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pCaEXP-PBS2(C. albicans)

产品货号:P57227

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pCaEXP-PBS2(C. albicans)
别称: XM_711536.1
基因长: 1638bp
质粒宿主: 丝状真菌
质粒用途: 蛋白表达
片段类型: ORF
片段物种: 念球菌
原核抗性: Amp
筛选标记:
荧光标记:
启动子:
复制子:
拷贝数:
感受态: DH5a
温度: 37度
背景载体:
正向引物:
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

TCAGCAATAA

ACCAGCCAGC

CGGAAGGGCC

GAGCGCAGAA

GTGGTCCTGC

AACTTTATCC

61

GCCTCCATCC

AGTCTATTAA

TTGTTGCCGG

GAAGCTAGAG

TAAGTAGTTC

GCCAGTTAAT

121

AGTTTGCGCA

ACGTTGTTGC

CATTGCTACA

GGCATCGTGG

TGTCACGCTC

GTCGTTTGGT

181

ATGGCTTCAT

TCAGCTCCGG

TTCCCAACGA

TCAAGGCGAG

TTACATGATC

CCCCATGTTG

241

TGCAAAAAAG

CGGTTAGCTC

CTTCGGTCCT

CCGATCGTTG

TCAGAAGTAA

GTTGGCCGCA

301

GTGTTATCAC

TCATGGTTAT

GGCAGCACTG

CATAATTCTC

TTACTGTCAT

GCCATCCGTA

361

AGATGCTTTT

CTGTGACTGG

TGAGTACTCA

ACCAAGTCAT

TCTGAGAATA

GTGTATGCGG

421

CGACCGAGTT

GCTCTTGCCC

GGCGTCAATA

CGGGATAATA

CCGCGCCACA

TAGCAGAACT

481

TTAAAAGTGC

TCATCATTGG

AAAACGTTCT

TCGGGGCGAA

AACTCTCAAG

GATCTTACCG

541

CTGTTGAGAT

CCAGTTCGAT

GTAACCCACT

CGTGCACCCA

ACTGATCTTC

AGCATCTTTT

601

ACTTTCACCA

GCGTTTCTGG

GTGAGCAAAA

ACAGGAAGGC

AAAATGCCGC

AAAAAAGGGA

661

ATAAGGGCGA

CACGGAAATG

TTGAATACTC

ATACTCTTCC

TTTTTCAATA

TTATTGAAGC

721

ATTTATCAGG

GTTATTGTCT

CATGAGCGGA

TACATATTTG

AATGTATTTA

GAAAAATAAA

781

CAAATAGGGG

TTCCGCGCAC

ATTTCCCCGA

AAAGTGCCAC

CTGACGTCTA

AGAAACCATT

841

ATTATCATGA

CATTAACCTA

TAAAAATAGG

CGTATCACGA

GGCCCTTTCG

TCTCGCGCGT

901

TTCGGTGATG

ACGGTGAAAA

CCTCTGACAC

ATGCAGCTCC

CGGAGACGGT

CACAGCTTGT

961

CTGTAAGCGG

ATGCCGGGAG

CAGACAAGCC

CGTCAGGGCG

CGTCAGCGGG

TGTTGGCGGG

1021

TGTCGGGGCT

GGCTTAACTA

TGCGGCATCA

GAGCAGATTG

TACTGAGAGT

GCACCATATG

1081

CAGATCCTTA

TCCTTAAACA

GATTCTAAAA

CAACATCTTT

GAAACCAGAA

GAAACTTTCT

1141

TACCTTTTTC

TTCGGTTGAA

CCTTCACCGT

GCAAAGCCAA

TAATGAACCC

AAGTCGAATT

1201

TTGGTTGTTT

CAACAATTTG

ACTTTTCTGA

TGTGGACATT

TTGTAATGGG

AAAATGGTTT

1261

GGGTGGATTT

TTCAATTTCA

CGGCCAATGA

CTTCTGGAAT

CAATTTGGAA

GTTAATTGAG

1321

CTAAAGTACA

GTTGGAAACT

TCTCTTTGCA

TAATTTCAAT

CATCTTCTTT

CTGACTTCTC

1381

TCAATTTAGA

GGATTGAGCG

TGAGTAGTTT

TCTTGATTTG

GTTTGGTTGT

CTTTTGGTGA

1441

AAGCAATGGC

AAAAACTCTC

AAAACGTAAT

CGTCGGAAGT

TTTAACAGTG

ACATTAGCTT

1501

CAACTAATGA

TTGCCATTTT

CTGACCAATG

ATCTTAATTT

GTCAGAAGTG

AAATCCAAAC

1561

CATGAGGCCT

CCATGAGGCC

TCCATGAGGC

CTCCATGAGG

CCTCCATGGA

GGCCTCATGG

1621

AAGTTGGTCA

ACAAGTTTTT

ACCTTGGACT

TCATCAACTC

TCAATTTGAT

TTTTCTGTAA

1681

GAGTGGTCTT

CGGAACCTTG

TAAGTCGGCC

AAACAAACTT

CGAAAACTCT

ACCTTTCAAG

1741

CCATCAGCGG

CATTCTTTAA

ACCGGTAGAT

CTGTTGATCA

AAGTTTTACC

AACATTTCTG

1801

TTTTCAAAAG

TGGTTGGAGC

TTTGATGTCA

AACCAATCTT

TTCTGGTGAA

TGGGTCAACG

1861

ACCTTCTTTT

TTAATCCTTT

CTTTCCTTTG

GACAATCTCT

TGTTTTTACC

GACAGCCATG

1921

TTGTACTTGC

ATATGCGGTG

TGAAATACCG

CACAGATGCG

TAAGGAGAAA

ATACCGCATC

1981

AGGCGCCATT

CGCCATTCAG

GCTGCGCAAC

TGTTGGGAAG

GGCGATCGGT

GCGGGCCTCT

2041

TCGCTATTAC

GCCAGCTGGC

GAAAGGGGGA

TGTGCTGCAA

GGCGATTAAG

TTGGGTAACG

2101

CCAGGGTTTT

CCCAGTCACG

ACGTTGTAAA

ACGACGGCCA

GTGAATTGTC

TATTCCAAGC

2161

CTGTGGCTTT

TTTTGGTGAT

TCACAATACA

TAAATTGGCA

AATACCTCTT

CTATAGACTT

2221

TAACCTTTGA

TGAAATGGTA

TGGATAAACC

ACTTTTGTGG

TCAGGGTATA

TATATTTTTA

2281

TTCCAATCTG

GCCTTTATAA

ATTCAAATTT

CCAAGGGGAC

TCTGCTAAAC

CTTCTGATAT

2341

AACTATAACT

CTAATAATGG

CTGTAAAAAG

TGTGAAAAAA

AGATGATATT

CACAAAAAAG

2401

ATAAAGGGGA

TTTCATATTG

GGTATCTAGA

TTTAAACATC

GTATTTCAAC

AATGAAACTA

2461

GAATCAATAC

GAAATCCAGG

GCACAACTGT

GTTCATTCCC

TTGGGAATTA

TACTGAGTAA

2521

TGGGAAGAAT

GTGCAAAAAA

TACCCGTGTC

TTATATAATT

TCCATTTGTG

TAGGGCTTAA

2581

AAGTGGACAA

CTTCAGGTTA

CGAAACCACG

AAAATGTCTA

AACGTTTGAT

CAAATTCGTA

2641

ACTCATGGAA

AATCACAGCA

ACAAATTAAG

GATGGATATA

TCCGGTACTT

ACAAATAAAA

2701

ACACTAAAGA

AGTATAAATT

TTGAACTGAA

ATTCTATAGA

AAATCTTTTT

TTTTTGGGTA

2761

AAATTATGCG

ATTGTGGCTC

ATAGTAACGT

AATCATACAG

TTCAACAAAC

ATCATTTTGT

2821

CAAAGGATTT

CTAATCCCAT

TCTTTTTCTA

CTTGGCTTTT

GAGTATATAT

ATATATATAC

2881

ATCCTTTGTT

CATCATCATG

TTCGATTGAA

TTTTTTTCTT

GGTTGTTATT

AGATTGTCTA

2941

CTTGAGTTTT

TCTTGGTGCA

AATTGTGGTG

TTTTTCTAAC

AAATTTTTCA

ACATTTAAAG

3001

TATTTCTGGA

ACTTTTCTAC

AAGGAACGAC

ATAGATCCAT

TAATGCGCCC

CTCTAAAACA

3061

ATACCCATAA

TGTCTTCTTA

AGTATGCATC

TGCTTATAAA

TGTTTCTGGA

TTTACTTTAG

3121

TTAGAATGTC

GACACGACAA

TGAACTATTG

CTGTGGATCA

CGTGCGATCA

CTGAATTATG

3181

AGTTTCTTTT

TATTTTTCTT

TTTGGGCTTT

TTTTTGCAAT

TTTTTTTTTC

CACCTTTGCT

3241

AATTGCGTAG

TCACATATAA

TGTATACAAT

TTTTGCAACT

CTGACACTAT

AATTGATCCA

3301

ATAATAAAGT

ATATAATAGA

TCATCAAGTA

TACGTAATCT

CCCCAAAAAA

AAAATTGCAT

3361

AAAATAAAAT

CTATAAACCT

TTTTTAGATT

TTTAGTTGTT

CCTTTTTTCT

TTCTATTCTT

3421

CAACTTCCAA

GTGTTGTCAC

TTTCTTCCTG

TTAACTATAT

TTAAAAGTAA

ATACCCTCCC

3481

CGGATCCATG

GTTGAAGATA

AAGATATAGA

CTTGAATATT

AATAATTTGA

AAATTCACGA

3541

TGCGCCAACA

CGAACACCAC

CGGTAAGCCT

GCCACCAGCT

TTACCAACAC

CACCAACTCC

3601

CTCAGGGATT

CTGCTCAACA

CTACAATGCA

AGCTAAATTG

ATGGCCTTTC

AACAACAAAG

3661

ATCGAAAGCA

GCAGCAGCAG

CAGCAGCAGC

AGCTTCAGTT

TCATCATCAT

CATCAGGTAC

3721

GCAGGCGTCA

TCGTCATCTA

TATCAGCTTC

AACATCGGAA

TCGAGTGTAT

CAACAATACC

3781

AGCAAATATT

AACCGAACTG

TATCGGGTAA

AAAGAAACCC

AAACCAAACT

TGAAATTAAG

3841

TGATTTACCA

TTGTCACGTA

ATAATAGTTT

ACACCGTTCT

AACACTAGTG

CTAGTGATTC

3901

AAGTGTGACT

ACGCCAGAAG

CAGATACCCC

TACCGGTAAG

ATTTCAAATG

AACCACAACC

3961

TCAAGGTTTG

TTTGCTAATT

ATTCTGATTA

TGTTGATATA

AAATCAGGTC

AATTAAATTT

4021

TGCTGGCAAA

GCATCATTAC

ATTCTAAAGG

GATTGATTTT

CTGTCTGGGT

CTTCATTTAG

4081

AGTTTCATTA

GATGAATTTG

AGTATTTAGA

AGAATTGGGC

CGTGGAAATT

ATGGGTCTGT

4141

ACTGAAAGTC

TTACATAAAC

CCACCGGTGT

ATTGATGGCA

ATGAAGGAAG

TTCGATTGGA

4201

GTTAGATGAG

AATAAGTTCA

CGCAAATACT

AATGGAGTTA

GATATTTTAC

ATAAATGTGA

4261

CTCGCCATAT

ATTGTTGATT

TTTATGGGGC

TTTTTTTGTT

GAAGGTGCAG

TTTACATGTG

4321

TATTGAGTAC

ATGGATGGAG

GTTCGTTGGA

TAGAATATTT

GGTAACGATG

TTGGTGTTAA

4381

AGATGAATAT

GAATTAGCCT

ATATCACTGA

GTCGGTTATA

CTTGGACTTA

AAGAATTGAA

4441

AGATAAACAT

AACATTATTC

ATCGTGATGT

CAAACCCACT

AATATTTTAG

TGAACACCCA

4501

GGGAAAAGTA

AAGTTGTGTG

ATTTTGGTGT

GTCTGGTAAT

TTAGTTGCCT

CATTAGCCAA

4561

AACAAATATT

GGTTGTCAAT

CATATATGGC

ACCGGAAAGG

ATCAACACTA

TGAGACCTGA

4621

TGATGCCACT

TATTCAGTTC

AATCAGATGT

TTGGTCATTG

GGGTTGACGA

TATTAGAATT

4681

AGCTGTTGGC

CATTATCCTT

ACCCTGCTGA

AACATATGAT

AATATTTTCT

CGCAATTAAG

4741

TGCTATTGTT

GATGGTGAAC

CACCAAAACT

TTACCCAAAG

GTATACTCCA

AGGAGGCACA

4801

AATATTTGTC

AAATCTTGTC

TTGCCAAAAA

CCCAGATTTA

AGACCATCTT

ACGCGGCATT

4861

ATTGAATAAT

CCATGGTTGA

TCAAAAACAG

AGGTAAAGAG

ACCAATCTTG

CTCAGACAGT

4921

AAAAGATAGA

GTAGAAGAAA

TTGCGAAATT

GGAGAAGAAC

AAGAGTGTCA

GTCGAAGCAA

4981

CAGCATGAAC

AAATCAGCAG

CCGCAGTGCC

TCCTCCGAGA

AATGTTGAAA

GTGTTCAATC

5041

ATTATTGAGA

AACAAAGTGA

AGGCTCCGGC

ATTACATAGA

GGTGGTTTAC

AAAAAGTGAA

5101

TAGAAGCTTT

CTTAATAATC

ATTGACTGCA

GGCATGCAAG

CTTCCGGATA

ATAGGAATTG

5161

ATTTGGATGG

TATAAACGGA

AACAAAAAAA

AGAGCTGGTA

CTACTTTCTT

TAAAATTATT

5221

TTATTATTTG

ATTTTATTTA

ATAGTATATA

TTATATTTTG

AACGTAGATT

ATTTTGTTGA

5281

AAGTTGCTGT

AGTGCCATTG

ATTCGTAACA

CTAATTCTGT

ATTAGTCATT

CCTCTTGTTT

5341

GATAGTATCC

AAAAAAACGG

CTATTTTTTT

GCAATCTTAT

TTCCTGCATA

TTATACAGAT

5401

AACATAATGA

AAGAAAAAAT

CTTTTTTTTT

GTTCTTCAAT

GATGATTTCA

ACCATTCTTT

5461

TAAACATTGA

TCAATTCCTG

AGCAACAACC

CCATACACAC

TGGTTTATAT

ATCGCCCCTT

5521

TTACAGTTGA

AGAAAGAAAT

AGAAATAGAA

ATAGCAAACA

AAAGATATGA

CAGTCAACAC

5581

TAAGACCTAT

AGTGAGAGAG

CAGAAACTCA

TGCCTCACCA

GTAGCACAAC

GATTATTTCG

5641

ATTAATGGAA

CTGAAGAAAA

CCAATTTATG

TGCATCAATT

GATGTTGATA

CCACTAAGGA

5701

ATTCCTTGAA

TTAATTGATA

AATTGGGTCC

TTATGTATGC

TTAATCAAGA

CTCATATTGA

5761

TATAATCAAT

GATTTTTCCT

ATGAATCCAC

TATTGAACCA

TTATTAGAAC

TTTCACGTAA

5821

ACATCAATTT

ATGATTTTTG

AAGATAGAAA

ATTTGCTGAT

ATTGGTAATA

CCGTGAAGAA

5881

ACAATATATT

GGTGGAGTTT

ATAAAATTAG

TAGTTGGGCA

GATATTACTA

ATGCTCATGG

5941

TGTCACTGGG

AATGGAGTAG

TTGAAGGATT

AAAACAGGGA

GCTAAAGAAA

CCACCACCAA

6001

CCAAGAGCCA

AGAGGGTTAT

TGATGTTAGC

TGAATTATCA

TCAGTGGGAT

CATTAGCATA

6061

TGGAGAATAT

TCTCAAAAAA

CTGTTGAAAT

TGCTAAATCC

GATAAGGAAT

TTGTTATTGG

6121

ATTTATTGCC

CAACGTGATA

TGGGTGGACA

AGAAGAAGGA

TTTGATTGGC

TTATTATGAC

6181

ACCTGGAGTT

GGATTAGATG

ATAAAGGTGA

TGGATTAGGA

CAACAATATA

GAACTGTTGA

6241

TGAAGTTGTT

AGCACTGGAA

CTGATATTAT

CATTGTTGGT

AGAGGATTGT

TTGGTAAAGG

6301

AAGAGATCCA

GATATTGAAG

GTAAAAGGTA

TAGAGATGCT

GGTTGGAATG

CTTATTTGAA

6361

AAAAACTGGC

CAATTATAAA

TGTGAAGGGG

GAGATTTTCA

CTTTATTAGA

TTTGTATATA

6421

TGTATAATAA

ATAAATAAAT

AAGTTAAATA

AATAATTAAA

TAAGGGTGGT

AATTATTACT

6481

ATTTACAATC

AAAGGTGGTC

CTTCTAAGCT

TGGCGTAATC

ATGGTCATAG

CTGTTTCCTG

6541

TGTGAAATTG

TTATCCGCTC

ACAATTCCAC

ACAACATACG

AGCCGGAAGC

ATAAAGTGTA

6601

AAGCCTGGGG

TGCCTAATGA

GTGAGCTAAC

TCACATTAAT

TGCGTTGCGC

TCACTGCCCG

6661

CTTTCCAGTC

GGGAAACCTG

TCGTGCCAGC

TGCATTAATG

AATCGGCCAA

CGCGCGGGGA

6721

GAGGCGGTTT

GCGTATTGGG

CGCTCTTCCG

CTTCCTCGCT

CACTGACTCG

CTGCGCTCGG

6781

TCGTTCGGCT

GCGGCGAGCG

GTATCAGCTC

ACTCAAAGGC

GGTAATACGG

TTATCCACAG

6841

AATCAGGGGA

TAACGCAGGA

AAGAACATGT

GAGCAAAAGG

CCAGCAAAAG

GCCAGGAACC

6901

GTAAAAAGGC

CGCGTTGCTG

GCGTTTTTCC

ATAGGCTCCG

CCCCCCTGAC

GAGCATCACA

6961

AAAATCGACG

CTCAAGTCAG

AGGTGGCGAA

ACCCGACAGG

ACTATAAAGA

TACCAGGCGT

7021

TTCCCCCTGG

AAGCTCCCTC

GTGCGCTCTC

CTGTTCCGAC

CCTGCCGCTT

ACCGGATACC

7081

TGTCCGCCTT

TCTCCCTTCG

GGAAGCGTGG

CGCTTTCTCA

TAGCTCACGC

TGTAGGTATC

7141

TCAGTTCGGT

GTAGGTCGTT

CGCTCCAAGC

TGGGCTGTGT

GCACGAACCC

CCCGTTCAGC

7201

CCGACCGCTG

CGCCTTATCC

GGTAACTATC

GTCTTGAGTC

CAACCCGGTA

AGACACGACT

7261

TATCGCCACT

GGCAGCAGCC

ACTGGTAACA

GGATTAGCAG

AGCGAGGTAT

GTAGGCGGTG

7321

CTACAGAGTT

CTTGAAGTGG

TGGCCTAACT

ACGGCTACAC

TAGAAGAACA

GTATTTGGTA

7381

TCTGCGCTCT

GCTGAAGCCA

GTTACCTTCG

GAAAAAGAGT

TGGTAGCTCT

TGATCCGGCA

7441

AACAAACCAC

CGCTGGTAGC

GGTGGTTTTT

TTGTTTGCAA

GCAGCAGATT

ACGCGCAGAA

7501

AAAAAGGATC

TCAAGAAGAT

CCTTTGATCT

TTTCTACGGG

GTCTGACGCT

CAGTGGAACG

7561

AAAACTCACG

TTAAGGGATT

TTGGTCATGA

GATTATCAAA

AAGGATCTTC

ACCTAGATCC

7621

TTTTAAATTA

AAAATGAAGT

TTTAAATCAA

TCTAAAGTAT

ATATGAGTAA

ACTTGGTCTG

7681

ACAGTTACCA

ATGCTTAATC

AGTGAGGCAC

CTATCTCAGC

GATCTGTCTA

TTTCGTTCAT

7741

CCATAGTTGC

CTGACTCCCC

GTCGTGTAGA

TAACTACGAT

ACGGGAGGGC

TTACCATCTG

7801

GCCCCAGTGC

TGCAATGATA

CCGCGAGACC

CACGCTCACC

GGCTCCAGAT

TTATCAGCAA

7861

TAAACCAGCC

AGCCGGAAGG

CCGAAGCGGC

AGAAGTGGTC

CTGCAACTTT

ATCCGCCT

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