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pCS2-6×Myc-NLRP14(human)

产品货号:P53441

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pCS2-6×Myc-NLRP14(human)
别称: NM_176822.4;CLR11.2; GC-LRR; NALP14; NOD5; PAN8
基因长: 3279bp
质粒宿主: 哺乳细胞,鱼类
质粒用途: 蛋白表达
片段类型: ORF
片段物种:
原核抗性: Amp
筛选标记:
荧光标记:
启动子: CMV
复制子: pUC
拷贝数:
感受态: Stbl3
温度: 30度
背景载体:
正向引物: CMV-F
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

CGCCATTCTG

CCTGGGGACG

TCGGAGCAAG

CTTGATTTAG

GTGACACTAT

AGAATACAAG

61

CTACTTGTTC

TTTTTGCAGG

ATCCCATCGA

TTTAAAGCTA

TGGAGCAAAA

GCTCATTTCT

121

GAAGAGGACT

TGAATGAAAT

GGAGCAAAAG

CTCATTTCTG

AAGAGGACTT

GAATGAAATG

181

GAGCAAAAGC

TCATTTCTGA

AGAGGACTTG

AATGAAATGG

AGCAAAAGCT

CATTTCTGAA

241

GAGGACTTGA

ATGAAATGGA

GCAAAAGCTC

ATTTCTGAAG

AGGACTTGAA

TGAAATGGAG

301

AGCTTGGGCG

ACCTCACCAT

GGAGCAAAAG

CTCATTTCTG

AAGAGGACTT

GAATTCAGCA

361

GATTCATCAT

CATCTTCTTT

CTTTCCTGAT

TTTGGGCTGC

TATTGTATTT

GGAGGAGCTA

421

AACAAAGAGG

AATTAAATAC

ATTCAAGTTA

TTCCTAAAGG

AGACCATGGA

ACCTGAGCAT

481

GGCCTGACAC

CCTGGAATGA

AGTGAAGAAG

GCCAGGCGGG

AGGACCTGGC

CAATTTGATG

541

AAGAAATATT

ATCCAGGAGA

GAAAGCCTGG

AGTGTGTCTC

TCAAAATCTT

TGGCAAGATG

601

AACCTGAAGG

ATCTGTGTGA

GAGAGCGAAA

GAAGAGATCA

ACTGGTCGGC

CCAGACTATA

661

GGACCAGATG

ATGCCAAGGC

TGGAGAGACA

CAAGAAGATC

AGGAGGCAGT

GCTGGGTGAT

721

GGAACAGAAT

ACAGAAATAG

AATAAAGGAA

AAATTTTGCA

TCACTTGGGA

CAAGAAGTCT

781

TTGGCTGGAA

AGCCTGAAGA

TTTCCATCAT

GGAATTGCAG

AGAAAGATAG

AAAACTGTTG

841

GAACACTTGT

TCGATGTGGA

TGTCAAAACC

GGTGCACAGC

CACAGATCGT

GGTGCTTCAG

901

GGAGCTGCTG

GAGTTGGGAA

AACAACCTTG

GTGAGAAAGG

CAATGTTAGA

TTGGGCAGAG

961

GGCAGTCTCT

ACCAGCAGAG

GTTTAAGTAT

GTTTTTTATC

TCAATGGGAG

AGAAATTAAC

1021

CAGCTGAAAG

AGAGAAGCTT

TGCTCAATTG

ATATCAAAGG

ACTGGCCCAG

CACAGAAGGC

1081

CCCATTGAAG

AAATCATGTA

CCAGCCAAGT

AGCCTCTTGT

TTATTATTGA

CAGTTTCGAT

1141

GAACTGAACT

TTGCCTTTGA

AGAACCTGAG

TTTGCACTGT

GCGAAGACTG

GACCCAAGAA

1201

CACCCAGTGT

CCTTCCTCAT

GAGTAGTTTG

CTGAGGAAAG

TGATGCTCCC

TGAGGCATCC

1261

TTATTGGTGA

CAACAAGACT

CACAACTTCT

AAGAGACTAA

AGCAGTTGTT

GAAGAATCAC

1321

CATTATGTAG

AGCTACTAGG

AATGTCTGAG

GATGCAAGAG

AGGAGTATAT

TTACCAGTTT

1381

TTTGAAGATA

AGAGGTGGGC

CATGAAAGTA

TTCAGTTCAC

TAAAAAGCAA

TGAGATGCTG

1441

TTTAGCATGT

GCCAAGTCCC

CCTAGTGTGC

TGGGCCGCTT

GTACTTGTCT

GAAGCAGCAA

1501

ATGGAGAAGG

GTGGTGATGT

CACATTGACC

TGCCAAACAA

CCACAGCTCT

GTTTACCTGC

1561

TATATTTCTA

GCTTGTTCAC

ACCAGTAGAT

GGAGGCTCTC

CTAGTCTACC

CAACCAAGCC

1621

CAGCTGAGAA

GACTGTGCCA

AGTCGCTGCC

AAAGGAATAT

GGACTATGAC

TTACGTGTTT

1681

TACAGAGAAA

ATCTCAGAAG

GCTTGGGTTA

ACTCAATCTG

ATGTCTCTAG

TTTTATGGAC

1741

AGCAATATTA

TTCAGAAGGA

CGCAGAGTAT

GAAAACTGCT

ATGTGTTCAC

CCACCTTCAT

1801

GTTCAGGAGT

TTTTTGCAGC

TATGTTCTAT

ATGTTGAAAG

GCAGTTGGGA

AGCTGGGAAC

1861

CCTTCCTGCC

AGCCTTTTGA

AGATTTGAAG

TCATTACTTC

AAAGCACAAG

TTATAAAGAC

1921

CCCCATTTGA

CACAGATGAA

GTGCTTTTTG

TTTGGCCTTT

TGAATGAAGA

TCGAGTAAAA

1981

CAACTGGAGA

GGACTTTTAA

CTGTAAAATG

TCACTGAAGA

TAAAATCAAA

GTTACTTCAG

2041

TGTATGGAAG

TATTAGGAAA

CAGTGACTAT

TCTCCATCAC

AGCTGGGATT

TCTGGAGTTG

2101

TTTCACTGTC

TGTATGAGAC

TCAAGATAAA

GCGTTTATAA

GCCAGGCAAT

GAGATGTTTC

2161

CCAAAGGTTG

CCATTAATAT

TTGTGAGAAA

ATACATTTGC

TTGTATCTTC

TTTCTGCCTT

2221

AAGCACTGCC

GGTGTTTGCG

GACCATCAGG

CTGTCTGTAA

CTGTGGTATT

TGAGAAGAAG

2281

ATATTAAAAA

CAAGCCTCCC

AACTAACACT

TGGGATGGTG

ATCGCATTAC

TCACTGTTGG

2341

CAAGATCTCT

GTTCTGTGCT

TCATACAAAT

GAACACTTGA

GAGAATTGGA

CCTGTACCAT

2401

AGCAACCTTG

ATAAATCAGC

AATGAATATC

CTGCATCATG

AACTAAGGCA

CCCAAACTGT

2461

AAACTACAAA

AGCTACTGTT

GAAATTTATC

ACTTTCCCTG

ATGGTTGTCA

GGATATCTCT

2521

ACTTCTTTGA

TTCATAACAA

GAATCTGATG

CATCTTGACC

TAAAAGGGAG

TGATATAGGG

2581

GATAATGGAG

TAAAGTCATT

GTGTGAGGCC

TTGAAACACC

CAGAGTGTAA

ACTACAGACT

2641

CTCAGGCTGG

AATCTTGCAA

CCTAACTGTA

TTTTGTTGTC

TAAATATATC

TAATGCTCTC

2701

ATCAGAAGCC

AGAGCCTGAT

ATTTCTGAAT

CTGTCAACCA

ATAATCTGTT

GGATGATGGA

2761

GTGCAGCTTT

TGTGTGAGGC

CTTAAGACAT

CCAAAGTGTT

ATCTAGAGAG

ACTGTCCTTA

2821

GAAAGCTGTG

GTCTCACAGA

GGCTGGCTGT

GAGTATCTTT

CTTTGGCTCT

CATCAGCAAT

2881

AAAAGACTGA

CACATTTGTG

CTTGGCAGAC

AATGTCTTGG

GTGATGGTGG

AGTAAAGCTT

2941

ATGAGTGATG

CCCTGCAACA

TGCACAATGT

ACTCTGAAGA

GCCTTGTGCT

GAGGCGTTGC

3001

CATTTCACTT

CACTTAGCAG

TGAATATCTG

TCAACTTCTC

TTCTACACAA

CAAGAGCCTG

3061

ACGCATCTGG

ATCTAGGATC

AAACTGGCTA

CAAGACAATG

GAGTGAAGCT

TCTGTGTGAT

3121

GTCTTTCGGC

ATCCAAGCTG

TAATCTTCAG

GACTTGGAAT

TGATGGGCTG

TGTTCTCACT

3181

AATGCATGTT

GTCTGGATCT

GGCTTCTGTT

ATTTTGAATA

ACCCAAACCT

GAGGAGCCTG

3241

GACCTTGGGA

ACAACGATTT

GCAGGATGAT

GGAGTGAAAA

TTCTGTGTGA

TGCTTTGAGA

3301

TATCCAAACT

GTAACATTCA

GAGGCTCGGG

TTGGAATACT

GTGGTTTGAC

ATCTCTCTGC

3361

TGTCAAGATC

TCTCCTCTGC

TCTTATCTGC

AACAAAAGAC

TGATAAAAAT

GAATCTGACA

3421

CAGAATACCT

TAGGATATGA

AGGAATTGTG

AAGTTATATA

AAGTCTTGAA

GTCTCCTAAG

3481

TGTAAACTAC

AAGTTCTAGG

GTTGTGCAAA

GAGGCATTTG

ATGAGGAAGC

CCAGAAGCTG

3541

CTGGAAGCTG

TGGGAGTTAG

CAATCCACAC

TTAATCATTA

AGCCAGATTG

TAACTATCAT

3601

AATGAAGAAG

ATGTGTCTTG

GTGGTGGTGT

TTCTGATCTA

GAACTATAGT

GAGTCGTATT

3661

ACGTAGATCC

AGACATGATA

AGATACATTG

ATGAGTTTGG

ACAAACCACA

ACTAGAATGC

3721

AGTGAAAAAA

ATGCTTTATT

TGTGAAATTT

GTGATGCTAT

TGCTTTATTT

GTAACCATTA

3781

TAAGCTGCAA

TAAACAAGTT

AACAACAACA

ATTGCATTCA

TTTTATGTTT

CAGGTTCAGG

3841

GGGAGGTGTG

GGAGGTTTTT

TAATTCGCGG

CCGCGGCGCC

AATGCATTGG

GCCCGGTACC

3901

CAGCTTTTGT

TCCCTTTAGT

GAGGGTTAAT

TGCGCGCTTG

GCGTAATCAT

GGTCATAGCT

3961

GTTTCCTGTG

TGAAATTGTT

ATCCGCTCAC

AATTCCACAC

AACATACGAG

CCGGAAGCAT

4021

AAAGTGTAAA

GCCTGGGGTG

CCTAATGAGT

GAGCTAACTC

ACATTAATTG

CGTTGCGCTC

4081

ACTGCCCGCT

TTCCAGTCGG

GAAACCTGTC

GTGCCAGCTG

CATTAATGAA

TCGGCCAACG

4141

CGCGGGGAGA

GGCGGTTTGC

GTATTGGGCG

CTCTTCCGCT

TCCTCGCTCA

CTGACTCGCT

4201

GCGCTCGGTC

GTTCGGCTGC

GGCGAGCGGT

ATCAGCTCAC

TCAAAGGCGG

TAATACGGTT

4261

ATCCACAGAA

TCAGGGGATA

ACGCAGGAAA

GAACATGTGA

GCAAAAGGCC

AGCAAAAGGC

4321

CAGGAACCGT

AAAAAGGCCG

CGTTGCTGGC

GTTTTTCCAT

AGGCTCCGCC

CCCCTGACGA

4381

GCATCACAAA

AATCGACGCT

CAAGTCAGAG

GTGGCGAAAC

CCGACAGGAC

TATAAAGATA

4441

CCAGGCGTTT

CCCCCTGGAA

GCTCCCTCGT

GCGCTCTCCT

GTTCCGACCC

TGCCGCTTAC

4501

CGGATACCTG

TCCGCCTTTC

TCCCTTCGGG

AAGCGTGGCG

CTTTCTCATA

GCTCACGCTG

4561

TAGGTATCTC

AGTTCGGTGT

AGGTCGTTCG

CTCCAAGCTG

GGCTGTGTGC

ACGAACCCCC

4621

CGTTCAGCCC

GACCGCTGCG

CCTTATCCGG

TAACTATCGT

CTTGAGTCCA

ACCCGGTAAG

4681

ACACGACTTA

TCGCCACTGG

CAGCAGCCAC

TGGTAACAGG

ATTAGCAGAG

CGAGGTATGT

4741

AGGCGGTGCT

ACAGAGTTCT

TGAAGTGGTG

GCCTAACTAC

GGCTACACTA

GAAGGACAGT

4801

ATTTGGTATC

TGCGCTCTGC

TGAAGCCAGT

TACCTTCGGA

AAAAGAGTTG

GTAGCTCTTG

4861

ATCCGGCAAA

CAAACCACCG

CTGGTAGCGG

TGGTTTTTTT

GTTTGCAAGC

AGCAGATTAC

4921

GCGCAGAAAA

AAAGGATCTC

AAGAAGATCC

TTTGATCTTT

TCTACGGGGT

CTGACGCTCA

4981

GTGGAACGAA

AACTCACGTT

AAGGGATTTT

GGTCATGAGA

TTATCAAAAA

GGATCTTCAC

5041

CTAGATCCTT

TTAAATTAAA

AATGAAGTTT

TAAATCAATC

TAAAGTATAT

ATGAGTAAAC

5101

TTGGTCTGAC

AGTTACCAAT

GCTTAATCAG

TGAGGCACCT

ATCTCAGCGA

TCTGTCTATT

5161

TCGTTCATCC

ATAGTTGCCT

GACTCCCCGT

CGTGTAGATA

ACTACGATAC

GGGAGGGCTT

5221

ACCATCTGGC

CCCAGTGCTG

CAATGATACC

GCGAGACCCA

CGCTCACCGG

CTCCAGATTT

5281

ATCAGCAATA

AACCAGCCAG

CCGGAAGGGC

CGAGCGCAGA

AGTGGTCCTG

CAACTTTATC

5341

CGCCTCCATC

CAGTCTATTA

ATTGTTGCCG

GGAAGCTAGA

GTAAGTAGTT

CGCCAGTTAA

5401

TAGTTTGCGC

AACGTTGTTG

CCATTGCTAC

AGGCATCGTG

GTGTCACGCT

CGTCGTTTGG

5461

TATGGCTTCA

TTCAGCTCCG

GTTCCCAACG

ATCAAGGCGA

GTTACATGAT

CCCCCATGTT

5521

GTGCAAAAAA

GCGGTTAGCT

CCTTCGGTCC

TCCGATCGTT

GTCAGAAGTA

AGTTGGCCGC

5581

AGTGTTATCA

CTCATGGTTA

TGGCAGCACT

GCATAATTCT

CTTACTGTCA

TGCCATCCGT

5641

AAGATGCTTT

TCTGTGACTG

GTGAGTACTC

AACCAAGTCA

TTCTGAGAAT

AGTGTATGCG

5701

GCGACCGAGT

TGCTCTTGCC

CGGCGTCAAT

ACGGGATAAT

ACCGCGCCAC

ATAGCAGAAC

5761

TTTAAAAGTG

CTCATCATTG

GAAAACGTTC

TTCGGGGCGA

AAACTCTCAA

GGATCTTACC

5821

GCTGTTGAGA

TCCAGTTCGA

TGTAACCCAC

TCGTGCACCC

AACTGATCTT

CAGCATCTTT

5881

TACTTTCACC

AGCGTTTCTG

GGTGAGCAAA

AACAGGAAGG

CAAAATGCCG

CAAAAAAGGG

5941

AATAAGGGCG

ACACGGAAAT

GTTGAATACT

CATACTCTTC

CTTTTTCAAT

ATTATTGAAG

6001

CATTTATCAG

GGTTATTGTC

TCATGAGCGG

ATACATATTT

GAATGTATTT

AGAAAAATAA

6061

ACAAATAGGG

GTTCCGCGCA

CATTTCCCCG

AAAAGTGCCA

CCTAAATTGT

AAGCGTTAAT

6121

ATTTTGTTAA

AATTCGCGTT

AAATTTTTGT

TAAATCAGCT

CATTTTTTAA

CCAATAGGCC

6181

GAAATCGGCA

AAATCCCTTA

TAAATCAAAA

GAATAGACCG

AGATAGGGTT

GAGTGTTGTT

6241

CCAGTTTGGA

ACAAGAGTCC

ACTATTAAAG

AACGTGGACT

CCAACGTCAA

AGGGCGAAAA

6301

ACCGTCTATC

AGGGCGATGG

CCCACTACGT

GAACCATCAC

CCTAATCAAG

TTTTTTGGGG

6361

TCGAGGTGCC

GTAAAGCACT

AAATCGGAAC

CCTAAAGGGA

GCCCCCGATT

TAGAGCTTGA

6421

CGGGGAAAGC

CGGCGAACGT

GGCGAGAAAG

GAAGGGAAGA

AAGCGAAAGG

AGCGGGCGCT

6481

AGGGCGCTGG

CAAGTGTAGC

GGTCACGCTG

CGCGTAACCA

CCACACCCGC

CGCGCTTAAT

6541

GCGCCGCTAC

AGGGCGCGTC

CCATTCGCCA

TTCAGGCTGC

GCAACTGTTG

GGAAGGGCGA

6601

TCGGTGCGGG

CCTCTTCGCT

ATTACGCCAG

TCGACCATAG

CCAATTCAAT

ATGGCGTATA

6661

TGGACTCATG

CCAATTCAAT

ATGGTGGATC

TGGACCTGTG

CCAATTCAAT

ATGGCGTATA

6721

TGGACTCGTG

CCAATTCAAT

ATGGTGGATC

TGGACCCCAG

CCAATTCAAT

ATGGCGGACT

6781

TGGCACCATG

CCAATTCAAT

ATGGCGGACT

TGGCACTGTG

CCAACTGGGG

AGGGGTCTAC

6841

TTGGCACGGT

GCCAAGTTTG

AGGAGGGGTC

TTGGCCCTGT

GCCAAGTCCG

CCATATTGAA

6901

TTGGCATGGT

GCCAATAATG

GCGGCCATAT

TGGCTATATG

CCAGGATCAA

TATATAGGCA

6961

ATATCCAATA

TGGCCCTATG

CCAATATGGC

TATTGGCCAG

GTTCAATACT

ATGTATTGGC

7021

CCTATGCCAT

ATAGTATTCC

ATATATGGGT

TTTCCTATTG

ACGTAGATAG

CCCCTCCCAA

7081

TGGGCGGTCC

CATATACCAT

ATATGGGGCT

TCCTAATACC

GCCCATAGCC

ACTCCCCCAT

7141

TGACGTCAAT

GGTCTCTATA

TATGGTCTTT

CCTATTGACG

TCATATGGGC

GGTCCTATTG

7201

ACGTATATGG

CGCCTCCCCC

ATTGACGTCA

ATTACGGTAA

ATGGCCCGCC

TGGCTCAATG

7261

CCCATTGACG

TCAATAGGAC

CACCCACCAT

TGACGTCAAT

GGGATGGCTC

ATTGCCCATT

7321

CATATCCGTT

CTCACGCCCC

CTATTGACGT

CAATGACGGT

AAATGGCCCA

CTTGGCAGTA

7381

CATCAATATC

TATTAATAGT

AACTTGGCAA

GTACATTACT

ATTGGAAGGA

CGCCAGGGTA

7441

CATTGGCAGT

ACTCCCATTG

ACGTCAATGG

CGGTAAATGG

CCCGCGATGG

CTGCCAAGTA

7501

CATCCCCATT

GACGTCAATG

GGGAGGGGCA

ATGACGCAAA

TGGGCGTTCC

ATTGACGTAA

7561

ATGGGCGGTA

GGCGTGCCTA

ATGGGAGGTC

TATATAAGCA

ATGCTCGTTT

AGGGAAC

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