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pLV3-CMV-Wdr11(mouse)-3×FLAG-CopGFP-Puro

产品货号:P56717

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pLV3-CMV-Wdr11(mouse)-3×FLAG-CopGFP-Puro
别称: NM_172255.3;2900055P10Rik; Brwd2; mKIAA1351
基因长: 3669bp
质粒宿主: 哺乳细胞,慢病毒
质粒用途: 蛋白表达
片段类型: ORF
片段物种: 小鼠
原核抗性: Amp
筛选标记: Puro
荧光标记: 绿色
启动子: CMV
复制子: pUC
拷贝数:
感受态: Stbl3
温度: 37度
背景载体:
正向引物: CMV-F
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

ACGCGTGTAG

TCTTATGCAA

TACTCTTGTA

GTCTTGCAAC

ATGGTAACGA

TGAGTTAGCA

61

ACATGCCTTA

CAAGGAGAGA

AAAAGCACCG

TGCATGCCGA

TTGGTGGAAG

TAAGGTGGTA

121

CGATCGTGCC

TTATTAGGAA

GGCAACAGAC

GGGTCTGACA

TGGATTGGAC

GAACCACTGA

181

ATTGCCGCAT

TGCAGAGATA

TTGTATTTAA

GTGCCTAGCT

CGATACAATA

AACGGGTCTC

241

TCTGGTTAGA

CCAGATCTGA

GCCTGGGAGC

TCTCTGGCTA

ACTAGGGAAC

CCACTGCTTA

301

AGCCTCAATA

AAGCTTGCCT

TGAGTGCTTC

AAGTAGTGTG

TGCCCGTCTG

TTGTGTGACT

361

CTGGTAACTA

GAGATCCCTC

AGACCCTTTT

AGTCAGTGTG

GAAAATCTCT

AGCAGTGGCG

421

CCCGAACAGG

GACCTGAAAG

CGAAAGGGAA

ACCAGAGCTC

TCTCGACGCA

GGACTCGGCT

481

TGCTGAAGCG

CGCACGGCAA

GAGGCGAGGG

GCGGCGACTG

GTGAGTACGC

CAAAAATTTT

541

GACTAGCGGA

GGCTAGAAGG

AGAGAGATGG

GTGCGAGAGC

GTCAGTATTA

AGCGGGGGAG

601

AATTAGATCG

CGATGGGAAA

AAATTCGGTT

AAGGCCAGGG

GGAAAGAAAA

AATATAAATT

661

AAAACATATA

GTATGGGCAA

GCAGGGAGCT

AGAACGATTC

GCAGTTAATC

CTGGCCTGTT

721

AGAAACATCA

GAAGGCTGTA

GACAAATACT

GGGACAGCTA

CAACCATCCC

TTCAGACAGG

781

ATCAGAAGAA

CTTAGATCAT

TATATAATAC

AGTAGCAACC

CTCTATTGTG

TGCATCAAAG

841

GATAGAGATA

AAAGACACCA

AGGAAGCTTT

AGACAAGATA

GAGGAAGAGC

AAAACAAAAG

901

TAAGACCACC

GCACAGCAAG

CGGCCACTGA

TCTTCAGACC

TGGAGGAGGA

GATATGAGGG

961

ACAATTGGAG

AAGTGAATTA

TATAAATATA

AAGTAGTAAA

AATTGAACCA

TTAGGAGTAG

1021

CACCCACCAA

GGCAAAGAGA

AGAGTGGTGC

AGAGAGAAAA

AAGAGCAGTG

GGAATAGGAG

1081

CTTTGTTCCT

TGGGTTCTTG

GGAGCAGCAG

GAAGCACTAT

GGGCGCAGCC

TCAATGACGC

1141

TGACGGTACA

GGCCAGACAA

TTATTGTCTG

GTATAGTGCA

GCAGCAGAAC

AATTTGCTGA

1201

GGGCTATTGA

GGCGCAACAG

CATCTGTTGC

AACTCACAGT

CTGGGGCATC

AAGCAGCTCC

1261

AGGCAAGAAT

CCTGGCTGTG

GAAAGATACC

TAAAGGATCA

ACAGCTCCTG

GGGATTTGGG

1321

GTTGCTCTGG

AAAACTCATT

TGCACCACTG

CTGTGCCTTG

GAATGCTAGT

TGGAGTAATA

1381

AATCTCTGGA

ACAGATTGGA

ATCACACGAC

CTGGATGGAG

TGGGACAGAG

AAATTAACAA

1441

TTACACAAGC

TTAATACACT

CCTTAATTGA

AGAATCGCAA

AACCAGCAAG

AAAAGAATGA

1501

ACAAGAATTA

TTGGAATTAG

ATAAATGGGC

AAGTTTGTGG

AATTGGTTTA

ACATAACAAA

1561

TTGGCTGTGG

TATATAAAAT

TATTCATAAT

GATAGTAGGA

GGCTTGGTAG

GTTTAAGAAT

1621

AGTTTTTGCT

GTACTTTCTA

TAGTGAATAG

AGTTAGGCAG

GGATATTCAC

CATTATCGTT

1681

TCAGACCCAC

CTCCCAACCC

CGAGGGGACC

CGACAGGCCC

GAAGGAATAG

AAGAAGAAGG

1741

TGGAGAGAGA

GACAGAGACA

GATCCATTCG

ATTAGTGAAC

GGATCTCGAC

GGTATCGGTT

1801

AACTTTTAAA

AGAAAAGGGG

GGATTGGGGG

GTACAGTGCA

GGGGAAAGAA

TAGTAGACAT

1861

AATAGCAACA

GACATACAAA

CTAAAGAATT

ACAAAAACAA

ATTACAAAAA

TTCAAAATTT

1921

TATCGATACT

AGTATTATGC

CCAGTACATG

ACCTTATGGG

ACTTTCCTAC

TTGGCAGTAC

1981

ATCTACGTAT

TAGTCATCGC

TATTACCATG

GTGATGCGGT

TTTGGCAGTA

CATCAATGGG

2041

CGTGGATAGC

GGTTTGACTC

ACGGGGATTT

CCAAGTCTCC

ACCCCATTGA

CGTCAATGGG

2101

AGTTTGTTTT

GGCACCAAAA

TCAACGGGAC

TTTCCAAAAT

GTCGTAACAA

CTCCGCCCCA

2161

TTGACGCAAA

TGGGCGGTAG

GCGTGTACGG

TGGGAGGTTT

ATATAAGCAG

AGCTCGTTTA

2221

GTGAACCGTC

AGATCGCCTG

GAGACGCCAT

CCACGCTGTT

TTGACCTCCA

TAGAAGATTC

2281

TAGAGCTAGC

CTGATACGAA

CTCGGAATTC

GCCACCATGT

TACCCTACAC

CGTAAACTTC

2341

AAGGTGTCAG

CGCGCACCCT

CACCGGGGCT

CTCAACGCGC

ACAACAAGGC

GGCGGTGGAC

2401

TGGGGCTGGC

AAGGTTTAAT

TGCATATGGA

TGCCATTCAC

TGGTGGTAGT

GATCGATTCC

2461

AATACTGCCC

AAACTCTACA

AGTTCTAGAA

AAACATAAAG

CTGATATTGT

TAAGGTCAGG

2521

TGGGCCAGGG

AGAACTATCA

CCATAACATC

GGCTCTCCAT

ACTGCCTGCG

CTTGGCTTCT

2581

GCGGATGTCA

CTGGGAAGAT

CATTGTCTGG

GATGTAGCAG

CCGGAGTAGC

CCAGTGTGAG

2641

ATCCAAGAGC

ACGTGAAGCC

CATCCAAGAT

GTGCAGTGGC

TGTGGAATCA

GGACGCTTCC

2701

CGAGACTTGC

TGCTCGCTAT

CCACCCGCCT

AATTACATCG

TGCTGTGGAA

TGCAGACACA

2761

GGCACCAAGC

TGTGGAAGAA

GAGCTACGCA

GATAACATCC

TTTCTTTTTC

TTTTGACCCT

2821

TTTGATCCTT

CACATTTAAC

TTTACTCACC

AGCGAGGGCA

TCGTTTTCAT

CTCAGACTTC

2881

TCTCCATCCA

AGCCTCCCTC

CGGCCCTGGG

AAGAAAGTGT

ACATCTCCAG

CCCACACTCC

2941

AGCCCAGCTC

ATAACAAGCT

GGCCGCAGCC

ACGGGAGCCA

AGAAGGCTCT

TAATAAAGTA

3001

AAGATTTTAA

TCACTCAGGA

GAAACCGAGT

GCTGATTTTG

TAGCTCTCAA

TGACTGCCTC

3061

CAGTTAGCCT

ATCTGCCTTC

AAAAAGGAAT

CACATGCTGT

TGCTCTACCC

TCGGGAGATT

3121

TTAATCCTTG

ACCTGGAAGT

GAATCAAACA

GTTGGTGTGA

TTGCAATCGA

GAGAACAGGC

3181

GTTCCCTTTC

TGCAGGTAAT

ACCATGCTCT

CAGCGAGATG

GCTTGTTTTG

CCTACATGAA

3241

AATGGCTGCA

TAACCTTACG

TGTCCGGAGG

TCTTATAACA

GCATCTGTAC

TACATCAAAT

3301

GATGAACCAG

ATCTAGATCC

TGTCCAGGAG

CTTACCTATG

ACTTGAGAAG

CCAGTGTGAT

3361

GCAATCAGGG

TGACGAAAAC

TGTCCGTCCC

TTCAGTATGG

TGTGCTGTCC

TGTTAATGAG

3421

AATGCAGCTG

CCCTCATAGT

AAGCGACGGC

AGAGTCATGA

TATGGGAACT

CAAGTCTGCT

3481

GTGTGCAGTC

GAAATGCACG

GAACAGTTCC

GGTGTGTCTC

CTTTATACTC

GCCAGTGTCC

3541

TTCTGTGGGA

TTCCTGGAGG

AGTGCTACAG

AATAAACTCC

CAGACCTTTC

CCTAGATAAT

3601

ATGATCGGGC

AGAGTGCAAT

TGCTGGGGAA

GAACACCCCA

AAGGCTCCAT

TCTGCAGGAA

3661

GTGCACCTCA

AGTTCCTGCT

GACGGGACTG

CTCTCAGGAC

TCCCTTCCCC

TCAGTTTGCC

3721

ATCCGAATGT

GCCCACCACT

GACCACAAAA

AACATTAAGA

TGTACCAGCC

ACTCCTTGCT

3781

GTTGGTACGA

GTAACGGTTC

TGTCCTGGTG

TACCACCTCA

CCAGTGGCCT

GCTGCACAAG

3841

GAGCTCAGTG

TCCACTCGTG

TGAAGTCAAG

GGTATTGAAT

GGACAAGCTT

GACCAGTTTC

3901

CTTTCTTTTG

CTGCTTCGAC

CCCAAACAAC

ATGGGGTTAG

TAAGAAATGA

ACTTCAGCTG

3961

GTTGATCTCC

CAACAGGGAG

GAGCACTGCT

TTCCGTGGTG

ACCGGGGCAA

TGACGAATCA

4021

CCCATTGAAA

TGATCAAAGT

GTCTCATCTG

AAACAGTATT

TGGCAGTTGT

TTTCAAAGAT

4081

AAGCCCCTGG

AATTATGGGA

TATTAGAACT

TGCACTCTTC

TTAGAGAAAT

GTCTAAAAGT

4141

TTCCCTGCTA

TAACTGCATT

GGAGTGGTCA

CCATCTCACA

ACCTGAAGAG

CCTGAGAAAG

4201

AAGCAGCTTG

CCACCCGGGA

GGCCATGGCC

CGCCAGACTG

TGGTCTCCGA

TGCAGAGCTG

4261

GGTGCTGTTG

AATCATCTGT

GATCAGTTTA

TTACAGGAGG

CAGAGAGTAA

GGCTGAACTC

4321

AGTCAGAACA

TCTCTGCTCG

GGAGCATTTT

GTGTTTACTG

ACAATGATGG

CCAAGTTTAT

4381

CATCTCACTG

TTGAAGGAAA

TTCTGTGAAA

GACAGTGCTC

GGATTCCACC

AGATGGTAGC

4441

ATGGGCAGTA

TTACCTGCAT

CGCTTGGAAG

GGGGATACAT

TAGTGCTTGG

AGATATGGAT

4501

GGAAATTTAA

ACTTCTGGGA

TTTGAAAGCC

AGAGTATCCA

GAGGAATACC

CACACATAGA

4561

AGTTGGGTGA

GGAAGATTCG

CTTTGCCCCC

GGCAAGGGAA

ACCAGAAGCT

AATAGCAATG

4621

TATAATGATG

GTGCTGAAGT

GTGGGATACT

AAAGAGGTTC

AGATGGTGAG

CAGTTTAAGA

4681

AGTGGAAGAA

ATGTAACCTT

CCGGATTTTG

GATGTGGACT

GGTGCACATC

AGATAAGGTG

4741

ATCTTGGCAT

CTGATGACGG

CTGCATCAGA

GTCCTGGAGA

TGTCAATGAA

GTCTACGTGT

4801

TTCAGAATGG

ATGAGCAGGA

GTTAGTAGAG

CCTGTGTGGT

GCCCGTACCT

CCTTGTTCCG

4861

AGGGCTGCCC

TTGCTTTGAA

AGCCTTCCTA

CTGCATCAGC

CTTGGAATGG

GCGATATTCG

4921

TTGGACATTT

CTCACATTGA

TTATCCAGAA

AATGAAGAAA

TAAAGACTCT

CCTTCAAGAA

4981

CAGTTGCATG

CATTGTCTAA

CGACATAAAG

AAACTCCTGC

TTGATCCCGA

CTTCTCTCTC

5041

TTGCAGAGGT

GCCTGCTCGT

GTCCAGGCTG

TACGGCGATG

AATCGGAGCT

GCACTTCTGG

5101

ACAGTGGCGG

CCCACTACCT

GCACAGCTTG

TCCCAGGCCA

AGTCTGGGGA

CACAGTGGTA

5161

ACCAAGGAAG

GTGCTCCTAA

GGACAGGCTG

AGCAACCCAT

TGGACATCTG

CTACGATGTG

5221

CTCTGTGAGA

ACACCTACTT

CCAGAAATTT

CAGCTAGAAA

GAGTTAATCT

ACAGGAAGTA

5281

AAACGGTCAA

CTTATGATCA

CACGAGAAAG

TGCACAGACC

AGCTCCTGCT

GCTAGGCCAG

5341

ACAGACAGAG

CTGTGCAGCT

GCTGTTGGAA

ACAAGTGCAG

ATAACCAGCA

CTACTACTGC

5401

GACTCTCTGA

AAGCTTGCTT

GGTCACCACT

GTCACCTCTT

CAGGCCCTTC

TCAGAGCACA

5461

ATTAAGCTGG

TAGCAACCAA

TATGATCGCC

AATGGCAAGC

TGGCAGAGGG

TGTTCAGTTA

5521

CTCTGCTTGA

TAGACAAGGC

TGCGGACGCC

TGTCGCTACC

TGCAGACGTA

TGGCGAGTGG

5581

AATCGGGCGG

CATGGCTTGC

AAAGGTTCGG

TTAAATTCTG

AAGAATGTGC

AGATGTTCTG

5641

AAGCGGTGGG

TTGACCACCT

TTGTTCTCCA

CAAGTCAACC

AGAAGTCCAA

GGCCCTCCTG

5701

GTCCTCCTCT

CTCTGGGTTG

CTTTGTCAGC

GTGGCAGAGA

CGCTTCACAG

CATGAGATAC

5761

TTCGATAGAG

CTGCCTTGTT

TGTGGAAGCC

TGTCTCAAGT

ACGGTGCATT

TGAAGTCAGT

5821

GAGGACACAG

AGAAGCTCAT

TGCTGCCATC

TACGCAGACT

ATGCCCGGAG

CCTGAAGAGC

5881

CTCGGCTTTA

GACAGGGAGC

AGTGCGTTTT

GCTTCAAAAG

CTGGAGCGGC

TGGCAGAGAT

5941

TTGTTGAATG

AGCTTGGGTC

CACCAAGGAA

GAGCTAACGG

AAAGTGGATC

CGACTACAAA

6001

GACCATGACG

GTGATTATAA

AGATCATGAC

ATCGACTACA

AGGATGACGA

TGACAAGTAG

6061

CGGCCGCGAA

GGATCTGCGA

TCGCTCCGGT

GCCCGTCAGT

GGGCAGAGCG

CACATCGCCC

6121

ACAGTCCCCG

AGAAGTTGGG

GGGAGGGGTC

GGCAATTGAA

CGGGTGCCTA

GAGAAGGTGG

6181

CGCGGGGTAA

ACTGGGAAAG

TGATGTCGTG

TACTGGCTCC

GCCTTTTTCC

CGAGGGTGGG

6241

GGAGAACCGT

ATATAAGTGC

AGTAGTCGCC

GTGAACGTTC

TTTTTCGCAA

CGGGTTTGCC

6301

GCCAGAACAC

AGCTGAAGCT

TCGAGGGGCT

CGCATCTCTC

CTTCACGCGC

CCGCCGCCCT

6361

ACCTGAGGCC

GCCATCCACG

CCGGTTGAGT

CGCGTTCTGC

CGCCTCCCGC

CTGTGGTGCC

6421

TCCTGAACTG

CGTCCGCCGT

CTAGGTAAGT

TTAAAGCTCA

GGTCGAGACC

GGGCCTTTGT

6481

CCGGCGCTCC

CTTGGAGCCT

ACCTAGACTC

AGCCGGCTCT

CCACGCTTTG

CCTGACCCTG

6541

CTTGCTCAAC

TCTACGTCTT

TGTTTCGTTT

TCTGTTCTGC

GCCGTTACAG

ATCCAAGCTG

6601

TGACCGGCGC

CTACGCTAGA

CGCCACCATG

GAGAGCGACG

AGAGCGGCCT

GCCCGCCATG

6661

GAGATCGAGT

GCCGCATCAC

CGGCACCCTG

AACGGCGTGG

AGTTCGAGCT

GGTGGGCGGC

6721

GGAGAGGGCA

CCCCCAAGCA

GGGCCGCATG

ACCAACAAGA

TGAAGAGCAC

CAAAGGCGCC

6781

CTGACCTTCA

GCCCCTACCT

GCTGAGCCAC

GTGATGGGCT

ACGGCTTCTA

CCACTTCGGC

6841

ACCTACCCCA

GCGGCTACGA

GAACCCCTTC

CTGCACGCCA

TCAACAACGG

CGGCTACACC

6901

AACACCCGCA

TCGAGAAGTA

CGAGGACGGC

GGCGTGCTGC

ACGTGAGCTT

CAGCTACCGC

6961

TACGAGGCCG

GCCGCGTGAT

CGGCGACTTC

AAGGTGGTGG

GCACCGGCTT

CCCCGAGGAC

7021

AGCGTGATCT

TCACCGACAA

GATCATCCGC

AGCAACGCCA

CCGTGGAGCA

CCTGCACCCC

7081

ATGGGCGATA

ACGTGCTGGT

GGGCAGCTTC

GCCCGCACCT

TCAGCCTGCG

CGACGGCGGC

7141

TACTACAGCT

TCGTGGTGGA

CAGCCACATG

CACTTCAAGA

GCGCCATCCA

CCCCAGCATC

7201

CTGCAGAACG

GGGGCCCCAT

GTTCGCCTTC

CGCCGCGTGG

AGGAGCTGCA

CAGCAACACC

7261

GAGCTGGGCA

TCGTGGAGTA

CCAGCACGCC

TTCAAGACCC

CCATCGCCTT

CGCCAGATCC

7321

CGCGCTCAGT

CGTCCAATTC

TGCCGTGGAC

GGCACCGCCG

GACCCGGCTC

CACCGGATCT

7381

CGCGAGGGCA

GAGGAAGTCT

TCTAACATGC

GGTGACGTGG

AGGAGAATCC

CGGCCCTATG

7441

ACCGAGTACA

AGCCCACGGT

GCGCCTCGCC

ACCCGCGACG

ACGTCCCCAG

GGCCGTACGC

7501

ACCCTCGCCG

CCGCGTTCGC

CGACTACCCC

GCCACGCGCC

ACACCGTCGA

TCCGGACCGC

7561

CACATCGAGC

GGGTCACCGA

GCTGCAAGAA

CTCTTCCTCA

CGCGCGTCGG

GCTCGACATC

7621

GGCAAGGTGT

GGGTCGCGGA

CGACGGCGCC

GCGGTGGCGG

TCTGGACCAC

GCCGGAGAGC

7681

GTCGAAGCGG

GGGCGGTGTT

CGCCGAGATC

GGCCCGCGCA

TGGCCGAGTT

GAGCGGTTCC

7741

CGGCTGGCCG

CGCAGCAACA

GATGGAAGGC

CTCCTGGCGC

CGCACCGGCC

CAAGGAGCCC

7801

GCGTGGTTCC

TGGCCACCGT

CGGCGTCTCG

CCCGACCACC

AGGGCAAGGG

TCTGGGCAGC

7861

GCCGTCGTGC

TCCCCGGAGT

GGAGGCGGCC

GAGCGCGCCG

GGGTGCCCGC

CTTCCTGGAG

7921

ACCTCCGCGC

CCCGCAACCT

CCCCTTCTAC

GAGCGGCTCG

GCTTCACCGT

CACCGCCGAC

7981

GTCGAGGTGC

CCGAAGGACC

GCGCACCTGG

TGCATGACCC

GCAAGCCCGG

TGCCTGAAAT

8041

CAACCTCTGG

ATTACAAAAT

TTGTGAAAGA

TTGACTGGTA

TTCTTAACTA

TGTTGCTCCT

8101

TTTACGCTAT

GTGGATACGC

TGCTTTAATG

CCTTTGTATC

ATGCTATTGC

TTCCCGTATG

8161

GCTTTCATTT

TCTCCTCCTT

GTATAAATCC

TGGTTGCTGT

CTCTTTATGA

GGAGTTGTGG

8221

CCCGTTGTCA

GGCAACGTGG

CGTGGTGTGC

ACTGTGTTTG

CTGACGCAAC

CCCCACTGGT

8281

TGGGGCATTG

CCACCACCTG

TCAGCTCCTT

TCCGGGACTT

TCGCTTTCCC

CCTCCCTATT

8341

GCCACGGCGG

AACTCATCGC

CGCCTGCCTT

GCCCGCTGCT

GGACAGGGGC

TCGGCTGTTG

8401

GGCACTGACA

ATTCCGTGGT

GTTGTCGGGG

AAATCATCGT

CCTTTCCTTG

GCTGCTCGCC

8461

TGTGTTGCCA

CCTGGATTCT

GCGCGGGACG

TCCTTCTGCT

ACGTCCCTTC

GGCCCTCAAT

8521

CCAGCGGACC

TTCCTTCCCG

CGGCCTGCTG

CCGGCTCTGC

GGCCTCTTCC

GCGTCTTCGC

8581

CTTCGCCCTC

AGACGAGTCG

GATCTCCCTT

TGGGCCGCCT

CCCCGCCTGG

TACCTTTAAG

8641

ACCAATGACT

TACAAGGCAG

CTGTAGATCT

TAGCCACTTT

TTAAAAGAAA

AGGGGGGACT

8701

GGAAGGGCTA

ATTCACTCCC

AACGAAGATA

AGATCTGCTT

TTTGCTTGTA

CTGGGTCTCT

8761

CTGGTTAGAC

CAGATCTGAG

CCTGGGAGCT

CTCTGGCTAA

CTAGGGAACC

CACTGCTTAA

8821

GCCTCAATAA

AGCTTGCCTT

GAGTGCTTCA

AGTAGTGTGT

GCCCGTCTGT

TGTGTGACTC

8881

TGGTAACTAG

AGATCCCTCA

GACCCTTTTA

GTCAGTGTGG

AAAATCTCTA

GCAGTAGTAG

8941

TTCATGTCAT

CTTATTATTC

AGTATTTATA

ACTTGCAAAG

AAATGAATAT

CAGAGAGTGA

9001

GAGGAACTTG

TTTATTGCAG

CTTATAATGG

TTACAAATAA

AGCAATAGCA

TCACAAATTT

9061

CACAAATAAA

GCATTTTTTT

CACTGCATTC

TAGTTGTGGT

TTGTCCAAAC

TCATCAATGT

9121

ATCTTATCAT

GTCTGGCTCT

AGCTATCCCG

CCCCTAACTC

CGCCCATCCC

GCCCCTAACT

9181

CCGCCCAGTT

CCGCCCATTC

TCCGCCCCAT

GGCTGACTAA

TTTTTTTTAT

TTATGCAGAG

9241

GCCGAGGCCG

CCTCGGCCTC

TGAGCTATTC

CAGAAGTAGT

GAGGAGGCTT

TTTTGGAGGC

9301

CTAGACTTTT

GCAGAGACCA

AATTCGTAAT

CATGTCATAG

CTGTTTCCTG

TGTGAAATTG

9361

TTATCCGCTC

ACAATTCCAC

ACAACATACG

AGCCGGAAGC

ATAAAGTGTA

AAGCCTGGGG

9421

TGCCTAATGA

GTGAGCTAAC

TCACATTAAT

TGCGTTGCGC

TCACTGCCCG

CTTTCCAGTC

9481

GGGAAACCTG

TCGTGCCAGC

TGCATTAATG

AATCGGCCAA

CGCGCGGGGA

GAGGCGGTTT

9541

GCGTATTGGG

CGCTCTTCCG

CTTCCTCGCT

CACTGACTCG

CTGCGCTCGG

TCGTTCGGCT

9601

GCGGCGAGCG

GTATCAGCTC

ACTCAAAGGC

GGTAATACGG

TTATCCACAG

AATCAGGGGA

9661

TAACGCAGGA

AAGAACATGT

GAGCAAAAGG

CCAGCAAAAG

GCCAGGAACC

GTAAAAAGGC

9721

CGCGTTGCTG

GCGTTTTTCC

ATAGGCTCCG

CCCCCCTGAC

GAGCATCACA

AAAATCGACG

9781

CTCAAGTCAG

AGGTGGCGAA

ACCCGACAGG

ACTATAAAGA

TACCAGGCGT

TTCCCCCTGG

9841

AAGCTCCCTC

GTGCGCTCTC

CTGTTCCGAC

CCTGCCGCTT

ACCGGATACC

TGTCCGCCTT

9901

TCTCCCTTCG

GGAAGCGTGG

CGCTTTCTCA

TAGCTCACGC

TGTAGGTATC

TCAGTTCGGT

9961

GTAGGTCGTT

CGCTCCAAGC

TGGGCTGTGT

GCACGAACCC

CCCGTTCAGC

CCGACCGCTG

10021

CGCCTTATCC

GGTAACTATC

GTCTTGAGTC

CAACCCGGTA

AGACACGACT

TATCGCCACT

10081

GGCAGCAGCC

ACTGGTAACA

GGATTAGCAG

AGCGAGGTAT

GTAGGCGGTG

CTACAGAGTT

10141

CTTGAAGTGG

TGGCCTAACT

ACGGCTACAC

TAGAAGGACA

GTATTTGGTA

TCTGCGCTCT

10201

GCTGAAGCCA

GTTACCTTCG

GAAAAAGAGT

TGGTAGCTCT

TGATCCGGCA

AACAAACCAC

10261

CGCTGGTAGC

GGTGGTTTTT

TTGTTTGCAA

GCAGCAGATT

ACGCGCAGAA

AAAAAGGATC

10321

TCAAGAAGAT

CCTTTGATCT

TTTCTACGGG

GTCTGACGCT

CAGTGGAACG

AAAACTCACG

10381

TTAAGGGATT

TTGGTCATGA

GATTATCAAA

AAGGATCTTC

ACCTAGATCC

TTTTAAATTA

10441

AAAATGAAGT

TTTAAATCAA

TCTAAAGTAT

ATATGAGTAA

ACTTGGTCTG

ACAGTTACCA

10501

ATGCTTAATC

AGTGAGGCAC

CTATCTCAGC

GATCTGTCTA

TTTCGTTCAT

CCATAGTTGC

10561

CTGACTCCCC

GTCGTGTAGA

TAACTACGAT

ACGGGAGGGC

TTACCATCTG

GCCCCAGTGC

10621

TGCAATGATA

CCGCGAGACC

CACGCTCACC

GGCTCCAGAT

TTATCAGCAA

TAAACCAGCC

10681

AGCCGGAAGG

GCCGAGCGCA

GAAGTGGTCC

TGCAACTTTA

TCCGCCTCCA

TCCAGTCTAT

10741

TAATTGTTGC

CGGGAAGCTA

GAGTAAGTAG

TTCGCCAGTT

AATAGTTTGC

GCAACGTTGT

10801

TGCCATTGCT

ACAGGCATCG

TGGTGTCACG

CTCGTCGTTT

GGTATGGCTT

CATTCAGCTC

10861

CGGTTCCCAA

CGATCAAGGC

GAGTTACATG

ATCCCCCATG

TTGTGCAAAA

AAGCGGTTAG

10921

CTCCTTCGGT

CCTCCGATCG

TTGTCAGAAG

TAAGTTGGCC

GCAGTGTTAT

CACTCATGGT

10981

TATGGCAGCA

CTGCATAATT

CTCTTACTGT

CATGCCATCC

GTAAGATGCT

TTTCTGTGAC

11041

TGGTGAGTAC

TCAACCAAGT

CATTCTGAGA

ATAGTGTATG

CGGCGACCGA

GTTGCTCTTG

11101

CCCGGCGTCA

ATACGGGATA

ATACCGCGCC

ACATAGCAGA

ACTTTAAAAG

TGCTCATCAT

11161

TGGAAAACGT

TCTTCGGGGC

GAAAACTCTC

AAGGATCTTA

CCGCTGTTGA

GATCCAGTTC

11221

GATGTAACCC

ACTCGTGCAC

CCAACTGATC

TTCAGCATCT

TTTACTTTCA

CCAGCGTTTC

11281

TGGGTGAGCA

AAAACAGGAA

GGCAAAATGC

CGCAAAAAAG

GGAATAAGGG

CGACACGGAA

11341

ATGTTGAATA

CTCATACTCT

TCCTTTTTCA

ATATTATTGA

AGCATTTATC

AGGGTTATTG

11401

TCTCATGAGC

GGATACATAT

TTGAATGTAT

TTAGAAAAAT

AAACAAATAG

GGGTTCCGCG

11461

CACATTTCCC

CGAAAAGTGC

CACCTGACGT

CTAAGAAACC

ATTATTATCA

TGACATTAAC

11521

CTATAAAAAT

AGGCGTATCA

CGAGGCCCTT

TCGTCTCGCG

CGTTTCGGTG

ATGACGGTGA

11581

AAACCTCTGA

CACATGCAGC

TCCCGGAGAC

GGTCACAGCT

TGTCTGTAAG

CGGATGCCGG

11641

GAGCAGACAA

GCCCGTCAGG

GCGCGTCAGC

GGGTGTTGGC

GGGTGTCGGG

GCTGGCTTAA

11701

CTATGCGGCA

TCAGAGCAGA

TTGTACTGAG

AGTGCACCAT

ATGCGGTGTG

AAATACCGCA

11761

CAGATGCGTA

AGGAGAAAAT

ACCGCATCAG

GCGCCATTCG

CCATTCAGGC

TGCGCAACTG

11821

TTGGGAAGGG

CGATCGGTGC

GGGCCTCTTC

GCTATTACGC

CAGCTGGCGA

AAGGGGGATG

11881

TGCTGCAAGG

CGATTAAGTT

GGGTAACGCC

AGGGTTTTCC

CAGTCACGAC

GTTGTAAAAC

11941

GACGGCCAGT

GCCAAGCTG

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