名称: | pCS2-6×Myc-NLRP14(human) |
---|---|
别称: | NM_176822.4;CLR11.2; GC-LRR; NALP14; NOD5; PAN8 |
基因长: | 3279bp |
质粒宿主: | 哺乳细胞,鱼类 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 30度 |
背景载体: | |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CGCCATTCTG
CCTGGGGACG
TCGGAGCAAG
CTTGATTTAG
GTGACACTAT
AGAATACAAG
CTACTTGTTC
TTTTTGCAGG
ATCCCATCGA
TTTAAAGCTA
TGGAGCAAAA
GCTCATTTCT
GAAGAGGACT
TGAATGAAAT
GGAGCAAAAG
CTCATTTCTG
AAGAGGACTT
GAATGAAATG
GAGCAAAAGC
TCATTTCTGA
AGAGGACTTG
AATGAAATGG
AGCAAAAGCT
CATTTCTGAA
GAGGACTTGA
ATGAAATGGA
GCAAAAGCTC
ATTTCTGAAG
AGGACTTGAA
TGAAATGGAG
AGCTTGGGCG
ACCTCACCAT
GGAGCAAAAG
CTCATTTCTG
AAGAGGACTT
GAATTCAGCA
GATTCATCAT
CATCTTCTTT
CTTTCCTGAT
TTTGGGCTGC
TATTGTATTT
GGAGGAGCTA
AACAAAGAGG
AATTAAATAC
ATTCAAGTTA
TTCCTAAAGG
AGACCATGGA
ACCTGAGCAT
GGCCTGACAC
CCTGGAATGA
AGTGAAGAAG
GCCAGGCGGG
AGGACCTGGC
CAATTTGATG
AAGAAATATT
ATCCAGGAGA
GAAAGCCTGG
AGTGTGTCTC
TCAAAATCTT
TGGCAAGATG
AACCTGAAGG
ATCTGTGTGA
GAGAGCGAAA
GAAGAGATCA
ACTGGTCGGC
CCAGACTATA
GGACCAGATG
ATGCCAAGGC
TGGAGAGACA
CAAGAAGATC
AGGAGGCAGT
GCTGGGTGAT
GGAACAGAAT
ACAGAAATAG
AATAAAGGAA
AAATTTTGCA
TCACTTGGGA
CAAGAAGTCT
TTGGCTGGAA
AGCCTGAAGA
TTTCCATCAT
GGAATTGCAG
AGAAAGATAG
AAAACTGTTG
GAACACTTGT
TCGATGTGGA
TGTCAAAACC
GGTGCACAGC
CACAGATCGT
GGTGCTTCAG
GGAGCTGCTG
GAGTTGGGAA
AACAACCTTG
GTGAGAAAGG
CAATGTTAGA
TTGGGCAGAG
GGCAGTCTCT
ACCAGCAGAG
GTTTAAGTAT
GTTTTTTATC
TCAATGGGAG
AGAAATTAAC
CAGCTGAAAG
AGAGAAGCTT
TGCTCAATTG
ATATCAAAGG
ACTGGCCCAG
CACAGAAGGC
CCCATTGAAG
AAATCATGTA
CCAGCCAAGT
AGCCTCTTGT
TTATTATTGA
CAGTTTCGAT
GAACTGAACT
TTGCCTTTGA
AGAACCTGAG
TTTGCACTGT
GCGAAGACTG
GACCCAAGAA
CACCCAGTGT
CCTTCCTCAT
GAGTAGTTTG
CTGAGGAAAG
TGATGCTCCC
TGAGGCATCC
TTATTGGTGA
CAACAAGACT
CACAACTTCT
AAGAGACTAA
AGCAGTTGTT
GAAGAATCAC
CATTATGTAG
AGCTACTAGG
AATGTCTGAG
GATGCAAGAG
AGGAGTATAT
TTACCAGTTT
TTTGAAGATA
AGAGGTGGGC
CATGAAAGTA
TTCAGTTCAC
TAAAAAGCAA
TGAGATGCTG
TTTAGCATGT
GCCAAGTCCC
CCTAGTGTGC
TGGGCCGCTT
GTACTTGTCT
GAAGCAGCAA
ATGGAGAAGG
GTGGTGATGT
CACATTGACC
TGCCAAACAA
CCACAGCTCT
GTTTACCTGC
TATATTTCTA
GCTTGTTCAC
ACCAGTAGAT
GGAGGCTCTC
CTAGTCTACC
CAACCAAGCC
CAGCTGAGAA
GACTGTGCCA
AGTCGCTGCC
AAAGGAATAT
GGACTATGAC
TTACGTGTTT
TACAGAGAAA
ATCTCAGAAG
GCTTGGGTTA
ACTCAATCTG
ATGTCTCTAG
TTTTATGGAC
AGCAATATTA
TTCAGAAGGA
CGCAGAGTAT
GAAAACTGCT
ATGTGTTCAC
CCACCTTCAT
GTTCAGGAGT
TTTTTGCAGC
TATGTTCTAT
ATGTTGAAAG
GCAGTTGGGA
AGCTGGGAAC
CCTTCCTGCC
AGCCTTTTGA
AGATTTGAAG
TCATTACTTC
AAAGCACAAG
TTATAAAGAC
CCCCATTTGA
CACAGATGAA
GTGCTTTTTG
TTTGGCCTTT
TGAATGAAGA
TCGAGTAAAA
CAACTGGAGA
GGACTTTTAA
CTGTAAAATG
TCACTGAAGA
TAAAATCAAA
GTTACTTCAG
TGTATGGAAG
TATTAGGAAA
CAGTGACTAT
TCTCCATCAC
AGCTGGGATT
TCTGGAGTTG
TTTCACTGTC
TGTATGAGAC
TCAAGATAAA
GCGTTTATAA
GCCAGGCAAT
GAGATGTTTC
CCAAAGGTTG
CCATTAATAT
TTGTGAGAAA
ATACATTTGC
TTGTATCTTC
TTTCTGCCTT
AAGCACTGCC
GGTGTTTGCG
GACCATCAGG
CTGTCTGTAA
CTGTGGTATT
TGAGAAGAAG
ATATTAAAAA
CAAGCCTCCC
AACTAACACT
TGGGATGGTG
ATCGCATTAC
TCACTGTTGG
CAAGATCTCT
GTTCTGTGCT
TCATACAAAT
GAACACTTGA
GAGAATTGGA
CCTGTACCAT
AGCAACCTTG
ATAAATCAGC
AATGAATATC
CTGCATCATG
AACTAAGGCA
CCCAAACTGT
AAACTACAAA
AGCTACTGTT
GAAATTTATC
ACTTTCCCTG
ATGGTTGTCA
GGATATCTCT
ACTTCTTTGA
TTCATAACAA
GAATCTGATG
CATCTTGACC
TAAAAGGGAG
TGATATAGGG
GATAATGGAG
TAAAGTCATT
GTGTGAGGCC
TTGAAACACC
CAGAGTGTAA
ACTACAGACT
CTCAGGCTGG
AATCTTGCAA
CCTAACTGTA
TTTTGTTGTC
TAAATATATC
TAATGCTCTC
ATCAGAAGCC
AGAGCCTGAT
ATTTCTGAAT
CTGTCAACCA
ATAATCTGTT
GGATGATGGA
GTGCAGCTTT
TGTGTGAGGC
CTTAAGACAT
CCAAAGTGTT
ATCTAGAGAG
ACTGTCCTTA
GAAAGCTGTG
GTCTCACAGA
GGCTGGCTGT
GAGTATCTTT
CTTTGGCTCT
CATCAGCAAT
AAAAGACTGA
CACATTTGTG
CTTGGCAGAC
AATGTCTTGG
GTGATGGTGG
AGTAAAGCTT
ATGAGTGATG
CCCTGCAACA
TGCACAATGT
ACTCTGAAGA
GCCTTGTGCT
GAGGCGTTGC
CATTTCACTT
CACTTAGCAG
TGAATATCTG
TCAACTTCTC
TTCTACACAA
CAAGAGCCTG
ACGCATCTGG
ATCTAGGATC
AAACTGGCTA
CAAGACAATG
GAGTGAAGCT
TCTGTGTGAT
GTCTTTCGGC
ATCCAAGCTG
TAATCTTCAG
GACTTGGAAT
TGATGGGCTG
TGTTCTCACT
AATGCATGTT
GTCTGGATCT
GGCTTCTGTT
ATTTTGAATA
ACCCAAACCT
GAGGAGCCTG
GACCTTGGGA
ACAACGATTT
GCAGGATGAT
GGAGTGAAAA
TTCTGTGTGA
TGCTTTGAGA
TATCCAAACT
GTAACATTCA
GAGGCTCGGG
TTGGAATACT
GTGGTTTGAC
ATCTCTCTGC
TGTCAAGATC
TCTCCTCTGC
TCTTATCTGC
AACAAAAGAC
TGATAAAAAT
GAATCTGACA
CAGAATACCT
TAGGATATGA
AGGAATTGTG
AAGTTATATA
AAGTCTTGAA
GTCTCCTAAG
TGTAAACTAC
AAGTTCTAGG
GTTGTGCAAA
GAGGCATTTG
ATGAGGAAGC
CCAGAAGCTG
CTGGAAGCTG
TGGGAGTTAG
CAATCCACAC
TTAATCATTA
AGCCAGATTG
TAACTATCAT
AATGAAGAAG
ATGTGTCTTG
GTGGTGGTGT
TTCTGATCTA
GAACTATAGT
GAGTCGTATT
ACGTAGATCC
AGACATGATA
AGATACATTG
ATGAGTTTGG
ACAAACCACA
ACTAGAATGC
AGTGAAAAAA
ATGCTTTATT
TGTGAAATTT
GTGATGCTAT
TGCTTTATTT
GTAACCATTA
TAAGCTGCAA
TAAACAAGTT
AACAACAACA
ATTGCATTCA
TTTTATGTTT
CAGGTTCAGG
GGGAGGTGTG
GGAGGTTTTT
TAATTCGCGG
CCGCGGCGCC
AATGCATTGG
GCCCGGTACC
CAGCTTTTGT
TCCCTTTAGT
GAGGGTTAAT
TGCGCGCTTG
GCGTAATCAT
GGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGGACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGAGACCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATTGCTAC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAT
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATACTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTTCCGCGCA
CATTTCCCCG
AAAAGTGCCA
CCTAAATTGT
AAGCGTTAAT
ATTTTGTTAA
AATTCGCGTT
AAATTTTTGT
TAAATCAGCT
CATTTTTTAA
CCAATAGGCC
GAAATCGGCA
AAATCCCTTA
TAAATCAAAA
GAATAGACCG
AGATAGGGTT
GAGTGTTGTT
CCAGTTTGGA
ACAAGAGTCC
ACTATTAAAG
AACGTGGACT
CCAACGTCAA
AGGGCGAAAA
ACCGTCTATC
AGGGCGATGG
CCCACTACGT
GAACCATCAC
CCTAATCAAG
TTTTTTGGGG
TCGAGGTGCC
GTAAAGCACT
AAATCGGAAC
CCTAAAGGGA
GCCCCCGATT
TAGAGCTTGA
CGGGGAAAGC
CGGCGAACGT
GGCGAGAAAG
GAAGGGAAGA
AAGCGAAAGG
AGCGGGCGCT
AGGGCGCTGG
CAAGTGTAGC
GGTCACGCTG
CGCGTAACCA
CCACACCCGC
CGCGCTTAAT
GCGCCGCTAC
AGGGCGCGTC
CCATTCGCCA
TTCAGGCTGC
GCAACTGTTG
GGAAGGGCGA
TCGGTGCGGG
CCTCTTCGCT
ATTACGCCAG
TCGACCATAG
CCAATTCAAT
ATGGCGTATA
TGGACTCATG
CCAATTCAAT
ATGGTGGATC
TGGACCTGTG
CCAATTCAAT
ATGGCGTATA
TGGACTCGTG
CCAATTCAAT
ATGGTGGATC
TGGACCCCAG
CCAATTCAAT
ATGGCGGACT
TGGCACCATG
CCAATTCAAT
ATGGCGGACT
TGGCACTGTG
CCAACTGGGG
AGGGGTCTAC
TTGGCACGGT
GCCAAGTTTG
AGGAGGGGTC
TTGGCCCTGT
GCCAAGTCCG
CCATATTGAA
TTGGCATGGT
GCCAATAATG
GCGGCCATAT
TGGCTATATG
CCAGGATCAA
TATATAGGCA
ATATCCAATA
TGGCCCTATG
CCAATATGGC
TATTGGCCAG
GTTCAATACT
ATGTATTGGC
CCTATGCCAT
ATAGTATTCC
ATATATGGGT
TTTCCTATTG
ACGTAGATAG
CCCCTCCCAA
TGGGCGGTCC
CATATACCAT
ATATGGGGCT
TCCTAATACC
GCCCATAGCC
ACTCCCCCAT
TGACGTCAAT
GGTCTCTATA
TATGGTCTTT
CCTATTGACG
TCATATGGGC
GGTCCTATTG
ACGTATATGG
CGCCTCCCCC
ATTGACGTCA
ATTACGGTAA
ATGGCCCGCC
TGGCTCAATG
CCCATTGACG
TCAATAGGAC
CACCCACCAT
TGACGTCAAT
GGGATGGCTC
ATTGCCCATT
CATATCCGTT
CTCACGCCCC
CTATTGACGT
CAATGACGGT
AAATGGCCCA
CTTGGCAGTA
CATCAATATC
TATTAATAGT
AACTTGGCAA
GTACATTACT
ATTGGAAGGA
CGCCAGGGTA
CATTGGCAGT
ACTCCCATTG
ACGTCAATGG
CGGTAAATGG
CCCGCGATGG
CTGCCAAGTA
CATCCCCATT
GACGTCAATG
GGGAGGGGCA
ATGACGCAAA
TGGGCGTTCC
ATTGACGTAA
ATGGGCGGTA
GGCGTGCCTA
ATGGGAGGTC
TATATAAGCA
ATGCTCGTTT
AGGGAAC