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pCaEXP-HOG1(C. albicans)

产品货号:P57542

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pCaEXP-HOG1(C. albicans)
别称: XM_715923.1
基因长:
质粒宿主: 丝状真菌
质粒用途: 蛋白表达
片段类型: ORF
片段物种: 白假丝酵母菌
原核抗性: Amp
筛选标记:
荧光标记:
启动子:
复制子:
拷贝数:
感受态: DH5a
温度: 37度
背景载体:
正向引物: pCaEXP-F1
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

TCAGCAATAA

ACCAGCCAGC

CGGAAGGGCC

GAGCGCAGAA

GTGGTCCTGC

AACTTTATCC

61

GCCTCCATCC

AGTCTATTAA

TTGTTGCCGG

GAAGCTAGAG

TAAGTAGTTC

GCCAGTTAAT

121

AGTTTGCGCA

ACGTTGTTGC

CATTGCTACA

GGCATCGTGG

TGTCACGCTC

GTCGTTTGGT

181

ATGGCTTCAT

TCAGCTCCGG

TTCCCAACGA

TCAAGGCGAG

TTACATGATC

CCCCATGTTG

241

TGCAAAAAAG

CGGTTAGCTC

CTTCGGTCCT

CCGATCGTTG

TCAGAAGTAA

GTTGGCCGCA

301

GTGTTATCAC

TCATGGTTAT

GGCAGCACTG

CATAATTCTC

TTACTGTCAT

GCCATCCGTA

361

AGATGCTTTT

CTGTGACTGG

TGAGTACTCA

ACCAAGTCAT

TCTGAGAATA

GTGTATGCGG

421

CGACCGAGTT

GCTCTTGCCC

GGCGTCAATA

CGGGATAATA

CCGCGCCACA

TAGCAGAACT

481

TTAAAAGTGC

TCATCATTGG

AAAACGTTCT

TCGGGGCGAA

AACTCTCAAG

GATCTTACCG

541

CTGTTGAGAT

CCAGTTCGAT

GTAACCCACT

CGTGCACCCA

ACTGATCTTC

AGCATCTTTT

601

ACTTTCACCA

GCGTTTCTGG

GTGAGCAAAA

ACAGGAAGGC

AAAATGCCGC

AAAAAAGGGA

661

ATAAGGGCGA

CACGGAAATG

TTGAATACTC

ATACTCTTCC

TTTTTCAATA

TTATTGAAGC

721

ATTTATCAGG

GTTATTGTCT

CATGAGCGGA

TACATATTTG

AATGTATTTA

GAAAAATAAA

781

CAAATAGGGG

TTCCGCGCAC

ATTTCCCCGA

AAAGTGCCAC

CTGACGTCTA

AGAAACCATT

841

ATTATCATGA

CATTAACCTA

TAAAAATAGG

CGTATCACGA

GGCCCTTTCG

TCTCGCGCGT

901

TTCGGTGATG

ACGGTGAAAA

CCTCTGACAC

ATGCAGCTCC

CGGAGACGGT

CACAGCTTGT

961

CTGTAAGCGG

ATGCCGGGAG

CAGACAAGCC

CGTCAGGGCG

CGTCAGCGGG

TGTTGGCGGG

1021

TGTCGGGGCT

GGCTTAACTA

TGCGGCATCA

GAGCAGATTG

TACTGAGAGT

GCACCATATG

1081

CAGATCCTTA

TCCTTAAACA

GATTCTAAAA

CAACATCTTT

GAAACCAGAA

GAAACTTTCT

1141

TACCTTTTTC

TTCGGTTGAA

CCTTCACCGT

GCAAAGCCAA

TAATGAACCC

AAGTCGAATT

1201

TTGGTTGTTT

CAACAATTTG

ACTTTTCTGA

TGTGGACATT

TTGTAATGGG

AAAATGGTTT

1261

GGGTGGATTT

TTCAATTTCA

CGGCCAATGA

CTTCTGGAAT

CAATTTGGAA

GTTAATTGAG

1321

CTAAAGTACA

GTTGGAAACT

TCTCTTTGCA

TAATTTCAAT

CATCTTCTTT

CTGACTTCTC

1381

TCAATTTAGA

GGATTGAGCG

TGAGTAGTTT

TCTTGATTTG

GTTTGGTTGT

CTTTTGGTGA

1441

AAGCAATGGC

AAAAACTCTC

AAAACGTAAT

CGTCGGAAGT

TTTAACAGTG

ACATTAGCTT

1501

CAACTAATGA

TTGCCATTTT

CTGACCAATG

ATCTTAATTT

GTCAGAAGTG

AAATCCAAAC

1561

CATGAGGCCT

CCATGAGGCC

TCCATGAGGC

CTCCATGAGG

CCTCCATGGA

GGCCTCATGG

1621

AAGTTGGTCA

ACAAGTTTTT

ACCTTGGACT

TCATCAACTC

TCAATTTGAT

TTTTCTGTAA

1681

GAGTGGTCTT

CGGAACCTTG

TAAGTCGGCC

AAACAAACTT

CGAAAACTCT

ACCTTTCAAG

1741

CCATCAGCGG

CATTCTTTAA

ACCGGTAGAT

CTGTTGATCA

AAGTTTTACC

AACATTTCTG

1801

TTTTCAAAAG

TGGTTGGAGC

TTTGATGTCA

AACCAATCTT

TTCTGGTGAA

TGGGTCAACG

1861

ACCTTCTTTT

TTAATCCTTT

CTTTCCTTTG

GACAATCTCT

TGTTTTTACC

GACAGCCATG

1921

TTGTACTTGC

ATATGCGGTG

TGAAATACCG

CACAGATGCG

TAAGGAGAAA

ATACCGCATC

1981

AGGCGCCATT

CGCCATTCAG

GCTGCGCAAC

TGTTGGGAAG

GGCGATCGGT

GCGGGCCTCT

2041

TCGCTATTAC

GCCAGCTGGC

GAAAGGGGGA

TGTGCTGCAA

GGCGATTAAG

TTGGGTAACG

2101

CCAGGGTTTT

CCCAGTCACG

ACGTTGTAAA

ACGACGGCCA

GTGAATTGTC

TATTCCAAGC

2161

CTGTGGCTTT

TTTTGGTGAT

TCACAATACA

TAAATTGGCA

AATACCTCTT

CTATAGACTT

2221

TAACCTTTGA

TGAAATGGTA

TGGATAAACC

ACTTTTGTGG

TCAGGGTATA

TATATTTTTA

2281

TTCCAATCTG

GCCTTTATAA

ATTCAAATTT

CCAAGGGGAC

TCTGCTAAAC

CTTCTGATAT

2341

AACTATAACT

CTAATAATGG

CTGTAAAAAG

TGTGAAAAAA

AGATGATATT

CACAAAAAAG

2401

ATAAAGGGGA

TTTCATATTG

GGTATCTAGA

TTTAAACATC

GTATTTCAAC

AATGAAACTA

2461

GAATCAATAC

GAAATCCAGG

GCACAACTGT

GTTCATTCCC

TTGGGAATTA

TACTGAGTAA

2521

TGGGAAGAAT

GTGCAAAAAA

TACCCGTGTC

TTATATAATT

TCCATTTGTG

TAGGGCTTAA

2581

AAGTGGACAA

CTTCAGGTTA

CGAAACCACG

AAAATGTCTA

AACGTTTGAT

CAAATTCGTA

2641

ACTCATGGAA

AATCACAGCA

ACAAATTAAG

GATGGATATA

TCCGGTACTT

ACAAATAAAA

2701

ACACTAAAGA

AGTATAAATT

TTGAACTGAA

ATTCTATAGA

AAATCTTTTT

TTTTTGGGTA

2761

AAATTATGCG

ATTGTGGCTC

ATAGTAACGT

AATCATACAG

TTCAACAAAC

ATCATTTTGT

2821

CAAAGGATTT

CTAATCCCAT

TCTTTTTCTA

CTTGGCTTTT

GAGTATATAT

ATATATATAC

2881

ATCCTTTGTT

CATCATCATG

TTCGATTGAA

TTTTTTTCTT

GGTTGTTATT

AGATTGTCTA

2941

CTTGAGTTTT

TCTTGGTGCA

AATTGTGGTG

TTTTTCTAAC

AAATTTTTCA

ACATTTAAAG

3001

TATTTCTGGA

ACTTTTCTAC

AAGGAACGAC

ATAGATCCAT

TAATGCGCCC

CTCTAAAACA

3061

ATACCCATAA

TGTCTTCTTA

AGTATGCATC

TGCTTATAAA

TGTTTCTGGA

TTTACTTTAG

3121

TTAGAATGTC

GACACGACAA

TGAACTATTG

CTGTGGATCA

CGTGCGATCA

CTGAATTATG

3181

AGTTTCTTTT

TATTTTTCTT

TTTGGGCTTT

TTTTTGCAAT

TTTTTTTTTC

CACCTTTGCT

3241

AATTGCGTAG

TCACATATAA

TGTATACAAT

TTTTGCAACT

CTGACACTAT

AATTGATCCA

3301

ATAATAAAGT

ATATAATAGA

TCATCAAGTA

TACGTAATCT

CCCCAAAAAA

AAAATTGCAT

3361

AAAATAAAAT

CTATAAACCT

TTTTTAGATT

TTTAGTTGTT

CCTTTTTTCT

TTCTATTCTT

3421

CAACTTCCAA

GTGTTGTCAC

TTTCTTCCTG

TTAACTATAT

TTAAAAGTAA

ATACCCTCCC

3481

CGGATCCATG

TCTGCAGATG

GAGAATTTAC

AAGAACCCAG

ATATTTGGGA

CTGTTTTTGA

3541

AATCACCAAT

AGATACACTG

AGCTAAATCC

CGTGGGAATG

GGAGCATTTG

GTTTGGTGTG

3601

CTCAGCCGTT

GATAGATTAA

CTGGTCAAAA

TGTTGCAGTG

AAAAAAGTCA

TGAAACCTTT

3661

TCTGACCTCA

GTATTGGCAA

AAAGAACCTA

CAGAGAATTA

AAGTTATTGA

AACATTTGAA

3721

ACATGAGAAT

TTGATTACCT

TGGATGATAT

TTTCATATCT

CCTTTGGAGG

ATATTTACTT

3781

TGTCAATGAA

TTGCAAGGTA

CTGATTTGCA

TAGGTTATTG

AACTCAAGAC

CTTTGGAGAA

3841

ACAATTTATT

CAATATTTCA

CATACCAAAT

AATGAGAGGT

TTAAAATACA

TTCATTCTGC

3901

CGGTGTTATT

CATCGTGATT

TGAAACCATC

CAACATCTTG

ATCAATGAAA

ACTGTGACTT

3961

GAAAATCTGT

GACTTTGGTC

TTGCAAGACT

TCAAGATCCA

CAAATGACGG

GTTACGTGTC

4021

AACCAGATAT

TATCGAGCAC

CAGAAATCAT

GTTAACTTGG

CAAAAATACG

ACACTGAAGT

4081

CGACTTGTGG

TCTGTGGGTT

GTATCTTAGC

GGAAATGATT

GAAGGGAAAC

CACTATTCCC

4141

CGGGAAAGAT

CACGTGCATC

AATTCTCCAT

TATAACAGAA

TTATTGGGCT

CACCTCCTGC

4201

TGATGTGATC

GATACTATTT

GTTCCGAAAA

CACTTTAAGA

TTTGTCCAAT

CATTACCACA

4261

TAGAGACCCT

ATCCCATTTA

GTGAAAGATT

TGCTTCCTGT

ACTCACGTTG

AACCGGAGGC

4321

TATTGATTTA

TTGGCTAAAT

TGTTGGTTTT

TGACCCTAAG

AAAAGAATCA

GTGCTGTTGA

4381

AGGTTTGACC

CATCCTTATA

TGGAGGCATA

CCATGATCCA

ACCGATGAGC

CTGTTTGTGA

4441

GAGTAAATTC

GATTGGAGTT

TTAATGACGC

AGACTTGCCA

GTGGATACTT

GGAGAGTTAT

4501

GATGTACAGT

GAAATTTTAG

ACTTTCATCA

AACTGTTGGT

GTGGCAAATG

AAACTGAGGG

4561

TTCCGAACAA

CCAGACCTGC

AAGTGGAGCA

AAACAACTTG

GATTCCGCCA

ACGGAGCTTA

4621

AGCATGCAAG

CTTCCGGATA

ATAGGAATTG

ATTTGGATGG

TATAAACGGA

AACAAAAAAA

4681

AGAGCTGGTA

CTACTTTCTT

TAAAATTATT

TTATTATTTG

ATTTTATTTA

ATAGTATATA

4741

TTATATTTTG

AACGTAGATT

ATTTTGTTGA

AAGTTGCTGT

AGTGCCATTG

ATTCGTAACA

4801

CTAATTCTGT

ATTAGTCATT

CCTCTTGTTT

GATAGTATCC

AAAAAAACGG

CTATTTTTTT

4861

GCAATCTTAT

TTCCTGCATA

TTATACAGAT

AACATAATGA

AAGAAAAAAT

CTTTTTTTTT

4921

GTTCTTCAAT

GATGATTTCA

ACCATTCTTT

TAAACATTGA

TCAATTCCTG

AGCAACAACC

4981

CCATACACAC

TGGTTTATAT

ATCGCCCCTT

TTACAGTTGA

AGAAAGAAAT

AGAAATAGAA

5041

ATAGCAAACA

AAAGATATGA

CAGTCAACAC

TAAGACCTAT

AGTGAGAGAG

CAGAAACTCA

5101

TGCCTCACCA

GTAGCACAAC

GATTATTTCG

ATTAATGGAA

CTGAAGAAAA

CCAATTTATG

5161

TGCATCAATT

GATGTTGATA

CCACTAAGGA

ATTCCTTGAA

TTAATTGATA

AATTGGGTCC

5221

TTATGTATGC

TTAATCAAGA

CTCATATTGA

TATAATCAAT

GATTTTTCCT

ATGAATCCAC

5281

TATTGAACCA

TTATTAGAAC

TTTCACGTAA

ACATCAATTT

ATGATTTTTG

AAGATAGAAA

5341

ATTTGCTGAT

ATTGGTAATA

CCGTGAAGAA

ACAATATATT

GGTGGAGTTT

ATAAAATTAG

5401

TAGTTGGGCA

GATATTACTA

ATGCTCATGG

TGTCACTGGG

AATGGAGTAG

TTGAAGGATT

5461

AAAACAGGGA

GCTAAAGAAA

CCACCACCAA

CCAAGAGCCA

AGAGGGTTAT

TGATGTTAGC

5521

TGAATTATCA

TCAGTGGGAT

CATTAGCATA

TGGAGAATAT

TCTCAAAAAA

CTGTTGAAAT

5581

TGCTAAATCC

GATAAGGAAT

TTGTTATTGG

ATTTATTGCC

CAACGTGATA

TGGGTGGACA

5641

AGAAGAAGGA

TTTGATTGGC

TTATTATGAC

ACCTGGAGTT

GGATTAGATG

ATAAAGGTGA

5701

TGGATTAGGA

CAACAATATA

GAACTGTTGA

TGAAGTTGTT

AGCACTGGAA

CTGATATTAT

5761

CATTGTTGGT

AGAGGATTGT

TTGGTAAAGG

AAGAGATCCA

GATATTGAAG

GTAAAAGGTA

5821

TAGAGATGCT

GGTTGGAATG

CTTATTTGAA

AAAAACTGGC

CAATTATAAA

TGTGAAGGGG

5881

GAGATTTTCA

CTTTATTAGA

TTTGTATATA

TGTATAATAA

ATAAATAAAT

AAGTTAAATA

5941

AATAATTAAA

TAAGGGTGGT

AATTATTACT

ATTTACAATC

AAAGGTGGTC

CTTCTAAGCT

6001

TGGCGTAATC

ATGGTCATAG

CTGTTTCCTG

TGTGAAATTG

TTATCCGCTC

ACAATTCCAC

6061

ACAACATACG

AGCCGGAAGC

ATAAAGTGTA

AAGCCTGGGG

TGCCTAATGA

GTGAGCTAAC

6121

TCACATTAAT

TGCGTTGCGC

TCACTGCCCG

CTTTCCAGTC

GGGAAACCTG

TCGTGCCAGC

6181

TGCATTAATG

AATCGGCCAA

CGCGCGGGGA

GAGGCGGTTT

GCGTATTGGG

CGCTCTTCCG

6241

CTTCCTCGCT

CACTGACTCG

CTGCGCTCGG

TCGTTCGGCT

GCGGCGAGCG

GTATCAGCTC

6301

ACTCAAAGGC

GGTAATACGG

TTATCCACAG

AATCAGGGGA

TAACGCAGGA

AAGAACATGT

6361

GAGCAAAAGG

CCAGCAAAAG

GCCAGGAACC

GTAAAAAGGC

CGCGTTGCTG

GCGTTTTTCC

6421

ATAGGCTCCG

CCCCCCTGAC

GAGCATCACA

AAAATCGACG

CTCAAGTCAG

AGGTGGCGAA

6481

ACCCGACAGG

ACTATAAAGA

TACCAGGCGT

TTCCCCCTGG

AAGCTCCCTC

GTGCGCTCTC

6541

CTGTTCCGAC

CCTGCCGCTT

ACCGGATACC

TGTCCGCCTT

TCTCCCTTCG

GGAAGCGTGG

6601

CGCTTTCTCA

TAGCTCACGC

TGTAGGTATC

TCAGTTCGGT

GTAGGTCGTT

CGCTCCAAGC

6661

TGGGCTGTGT

GCACGAACCC

CCCGTTCAGC

CCGACCGCTG

CGCCTTATCC

GGTAACTATC

6721

GTCTTGAGTC

CAACCCGGTA

AGACACGACT

TATCGCCACT

GGCAGCAGCC

ACTGGTAACA

6781

GGATTAGCAG

AGCGAGGTAT

GTAGGCGGTG

CTACAGAGTT

CTTGAAGTGG

TGGCCTAACT

6841

ACGGCTACAC

TAGAAGAACA

GTATTTGGTA

TCTGCGCTCT

GCTGAAGCCA

GTTACCTTCG

6901

GAAAAAGAGT

TGGTAGCTCT

TGATCCGGCA

AACAAACCAC

CGCTGGTAGC

GGTGGTTTTT

6961

TTGTTTGCAA

GCAGCAGATT

ACGCGCAGAA

AAAAAGGATC

TCAAGAAGAT

CCTTTGATCT

7021

TTTCTACGGG

GTCTGACGCT

CAGTGGAACG

AAAACTCACG

TTAAGGGATT

TTGGTCATGA

7081

GATTATCAAA

AAGGATCTTC

ACCTAGATCC

TTTTAAATTA

AAAATGAAGT

TTTAAATCAA

7141

TCTAAAGTAT

ATATGAGTAA

ACTTGGTCTG

ACAGTTACCA

ATGCTTAATC

AGTGAGGCAC

7201

CTATCTCAGC

GATCTGTCTA

TTTCGTTCAT

CCATAGTTGC

CTGACTCCCC

GTCGTGTAGA

7261

TAACTACGAT

ACGGGAGGGC

TTACCATCTG

GCCCCAGTGC

TGCAATGATA

CCGCGAGACC

7321

CACGCTCACC

GGCTCCAGAT

TTATCAGCAA

TAAACCAGCC

AGCCGGAAGG

CCGAAGCGGC

7381

AGAAGTGGTC

CTGCAACTTT

ATCCGCCT

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