名称: | pLV2-EF1a-SARS-CoV-2-N-2×Strep-3×FLAG-Puro |
---|---|
别称: | |
基因长: | 1257bp |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 新冠病毒 |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | |
启动子: | EF1a |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TGGAAGGGCT
AATTCACTCC
CAAAGAAGAC
AAGATATCCT
TGATCTGTGG
ATCTACCACA
CACAAGGCTA
CTTCCCTGAT
TAGCAGAACT
ACACACCAGG
GCCAGGGGTC
AGATATCCAC
TGACCTTTGG
ATGGTGCTAC
AAGCTAGTAC
CAGTTGAGCC
AGATAAGGTA
GAAGAGGCCA
ATAAAGGAGA
GAACACCAGC
TTGTTACACC
CTGTGAGCCT
GCATGGGATG
GATGACCCGG
AGAGAGAAGT
GTTAGAGTGG
AGGTTTGACA
GCCGCCTAGC
ATTTCATCAC
GTGGCCCGAG
AGCTGCATCC
GGAGTACTTC
AAGAACTGCT
GATATCGAGC
TTGCTACAAG
GGACTTTCCG
CTGGGGACTT
TCCAGGGAGG
CGTGGCCTGG
GCGGGACTGG
GGAGTGGCGA
GCCCTCAGAT
CCTGCATATA
AGCAGCTGCT
TTTTGCCTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTGGCG
CCCGAACAGG
GACTTGAAAG
CGAAAGGGAA
ACCAGAGGAG
CTCTCTCGAC
GCAGGACTCG
GCTTGCTGAA
GCGCGCACGG
CAAGAGGCGA
GGGGCGGCGA
CTGGTGAGTA
CGCCAAAAAT
TTTGACTAGC
GGAGGCTAGA
AGGAGAGAGA
TGGGTGCGAG
AGCGTCAGTA
TTAAGCGGGG
GAGAATTAGA
TCGCGATGGG
AAAAAATTCG
GTTAAGGCCA
GGGGGAAAGA
AAAAATATAA
ATTAAAACAT
ATAGTATGGG
CAAGCAGGGA
GCTAGAACGA
TTCGCAGTTA
ATCCTGGCCT
GTTAGAAACA
TCAGAAGGCT
GTAGACAAAT
ACTGGGACAG
CTACAACCAT
CCCTTCAGAC
AGGATCAGAA
GAACTTAGAT
CATTATATAA
TACAGTAGCA
ACCCTCTATT
GTGTGCATCA
AAGGATAGAG
ATAAAAGACA
CCAAGGAAGC
TTTAGACAAG
ATAGAGGAAG
AGCAAAACAA
AAGTAAGACC
ACCGCACAGC
AAGCGGCCGG
CCGCTGATCT
TCAGACCTGG
AGGAGGAGAT
ATGAGGGACA
ATTGGAGAAG
TGAATTATAT
AAATATAAAG
TAGTAAAAAT
TGAACCATTA
GGAGTAGCAC
CCACCAAGGC
AAAGAGAAGA
GTGGTGCAGA
GAGAAAAAAG
AGCAGTGGGA
ATAGGAGCTT
TGTTCCTTGG
GTTCTTGGGA
GCAGCAGGAA
GCACTATGGG
CGCAGCGTCA
ATGACGCTGA
CGGTACAGGC
CAGACAATTA
TTGTCTGGTA
TAGTGCAGCA
GCAGAACAAT
TTGCTGAGGG
CTATTGAGGC
GCAACAGCAT
CTGTTGCAAC
TCACAGTCTG
GGGCATCAAG
CAGCTCCAGG
CAAGAATCCT
GGCTGTGGAA
AGATACCTAA
AGGATCAACA
GCTCCTGGGG
ATTTGGGGTT
GCTCTGGAAA
ACTCATTTGC
ACCACTGCTG
TGCCTTGGAA
TGCTAGTTGG
AGTAATAAAT
CTCTGGAACA
GATTTGGAAT
CACACGACCT
GGATGGAGTG
GGACAGAGAA
ATTAACAATT
ACACAAGCTT
AATACACTCC
TTAATTGAAG
AATCGCAAAA
CCAGCAAGAA
AAGAATGAAC
AAGAATTATT
GGAATTAGAT
AAATGGGCAA
GTTTGTGGAA
TTGGTTTAAC
ATAACAAATT
GGCTGTGGTA
TATAAAATTA
TTCATAATGA
TAGTAGGAGG
CTTGGTAGGT
TTAAGAATAG
TTTTTGCTGT
ACTTTCTATA
GTGAATAGAG
TTAGGCAGGG
ATATTCACCA
TTATCGTTTC
AGACCCACCT
CCCAACCCCG
AGGGGACCCG
ACAGGCCCGA
AGGAATAGAA
GAAGAAGGTG
GAGAGAGAGA
CAGAGACAGA
TCCATTCGAT
TAGTGAACGG
ATCTCGACGG
TATCGCCTTT
AAAAGAAAAG
GGGGGATTGG
GGGGTACAGT
GCAGGGGAAA
GAATAGTAGA
CATAATAGCA
ACAGACATAC
AAACTAAAGA
ATTACAAAAA
CAAATTACAA
AAATTCAAAA
TTTTCGGGTT
TATTACAGGG
ACAGCAGAGA
TCCAGTTTAT
CGATGAGTAA
TTCATACAAA
AGGACTCGCC
CCTGCCTTGG
GGAATCCCAG
GGACCGTCGT
TAAACTCCCA
CTAACGTAGA
ACCCAGAGAT
CGCTGCGTTC
CCGCCCCCTC
ACCCGCCCGC
TCTCGTCATC
ACTGAGGTGG
AGAAGAGCAT
GCGTGAGGCT
CCGGTGCCCG
TCAGTGGGCA
GAGCGCACAT
CGCCCACAGT
CCCCGAGAAG
TTGGGGGGAG
GGGTCGGCAA
TTGAACCGGT
GCCTAGAGAA
GGTGGCGCGG
GGTAAACTGG
GAAAGTGATG
TCGTGTACTG
GCTCCGCCTT
TTTCCCGAGG
GTGGGGGAGA
ACCGTATATA
AGTGCAGTAG
TCGCCGTGAA
CGTTCTTTTT
CGCAACGGGT
TTGCCGCCAG
AACACAGGTA
AGTGCCGTGT
GTGGTTCCCG
CGGGCCTGGC
CTCTTTACGG
GTTATGGCCC
TTGCGTGCCT
TGAATTACTT
CCACGCCCCT
GGCTGCAGTA
CGTGATTCTT
GATCCCGAGC
TTCGGGTTGG
AAGTGGGTGG
GAGAGTTCGA
GGCCTTGCGC
TTAAGGAGCC
CCTTCGCCTC
GTGCTTGAGT
TGAGGCCTGG
CTTGGGCGCT
GGGGCCGCCG
CGTGCGAATC
TGGTGGCACC
TTCGCGCCTG
TCTCGCTGCT
TTCGATAAGT
CTCTAGCCAT
TTAAAATTTT
TGATGACCTG
CTGCGACGCT
TTTTTTCTGG
CAAGATAGTC
TTGTAAATGC
GGGCCAAGAT
CTGCACACTG
GTATTTCGGT
TTTTGGGGCC
GCGGGCGGCG
ACGGGGCCCG
TGCGTCCCAG
CGCACATGTT
CGGCGAGGCG
GGGCCTGCGA
GCGCGGCCAC
CGAGAATCGG
ACGGGGGTAG
TCTCAAGCTG
GCCGGCCTGC
TCTGGTGCCT
GGCCTCGCGC
CGCCGTGTAT
CGCCCCGCCC
TGGGCGGCAA
GGCTGGCCCG
GTCGGCACCA
GTTGCGTGAG
CGGAAAGATG
GCCGCTTCCC
GGCCCTGCTG
CAGGGAGCTC
AAAATGGAGG
ACGCGGCGCT
CGGGAGAGCG
GGCGGGTGAG
TCACCCACAC
AAAGGAAAAG
GGCCTTTCCG
TCCTCAGCCG
TCGCTTCATG
TGACTCCACG
GAGTACCGGG
CGCCGTCCAG
GCACCTCGAT
TAGTTCTCGA
GCTTTTGGAG
TACGTCGTCT
TTAGGTTGGG
GGGAGGGGTT
TTATGCGATG
GAGTTTCCCC
ACACTGAGTG
GGTGGAGACT
GAAGTTAGGC
CAGCTTGGCA
CTTGATGTAA
TTCTCCTTGG
AATTTGCCCT
TTTTGAGTTT
GGATCTTGGT
TCATTCTCAA
GCCTCAGACA
GTGGTTCAAA
GTTTTTTTCT
TCCATTTCAG
GTGTCGTGAG
GAATTAGCTT
GGTACGAATT
CGCCACCATG
AGCGATAACG
GCCCCCAAAA
CCAACGGAAT
GCCCCTCGGA
TAACATTTGG
TGGCCCTAGT
GATTCCACAG
GAAGTAACCA
AAACGGAGAA
CGGTCTGGAG
CCCGCTCTAA
ACAGAGGCGA
CCACAGGGAT
TGCCAAATAA
CACAGCGTCA
TGGTTTACAG
CCCTGACTCA
ACATGGAAAG
GAGGACTTGA
AATTTCCACG
AGGACAGGGT
GTACCAATAA
ACACAAACAG
TAGTCCAGAC
GATCAGATCG
GGTATTATAG
AAGGGCCACG
AGACGCATCA
GAGGTGGAGA
TGGTAAAATG
AAAGATCTCA
GTCCGCGCTG
GTACTTTTAT
TATTTGGGAA
CCGGCCCAGA
AGCGGGCCTC
CCATATGGAG
CCAATAAGGA
CGGGATTATA
TGGGTGGCTA
CAGAGGGAGC
CCTCAATACG
CCAAAAGATC
ACATCGGAAC
AAGAAACCCT
GCTAATAATG
CCGCAATCGT
GCTGCAGTTG
CCTCAGGGGA
CGACGCTTCC
TAAAGGCTTT
TACGCAGAAG
GATCACGCGG
CGGCAGCCAA
GCATCCTCAA
GGTCTAGTTC
CAGAAGTCGA
AACAGCTCCA
GAAACTCCAC
ACCAGGGTCC
AGTAGGGGCA
CAAGTCCGGC
GCGGATGGCG
GGCAACGGCG
GAGACGCCGC
ACTCGCTCTG
TTGCTCCTGG
ACCGGCTCAA
CCAACTTGAA
TCCAAGATGA
GTGGTAAGGG
ACAGCAGCAA
CAAGGTCAAA
CCGTAACCAA
GAAAAGCGCT
GCAGAAGCTT
CTAAAAAACC
TCGACAAAAA
CGGACCGCTA
CGAAGGCATA
TAACGTTACT
CAAGCCTTCG
GGAGACGAGG
GCCGGAGCAA
ACCCAGGGGA
ATTTTGGAGA
TCAGGAGCTC
ATCCGGCAAG
GGACAGATTA
CAAACATTGG
CCTCAAATCG
CTCAGTTCGC
GCCGTCCGCA
AGTGCTTTTT
TCGGAATGTC
TCGCATCGGT
ATGGAAGTAA
CACCATCCGG
GACATGGCTC
ACTTATACCG
GCGCCATCAA
GCTGGACGAC
AAAGATCCAA
ATTTCAAAGA
TCAGGTGATT
CTCTTGAATA
AACACATAGA
CGCGTATAAG
ACGTTTCCAC
CCACCGAACC
CAAGAAGGAC
AAAAAGAAAA
AAGCTGATGA
AACACAAGCG
TTGCCCCAGC
GACAGAAGAA
GCAGCAAACC
GTCACCCTCC
TGCCAGCCGC
TGATCTCGAT
GACTTCTCCA
AGCAACTTCA
ACAGTCCATG
AGCTCAGCCG
ATTCTACTCA
GGCGGGCCGC
CTCGAAGGCG
GCGGGGGATG
GAGCCATCCA
CAATTCGAGA
AAGGCGGTGG
TTCAGGAGGA
GGTAGCGGGG
GTGGATCATG
GTCACATCCG
CAGTTTGAAA
AGACTAGTGA
CTACAAAGAC
CATGACGGTG
ATTATAAAGA
TCATGACATC
GACTACAAGG
ATGACGATGA
CAAGTAGTCT
AGAGCGGCCG
CGGATCCCGC
CCCTCTCCCT
CCCCCCCCCC
TAACGTTACT
GGCCGAAGCC
GCTTGGAATA
AGGCCGGTGT
GCGTTTGTCT
ATATGTTATT
TTCCACCATA
TTGCCGTCTT
TTGGCAATGT
GAGGGCCCGG
AAACCTGGCC
CTGTCTTCTT
GACGAGCATT
CCTAGGGGTC
TTTCCCCTCT
CGCCAAAGGA
ATGCAAGGTC
TGTTGAATGT
CGTGAAGGAA
GCAGTTCCTC
TGGAAGCTTC
TTGAAGACAA
ACAACGTCTG
TAGCGACCCT
TTGCAGGCAG
CGGAACCCCC
CACCTGGCGA
CAGGTGCCTC
TGCGGCCAAA
AGCCACGTGT
ATAAGATACA
CCTGCAAAGG
CGGCACAACC
CCAGTGCCAC
GTTGTGAGTT
GGATAGTTGT
GGAAAGAGTC
AAATGGCTCT
CCTCAAGCGT
ATTCAACAAG
GGGCTGAAGG
ATGCCCAGAA
GGTACCCCAT
TGTATGGGAT
CTGATCTGGG
GCCTCGGTGC
ACATGCTTTA
CATGTGTTTA
GTCGAGGTTA
AAAAAACGTC
TAGGCCCCCC
GAACCACGGG
GACGTGGTTT
TCCTTTGAAA
AACACGATGA
TAAGCTTGCC
ACAACCCACA
AGGAGACGAC
CTTCCATGAC
CGAGTACAAG
CCCACGGTGC
GCCTCGCCAC
CCGCGACGAC
GTCCCCCGGG
CCGTACGCAC
CCTCGCCGCC
GCGTTCGCCG
ACTACCCCGC
CACGCGCCAC
ACCGTCGACC
CGGACCGCCA
CATCGAGCGG
GTCACCGAGC
TGCAAGAACT
CTTCCTCACG
CGCGTCGGGC
TCGACATCGG
CAAGGTGTGG
GTCGCGGACG
ACGGCGCCGC
GGTGGCGGTC
TGGACCACGC
CGGAGAGCGT
CGAAGCGGGG
GCGGTGTTCG
CCGAGATCGG
CCCGCGCATG
GCCGAGTTGA
GCGGTTCCCG
GCTGGCCGCG
CAGCAACAGA
TGGAAGGCCT
CCTGGCGCCG
CACCGGCCCA
AGGAGCCCGC
GTGGTTCCTG
GCCACCGTCG
GCGTCTCGCC
CGACCACCAG
GGCAAGGGTC
TGGGCAGCGC
CGTCGTGCTC
CCCGGAGTGG
AGGCGGCCGA
GCGCGCCGGG
GTGCCCGCCT
TCCTGGAGAC
CTCCGCGCCC
CGCAACCTCC
CCTTCTACGA
GCGGCTCGGC
TTCACCGTCA
CCGCCGACGT
CGAGGTGCCC
GAAGGACCGC
GCACCTGGTG
CATGACCCGC
AAGCCCGGTG
CCTAGACGCG
TCTGGAACAA
TCAACCTCTG
GATTACAAAA
TTTGTGAAAG
ATTGACTGGT
ATTCTTAACT
ATGTTGCTCC
TTTTACGCTA
TGTGGATACG
CTGCTTTAAT
GCCTTTGTAT
CATGCTATTG
CTTCCCGTAT
GGCTTTCATT
TTCTCCTCCT
TGTATAAATC
CTGGTTGCTG
TCTCTTTATG
AGGAGTTGTG
GCCCGTTGTC
AGGCAACGTG
GCGTGGTGTG
CACTGTGTTT
GCTGACGCAA
CCCCCACTGG
TTGGGGCATT
GCCACCACCT
GTCAGCTCCT
TTCCGGGACT
TTCGCTTTCC
CCCTCCCTAT
TGCCACGGCG
GAACTCATCG
CCGCCTGCCT
TGCCCGCTGC
TGGACAGGGG
CTCGGCTGTT
GGGCACTGAC
AATTCCGTGG
TGTTGTCGGG
GAAGCTGACG
TCCTTTCCAT
GGCTGCTCGC
CTGTGTTGCC
ACCTGGATTC
TGCGCGGGAC
GTCCTTCTGC
TACGTCCCTT
CGGCCCTCAA
TCCAGCGGAC
CTTCCTTCCC
GCGGCCTGCT
GCCGGCTCTG
CGGCCTCTTC
CGCGTCTTCG
CCTTCGCCCT
CAGACGAGTC
GGATCTCCCT
TTGGGCCGCC
TCCCCGCCTG
GAATTAATTC
TGCAGTCGAG
ACCTAGAAAA
ACATGGAGCA
ATCACAAGTA
GCAATACAGC
AGCTACCAAT
GCTGATTGTG
CCTGGCTAGA
AGCACAAGAG
GAGGAGGAGG
TGGGTTTTCC
AGTCACACCT
CAGGTACCTT
TAAGACCAAT
GACTTACAAG
GCAGCTGTAG
ATCTTAGCCA
CTTTTTAAAA
GAAAAGAGGG
GACTGGAAGG
GCTAATTCAC
TCCCAACGAA
GACAAGATAT
CCTTGATCTG
TGGATCTACC
ACACACAAGG
CTACTTCCCT
GATTAGCAGA
ACTACACACC
AGGGCCAGGG
GTCAGATATC
CACTGACCTT
TGGATGGTGC
TACAAGCTAG
TACCAGTTGA
GCCAGATAAG
GTAGAAGAGG
CCAATAAAGG
AGAGAACACC
AGCTTGTTAC
ACCCTGTGAG
CCTGCATGGG
ATGGATGACC
CGGAGAGAGA
AGTGTTAGAG
TGGAGGTTTG
ACAGCCGCCT
AGCATTTCAT
CACGTGGCCC
GAGAGCTGCA
TCCGGAGTAC
TTCAAGAACT
GCTGATATCG
AGCTTGCTAC
AAGGGACTTT
CCGCTGGGGA
CTTTCCAGGG
AGGCGTGGCC
TGGGCGGGAC
TGGGGAGTGG
CGAGCCCTCA
GATCCTGCAT
ATAAGCAGCT
GCTTTTTGCC
TGTACTGGGT
CTCTCTGGTT
AGACCAGATC
TGAGCCTGGG
AGCTCTCTGG
CTAACTAGGG
AACCCACTGC
TTAAGCCTCA
ATAAAGCTTG
CCTTGAGTGC
TTCAAGTAGT
GTGTGCCCGT
CTGTTGTGTG
ACTCTGGTAA
CTAGAGATCC
CTCAGACCCT
TTTAGTCAGT
GTGGAAAATC
TCTAGCAGTA
GTAGTTCATG
TCATCTTATT
ATTCAGTATT
TATAACTTGC
AAAGAAATGA
ATATCAGAGA
GTGAGAGGCC
TTGACATTGC
TAGCGTTTAC
CGTCGACCTC
TAGCTAGAGC
TTGGCGTAAT
CATGGTCATA
GCTGTTTCCT
GTGTGAAATT
GTTATCCGCT
CACAATTCCA
CACAACATAC
GAGCCGGAAG
CATAAAGTGT
AAAGCCTGGG
GTGCCTAATG
AGTGAGCTAA
CTCACATTAA
TTGCGTTGCG
CTCACTGCCC
GCTTTCCAGT
CGGGAAACCT
GTCGTGCCAG
CTGCATTAAT
GAATCGGCCA
ACGCGCGGGG
AGAGGCGGTT
TGCGTATTGG
GCGCTCTTCC
GCTTCCTCGC
TCACTGACTC
GCTGCGCTCG
GTCGTTCGGC
TGCGGCGAGC
GGTATCAGCT
CACTCAAAGG
CGGTAATACG
GTTATCCACA
GAATCAGGGG
ATAACGCAGG
AAAGAACATG
TGAGCAAAAG
GCCAGCAAAA
GGCCAGGAAC
CGTAAAAAGG
CCGCGTTGCT
GGCGTTTTTC
CATAGGCTCC
GCCCCCCTGA
CGAGCATCAC
AAAAATCGAC
GCTCAAGTCA
GAGGTGGCGA
AACCCGACAG
GACTATAAAG
ATACCAGGCG
TTTCCCCCTG
GAAGCTCCCT
CGTGCGCTCT
CCTGTTCCGA
CCCTGCCGCT
TACCGGATAC
CTGTCCGCCT
TTCTCCCTTC
GGGAAGCGTG
GCGCTTTCTC
ATAGCTCACG
CTGTAGGTAT
CTCAGTTCGG
TGTAGGTCGT
TCGCTCCAAG
CTGGGCTGTG
TGCACGAACC
CCCCGTTCAG
CCCGACCGCT
GCGCCTTATC
CGGTAACTAT
CGTCTTGAGT
CCAACCCGGT
AAGACACGAC
TTATCGCCAC
TGGCAGCAGC
CACTGGTAAC
AGGATTAGCA
GAGCGAGGTA
TGTAGGCGGT
GCTACAGAGT
TCTTGAAGTG
GTGGCCTAAC
TACGGCTACA
CTAGAAGAAC
AGTATTTGGT
ATCTGCGCTC
TGCTGAAGCC
AGTTACCTTC
GGAAAAAGAG
TTGGTAGCTC
TTGATCCGGC
AAACAAACCA
CCGCTGGTAG
CGGTGGTTTT
TTTGTTTGCA
AGCAGCAGAT
TACGCGCAGA
AAAAAAGGAT
CTCAAGAAGA
TCCTTTGATC
TTTTCTACGG
GGTCTGACGC
TCAGTGGAAC
GAAAACTCAC
GTTAAGGGAT
TTTGGTCATG
AGATTATCAA
AAAGGATCTT
CACCTAGATC
CTTTTAAATT
AAAAATGAAG
TTTTAAATCA
ATCTAAAGTA
TATATGAGTA
AACTTGGTCT
GACAGTTACC
AATGCTTAAT
CAGTGAGGCA
CCTATCTCAG
CGATCTGTCT
ATTTCGTTCA
TCCATAGTTG
CCTGACTCCC
CGTCGTGTAG
ATAACTACGA
TACGGGAGGG
CTTACCATCT
GGCCCCAGTG
CTGCAATGAT
ACCGCGAGAC
CCACGCTCAC
CGGCTCCAGA
TTTATCAGCA
ATAAACCAGC
CAGCCGGAAG
GGCCGAGCGC
AGAAGTGGTC
CTGCAACTTT
ATCCGCCTCC
ATCCAGTCTA
TTAATTGTTG
CCGGGAAGCT
AGAGTAAGTA
GTTCGCCAGT
TAATAGTTTG
CGCAACGTTG
TTGCCATTGC
TACAGGCATC
GTGGTGTCAC
GCTCGTCGTT
TGGTATGGCT
TCATTCAGCT
CCGGTTCCCA
ACGATCAAGG
CGAGTTACAT
GATCCCCCAT
GTTGTGCAAA
AAAGCGGTTA
GCTCCTTCGG
TCCTCCGATC
GTTGTCAGAA
GTAAGTTGGC
CGCAGTGTTA
TCACTCATGG
TTATGGCAGC
ACTGCATAAT
TCTCTTACTG
TCATGCCATC
CGTAAGATGC
TTTTCTGTGA
CTGGTGAGTA
CTCAACCAAG
TCATTCTGAG
AATAGTGTAT
GCGGCGACCG
AGTTGCTCTT
GCCCGGCGTC
AATACGGGAT
AATACCGCGC
CACATAGCAG
AACTTTAAAA
GTGCTCATCA
TTGGAAAACG
TTCTTCGGGG
CGAAAACTCT
CAAGGATCTT
ACCGCTGTTG
AGATCCAGTT
CGATGTAACC
CACTCGTGCA
CCCAACTGAT
CTTCAGCATC
TTTTACTTTC
ACCAGCGTTT
CTGGGTGAGC
AAAAACAGGA
AGGCAAAATG
CCGCAAAAAA
GGGAATAAGG
GCGACACGGA
AATGTTGAAT
ACTCATACTC
TTCCTTTTTC
AATATTATTG
AAGCATTTAT
CAGGGTTATT
GTCTCATGAG
CGGATACATA
TTTGAATGTA
TTTAGAAAAA
TAAACAAATA
GGGGTTCCGC
GCACATTTCC
CCGAAAAGTG
CCACCTGACG
TCGACGGATC
GGGAGATCAA
CTTGTTTATT
GCAGCTTATA
ATGGTTACAA
ATAAAGCAAT
AGCATCACAA
ATTTCACAAA
TAAAGCATTT
TTTTCACTGC
ATTCTAGTTG
TGGTTTGTCC
AAACTCATCA
ATGTATCTTA
TCATGTCTGG
ATCAACTGGA
TAACTCAAGC
TAACCAAAAT
CATCCCAAAC
TTCCCACCCC
ATACCCTATT
ACCACTGCCA
ATTACCTGTG
GTTTCATTTA
CTCTAAACCT
GTGATTCCTC
TGAATTATTT
TCATTTTAAA
GAAATTGTAT
TTGTTAAATA
TGTACTACAA
ACTTAGTAGT