产品货号：P58179
产品规格：
产品数量：
产品价格：￥0.00
一键复制产品信息
| 名称: | pCMV-AKAP13(human)-TEV-TwinStrep-10×His-Neo |
|---|---|
| 别称: | NM_006738.6AKAP-13; AKAP-Lbc; ARHGEF13; BRX; c-lbc;HA-3; Ht31; LBC; p47; PRKA13; PROTO-LB;PROTO-LBC载体是：P0157 pCDNA3.1(+) BamHI+EcoRV模板是：BC171798（C075-E6）蓝色缺失 |
| 基因长: | 8451bp |
| 质粒宿主： | 哺乳细胞 |
| 质粒用途： | 蛋白表达 |
| 片段类型： | ORF |
| 片段物种： | 人 |
| 原核抗性： | Amp |
| 筛选标记： | Neo/G418 |
| 荧光标记： | |
| 启动子: | CMV |
| 复制子: | pUC |
| 拷贝数: | |
| 感受态: | DH5a |
| 温度: | 37度 |
| 背景载体: | |
| 正向引物: | CMV-F |
| 反向引物: | |
| 诱导方式: |
1、本平台所有产品仅供科研使用，不能用于临床试验，包括人体口服、注射或者体外用药；
2、小包装质粒以模板价格出售，只保证基因部分测序正确，不保证质粒的表达等实验效果；
3、某些质粒即使是出品公司亦没有完整测过序，如关键部位的序列测序正确，亦认为是正确质粒；
4、小包装质粒产品的售后期为您收到产品之日起3个月，收到质粒后请及时转化操作，过期需重新购买；
5、质粒构建皆收取的是成本费用，所有质粒会分享给其他实验者，保密性质粒请提前告知并缴纳保密费用；
6、因科学研究为探索未知的活动，存在较大不确定性，故在任何情况下淼灵都不承担额外的连带责任；
7、发表[中文论文]请标注：产品名称(产品货号) 由武汉淼灵生物科技有限公司提供；
8、发表[英文论文]请标注：Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉（负20度保存，保质期90天，常温运输）
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒；
2、将相应的感受态于冰盒上解冻，取2μl质粒加至100μl感受态中，冰浴 30min；
3、42度热激60s，冰浴2min，加入900μl无抗LB培养基，180rpm震荡培养45min；
4、6000rpm离心3min，留100μl上清重悬，再涂至对应抗性的LB平板上；
5、倒置培养过夜后，如菌落过多则稀释质粒转化，如没有菌落则取10ul质粒转化；
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养，根据需要提取质粒。
二、液体质粒（负20度保存，保质期90天，冰袋运输）
1、收到液体质粒后请甩动保存管，让液体质粒不要吸附在管壁上；
2、根据实验需求取适量质粒，其余保存于负20度冰箱。
三、甘油菌种（负80度保存，保质期90天，冰袋运输）
1、收到甘油菌种后请甩动保存管，让菌液不要吸附在管壁上；
2、用接种环在固体平板上四区划线，如有抗性就加对应抗性；
3、恒温培养箱倒置培养12~16h；
4、务必挑取单菌落接种于液体培养基中，如有抗性就加对应抗性；
5、摇床震荡培养过夜，根据实验需要培养菌种。
GACGGATCGG
GAGATCTCCC
GATCCCCTAT
GGTGCACTCT
CAGTACAATC
TGCTCTGATG
CCGCATAGTT
AAGCCAGTAT
CTGCTCCCTG
CTTGTGTGTT
GGAGGTCGCT
GAGTAGTGCG
CGAGCAAAAT
TTAAGCTACA
ACAAGGCAAG
GCTTGACCGA
CAATTGCATG
AAGAATCTGC
TTAGGGTTAG
GCGTTTTGCG
CTGCTTCGCG
ATGTACGGGC
CAGATATACG
CGTTGACATT
GATTATTGAC
TAGTTATTAA
TAGTAATCAA
TTACGGGGTC
ATTAGTTCAT
AGCCCATATA
TGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCT
CTGGCTAACT
AGAGAACCCA
CTGCTTACTG
GCTTATCGAA
ATTAATACGA
CTCACTATAG
GGAGACCCAA
GCTGGCTAGC
GTTTAAACTT
AAGCTTGGTA
CCGAGCTCGG
ATCCGCCACC
ATGAAACTTA
ATCCACAGCA
AGCTCCCTTA
TATGGTGATT
GTGTTGTTAC
AGTGCTGCTT
GCTGAAGAGG
ACAAAGCTGA
AGATGATGTA
GTGTTTTACT
TGGTATTTTT
GGGTTCCACC
CTCCGTCACT
GTACAAGTAC
TCGGAAGGTC
AGTTCTGATA
CATTGGAGAC
CATTGCTCCT
GGTCATGATT
GTTGTGAAAC
AGTGAAGGTG
CAGCTCTGTG
CTTCCAAAGA
GGGCCTTCCC
GTGTTTGTGG
TGGCTGAAGA
AGACTTTCAT
TTCGTCCAGG
ATGAAGCGTA
TGATGCAGCT
CAATTCCTAG
CAACCAGTGC
TGGAAATCAG
CAGGCTTTGA
ACTTTACCCG
TTTTCTTGAC
CAGTCAGGAC
CCCCATCTGG
GGATGTGAAT
TCCCTTGATA
AGAAGTTGGT
GCTGGCATTC
AGGCACCTGA
AGCTGCCCAC
GGAGTGGAAT
GTATTGGGGA
CAGATCAGAG
TTTGCATGAT
GCTGGCCCGC
GAGAGACATT
GATGCATTTT
GCTGTGCGGC
TGGGACTGCT
GAGGTTGACG
TGGTTCCTGT
TGCAGAAGCC
AGGTGGCCGC
GGAGCTCTCA
GTATCCACAA
CCAGGAAGGG
GCGACGCCTG
TGAGCTTGGC
CTTGGAGCGA
GGCTATCACA
AGCTGCACCA
GCTTCTAACC
GAGGAGAATG
CTGGAGAACC
AGACTCCTGG
AGCAGTTTAT
CCTATGAAAT
ACCGTATGGA
GACTGTTCTG
TGAGGCATCA
TCGAGAGTTG
GACATCTATA
CATTAACCTC
TGAGTCTGAT
TCACATCATG
AACACCCATT
TCCTGGAGAC
GGTTGCACTG
GACCAATTTT
TAAACTTATG
AACATCCAAC
AGCAACTAAT
GAAAACAAAC
CTCAAGCAGA
TGGACAGTCT
TATGCCCTTA
ATGATGACAG
CACAGGATCC
TTCCAGTGCC
CCAGAGACAG
ATGGCCAGTT
TCTTCCCTGT
GCACCGGAGC
CCACGGACCC
TCAGCGACTT
TCTTCTTCTG
AAGAGACTGA
GAGCACTCAG
TGCTGCCCAG
GGAGCCCTGT
TGCACAGACT
GAAAGTCCCT
GTGATTTGTC
AAGCATAGTT
GAGGAGGAGA
ATACAGACCG
TTCCTGTAGG
AAGAAAAATA
AAGGCGTGGA
AAGAAAAGGG
GAAGAGGTGG
AGCCAGCACC
TATTGTGGAC
TCTGGAACTG
TATCTGATCA
AGACAGCTGC
CTTCAGAGCT
TGCCTGATTG
TGGAGTAAAG
GGCACGGAAG
GCCTTTCGTC
CTGTGGAAAC
AGAAATGAAG
AAACTGGAAC
AAAATCTTCT
GGAATGCCCA
CAGACCAGGA
GTCCCTGAGC
AGTGGAGATG
CTGTGCTTCA
GAGAGACTTG
GTCATGGAGC
CAGGCACAGC
CCAGTATTCC
TCTGGAGGTG
AACTGGGAGG
CATTTCAACA
ACAAATGTCA
GTACCCCAGA
CACTGCAGGG
GAAATGGAAC
ATGGGCTCAT
GAACCCAGAT
GCCACTGTTT
GGAAGAATGT
GCTTCAGGGA
GGGGAAAGTA
CAAAGGAAAG
ATTTGAGAAC
TCTAATATTG
GCACAGCTGG
AGCCTCTGAC
GTGCACGTCA
CAAGTAAGCC
TGTGGATAAA
ATCAGTGTTC
CAAACTGTGC
CCCTGCTGCC
AGTTCCCTGG
ATGGTAACAA
ACCTGCTGAG
TCTTCACTTG
CATTTAGTAA
TGAAGAAACC
TCCACTGAAA
AAACAGCAGA
AACGGAAACT
TCACGAAGTC
GTGAGGAGAG
TGCTGATGCT
CCAGTAGATC
AGAATTCTGT
GGTGATTCCA
GCTGCTGCAA
AAGACAAGAT
TTCAGATGGA
TTAGAACCTT
ATACTCTCTT
AGCAGCAGGC
ATAGGTGAGG
CAATGTCACC
CTCAGATTTA
GCCCTTCTTG
GGCTGGAAGA
AGATGTAATG
CCACACCAGA
ACTCAGAAAC
AAATTCATCT
CATGCTCAAA
GCCAAAAGGG
CAAATCCTCA
CCCATTTGTT
CTACAACTGG
AGACGATAAA
CTTTGTGCAG
ACTCTGCATG
TCAACAGAAC
ACAGTGACTT
CTAGTGGCGA
TTTGGTTGCA
AAACTGTGTG
ATAACATAGT
TAGCGAGTCC
GAAAGCACCA
CAGCAAGGCA
ACCCAGCTCA
CAAGATCCAC
CCGATGCCTC
CCACTGTGAA
GACCCACAGG
CTCATACAGT
CACCTCTGAC
CCTGTAAGGG
ATACCCAGGA
ACGTGCGGAT
TTTTGTCCTT
TCAAAGTGGT
GGATAACAAA
GGCCAACGAA
AAGATGTGAA
ACTAGATAAA
CCTTTAACAA
ATATGCTTGA
GGTGGTTTCA
CATCCACATC
CAGTTGTCCC
TAAAATGGAG
AAAGAACTGG
TGCCAGACCA
GGCAGTAATA
TCAGACAGTA
CTTTCTCTCT
GGCAAACAGT
CCAGGCAGTG
AATCAGTAAC
CAAGGATGAC
GCACTTTCTT
TTGTCCCCTC
CCAGAAAGAA
AAGGGAACAG
CAACTCCTGA
ACTACATACA
GCTACAGATT
ATAGAGATGG
CCCAGATGGA
AATTCGAATG
AGCCTGATAC
GCGGCCACTA
GAAGACAGGG
CAGTAGGCCT
GTCCACATCC
TCCACTGCTG
CAGAGCTTCA
GCACGGGATG
GGGAATACCA
GTCTCACAGG
ACTTGGTGGA
GAGCATGAGG
GTCCCGCCCC
TCCAGCAATC
CCAGAAGCTC
TGAATATCAA
GGGGAACACT
GACTCTTCCC
TGCAAAGTGT
GGGTAAGGCC
ACTTTGGCTT
TAGATTCAGT
TTTGACTGAA
GAAGGAAAAC
TTCTGGTGGT
TTCAGAAAGC
TCTGCAGCTC
AGGAACAAGA
TAAGGATAAA
GCGGTGACCT
GTTCCTCTAT
TAAGGAAAAT
GCTCTCTCTT
CAGGAACTTT
GCAGGAAGAG
CAGAGAACAC
CACCTCCTGG
ACAAGATACT
CAACAATTTC
ATGAAAAATC
AATCTCAGCT
GACTGTGCCA
AGGACAAAGC
ACTTCAGCTA
AGTAATTCAC
CGGGTGCATC
CTCTGCCTTT
CTTAAGGCAG
AAACTGAACA
TAACAAGGAA
GTGGCCCCAC
AAGTCTCACT
GCTGACTCAA
GGTGGGGCTG
CCCAGAGCCT
GGTGCCACCA
GGAGCAAGTC
TGGCCACAGA
GTCAAGGCAG
GAAGCCTTGG
GGGCAGAGCA
CAACAGCTCC
GCTCTGTTGC
CATGTCTGTT
GCCAGATGGG
TCTGATGGGT
CCGATGCTCT
TAACTGCAGT
CAGCCTTCTC
CTCTGGATGT
TGGAGTGAAG
AACACTCAAT
CCCAGGGAAA
AACTAGTGCC
TGTGAGGTGA
GTGGAGATGT
GACGGTGGAT
GTTACAGGGG
TTAATGCTCT
ACAAGGTATG
GCTGAGCCCA
GAAGAGAGAA
TATATCACAC
AACACCCAAG
ACATCCTGAT
TCCAAACGTC
TTGTTGAGCC
AAGAGAAGAA
TGCCGTTCTA
GGTTTGCCAG
TGGCTCTACA
GGACAAAGCT
GTGACTGACC
CACAGGGAGT
TGGAACCCCA
GAGATGATAC
CTCTTGATTG
GGAGAAAGGG
AAGCTGGAGG
GAGCAGACCA
CAGCTGTACC
ATGGGTGACG
CTGAGGAAGC
CCAAATAGAC
GATGAAGCAC
ATCCTGTCCT
ACTGCAGCCT
GTTGCCAAGG
AGCTCCCCAC
AGACATGGAG
CTCTCAGCCC
ATGATGATGG
GGCCCCAGCT
GGTGTGAGGG
AAGTCATGCG
AGCCCCGCCT
TCAGGCAGGG
AAAGGAGCAC
TCCCTCTCTA
CCTTGCATGG
TCTCTGCCCA
GGACGCACCT
CTGCCTAAGG
GGGCAGACTT
GATAGAGGAG
GCTGCCAGCC
GTATAGTGGA
TGCTGTCATC
GAACAAGTCA
AGGCCGCTGG
AGCACTGCTT
ACTGAGGGGG
AGGCCTGTCA
CATGTCACTG
TCCAGCCCTG
AGTTGGGTCC
TCTCACTAAA
GGACTAGAGA
GTGCTTTTAC
AGAAAAAGTG
AGTACTTTCC
CACCTGGGGA
GAGCCTACCA
ATGGGCAGTA
CTCCTGAGGA
AGCCACGGGG
AGCCTTGCAG
GATGTTTTGC
TGGAAGGGAG
GAGCCAGAGA
AGATCATTTT
ACCTGTCCAG
GGGCCTGAGC
CAGCAGCAGA
AATGCCAGAC
GTGAAAGCTG
AAGATGAAGT
GGATTTTAGA
GCAAGTTCAA
TTTCTGAAGA
AGTGGCTGTA
GGGAGCATAG
CTGCTACACT
GAAGATGAAG
CAAGGCCCAA
TGACCCAGGC
GATAAACCGA
GAAAACTGGT
GTACAATAGA
GCCATGCCCT
GATGCAGCAT
CTCTTCTGGC
TTCCAAGCAG
AGCCCAGAAT
GTGAGAACTT
CCTGGATGTT
GGACTGGGCA
GAGAGTGTAC
CTCAAAACAA
GGTGTACTTA
AAAGAGAATC
TGGGAGTGAT
TCTGACCTCT
TTCACTCACC
CAGTGATGAC
ATGGACAGCA
TCATCTTCCC
AAAGCCAGAG
GAAGAGCATT
TGGCCTGTGA
TATCACCGGA
TCCAGTTCAT
CCACCGATGA
CACGGCTTCA
CTGGACCGAC
ATTCTTCTCA
TGGCAGTGAT
GTGTCTCTCT
CCCAGATTTT
AAAGCCAAAC
AGGTCAAGAG
ATCGGCAAAG
CCTTGATGGA
TTCTACAGCC
ATGGGATGGG
AGCTGAGGGT
CGAGAAAGTG
AGAGTGAGCC
TGCTGACCCA
GGCGACGTGG
AGGAGGAGGA
GATGGACAGT
ATCACTGAAG
TGCCTGCAAA
CTGCTCTGTC
CTAAGGAGCT
CCATGCGCTC
TCTTTCTCCC
TTCCGGAGGC
ACAGCTGGGG
GCCTGGGAAA
AATGCAGCCA
GCGATGCAGA
AATGAACCAC
CGGAGTATGA
GCTGGTGCCC
CTCTGGTGTG
CAGTACTCTG
CTGGCCTGAG
TGCTGACTTT
AATTACAGAA
GTTTCAGTCT
AGAAGGCTTG
ACAGGAGGAG
CTGGTGTCGG
AAACAAGCCA
TCCTCATCTC
TAGAAGTAAG
CTCTGCAAAT
GCCGAAGAGC
TCAGACACCC
ATTCAGTGGT
GAGGAACGGG
TTGACTCTTT
GGTGTCACTT
TCAGAAGAGG
ATCTGGAGTC
AGACCAGAGA
GAACATAGGA
TGTTTGATCA
GCAGATATGT
CACAGATCTA
AGCAGCAGGG
ATTTAATTAC
TGTACATCAG
CCATTTCCTC
TCCATTGACA
AAATCCATCT
CATTAATGAC
AATCAGCCAT
CCTGGATTGG
ACAATTCACG
GCCCTTCCAC
AGTACCTTCC
ACAATACCAG
TGCTAATCTG
ACTGAGAGTA
TAACAGAAGA
GAACTATAAT
TTCCTGCCAC
ATAGCCCCTC
CAAGAAAGAT
TCTGAATGGA
AGAGTGGAAC
AAAAGTCAGT
CGTACATTCA
GCTACATCAA
GAATAAAATG
TCTAGCAGCA
AGAAGAGCAA
AGAAAAGGAA
AAAGAAAAAG
ATAAGATTAA
GGAGAAGGAG
AAAGATTCTA
AAGACAAGGA
GAAAGATAAG
AAGACTGTCA
ACGGGCACAC
TTTCAGTTCC
ATTCCTGTTG
TGGGTCCCAT
CAGCTGTAGC
CAGTGTATGA
AGCCCTTCAC
CAACAAAGAT
GCCTATACTT
GTGCAAATTG
CAGTGCTTTT
GTCCACAAAG
GCTGCCGAGA
AAGTCTAGCC
TCCTGTGCAA
AGGTCAAAAT
GAAGCAGCCC
AAAGGGAGCC
TTCAGGCACA
TGACACATCA
TCACTGCCCA
CGGTCATTAT
GAGAAACAAG
CCCTCACAGC
CCAAGGAGCG
TCCTCGGTCC
GCAGTCCTCC
TGGTGGATGA
AACCGCTACC
ACCCCAATAT
TTGCCAATAG
ACGATCCCAG
CAGAGTGTCT
CGCTCTCCAA
AAGTGTCTCC
ATACAGAACA
TTACTGGAGT
TGGCAATGAT
GAGAACATGT
CAAACACCTG
GAAATTCCTG
TCTCATTCAA
CAGACTCACT
AAATAAAATC
AGCAAGGTCA
ATGAGTCAAC
AGAATCACTT
ACTGATGAGG
GAGTAGGTAC
AGACATGAAT
GAAGGACAAC
TACTGGGAGA
CTTTGAGATT
GAGTCCAAAC
AGCTGGAAGC
AGAGTCTTGG
AGTCGGATAA
TAGACAGCAA
GTTTCTAAAA
CAGCAAAAGA
AAGATGTGGT
CAAACGGCAA
GAAGTAATAT
ATGAGTTGAT
GCAGACAGAG
TTTCATCATG
TCCGCACTCT
CAAGATCATG
AGTGGTGTGT
ACAGCCAGGG
GATGATGGCG
GATCTGCTTT
TTGAGCAGCA
GATGGTAGAA
AAGCTGTTCC
CCTGTTTGGA
TGAGCTGATC
AGTATCCATA
GCCAATTCTT
CCAGAGGATT
CTGGAGCGGA
AGAAGGAGTC
TCTGGTGGAT
AAAAGTGAAA
AGAACTTTCT
CATCAAGAGG
ATAGGGGATG
TGCTTGTAAA
TCAGTTTTCA
GGTGAGAATG
CAGAACGTTT
AAAGAAGACA
TATGGCAAGT
TTTGTGGGCA
ACATAACCAG
TCTGTAAACT
ACTTCAAAGA
CCTTTATGCC
AAGGATAAGC
GTTTTCAAGC
CTTTGTAAAG
AAGAAGATGA
GCAGTTCAGT
TGTTAGAAGG
CTTGGAATTC
CAGAGTGCAT
ATTGCTTGTA
ACTCAGCGGA
TTACCAAGTA
CCCAGTTTTA
TTCCAAAGAA
TATTGCAGTG
TACCAAAGAC
AATGAAGTGG
AGCAGGAAGA
TCTAGCACAG
TCCTTGAGCC
TGGTGAAGGA
TGTGATTGGA
GCTGTAGACA
GCAAAGTGGC
AAGTTATGAA
AAGAAAGTGC
GTCTCAATGA
GATTTATACA
AAGACAGATA
GCAAGTCAAT
CATGAGGATG
AAGAGTGGTC
AGATGTTTGC
CAAGGAAGAT
TTGAAACGGA
AGAAGCTTGT
ACGTGATGGG
AGTGTGTTTC
TGAAGAATGC
AGCAGGAAGG
TTGAAAGAGG
TTCAAGCAGT
TCTTCTCACT
GACATTTTAG
TTTTCCTTCA
AGAAAAAGAC
CAGAAGTACA
TCTTTGCATC
ATTGGACCAG
AAGTCAACAG
TGATCTCTTT
AAAGAAGCTG
ATTGTGAGAG
AAGTGGCACA
TGAGGAGAAA
GGTTTATTCC
TGATCAGCAT
GGGGATGACA
GATCCAGAGA
TGGTAGAAGT
CCATGCCAGC
TCCAAAGAGG
AACGAAACAG
CTGGATTCAG
ATCATTCAGG
ACACAATCAA
CACCCTGAAC
AGAGATGAAG
ATGAAGGAAT
TCCTAGTGAG
AATGAGGAAG
AAAAGAAAAT
GTTGGACACC
AGAGCCCGAG
AATTAAAAGA
ACAACTTCAC
CAGAAGGACC
AAAAAATCCT
ACTCTTGTTG
GAAGAGAAGG
AGATGATTTT
CCGGGACATG
GCTGAGTGCA
GCACCCCTCT
CCCAGAGGAT
TGCTCCCCAA
CACATAGCCC
TAGAGTTCTC
TTCCGCTCCA
ACACAGAAGA
GGCTCTCAAA
GGAGGACCTT
TAATGAAAAG
TGCAATAAAT
GAGGTGGAGA
TCCTTCAGGG
TTTGGTGAGT
GGAAATCTGG
GAGGCACACT
TGGGCCGACT
GTCAGCAGCC
CCATTGAGCA
AGATGTGGTC
GGTCCCGTTT
CCCTGCCCCG
GAGAGCAGAG
ACCTTTGGAG
GATTTGACAG
CCATCAGATG
AATGCTTCAA
AAGGAGGCGA
GAAGGAAGAG
GGAGATGATG
GCCAAGATCT
TAGGAGAACG
GAATCAGATA
GTGGCCTAAA
AAAGGGTGGA
AATGCTAACC
TGGTATTTAT
GCTTAAAAGA
AACAGTGAGC
AGGTTGTCCA
GAGCGTTGTT
CATCTCTACG
AGCTCCTCAG
CGCTCTGCAG
GGTGTGGTGC
TGCAGCAGGA
CAGCTACATT
GAGGACCAGA
AACTGGTGCT
GAGCGAGAGG
GCGCTCACTC
GCAGCTTGTC
CCGCCCGAGC
TCCCTCATTG
AGCAGGAGAA
GCAGCGCAGC
CTGGAGAAGC
AGCGCCAGGA
CCTGGCCAAC
CTGCAGAAGC
AGCAGGCCCA
GTACCTCGAG
GAGAAGCGCA
GGCGCGAGCG
TGAGTGGGAA
GCTCGTGAGA
GGGAGCTGCG
GGAGCGGGAG
GCCCTCCTGG
CCCAGCGCGA
GGAGGAGGTG
CAGCAGGGGC
AGCAGGACCT
GGAAAAGGAG
CGGGAGGAGC
TCCAGCAGAA
GAAGGGCACA
TACCAGTATG
ACCTGGAGCG
ACTGCGTGCT
GCCCAGAAAC
AGCTTGAGAG
GGAACAGGAG
CAGCTGCGCC
GGGAGGCAGA
GCGGCTCAGC
CAGCGGCAGA
CAGAACGGGA
CCTGTGTCAG
GTTTCCCATC
CACATACCAA
GCTGATGAGG
ATCCCATCGT
TCTTCCCCAG
TCCTGAGGAG
CCCCCCTCGC
CATCTGCACC
TTCCATAGCC
AAATCAGGGT
CATTGGACTC
AGAACTTTCA
GTGTCCCCAA
AAAGGAACAG
CATCTCTCGG
ACACACAAAG
ATAAGGGGCC
TTTTCACATA
CTGAGTTCAA
CCAGCCAGAC
AAACAAAGGA
CCAGAAGGGC
AGAGCCAGGC
CCCTGCGTCC
ACCTCTGCCT
CTACCCGCCT
GTTTGGGTTA
ACAAAGCCAA
AGGAAAAGAA
GGAGAAAAAA
AAGAAGAACA
AAACCAGCCG
CTCTCAGCCC
GGTGATGGTC
CCGCGTCAGA
AGTATCAGCA
GAGGGTGAAG
AGATCTTCTG
CGAATTCGAA
AACCTGTATT
TTCAGGGCTG
GAGCCACCCG
CAGTTCGAGA
AAGGTGGAGG
TTCCGGAGGT
GGATCGGGAG
GTGGATCGTG
GAGCCACCCG
CAGTTCGAAA
AACACCACCA
CCATCACCAT
CATCACCACC
ACTGAGATAT
CCAGCACAGT
GGCGGCCGCT
CGAGTCTAGA
GGGCCCGTTT
AAACCCGCTG
ATCAGCCTCG
ACTGTGCCTT
CTAGTTGCCA
GCCATCTGTT
GTTTGCCCCT
CCCCCGTGCC
TTCCTTGACC
CTGGAAGGTG
CCACTCCCAC
TGTCCTTTCC
TAATAAAATG
AGGAAATTGC
ATCGCATTGT
CTGAGTAGGT
GTCATTCTAT
TCTGGGGGGT
GGGGTGGGGC
AGGACAGCAA
GGGGGAGGAT
TGGGAAGACA
ATAGCAGGCA
TGCTGGGGAT
GCGGTGGGCT
CTATGGCTTC
TGAGGCGGAA
AGAACCAGCT
GGGGCTCTAG
GGGGTATCCC
CACGCGCCCT
GTAGCGGCGC
ATTAAGCGCG
GCGGGTGTGG
TGGTTACGCG
CAGCGTGACC
GCTACACTTG
CCAGCGCCCT
AGCGCCCGCT
CCTTTCGCTT
TCTTCCCTTC
CTTTCTCGCC
ACGTTCGCCG
GCTTTCCCCG
TCAAGCTCTA
AATCGGGGGC
TCCCTTTAGG
GTTCCGATTT
AGTGCTTTAC
GGCACCTCGA
CCCCAAAAAA
CTTGATTAGG
GTGATGGTTC
ACGTAGTGGG
CCATCGCCCT
GATAGACGGT
TTTTCGCCCT
TTGACGTTGG
AGTCCACGTT
CTTTAATAGT
GGACTCTTGT
TCCAAACTGG
AACAACACTC
AACCCTATCT
CGGTCTATTC
TTTTGATTTA
TAAGGGATTT
TGCCGATTTC
GGCCTATTGG
TTAAAAAATG
AGCTGATTTA
ACAAAAATTT
AACGCGAATT
AATTCTGTGG
AATGTGTGTC
AGTTAGGGTG
TGGAAAGTCC
CCAGGCTCCC
CAGCAGGCAG
AAGTATGCAA
AGCATGCATC
TCAATTAGTC
AGCAACCAGG
TGTGGAAAGT
CCCCAGGCTC
CCCAGCAGGC
AGAAGTATGC
AAAGCATGCA
TCTCAATTAG
TCAGCAACCA
TAGTCCCGCC
CCTAACTCCG
CCCATCCCGC
CCCTAACTCC
GCCCAGTTCC
GCCCATTCTC
CGCCCCATGG
CTGACTAATT
TTTTTTATTT
ATGCAGAGGC
CGAGGCCGCC
TCTGCCTCTG
AGCTATTCCA
GAAGTAGTGA
GGAGGCTTTT
TTGGAGGCCT
AGGCTTTTGC
AAAAAGCTCC
CGGGAGCTTG
TATATCCATT
TTCGGATCTG
ATCAAGAGAC
AGGATGAGGA
TCGTTTCGCA
TGATTGAACA
AGATGGATTG
CACGCAGGTT
CTCCGGCCGC
TTGGGTGGAG
AGGCTATTCG
GCTATGACTG
GGCACAACAG
ACAATCGGCT
GCTCTGATGC
CGCCGTGTTC
CGGCTGTCAG
CGCAGGGGCG
CCCGGTTCTT
TTTGTCAAGA
CCGACCTGTC
CGGTGCCCTG
AATGAACTGC
AGGACGAGGC
AGCGCGGCTA
TCGTGGCTGG
CCACGACGGG
CGTTCCTTGC
GCAGCTGTGC
TCGACGTTGT
CACTGAAGCG
GGAAGGGACT
GGCTGCTATT
GGGCGAAGTG
CCGGGGCAGG
ATCTCCTGTC
ATCTCACCTT
GCTCCTGCCG
AGAAAGTATC
CATCATGGCT
GATGCAATGC
GGCGGCTGCA
TACGCTTGAT
CCGGCTACCT
GCCCATTCGA
CCACCAAGCG
AAACATCGCA
TCGAGCGAGC
ACGTACTCGG
ATGGAAGCCG
GTCTTGTCGA
TCAGGATGAT
CTGGACGAAG
AGCATCAGGG
GCTCGCGCCA
GCCGAACTGT
TCGCCAGGCT
CAAGGCGCGC
ATGCCCGACG
GCGAGGATCT
CGTCGTGACC
CATGGCGATG
CCTGCTTGCC
GAATATCATG
GTGGAAAATG
GCCGCTTTTC
TGGATTCATC
GACTGTGGCC
GGCTGGGTGT
GGCGGACCGC
TATCAGGACA
TAGCGTTGGC
TACCCGTGAT
ATTGCTGAAG
AGCTTGGCGG
CGAATGGGCT
GACCGCTTCC
TCGTGCTTTA
CGGTATCGCC
GCTCCCGATT
CGCAGCGCAT
CGCCTTCTAT
CGCCTTCTTG
ACGAGTTCTT
CTGAGCGGGA
CTCTGGGGTT
CGAAATGACC
GACCAAGCGA
CGCCCAACCT
GCCATCACGA
GATTTCGATT
CCACCGCCGC
CTTCTATGAA
AGGTTGGGCT
TCGGAATCGT
TTTCCGGGAC
GCCGGCTGGA
TGATCCTCCA
GCGCGGGGAT
CTCATGCTGG
AGTTCTTCGC
CCACCCCAAC
TTGTTTATTG
CAGCTTATAA
TGGTTACAAA
TAAAGCAATA
GCATCACAAA
TTTCACAAAT
AAAGCATTTT
TTTCACTGCA
TTCTAGTTGT
GGTTTGTCCA
AACTCATCAA
TGTATCTTAT
CATGTCTGTA
TACCGTCGAC
CTCTAGCTAG
AGCTTGGCGT
AATCATGGTC
ATAGCTGTTT
CCTGTGTGAA
ATTGTTATCC
GCTCACAATT
CCACACAACA
TACGAGCCGG
AAGCATAAAG
TGTAAAGCCT
GGGGTGCCTA
ATGAGTGAGC
TAACTCACAT
TAATTGCGTT
GCGCTCACTG
CCCGCTTTCC
AGTCGGGAAA
CCTGTCGTGC
CAGCTGCATT
AATGAATCGG
CCAACGCGCG
GGGAGAGGCG
GTTTGCGTAT
TGGGCGCTCT
TCCGCTTCCT
CGCTCACTGA
CTCGCTGCGC
TCGGTCGTTC
GGCTGCGGCG
AGCGGTATCA
GCTCACTCAA
AGGCGGTAAT
ACGGTTATCC
ACAGAATCAG
GGGATAACGC
AGGAAAGAAC
ATGTGAGCAA
AAGGCCAGCA
AAAGGCCAGG
AACCGTAAAA
AGGCCGCGTT
GCTGGCGTTT
TTCCATAGGC
TCCGCCCCCC
TGACGAGCAT
CACAAAAATC
GACGCTCAAG
TCAGAGGTGG
CGAAACCCGA
CAGGACTATA
AAGATACCAG
GCGTTTCCCC
CTGGAAGCTC
CCTCGTGCGC
TCTCCTGTTC
CGACCCTGCC
GCTTACCGGA
TACCTGTCCG
CCTTTCTCCC
TTCGGGAAGC
GTGGCGCTTT
CTCATAGCTC
ACGCTGTAGG
TATCTCAGTT
CGGTGTAGGT
CGTTCGCTCC
AAGCTGGGCT
GTGTGCACGA
ACCCCCCGTT
CAGCCCGACC
GCTGCGCCTT
ATCCGGTAAC
TATCGTCTTG
AGTCCAACCC
GGTAAGACAC
GACTTATCGC
CACTGGCAGC
AGCCACTGGT
AACAGGATTA
GCAGAGCGAG
GTATGTAGGC
GGTGCTACAG
AGTTCTTGAA
GTGGTGGCCT
AACTACGGCT
ACACTAGAAG
AACAGTATTT
GGTATCTGCG
CTCTGCTGAA
GCCAGTTACC
TTCGGAAAAA
GAGTTGGTAG
CTCTTGATCC
GGCAAACAAA
CCACCGCTGG
TAGCGGTTTT
TTTGTTTGCA
AGCAGCAGAT
TACGCGCAGA
AAAAAAGGAT
CTCAAGAAGA
TCCTTTGATC
TTTTCTACGG
GGTCTGACGC
TCAGTGGAAC
GAAAACTCAC
GTTAAGGGAT
TTTGGTCATG
AGATTATCAA
AAAGGATCTT
CACCTAGATC
CTTTTAAATT
AAAAATGAAG
TTTTAAATCA
ATCTAAAGTA
TATATGAGTA
AACTTGGTCT
GACAGTTACC
AATGCTTAAT
CAGTGAGGCA
CCTATCTCAG
CGATCTGTCT
ATTTCGTTCA
TCCATAGTTG
CCTGACTCCC
CGTCGTGTAG
ATAACTACGA
TACGGGAGGG
CTTACCATCT
GGCCCCAGTG
CTGCAATGAT
ACCGCGAGAC
CCACGCTCAC
CGGCTCCAGA
TTTATCAGCA
ATAAACCAGC
CAGCCGGAAG
GGCCGAGCGC
AGAAGTGGTC
CTGCAACTTT
ATCCGCCTCC
ATCCAGTCTA
TTAATTGTTG
CCGGGAAGCT
AGAGTAAGTA
GTTCGCCAGT
TAATAGTTTG
CGCAACGTTG
TTGCCATTGC
TACAGGCATC
GTGGTGTCAC
GCTCGTCGTT
TGGTATGGCT
TCATTCAGCT
CCGGTTCCCA
ACGATCAAGG
CGAGTTACAT
GATCCCCCAT
GTTGTGCAAA
AAAGCGGTTA
GCTCCTTCGG
TCCTCCGATC
GTTGTCAGAA
GTAAGTTGGC
CGCAGTGTTA
TCACTCATGG
TTATGGCAGC
ACTGCATAAT
TCTCTTACTG
TCATGCCATC
CGTAAGATGC
TTTTCTGTGA
CTGGTGAGTA
CTCAACCAAG
TCATTCTGAG
AATAGTGTAT
GCGGCGACCG
AGTTGCTCTT
GCCCGGCGTC
AATACGGGAT
AATACCGCGC
CACATAGCAG
AACTTTAAAA
GTGCTCATCA
TTGGAAAACG
TTCTTCGGGG
CGAAAACTCT
CAAGGATCTT
ACCGCTGTTG
AGATCCAGTT
CGATGTAACC
CACTCGTGCA
CCCAACTGAT
CTTCAGCATC
TTTTACTTTC
ACCAGCGTTT
CTGGGTGAGC
AAAAACAGGA
AGGCAAAATG
CCGCAAAAAA
GGGAATAAGG
GCGACACGGA
AATGTTGAAT
ACTCATACTC
TTCCTTTTTC
AATATTATTG
AAGCATTTAT
CAGGGTTATT
GTCTCATGAG
CGGATACATA
TTTGAATGTA
TTTAGAAAAA
TAAACAAATA
GGGGTTCCGC
GCACATTTCC
CCGAAAAGTG
CCACCTGACG
TC
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