名称: | pCaEXP-HOG1(C. albicans) |
---|---|
别称: | XM_715923.1 |
基因长: | |
质粒宿主: | 丝状真菌 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 白假丝酵母菌 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | |
复制子: | |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | pCaEXP-F1 |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TCAGCAATAA
ACCAGCCAGC
CGGAAGGGCC
GAGCGCAGAA
GTGGTCCTGC
AACTTTATCC
GCCTCCATCC
AGTCTATTAA
TTGTTGCCGG
GAAGCTAGAG
TAAGTAGTTC
GCCAGTTAAT
AGTTTGCGCA
ACGTTGTTGC
CATTGCTACA
GGCATCGTGG
TGTCACGCTC
GTCGTTTGGT
ATGGCTTCAT
TCAGCTCCGG
TTCCCAACGA
TCAAGGCGAG
TTACATGATC
CCCCATGTTG
TGCAAAAAAG
CGGTTAGCTC
CTTCGGTCCT
CCGATCGTTG
TCAGAAGTAA
GTTGGCCGCA
GTGTTATCAC
TCATGGTTAT
GGCAGCACTG
CATAATTCTC
TTACTGTCAT
GCCATCCGTA
AGATGCTTTT
CTGTGACTGG
TGAGTACTCA
ACCAAGTCAT
TCTGAGAATA
GTGTATGCGG
CGACCGAGTT
GCTCTTGCCC
GGCGTCAATA
CGGGATAATA
CCGCGCCACA
TAGCAGAACT
TTAAAAGTGC
TCATCATTGG
AAAACGTTCT
TCGGGGCGAA
AACTCTCAAG
GATCTTACCG
CTGTTGAGAT
CCAGTTCGAT
GTAACCCACT
CGTGCACCCA
ACTGATCTTC
AGCATCTTTT
ACTTTCACCA
GCGTTTCTGG
GTGAGCAAAA
ACAGGAAGGC
AAAATGCCGC
AAAAAAGGGA
ATAAGGGCGA
CACGGAAATG
TTGAATACTC
ATACTCTTCC
TTTTTCAATA
TTATTGAAGC
ATTTATCAGG
GTTATTGTCT
CATGAGCGGA
TACATATTTG
AATGTATTTA
GAAAAATAAA
CAAATAGGGG
TTCCGCGCAC
ATTTCCCCGA
AAAGTGCCAC
CTGACGTCTA
AGAAACCATT
ATTATCATGA
CATTAACCTA
TAAAAATAGG
CGTATCACGA
GGCCCTTTCG
TCTCGCGCGT
TTCGGTGATG
ACGGTGAAAA
CCTCTGACAC
ATGCAGCTCC
CGGAGACGGT
CACAGCTTGT
CTGTAAGCGG
ATGCCGGGAG
CAGACAAGCC
CGTCAGGGCG
CGTCAGCGGG
TGTTGGCGGG
TGTCGGGGCT
GGCTTAACTA
TGCGGCATCA
GAGCAGATTG
TACTGAGAGT
GCACCATATG
CAGATCCTTA
TCCTTAAACA
GATTCTAAAA
CAACATCTTT
GAAACCAGAA
GAAACTTTCT
TACCTTTTTC
TTCGGTTGAA
CCTTCACCGT
GCAAAGCCAA
TAATGAACCC
AAGTCGAATT
TTGGTTGTTT
CAACAATTTG
ACTTTTCTGA
TGTGGACATT
TTGTAATGGG
AAAATGGTTT
GGGTGGATTT
TTCAATTTCA
CGGCCAATGA
CTTCTGGAAT
CAATTTGGAA
GTTAATTGAG
CTAAAGTACA
GTTGGAAACT
TCTCTTTGCA
TAATTTCAAT
CATCTTCTTT
CTGACTTCTC
TCAATTTAGA
GGATTGAGCG
TGAGTAGTTT
TCTTGATTTG
GTTTGGTTGT
CTTTTGGTGA
AAGCAATGGC
AAAAACTCTC
AAAACGTAAT
CGTCGGAAGT
TTTAACAGTG
ACATTAGCTT
CAACTAATGA
TTGCCATTTT
CTGACCAATG
ATCTTAATTT
GTCAGAAGTG
AAATCCAAAC
CATGAGGCCT
CCATGAGGCC
TCCATGAGGC
CTCCATGAGG
CCTCCATGGA
GGCCTCATGG
AAGTTGGTCA
ACAAGTTTTT
ACCTTGGACT
TCATCAACTC
TCAATTTGAT
TTTTCTGTAA
GAGTGGTCTT
CGGAACCTTG
TAAGTCGGCC
AAACAAACTT
CGAAAACTCT
ACCTTTCAAG
CCATCAGCGG
CATTCTTTAA
ACCGGTAGAT
CTGTTGATCA
AAGTTTTACC
AACATTTCTG
TTTTCAAAAG
TGGTTGGAGC
TTTGATGTCA
AACCAATCTT
TTCTGGTGAA
TGGGTCAACG
ACCTTCTTTT
TTAATCCTTT
CTTTCCTTTG
GACAATCTCT
TGTTTTTACC
GACAGCCATG
TTGTACTTGC
ATATGCGGTG
TGAAATACCG
CACAGATGCG
TAAGGAGAAA
ATACCGCATC
AGGCGCCATT
CGCCATTCAG
GCTGCGCAAC
TGTTGGGAAG
GGCGATCGGT
GCGGGCCTCT
TCGCTATTAC
GCCAGCTGGC
GAAAGGGGGA
TGTGCTGCAA
GGCGATTAAG
TTGGGTAACG
CCAGGGTTTT
CCCAGTCACG
ACGTTGTAAA
ACGACGGCCA
GTGAATTGTC
TATTCCAAGC
CTGTGGCTTT
TTTTGGTGAT
TCACAATACA
TAAATTGGCA
AATACCTCTT
CTATAGACTT
TAACCTTTGA
TGAAATGGTA
TGGATAAACC
ACTTTTGTGG
TCAGGGTATA
TATATTTTTA
TTCCAATCTG
GCCTTTATAA
ATTCAAATTT
CCAAGGGGAC
TCTGCTAAAC
CTTCTGATAT
AACTATAACT
CTAATAATGG
CTGTAAAAAG
TGTGAAAAAA
AGATGATATT
CACAAAAAAG
ATAAAGGGGA
TTTCATATTG
GGTATCTAGA
TTTAAACATC
GTATTTCAAC
AATGAAACTA
GAATCAATAC
GAAATCCAGG
GCACAACTGT
GTTCATTCCC
TTGGGAATTA
TACTGAGTAA
TGGGAAGAAT
GTGCAAAAAA
TACCCGTGTC
TTATATAATT
TCCATTTGTG
TAGGGCTTAA
AAGTGGACAA
CTTCAGGTTA
CGAAACCACG
AAAATGTCTA
AACGTTTGAT
CAAATTCGTA
ACTCATGGAA
AATCACAGCA
ACAAATTAAG
GATGGATATA
TCCGGTACTT
ACAAATAAAA
ACACTAAAGA
AGTATAAATT
TTGAACTGAA
ATTCTATAGA
AAATCTTTTT
TTTTTGGGTA
AAATTATGCG
ATTGTGGCTC
ATAGTAACGT
AATCATACAG
TTCAACAAAC
ATCATTTTGT
CAAAGGATTT
CTAATCCCAT
TCTTTTTCTA
CTTGGCTTTT
GAGTATATAT
ATATATATAC
ATCCTTTGTT
CATCATCATG
TTCGATTGAA
TTTTTTTCTT
GGTTGTTATT
AGATTGTCTA
CTTGAGTTTT
TCTTGGTGCA
AATTGTGGTG
TTTTTCTAAC
AAATTTTTCA
ACATTTAAAG
TATTTCTGGA
ACTTTTCTAC
AAGGAACGAC
ATAGATCCAT
TAATGCGCCC
CTCTAAAACA
ATACCCATAA
TGTCTTCTTA
AGTATGCATC
TGCTTATAAA
TGTTTCTGGA
TTTACTTTAG
TTAGAATGTC
GACACGACAA
TGAACTATTG
CTGTGGATCA
CGTGCGATCA
CTGAATTATG
AGTTTCTTTT
TATTTTTCTT
TTTGGGCTTT
TTTTTGCAAT
TTTTTTTTTC
CACCTTTGCT
AATTGCGTAG
TCACATATAA
TGTATACAAT
TTTTGCAACT
CTGACACTAT
AATTGATCCA
ATAATAAAGT
ATATAATAGA
TCATCAAGTA
TACGTAATCT
CCCCAAAAAA
AAAATTGCAT
AAAATAAAAT
CTATAAACCT
TTTTTAGATT
TTTAGTTGTT
CCTTTTTTCT
TTCTATTCTT
CAACTTCCAA
GTGTTGTCAC
TTTCTTCCTG
TTAACTATAT
TTAAAAGTAA
ATACCCTCCC
CGGATCCATG
TCTGCAGATG
GAGAATTTAC
AAGAACCCAG
ATATTTGGGA
CTGTTTTTGA
AATCACCAAT
AGATACACTG
AGCTAAATCC
CGTGGGAATG
GGAGCATTTG
GTTTGGTGTG
CTCAGCCGTT
GATAGATTAA
CTGGTCAAAA
TGTTGCAGTG
AAAAAAGTCA
TGAAACCTTT
TCTGACCTCA
GTATTGGCAA
AAAGAACCTA
CAGAGAATTA
AAGTTATTGA
AACATTTGAA
ACATGAGAAT
TTGATTACCT
TGGATGATAT
TTTCATATCT
CCTTTGGAGG
ATATTTACTT
TGTCAATGAA
TTGCAAGGTA
CTGATTTGCA
TAGGTTATTG
AACTCAAGAC
CTTTGGAGAA
ACAATTTATT
CAATATTTCA
CATACCAAAT
AATGAGAGGT
TTAAAATACA
TTCATTCTGC
CGGTGTTATT
CATCGTGATT
TGAAACCATC
CAACATCTTG
ATCAATGAAA
ACTGTGACTT
GAAAATCTGT
GACTTTGGTC
TTGCAAGACT
TCAAGATCCA
CAAATGACGG
GTTACGTGTC
AACCAGATAT
TATCGAGCAC
CAGAAATCAT
GTTAACTTGG
CAAAAATACG
ACACTGAAGT
CGACTTGTGG
TCTGTGGGTT
GTATCTTAGC
GGAAATGATT
GAAGGGAAAC
CACTATTCCC
CGGGAAAGAT
CACGTGCATC
AATTCTCCAT
TATAACAGAA
TTATTGGGCT
CACCTCCTGC
TGATGTGATC
GATACTATTT
GTTCCGAAAA
CACTTTAAGA
TTTGTCCAAT
CATTACCACA
TAGAGACCCT
ATCCCATTTA
GTGAAAGATT
TGCTTCCTGT
ACTCACGTTG
AACCGGAGGC
TATTGATTTA
TTGGCTAAAT
TGTTGGTTTT
TGACCCTAAG
AAAAGAATCA
GTGCTGTTGA
AGGTTTGACC
CATCCTTATA
TGGAGGCATA
CCATGATCCA
ACCGATGAGC
CTGTTTGTGA
GAGTAAATTC
GATTGGAGTT
TTAATGACGC
AGACTTGCCA
GTGGATACTT
GGAGAGTTAT
GATGTACAGT
GAAATTTTAG
ACTTTCATCA
AACTGTTGGT
GTGGCAAATG
AAACTGAGGG
TTCCGAACAA
CCAGACCTGC
AAGTGGAGCA
AAACAACTTG
GATTCCGCCA
ACGGAGCTTA
AGCATGCAAG
CTTCCGGATA
ATAGGAATTG
ATTTGGATGG
TATAAACGGA
AACAAAAAAA
AGAGCTGGTA
CTACTTTCTT
TAAAATTATT
TTATTATTTG
ATTTTATTTA
ATAGTATATA
TTATATTTTG
AACGTAGATT
ATTTTGTTGA
AAGTTGCTGT
AGTGCCATTG
ATTCGTAACA
CTAATTCTGT
ATTAGTCATT
CCTCTTGTTT
GATAGTATCC
AAAAAAACGG
CTATTTTTTT
GCAATCTTAT
TTCCTGCATA
TTATACAGAT
AACATAATGA
AAGAAAAAAT
CTTTTTTTTT
GTTCTTCAAT
GATGATTTCA
ACCATTCTTT
TAAACATTGA
TCAATTCCTG
AGCAACAACC
CCATACACAC
TGGTTTATAT
ATCGCCCCTT
TTACAGTTGA
AGAAAGAAAT
AGAAATAGAA
ATAGCAAACA
AAAGATATGA
CAGTCAACAC
TAAGACCTAT
AGTGAGAGAG
CAGAAACTCA
TGCCTCACCA
GTAGCACAAC
GATTATTTCG
ATTAATGGAA
CTGAAGAAAA
CCAATTTATG
TGCATCAATT
GATGTTGATA
CCACTAAGGA
ATTCCTTGAA
TTAATTGATA
AATTGGGTCC
TTATGTATGC
TTAATCAAGA
CTCATATTGA
TATAATCAAT
GATTTTTCCT
ATGAATCCAC
TATTGAACCA
TTATTAGAAC
TTTCACGTAA
ACATCAATTT
ATGATTTTTG
AAGATAGAAA
ATTTGCTGAT
ATTGGTAATA
CCGTGAAGAA
ACAATATATT
GGTGGAGTTT
ATAAAATTAG
TAGTTGGGCA
GATATTACTA
ATGCTCATGG
TGTCACTGGG
AATGGAGTAG
TTGAAGGATT
AAAACAGGGA
GCTAAAGAAA
CCACCACCAA
CCAAGAGCCA
AGAGGGTTAT
TGATGTTAGC
TGAATTATCA
TCAGTGGGAT
CATTAGCATA
TGGAGAATAT
TCTCAAAAAA
CTGTTGAAAT
TGCTAAATCC
GATAAGGAAT
TTGTTATTGG
ATTTATTGCC
CAACGTGATA
TGGGTGGACA
AGAAGAAGGA
TTTGATTGGC
TTATTATGAC
ACCTGGAGTT
GGATTAGATG
ATAAAGGTGA
TGGATTAGGA
CAACAATATA
GAACTGTTGA
TGAAGTTGTT
AGCACTGGAA
CTGATATTAT
CATTGTTGGT
AGAGGATTGT
TTGGTAAAGG
AAGAGATCCA
GATATTGAAG
GTAAAAGGTA
TAGAGATGCT
GGTTGGAATG
CTTATTTGAA
AAAAACTGGC
CAATTATAAA
TGTGAAGGGG
GAGATTTTCA
CTTTATTAGA
TTTGTATATA
TGTATAATAA
ATAAATAAAT
AAGTTAAATA
AATAATTAAA
TAAGGGTGGT
AATTATTACT
ATTTACAATC
AAAGGTGGTC
CTTCTAAGCT
TGGCGTAATC
ATGGTCATAG
CTGTTTCCTG
TGTGAAATTG
TTATCCGCTC
ACAATTCCAC
ACAACATACG
AGCCGGAAGC
ATAAAGTGTA
AAGCCTGGGG
TGCCTAATGA
GTGAGCTAAC
TCACATTAAT
TGCGTTGCGC
TCACTGCCCG
CTTTCCAGTC
GGGAAACCTG
TCGTGCCAGC
TGCATTAATG
AATCGGCCAA
CGCGCGGGGA
GAGGCGGTTT
GCGTATTGGG
CGCTCTTCCG
CTTCCTCGCT
CACTGACTCG
CTGCGCTCGG
TCGTTCGGCT
GCGGCGAGCG
GTATCAGCTC
ACTCAAAGGC
GGTAATACGG
TTATCCACAG
AATCAGGGGA
TAACGCAGGA
AAGAACATGT
GAGCAAAAGG
CCAGCAAAAG
GCCAGGAACC
GTAAAAAGGC
CGCGTTGCTG
GCGTTTTTCC
ATAGGCTCCG
CCCCCCTGAC
GAGCATCACA
AAAATCGACG
CTCAAGTCAG
AGGTGGCGAA
ACCCGACAGG
ACTATAAAGA
TACCAGGCGT
TTCCCCCTGG
AAGCTCCCTC
GTGCGCTCTC
CTGTTCCGAC
CCTGCCGCTT
ACCGGATACC
TGTCCGCCTT
TCTCCCTTCG
GGAAGCGTGG
CGCTTTCTCA
TAGCTCACGC
TGTAGGTATC
TCAGTTCGGT
GTAGGTCGTT
CGCTCCAAGC
TGGGCTGTGT
GCACGAACCC
CCCGTTCAGC
CCGACCGCTG
CGCCTTATCC
GGTAACTATC
GTCTTGAGTC
CAACCCGGTA
AGACACGACT
TATCGCCACT
GGCAGCAGCC
ACTGGTAACA
GGATTAGCAG
AGCGAGGTAT
GTAGGCGGTG
CTACAGAGTT
CTTGAAGTGG
TGGCCTAACT
ACGGCTACAC
TAGAAGAACA
GTATTTGGTA
TCTGCGCTCT
GCTGAAGCCA
GTTACCTTCG
GAAAAAGAGT
TGGTAGCTCT
TGATCCGGCA
AACAAACCAC
CGCTGGTAGC
GGTGGTTTTT
TTGTTTGCAA
GCAGCAGATT
ACGCGCAGAA
AAAAAGGATC
TCAAGAAGAT
CCTTTGATCT
TTTCTACGGG
GTCTGACGCT
CAGTGGAACG
AAAACTCACG
TTAAGGGATT
TTGGTCATGA
GATTATCAAA
AAGGATCTTC
ACCTAGATCC
TTTTAAATTA
AAAATGAAGT
TTTAAATCAA
TCTAAAGTAT
ATATGAGTAA
ACTTGGTCTG
ACAGTTACCA
ATGCTTAATC
AGTGAGGCAC
CTATCTCAGC
GATCTGTCTA
TTTCGTTCAT
CCATAGTTGC
CTGACTCCCC
GTCGTGTAGA
TAACTACGAT
ACGGGAGGGC
TTACCATCTG
GCCCCAGTGC
TGCAATGATA
CCGCGAGACC
CACGCTCACC
GGCTCCAGAT
TTATCAGCAA
TAAACCAGCC
AGCCGGAAGG
CCGAAGCGGC
AGAAGTGGTC
CTGCAACTTT
ATCCGCCT