名称: | pDESTTol2pA2_ubi:EGFP |
---|---|
别称: | Plasmid#27323 |
基因长: | |
质粒宿主: | 鱼类 |
质粒用途: | 启动子检测 |
片段类型: | Promoter |
片段物种: | 鱼 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | 绿色 |
启动子: | UBI |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TTATACATAG
TTGATAATTC
ACTGGCCGTC
GTTTTACGGT
ACCATCGATG
ATGATCCAGA
CATGATAAGA
TACATTGATG
AGTTTGGACA
AACCACAACT
AGAATGCAGT
GAAAAAAATG
CTTTATTTGT
GAAATTTGTG
ATGCTATTGC
TTTATTTGTA
ACCATTATAA
GCTGCAATAA
ACAAGTTAAC
AACAACAATT
GCATTCATTT
TATGTTTCAG
GTTCAGGGGG
AGGTGTGGGA
GGTTTTTTAA
AGCAAGTAAA
ACCTCTACAA
ATGTGGTATG
GCTGATTATG
ATCCTCTAGA
TCAGATCTGC
GAAGATACGG
CCACGGGTGC
TCTTGATCCT
GTGGCTGATT
TTGGACTGTG
CTGCTCGCAG
CTGCTGATGA
ATCACATACT
TCCTCCATTT
TCTTCCACTG
ATTGACTGTT
ATAATTTCCC
TAATTTCCAG
GTCAAGGTGC
TGTGCATTGT
GGTAATAGAT
GTGACATGAC
GTCACTTCCA
AAGGACCAAT
GAACATGTCT
GACCAATTTC
ATATAATGTG
AAAACGATTT
TCATAGGCAG
AATAAATAAC
ATTTAAATTA
AACTGGGCAT
CAGCGCAATT
CAATTGGTTT
GGTAATAGCA
AGGGAAAATA
GAATGAAGTG
ATCTCCAAAA
AATAAGTACT
TTTTGACTGT
AAATAAAATT
GTAAGGAGTA
AAAAGTACTT
TTTTTTCTAA
AAAAATGTAA
TTAAGTAAAA
GTAAAAGTAT
TGATTTTTAA
TTGTACTCAA
GTAAAGTAAA
AATCCCCAAA
AATAATACTT
AAGTACAGTA
ATCAAGTAAA
ATTACTCAAG
TACTTTACAC
CTCTGGTTCT
TGACCCCCTA
CCTTCAGCAA
GCCCAGCAGA
TCCACTAGTT
CTAGAGCGGC
CGCCACCGCG
GTGGAGCTCC
AGCTTTTGTT
CCCTTTAGTG
AGGGTTAATT
GCGCGCTTGG
CGTAATCATG
GTCATAGCTG
TTTCCTGTGT
GAAATTGTTA
TCCGCTCACA
ATTCCACACA
ACATACGAGC
CGGAAGCATA
AAGTGTAAAG
CCTGGGGTGC
CTAATGAGTG
AGCTAACTCA
CATTAATTGC
GTTGCGCTCA
CTGCCCGCTT
TCCAGTCGGG
AAACCTGTCG
TGCCAGCTGC
ATTAATGAAT
CGGCCAACGC
GCGGGGAGAG
GCGGTTTGCG
TATTGGGCGC
TCTTCCGCTT
CCTCGCTCAC
TGACTCGCTG
CGCTCGGTCG
TTCGGCTGCG
GCGAGCGGTA
TCAGCTCACT
CAAAGGCGGT
AATACGGTTA
TCCACAGAAT
CAGGGGATAA
CGCAGGAAAG
AACATGTGAG
CAAAAGGCCA
GCAAAAGGCC
AGGAACCGTA
AAAAGGCCGC
GTTGCTGGCG
TTTTTCCATA
GGCTCCGCCC
CCCTGACGAG
CATCACAAAA
ATCGACGCTC
AAGTCAGAGG
TGGCGAAACC
CGACAGGACT
ATAAAGATAC
CAGGCGTTTC
CCCCTGGAAG
CTCCCTCGTG
CGCTCTCCTG
TTCCGACCCT
GCCGCTTACC
GGATACCTGT
CCGCCTTTCT
CCCTTCGGGA
AGCGTGGCGC
TTTCTCATAG
CTCACGCTGT
AGGTATCTCA
GTTCGGTGTA
GGTCGTTCGC
TCCAAGCTGG
GCTGTGTGCA
CGAACCCCCC
GTTCAGCCCG
ACCGCTGCGC
CTTATCCGGT
AACTATCGTC
TTGAGTCCAA
CCCGGTAAGA
CACGACTTAT
CGCCACTGGC
AGCAGCCACT
GGTAACAGGA
TTAGCAGAGC
GAGGTATGTA
GGCGGTGCTA
CAGAGTTCTT
GAAGTGGTGG
CCTAACTACG
GCTACACTAG
AAGAACAGTA
TTTGGTATCT
GCGCTCTGCT
GAAGCCAGTT
ACCTTCGGAA
AAAGAGTTGG
TAGCTCTTGA
TCCGGCAAAC
AAACCACCGC
TGGTAGCGGT
GGTTTTTTTG
TTTGCAAGCA
GCAGATTACG
CGCAGAAAAA
AAGGATCTCA
AGAAGATCCT
TTGATCTTTT
CTACGGGGTC
TGACGCTCAG
TGGAACGAAA
ACTCACGTTA
AGGCATTTTG
GTCATGAGAT
TATCAAAAAG
GATCTTCACC
TAGATCCTTT
TAAATTAAAA
ATGAAGTTTT
AAATCAATCT
AAAGTATATA
TGAGTAAACT
TGGTCTGACA
GTTACCAATG
CTTAATCAGT
GAGGCACCTA
TCTCAGCGAT
CTGTCTATTT
CGTTCATCCA
TAGTTGCCTG
ACTCCCCGTC
GTGTAGATAA
CTACGATACG
GGAGGGCTTA
CCATCTGGCC
CCAGTGCTGC
AATGATACCG
CGAGACCCAC
GCTCACCGGC
TCCAGATTTA
TCAGCAATAA
ACCAGCCAGC
CGGAAGGGCC
GAGCGCAGAA
GTGGTCCTGC
AACTTTATCC
GCCTCCATCC
AGTCTATTAA
TTGTTGCCGG
GAAGCTAGAG
TAAGTAGTTC
GCCAGTTAAT
AGTTTGCGCA
ACGTTGTTGC
CATTGCTACA
GGCATCGTGG
TGTCACGCTC
GTCGTTTGGT
ATGGCTTCAT
TCAGCTCCGG
TTCCCAACGA
TCAAGGCGAG
TTACATGATC
CCCCATGTTG
TGCAAAAAAG
CGGTTAGCTC
CTTCGGTCCT
CCGATCGTTG
TCAGAAGTAA
GTTGGCCGCA
GTGTTATCAC
TCATGGTTAT
GGCAGCACTG
CATAATTCTC
TTACTGTCAT
GCCATCCGTA
AGATGCTTTT
CTGTGACTGG
TGAGTACTCA
ACCAAGTCAT
TCTGAGAATA
GTGTATGCGG
CGACCGAGTT
GCTCTTGCCC
GGCGTCAATA
CGGGATAATA
CCGCGCCACA
TAGCAGAACT
TTAAAAGTGC
TCATCATTGG
AAAACGTTCT
TCGGGGCGAA
AACTCTCAAG
GATCTTACCG
CTGTTGAGAT
CCAGTTCGAT
GTAACCCACT
CGTGCACCCA
ACTGATCTTC
AGCATCTTTT
ACTTTCACCA
GCGTTTCTGG
GTGAGCAAAA
ACAGGAAGGC
AAAATGCCGC
AAAAAAGGGA
ATAAGGGCGA
CACGGAAATG
TTGAATACTC
ATACTCTTCC
TTTTTCAATA
TTATTGAAGC
ATTTATCAGG
GTTATTGTCT
CATGAGCGGA
TACATATTTG
AATGTATTTA
GAAAAATAAA
CAAATAGGGG
TTCCGCGCAC
ATTTCCCCGA
AAAGTGCCAC
CTAAATTGTA
AGCGTTAATA
TTTTGTTAAA
ATTCGCGTTA
AATTTTTGTT
AAATCAGCTC
ATTTTTTAAC
CAATAGGCCG
AAATCGGCAA
AATCCCTTAT
AAATCAAAAG
AATAGACCGA
GATAGGGTTG
AGTGTTGTTC
CAGTTTGGAA
CAAGAGTCCA
CTATTAAAGA
ACGTGGACTC
CAACGTCAAA
GGGCGAAAAA
CCGTCTATCA
GGGCGATGGC
CCACGCTGGC
TAAGAACTCA
TCAGCCTCCC
CGGTCCATCT
ACCCACGTAC
CAATGCACCA
ATTGGCCACA
ATGACGGCTA
CTACATGGTG
CCATTCCTTC
CTCTTTATAG
GAATGGAGAC
TACCTCCTGT
CCAACAATGC
TCTTGGATAC
GAGTACGCCT
ACCTGTTGGA
CCCAGGTCAT
TGCACAACAC
CAGAAATGCC
CTCTGATCTG
CAAAAGACGT
GAATATCTGT
TCAGACACCC
ATATCCACTC
TGTTCCACAC
AGGTCAGAGG
TTTGTCCAGG
AGTTCTTGAC
AGAGGTGTAA
AAAGTACTCA
AAAATTTTAC
TCAAGTGAAA
GTACAAGTAC
TTAGGGAAAA
TTTTACTCAA
TTAAAAGTAA
AAGTATCTGG
CTAGAATCTT
ACTTGAGTAA
AAGTAAAAAA
GTACTCCATT
AAAATTGTAC
TTGAGTATTA
AGGAAGTAAA
AGTAAAAGCA
AGAAAGAAAA
CTAGAGATTC
TTGTTTAAGC
TTTTAATCTC
AAAAAACATT
AAATGAAATG
CATACAAGGT
TTTATCCTGC
TTTAGAACTG
TTTGTATTTA
ATTATCAAAC
TATAAGACAG
ACAATCTAAT
GCCAGTACAC
GCTACTCAAA
GTTGTAAAAC
CTCAGATTTA
ACTTCAGTAG
AAGCTGATTC
TCAAAATTGT
TAGTGTCAAG
CCTAGCTCTT
TTGGGGCTGA
AAAGCAATCC
TGCAGTGCTG
AAAAGCCTCT
CACAGGCAGC
CGATGCGGGA
AGAGGTGTAT
TAGTCTTGAT
AGAGAGGCTG
CAAATAGCAG
GAAACGTGAG
CAGAGACTCC
CTGGTGTCTG
AAACACAGGC
CAGATGGGCC
CTCGAGCAGG
AAACAGCTAT
GACCATGATT
ACGCCAAGCT
ATCAACTTTG
TATAGAAAAG
TTGGCTCCGA
ATTCGCCCTT
AAAACTCGAG
ACCAGCAAAG
TTCTAGAATT
TGTCGAAACA
TTTATGTTAT
ATATTTCCTG
AAAAAAATTC
TGAGTAAGTT
CTTAAGTGTT
ATTGCCAGCA
ACATAAACAA
CAGACGGCAA
AATGAATAAA
TGATAACAAA
GCAGTAGGCT
TAAATAAACC
TAATTTTTAT
AGGCTGTTCT
CTACAACCCT
CAAACAGTGA
TTAGTTTTGT
ACTTATAAAC
TTGCCCTTTC
ATTCATATTT
CAAGAAAATT
GGTTCAGAAG
ATCTGGATAT
TCTAGCAGTT
GTTCAAGCTC
ATGGAGGGAT
CAGTGACCTG
ATTCCACAAT
GACTAGGCCT
AATCCAGAAA
TTAGATGACT
GTCAACATAA
AAAGGCACAG
CACTCACTAG
CTGCCCTATA
TATTTTATTA
TATTTTACAT
ATATTATTTT
ATTTATTTAG
CTCTGAGTGC
TGTACTTTCT
GGTTAAAGAA
AACTGCTTAC
AACAGCTAAC
CTGTACTACC
TCAGGCTCAG
GGAATTTGGA
ACAGGTTTGT
CTGGTTTGTT
TCTTTAACCA
TGCATGCTTG
TTTTCAACTA
TGGCAACACA
GTCACATGGG
ACATTACAGA
AATGATTTGT
CGATGACATG
CGACTTTTCT
TTAATAAAGC
GCAAAGATCC
CAAAAAGCAA
ACTTTTAACA
AAAATCATAT
AATTATATTT
TCAATCCAGC
TTTGTAGCAA
CTTTGTGCTG
CTGTTCACTC
AGCAACAGAT
AGTCAGTATA
AGGTCAGTGT
GTCTCAAAGC
AGTGCCATCT
GTTTCACACA
TTGCGTTCTA
TATATAAGTG
TGCTGGTTGA
CACGACACTG
TATAAGGCCT
AGGCTAAAAC
ACAAACAATG
TAGAATGACA
CTGTGTTTTT
TTTGTAAACA
AATGTTGTTT
TTGGTTAAAC
ATCTTTGTGA
AAACATCCTC
CTGTCATGTA
TTTGCTATAT
TCAAATGTTA
AACCCGTGCA
GAATAGAACA
TATACAAAAA
AAAACAACAC
AACACATTTT
TAAACATTAT
TAAATATCAA
GTATTGCTGG
CAGTTCTGTT
TCTGTTTTAC
AGTACCCTTT
GCCACAGTTC
TCCGCTTTTC
CTGGTCCAGA
TTCCACAAGT
CTGATTCACC
AATAGCAAAG
CGAATAAACA
ACCAAAGCAG
CCAATCACTG
CTTGTAGACT
GTCCTGCGAG
ACCGGCCCAT
TCCAGCACAT
TCTGGAAACT
TCCTTTATAT
GATAATTATA
AATACATTTA
AATTATTGAT
ACAAAACATG
TAATTCCTAG
AACATAACCA
TAGCAATCAT
TAGTTTTCAG
GGTAATTATG
TATTTTTAGG
ATTTGACTGC
GGAAAGATCT
GGTCATGTGA
CGTCTCATGA
ACGTCACGGC
CCTGGGTTTC
TATAAATACA
GTAGGACTCT
CGACCATCGG
CAGATTTTTC
GAAGAAGAAG
ATCAGTTTCA
GGAGCCGTAC
TGTTCCGTTT
CAACGCAAAT
ATTAACGGTA
AGAGCGAATT
TCCTAGTTTG
TTTTCATGCC
ATTCTTTAAA
ACCATAGCGT
ATTACTTTAA
TTATAGTAAA
CTTTCGCTTT
CTTTATTACA
AGAGACGTTT
TGTGTTGATT
CTCCGCGGAC
ATTTTCGGTC
AGACAATCAG
AAAATGACCG
CGGAGGACCA
GTAACTTGCA
TTACACGTAA
GTTAAATCTT
CGTGTATTAA
AATGGTTAGG
TTGTTAACGT
CAAATAGGTT
ACCGTGTTTG
CGTGTGATCA
GGTTGGTTTT
GTTAGATTTT
TGTCAGTATT
TTTAATTTAT
TTGTTTTAGT
TTATTTATTT
TTTTTGCTGA
ATCATAGTTT
GTGAACAAAG
AACCCGGATG
TTACATACAG
TACAGCCGCC
ATGTTACAGA
GAGTTATAAC
TTAATCATTT
TAAAAATAAT
TTTGCCTTAC
TTTTAGTTTG
TCATGTTGAG
AAATGAGGAA
ATGTTAAAAT
GAGGAAATAT
CCAATTAATT
TAATATATCA
AAATAATCCA
TGATTACAAT
GCACTGAACT
GGAGAAAATT
AAGATGTTTT
CTAGTGTCAT
GAAACAAATG
TAAGAGATGT
ACATTGTAGA
TGTTTTATGT
CAAGAATTGG
CTAGTTGATG
CAGCATACTG
GCGATACTCA
GTTGTAATAA
CAGTAACGTT
ACATGTTAAT
AGACTACTGA
GTATGCTGTT
CTGTCTATGT
ATGCTCTGTA
AGCTACGAGA
AGGACTTTTT
TAAACAGTAA
AGGGTGCAAT
ATTTTTACAA
ATTGAATTAA
ATAAAGGCTG
TCTATTAAGT
AATATGCTTG
ATATTTTTCT
TACTTGATCG
GAAATAAGAA
AAAATATAAA
CGTTGTTGCT
CTAAAAATCC
TAGTTCAGTT
TAGCCAACCA
CAAATACCTT
TTTGTTCCTC
CAACAGTTTT
TTTTTCTTCT
CTATAATATT
TGGCAGTCTA
TAGTACTCCA
AATGTTTCCC
CACAGTCTAA
CTAATTGGTA
CAGCCAAAAT
CATGACACTT
ATTGCAATAA
TAATTTTGGT
TCATTGGCAT
TGTTGATAGC
CTGTGCCACT
AATATGGTCG
ATTGATCATG
CTTCAGGAAG
AAAACTATAT
TGTTTGATGT
AAGATTATTA
AATCTTCACC
TGCTTCCATT
ACAAACTATT
CCCATCTTAT
TGAATTCTGG
TATGTCTTAA
AGGATTAGTT
CACTTCCCAA
ATCAAAATTT
ACTTAGCCTT
TTTTCATCCA
TGATCCCTTT
TTTTCATCAT
TAATGAAGAA
ATTGTTTTTG
AAAAAGTTTC
AAGATTTTTT
TCTCTATATT
GTGGAGCTTG
TTAGTTTAAA
ATTCCAAAAT
GCAATATGTG
GCTTCAAATG
GTTCTAAATG
ATCCCAGTCA
AGGAATAACA
GTCTTATCTA
ATGAAACCAT
TAGACCTTTA
AAAATAAAAA
AAATAAAAGT
ATTTATTTTT
AAATGACTGA
GTGATTAAGT
TGAATTTCAG
CGTTTCCTTA
CTGTGTAGAA
GTCCTTCCTT
ACTGGCCCCA
CCCTTTGGTT
CTCTGCCAAT
CTGCTACCTA
ATGTAATGTT
GTGGAACATT
ATTATTCTTT
ATTTCTTAAT
TTTTTATTTT
TTATTTTAAA
ACAATGTAAA
CTGCACAGAT
GTGCAGTTTG
TTTAAAATGG
CCAATGCTTT
GGAAATGCAT
GACATAATTA
GATTTCATGA
TGCACAAAGC
CAAATCTCAG
AGCTTGTGCA
AAATGAGCTA
TCATTTCACT
AGGTAAGACC
CTAAATTTTC
ATATAGGATC
ATTTGGACAA
TTTTGCTGCA
GGTAAAATGC
ATTCTATAGT
CCACTGTCAG
CCATTGTTTT
GGATAGTATT
TATTTTTCTC
TACAAGTATA
GTCAATAGTT
TTCTATTATT
TTAAAGGTTT
GTAACATTTA
AGGGTGACCA
AATGCAAAGT
AAAATTTCAT
TTTCGGGTGA
ACTATCTCGT
TTAACATGGG
AGAAGTGCAA
AACATACATT
ATTGGCTAGA
ACATTGTAGT
ATTTTTTAAA
TGGAAATGTG
TGATTGCTAA
TCTTACTTTG
AATTTGTTTA
CAGGGATCCT
TTAAGGGCGA
ATTCGACCCA
AGTTTGTACA
AAAAAGCAGG
CTGGACCATG
GTGAGCAAGG
GCGAGGAGCT
GTTCACCGGG
GTGGTGCCCA
TCCTGGTCGA
GCTGGACGGC
GACGTAAACG
GCCACAAGTT
CAGCGTGTCC
GGCGAGGGCG
AGGGCGATGC
CACCTACGGC
AAGCTGACCC
TGAAGTTCAT
CTGCACCACC
GGCAAGCTGC
CCGTGCCCTG
GCCCACCCTC
GTGACCACCC
TGACCTACGG
CGTGCAGTGC
TTCAGCCGCT
ACCCCGACCA
CATGAAGCAG
CACGACTTCT
TCAAGTCCGC
CATGCCCGAA
GGCTACGTCC
AGGAGCGCAC
CATCTTCTTC
AAGGACGACG
GCAACTACAA
GACCCGCGCC
GAGGTGAAGT
TCGAGGGCGA
CACCCTGGTG
AACCGCATCG
AGCTGAAGGG
CATCGACTTC
AAGGAGGACG
GCAACATCCT
GGGGCACAAG
CTGGAGTACA
ACTACAACAG
CCACAACGTC
TATATCATGG
CCGACAAGCA
GAAGAACGGC
ATCAAGGTGA
ACTTCAAGAT
CCGCCACAAC
ATCGAGGACG
GCAGCGTGCA
GCTCGCCGAC
CACTACCAGC
AGAACACCCC
CATCGGCGAC
GGCCCCGTGC
TGCTGCCCGA
CAACCACTAC
CTGAGCACCC
AGTCCGCCCT
GAGCAAAGAC
CCCAACGAGA
AGCGCGATCA
CATGGTCCTG
CTGGAGTTCG
TGACCGCCGC
CGGGATCACT
CTCGGCATGG
ACGAGCTGTA
CAAGGGCGGT
GGAAGATCTG
GGAATTCAAG
GCCTCTCGAG
CCTCTAGATT
CTGCAGCCCT
ATAGCACCCA
GCTTTCTTGT
ACAAAGTGGG
GGATCCAGAC
ATGATAAGAT
ACATTGATGA
GTTTGGACAA
ACCACAACTA
GAATGCAGTG
AAAAAAATGC
TTTATTTGTG
AAATTTGTGA
TGCTATTGCT
TTATTTGTAA
CCATTATAAG
CTGCAATAAA
CAAGTTAACA
ACAACAATTG
CATTCATTTT
ATGTTTCAGG
TTCAGGGGGA
GGTGTGGGAG
GTTTTTTCCA
ACTTTA