名称: | pLV3-CMV-Lpl(mouse)-3×FLAG-ZsGreen1-Puro |
---|---|
别称: | NM_008509.2 |
基因长: | 1422bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 小鼠 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | 绿色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ACGCGTGTAG
TCTTATGCAA
TACTCTTGTA
GTCTTGCAAC
ATGGTAACGA
TGAGTTAGCA
ACATGCCTTA
CAAGGAGAGA
AAAAGCACCG
TGCATGCCGA
TTGGTGGAAG
TAAGGTGGTA
CGATCGTGCC
TTATTAGGAA
GGCAACAGAC
GGGTCTGACA
TGGATTGGAC
GAACCACTGA
ATTGCCGCAT
TGCAGAGATA
TTGTATTTAA
GTGCCTAGCT
CGATACAATA
AACGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACCTGAAAG
CGAAAGGGAA
ACCAGAGCTC
TCTCGACGCA
GGACTCGGCT
TGCTGAAGCG
CGCACGGCAA
GAGGCGAGGG
GCGGCGACTG
GTGAGTACGC
CAAAAATTTT
GACTAGCGGA
GGCTAGAAGG
AGAGAGATGG
GTGCGAGAGC
GTCAGTATTA
AGCGGGGGAG
AATTAGATCG
CGATGGGAAA
AAATTCGGTT
AAGGCCAGGG
GGAAAGAAAA
AATATAAATT
AAAACATATA
GTATGGGCAA
GCAGGGAGCT
AGAACGATTC
GCAGTTAATC
CTGGCCTGTT
AGAAACATCA
GAAGGCTGTA
GACAAATACT
GGGACAGCTA
CAACCATCCC
TTCAGACAGG
ATCAGAAGAA
CTTAGATCAT
TATATAATAC
AGTAGCAACC
CTCTATTGTG
TGCATCAAAG
GATAGAGATA
AAAGACACCA
AGGAAGCTTT
AGACAAGATA
GAGGAAGAGC
AAAACAAAAG
TAAGACCACC
GCACAGCAAG
CGGCCACTGA
TCTTCAGACC
TGGAGGAGGA
GATATGAGGG
ACAATTGGAG
AAGTGAATTA
TATAAATATA
AAGTAGTAAA
AATTGAACCA
TTAGGAGTAG
CACCCACCAA
GGCAAAGAGA
AGAGTGGTGC
AGAGAGAAAA
AAGAGCAGTG
GGAATAGGAG
CTTTGTTCCT
TGGGTTCTTG
GGAGCAGCAG
GAAGCACTAT
GGGCGCAGCG
TCAATGACGC
TGACGGTACA
GGCCAGACAA
TTATTGTCTG
GTATAGTGCA
GCAGCAGAAC
AATTTGCTGA
GGGCTATTGA
GGCGCAACAG
CATCTGTTGC
AACTCACAGT
CTGGGGCATC
AAGCAGCTCC
AGGCAAGAAT
CCTGGCTGTG
GAAAGATACC
TAAAGGATCA
ACAGCTCCTG
GGGATTTGGG
GTTGCTCTGG
AAAACTCATT
TGCACCACTG
CTGTGCCTTG
GAATGCTAGT
TGGAGTAATA
AATCTCTGGA
ACAGATTTGG
AATCACACGA
CCTGGATGGA
GTGGGACAGA
GAAATTAACA
ATTACACAAG
CTTAATACAC
TCCTTAATTG
AAGAATCGCA
AAACCAGCAA
GAAAAGAATG
AACAAGAATT
ATTGGAATTA
GATAAATGGG
CAAGTTTGTG
GAATTGGTTT
AACATAACAA
ATTGGCTGTG
GTATATAAAA
TTATTCATAA
TGATAGTAGG
AGGCTTGGTA
GGTTTAAGAA
TAGTTTTTGC
TGTACTTTCT
ATAGTGAATA
GAGTTAGGCA
GGGATATTCA
CCATTATCGT
TTCAGACCCA
CCTCCCAACC
CCGAGGGGAC
CCGACAGGCC
CGAAGGAATA
GAAGAAGAAG
GTGGAGAGAG
AGACAGAGAC
AGATCCATTC
GATTAGTGAA
CGGATCTCGA
CGGTATCGCC
TTTAAAAGAA
AAGGGGGGAT
TGGGGGGTAC
AGTGCAGGGG
AAAGAATAGT
AGACATAATA
GCAACAGACA
TACAAACTAA
AGAATTACAA
AAACAAATTA
CAAAAATTCA
AAATTTTCGG
GTTTATTACA
GGGACAGCAG
AGATCCAGTT
TATCGATAAG
CTTGGGAGTT
CCGCGTTACA
TAACTTACGG
TAAATGGCCC
GCCTGGCTGA
CCGCCCAACG
ACCCCCGCCC
ATTGACGTCA
ATAATGACGT
ATGTTCCCAT
AGTAACGCCA
ATAGGGACTT
TCCATTGACG
TCAATGGGTG
GAGTATTTAC
GGTAAACTGC
CCACTTGGCA
GTACATCAAG
TGTATCATAT
GCCAAGTACG
CCCCCTATTG
ACGTCAATGA
CGGTAAATGG
CCCGCCTGGC
ATTATGCCCA
GTACATGACC
TTATGGGACT
TTCCTACTTG
GCAGTACATC
TACGTATTAG
TCATCGCTAT
TACCATGGTG
ATGCGGTTTT
GGCAGTACAT
CAATGGGCGT
GGATAGCGGT
TTGACTCACG
GGGATTTCCA
AGTCTCCACC
CCATTGACGT
CAATGGGAGT
TTGTTTTGGC
ACCAAAATCA
ACGGGACTTT
CCAAAATGTC
GTAACAACTC
CGCCCCATTG
ACGCAAATGG
GCGGTAGGCG
TGTACGGTGG
GAGGTCTATA
TAAGCAGAGC
TCGTTTAGTG
AACCGTCAGA
TCGCCTGGAG
ACGCCATCCA
CGCTGTTTTG
ACCTCCATAG
AAGACACCGA
CTCTACTAGA
GGATCTATTT
CCGGTGAATT
CGCCACCATG
GAGAGCAAAG
CCCTGCTCCT
GGTGGTCCTG
GGAGTTTGGC
TCCAGAGTTT
GACCGCCTTC
CGAGGAGGGG
TGGCCGCAGC
AGACGCGGGA
AGAGATTTCT
CAGACATCGA
AAGCAAATTT
GCCCTAAGGA
CCCCTGAAGA
CACAGCTGAG
GACACTTGTC
ATCTCATTCC
TGGATTAGCA
GACTCTGTGT
CTAACTGCCA
CTTCAACCAC
AGCAGCAAGA
CCTTCGTGGT
GATCCATGGA
TGGACGGTAA
CGGGAATGTA
TGAGAGTTGG
GTGCCCAAAC
TTGTGGCCGC
CCTGTACAAG
AGAGAACCTG
ACTCCAATGT
CATTGTAGTA
GACTGGTTGT
ATCGGGCCCA
GCAACATTAT
CCAGTGTCAG
CTGGCTACAC
CAAGCTGGTG
GGAAATGATG
TGGCCAGATT
CATCAACTGG
ATGGAGGAGG
AGTTTAACTA
CCCCCTAGAC
AACGTCCACC
TCTTAGGGTA
CAGCCTTGGA
GCCCATGCTG
CTGGCGTAGC
AGGAAGTCTG
ACCAATAAGA
AGGTCAATAG
AATTACTGGT
TTGGATCCAG
CTGGGCCTAA
CTTTGAGTAT
GCAGAAGCCC
CCAGTCGCCT
TTCTCCTGAT
GACGCTGATT
TTGTAGATGT
CTTACACACA
TTTACCAGGG
GGTCACCTGG
TCGAAGTATT
GGAATCCAGA
AACCAGTAGG
GCATGTTGAC
ATTTATCCCA
ATGGAGGCAC
TTTCCAGCCA
GGATGCAACA
TTGGAGAAGC
CATCCGTGTG
ATTGCAGAGA
GAGGACTCGG
AGACGTGGAC
CAGCTGGTGA
AGTGCTCGCA
CGAGCGCTCC
ATTCATCTCT
TCATTGACTC
CCTGCTGAAT
GAAGAAAACC
CCAGCAAGGC
ATACAGGTGC
AACTCCAAGG
AAGCCTTTGA
GAAAGGGCTC
TGCCTGAGTT
GTAGAAAGAA
TCGCTGTAAC
AATCTGGGCT
ATGAGATCAA
CAAGGTCAGA
GCCAAGAGAA
GCAGCAAGAT
GTACCTGAAG
ACTCGCTCTC
AGATGCCCTA
CAAAGTGTTC
CATTACCAAG
TCAAGATTCA
CTTTTCTGGG
ACTGAGGATG
GCAAGCAACA
CAACCAGGCC
TTCGAGATTT
CTCTGTACGG
CACAGTGGCC
GAGAGCGAGA
ACATTCCCTT
CACCCTGCCC
GAGGTTTCCA
CAAATAAAAC
CTACTCCTTC
TTGATTTACA
CGGAGGTGGA
CATCGGAGAA
CTGCTCATGA
TGAAGCTTAA
GTGGATAAGC
GACTCCTACT
TCAGCTGGCC
TGACTGGTGG
AGCAGCCCCA
GCTTCGTCAT
CGAGAGGATC
CGAGTGAAAG
CCGGAGAGAC
TCAGAAAAAG
GTCATCTTCT
GTGCTAGGGA
GAAAGTTTCT
CATCTGCAGA
AGGGAAAGGA
CTCAGCAGTG
TTTGTGAAAT
GCCATGACAA
GTCTCTGAAG
AAGTCTGGCG
GATCCTCGGT
ACCAAGCTTA
AGTGACTACA
AGGATGACGA
TGACAAGGAT
TACAAAGACG
ACGATGATAA
GGACTATAAG
GATGATGACG
ACAAATAAAG
ATCCATCGAT
ACTAGTAAGG
ATCTGCGATC
GCTCCGGTGC
CCGTCAGTGG
GCAGAGCGCA
CATCGCCCAC
AGTCCCCGAG
AAGTTGGGGG
GAGGGGTCGG
CAATTGAACG
GGTGCCTAGA
GAAGGTGGCG
CGGGGTAAAC
TGGGAAAGTG
ATGTCGTGTA
CTGGCTCCGC
CTTTTTCCCG
AGGGTGGGGG
AGAACCGTAT
ATAAGTGCAG
TAGTCGCCGT
GAACGTTCTT
TTTCGCAACG
GGTTTGCCGC
CAGAACACAG
CTGAAGCTTC
GAGGGGCTCG
CATCTCTCCT
TCACGCGCCC
GCCGCCCTAC
CTGAGGCCGC
CATCCACGCC
GGTTGAGTCG
CGTTCTGCCG
CCTCCCGCCT
GTGGTGCCTC
CTGAACTGCG
TCCGCCGTCT
AGGTAAGTTT
AAAGCTCAGG
TCGAGACCGG
GCCTTTGTCC
GGCGCTCCCT
TGGAGCCTAC
CTAGACTCAG
CCGGCTCTCC
ACGCTTTGCC
TGACCCTGCT
TGCTCAACTC
TACGTCTTTG
TTTCGTTTTC
TGTTCTGCGC
CGTTACAGAT
CCAAGCTGTG
ACCGGCGCCT
ACTCTAGCAT
GGCCCAGTCC
AAGCACGGCC
TGACCAAGGA
GATGACCATG
AAGTACCGCA
TGGAGGGCTG
CGTGGACGGC
CACAAGTTCG
TGATCACCGG
CGAGGGCATC
GGCTACCCCT
TCAAGGGCAA
GCAGGCCATC
AACCTGTGCG
TGGTGGAGGG
CGGCCCCTTG
CCCTTCGCCG
AGGACATCTT
GTCCGCCGCC
TTCATGTACG
GCAACCGCGT
GTTCACCGAG
TACCCCCAGG
ACATCGTCGA
CTACTTCAAG
AACTCCTGCC
CCGCCGGCTA
CACCTGGGAC
CGCTCCTTCC
TGTTCGAGGA
CGGCGCCGTG
TGCATCTGCA
ACGCCGACAT
CACCGTGAGC
GTGGAGGAGA
ACTGCATGTA
CCACGAGTCC
AAGTTCTACG
GCGTGAACTT
CCCCGCCGAC
GGCCCCGTGA
TGAAGAAGAT
GACCGACAAC
TGGGAGCCCT
CCTGCGAGAA
GATCATCCCC
GTGCCCAAGC
AGGGCATCTT
GAAGGGCGAC
GTGAGCATGT
ACCTGCTGCT
GAAGGACGGT
GGCCGCTTGC
GCTGCCAGTT
CGACACCGTG
TACAAGGCCA
AGTCCGTGCC
CCGCAAGATG
CCCGACTGGC
ACTTCATCCA
GCACAAGCTG
ACCCGCGAGG
ACCGCAGCGA
CGCCAAGAAC
CAGAAGTGGC
ACCTGACCGA
GCACGCCATC
GCCTCCGGCT
CCGCCTTGCC
CGCGGCCGCT
GAGGGCAGAG
GAAGTCTTCT
AACATGCGGT
GACGTGGAGG
AGAATCCCGG
CCCTATGACC
GAGTACAAGC
CCACGGTGCG
CCTCGCCACC
CGCGACGACG
TCCCCAGGGC
CGTACGCACC
CTCGCCGCCG
CGTTCGCCGA
CTACCCCGCC
ACGCGCCACA
CCGTCGATCC
GGACCGCCAC
ATCGAGCGGG
TCACCGAGCT
GCAAGAACTC
TTCCTCACGC
GCGTCGGGCT
CGACATCGGC
AAGGTGTGGG
TCGCGGACGA
CGGCGCCGCG
GTGGCGGTCT
GGACCACGCC
GGAGAGCGTC
GAAGCGGGGG
CGGTGTTCGC
CGAGATCGGC
CCGCGCATGG
CCGAGTTGAG
CGGTTCCCGG
CTGGCCGCGC
AGCAACAGAT
GGAAGGCCTC
CTGGCGCCGC
ACCGGCCCAA
GGAGCCCGCG
TGGTTCCTGG
CCACCGTCGG
CGTCTCGCCC
GACCACCAGG
GCAAGGGTCT
GGGCAGCGCC
GTCGTGCTCC
CCGGAGTGGA
GGCGGCCGAG
CGCGCCGGGG
TGCCCGCCTT
CCTGGAGACC
TCCGCGCCCC
GCAACCTCCC
CTTCTACGAG
CGGCTCGGCT
TCACCGTCAC
CGCCGACGTC
GAGGTGCCCG
AAGGACCGCG
CACCTGGTGC
ATGACCCGCA
AGCCCGGTGC
CTGAAATCAA
CCTCTGGATT
ACAAAATTTG
TGAAAGATTG
ACTGGTATTC
TTAACTATGT
TGCTCCTTTT
ACGCTATGTG
GATACGCTGC
TTTAATGCCT
TTGTATCATG
CTATTGCTTC
CCGTATGGCT
TTCATTTTCT
CCTCCTTGTA
TAAATCCTGG
TTGCTGTCTC
TTTATGAGGA
GTTGTGGCCC
GTTGTCAGGC
AACGTGGCGT
GGTGTGCACT
GTGTTTGCTG
ACGCAACCCC
CACTGGTTGG
GGCATTGCCA
CCACCTGTCA
GCTCCTTTCC
GGGACTTTCG
CTTTCCCCCT
CCCTATTGCC
ACGGCGGAAC
TCATCGCCGC
CTGCCTTGCC
CGCTGCTGGA
CAGGGGCTCG
GCTGTTGGGC
ACTGACAATT
CCGTGGTGTT
GTCGGGGAAG
CTGACGTCCT
TTCCATGGCT
GCTCGCCTGT
GTTGCCACCT
GGATTCTGCG
CGGGACGTCC
TTCTGCTACG
TCCCTTCGGC
CCTCAATCCA
GCGGACCTTC
CTTCCCGCGG
CCTGCTGCCG
GCTCTGCGGC
CTCTTCCGCG
TCTCCGCCTT
CGCCCTCAGA
CGAGTCGGAT
CTCCCTTTGG
CCGCCTCCCC
GCCTGGTACC
TTTAAGACCA
ATGACTTACA
AGGCAGCTGT
AGATCTTAGC
CACTTTTTAA
AAGAAAAGGG
GGGACTGGAA
GGGCTAATTC
ACTCCCAACG
AAAATAAGAT
CTGCTTTTTG
CTTGTACTGG
GTCTCTCTGG
TTAGACCAGA
TCTGAGCCTG
GGAGCTCTCT
GGCTAACTAG
GGAACCCACT
GCTTAAGCCT
CAATAAAGCT
TGCCTTGAGT
GCTTCAAGTA
GTGTGTGCCC
GTCTGTTGTG
TGACTCTGGT
AACTAGAGAT
CCCTCAGACC
CTTTTAGTCA
GTGTGGAAAA
TCTCTAGCAG
TAGTAGTTCA
TGTCATCTTA
TTATTCAGTA
TTTATAACTT
GCAAAGAAAT
GAATATCAGA
GAGTGAGAGG
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TATCATGTCT
GGCTCTAGCT
ATCCCGCCCC
TAACTCCGCC
CAGTTCCGCC
CATTCTCCGC
CCCATGGCTG
ACTAATTTTT
TTTATTTATG
CAGAGGCCGA
GGCCGCCTCG
GCCTCTGAGC
TATTCCAGAA
GTAGTGAGGA
GGCTTTTTTG
GAGGCCTAGA
CTTTTGCAGA
GACGGCCCAA
ATTCGTAATC
ATGGTCATAG
CTGTTTCCTG
TGTGAAATTG
TTATCCGCTC
ACAATTCCAC
ACAACATACG
AGCCGGAAGC
ATAAAGTGTA
AAGCCTGGGG
TGCCTAATGA
GTGAGCTAAC
TCACATTAAT
TGCGTTGCGC
TCACTGCCCG
CTTTCCAGTC
GGGAAACCTG
TCGTGCCAGC
TGCATTAATG
AATCGGCCAA
CGCGCGGGGA
GAGGCGGTTT
GCGTATTGGG
CGCTCTTCCG
CTTCCTCGCT
CACTGACTCG
CTGCGCTCGG
TCGTTCGGCT
GCGGCGAGCG
GTATCAGCTC
ACTCAAAGGC
GGTAATACGG
TTATCCACAG
AATCAGGGGA
TAACGCAGGA
AAGAACATGT
GAGCAAAAGG
CCAGCAAAAG
GCCAGGAACC
GTAAAAAGGC
CGCGTTGCTG
GCGTTTTTCC
ATAGGCTCCG
CCCCCCTGAC
GAGCATCACA
AAAATCGACG
CTCAAGTCAG
AGGTGGCGAA
ACCCGACAGG
ACTATAAAGA
TACCAGGCGT
TTCCCCCTGG
AAGCTCCCTC
GTGCGCTCTC
CTGTTCCGAC
CCTGCCGCTT
ACCGGATACC
TGTCCGCCTT
TCTCCCTTCG
GGAAGCGTGG
CGCTTTCTCA
TAGCTCACGC
TGTAGGTATC
TCAGTTCGGT
GTAGGTCGTT
CGCTCCAAGC
TGGGCTGTGT
GCACGAACCC
CCCGTTCAGC
CCGACCGCTG
CGCCTTATCC
GGTAACTATC
GTCTTGAGTC
CAACCCGGTA
AGACACGACT
TATCGCCACT
GGCAGCAGCC
ACTGGTAACA
GGATTAGCAG
AGCGAGGTAT
GTAGGCGGTG
CTACAGAGTT
CTTGAAGTGG
TGGCCTAACT
ACGGCTACAC
TAGAAGGACA
GTATTTGGTA
TCTGCGCTCT
GCTGAAGCCA
GTTACCTTCG
GAAAAAGAGT
TGGTAGCTCT
TGATCCGGCA
AACAAACCAC
CGCTGGTAGC
GGTGGTTTTT
TTGTTTGCAA
GCAGCAGATT
ACGCGCAGAA
AAAAAGGATC
TCAAGAAGAT
CCTTTGATCT
TTTCTACGGG
GTCTGACGCT
CAGTGGAACG
AAAACTCACG
TTAAGGGATT
TTGGTCATGA
GATTATCAAA
AAGGATCTTC
ACCTAGATCC
TTTTAAATTA
AAAATGAAGT
TTTAAATCAA
TCTAAAGTAT
ATATGAGTAA
ACTTGGTCTG
ACAGTTACCA
ATGCTTAATC
AGTGAGGCAC
CTATCTCAGC
GATCTGTCTA
TTTCGTTCAT
CCATAGTTGC
CTGACTCCCC
GTCGTGTAGA
TAACTACGAT
ACGGGAGGGC
TTACCATCTG
GCCCCAGTGC
TGCAATGATA
CCGCGAGACC
CACGCTCACC
GGCTCCAGAT
TTATCAGCAA
TAAACCAGCC
AGCCGGAAGG
GCCGAGCGCA
GAAGTGGTCC
TGCAACTTTA
TCCGCCTCCA
TCCAGTCTAT
TAATTGTTGC
CGGGAAGCTA
GAGTAAGTAG
TTCGCCAGTT
AATAGTTTGC
GCAACGTTGT
TGCCATTGCT
ACAGGCATCG
TGGTGTCACG
CTCGTCGTTT
GGTATGGCTT
CATTCAGCTC
CGGTTCCCAA
CGATCAAGGC
GAGTTACATG
ATCCCCCATG
TTGTGCAAAA
AAGCGGTTAG
CTCCTTCGGT
CCTCCGATCG
TTGTCAGAAG
TAAGTTGGCC
GCAGTGTTAT
CACTCATGGT
TATGGCAGCA
CTGCATAATT
CTCTTACTGT
CATGCCATCC
GTAAGATGCT
TTTCTGTGAC
TGGTGAGTAC
TCAACCAAGT
CATTCTGAGA
ATAGTGTATG
CGGCGACCGA
GTTGCTCTTG
CCCGGCGTCA
ATACGGGATA
ATACCGCGCC
ACATAGCAGA
ACTTTAAAAG
TGCTCATCAT
TGGAAAACGT
TCTTCGGGGC
GAAAACTCTC
AAGGATCTTA
CCGCTGTTGA
GATCCAGTTC
GATGTAACCC
ACTCGTGCAC
CCAACTGATC
TTCAGCATCT
TTTACTTTCA
CCAGCGTTTC
TGGGTGAGCA
AAAACAGGAA
GGCAAAATGC
CGCAAAAAAG
GGAATAAGGG
CGACACGGAA
ATGTTGAATA
CTCATACTCT
TCCTTTTTCA
ATATTATTGA
AGCATTTATC
AGGGTTATTG
TCTCATGAGC
GGATACATAT
TTGAATGTAT
TTAGAAAAAT
AAACAAATAG
GGGTTCCGCG
CACATTTCCC
CGAAAAGTGC
CACCTGACGT
CTAAGAAACC
ATTATTATCA
TGACATTAAC
CTATAAAAAT
AGGCGTATCA
CGAGGCCCTT
TCGTCTCGCG
CGTTTCGGTG
ATGACGGTGA
AAACCTCTGA
CACATGCAGC
TCCCGGAGAC
GGTCACAGCT
TGTCTGTAAG
CGGATGCCGG
GAGCAGACAA
GCCCGTCAGG
GCGCGTCAGC
GGGTGTTGGC
GGGTGTCGGG
GCTGGCTTAA
CTATGCGGCA
TCAGAGCAGA
TTGTACTGAG
AGTGCACCAT
ATGCGGTGTG
AAATACCGCA
CAGATGCGTA
AGGAGAAAAT
ACCGCATCAG
GCGCCATTCG
CCATTCAGGC
TGCGCAACTG
TTGGGAAGGG
CGATCGGTGC
GGGCCTCTTC
GCTATTACGC
CAGCTGGCGA
AAGGGGGATG
TGCTGCAAGG
CGATTAAGTT
GGGTAACGCC
AGGGTTTTCC
CAGTCACGAC
GTTGTAAAAC
GACGGCCAGT
GCCAAGCTG