名称: | pCMV-Cubn(rat)(1-928aa)-3×FLAG-Neo |
---|---|
别称: | NM_053332.4;GP280; IFCR; MGA1; |
基因长: | 2784bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 大鼠 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTTAGGCGTT
TTGCGCTGCT
TCGCGATGTA
CGGGCCAGAT
ATACGCGTTG
ACATTGATTA
TTGACTAGTT
ATTAATAGTA
ATCAATTACG
GGGTCATTAG
TTCATAGCCC
ATATATGGAG
TTCCGCGTTA
CATAACTTAC
GGTAAATGGC
CCGCCTGGCT
GACCGCCCAA
CGACCCCCGC
CCATTGACGT
CAATAATGAC
GTATGTTCCC
ATAGTAACGC
CAATAGGGAC
TTTCCATTGA
CGTCAATGGG
TGGAGTATTT
ACGGTAAACT
GCCCACTTGG
CAGTACATCA
AGTGTATCAT
ATGCCAAGTA
CGCCCCCTAT
TGACGTCAAT
GACGGTAAAT
GGCCCGCCTG
GCATTATGCC
CAGTACATGA
CCTTATGGGA
CTTTCCTACT
TGGCAGTACA
TCTACGTATT
AGTCATCGCT
ATTACCATGG
TGATGCGGTT
TTGGCAGTAC
ATCAATGGGC
GTGGATAGCG
GTTTGACTCA
CGGGGATTTC
CAAGTCTCCA
CCCCATTGAC
GTCAATGGGA
GTTTGTTTTG
GCACCAAAAT
CAACGGGACT
TTCCAAAATG
TCGTAACAAC
TCCGCCCCAT
TGACGCAAAT
GGGCGGTAGG
CGTGTACGGT
GGGAGGTCTA
TATAAGCAGA
GCTCTCTGGC
TAACTAGAGA
ACCCACTGCT
TACTGGCTTA
TCGAAATTAA
TACGACTCAC
TATAGGGAGA
CCCAAGCTGG
CTAGCGTTTA
AACTTAAGCT
TGGTACCGAG
CTCGAATTCG
CCACCATGTC
CTCGCAGTTT
CTCTGGGGTT
TTGTTACTTT
GTTGATGATA
GCTGAATTAG
ATGGCAAAAC
TGGAAAGCCG
GAGCAGAGGG
GACAGAAAAG
GATCGCTGAC
CTGCACCAGC
CTCGGATGAC
TACGGAGGAG
GGAAACTTAG
TATTTCTTAC
AAGCTCTACC
CAAAACATTG
AATTTAGAAC
TGGATCCCTG
GGGAAAATCA
AATTAAATGA
TGAAGACCTT
GGCGAATGTT
TACACCAGAT
CCAGAGAAAC
AAAGATGATA
TTATAGATTT
AAGAAAGAAT
ACAACTGGCC
TCCCTCAAAA
TATACTTAGT
CAAGTCCACC
AACTTAACTC
GAAGCTCGTG
GATCTGGAGA
GAGATTTTCA
AAACTTACAG
CAGAATGTGG
AGAGAAAAGT
TTGCAGTAGC
AATCCCTGCC
TTAATGGTGG
CACCTGCGTC
AACCTGCACG
ACTCCTTTGT
GTGTATCTGC
CCTTCTCAGT
GGAAGGGTCT
CTTCTGCTCA
GAGGATGTCA
ACGAGTGTGT
AGTTTACTCA
GGAACACCCT
TTGGCTGCCA
GAGTGGATCC
ACCTGTGTGA
ACACAGTGGG
GAGTTTCAGA
TGTGACTGCA
CGCCGGATAC
GTACGGACCT
CAGTGTGCAT
CCAAATATAA
TGACTGTGAA
CAGGGCTCTA
AACAGCTCTG
CAAGCACGGC
ATCTGTGAGG
ATTTACAGCG
AGTTCACCAT
GGACAGCCGA
ATTTTCACTG
CATCTGTGAT
GCTGGGTGGA
CAACACCGCC
AAATGGAATC
TCCTGCACAG
AGGACAAAGA
CGAATGCAGC
CTCCAGCCTT
CACCTTGTTC
GGAGCATGCG
CAGTGTTTCA
ATACACAAGG
CTCCTTCTAC
TGTGGGGCCT
GCCCCAAAGG
TTGGCAGGGA
AATGGTTATG
AATGCCAGGA
TATCAATGAA
TGCGAGATCA
ACAATGGAGG
CTGTTCCCAG
GCGCCACTGG
TCCCATGCCT
GAACACACCT
GGATCATTCT
CCTGTGGGAA
CTGTCCAGCA
GGTTTCAGTG
GTGACGGGAG
AGTGTGCACT
CCCGTGGACA
TCTGTTCGAT
CCACAATGGA
GGCTGCCATC
CAGAGGCAAC
CTGCTCCTCA
TCTCCTGTTC
TAGGGTCCTT
CTTGCCTGTC
TGCACCTGTC
CTCCGGGATA
TACAGGAAAT
GGTTACGGGT
CTAATGGATG
TGTACGCCTC
AGTAATATTT
GCTCAAGGCA
CCCGTGTGTA
AATGGACAGT
GCATAGAAAC
AGTATCAAGT
TATTTCTGTA
AGTGTGACTC
AGGCTGGTCT
GGACAGAACT
GTACAGAAAA
CATTAATGAC
TGTTCGAGCA
ACCCCTGTTT
GAATGGGGGC
ACCTGTATTG
ATGGCATCAA
TGGCTTCACC
TGCGACTGCA
CAAGCTCTTG
GACTGGCTAT
TACTGTCAGA
CTCCCCAGGC
AGCTTGCGGA
GGGATCCTCT
CAGGGACACA
AGGAACCTTT
GCCTACCACA
GCCCAAATGA
TACCTATATT
CACAATGTGA
ACTGTTTCTG
GATTGTCAGA
ACTGATGAGG
AAAAGGTTCT
GCATGTCACC
TTCACGTTTT
TTGATTTAGA
ATCAGCAAGC
AATTGTCCCC
GGGAGTACCT
TCAGATTCAT
GATGGAGACT
CCTCAGCGGA
TTTCCCACTT
GGTAGATACT
GTGGCTCCAG
GCCCCCCCAG
GGGATCCACA
GCAGTGCCAA
TGCTCTCTAT
TTCCATCTCT
ACTCCGAGTA
CATAAGGAGT
GGGAGAGGCT
TTACAGCAAG
GTGGGAGGCG
AAGCTGCCAG
AGTGTGGCGG
CATCCTGACT
GATAATTACG
GTTCTATTAC
GTCTCCGGGG
TACCCTGGAA
ACTACCCCCC
AGGAAGAGAT
TGTGTCTGGC
AGGTTTTAGT
CAATCCTAAC
TCCTTGATAA
CATTTACCTT
TGGAACCTTG
AGCCTGGAGA
GCCACAATGA
CTGCAGCAAA
GACTATTTGG
AGATTCGAGA
CGGTCCTTTC
CACCAAGACC
CTGTTCTTGG
GAAATTCTGC
ACTTCCTTGT
CTACCCCACC
CCTCAAGACT
ACCGGTCCTG
CAGCAAGAAT
TCATTTCCAT
TCTGACTCTG
AGACCAGTGA
CAAAGGCTTC
CACATCACCT
ATCTAACCAC
ACAGTCGGAT
CTGGACTGTG
GTGGGAACTA
CACAGACACG
GATGGCGAGC
TCCTCCTTCC
TCCTTTGTCT
GGTCCTTTCA
GTCACAGCAG
ACAGTGTGTC
TATCTCATCA
CCCAAGCCCA
GGGAGAACAA
ATAGTTATCA
ACTTCACCCA
TGTGGAGCTG
GAGAGCCAGA
TGGGCTGTTC
TCACACTTAC
ATCGAGGTTG
GAGACCATGA
CAGCTTACTT
CGAAAGATCT
GTGGCAATGA
AACCTTGTTC
CCCATTAGAT
CAGTTTCTAA
TAAAGTCTGG
ATCAGATTGA
GAATAGACGC
TTTAGTCCAG
AAGGCTAGTT
TCAGAGCTGA
CTACCAAGTT
GCTTGTGGGG
GTATGTTAAG
AGGAGAAGGA
TTCTTTCGCT
CACCTTTCTA
TCCTAACGCA
TATCCTGGAC
GAAGAACCTG
TAGGTGGACC
ATCTCCCAAC
CCCAAAGACA
AGTTGTCCTT
CTTAACTTCA
CTGACTTTCA
GATCGGAAGT
TCTGCCTCCT
GTGATACAGA
TTATATTGAG
ATTGGTCCCA
GCTCTGTCTT
GGGATCTCCT
GGAAATGAGA
AGTTTTGTAG
CTCAAACATA
CCGTCATTTA
TAACATCTGT
ATACAATATT
CTTTATGTTA
CATTTGTGAA
AAGTTCTTCC
ATGGAAAATC
GTGGCTTCAC
GGCTAAGTTC
AGCAGTGACG
GATCCGACTA
CAAAGACCAT
GACGGTGATT
ATAAAGATCA
TGACATCGAC
TACAAGGATG
ACGATGACAA
GTAGTGAGGG
CCCGATATCT
CGACAATCAA
CCTCTGGATT
ACAAAATTTG
TGAAAGATTG
ACTGGTATTC
TTAACTATGT
TGCTCCTTTT
ACGCTATGTG
GATACGCTGC
TTTAATGCCT
TTGTATCATG
CTATTGCTTC
CCGTATGGCT
TTCATTTTCT
CCTCCTTGTA
TAAATCCTGG
TTGCTGTCTC
TTTATGAGGA
GTTGTGGCCC
GTTGTCAGGC
AACGTGGCGT
GGTGTGCACT
GTGTTTGCTG
ACGCAACCCC
CACTGGTTGG
GGCATTGCCA
CCACCTGTCA
GCTCCTTTCC
GGGACTTTCG
CTTTCCCCCT
CCCTATTGCC
ACGGCGGAAC
TCATCGCCGC
CTGCCTTGCC
CGCTGCTGGA
CAGGGGCTCG
GCTGTTGGGC
ACTGACAATT
CCGTGGTGTT
GTCGGGGAAG
CTGACGTCCT
TTCCATGGCT
GCTCGCCTGT
GTTGCCACCT
GGATTCTGCG
CGGGACGTCC
TTCTGCTACG
TCCCTTCGGC
CCTCAATCCA
GCGGACCTTC
CTTCCCGCGG
CCTGCTGCCG
GCTCTGCGGC
CTCTTCCGCG
TCTTCGCCTT
CGCCCTCAGA
CGAGTCGGAT
CTCCCTTTGG
GCCGCCTCCC
CGCCTGGAAA
CGGGGGAGGC
TAACTGAAAC
ACGGAAGGAG
ACAATACCGG
AAGGAACCCG
CGCTATGACG
GCAATAAAAA
GACAGAATAA
AACGCACGGG
TGTTGGGTCG
TTTGTTCATA
AACGCGGGGT
TCGGTCCCAG
GGCTGGCACT
CTGTCGATAC
CCCACCGAGA
CCCCATTGGG
GCCAATACGC
CCGCGTTTCT
TCCTTTTCCC
CACCCCACCC
CCCAAGTTCG
GGTGAAGGCC
CAGGGCTCGC
AGCCAACGTC
GGGGCGGCAG
GCCCTGCCAT
AGCAGATCTG
CGCAGCTGGG
GCTCTAGGGG
GTATCCCCAC
GCGCCCTGTA
GCGGCGCATT
AAGCGCGGCG
GGTGTGGTGG
TTACGCGCAG
CGTGACCGCT
ACACTTGCCA
GCGCCCTAGC
GCCCGCTCCT
TTCGCTTTCT
TCCCTTCCTT
TCTCGCCACG
TTCGCCGGCT
TTCCCCGTCA
AGCTCTAAAT
CGGGGCATCC
CTTTAGGGTT
CCGATTTAGT
GCTTTACGGC
ACCTCGACCC
CAAAAAACTT
GATTAGGGTG
ATGGTTCACG
TAGTGGGCCA
TCGCCCTGAT
AGACGGTTTT
TCGCCCTTTG
ACGTTGGAGT
CCACGTTCTT
TAATAGTGGA
CTCTTGTTCC
AAACTGGAAC
AACACTCAAC
CCTATCTCGG
TCTATTCTTT
TGATTTATAA
GGGATTTTGG
GGATTTCGGC
CTATTGGTTA
AAAAATGAGC
TGATTTAACA
AAAATTTAAC
GCGAATTAAT
TCTGTGGAAT
GTGTGTCAGT
TAGGGTGTGG
AAAGTCCCCA
GGCTCCCCAG
CAGGCAGAAG
TATGCAAAGC
ATGCATCTCA
ATTAGTCAGC
AACCAGGTGT
GGAAAGTCCC
CAGGCTCCCC
AGCAGGCAGA
AGTATGCAAA
GCATGCATCT
CAATTAGTCA
GCAACCATAG
TCCCGCCCCT
AACTCCGCCC
ATCCCGCCCC
TAACTCCGCC
CAGTTCCGCC
CATTCTCCGC
CCCATGGCTG
ACTAATTTTT
TTTATTTATG
CAGAGGCCGA
GGCCGCCTCT
GCCTCTGAGC
TATTCCAGAA
GTAGTGAGGA
GGCTTTTTTG
GAGGCCTAGG
CTTTTGCAAA
AAGCTCCCGG
GAGCTTGTAT
ATCCATTTTC
GGATCTGATC
AAGAGACAGG
ATGAGGATCG
TTTCGCATGA
TTGAACAAGA
TGGATTGCAC
GCAGGTTCTC
CGGCCGCTTG
GGTGGAGAGG
CTATTCGGCT
ATGACTGGGC
ACAACAGACA
ATCGGCTGCT
CTGATGCCGC
CGTGTTCCGG
CTGTCAGCGC
AGGGGCGCCC
GGTTCTTTTT
GTCAAGACCG
ACCTGTCCGG
TGCCCTGAAT
GAACTGCAGG
ACGAGGCAGC
GCGGCTATCG
TGGCTGGCCA
CGACGGGCGT
TCCTTGCGCA
GCTGTGCTCG
ACGTTGTCAC
TGAAGCGGGA
AGGGACTGGC
TGCTATTGGG
CGAAGTGCCG
GGGCAGGATC
TCCTGTCATC
TCACCTTGCT
CCTGCCGAGA
AAGTATCCAT
CATGGCTGAT
GCAATGCGGC
GGCTGCATAC
GCTTGATCCG
GCTACCTGCC
CATTCGACCA
CCAAGCGAAA
CATCGCATCG
AGCGAGCACG
TACTCGGATG
GAAGCCGGTC
TTGTCGATCA
GGATGATCTG
GACGAAGAGC
ATCAGGGGCT
CGCGCCAGCC
GAACTGTTCG
CCAGGCTCAA
GGCGCGCATG
CCCGACGGCG
AGGATCTCGT
CGTGACCCAT
GGCGATGCCT
GCTTGCCGAA
TATCATGGTG
GAAAATGGCC
GCTTTTCTGG
ATTCATCGAC
TGTGGCCGGC
TGGGTGTGGC
GGACCGCTAT
CAGGACATAG
CGTTGGCTAC
CCGTGATATT
GCTGAAGAGC
TTGGCGGCGA
ATGGGCTGAC
CGCTTCCTCG
TGCTTTACGG
TATCGCCGCT
CCCGATTCGC
AGCGCATCGC
CTTCTATCGC
CTTCTTGACG
AGTTCTTCTG
AGCGGGACTC
TGGGGTTCGC
GAAATGACCG
ACCAAGCGAC
GCCCAACCTG
CCATCACGAG
ATTTCGATTC
CACCGCCGCC
TTCTATGAAA
GGTTGGGCTT
CGGAATCGTT
TTCCGGGACG
CCGGCTGGAT
GATCCTCCAG
CGCGGGGATC
TCATGCTGGA
GTTCTTCGCC
CACCCCAACT
TGTTTATTGC
AGCTTATAAT
GGTTACAAAT
AAAGCAATAG
CATCACAAAT
TTCACAAATA
AAGCATTTTT
TTCACTGCAT
TCTAGTTGTG
GTTTGTCCAA
ACTCATCAAT
GTATCTTATC
ATGTCTGTAT
ACCGTCGACC
TCTAGCTAGA
GCTTGGCGTA
ATCATGGTCA
TAGCTGTTTC
CTGTGTGAAA
TTGTTATCCG
CTCACAATTC
CACACAACAT
ACGAGCCGGA
AGCATAAAGT
GTAAAGCCTG
GGGTGCCTAA
TGAGTGAGCT
AACTCACATT
AATTGCGTTG
CGCTCACTGC
CCGCTTTCCA
GTCGGGAAAC
CTGTCGTGCC
AGCTGCATTA
ATGAATCGGC
CAACGCGCGG
GGAGAGGCGG
TTTGCGTATT
GGGCGCTCTT
CCGCTTCCTC
GCTCACTGAC
TCGCTGCGCT
CGGTCGTTCG
GCTGCGGCGA
GCGGTATCAG
CTCACTCAAA
GGCGGTAATA
CGGTTATCCA
CAGAATCAGG
GGATAACGCA
GGAAAGAACA
TGTGAGCAAA
AGGCCAGCAA
AAGGCCAGGA
ACCGTAAAAA
GGCCGCGTTG
CTGGCGTTTT
TCCATAGGCT
CCGCCCCCCT
GACGAGCATC
ACAAAAATCG
ACGCTCAAGT
CAGAGGTGGC
GAAACCCGAC
AGGACTATAA
AGATACCAGG
CGTTTCCCCC
TGGAAGCTCC
CTCGTGCGCT
CTCCTGTTCC
GACCCTGCCG
CTTACCGGAT
ACCTGTCCGC
CTTTCTCCCT
TCGGGAAGCG
TGGCGCTTTC
TCAATGCTCA
CGCTGTAGGT
ATCTCAGTTC
GGTGTAGGTC
GTTCGCTCCA
AGCTGGGCTG
TGTGCACGAA
CCCCCCGTTC
AGCCCGACCG
CTGCGCCTTA
TCCGGTAACT
ATCGTCTTGA
GTCCAACCCG
GTAAGACACG
ACTTATCGCC
ACTGGCAGCA
GCCACTGGTA
ACAGGATTAG
CAGAGCGAGG
TATGTAGGCG
GTGCTACAGA
GTTCTTGAAG
TGGTGGCCTA
ACTACGGCTA
CACTAGAAGG
ACAGTATTTG
GTATCTGCGC
TCTGCTGAAG
CCAGTTACCT
TCGGAAAAAG
AGTTGGTAGC
TCTTGATCCG
GCAAACAAAC
CACCGCTGGT
AGCGGTGGTT
TTTTTGTTTG
CAAGCAGCAG
ATTACGCGCA
GAAAAAAAGG
ATCTCAAGAA
GATCCTTTGA
TCTTTTCTAC
GGGGTCTGAC
GCTCAGTGGA
ACGAAAACTC
ACGTTAAGGG
ATTTTGGTCA
TGAGATTATC
AAAAAGGATC
TTCACCTAGA
TCCTTTTAAA
TTAAAAATGA
AGTTTTAAAT
CAATCTAAAG
TATATATGAG
TAAACTTGGT
CTGACAGTTA
CCAATGCTTA
ATCAGTGAGG
CACCTATCTC
AGCGATCTGT
CTATTTCGTT
CATCCATAGT
TGCCTGACTC
CCCGTCGTGT
AGATAACTAC
GATACGGGAG
GGCTTACCAT
CTGGCCCCAG
TGCTGCAATG
ATACCGCGAG
ACCCACGCTC
ACCGGCTCCA
GATTTATCAG
CAATAAACCA
GCCAGCCGGA
AGGGCCGAGC
GCAGAAGTGG
TCCTGCAACT
TTATCCGCCT
CCATCCAGTC
TATTAATTGT
TGCCGGGAAG
CTAGAGTAAG
TAGTTCGCCA
GTTAATAGTT
TGCGCAACGT
TGTTGCCATT
GCTACAGGCA
TCGTGGTGTC
ACGCTCGTCG
TTTGGTATGG
CTTCATTCAG
CTCCGGTTCC
CAACGATCAA
GGCGAGTTAC
ATGATCCCCC
ATGTTGTGCA
AAAAAGCGGT
TAGCTCCTTC
GGTCCTCCGA
TCGTTGTCAG
AAGTAAGTTG
GCCGCAGTGT
TATCACTCAT
GGTTATGGCA
GCACTGCATA
ATTCTCTTAC
TGTCATGCCA
TCCGTAAGAT
GCTTTTCTGT
GACTGGTGAG
TACTCAACCA
AGTCATTCTG
AGAATAGTGT
ATGCGGCGAC
CGAGTTGCTC
TTGCCCGGCG
TCAATACGGG
ATAATACCGC
GCCACATAGC
AGAACTTTAA
AAGTGCTCAT
CATTGGAAAA
CGTTCTTCGG
GGCGAAAACT
CTCAAGGATC
TTACCGCTGT
TGAGATCCAG
TTCGATGTAA
CCCACTCGTG
CACCCAACTG
ATCTTCAGCA
TCTTTTACTT
TCACCAGCGT
TTCTGGGTGA
GCAAAAACAG
GAAGGCAAAA
TGCCGCAAAA
AAGGGAATAA
GGGCGACACG
GAAATGTTGA
ATACTCATAC
TCTTCCTTTT
TCAATATTAT
TGAAGCATTT
ATCAGGGTTA
TTGTCTCATG
AGCGGATACA
TATTTGAATG
TATTTAGAAA
AATAAACAAA
TAGGGGTTCC
GCGCACATTT
CCCCGAAAAG
TGCCACCTGA
CGTCGACGGA
TCGGGAGATC
TCCCGATCCC
CTATGGTCGA
CTCTCAGTAC
AATCTGCTCT
GATGCCGCAT
AGTTAAGCCA
GTATCTGCTC
CCTGCTTGTG
TGTTGGAGGT
CGCTGAGTAG
TGCGCGAGCA
AAATTTAAGC
TACAACAAGG
CAAGGCTTGA
CCGACAATTG
CATGAAGAAT
CTGCTTAGG