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pEJS654 All-in-One AAV-sgRNA-hNmeCas9

产品货号:P5593

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pEJS654 All-in-One AAV-sgRNA-hNmeCas9
别称: Plasmid#112139
基因长:
质粒宿主: 哺乳细胞,腺相关病毒
质粒用途: 基因编辑
片段类型: CRISPR
片段物种:
原核抗性: Amp
筛选标记:
荧光标记:
启动子:
复制子:
拷贝数:
感受态: Stbl3
温度: 37度
背景载体:
正向引物:
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

CCTGCAGGCA

GCTGCGCGCT

CGCTCGCTCA

CTGAGGCCGC

CCGGGCGTCG

GGCGACCTTT

61

GGTCGCCCGG

CCTCAGTGAG

CGAGCGAGCG

CGCAGAGAGG

GAGTGGCCAA

CTCCATCACT

121

AGGGGTTCCT

GCGGCCTCTA

GAGTTTAAAC

AAAAAAATAA

ACGATGCCCC

TTAAAGCAGA

181

AGCTTTAAGG

GGCAGAGCGT

TGCGGCACAT

CTTTTCAGAC

GGCCTTATTG

TAGCAACGGT

241

TCTCATTTCG

TTTCCGAAAT

GAGAAAGGGA

GCTACAACTG

AAGAGCTGGA

ATGCTCTTCC

301

GGTGTTTCGT

CCTTTCCACA

AGATATATAA

AGCCAAGAAA

TCGAAATACT

TTCAAGTTAC

361

GGTAAGCATA

TGATAGTCCA

TTTTAAAACA

TAATTTTAAA

ACTGCAAACT

ACCCAAGAAA

421

TTATTACTTT

CTACGTCACG

TATTTTGTAC

TAATATCTTT

GTGTTTACAG

TCAAATTAAT

481

TCTAATTATC

TCTCTAACAG

CCTTGTATCG

TATATGCAAA

TATGAAGGAA

TCATGGGAAA

541

TAGGCCCTCT

TCCTGCCCGA

CCTTGACGTC

GACTCTAGAA

TGGAGGCGGT

ACTATGTAGA

601

TGAGAATTCA

GGAGCAAACT

GGGAAAAGCA

ACTGCTTCCA

AATATTTGTG

ATTTTTACAG

661

TGTAGTTTTG

GAAAAACTCT

TAGCCTACCA

ATTCTTCTAA

GTGTTTTAAA

ATGTGGGAGC

721

CAGTACACAT

GAAGTTATAG

AGTGTTTTAA

TGAGGCTTAA

ATATTTACCG

TAACTATGAA

781

ATGCTACGCA

TATCATGCTG

TTCAGGCTCC

GTGGCCACGC

AACTCATACT

TAAGCAGACA

841

GTGGTTCAAA

GTTTTTTTCT

TCCATTTCAG

GTGTCGTGAA

CACCGCCACC

ATGGTGCCTA

901

AGAAGAAGAG

AAAGGTGGAA

GATAAACGCC

CAGCAGCTAC

AAAGAAGGCA

GGTCAAGCCA

961

AGAAAAAGAA

AGCAGCATTC

AAGCCAAACT

CAATCAATTA

CATCCTGGGA

CTGGACATCG

1021

GCATCGCATC

CGTCGGGTGG

GCTATGGTCG

AAATCGACGA

GGAGGAGAAC

CCCATCCGCC

1081

TGATCGATCT

GGGCGTGCGC

GTGTTTGAGA

GGGCAGAGGT

GCCTAAGACC

GGCGACAGCC

1141

TGGCCATGGC

ACGGAGACTG

GCACGCTCCG

TGAGGCGCCT

GACCCGGAGA

AGGGCCCACA

1201

GACTGCTGAG

GACACGCCGG

CTGCTGAAGA

GGGAGGGCGT

GCTGCAGGCC

GCCAACTTCG

1261

ATGAGAATGG

CCTGATCAAG

TCCCTGCCCA

ATACCCCTTG

GCAGCTGAGG

GCAGCCGCCC

1321

TGGACCGCAA

GCTGACACCT

CTGGAGTGGT

CCGCCGTGCT

GCTGCACCTG

ATCAAGCACC

1381

GGGGCTACCT

GTCTCAGAGA

AAGAACGAGG

GCGAGACAGC

CGATAAGGAG

CTGGGCGCCC

1441

TGCTGAAGGG

AGTGGCAGGA

AATGCACACG

CCCTGCAGAC

CGGCGACTTT

CGCACACCAG

1501

CCGAGCTGGC

CCTGAACAAG

TTCGAGAAGG

AGAGCGGCCA

CATCCGCAAT

CAGCGGTCTG

1561

ACTATAGCCA

CACCTTCTCC

CGGAAGGATC

TGCAGGCCGA

GCTGATCCTG

CTGTTTGAGA

1621

AGCAGAAGGA

GTTCGGCAAC

CCACACGTGT

CTGGCGGCCT

GAAGGAGGGC

ATCGAGACAC

1681

TGCTGATGAC

ACAGCGGCCC

GCCCTGAGCG

GCGACGCAGT

GCAGAAGATG

CTGGGACACT

1741

GCACCTTTGA

GCCAGCCGAG

CCCAAGGCCG

CCAAGAATAC

CTACACAGCC

GAGCGGTTCA

1801

TCTGGCTGAC

AAAGCTGAAC

AATCTGAGGA

TCCTGGAGCA

GGGAAGCGAG

CGCCCACTGA

1861

CCGACACAGA

GAGGGCCACC

CTGATGGATG

AGCCCTACCG

CAAGTCCAAG

CTGACATATG

1921

CACAGGCAAG

GAAGCTGCTG

GGCCTGGAGG

ACACCGCCTT

CTTTAAGGGC

CTGAGATACG

1981

GCAAGGATAA

CGCCGAGGCC

TCTACACTGA

TGGAGATGAA

GGCCTATCAC

GCCATCAGCA

2041

GGGCCCTGGA

GAAGGAGGGC

CTGAAGGACA

AGAAGTCCCC

ACTGAATCTG

TCTCCCGAGC

2101

TGCAGGATGA

GATCGGCACC

GCCTTTAGCC

TGTTCAAGAC

CGACGAGGAT

ATCACAGGCA

2161

GACTGAAGGA

CAGGATCCAG

CCAGAGATCC

TGGAGGCCCT

GCTGAAGCAC

ATCAGCTTTG

2221

ATAAGTTCGT

GCAGATCAGC

CTGAAGGCCC

TGCGGAGGAT

CGTGCCACTG

ATGGAGCAGG

2281

GCAAGAGGTA

CGACGAGGCC

TGCGCCGAAA

TCTACGGCGA

TCACTATGGC

AAGAAGAACA

2341

CAGAGGAGAA

AATCTACCTG

CCCCCTATCC

CCGCCGATGA

GATCAGGAAC

CCTGTGGTGC

2401

TGCGCGCCCT

GTCTCAGGCA

AGAAAAGTGA

TCAACGGAGT

GGTGCGCCGG

TACGGCAGCC

2461

CCGCCAGAAT

CCACATCGAG

ACAGCCAGGG

AAGTGGGCAA

GTCCTTTAAG

GACAGAAAGG

2521

AGATCGAGAA

GAGGCAGGAG

GAGAACAGAA

AGGATAGGGA

GAAGGCCGCC

GCCAAGTTCA

2581

GAGAGTACTT

TCCTAATTTC

GTGGGCGAGC

CAAAGTCCAA

GGATATCCTG

AAGCTGAGGC

2641

TGTACGAGCA

GCAGCACGGC

AAGTGTCTGT

ATTCTGGCAA

GGAGATCAAC

CTGGGCCGCC

2701

TGAATGAGAA

GGGCTATGTG

GAGATCGACC

ACGCCCTGCC

TTTTTCTCGG

ACCTGGGACG

2761

ATAGCTTCAA

CAATAAGGTG

CTGGTGCTGG

GCTCTGAGAA

CCAGAATAAG

GGCAACCAGA

2821

CACCCTACGA

GTATTTCAAC

GGCAAGGACA

ATAGCCGCGA

GTGGCAGGAG

TTTAAGGCAA

2881

GGGTGGAGAC

AAGCAGGTTC

CCTCGGTCCA

AGAAGCAGAG

AATCCTGCTG

CAGAAGTTTG

2941

ACGAGGATGG

CTTCAAGGAG

AGGAACCTGA

ATGACACCCG

CTACGTGAAT

CGGTTTCTGT

3001

GCCAGTTCGT

GGCCGATAGA

ATGAGGCTGA

CCGGCAAGGG

CAAGAAGAGA

GTGTTTGCCT

3061

CCAACGGCCA

GATCACAAAT

CTGCTGAGGG

GCTTCTGGGG

CCTGAGAAAG

GTGAGGGCAG

3121

AGAACGACAG

GCACCACGCA

CTGGATGCAG

TGGTGGTGGC

ATGTTCTACC

GTGGCCATGC

3181

AGCAGAAGAT

CACACGCTTT

GTGCGGTATA

AGGAGATGAA

TGCCTTCGAC

GGCAAGACCA

3241

TCGATAAGGA

GACAGGCGAG

GTGCTGCACC

AGAAGACACA

CTTTCCTCAG

CCATGGGAGT

3301

TCTTTGCCCA

GGAAGTGATG

ATCCGGGTGT

TTGGCAAGCC

TGACGGCAAG

CCAGAGTTCG

3361

AGGAGGCCGA

TACCCTGGAG

AAGCTGAGAA

CACTGCTGGC

AGAGAAGCTG

AGCTCCAGGC

3421

CCGAGGCAGT

GCACGAGTAC

GTGACCCCAC

TGTTCGTGTC

TAGAGCCCCC

AACAGGAAGA

3481

TGAGCGGCCA

GGGCCACATG

GAGACAGTGA

AGTCCGCCAA

GAGACTGGAC

GAGGGCGTGT

3541

CTGTGCTGAG

GGTGCCTCTG

ACACAGCTGA

AGCTGAAGGA

TCTGGAGAAG

ATGGTGAATC

3601

GCGAGCGGGA

GCCAAAGCTG

TATGAGGCCC

TGAAGGCAAG

GCTGGAGGCA

CACAAGGACG

3661

ATCCTGCCAA

GGCCTTTGCC

GAGCCATTCT

ACAAGTATGA

TAAGGCCGGC

AACAGAACCC

3721

AGCAGGTGAA

GGCCGTGAGG

GTGGAGCAGG

TGCAGAAGAC

AGGCGTGTGG

GTGCGCAACC

3781

ACAATGGCAT

CGCCGACAAT

GCTACCATGG

TGCGGGTGGA

CGTGTTTGAG

AAGGGCGATA

3841

AGTACTATCT

GGTGCCCATC

TACAGCTGGC

AGGTGGCCAA

GGGCATCCTG

CCTGATAGAG

3901

CCGTGGTGCA

GGGCAAGGAC

GAGGAGGATT

GGCAGCTGAT

CGACGATTCC

TTCAACTTTA

3961

AGTTCTCTCT

GCACCCCAAT

GACCTGGTGG

AAGTGATCAC

CAAGAAGGCC

AGGATGTTTG

4021

GCTACTTCGC

CTCCTGCCAC

CGCGGCACAG

GCAACATCAA

TATCCGGATC

CACGACCTGG

4081

ATCACAAGAT

CGGCAAGAAC

GGCATCCTGG

AGGGCATCGG

CGTGAAGACA

GCCCTGAGCT

4141

TCCAGAAGTA

TCAGATCGAC

GAGCTGGGCA

AGGAGATCAG

ACCTTGTAGG

CTGAAGAAGC

4201

GCCCACCCGT

GCGGGAGGAT

AAGCGGCCCG

CAGCAACCAA

GAAGGCAGGA

CAGGCCAAGA

4261

AGAAGAAGTA

CCCCTATGAC

GTGCCTGACT

ACGCCGGGTA

TCCCTACGAC

GTGCCTGATT

4321

ACGCCGGGTC

CTATCCCTAC

GACGTGCCAG

ATTACGCTGC

AGCTCCAGCA

GCGAAGAAAA

4381

AGAAGCTGGA

TTAAGATCTT

TTTCCCTCTG

CCAAAAATTA

TGGGGACATC

ATGAAGCCCC

4441

TTGAGCATCT

GACTTCTGGC

TAATAAAGGA

AATTTATTTT

CATTGCAATA

GTGTGTTGGA

4501

ATTTTTTGTG

TCTCTCACTC

GGCGGCCGCA

GGAACCCCTA

GTGATGGAGT

TGGCCACTCC

4561

CTCTCTGCGC

GCTCGCTCGC

TCACTGAGGC

CGGGCGACCA

AAGGTCGCCC

GACGCCCGGG

4621

CTTTGCCCGG

GCGGCCTCAG

TGAGCGAGCG

AGCGCGCAGC

TGCCTGCAGG

GGCGCCTGAT

4681

GCGGTATTTT

CTCCTTACGC

ATCTGTGCGG

TATTTCACAC

CGCATACGTC

AAAGCAACCA

4741

TAGTACGCGC

CCTGTAGCGG

CGCATTAAGC

GCGGCGGGTG

TGGTGGTTAC

GCGCAGCGTG

4801

ACCGCTACAC

TTGCCAGCGC

CTTAGCGCCC

GCTCCTTTCG

CTTTCTTCCC

TTCCTTTCTC

4861

GCCACGTTCG

CCGGCTTTCC

CCGTCAAGCT

CTAAATCGGG

GGCTCCCTTT

AGGGTTCCGA

4921

TTTAGTGCTT

TACGGCACCT

CGACCCCAAA

AAACTTGATT

TGGGTGATGG

TTCACGTAGT

4981

GGGCCATCGC

CCTGATAGAC

GGTTTTTCGC

CCTTTGACGT

TGGAGTCCAC

GTTCTTTAAT

5041

AGTGGACTCT

TGTTCCAAAC

TGGAACAACA

CTCAACTCTA

TCTCGGGCTA

TTCTTTTGAT

5101

TTATAAGGGA

TTTTGCCGAT

TTCGGTCTAT

TGGTTAAAAA

ATGAGCTGAT

TTAACAAAAA

5161

TTTAACGCGA

ATTTTAACAA

AATATTAACG

TTTACAATTT

TATGGTGCAC

TCTCAGTACA

5221

ATCTGCTCTG

ATGCCGCATA

GTTAAGCCAG

CCCCGACACC

CGCCAACACC

CGCTGACGCG

5281

CCCTGACGGG

CTTGTCTGCT

CCCGGCATCC

GCTTACAGAC

AAGCTGTGAC

CGTCTCCGGG

5341

AGCTGCATGT

GTCAGAGGTT

TTCACCGTCA

TCACCGAAAC

GCGCGAGACG

AAAGGGCCTC

5401

GTGATACGCC

TATTTTTATA

GGTTAATGTC

ATGATAATAA

TGGTTTCTTA

GACGTCAGGT

5461

GGCACTTTTC

GGGGAAATGT

GCGCGGAACC

CCTATTTGTT

TATTTTTCTA

AATACATTCA

5521

AATATGTATC

CGCTCATGAG

ACAATAACCC

TGATAAATGC

TTCAATAATA

TTGAAAAAGG

5581

AAGAGTATGA

GTATTCAACA

TTTCCGTGTC

GCCCTTATTC

CCTTTTTTGC

GGCATTTTGC

5641

CTTCCTGTTT

TTGCTCACCC

AGAAACGCTG

GTGAAAGTAA

AAGATGCTGA

AGATCAGTTG

5701

GGTGCACGAG

TGGGTTACAT

CGAACTGGAT

CTCAACAGCG

GTAAGATCCT

TGAGAGTTTT

5761

CGCCCCGAAG

AACGTTTTCC

AATGATGAGC

ACTTTTAAAG

TTCTGCTATG

TGGCGCGGTA

5821

TTATCCCGTA

TTGACGCCGG

GCAAGAGCAA

CTCGGTCGCC

GCATACACTA

TTCTCAGAAT

5881

GACTTGGTTG

AGTACTCACC

AGTCACAGAA

AAGCATCTTA

CGGATGGCAT

GACAGTAAGA

5941

GAATTATGCA

GTGCTGCCAT

AACCATGAGT

GATAACACTG

CGGCCAACTT

ACTTCTGACA

6001

ACGATCGGAG

GACCGAAGGA

GCTAACCGCT

TTTTTGCACA

ACATGGGGGA

TCATGTAACT

6061

CGCCTTGATC

GTTGGGAACC

GGAGCTGAAT

GAAGCCATAC

CAAACGACGA

GCGTGACACC

6121

ACGATGCCTG

TAGCAATGGC

AACAACGTTG

CGCAAACTAT

TAACTGGCGA

ACTACTTACT

6181

CTAGCTTCCC

GGCAACAATT

AATAGACTGG

ATGGAGGCGG

ATAAAGTTGC

AGGACCACTT

6241

CTGCGCTCGG

CCCTTCCGGC

TGGCTGGTTT

ATTGCTGATA

AATCTGGAGC

CGGTGAGCGT

6301

GGAAGCCGCG

GTATCATTGC

AGCACTGGGG

CCAGATGGTA

AGCCCTCCCG

TATCGTAGTT

6361

ATCTACACGA

CGGGGAGTCA

GGCAACTATG

GATGAACGAA

ATAGACAGAT

CGCTGAGATA

6421

GGTGCCTCAC

TGATTAAGCA

TTGGTAACTG

TCAGACCAAG

TTTACTCATA

TATACTTTAG

6481

ATTGATTTAA

AACTTCATTT

TTAATTTAAA

AGGATCTAGG

TGAAGATCCT

TTTTGATAAT

6541

CTCATGACCA

AAATCCCTTA

ACGTGAGTTT

TCGTTCCACT

GAGCGTCAGA

CCCCGTAGAA

6601

AAGATCAAAG

GATCTTCTTG

AGATCCTTTT

TTTCTGCGCG

TAATCTGCTG

CTTGCAAACA

6661

AAAAAACCAC

CGCTACCAGC

GGTGGTTTGT

TTGCCGGATC

AAGAGCTACC

AACTCTTTTT

6721

CCGAAGGTAA

CTGGCTTCAG

CAGAGCGCAG

ATACCAAATA

CTGTTCTTCT

AGTGTAGCCG

6781

TAGTTAGGCC

ACCACTTCAA

GAACTCTGTA

GCACCGCCTA

CATACCTCGC

TCTGCTAATC

6841

CTGTTACCAG

TGGCTGCTGC

CAGTGGCGAT

AAGTCGTGTC

TTACCGGGTT

GGACTCAAGA

6901

CGATAGTTAC

CGGATAAGGC

GCAGCGGTCG

GGCTGAACGG

GGGGTTCGTG

CACACAGCCC

6961

AGCTTGGAGC

GAACGACCTA

CACCGAACTG

AGATACCTAC

AGCGTGAGCT

ATGAGAAAGC

7021

GCCACGCTTC

CCGAAGGGAG

AAAGGCGGAC

AGGTATCCGG

TAAGCGGCAG

GGTCGGAACA

7081

GGAGAGCGCA

CGAGGGAGCT

TCCAGGGGGA

AACGCCTGGT

ATCTTTATAG

TCCTGTCGGG

7141

TTTCGCCACC

TCTGACTTGA

GCGTCGATTT

TTGTGATGCT

CGTCAGGGGG

GCGGAGCCTA

7201

TGGAAAAACG

CCAGCAACGC

GGCCTTTTTA

CGGTTCCTGG

CCTTTTGCTG

GCCTTTTGCT

7261

CACATGT

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