名称: | pCMV-LINC01106(human)-ncRNA-EGFP |
---|---|
别称: | |
基因长: | 2204bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 转录调控 |
片段类型: | ncRNA |
片段物种: | 人 |
原核抗性: | Kan |
筛选标记: | Neo/G418 |
荧光标记: | 绿色 |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TAGTTATTAA
TAGTAATCAA
TTACGGGGTC
ATTAGTTCAT
AGCCCATATA
TGGAGTTCCG
CGTTACATAA
CTTACGGTAA
ATGGCCCGCC
TGGCTGACCG
CCCAACGACC
CCCGCCCATT
GACGTCAATA
ATGACGTATG
TTCCCATAGT
AACGCCAATA
GGGACTTTCC
ATTGACGTCA
ATGGGTGGAG
TATTTACGGT
AAACTGCCCA
CTTGGCAGTA
CATCAAGTGT
ATCATATGCC
AAGTACGCCC
CCTATTGACG
TCAATGACGG
TAAATGGCCC
GCCTGGCATT
ATGCCCAGTA
CATGACCTTA
TGGGACTTTC
CTACTTGGCA
GTACATCTAC
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTGG
TTTAGTGAAC
CGTCAGATCC
GCTAGCGCTA
CCGGACTCAG
ATCTCGAGCT
CCGAATTCGC
GATCGCTGCA
GTCGACGGTA
CCGCAAGCTT
ATATTGAGCG
AACTCTTCTG
CGGGAAGGGC
TTGGCGTAGA
CCGCGCACTG
GAAGGCGTGC
GCGCAGGTGT
GCGTGACCAT
GTGCTTGAAA
CGGCAGTAGC
GCAGCGAGAA
GGATCGCCAT
CACACGGCGC
ACTGGTGCGG
CTTCTCCCCC
GAGTGGATGA
ACATGTGCTT
GGTGTCGTTT
TTCCTTGAGC
TGAACGTCTT
GCAACAGTGG
CCGCACGCAT
AGGGCTGACC
CCACATTGGA
CACCGCTGCA
TCCATGTCCA
TCACACAACA
CAGCTGCCGT
TTCTTCTGCC
TGCTTATGGG
AAAGTCCCCT
CTTCTCCTCC
GTTTTCTTCT
CTTCCTGCCC
TATCACACCG
TGCACTTCTC
CCTTTCCTTA
AAGAACCACC
ATCAACTTTA
GGAGGAGGGA
AAGGGGTGGC
TCTGGCAGGA
AAAGCCAGAA
TCCCCTCTAG
CCAGCAGAGA
GAGAGAGGAA
TGGCTGCATG
TTTTCTCCCC
CAGTCCAAGG
CACTGGGTCT
TGGCTGGGTT
GAAGGTTCCA
AGCTGCTCTC
CTGCTGTGTC
GGTGAGTTCT
GGTCAACCTG
CAACCTCCTG
ATATGGCCAT
TGCAGTTCAT
CGAGTCTTCA
GGGACTCCCC
ATGGCCTGGA
GTACTTTGCC
TTGCTTACAC
GGGAGAGGAG
AATGGATTTA
TAGAGAACAT
CATCTAAATC
CAACTTGACC
ATTGTGTGGC
CACACTTGCT
AGATTGCTAT
AGTCTAAATC
TAGCATTGTA
GAAAGACGGG
GGAGCTTGGA
GCTGCACAAA
CCCAGGTCTG
GAACTGGCTC
CTTACCTTGG
AAGGTGAATG
ATCCTGCCAG
GACTCTTAGC
CTCCCTGGGT
CTCAATTTCT
TTATCTGTTT
CATGGGAATG
AGGATCTCTG
CTGGGTGGTT
GGGTGATGTG
GGGGCTGTGT
GAAAACAGCT
TGTCAATACA
AGCCAAAATA
GAAATATTTC
TCCACAGAGT
ATGAAGGTCA
AATGAGAGAA
TACATTTAAA
TTAAATGGAA
AATTAAAATG
GTAAAAAATG
CAAAGCTGTA
TTGAAAGTTC
CGAGCTTCTC
TATAAGGAGC
TTTTTGACTA
TGTAAGAATC
CTGTACTCGT
TCCCCCTAAA
TATAAAAAAA
AGTTGAAGGA
GGCAGAAGGG
AGAGTGATGC
ACGATGGGCA
AGGACTTCAC
CTGCTGTTGC
TGGCTTTGAG
GATGGAGGAA
GGAGGCCACA
AACCTAGAAG
CTGGAGCCCC
TAGAAGCTAG
AAAAGGCAGG
GACCCGATTC
TTTCCTTGAG
CCTCCAGAAG
GGACACAGCC
CCGCCAGCAC
CTTGACTTTA
GCCTGGTGAG
ATCCTCTTAG
GACTTTTGGC
AATCAGAACT
ATAAGACAGA
AATGGAAGCC
ACTGAGTCTG
TAGCTGTTTG
TTGCAGCAGC
AATAGAAAAC
TAATGCAGGG
CCCAAGAAAT
CACTGGTGAT
GAGATCGGGA
AAGTGGGCTC
AGGAGGTCTG
GATCTGTGAT
GAGATGGGGA
AAGTGGGCTC
AAGAGGTCTG
GATCTGTGGT
GAGATGGGGG
AAGTGGGCTC
AGGAGGTCTG
GATCTGTGAT
GAGATGGGGA
AAGTGGGCTC
AGGAGGTCTG
GATCTGTGAT
GAGATGGGGA
AAGTGGGCTC
AGGAGGTCTG
GATCTGTGAT
GAGATGGGGG
AAGTGGGCTC
AGGAGGTCTG
GATCTGTGAT
GAGATGGGGG
AAGTGGGCTC
AGGAGGTCTG
GATCTGTGAT
GAGATGGGGG
AAGTGGGCTC
AGGAGGTCTG
GATCTGTGAT
GAGATGGGGA
AAGTGGGCTC
AGGAGGTCTG
GATCTGTGAT
GAGATGGGGG
AAGTGGGCTC
AGGAGGTCTG
GATCTGTGAT
GAGATGGGGA
AAGTGGGCTC
AGGAGGTCTG
GATCTGTGAT
GAGATCGGGA
AAGTGGGCTC
AAGAGGTCTG
GATCTGTGGT
GAGATGGGGG
AAGTGGGCTC
AGGAGGTCTG
GATCTGTGAT
GAGATGGGGA
AAGTGGGCTC
AGGAGGTCTG
GATCTGTGAT
GAGATGGGGA
AAGTGGGCTC
AGGAGGTCTG
GATCTGGGGT
GGGGATCTGG
AGTGGAAGGG
GAATTCATTT
GTTCATTGTC
TATCCTTTTG
TATTGATTGA
ATTTTTTATA
TATATATGTG
AATTTTCACA
ATAAAATTTT
TTTCCAAAAT
AAAATAAACA
AAAGGGGCTT
TTTGCAACCC
AATTCCTATC
TAAAAAAAAA
AAAAAAAAAA
AAAAGGATCC
GGGCCCGTTT
AAACCCGCTG
ATCAGCCTCG
ACTGTGCCTT
CTAGTTGCCA
GCCATCTGTT
GTTTGCCCCT
CCCCCGTGCC
TTCCTTGACC
CTGGAAGGTG
CCACTCCCAC
TGTCCTTTCC
TAATAAAATG
AGGAAATTGC
ATCGCATTGT
CTGAGTAGGT
GTCATTCTAT
TCTGGGGGGT
GGGGTGGGGC
AGGACAGCAA
GGGGGAGGAT
TGGGAAGACA
ATAGCAGGCA
TGCTGGGGAT
GCGGTGGGCT
CTATGGTCTA
GAGATATACT
GAGTCATTAG
GGACTTTCCA
ATGGGTTTTG
CCCAGTACAT
AAGGTCAATA
GGGGTGAATC
AACAGGAAAG
TCCCATTGGA
GCCAAGTACA
CTGAGTCAAT
AGGGACTTTC
CATTGGGTTT
TGCCCAGTAC
AAAAGGTCAA
TAGGGGGTGA
GTCAATGGGT
TTTTCCCATT
ATTGGCACGT
ACATAAGGTC
AATAGGGGTG
AGTCATTGGG
TTTTTCCAGC
CAATTTAATT
AAAACGCCAT
GTACTTTCCC
ACCATTGACG
TCAATGGGCT
ATTGAAACTA
ATGCAACGTG
ACCTTTAAAC
GGTACTTTCC
CATAGCTGAT
TAATGGGAAA
GTACCGTTCT
CGAGCCAATA
CACGTCAATG
GGAAGTGAAA
GGGCAGCCAA
AACGTAACAC
CGCCCCGGTT
TTCCCCTGGA
AATTCCATAT
TGGCACGCAT
TCTATTGGCT
GAGCTGCGTT
CTACGTGGGT
ATAAGAGGCG
CGACCAGCGT
CGGTACCGTC
GCAGTCTTCG
GTCTGACCAC
CGTAGAACGC
AGATCACCGG
TCGCCACCAT
GGTGAGCAAG
GGCGAGGAGC
TGTTCACCGG
GGTGGTGCCC
ATCCTGGTCG
AGCTGGACGG
CGACGTAAAC
GGCCACAAGT
TCAGCGTGTC
CGGCGAGGGC
GAGGGCGATG
CCACCTACGG
CAAGCTGACC
CTGAAGTTCA
TCTGCACCAC
CGGCAAGCTG
CCCGTGCCCT
GGCCCACCCT
CGTGACCACC
CTGACCTACG
GCGTGCAGTG
CTTCAGCCGC
TACCCCGACC
ACATGAAGCA
GCACGACTTC
TTCAAGTCCG
CCATGCCCGA
AGGCTACGTC
CAGGAGCGCA
CCATCTTCTT
CAAGGACGAC
GGCAACTACA
AGACCCGCGC
CGAGGTGAAG
TTCGAGGGCG
ACACCCTGGT
GAACCGCATC
GAGCTGAAGG
GCATCGACTT
CAAGGAGGAC
GGCAACATCC
TGGGGCACAA
GCTGGAGTAC
AACTACAACA
GCCACAACGT
CTATATCATG
GCCGACAAGC
AGAAGAACGG
CATCAAGGTG
AACTTCAAGA
TCCGCCACAA
CATCGAGGAC
GGCAGCGTGC
AGCTCGCCGA
CCACTACCAG
CAGAACACCC
CCATCGGCGA
CGGCCCCGTG
CTGCTGCCCG
ACAACCACTA
CCTGAGCACC
CAGTCCGCCC
TGAGCAAAGA
CCCCAACGAG
AAGCGCGATC
ACATGGTCCT
GCTGGAGTTC
GTGACCGCCG
CCGGGATCAC
TCTCGGCATG
GACGAGCTGT
ACAAGTAAAG
CGGCCGCGAC
TCTAGATCAT
AATCAGCCAT
ACCACATTTG
TAGAGGTTTT
ACTTGCTTTA
AAAAACCTCC
CACACCTCCC
CCTGAACCTG
AAACATAAAA
TGAATGCAAT
TGTTGTTGTT
AACTTGTTTA
TTGCAGCTTA
TAATGGTTAC
AAATAAAGCA
ATAGCATCAC
AAATTTCACA
AATAAAGCAT
TTTTTTCACT
GCATTCTAGT
TGTGGTTTGT
CCAAACTCAT
CAATGTATCT
TAAGGCGTAA
ATTGTAAGCG
TTAATATTTT
GTTAAAATTC
GCGTTAAATT
TTTGTTAAAT
CAGCTCATTT
TTTAACCAAT
AGGCCGAAAT
CGGCAAAATC
CCTTATAAAT
CAAAAGAATA
GACCGAGATA
GGGTTGAGTG
TTGTTCCAGT
TTGGAACAAG
AGTCCACTAT
TAAAGAACGT
GGACTCCAAC
GTCAAAGGGC
GAAAAACCGT
CTATCAGGGC
GATGGCCCAC
TACGTGAACC
ATCACCCTAA
TCAAGTTTTT
TGGGGTCGAG
GTGCCGTAAA
GCACTAAATC
GGAACCCTAA
AGGGAGCCCC
CGATTTAGAG
CTTGACGGGG
AAAGCCGGCG
AACGTGGCGA
GAAAGGAAGG
GAAGAAAGCG
AAAGGAGCGG
GCGCTAGGGC
GCTGGCAAGT
GTAGCGGTCA
CGCTGCGCGT
AACCACCACA
CCCGCCGCGC
TTAATGCGCC
GCTACAGGGC
GCGTCAGGTG
GCACTTTTCG
GGGAAATGTG
CGCGGAACCC
CTATTTGTTT
ATTTTTCTAA
ATACATTCAA
ATATGTATCC
GCTCATGAGA
CAATAACCCT
GATAAATGCT
TCAATAATAT
TGAAAAAGGA
AGAGTCCTGA
GGCGGAAAGA
ACCAGCTGTG
GAATGTGTGT
CAGTTAGGGT
GTGGAAAGTC
CCCAGGCTCC
CCAGCAGGCA
GAAGTATGCA
AAGCATGCAT
CTCAATTAGT
CAGCAACCAG
GTGTGGAAAG
TCCCCAGGCT
CCCCAGCAGG
CAGAAGTATG
CAAAGCATGC
ATCTCAATTA
GTCAGCAACC
ATAGTCCCGC
CCCTAACTCC
GCCCATCCCG
CCCCTAACTC
CGCCCAGTTC
CGCCCATTCT
CCGCCCCATG
GCTGACTAAT
TTTTTTTATT
TATGCAGAGG
CCGAGGCCGC
CTCGGCCTCT
GAGCTATTCC
AGAAGTAGTG
AGGAGGCTTT
TTTGGAGGCC
TAGGCTTTTG
CAAAGATCGA
TCAAGAGACA
GGATGAGGAT
CGTTTCGCAT
GATTGAACAA
GATGGATTGC
ACGCAGGTTC
TCCGGCCGCT
TGGGTGGAGA
GGCTATTCGG
CTATGACTGG
GCACAACAGA
CAATCGGCTG
CTCTGATGCC
GCCGTGTTCC
GGCTGTCAGC
GCAGGGGCGC
CCGGTTCTTT
TTGTCAAGAC
CGACCTGTCC
GGTGCCCTGA
ATGAACTGCA
AGACGAGGCA
GCGCGGCTAT
CGTGGCTGGC
CACGACGGGC
GTTCCTTGCG
CAGCTGTGCT
CGACGTTGTC
ACTGAAGCGG
GAAGGGACTG
GCTGCTATTG
GGCGAAGTGC
CGGGGCAGGA
TCTCCTGTCA
TCTCACCTTG
CTCCTGCCGA
GAAAGTATCC
ATCATGGCTG
ATGCAATGCG
GCGGCTGCAT
ACGCTTGATC
CGGCTACCTG
CCCATTCGAC
CACCAAGCGA
AACATCGCAT
CGAGCGAGCA
CGTACTCGGA
TGGAAGCCGG
TCTTGTCGAT
CAGGATGATC
TGGACGAAGA
GCATCAGGGG
CTCGCGCCAG
CCGAACTGTT
CGCCAGGCTC
AAGGCGAGCA
TGCCCGACGG
CGAGGATCTC
GTCGTGACCC
ATGGCGATGC
CTGCTTGCCG
AATATCATGG
TGGAAAATGG
CCGCTTTTCT
GGATTCATCG
ACTGTGGCCG
GCTGGGTGTG
GCGGACCGCT
ATCAGGACAT
AGCGTTGGCT
ACCCGTGATA
TTGCTGAAGA
GCTTGGCGGC
GAATGGGCTG
ACCGCTTCCT
CGTGCTTTAC
GGTATCGCCG
CTCCCGATTC
GCAGCGCATC
GCCTTCTATC
GCCTTCTTGA
CGAGTTCTTC
TGAGCGGGAC
TCTGGGGTTC
GAAATGACCG
ACCAAGCGAC
GCCCAACCTG
CCATCACGAG
ATTTCGATTC
CACCGCCGCC
TTCTATGAAA
GGTTGGGCTT
CGGAATCGTT
TTCCGGGACG
CCGGCTGGAT
GATCCTCCAG
CGCGGGGATC
TCATGCTGGA
GTTCTTCGCC
CACCCTAGGG
GGAGGCTAAC
TGAAACACGG
AAGGAGACAA
TACCGGAAGG
AACCCGCGCT
ATGACGGCAA
TAAAAAGACA
GAATAAAACG
CACGGTGTTG
GGTCGTTTGT
TCATAAACGC
GGGGTTCGGT
CCCAGGGCTG
GCACTCTGTC
GATACCCCAC
CGAGACCCCA
TTGGGGCCAA
TACGCCCGCG
TTTCTTCCTT
TTCCCCACCC
CACCCCCCAA
GTTCGGGTGA
AGGCCCAGGG
CTCGCAGCCA
ACGTCGGGGC
GGCAGGCCCT
GCCATAGCCT
CAGGTTACTC
ATATATACTT
TAGATTGATT
TAAAACTTCA
TTTTTAATTT
AAAAGGATCT
AGGTGAAGAT
CCTTTTTGAT
AATCTCATGA
CCAAAATCCC
TTAACGTGAG
TTTTCGTTCC
ACTGAGCGTC
AGACCCCGTA
GAAAAGATCA
AAGGATCTTC
TTGAGATCCT
TTTTTTCTGC
GCGTAATCTG
CTGCTTGCAA
ACAAAAAAAC
CACCGCTACC
AGCGGTGGTT
TGTTTGCCGG
ATCAAGAGCT
ACCAACTCTT
TTTCCGAAGG
TAACTGGCTT
CAGCAGAGCG
CAGATACCAA
ATACTGTCCT
TCTAGTGTAG
CCGTAGTTAG
GCCACCACTT
CAAGAACTCT
GTAGCACCGC
CTACATACCT
CGCTCTGCTA
ATCCTGTTAC
CAGTGGCTGC
TGCCAGTGGC
GATAAGTCGT
GTCTTACCGG
GTTGGACTCA
AGACGATAGT
TACCGGATAA
GGCGCAGCGG
TCGGGCTGAA
CGGGGGGTTC
GTGCACACAG
CCCAGCTTGG
AGCGAACGAC
CTACACCGAA
CTGAGATACC
TACAGCGTGA
GCTATGAGAA
AGCGCCACGC
TTCCCGAAGG
GAGAAAGGCG
GACAGGTATC
CGGTAAGCGG
CAGGGTCGGA
ACAGGAGAGC
GCACGAGGGA
GCTTCCAGGG
GGAAACGCCT
GGTATCTTTA
TAGTCCTGTC
GGGTTTCGCC
ACCTCTGACT
TGAGCGTCGA
TTTTTGTGAT
GCTCGTCAGG
GGGGCGGAGC
CTATGGAAAA
ACGCCAGCAA
CGCGGCCTTT
TTACGGTTCC
TGGCCTTTTG
CTGGCCTTTT
GCTCACATGT
TCTTTCCTGC
GTTATCCCCT
GATTCTGTGG
ATAACCGTAT
TACCGCCATG
CAT