名称: | pLVX-Tight-SSRP1(human) |
---|---|
别称: | NM_003146.3;FACT; FACT80; T160 |
基因长: | 2130bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | TRE |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | Tight-F1(10.31) |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGGAAGGGCT
AATTCACTCC
CAAAGAAGAC
AAGATATCCT
TGATCTGTGG
ATCTACCACA
CACAAGGCTA
CTTCCCTGAT
TAGCAGAACT
ACACACCAGG
GCCAGGGGTC
AGATATCCAC
TGACCTTTGG
ATGGTGCTAC
AAGCTAGTAC
CAGTTGAGCC
AGATAAGGTA
GAAGAGGCCA
ATAAAGGAGA
GAACACCAGC
TTGTTACACC
CTGTGAGCCT
GCATGGGATG
GATGACCCGG
AGAGAGAAGT
GTTAGAGTGG
AGGTTTGACA
GCCGCCTAGC
ATTTCATCAC
GTGGCCCGAG
AGCTGCATCC
GGAGTACTTC
AAGAACTGCT
GATATCGAGC
TTGCTACAAG
GGACTTTCCG
CTGGGGACTT
TCCAGGGAGG
CGTGGCCTGG
GCGGGACTGG
GGAGTGGCGA
GCCCTCAGAT
CCTGCATATA
AGCAGCTGCT
TTTTGCCTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACTTGAAAG
CGAAAGGGAA
ACCAGAGGAG
CTCTCTCGAC
GCAGGACTCG
GCTTGCTGAA
GCGCGCACGG
CAAGAGGCGA
GGGGCGGCGA
CTGGTGAGTA
CGCCAAAAAT
TTTGACTAGC
GGAGGCTAGA
AGGAGAGAGA
TGGGTGCGAG
AGCGTCAGTA
TTAAGCGGGG
GAGAATTAGA
TCGCGATGGG
AAAAAATTCG
GTTAAGGCCA
GGGGGAAAGA
AAAAATATAA
ATTAAAACAT
ATAGTATGGG
CAAGCAGGGA
GCTAGAACGA
TTCGCAGTTA
ATCCTGGCCT
GTTAGAAACA
TCAGAAGGCT
GTAGACAAAT
ACTGGGACAG
CTACAACCAT
CCCTTCAGAC
AGGATCAGAA
GAACTTAGAT
CATTATATAA
TACAGTAGCA
ACCCTCTATT
GTGTGCATCA
AAGGATAGAG
ATAAAAGACA
CCAAGGAAGC
TTTAGACAAG
ATAGAGGAAG
AGCAAAACAA
AAGTAAGACC
ACCGCACAGC
AAGCGGCCGG
CCGCTGATCT
TCAGACCTGG
AGGAGGAGAT
ATGAGGGACA
ATTGGAGAAG
TGAATTATAT
AAATATAAAG
TAGTAAAAAT
TGAACCATTA
GGAGTAGCAC
CCACCAAGGC
AAAGAGAAGA
GTGGTGCAGA
GAGAAAAAAG
AGCAGTGGGA
ATAGGAGCTT
TGTTCCTTGG
GTTCTTGGGA
GCAGCAGGAA
GCACTATGGG
CGCAGCGTCA
ATGACGCTGA
CGGTACAGGC
CAGACAATTA
TTGTCTGGTA
TAGTGCAGCA
GCAGAACAAT
TTGCTGAGGG
CTATTGAGGC
GCAACAGCAT
CTGTTGCAAC
TCACAGTCTG
GGGCATCAAG
CAGCTCCAGG
CAAGAATCCT
GGCTGTGGAA
AGATACCTAA
AGGATCAACA
GCTCCTGGGG
ATTTGGGGTT
GCTCTGGAAA
ACTCATTTGC
ACCACTGCTG
TGCCTTGGAA
TGCTAGTTGG
AGTAATAAAT
CTCTGGAACA
GATTTGGAAT
CACACGACCT
GGATGGAGTG
GGACAGAGAA
ATTAACAATT
ACACAAGCTT
AATACACTCC
TTAATTGAAG
AATCGCAAAA
CCAGCAAGAA
AAGAATGAAC
AAGAATTATT
GGAATTAGAT
AAATGGGCAA
GTTTGTGGAA
TTGGTTTAAC
ATAACAAATT
GGCTGTGGTA
TATAAAATTA
TTCATAATGA
TAGTAGGAGG
CTTGGTAGGT
TTAAGAATAG
TTTTTGCTGT
ACTTTCTATA
GTGAATAGAG
TTAGGCAGGG
ATATTCACCA
TTATCGTTTC
AGACCCACCT
CCCAACCCCG
AGGGGACCCG
ACAGGCCCGA
AGGAATAGAA
GAAGAAGGTG
GAGAGAGAGA
CAGAGACAGA
TCCATTCGAT
TAGTGAACGG
ATCTCGACGG
TATCGCCTTT
AAAAGAAAAG
GGGGGATTGG
GGGGTACAGT
GCAGGGGAAA
GAATAGTAGA
CATAATAGCA
ACAGACATAC
AAACTAAAGA
ACTACAAAAA
CAAATTACAA
AAATTCAAAA
TTTTCGGGTT
TATTACAGGG
ACAGCAGAGA
TCCAGTTTAT
CGATGAGGCC
CTTTCGTCTT
CACTCGAGTT
TACTCCCTAT
CAGTGATAGA
GAACGTATGT
CGAGTTTACT
CCCTATCAGT
GATAGAGAAC
GATGTCGAGT
TTACTCCCTA
TCAGTGATAG
AGAACGTATG
TCGAGTTTAC
TCCCTATCAG
TGATAGAGAA
CGTATGTCGA
GTTTACTCCC
TATCAGTGAT
AGAGAACGTA
TGTCGAGTTT
ATCCCTATCA
GTGATAGAGA
ACGTATGTCG
AGTTTACTCC
CTATCAGTGA
TAGAGAACGT
ATGTCGAGGT
AGGCGTGTAC
GGTGGGAGGC
CTATATAAGC
AGAGCTCGTT
TAGTGAACCG
TCAGATCGCC
TGGAGAAGGA
TCCGCGGCCG
CGCCACCATG
GCAGAGACAC
TGGAGTTCAA
CGACGTCTAT
CAGGAGGTGA
AAGGTTCCAT
GAATGATGGT
CGACTGAGGT
TGAGCCGTCA
GGGCATCATC
TTCAAGAATA
GCAAGACAGG
CAAAGTGGAC
AACATCCAGG
CTGGGGAGTT
AACAGAAGGT
ATCTGGCGCC
GTGTTGCTCT
GGGCCATGGA
CTTAAACTGC
TTACAAAGAA
TGGCCATGTC
TACAAGTATG
ATGGCTTCCG
AGAATCGGAG
TTTGAGAAAC
TCTCTGATTT
CTTCAAAACT
CACTATCGCC
TTGAGCTAAT
GGAGAAGGAC
CTTTGTGTGA
AGGGCTGGAA
CTGGGGGACA
GTGAAATTTG
GTGGGCAGCT
GCTTTCCTTT
GACATTGGTG
ACCAGCCAGT
CTTTGAGATA
CCCCTCAGCA
ATGTGTCCCA
GTGCACCACA
GGCAAGAATG
AGGTGACACT
GGAATTCCAC
CAAAACGATG
ACGCAGAGGT
GTCTCTCATG
GAGGTGCGCT
TCTACGTCCC
ACCCACCCAG
GAGGATGGTG
TGGACCCTGT
TGAGGCCTTT
GCCCAGAATG
TGTTGTCAAA
GGCGGATGTA
ATCCAGGCCA
CGGGAGATGC
CATCTGCATC
TTCCGGGAGC
TGCAGTGTCT
GACTCCTCGT
GGTCGTTATG
ACATTCGGAT
CTACCCCACC
TTTCTGCACC
TGCATGGCAA
GACCTTTGAC
TACAAGATCC
CCTACACCAC
AGTACTGCGT
CTGTTTTTGT
TACCCCACAA
GGACCAGCGC
CAGATGTTCT
TTGTGATCAG
CCTGGATCCC
CCAATCAAGC
AAGGCCAAAC
TCGCTACCAC
TTCCTGATCC
TCCTCTTCTC
CAAGGACGAG
GACATTTCGT
TGACTCTGAA
CATGAACGAG
GAAGAAGTGG
AGAAGCGCTT
TGAGGGTCGG
CTCACCAAGA
ACATGTCAGG
ATCCCTCTAT
GAGATGGTCA
GCCGGGTCAT
GAAAGCACTG
GTAAACCGCA
AGATCACAGT
GCCAGGCAAC
TTCCAAGGGC
ACTCAGGGGC
CCAGTGCATT
ACCTGTTCCT
ACAAGGCAAG
CTCAGGACTG
CTCTACCCGC
TGGAGCGGGG
CTTCATCTAC
GTCCACAAGC
CACCTGTGCA
CATCCGCTTC
GATGAGATCT
CCTTTGTCAA
CTTTGCTCGT
GGTACCACTA
CTACTCGTTC
CTTTGACTTT
GAAATTGAGA
CCAAGCAGGG
CACTCAGTAT
ACCTTCAGCA
GCATTGAGAG
GGAGGAGTAC
GGGAAACTGT
TTGATTTTGT
CAACGCGAAA
AAGCTCAACA
TCAAAAACCG
AGGATTGAAA
GAGGGCATGA
ACCCAAGCTA
CGATGAATAT
GCTGACTCTG
ATGAGGACCA
GCATGATGCC
TACTTGGAGA
GGATGAAGGA
GGAAGGCAAG
ATCCGGGAGG
AGAATGCCAA
TGACAGCAGC
GATGACTCAG
GAGAAGAAAC
CGATGAGTCA
TTCAACCCAG
GTGAAGAGGA
GGAAGATGTG
GCAGAGGAGT
TTGACAGCAA
CGCCTCTGCC
AGCTCCTCCA
GTAATGAGGG
TGACAGTGAC
CGGGATGAGA
AGAAGCGGAA
ACAGCTCAAA
AAGGCCAAGA
TGGCCAAGGA
CCGCAAGAGC
CGCAAGAAGC
CTGTGGAGGT
GAAGAAGGGC
AAAGACCCCA
ATGCCCCCAA
GAGGCCCATG
TCTGCATACA
TGCTGTGGCT
CAATGCCAGC
CGAGAGAAGA
TCAAGTCAGA
CCATCCTGGC
ATCAGCATCA
CGGATCTTTC
CAAGAAGGCA
GGCGAGATCT
GGAAGGGAAT
GTCCAAAGAG
AAGAAAGAGG
AGTGGGATCG
CAAGGCTGAG
GATGCCAGGA
GGGACTATGA
AAAAGCCATG
AAAGAATATG
AAGGGGGCCG
AGGCGAGTCT
TCTAAGAGGG
ACAAGTCAAA
GAAGAAGAAG
AAAGTAAAGG
TAAAGATGGA
AAAGAAATCC
ACGCCCTCTA
GGGGCTCATC
ATCCAAGTCG
TCCTCAAGGC
AGCTAAGCGA
GAGCTTCAAG
AGCAAAGAGT
TTGTGTCTAG
TGATGAGAGC
TCTTCGGGAG
AGAACAAGAG
CAAAAAGAAG
AGGAGGAGGA
GCGAGGACTC
TGAAGAAGAA
GAACTAGCCA
GTACTCCCCC
CAGCTCAGAG
GACTCAGCGT
CAGGATCCGA
TGAGTAGACG
CGTGAATTCC
AACCTCTGGA
TTACAAAATT
TGTGAAAGAT
TGACTGGTAT
TCTTAACTAT
GTTGCTCCTT
TTACGCTATG
TGGATACGCT
GCTTTAATGC
CTTTGTATCA
TGCTATTGCT
TCCCGTATGG
CTTTCATTTT
CTCCTCCTTG
TATAAATCCT
GGTTGCTGTC
TCTTTATGAG
GAGTTGTGGC
CCGTTGTCAG
GCAACGTGGC
GTGGTGTGCA
CTGTGTTTGC
TGACGCAACC
CCCACTGGTT
GGGGCATTGC
CACCACCTGT
CAGCTCCTTT
CCGGGACTTT
CGCTTTCCCC
CTCCCTATTG
CCACGGCGGA
ACTCATCGCC
GCCTGCCTTG
CCCGCTGCTG
GACAGGGGCT
CGGCTGTTGG
GCACTGACAA
TTCCGTGGTG
TTGTCGGGGA
AGCTGACGTC
CTTTCCATGG
CTGCTCGCCT
GTGTTGCCAC
CTGGATTCTG
CGCGGGACGT
CCTTCTGCTA
CGTCCCTTCG
GCCCTCAATC
CAGCGGACCT
TCCTTCCCGC
GGCCTGCTGC
CGGCTCTGCG
GCCTCTTCCG
CGTCTTCGCC
TTCGCCCTCA
GACGAGTCGG
ATCTCCCTTT
GGGCCGCCTC
CCCGCCTGGA
AGTTTAAACC
TGCAGTCGAG
ACCTAGAAAA
ACATGGAGCA
ATCACAAGTA
GCAATACAGC
AGCTACCAAT
GCTGATTGTG
CCTGGCTAGA
AGCACAAGAG
GAGGAGGAGG
TGGGTTTTTC
CAGTCACACC
TCAGGTACCT
TTAAGACCAA
TGACTTACAA
GGCAGCTGTA
GATCTTAGCC
ACTTTTTAAA
AGAAAAGAGG
GGACTGCTGG
AAGGGCTAAT
TCACTCCCAA
CGAAGACAAG
ATATCCTTGA
TCTGTGGATC
TACCACACAC
AAGGCTACTT
CCCTGATTAG
CAGAACTACA
CACCAGGGCC
AGGGGTCAGA
TATCCACTGA
CCTTTGGATG
GTGCTACAAG
CTAGTACCAG
TTGAGCCAGA
TAAGGTAGAA
GAGGCCAATA
AAGGAGAGAA
CACCAGCTTG
TTACACCCTG
TGAGCCTGCA
TGGGATGGAT
GACCCGGAGA
GAGAAGTGTT
AGAGTGGAGG
TTTGACAGCC
GCCTAGCATT
TCATCACGTG
GCCCGAGAGC
TGCATCCGGA
GTACTTCAAG
AACTGCTGAT
ATCGAGCTTG
CTACAAGGGA
CTTTCCGCTG
GGGACTTTCC
AGGGAGGCGT
GGCCTGGGCG
GGACTGGGGA
GTGGCGAGCC
CTCAGATCCT
GCATATAAGC
AGCTGCTTTT
TGCCTGTACT
GGGTCTCTCT
GGTTAGACCA
GATCTGAGCC
TGGGAGCTCT
CTGGCTAACT
AGGGAACCCA
CTGCTTAAGC
CTCAATAAAG
CTTGCCTTGA
GTGCTTCAAG
TAGTGTGTGC
CCGTCTGTTG
TGTGACTCTG
GTAACTAGAG
ATCCCTCAGA
CCCTTTTAGT
CAGTGTGGAA
AATCTCTAGC
AGTAGTAGTT
CATGTCATCT
TATTATTCAG
TATTTATAAC
TTGCAAAGAA
ATGAATATCA
GAGAGTGAGA
GGCCTTGACA
TTGCTAGCGT
TTTACCGTCG
ACCTCTAGCT
AGAGCTTGGC
GTAATCATGG
TCATAGCTGT
TTCCTGTGTG
AAATTGTTAT
CCGCTCACAA
TTCCACACAA
CATACGAGCC
GGAAGCATAA
AGTGTAAAGC
CTGGGGTGCC
TAATGAGTGA
GCTAACTCAC
ATTAATTGCG
TTGCGCTCAC
TGCCCGCTTT
CCAGTCGGGA
AACCTGTCGT
GCCAGCTGCA
TTAATGAATC
GGCCAACGCG
CGGGGAGAGG
CGGTTTGCGT
ATTGGGCGCT
CTTCCGCTTC
CTCGCTCACT
GACTCGCTGC
GCTCGGTCGT
TCGGCTGCGG
CGAGCGGTAT
CAGCTCACTC
AAAGGCGGTA
ATACGGTTAT
CCACAGAATC
AGGGGATAAC
GCAGGAAAGA
ACATGTGAGC
AAAAGGCCAG
CAAAAGGCCA
GGAACCGTAA
AAAGGCCGCG
TTGCTGGCGT
TTTTCCATAG
GCTCCGCCCC
CCTGACGAGC
ATCACAAAAA
TCGACGCTCA
AGTCAGAGGT
GGCGAAACCC
GACAGGACTA
TAAAGATACC
AGGCGTTTCC
CCCTGGAAGC
TCCCTCGTGC
GCTCTCCTGT
TCCGACCCTG
CCGCTTACCG
GATACCTGTC
CGCCTTTCTC
CCTTCGGGAA
GCGTGGCGCT
TTCTCATAGC
TCACGCTGTA
GGTATCTCAG
TTCGGTGTAG
GTCGTTCGCT
CCAAGCTGGG
CTGTGTGCAC
GAACCCCCCG
TTCAGCCCGA
CCGCTGCGCC
TTATCCGGTA
ACTATCGTCT
TGAGTCCAAC
CCGGTAAGAC
ACGACTTATC
GCCACTGGCA
GCAGCCACTG
GTAACAGGAT
TAGCAGAGCG
AGGTATGTAG
GCGGTGCTAC
AGAGTTCTTG
AAGTGGTGGC
CTAACTACGG
CTACACTAGA
AGAACAGTAT
TTGGTATCTG
CGCTCTGCTG
AAGCCAGTTA
CCTTCGGAAA
AAGAGTTGGT
AGCTCTTGAT
CCGGCAAACA
AACCACCGCT
GGTAGCGGTT
TTTTTGTTTG
CAAGCAGCAG
ATTACGCGCA
GAAAAAAAGG
ATCTCAAGAA
GATCCTTTGA
TCTTTTCTAC
GGGGTCTGAC
GCTCAGTGGA
ACGAAAACTC
ACGTTAAGGG
ATTTTGGTCA
TGAGATTATC
AAAAAGGATC
TTCACCTAGA
TCCTTTTAAA
TTAAAAATGA
AGTTTTAAAT
CAATCTAAAG
TATATATGAG
TAAACTTGGT
CTGACAGTTA
CCAATGCTTA
ATCAGTGAGG
CACCTATCTC
AGCGATCTGT
CTATTTCGTT
CATCCATAGT
TGCCTGACTC
CCCGTCGTGT
AGATAACTAC
GATACGGGAG
GGCTTACCAT
CTGGCCCCAG
TGCTGCAATG
ATACCGCGAG
ACCCACGCTC
ACCGGCTCCA
GATTTATCAG
CAATAAACCA
GCCAGCCGGA
AGGGCCGAGC
GCAGAAGTGG
TCCTGCAACT
TTATCCGCCT
CCATCCAGTC
TATTAATTGT
TGCCGGGAAG
CTAGAGTAAG
TAGTTCGCCA
GTTAATAGTT
TGCGCAACGT
TGTTGCCATT
GCTACAGGCA
TCGTGGTGTC
ACGCTCGTCG
TTTGGTATGG
CTTCATTCAG
CTCCGGTTCC
CAACGATCAA
GGCGAGTTAC
ATGATCCCCC
ATGTTGTGCA
AAAAAGCGGT
TAGCTCCTTC
GGTCCTCCGA
TCGTTGTCAG
AAGTAAGTTG
GCCGCAGTGT
TATCACTCAT
GGTTATGGCA
GCACTGCATA
ATTCTCTTAC
TGTCATGCCA
TCCGTAAGAT
GCTTTTCTGT
GACTGGTGAG
TACTCAACCA
AGTCATTCTG
AGAATAGTGT
ATGCGGCGAC
CGAGTTGCTC
TTGCCCGGCG
TCAATACGGG
ATAATACCGC
GCCACATAGC
AGAACTTTAA
AAGTGCTCAT
CATTGGAAAA
CGTTCTTCGG
GGCGAAAACT
CTCAAGGATC
TTACCGCTGT
TGAGATCCAG
TTCGATGTAA
CCCACTCGTG
CACCCAACTG
ATCTTCAGCA
TCTTTTACTT
TCACCAGCGT
TTCTGGGTGA
GCAAAAACAG
GAAGGCAAAA
TGCCGCAAAA
AAGGGAATAA
GGGCGACACG
GAAATGTTGA
ATACTCATAC
TCTTCCTTTT
TCAATATTAT
TGAAGCATTT
ATCAGGGTTA
TTGTCTCATG
AGCGGATACA
TATTTGAATG
TATTTAGAAA
AATAAACAAA
TAGGGGTTCC
GCGCACATTT
CCCCGAAAAG
TGCCACCTGA
CGTCGACGGA
TCGGGAGATC
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TATCATGTCT
GGATCAACTG
GATAACTCAA
GCTAACCAAA
ATCATCCCAA
ACTTCCCACC
CCATACCCTA
TTACCACTGC
CAATTACCTA
GTGGTTTCAT
TTACTCTAAA
CCTGTGATTC
CTCTGAATTA
TTTTCATTTT
AAAGAAATTG
TATTTGTTAA
ATATGTACTA
CAAACTTAGT
AGTTTTTAAA
GAAATTGTAT
TTGTTAAATA
TGTACTACAA
ACTTAGTAGT