTTGT
CTGCTCCCGG
CATCCGCTTA
CAGACAAGCT
GTGACCGTCT
CCGGGAGCTG
CATGTGTCAG
AGGTTTTCAC
CGTCATCACC
GAAACGCGCG
AGACGAAAGG
GCCTCGTGAT
ACGCCTATTT
TTATAGGTTA
ATGTCATGAT
AATAATGGTT
TCTTAGACGT
CAGGTGGCAC
TTTTCGGGGA
AATGTGCGCG
GAACCCCTAT
TTGTTTATTT
TTCTAAATAC
ATTCAAATAT
GTATCCGCTC
ATGAGACAAT
AACCCTGATA
AATGCTTCAA
TAATATTGAA
AAAGGAAGAG
TATGAGTATT
CAACATTTCC
GTGTCGCCCT
TATTCCCTTT
TTTGCGGCAT
TTTGCCTTCC
TGTTTTTGCT
CACCCAGAAA
CGCTGGTGAA
AGTAAAAGAT
GCTGAAGATC
AGTTGGGTGC
ACGAGTGGGT
TACATCGAAC
TGGATCTCAA
CAGCGGTAAG
ATCCTTGAGA
GTTTTCGCCC
CGAAGAACGT
TTTCCAATGA
TGAGCACTTT
TAAAGTTCTG
CTATGTGGCG
CGGTATTATC
CCGTATTGAC
GCCGGGCAAG
AGCAACTCGG
TCGCCGCATA
CACTATTCTC
AGAATGACTT
GGTTGAGTAC
TCACCAGTCA
CAGAAAAGCA
TCTTACGGAT
GGCATGACAG
TAAGAGAATT
ATGCAGTGCT
GCCATAACCA
TGAGTGATAA
CACTGCGGCC
AACTTACTTC
TGACAACGAT
CGGAGGACCG
AAGGAGCTAA
CCGCTTTTTT
GCACAACATG
GGGGATCATG
TAACTCGCCT
TGATCGTTGG
GAACCGGAGC
TGAATGAAGC
CATACCAAAC
GACGAGCGTG
ACACCACGAT
GCCTGTAGCA
ATGGCAACAA
CGTTGCGCAA
ACTATTAACT
GGCGAACTAC
TTACTCTAGC
TTCCCGGCAA
CAATTAATAG
ACTGGATGGA
GGCGGATAAA
GTTGCAGGAC
CACTTCTGCG
CTCGGCCCTT
CCGGCTGGCT
GGTTTATTGC
TGATAAATCT
GGAGCCGGTG
AGCGTGGGTC
TCGCGGTATC
ATTGCAGCAC
TGGGGCCAGA
TGGTAAGCCC
TCCCGTATCG
TAGTTATCTA
CACGACGGGG
AGTCAGGCAA
CTATGGATGA
ACGAAATAGA
CAGATCGCTG
AGATAGGTGC
CTCACTGATT
AAGCATTGGT
AACTGTCAGA
CCAAGTTTAC
TCATATATAC
TTTAGATTGA
TTTAAAACTT
CATTTTTAAT
TTAAAAGGAT
CTAGGTGAAG
ATCCTTTTTG
ATAATCTCAT
GACCAAAATC
CCTTAACGTG
AGTTTTCGTT
CCACTGAGCG
TCAGACCCCG
TAGAAAAGAT
CAAAGGATCT
TCTTGAGATC
CTTTTTTTCT
GCGCGTAATC
TGCTGCTTGC
AAACAAAAAA
ACCACCGCTA
CCAGCGGTGG
TTTGTTTGCC
GGATCAAGAG
CTACCAACTC
TTTTTCCGAA
GGTAACTGGC
TTCAGCAGAG
CGCAGATACC
AAATACTGTC
CTTCTAGTGT
AGCCGTAGTT
AGGCCACCAC
TTCAAGAACT
CTGTAGCACC
GCCTACATAC
CTCGCTCTGC
TAATCCTGTT
ACCAGTGGCT
GCTGCCAGTG
GCGATAAGTC
GTGTCTTACC
GGGTTGGACT
CAAGACGATA
GTTACCGGAT
AAGGCGCAGC
GGTCGGGCTG
AACGGGGGGT
TCGTGCACAC
AGCCCAGCTT
GGAGCGAACG
ACCTACACCG
AACTGAGATA
CCTACAGCGT
GAGCTATGAG
AAAGCGCCAC
GCTTCCCGAA
GGGAGAAAGG
CGGACAGGTA
TCCGGTAAGC
GGCAGGGTCG
GAACAGGAGA
GCGCACGAGG
GAGCTTCCAG
GGGGAAACGC
CTGGTATCTT
TATAGTCCTG
TCGGGTTTCG
CCACCTCTGA
CTTGAGCGTC
GATTTTTGTG
ATGCTCGTCA
GGGGGGCGGA
GCCTATGGAA
AAACGCCAGC
AACGCGGCCT
TTTTACGGTT
CCTGGCCTTT
TGCTGGCCTT
TTGCTCACAT
GTTCTTTCCT
GCGTTATCCC
CTGATTCTGT
GGATAACCGT
ATTACCGCCT
TTGAGTGAGC
TGATACCGCT
CGCCGCAGCC
GAACGACCGA
GCGCAGCGAG
TCAGTGAGCG
AGGAAGCGGA
AGAGCGCCCA
ATACGCAAAC
CGCCTCTCCC
CGCGCGTTGG
CCGATTCATT
AATGCAGCTG
TGGAATGTGT
GTCAGTTAGG
GTGTGGAAAG
TCCCCAGGCT
CCCCAGCAGG
CAGAAGTATG
CAAAGCATGC
ATCTCAATTA
GTCAGCAACC
AGGTGTGGAA
AGTCCCCAGG
CTCCCCAGCA
GGCAGAAGTA
TGCAAAGCAT
GCATCTCAAT
TAGTCAGCAA
CCATAGTCCC
GCCCCTAACT
CCGCCCATCC
CGCCCCTAAC
TCCGCCCAGT
TCCGCCCATT
CTCCGCCCCA
TGGCTGACTA
ATTTTTTTTA
TTTATGCAGA
GGCCGAGGCC
GCCTCGGCCT
CTGAGCTATT
CCAGAAGTAG
TGAGGAGGCT
TTTTTGGAGG
CCTAGGCTTT
TGCAAAAAGC
TTGGACACAA
GACAGGCTTG
CGAGATATGT
TTGAGAATAC
CACTTTATCC
CGCGTCAGGG
AGAGGCAGTG
CGTAAAAAGA
CGCGGACTCA
TGTGAAATAC
TGGTTTTTAG
TGCGCCAGAT
CTCTATAATC
TCGCGCAACC
TATTTTCCCC
TCGAACACTT
TTTAAGCCGT
AGATAAACAG
GCTGGGACAC
TTCACATGAG
CGAAAAATAC
ATCGTCACCT
GGGACATGTT
GCAGATCCAT
GCACGTAAAC
TCGCAAGCCG
ACTGATGCCT
TCTGAACAAT
GGAAAGGCAT
TATTGCCGTA
AGCCGTGGCG
GTCTGTACCG
GGTGCGTTAC
TGGCGCGTGA
ACTGGGTATT
CGTCATGTCG
ATACCGTTTG
TATTTCCAGC
TACGATCACG
ACAACCAGCG
CGAGCTTAAA
GTGCTGAAAC
GCGCAGAAGG
CGATGGCGAA
GGCTTCATCG
TTATTGATGA
CCTGGTGGAT
ACCGGTGGTA
CTGCGGTTGC
GATTCGTGAA
ATGTATCCAA
AAGCGCACTT
TGTCACCATC
TTCGCAAAAC
CGGCTGGTCG
TCCGCTGGTT
GATGACTATG
TTGTTGATAT
CCCGCAAGAT
ACCTGGATTG
AACAGCCGTG
GGATATGGGC
GTCGTATTCG
TCCCGCCAAT
CTCCGGTCGC
TAATCTTTTC
AACGCCTGGC
ACTGCCGGGC
GTTGTTCTTT
TTAACTTCAG
GCGGGTTACA
ATAGTTTCCA
GTAAGTATTC
TGGAGGCTGC
ATCCATGACA
CAGGCAAACC
TGAGCGAAAC
CCTGTTCAAA
CCCCGCTTTA
AACATCCTGA
AACCTCGACG
CTAGTCCGCC
GCTTTAATCA
CGGCGCACAA
CCGCCTGTGC
AGTCGGCCCT
TGATGGTAAA
ACCATCCCTC
ACTGGTATCG
CATGATTAAC
CGTCTGATGT
GGATCTGGCG
CGGCATTGAC
CCACGCGAAA
TCCTCGACGT
CCAGGCACGT
ATTGTGATGA
GCGATGCCGA
ACGTACCGAC
GATGATTTAT
ACGATACGGT
GATTGGCTAC
CGTGGCGGCA
ACTGGATTTA
TGAGTGGGCC
CCGGATCTTT
GTGAAGGAAC
CTTACTTCTG
TGGTGTGACA
TAATTGGACA
AACTACCTAC
AGAGATTTAA
AGCTCTAAGG
TAAATATAAA
ATTTTTAAGT
GTATAATGTG
TTAAACTACT
GATTCTAATT
GTTTGTGTAT
TTTAGATTCC
AACCTATGGA
ACTGATGAAT
GGGAGCAGTG
GTGGAATGCC
TTTAATGAGG
AAAACCTGTT
TTGCTCAGAA
GAAATGCCAT
CTAGTGATGA
TGAGGCTACT
GCTGACTCTC
AACATTCTAC
TCCTCCAAAA
AAGAAGAGAA
AGGTAGAAGA
CCCCAAGGAC
TTTCCTTCAG
AATTGCTAAG
TTTTTTGAGT
CATGCTGTGT
TTAGTAATAG
AACTCTTGCT
TGCTTTGCTA
TTTACACCAC
AAAGGAAAAA
GCTGCACTGC
TATACAAGAA
AATTATGGAA
AAATATTCTG
TAACCTTTAT
AAGTAGGCAT
AACAGTTATA
ATCATAACAT
ACTGTTTTTT
CTTACTCCAC
ACAGGCATAG
AGTGTCTGCT
ATTAATAACT
ATGCTCAAAA
ATTGTGTACC
TTTAGCTTTT
TAATTTGTAA
AGGGGTTAAT
AAGGAATATT
TGATGTATAG
TGCCTTGACT
AGAGATCATA
ATCAGCCATA
CCACATTTGT
AGAGGTTTTA
CTTGCTTTAA
AAAACCTCCC
ACACCTCCCC
CTGAACCTGA
AACATAAAAT
GAATGCAATT
GTTGTTGTTA
ACTTGTTTAT
TGCAGCTTAT
AATGGTTACA
AATAAAGCAA
TAGCATCACA
AATTTCACAA
ATAAAGCATT
TTTTTCACTG
CATTCTAGTT
GTGGTTTGTC
CAAACTCATC
AATGTATCTT
ATCATGTCTG
GATCAACTGG
ATAACTCAAG
CTAACCAAAA
TCATCCCAAA
CTTCCCACCC
CATACCCTAT
TACCACTGCC
AATTACCTAG
TGGTTTCATT
TACTCTAAAC
CTGTGATTCC
TCTGAATTAT
TTTCATTTTA
AAGAAATTGT
ATTTGTTAAA
TATGTACTAC
AAACTTAGTA
GTTGGAAGGG
CTAATTCACT
CCCAAAGAAG
ACAAGATATC
CTTGATCTGT
GGATCTACCA
CACACAAGGC
TACTTCCCTG
ATTAGCAGAA
CTACACACCA
GGGCCAGGGG
TCAGATATCC
ACTGACCTTT
GGATGGTGCT
ACAAGCTAGT
ACCAGTTGAG
CCAGATAAGG
TAGAAGAGGC
CAATAAAGGA
GAGAACACCA
GCTTGTTACA
CCCTGTGAGC
CTGCATGGGA
TGGATGACCC
GGAGAGAGAA
GTGTTAGAGT
GGAGGTTTGA
CAGCCGCCTA
GCATTTCATC
ACGTGGCCCG
AGAGCTGCAT
CCGGAGTACT
TCAAGAACTG
CTGATATCGA
GCTTGCTACA
AGGGACTTTC
CGCTGGGGAC
TTTCCAGGGA
GGCGTGGCCT
GGGCGGGACT
GGGGAGTGGC
GAGCCCTCAG
ATCCTGCATA
TAAGCAGCTG
CTTTTTGCCT
GTACTGGGTC
TCTCTGGTTA
GACCAGATCT
GAGCCTGGGA
GCTCTCTGGC
TAACTAGGGA
ACCCACTGCT
TAAGCCTCAA
TAAAGCTTGC
CTTGAGTGCT
TCAAGTAGTG
TGTGCCCGTC
TGTTGTGTGA
CTCTGGTAAC
TAGAGATCCC
TCAGACCCTT
TTAGTCAGTG
TGGAAAATCT
CTAGCAGTGG
CGCCCGAACA
GGGACTTGAA
AGCGAAAGGG
AAACCAGAGG
AGCTCTCTCG
ACGCAGGACT
CGGCTTGCTG
AAGCGCGCAC
GGCAAGAGGC
GAGGGGCGGC
GACTGGTGAG
TACGCCAAAA
ATTTTGACTA
GCGGAGGCTA
GAAGGAGAGA
GATGGGTGCG
AGAGCGTCAG
TATTAAGCGG
GGGAGAATTA
GATCGCGATG
GGAAAAAATT
CGGTTAAGGC
CAGGGGGAAA
GAAAAAATAT
AAATTAAAAC
ATATAGTATG
GGCAAGCAGG
GAGCTAGAAC
GATTCGCAGT
TAATCCTGGC
CTGTTAGAAA
CATCAGAAGG
CTGTAGACAA
ATACTGGGAC
AGCTACAACC
ATCCCTTCAG
ACAGGATCAG
AAGAACTTAG
ATCATTATAT
AATACAGTAG
CAACCCTCTA
TTGTGTGCAT
CAAAGGATAG
AGATAAAAGA
CACCAAGGAA
GCTTTAGACA
AGATAGAGGA
AGAGCAAAAC
AAAAGTAAGA
CCACCGCACA
GCAAGCGGCC
GGTGATCTTC
AGACCTGGAC
GATATATATG
AGGGACAATT
GGAGAAGTGA
ATTATATAAA
TATAAAGTAG
TAAAAATTGA
ACCATTAGGA
GTAGCACCCA
CCAAGGCAAA
GAGAAGAGTG
GTGCAGAGAG
AAAAAAGAGC
AGTGGGAATA
GGAGCTTTGT
TCCTTGGGTT
CTTGGGAGCA
GCAGGAAGCA
CTATGGGCGC
AGCGTCAATG
ACGCTGACGG
TACAGGCCAG
ACAATTATTG
TCTGGTATAG
TGCAGCAGCA
GAACAATTTG
CTGAGGGCTA
TTGAGGCGCA
ACAGCATCTG
TTGCAACTCA
CAGTCTGGGG
CATCAAGCAG
CTCCAGGCAA
GAATCCTGGC
TGTGGAAAGA
TACCTAAAGG
ATCAACAGCT
CCTGGGGATT
TGGGGTTGCT
CTGGAAAACT
CATTTGCACC
ACTGCTGTGC
CTTGGAATGC
TAGTTGGAGT
AATAAATCTC
TGGAACAGAT
TTGGAATCAC
ACGACCTGGA
TGGAGTGGGA
CAGAGAAATT
AACAATTACA
CAAGCTTAAT
ACACTCCTTA
ATTGAAGAAT
CGCAAAACCA
GCAAGAAAAG
AATGAACAAG
AATTATTGGA
ATTAGATAAA
TGGGCAAGTT
TGTGGAATTG
GTTTAACATA
ACAAATTGGC
TGTGGTATAT
AAAATTATTC
ATAATGATAG
TAGGAGGCTT
GGTAGGTTTA
AGAATAGTTT
TTGCTGTACT
TTCTATAGTG
AATAGAGTTA
GGCAGGGATA
TTCACCATTA
TCGTTTCAGA
CCCACCTCCC
AACCCCGAGG
GGACCCGACA
GGCCCGAAGG
AATAGAAGAA
GAAGGTGGAG
AGAGAGACAG
AGACAGATCC
ATTCGATTAG
TGAACGGATC
TCGACGGTCG