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pLV3-CMV-KAT5(human)-3×HA-Puro

产品货号:P58132

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pLV3-CMV-KAT5(human)-3×HA-Puro
别称: NM_182710.3;cPLA2; ESA1; HTATIP; HTATIP1; NEDFASB;PLIP; TIP; TIP60; ZC2HC5
基因长: 1638bp
质粒宿主: 哺乳细胞,慢病毒
质粒用途: 蛋白表达
片段类型: ORF
片段物种:
原核抗性: Amp
筛选标记: Puro
荧光标记:
启动子: CMV
复制子: pUC
拷贝数:
感受态: Stbl3
温度: 37度
背景载体:
正向引物: CMV-F
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

ACGCGTGTAG

TCTTATGCAA

TACTCTTGTA

GTCTTGCAAC

ATGGTAACGA

TGAGTTAGCA

61

ACATGCCTTA

CAAGGAGAGA

AAAAGCACCG

TGCATGCCGA

TTGGTGGAAG

TAAGGTGGTA

121

CGATCGTGCC

TTATTAGGAA

GGCAACAGAC

GGGTCTGACA

TGGATTGGAC

GAACCACTGA

181

ATTGCCGCAT

TGCAGAGATA

TTGTATTTAA

GTGCCTAGCT

CGATACAATA

AACGGGTCTC

241

TCTGGTTAGA

CCAGATCTGA

GCCTGGGAGC

TCTCTGGCTA

ACTAGGGAAC

CCACTGCTTA

301

AGCCTCAATA

AAGCTTGCCT

TGAGTGCTTC

AAGTAGTGTG

TGCCCGTCTG

TTGTGTGACT

361

CTGGTAACTA

GAGATCCCTC

AGACCCTTTT

AGTCAGTGTG

GAAAATCTCT

AGCAGTGGCG

421

CCCGAACAGG

GACCTGAAAG

CGAAAGGGAA

ACCAGAGCTC

TCTCGACGCA

GGACTCGGCT

481

TGCTGAAGCG

CGCACGGCAA

GAGGCGAGGG

GCGGCGACTG

GTGAGTACGC

CAAAAATTTT

541

GACTAGCGGA

GGCTAGAAGG

AGAGAGATGG

GTGCGAGAGC

GTCAGTATTA

AGCGGGGGAG

601

AATTAGATCG

CGATGGGAAA

AAATTCGGTT

AAGGCCAGGG

GGAAAGAAAA

AATATAAATT

661

AAAACATATA

GTATGGGCAA

GCAGGGAGCT

AGAACGATTC

GCAGTTAATC

CTGGCCTGTT

721

AGAAACATCA

GAAGGCTGTA

GACAAATACT

GGGACAGCTA

CAACCATCCC

TTCAGACAGG

781

ATCAGAAGAA

CTTAGATCAT

TATATAATAC

AGTAGCAACC

CTCTATTGTG

TGCATCAAAG

841

GATAGAGATA

AAAGACACCA

AGGAAGCTTT

AGACAAGATA

GAGGAAGAGC

AAAACAAAAG

901

TAAGACCACC

GCACAGCAAG

CGGCCACTGA

TCTTCAGACC

TGGAGGAGGA

GATATGAGGG

961

ACAATTGGAG

AAGTGAATTA

TATAAATATA

AAGTAGTAAA

AATTGAACCA

TTAGGAGTAG

1021

CACCCACCAA

GGCAAAGAGA

AGAGTGGTGC

AGAGAGAAAA

AAGAGCAGTG

GGAATAGGAG

1081

CTTTGTTCCT

TGGGTTCTTG

GGAGCAGCAG

GAAGCACTAT

GGGCGCAGCC

TCAATGACGC

1141

TGACGGTACA

GGCCAGACAA

TTATTGTCTG

GTATAGTGCA

GCAGCAGAAC

AATTTGCTGA

1201

GGGCTATTGA

GGCGCAACAG

CATCTGTTGC

AACTCACAGT

CTGGGGCATC

AAGCAGCTCC

1261

AGGCAAGAAT

CCTGGCTGTG

GAAAGATACC

TAAAGGATCA

ACAGCTCCTG

GGGATTTGGG

1321

GTTGCTCTGG

AAAACTCATT

TGCACCACTG

CTGTGCCTTG

GAATGCTAGT

TGGAGTAATA

1381

AATCTCTGGA

ACAGATTGGA

ATCACACGAC

CTGGATGGAG

TGGGACAGAG

AAATTAACAA

1441

TTACACAAGC

TTAATACACT

CCTTAATTGA

AGAATCGCAA

AACCAGCAAG

AAAAGAATGA

1501

ACAAGAATTA

TTGGAATTAG

ATAAATGGGC

AAGTTTGTGG

AATTGGTTTA

ACATAACAAA

1561

TTGGCTGTGG

TATATAAAAT

TATTCATAAT

GATAGTAGGA

GGCTTGGTAG

GTTTAAGAAT

1621

AGTTTTTGCT

GTACTTTCTA

TAGTGAATAG

AGTTAGGCAG

GGATATTCAC

CATTATCGTT

1681

TCAGACCCAC

CTCCCAACCC

CGAGGGGACC

CGACAGGCCC

GAAGGAATAG

AAGAAGAAGG

1741

TGGAGAGAGA

GACAGAGACA

GATCCATTCG

ATTAGTGAAC

GGATCTCGAC

GGTATCGGTT

1801

AACTTTTAAA

AGAAAAGGGG

GGATTGGGGG

GTACAGTGCA

GGGGAAAGAA

TAGTAGACAT

1861

AATAGCAACA

GACATACAAA

CTAAAGAATT

ACAAAAACAA

ATTACAAAAT

TCAAAATTTT

1921

ATCGATACTA

GTATTATGCC

CAGTACATGA

CCTTATGGGA

CTTTCCTACT

TGGCAGTACA

1981

TCTACGTATT

AGTCATCGCT

ATTACCATGG

TGATGCGGTT

TTGGCAGTAC

ATCAATGGGC

2041

GTGGATAGCG

GTTTGACTCA

CGGGGATTTC

CAAGTCTCCA

CCCCATTGAC

GTCAATGGGA

2101

GTTTGTTTTG

GCACCAAAAT

CAACGGGACT

TTCCAAAATG

TCGTAACAAC

TCCGCCCCAT

2161

TGACGCAAAT

GGGCGGTAGG

CGTGTACGGT

GGGAGGTTTA

TATAAGCAGA

GCTCGTTTAG

2221

TGAACCGTCA

GATCGCCTGG

AGACGCCATC

CACGCTGTTT

TGACCTCCAT

AGAAGATTCT

2281

AGAGCTAGCG

AATTCGCCAC

CATGGCGGAG

GTGGTGAGTC

CGGTGCCCGG

GGCGGGGCGG

2341

AGGGAGCCAG

GGGAGGTGGG

TAGAGCCCGA

GGCCCCCCAG

TAGCCGACCC

TGGCGTCGCG

2401

CTGTCTCCCC

AGGGGGAGAT

AATCGAGGGC

TGCCGCCTAC

CCGTGCTGCG

GCGGAACCAG

2461

GACAACGAAG

ATGAGTGGCC

CCTGGCCGAG

ATCCTGAGCG

TGAAGGACAT

CAGTGGCCGG

2521

AAGCTTTTCT

ACGTCCATTA

CATTGACTTC

AACAAACGTC

TGGATGAATG

GGTGACGCAT

2581

GAGCGGCTGG

ACCTAAAGAA

GATCCAGTTC

CCCAAGAAAG

AGGCCAAGAC

CCCCACTAAG

2641

AACGGACTTC

CTGGGTCCCG

TCCTGGCTCT

CCAGAGAGAG

AGGTGCCGGC

CTCGGCGCAG

2701

GCCAGCGGGA

AGACCTTGCC

AATCCCGGTC

CAGATCACAC

TCCGCTTCAA

CCTGCCCAAG

2761

GAGCGGGAGG

CCATTCCCGG

TGGCGAGCCT

GACCAGCCGC

TCTCCTCCAG

CTCCTGCCTG

2821

CAGCCCAACC

ACCGCTCAAC

GAAACGGAAG

GTGGAGGTGG

TTTCACCAGC

AACTCCAGTG

2881

CCCAGCGAGA

CAGCCCCGGC

CTCGGTTTTT

CCCCAGAATG

GAGCCGCCCG

TAGGGCAGTG

2941

GCAGCCCAGC

CAGGACGGAA

GCGAAAATCG

AATTGTTTGG

GCACTGATGA

GGACTCCCAG

3001

GACAGCTCTG

ATGGAATACC

GTCAGCACCA

CGCATGACTG

GCAGCCTGGT

GTCTGATCGA

3061

AGCCACGACG

ACATCGTCAC

CCGGATGAAG

AACATTGAGT

GCATTGAGCT

GGGCCGGCAC

3121

CGCCTCAAGC

CGTGGTACTT

CTCCCCGTAC

CCACAGGAAC

TCACCACATT

GCCTGTCCTC

3181

TACCTGTGCG

AGTTCTGCCT

CAAGTACGGC

CGTAGTCTCA

AGTGTCTTCA

GCGTCATTTG

3241

ACCAAGTGTG

ACCTACGACA

TCCTCCAGGC

AATGAGATTT

ACCGCAAGGG

CACCATCTCC

3301

TTCTTTGAGA

TTGATGGACG

TAAGAACAAG

AGTTATTCCC

AGAACCTGTG

TCTTTTGGCC

3361

AAGTGTTTCC

TTGACCATAA

GACACTGTAC

TATGACACAG

ACCCTTTCCT

CTTCTACGTC

3421

ATGACAGAGT

ATGACTGTAA

GGGCTTCCAC

ATCGTGGGCT

ACTTCTCCAA

GGAGAAAGAA

3481

TCAACGGAAG

ACTACAATGT

GGCCTGCATC

CTAACCCTGC

CTCCCTACCA

GCGCCGGGGC

3541

TACGGCAAGC

TGCTGATCGA

GTTCAGCTAT

GAACTCTCCA

AAGTGGAAGG

GAAAACAGGG

3601

ACCCCTGAGA

AGCCCCTCTC

AGACCTTGGC

CTCCTATCCT

ATCGAAGCTA

CTGGTCCCAG

3661

ACCATCCTGG

AGATCCTGAT

GGGGCTGAAG

TCGGAGAGCG

GGGAGAGGCC

ACAGATCACC

3721

ATCAATGAGA

TTAGTGAAAT

CACCAGCATC

AAGAAGGAGG

ATGTCATCTC

CACTCTGCAG

3781

TACCTCAATC

TCATCAACTA

CTACAAGGGC

CAGTACATCC

TCACACTGTC

AGAGGACATC

3841

GTGGATGGCC

ATGAGCGGGC

CATGCTCAAG

CGGCTCCTGC

GGATCGACTC

CAAGTGTCTG

3901

CACTTCACTC

CCAAGGACTG

GAGCAAGAGG

GGGAAGTGGG

GATCCTACCC

ATACGACGTA

3961

CCTGATTATG

CAACTTATCC

TTATGATGTA

CCTGATTATG

CTAGCTATCC

TTATGATGTC

4021

CCAGACTACG

CATAGGCGGC

CGCGAAGGAT

CTGCGATCGC

TCCGGTGCCC

GTCAGTGGGC

4081

AGAGCGCACA

TCGCCCACAG

TCCCCGAGAA

GTTGGGGGGA

GGGGTCGGCA

ATTGAACGGG

4141

TGCCTAGAGA

AGGTGGCGCG

GGGTAAACTG

GGAAAGTGAT

GTCGTGTACT

GGCTCCGCCT

4201

TTTTCCCGAG

GGTGGGGGAG

AACCGTATAT

AAGTGCAGTA

GTCGCCGTGA

ACGTTCTTTT

4261

TCGCAACGGG

TTTGCCGCCA

GAACACAGCT

GAAGCTTCGA

GGGGCTCGCA

TCTCTCCTTC

4321

ACGCGCCCGC

CGCCCTACCT

GAGGCCGCCA

TCCACGCCGG

TTGAGTCGCG

TTCTGCCGCC

4381

TCCCGCCTGT

GGTGCCTCCT

GAACTGCGTC

CGCCGTCTAG

GTAAGTTTAA

AGCTCAGGTC

4441

GAGACCGGGC

CTTTGTCCGG

CGCTCCCTTG

GAGCCTACCT

AGACTCAGCC

GGCTCTCCAC

4501

GCTTTGCCTG

ACCCTGCTTG

CTCAACTCTA

CGTCTTTGTT

TCGTTTTCTG

TTCTGCGCCG

4561

TTACAGATCC

AAGCTGTGAC

CGGCGCCTAC

GCTAGATGAC

CGAGTACAAG

CCCACGGTGC

4621

GCCTCGCCAC

CCGCGACGAC

GTCCCCCGGG

CCGTACGCAC

CCTCGCCGCC

GCGTTCGCCG

4681

ACTACCCCGC

CACGCGCCAC

ACCGTCGACC

CGGACCGCCA

CATCGAGCGG

GTCACCGAGC

4741

TGCAAGAACT

CTTCCTCACG

CGCGTCGGGC

TCGACATCGG

CAAGGTGTGG

GTCGCGGACG

4801

ACGGCGCCGC

GGTGGCGGTC

TGGACCACGC

CGGAGAGCGT

CGAAGCGGGG

GCGGTGTTCG

4861

CCGAGATCGG

CCCGCGCATG

GCCGAGTTGA

GCGGTTCCCG

GCTGGCCGCG

CAGCAACAGA

4921

TGGAAGGCCT

CCTGGCGCCG

CACCGGCCCA

AGGAGCCCGC

GTGGTTCCTG

GCCACCGTCG

4981

GCGTCTCGCC

CGACCACCAG

GGCAAGGGTC

TGGGCAGCGC

CGTCGTGCTC

CCCGGAGTGG

5041

AGGCGGCCGA

GCGCGCCGGG

GTGCCCGCCT

TCCTGGAGAC

CTCCGCGCCC

CGCAACCTCC

5101

CCTTCTACGA

GCGGCTCGGC

TTCACCGTCA

CCGCCGACGT

CGAGGTGCCC

GAAGGACCGC

5161

GCACCTGGTG

CATGACCCGC

AAGCCCGGTG

CCTGAGTCGA

CAATCAACCT

CTGGATTACA

5221

AAATTTGTGA

AAGATTGACT

GGTATTCTTA

ACTATGTTGC

TCCTTTTACG

CTATGTGGAT

5281

ACGCTGCTTT

AATGCCTTTG

TATCATGCTA

TTGCTTCCCG

TATGGCTTTC

ATTTTCTCCT

5341

CCTTGTATAA

ATCCTGGTTG

CTGTCTCTTT

ATGAGGAGTT

GTGGCCCGTT

GTCAGGCAAC

5401

GTGGCGTGGT

GTGCACTGTG

TTTGCTGACG

CAACCCCCAC

TGGTTGGGGC

ATTGCCACCA

5461

CCTGTCAGCT

CCTTTCCGGG

ACTTTCGCTT

TCCCCCTCCC

TATTGCCACG

GCGGAACTCA

5521

TCGCCGCCTG

CCTTGCCCGC

TGCTGGACAG

GGGCTCGGCT

GTTGGGCACT

GACAATTCCG

5581

TGGTGTTGTC

GGGGAAATCA

TCGTCCTTTC

CTTGGCTGCT

CGCCTGTGTT

GCCACCTGGA

5641

TTCTGCGCGG

GACGTCCTTC

TGCTACGTCC

CTTCGGCCCT

CAATCCAGCG

GACCTTCCTT

5701

CCCGCGGCCT

GCTGCCGGCT

CTGCGGCCTC

TTCCGCGTCT

TCGCCTTCGC

CCTCAGACGA

5761

GTCGGATCTC

CCTTTGGGCC

GCCTCCCCGC

CTGGTACCTT

TAAGACCAAT

GACTTACAAG

5821

GCAGCTGTAG

ATCTTAGCCA

CTTTTTAAAA

GAAAAGGGGG

GACTGGAAGG

GCTAATTCAC

5881

TCCCAACGAA

AATAAGATCT

GCTTTTTGCT

TGTACTGGGT

CTCTCTGGTT

AGACCAGATC

5941

TGAGCCTGGG

AGCTCTCTGG

CTAACTAGGG

AACCCACTGC

TTAAGCCTCA

ATAAAGCTTG

6001

CCTTGAGTGC

TTCAAGTAGT

GTGTGCCCGT

CTGTTGTGTG

ACTCTGGTAA

CTAGAGATCC

6061

CTCAGACCCT

TTTAGTCAGT

GTGGAAAATC

TCTAGCAGTA

GTAGTTCATG

TCATCTTATT

6121

ATTCAGTATT

TATAACTTGC

AAAGAAATGA

ATATCAGAGA

GTGAGAGGAA

CTTGTTTATT

6181

GCAGCTTATA

ATGGTTACAA

ATAAAGCAAT

AGCATCACAA

ATTTCACAAA

TAAAGCATTT

6241

TTTTCACTGC

ATTCTAGTTG

TGGTTTGTCC

AAACTCATCA

ATGTATCTTA

TCATGTCTGG

6301

CTCTAGCTAT

CCCGCCCCTA

ACTCCGCCCA

GTTCCGCCCA

TTCTCCGCCC

CATGGCTGAC

6361

TAATTTTTTT

TATTTATGCA

GAGGCCGAGG

CCGCCTCGGC

CTCTGAGCTA

TTCCAGAAGT

6421

AGTGAGGAGG

CTTTTTTGGA

GGCCTAGACT

TTTGCAGAGA

CGGCCCAAAT

TCGTAATCAT

6481

GGTCATAGCT

GTTTCCTGTG

TGAAATTGTT

ATCCGCTCAC

AATTCCACAC

AACATACGAG

6541

CCGGAAGCAT

AAAGTGTAAA

GCCTGGGGTG

CCTAATGAGT

GAGCTAACTC

ACATTAATTG

6601

CGTTGCGCTC

ACTGCCCGCT

TTCCAGTCGG

GAAACCTGTC

GTGCCAGCTG

CATTAATGAA

6661

TCGGCCAACG

CGCGGGGAGA

GGCGGTTTGC

GTATTGGGCG

CTCTTCCGCT

TCCTCGCTCA

6721

CTGACTCGCT

GCGCTCGGTC

GTTCGGCTGC

GGCGAGCGGT

ATCAGCTCAC

TCAAAGGCGG

6781

TAATACGGTT

ATCCACAGAA

TCAGGGGATA

ACGCAGGAAA

GAACATGTGA

GCAAAAGGCC

6841

AGCAAAAGGC

CAGGAACCGT

AAAAAGGCCG

CGTTGCTGGC

GTTTTTCCAT

AGGCTCCGCC

6901

CCCCTGACGA

GCATCACAAA

AATCGACGCT

CAAGTCAGAG

GTGGCGAAAC

CCGACAGGAC

6961

TATAAAGATA

CCAGGCGTTT

CCCCCTGGAA

GCTCCCTCGT

GCGCTCTCCT

GTTCCGACCC

7021

TGCCGCTTAC

CGGATACCTG

TCCGCCTTTC

TCCCTTCGGG

AAGCGTGGCG

CTTTCTCATA

7081

GCTCACGCTG

TAGGTATCTC

AGTTCGGTGT

AGGTCGTTCG

CTCCAAGCTG

GGCTGTGTGC

7141

ACGAACCCCC

CGTTCAGCCC

GACCGCTGCG

CCTTATCCGG

TAACTATCGT

CTTGAGTCCA

7201

ACCCGGTAAG

ACACGACTTA

TCGCCACTGG

CAGCAGCCAC

TGGTAACAGG

ATTAGCAGAG

7261

CGAGGTATGT

AGGCGGTGCT

ACAGAGTTCT

TGAAGTGGTG

GCCTAACTAC

GGCTACACTA

7321

GAAGGACAGT

ATTTGGTATC

TGCGCTCTGC

TGAAGCCAGT

TACCTTCGGA

AAAAGAGTTG

7381

GTAGCTCTTG

ATCCGGCAAA

CAAACCACCG

CTGGTAGCGG

TGGTTTTTTT

GTTTGCAAGC

7441

AGCAGATTAC

GCGCAGAAAA

AAAGGATCTC

AAGAAGATCC

TTTGATCTTT

TCTACGGGGT

7501

CTGACGCTCA

GTGGAACGAA

AACTCACGTT

AAGGGATTTT

GGTCATGAGA

TTATCAAAAA

7561

GGATCTTCAC

CTAGATCCTT

TTAAATTAAA

AATGAAGTTT

TAAATCAATC

TAAAGTATAT

7621

ATGAGTAAAC

TTGGTCTGAC

AGTTACCAAT

GCTTAATCAG

TGAGGCACCT

ATCTCAGCGA

7681

TCTGTCTATT

TCGTTCATCC

ATAGTTGCCT

GACTCCCCGT

CGTGTAGATA

ACTACGATAC

7741

GGGAGGGCTT

ACCATCTGGC

CCCAGTGCTG

CAATGATACC

GCGAGACCCA

CGCTCACCGG

7801

CTCCAGATTT

ATCAGCAATA

AACCAGCCAG

CCGGAAGGGC

CGAGCGCAGA

AGTGGTCCTG

7861

CAACTTTATC

CGCCTCCATC

CAGTCTATTA

ATTGTTGCCG

GGAAGCTAGA

GTAAGTAGTT

7921

CGCCAGTTAA

TAGTTTGCGC

AACGTTGTTG

CCATTGCTAC

AGGCATCGTG

GTGTCACGCT

7981

CGTCGTTTGG

TATGGCTTCA

TTCAGCTCCG

GTTCCCAACG

ATCAAGGCGA

GTTACATGAT

8041

CCCCCATGTT

GTGCAAAAAA

GCGGTTAGCT

CCTTCGGTCC

TCCGATCGTT

GTCAGAAGTA

8101

AGTTGGCCGC

AGTGTTATCA

CTCATGGTTA

TGGCAGCACT

GCATAATTCT

CTTACTGTCA

8161

TGCCATCCGT

AAGATGCTTT

TCTGTGACTG

GTGAGTACTC

AACCAAGTCA

TTCTGAGAAT

8221

AGTGTATGCG

GCGACCGAGT

TGCTCTTGCC

CGGCGTCAAT

ACGGGATAAT

ACCGCGCCAC

8281

ATAGCAGAAC

TTTAAAAGTG

CTCATCATTG

GAAAACGTTC

TTCGGGGCGA

AAACTCTCAA

8341

GGATCTTACC

GCTGTTGAGA

TCCAGTTCGA

TGTAACCCAC

TCGTGCACCC

AACTGATCTT

8401

CAGCATCTTT

TACTTTCACC

AGCGTTTCTG

GGTGAGCAAA

AACAGGAAGG

CAAAATGCCG

8461

CAAAAAAGGG

AATAAGGGCG

ACACGGAAAT

GTTGAATACT

CATACTCTTC

CTTTTTCAAT

8521

ATTATTGAAG

CATTTATCAG

GGTTATTGTC

TCATGAGCGG

ATACATATTT

GAATGTATTT

8581

AGAAAAATAA

ACAAATAGGG

GTTCCGCGCA

CATTTCCCCG

AAAAGTGCCA

CCTGACGTCT

8641

AAGAAACCAT

TATTATCATG

ACATTAACCT

ATAAAAATAG

GCGTATCACG

AGGCCCTTTC

8701

GTCTCGCGCG

TTTCGGTGAT

GACGGTGAAA

ACCTCTGACA

CATGCAGCTC

CCGGAGACGG

8761

TCACAGCTTG

TCTGTAAGCG

GATGCCGGGA

GCAGACAAGC

CCGTCAGGGC

GCGTCAGCGG

8821

GTGTTGGCGG

GTGTCGGGGC

TGGCTTAACT

ATGCGGCATC

AGAGCAGATT

GTACTGAGAG

8881

TGCACCATAT

GCGGTGTGAA

ATACCGCACA

GATGCGTAAG

GAGAAAATAC

CGCATCAGGC

8941

GCCATTCGCC

ATTCAGGCTG

CGCAACTGTT

GGGAAGGGCG

ATCGGTGCGG

GCCTCTTCGC

9001

TATTACGCCA

GCTGGCGAAA

GGGGGATGTG

CTGCAAGGCG

ATTAAGTTGG

GTAACGCCAG

9061

GGTTTTCCCA

GTCACGACGT

TGTAAAACGA

CGGCCAGTGC

CAAGCTG

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