名称: | pCasKP-apr-Hyg |
---|---|
别称: | |
基因长: | |
质粒宿主: | 大肠杆菌 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR,Red |
片段物种: | |
原核抗性: | Hyg |
筛选标记: | |
荧光标记: | |
启动子: | araBAD |
复制子: | pSC101 |
拷贝数: | 低 |
感受态: | DH5a |
温度: | 30度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TCAGATCCTT
CCGTATTTAG
CCAGTATGTT
CTCTAGTGTG
GTTCGTTGTT
TTTGCGTGAG
CCATGAGAAC
GAACCATTGA
GATCATACTT
ACTTTGCATG
TCACTCAAAA
ATTTTGCCTC
AAAACTGGTG
AGCTGAATTT
TTGCAGTTAA
AGCATCGTGT
AGTGTTTTTC
TTAGTCCGTT
ACGTAGGTAG
GAATCTGATG
TAATGGTTGT
TGGTATTTTG
TCACCATTCA
TTTTTATCTG
GTTGTTCTCA
AGTTCGGTTA
CGAGATCCAT
TTGTCTATCT
AGTTCAACTT
GGAAAATCAA
CGTATCAGTC
GGGCGGCCTC
GCTTATCAAC
CACCAATTTC
ATATTGCTGT
AAGTGTTTAA
ATCTTTACTT
ATTGGTTTCA
AAACCCATTG
GTTAAGCCTT
TTAAACTCAT
GGTAGTTATT
TTCAAGCATT
AACATGAACT
TAAATTCATC
AAGGCTAATC
TCTATATTTG
CCTTGTGAGT
TTTCTTTTGT
GTTAGTTCTT
TTAATAACCA
CTCATAAATC
CTCATAGAGT
ATTTGTTTTC
AAAAGACTTA
ACATGTTCCA
GATTATATTT
TATGAATTTT
TTTAACTGGA
AAAGATAAGG
CAATATCTCT
TCACTAAAAA
CTAATTCTAA
TTTTTCGCTT
GAGAACTTGG
CATAGTTTGT
CCACTGGAAA
ATCTCAAAGC
CTTTAACCAA
AGGATTCCTG
ATTTCCACAG
TTCTCGTCAT
CAGCTCTCTG
GTTGCTTTAG
CTAATACACC
ATAAGCATTT
TCCCTACTGA
TGTTCATCAT
CTGAGCGTAT
TGGTTATAAG
TGAACGATAC
CGTCCGTTCT
TTCCTTGTAG
GGTTTTCAAT
CGTGGGGTTG
AGTAGTGCCA
CACAGCATAA
AATTAGCTTG
GTTTCATGCT
CCGTTAAGTC
ATAGCGACTA
ATCGCTAGTT
CATTTGCTTT
GAAAACAACT
AATTCAGACA
TACATCTCAA
TTGGTCTAGG
TGATTTTAAT
CACTATACCA
ATTGAGATGG
GCTAGTCAAT
GATAATTACT
AGTCCTTTTC
CTTTGAGTTG
TGGGTATCTG
TAAATTCTGC
TAGACCTTTG
CTGGAAAACT
TGTAAATTCT
GCTAGACCCT
CTGTAAATTC
CGCTAGACCT
TTGTGTGTTT
TTTTTGTTTA
TATTCAAGTG
GTTATAATTT
ATAGAATAAA
GAAAGAATAA
AAAAAGATAA
AAAGAATAGA
TCCCAGCCCT
GTGTATAACT
CACTACTTTA
GTCAGTTCCG
CAGTATTACA
AAAGGATGTC
GCAAACGCTG
TTTGCTCCTC
TACAAAACAG
ACCTTAAAAC
CCTAAAGGCT
TAAGTAGCAC
CCTCGCAAGC
TCGGTTGCGG
CCGCAATCGG
GCAAATCGCT
GAATATTCCT
TTTGTCTCCG
ACCATCAGGC
ACCTGAGTCG
CTGTCTTTTT
CGTGACATTC
AGTTCGCTGC
GCTCACGGCT
CTGGCAGTGA
ATGGGGGTAA
ATGGCACTAC
AGGCGCCTTT
TATGGATTCA
TGCAAGGAAA
CTACCCATAA
TACAAGAAAA
GCCCGTCACG
GGCTTCTCAG
GGCGTTTTAT
GGCGGGTCTG
CTATGTGGTG
CTATCTGACT
TTTTGCTGTT
CAGCAGTTCC
TGCCCTCTGA
TTTTCCAGTC
TGACCACTTC
GGATTATCCC
GTGACAGGTC
ATTCAGACTG
GCTAATGCAC
CCAGTAAGGC
AGCGGTATCA
TCAACAGGCT
TACCCGTCTT
ACTGTCGGGG
ATCGACGCTC
TCCCTTATGC
GACTCCTGCA
CCTTTCGTCT
TCGAATAAAT
ACCTGTGACG
GAAGATCACT
TCGCAGAATA
AATAAATCCT
GGTGTCCCTG
TTGATACCGG
GAAGCCCTGG
GCCAACTTTT
GGCGAAAATG
AGACGTTGAT
CGGCACGTAA
GAGGTTCCAA
CTTTCACCAT
AATGAAATAA
GATCACTACC
GGGCGTATTT
TTTGAGTTAT
CGAGATTTTC
AGGAGCTAAG
GAAGCTAAAA
TGGAGAAAAA
AATCACTGGA
TATACCACCG
TTGATATATC
CCAATGGCAT
CGTAAAGAAC
ATTTTGAGGC
ATTTCAGTCA
GTTGCTCAAT
GTACCTATAA
CCAGACCGTT
CAGCTGGGTC
ATCCACCGGA
TCAATTCCCC
TGCTCGCGCA
GGCTGGGTGC
CAAGCTCTCG
GGTAACATCA
AGGCCCGATC
CTTGGAGCCC
TTGCCCTCCC
GCACGATGAT
CGTGCCGTGA
TCGAAATCCA
GATCCTTGAC
CCGCAGTTGC
AAACCCTCAC
TGATCCGGCT
CACGGTAACT
GATGCCGTAT
TTGCAGTACC
AGCGTACGGC
CCACAGAATG
ATGTCACGCT
GAAAATGCCG
GCCTTTGAAT
GGGTTCATGT
GCAGCTCCAT
CAGCAAAAGG
GGATGATAAG
TTTATCACCA
CCGACTATTT
GCAACAGTGC
CGTTGATCGT
GCTATGATCG
ACTGATGAAA
AAGCCTGAAC
TCACCGCGAC
GTCTGTCGAG
AAGTTTCTGA
TCGAAAAGTT
CGACAGCGTC
TCCGACCTGA
TGCAGCTCTC
GGAGGGCGAA
GAATCTCGTG
CTTTCAGCTT
CGATGTAGGA
GGGCGTGGAT
ATGTCCTGCG
GGTAAATAGC
TGCGCCGATG
GTTTCTACAA
AGATCGTTAT
GTTTATCGGC
ACTTTGCATC
GGCCGCGCTC
CCGATTCCGG
AAGTGCTTGA
CATTGGGGAA
TTCAGCGAGA
GCCTGACCTA
TTGCATCTCC
CGCCGTGCAC
AGGGTGTCAC
GTTGCAAGAC
CTGCCTGAAA
CCGAACTGCC
CGCTGTTCTG
CAGCCGGTCG
CGGAGGCCAT
GGATGCGATC
GCTGCGGCCG
ATCTTAGCCA
GACGAGCGGG
TTCGGCCCAT
TCGGACCGCA
AGGAATCGGT
CAATACACTA
CATGGCGTGA
TTTCATATGC
GCGATTGCTG
ATCCCCATGT
GTATCACTGG
CAAACTGTGA
TGGACGACAC
CGTCAGTGCG
TCCGTCGCGC
AGGCTCTCGA
TGAGCTGATG
CTTTGGGCCG
AGGACTGCCC
CGAAGTCCGG
CACCTCGTGC
ACGCGGATTT
CGGCTCCAAC
AATGTCCTGA
CGGACAATGG
CCGCATAACA
GCGGTCATTG
ACTGGAGCGA
GGCGATGTTC
GGGGATTCCC
AATACGAGGT
CGCCAACATC
TTCTTCTGGA
GGCCGTGGTT
GGCTTGTATG
GAGCAGCAGA
CGCGCTACTT
CGAGCGGAGG
CATCCGGAGC
TTGCAGGATC
GCCGCGGCTC
CGGGCGTATA
TGCTCCGCAT
TGGTCTTGAC
CAACTCTATC
AGAGCTTGGT
TGACGGCAAT
TTCGATGATG
CAGCTTGGGC
GCAGGGTCGA
TGCGACGCAA
TCGTCCGATC
CGGAGCCGGG
ACTGTCGGGC
GTACACAAAT
CGCCCGCAGA
AGCGCGGCCG
TCTGGACCGA
TGGCTGTGTA
GAAGTACTCG
CCGATAGTGG
AAACCGACGC
CCCAGCACTC
GTCCGAGGGC
AAAGGAATAG
TGAGCTCATG
AGCGGAGAAC
GAGATGACGT
TGGAGGGGCA
AGGTCGCGCT
GATTGCTGGG
GCAACACGTG
AAAGGCGAGA
TCACCAAGGT
AGTCGGCAAA
TAATGTCTAA
CGGTACCCGG
GGATCCTCTA
GAGTCGACCT
GCATAATGTG
CCTGTCAAAT
GGACGAAGCA
GGGATTCTGC
AAACCCTATG
CTACTCCGTC
AAGCCGTCAA
TTGTCTGATT
CGTTACCAAT
TATGACAACT
TGACGGCTAC
ATCATTCACT
TTTTCTTCAC
AACCGGCACG
GAACTCGCTC
GGGCTGGCCC
CGGTGCATTT
TTTAAATACC
CGCGAGAAAT
AGAGTTGATC
GTCAAAACCA
ACATTGCGAC
CGACGGTGGC
GATAGGCATC
CGGGTGGTGC
TCAAAAGCAG
CTTCGCCTGG
CTGATACGTT
GGTCCTCGCG
CCAGCTTAAG
ACGCTAATCC
CTAACTGCTG
GCGGAAAAGA
TGTGACAGAC
GCGACGGCGA
CAAGCAAACA
TGCTGTGCGA
CGCTGGCGAT
ATCAAAATTG
CTGTCTGCCA
GGTGATCGCT
GATGTACTGA
CAAGCCTCGC
GTACCCGATT
ATCCATCGGT
GGATGGAGCG
ACTCGTTAAT
CGCTTCCATG
CGCCGCAGTA
ACAATTGCTC
AAGCAGATTT
ATCGCCAGCA
GCTCCGAATA
GCGCCCTTCC
CCTTGCCCGG
CGTTAATGAT
TTGCCCAAAC
AGGTCGCTGA
AATGCGGCTG
GTGCGCTTCA
TCCGGGCGAA
AGAACCCCGT
ATTGGCAAAT
ATTGACGGCC
AGTTAAGCCA
TTCATGCCAG
TAGGCGCGCG
GACGAAAGTA
AACCCACTGG
TGATACCATT
CGCGAGCCTC
CGGATGACGA
CCGTAGTGAT
GAATCTCTCC
TGGCGGGAAC
AGCAAAATAT
CACCCGGTCG
GCAAACAAAT
TCTCGTCCCT
GATTTTTCAC
CACCCCCTGA
CCGCGAATGG
TGAGATTGAG
AATATAACCT
TTCATTCCCA
GCGGTCGGTC
GATAAAAAAA
TCGAGATAAC
CGTTGGCCTC
AATCGGCGTT
AAACCCGCCA
CCAGATGGGC
ATTAAACGAG
TATCCCGGCA
GCAGGGGATC
ATTTTGCGCT
TCAGCCATAC
TTTTCATACT
CCCGCCATTC
AGAGAAGAAA
CCAATTGTCC
ATATTGCATC
AGACATTGCC
GTCACTGCGT
CTTTTACTGG
CTCTTCTCGC
TAACCAAACC
GGTAACCCCG
CTTATTAAAA
GCATTCTGTA
ACAAAGCGGG
ACCAAAGCCA
TGACAAAAAC
GCGTAACAAA
AGTGTCTATA
ATCACGGCAG
AAAAGTCCAC
ATTGATTATT
TGCACGGCGT
CACACTTTGC
TATGCCATAG
CATTTTTATC
CATAAGATTA
GCGGATCCTA
CCTGACGCTT
TTTATCGCAA
CTCTCTACTG
TTTCTCCATA
CCCGTTTTTT
TGGGCTAGCG
AAAAGATGTT
TCGTGAAGCC
GTCGACGCTT
ATAAAAAATG
GATATTAATA
CTGAAACTGA
GATCAAGCAA
AAGCATTCAC
TAACCCCCTT
TCCTGTTTTC
CTAATCAGCC
CGGCATTTCG
CGGGCGATAT
TTTCACAGCT
ATTTCAGGAG
TTCAGCCATG
AACGCTTATT
ACATTCAGGA
TCGTCTTGAG
GCTCAGAGCT
GGGCGCGTCA
CTACCAGCAG
CTCGCCCGTG
AAGAGAAAGA
GGCAGAACTG
GCAGACGACA
TGGAAAAAGG
CCTGCCCCAG
CACCTGTTTG
AATCGCTATG
CATCGATCAT
TTGCAACGCC
ACGGGGCCAG
CAAAAAATCC
ATTACCCGTG
CGTTTGATGA
CGATGTTGAG
TTTCAGGAGC
GCATGGCAGA
ACACATCCGG
TACATGGTTG
AAACCATTGC
TCACCACCAG
GTTGATATTG
ATTCAGAGGT
ATAAAACGAA
TGAGTACTGC
ACTCGCAACG
CTGGCTGGGA
AGCTGGCTGA
ACGTGTCGGC
ATGGATTCTG
TCGACCCACA
GGAACTGATC
ACCACTCTTC
GCCAGACGGC
ATTTAAAGGT
GATGCCAGCG
ATGCGCAGTT
CATCGCATTA
CTGATCGTTG
CCAACCAGTA
CGGCCTTAAT
CCGTGGACGA
AAGAAATTTA
CGCCTTTCCT
GATAAGCAGA
ATGGCATCGT
TCCGGTGGTG
GGCGTTGATG
GCTGGTCCCG
CATCATCAAT
GAAAACCAGC
AGTTTGATGG
CATGGACTTT
GAGCAGGACA
ATGAATCCTG
TACATGCCGG
ATTTACCGCA
AGGACCGTAA
TCATCCGATC
TGCGTTACCG
AATGGATGGA
TGAATGCCGC
CGCGAACCAT
TCAAAACTCG
CGAAGGCAGA
GAAATCACGG
GGCCGTGGCA
GTCGCATCCC
AAACGGATGT
TACGTCATAA
AGCCATGATT
CAGTGTGCCC
GTCTGGCCTT
CGGATTTGCT
GGTATCTATG
ACAAGGATGA
AGCCGAGCGC
ATTGTCGAAA
ATACTGCATA
CACTGCAGAA
CGTCAGCCGG
AACGCGACAT
CACTCCGGTT
AACGATGAAA
CCATGCAGGA
GATTAACACT
CTGCTGATCG
CCCTGGATAA
AACATGGGAT
GACGACTTAT
TGCCGCTCTG
TTCCCAGATA
TTTCGCCGCG
ACATTCGTGC
ATCGTCAGAA
CTGACACAGG
CCGAAGCAGT
AAAAGCTCTT
GGATTCCTGA
AACAGAAAGC
CGCAGAGCAG
AAGGTGGCAG
CATGACACCG
GACATTATCC
TGCAGCGTAC
CGGGATCGAT
GTGAGAGCTG
TCGAACAGGG
GGATGATGCG
TGGCACAAAT
TACGGCTCGG
CGTCATCACC
GCTTCAGAAG
TTCACAACGT
GATAGCAAAA
CCCCGCTCCG
GAAAGAAGTG
GCCTGACATG
AAAATGTCCT
ACTTCCACAC
CCTGCTTGCT
GAGGTTTGCA
CCGGTGTGGC
TCCGGAAGTT
AACGCTAAAG
CACTGGCCTG
GGGAAAACAG
TACGAGAACG
ACGCCAGAAC
CCTGTTTGAA
TTCACTTCCG
GCGTGAATGT
TACTGAATCC
CCGATCATCT
ATCGCGACGA
AAGTATGCGT
ACCGCCTGCT
CTCCCGATGG
TTTATGCAGT
GACGGCAACG
GCCTTGAACT
GAAATGCCCG
TTTACCTCCC
GGGATTTCAT
GAAGTTCCGG
CTCGGTGGTT
TCGAGGCCAT
AAAGTCAGCT
TACATGGCCC
AGGTGCAGTA
CAGCATGTGG
GTGACGCGAA
AAAATGCCTG
GTACTTTGCC
AACTATGACC
CGCGTATGAA
GCGTGAAGGC
CTGCATTATG
TCGTGATTGA
GCGGGATGAA
AAGTACATGG
CGAGTTTTGA
CGAGATCGTG
CCGGAGTTCA
TCGAAAAAAT
GGACGAGGCA
CTGGCTGAAA
TTGGTTTTGT
ATTTGGGGAG
CAATGGCGAT
GACGCATCCT
CACGATAATA
AGCTTCCTGC
TGAACATCAA
AGGCAAGAAA
ACATCTGTTG
TCAAAGACAG
CATCCTTGAA
CAAGGACAAT
TAACAGTTAA
CAAATAAAAA
CGCAAAAGAA
AATGCCGATA
TTGACTACCG
GAAGCAGTGT
GACCGTGTGC
TTCTCAAATG
CCTGATTCAG
GCTGTCTATG
TGTGACTGTT
GAGCTGTAAC
AAGTTGTCTC
AGGTGTTCAA
TTTCATGTTC
TAGTTGCTTT
GTTTTACTGG
TTTCACCTGT
TCTATTAGGT
GTTACATGCT
GTTCATCTGT
TACATTGTCG
ATCTGTTCAT
GGTGAACAGC
TTTAAATGCA
CCAAAAACTC
GTAAAAGCTC
TGATGTATCT
ATCTTTTTTA
CACCGTTTTC
ATCTGTGCAT
ATAGCTATAC
TGATTTCGTC
AGACTCACAG
TCAAACATGC
CGGTCAGTTG
GCCTGGTGAT
GGCGGGATCG
TTGTATATTT
CTTGACACCT
TTTCGGCACC
GCCCTAAAAT
TCTGCGTCCT
CATAATATAT
GAGGCGATTT
ATTACGTGTT
TACGAAGCAA
AAGCTAAAAC
CAGGAGCTAT
TTAATGGATA
AGAAATACTC
AATAGGCTTA
GATATCGGCA
CAAATAGCGT
CGGATGGGCG
GTGATCACTG
ATGAATATAA
GGTTCCGTCT
AAAAAGTTCA
AGGTTCTGGG
AAATACAGAC
CGCCACAGTA
TCAAAAAAAA
TCTTATAGGG
GCTCTTTTAT
TTGACAGTGG
AGAGACAGCG
GAAGCGACTC
GTCTCAAACG
GACAGCTCGT
AGAAGGTATA
CACGTCGGAA
GAATCGTATT
TGTTATCTAC
AGGAGATTTT
TTCAAATGAG
ATGGCGAAAG
TAGATGATAG
TTTCTTTCAT
CGACTTGAAG
AGTCTTTTTT
GGTGGAAGAA
GACAAGAAGC
ATGAACGTCA
TCCTATTTTT
GGAAATATAG
TAGATGAAGT
TGCTTATCAT
GAGAAATATC
CAACTATCTA
TCATCTGCGA
AAAAAATTGG
TAGATTCTAC
TGATAAAGCG
GATTTGCGCT
TAATCTATTT
GGCCTTAGCG
CATATGATTA
AGTTTCGTGG
TCATTTTTTG
ATTGAGGGAG
ATTTAAATCC
TGATAATAGT
GATGTGGACA
AACTATTTAT
CCAGTTGGTA
CAAACCTACA
ATCAATTATT
TGAAGAAAAC
CCTATTAACG
CAAGTGGAGT
AGATGCTAAA
GCGATTCTTT
CTGCACGATT
GAGTAAATCA
AGACGATTAG
AAAATCTCAT
TGCTCAGCTC
CCCGGTGAGA
AGAAAAATGG
CTTATTTGGG
AATCTCATTG
CTTTGTCATT
GGGTTTGACC
CCTAATTTTA
AATCAAATTT
TGATTTGGCA
GAAGATGCTA
AATTACAGCT
TTCAAAAGAT
ACTTACGATG
ATGATTTAGA
TAATTTATTG
GCGCAAATTG
GAGATCAATA
TGCTGATTTG
TTTTTGGCAG
CTAAGAATTT
ATCAGATGCT
ATTTTACTTT
CAGATATCCT
AAGAGTAAAT
ACTGAAATAA
CTAAGGCTCC
CCTATCAGCT
TCAATGATTA
AACGCTACGA
TGAACATCAT
CAAGACTTGA
CTCTTTTAAA
AGCTTTAGTT
CGACAACAAC
TTCCAGAAAA
GTATAAAGAA
ATCTTTTTTG
ATCAATCAAA
AAACGGATAT
GCAGGTTATA
TTGATGGGGG
AGCTAGCCAA
GAAGAATTTT
ATAAATTTAT
CAAACCAATT
TTAGAAAAAA
TGGATGGTAC
TGAGGAATTA
TTGGTGAAAC
TAAATCGTGA
AGATTTGCTG
CGCAAGCAAC
GGACCTTTGA
CAACGGCTCT
ATTCCCCATC
AAATTCACTT
GGGTGAGCTG
CATGCTATTT
TGAGAAGACA
AGAAGACTTT
TATCCATTTT
TAAAAGACAA
TCGTGAGAAG
ATTGAAAAAA
TCTTGACTTT
TCGAATTCCT
TATTATGTTG
GTCCATTGGC
GCGTGGCAAT
AGTCGTTTTG
CATGGATGAC
TCGGAAGTCT
GAAGAAACAA
TTACCCCATG
GAATTTTGAA
GAAGTTGTCG
ATAAAGGTGC
TTCAGCTCAA
TCATTTATTG
AACGCATGAC
AAACTTTGAT
AAAAATCTTC
CAAATGAAAA
AGTACTACCA
AAACATAGTT
TGCTTTATGA
GTATTTTACG
GTTTATAACG
AATTGACAAA
GGTCAAATAT
GTTACTGAAG
GAATGCGAAA
ACCAGCATTT
CTTTCAGGTG
AACAGAAGAA
AGCCATTGTT
GATTTACTCT
TCAAAACAAA
TCGAAAAGTA
ACCGTTAAGC
AATTAAAAGA
AGATTATTTC
AAAAAAATAG
AATGTTTTGA
TAGTGTTGAA
ATTTCAGGAG
TTGAAGATAG
ATTTAATGCT
TCATTAGGTA
CCTACCATGA
TTTGCTAAAA
ATTATTAAAG
ATAAAGATTT
TTTGGATAAT
GAAGAAAATG
AAGATATCTT
AGAGGATATT
GTTTTAACAT
TGACCTTATT
TGAAGATAGG
GAGATGATTG
AGGAAAGACT
TAAAACATAT
GCTCACCTCT
TTGATGATAA
GGTGATGAAA
CAGCTTAAAC
GTCGCCGTTA
TACTGGTTGG
GGACGTTTGT
CTCGAAAATT
GATTAATGGT
ATTAGGGATA
AGCAATCTGG
CAAAACAATA
TTAGATTTTT
TGAAATCAGA
TGGTTTTGCC
AATCGCAATT
TTATGCAGCT
GATCCATGAT
GATAGTTTGA
CATTTAAAGA
AGACATTCAA
AAAGCACAAG
TGTCTGGACA
AGGCGATAGT
TTACATGAAC
ATATTGCAAA
TTTAGCTGGT
AGCCCTGCTA
TTAAAAAAGG
TATTTTACAG
ACTGTAAAAG
TTGTTGATGA
ATTGGTCAAA
GTAATGGGGC
GGCATAAGCC
AGAAAATATC
GTTATTGAAA
TGGCACGTGA
AAATCAGACA
ACTCAAAAGG
GCCAGAAAAA
TTCGCGAGAG
CGTATGAAAC
GAATCGAAGA
AGGTATCAAA
GAATTAGGAA
GTCAGATTCT
TAAAGAGCAT
CCTGTTGAAA
ATACTCAATT
GCAAAATGAA
AAGCTCTATC
TCTATTATCT
CCAAAATGGA
AGAGACATGT
ATGTGGACCA
AGAATTAGAT
ATTAATCGTT
TAAGTGATTA
TGATGTCGAT
CACATTGTTC
CACAAAGTTT
CCTTAAAGAC
GATTCAATAG
ACAATAAGGT
CTTAACGCGT
TCTGATAAAA
ATCGTGGTAA
ATCGGATAAC
GTTCCAAGTG
AAGAAGTAGT
CAAAAAGATG
AAAAACTATT
GGAGACAACT
TCTAAACGCC
AAGTTAATCA
CTCAACGTAA
GTTTGATAAT
TTAACGAAAG
CTGAACGTGG
AGGTTTGAGT
GAACTTGATA
AAGCTGGTTT
TATCAAACGC
CAATTGGTTG
AAACTCGCCA
AATCACTAAG
CATGTGGCAC
AAATTTTGGA
TAGTCGCATG
AATACTAAAT
ACGATGAAAA
TGATAAACTT
ATTCGAGAGG
TTAAAGTGAT
TACCTTAAAA
TCTAAATTAG
TTTCTGACTT
CCGAAAAGAT
TTCCAATTCT
ATAAAGTACG
TGAGATTAAC
AATTACCATC
ATGCCCATGA
TGCGTATCTA
AATGCCGTCG
TTGGAACTGC
TTTGATTAAG
AAATATCCAA
AACTTGAATC
GGAGTTTGTC
TATGGTGATT
ATAAAGTTTA
TGATGTTCGT
AAAATGATTG
CTAAGTCTGA
GCAAGAAATA
GGCAAAGCAA
CCGCAAAATA
TTTCTTTTAC
TCTAATATCA
TGAACTTCTT
CAAAACAGAA
ATTACACTTG
CAAATGGAGA
GATTCGCAAA
CGCCCTCTAA
TCGAAACTAA
TGGGGAAACT
GGAGAAATTG
TCTGGGATAA
AGGGCGAGAT
TTTGCCACAG
TGCGCAAAGT
ATTGTCCATG
CCCCAAGTCA
ATATTGTCAA
GAAAACAGAA
GTACAGACAG
GCGGATTCTC
CAAGGAGTCA
ATTTTACCAA
AAAGAAATTC
GGACAAGCTT
ATTGCTCGTA
AAAAAGACTG
GGATCCAAAA
AAATATGGTG
GTTTTGATAG
TCCAACGGTA
GCTTATTCAG
TCCTAGTGGT
TGCTAAGGTG
GAAAAAGGGA
AATCGAAGAA
GTTAAAATCC
GTTAAAGAGT
TACTAGGGAT
CACAATTATG
GAAAGAAGTT
CCTTTGAAAA
AAATCCGATT
GACTTTTTAG
AAGCTAAAGG
ATATAAGGAA
GTTAAAAAAG
ACTTAATCAT
TAAACTACCT
AAATATAGTC
TTTTTGAGTT
AGAAAACGGT
CGTAAACGGA
TGCTGGCTAG
TGCCGGAGAA
TTACAAAAAG
GAAATGAGCT
GGCTCTGCCA
AGCAAATATG
TGAATTTTTT
ATATTTAGCT
AGTCATTATG
AAAAGTTGAA
GGGTAGTCCA
GAAGATAACG
AACAAAAACA
ATTGTTTGTG
GAGCAGCATA
AGCATTATTT
AGATGAGATT
ATTGAGCAAA
TCAGTGAATT
TTCTAAGCGT
GTTATTTTAG
CAGATGCCAA
TTTAGATAAA
GTTCTTAGTG
CATATAACAA
ACATAGAGAC
AAACCAATAC
GTGAACAAGC
AGAAAATATT
ATTCATTTAT
TTACGTTGAC
GAATCTTGGA
GCTCCCGCTG
CTTTTAAATA
TTTTGATACA
ACAATTGATC
GTAAACGATA
TACGTCTACA
AAAGAAGTTT
TAGATGCCAC
TCTTATCCAT
CAATCCATCA
CTGGTCTTTA
TGAAACACGC
ATTGATTTGA
GTCAGCTAGG
AGGTGACTGA
TGGCTGGTTG
GCGTACTGTT
GTGGTAAATA
CCCATATGGA
CAGTTTTCCC
TTTGATATGT
AACGGTGAAC
AGTTGTTCTA
CTTTTGTTTG
TTAGTCTTGA
TGCTTCACTG
ATAGATACAA
GAGCCATAAG
AACC