名称: | pLenti6.2-ccdB-3xFLAG-V5 |
---|---|
别称: | Plasmid#87071 |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达 |
片段类型: | ORF,GateWay |
片段物种: | 空载体 |
原核抗性: | Amp,Chl |
筛选标记: | Blast |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DB3.1 |
温度: | 30度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GCCCCGCAGC
TCGCGTCGTG
CAGGACGTGA
CAAATGGAAG
TAGCACGTCT
CACTAGTCTC
GTGCAGATGG
ACAGCACCGC
TGAGCAATGG
AAGCGGGTAG
GCCTTTGGGG
CAGCGGCCAA
TAGCAGCTTT
GCTCCTTCGC
TTTCTGGGCT
CAGAGGCTGG
GAAGGGGTGG
GTCCGGGGGC
GGGCTCAGGG
GCGGGCTCAG
GGGCGGGGCG
GGCGCCCGAA
GGTCCTCCGG
AGGCCCGGCA
TTCTGCACGC
TTCAAAAGCG
CACGTCTGCC
GCGCTGTTCT
CCTCTTCCTC
ATCTCCGGGC
CTTTCGACTC
TAGACACGTG
TTGACAATTA
ATCATCGGCA
TAGTATATCG
GCATAGTATA
ATACGACAAG
GTGAGGAACT
AAACCATGGC
CAAGCCTTTG
TCTCAAGAAG
AATCCACCCT
CATTGAAAGA
GCAACGGCTA
CAATCAACAG
CATCCCCATC
TCTGAAGACT
ACAGCGTCGC
CAGCGCAGCT
CTCTCTAGCG
ACGGCCGCAT
CTTCACTGGT
GTCAATGTAT
ATCATTTTAC
TGGGGGACCT
TGTGCAGAAC
TCGTGGTGCT
GGGCACTGCT
GCTGCTGCGG
CAGCTGGCAA
CCTGACTTGT
ATCGTCGCGA
TCGGAAATGA
GAACAGGGGC
ATCTTGAGCC
CCTGCGGACG
GTGCCGACAG
GTGCTTCTCG
ATCTGCATCC
TGGGATCAAA
GCCATAGTGA
AGGACAGTGA
TGGACAGCCG
ACGGCAGTTG
GGATTCGTGA
ATTGCTGCCC
TCTGGTTATG
TGTGGGAGGG
CTAAGCACAA
TTCGAGCTCG
GTACCTTTAA
GACCAATGAC
TTACAAGGCA
GCTGTAGATC
TTAGCCACTT
TTTAAAAGAA
AAGGGGGGAC
TGGAAGGGCT
AATTCACTCC
CAACGAAGAC
AAGATCTGCT
TTTTGCTTGT
ACTGGGTCTC
TCTGGTTAGA
CCAGATCTGA
GCCTGGGAGC
TCTCTGGCTA
ACTAGGGAAC
CCACTGCTTA
AGCCTCAATA
AAGCTTGCCT
TGAGTGCTTC
AAGTAGTGTG
TGCCCGTCTG
TTGTGTGACT
CTGGTAACTA
GAGATCCCTC
AGACCCTTTT
AGTCAGTGTG
GAAAATCTCT
AGCAGTAGTA
GTTCATGTCA
TCTTATTATT
CAGTATTTAT
AACTTGCAAA
GAAATGAATA
TCAGAGAGTG
AGAGGAACTT
GTTTATTGCA
GCTTATAATG
GTTACAAATA
AAGCAATAGC
ATCACAAATT
TCACAAATAA
AGCATTTTTT
TCACTGCATT
CTAGTTGTGG
TTTGTCCAAA
CTCATCAATG
TATCTTATCA
TGTCTGGCTC
TAGCTATCCC
GCCCCTAACT
CCGCCCATCC
CGCCCCTAAC
TCCGCCCAGT
TCCGCCCATT
CTCCGCCCCA
TGGCTGACTA
ATTTTTTTTA
TTTATGCAGA
GGCCGAGGCC
GCCTCGGCCT
CTGAGCTATT
CCAGAAGTAG
TGAGGAGGCT
TTTTTGGAGG
CCTAGGGACG
TACCCAATTC
GCCCTATAGT
GAGTCGTATT
ACGCGCGCTC
ACTGGCCGTC
GTTTTACAAC
GTCGTGACTG
GGAAAACCCT
GGCGTTACCC
AACTTAATCG
CCTTGCAGCA
CATCCCCCTT
TCGCCAGCTG
GCGTAATAGC
GAAGAGGCCC
GCACCGATCG
CCCTTCCCAA
CAGTTGCGCA
GCCTGAATGG
CGAATGGGAC
GCGCCCTGTA
GCGGCGCATT
AAGCGCGGCG
GGTGTGGTGG
TTACGCGCAG
CGTGACCGCT
ACACTTGCCA
GCGCCCTAGC
GCCCGCTCCT
TTCGCTTTCT
TCCCTTCCTT
TCTCGCCACG
TTCGCCGGCT
TTCCCCGTCA
AGCTCTAAAT
CGGGGGCTCC
CTTTAGGGTT
CCGATTTAGT
GCTTTACGGC
ACCTCGACCC
CAAAAAACTT
GATTAGGGTG
ATGGTTCACG
TAGTGGGCCA
TCGCCCTGAT
AGACGGTTTT
TCGCCCTTTG
ACGTTGGAGT
CCACGTTCTT
TAATAGTGGA
CTCTTGTTCC
AAACTGGAAC
AACACTCAAC
CCTATCTCGG
TCTATTCTTT
TGATTTATAA
GGGATTTTGC
CGATTTCGGC
CTATTGGTTA
AAAAATGAGC
TGATTTAACA
AAAATTTAAC
GCGAATTTTA
ACAAAATATT
AACGCTTACA
ATTTAGGTGG
CACTTTTCGG
GGAAATGTGC
GCGGAACCCC
TATTTGTTTA
TTTTTCTAAA
TACATTCAAA
TATGTATCCG
CTCATGAGAC
AATAACCCTG
ATAAATGCTT
CAATAATATT
GAAAAAGGAA
GAGTATGAGT
ATTCAACATT
TCCGTGTCGC
CCTTATTCCC
TTTTTTGCGG
CATTTTGCCT
TCCTGTTTTT
GCTCACCCAG
AAACGCTGGT
GAAAGTAAAA
GATGCTGAAG
ATCAGTTGGG
TGCACGAGTG
GGTTACATCG
AACTGGATCT
CAACAGCGGT
AAGATCCTTG
AGAGTTTTCG
CCCCGAAGAA
CGTTTTCCAA
TGATGAGCAC
TTTTAAAGTT
CTGCTATGTG
GCGCGGTATT
ATCCCGTATT
GACGCCGGGC
AAGAGCAACT
CGGTCGCCGC
ATACACTATT
CTCAGAATGA
CTTGGTTGAG
TACTCACCAG
TCACAGAAAA
GCATCTTACG
GATGGCATGA
CAGTAAGAGA
ATTATGCAGT
GCTGCCATAA
CCATGAGTGA
TAACACTGCG
GCCAACTTAC
TTCTGACAAC
GATCGGAGGA
CCGAAGGAGC
TAACCGCTTT
TTTGCACAAC
ATGGGGGATC
ATGTAACTCG
CCTTGATCGT
TGGGAACCGG
AGCTGAATGA
AGCCATACCA
AACGACGAGC
GTGACACCAC
GATGCCTGTA
GCAATGGCAA
CAACGTTGCG
CAAACTATTA
ACTGGCGAAC
TACTTACTCT
AGCTTCCCGG
CAACAATTAA
TAGACTGGAT
GGAGGCGGAT
AAAGTTGCAG
GACCACTTCT
GCGCTCGGCC
CTTCCGGCTG
GCTGGTTTAT
TGCTGATAAA
TCTGGAGCCG
GTGAGCGTGG
GTCTCGCGGT
ATCATTGCAG
CACTGGGGCC
AGATGGTAAG
CCCTCCCGTA
TCGTAGTTAT
CTACACGACG
GGGAGTCAGG
CAACTATGGA
TGAACGAAAT
AGACAGATCG
CTGAGATAGG
TGCCTCACTG
ATTAAGCATT
GGTAACTGTC
AGACCAAGTT
TACTCATATA
TACTTTAGAT
TGATTTAAAA
CTTCATTTTT
AATTTAAAAG
GATCTAGGTG
AAGATCCTTT
TTGATAATCT
CATGACCAAA
ATCCCTTAAC
GTGAGTTTTC
GTTCCACTGA
GCGTCAGACC
CCGTAGAAAA
GATCAAAGGA
TCTTCTTGAG
ATCCTTTTTT
TCTGCGCGTA
ATCTGCTGCT
TGCAAACAAA
AAAACCACCG
CTACCAGCGG
TGGTTTGTTT
GCCGGATCAA
GAGCTACCAA
CTCTTTTTCC
GAAGGTAACT
GGCTTCAGCA
GAGCGCAGAT
ACCAAATACT
GTTCTTCTAG
TGTAGCCGTA
GTTAGGCCAC
CACTTCAAGA
ACTCTGTAGC
ACCGCCTACA
TACCTCGCTC
TGCTAATCCT
GTTACCAGTG
GCTGCTGCCA
GTGGCGATAA
GTCGTGTCTT
ACCGGGTTGG
ACTCAAGACG
ATAGTTACCG
GATAAGGCGC
AGCGGTCGGG
CTGAACGGGG
GGTTCGTGCA
CACAGCCCAG
CTTGGAGCGA
ACGACCTACA
CCGAACTGAG
ATACCTACAG
CGTGAGCTAT
GAGAAAGCGC
CACGCTTCCC
GAAGGGAGAA
AGGCGGACAG
GTATCCGGTA
AGCGGCAGGG
TCGGAACAGG
AGAGCGCACG
AGGGAGCTTC
CAGGGGGAAA
CGCCTGGTAT
CTTTATAGTC
CTGTCGGGTT
TCGCCACCTC
TGACTTGAGC
GTCGATTTTT
GTGATGCTCG
TCAGGGGGGC
GGAGCCTATG
GAAAAACGCC
AGCAACGCGG
CCTTTTTACG
GTTCCTGGCC
TTTTGCTGGC
CTTTTGCTCA
CATGTTCTTT
CCTGCGTTAT
CCCCTGATTC
TGTGGATAAC
CGTATTACCG
CCTTTGAGTG
AGCTGATACC
GCTCGCCGCA
GCCGAACGAC
CGAGCGCAGC
GAGTCAGTGA
GCGAGGAAGC
GGAAGAGCGC
CCAATACGCA
AACCGCCTCT
CCCCGCGCGT
TGGCCGATTC
ATTAATGCAG
CTGGCACGAC
AGGTTTCCCG
ACTGGAAAGC
GGGCAGTGAG
CGCAACGCAA
TTAATGTGAG
TTAGCTCACT
CATTAGGCAC
CCCAGGCTTT
ACACTTTATG
CTTCCGGCTC
GTATGTTGTG
TGGAATTGTG
AGCGGATAAC
AATTTCACAC
AGGAAACAGC
TATGACCATG
ATTACGCCAA
GCGCGCAATT
AACCCTCACT
AAAGGGAACA
AAAGCTGGAG
CTGCAAGCTT
AATGTAGTCT
TATGCAATAC
TCTTGTAGTC
TTGCAACATG
GTAACGATGA
GTTAGCAACA
TGCCTTACAA
GGAGAGAAAA
AGCACCGTGC
ATGCCGATTG
GTGGAAGTAA
GGTGGTACGA
TCGTGCCTTA
TTAGGAAGGC
AACAGACGGG
TCTGACATGG
ATTGGACGAA
CCACTGAATT
GCCGCATTGC
AGAGATATTG
TATTTAAGTG
CCTAGCTCGA
TACATAAACG
GGTCTCTCTG
GTTAGACCAG
ATCTGAGCCT
GGGAGCTCTC
TGGCTAACTA
GGGAACCCAC
TGCTTAAGCC
TCAATAAAGC
TTGCCTTGAG
TGCTTCAAGT
AGTGTGTGCC
CGTCTGTTGT
GTGACTCTGG
TAACTAGAGA
TCCCTCAGAC
CCTTTTAGTC
AGTGTGGAAA
ATCTCTAGCA
GTGGCGCCCG
AACAGGGACT
TGAAAGCGAA
AGGGAAACCA
GAGGAGCTCT
CTCGACGCAG
GACTCGGCTT
GCTGAAGCGC
GCACGGCAAG
AGGCGAGGGG
CGGCGACTGG
TGAGTACGCC
AAAAATTTTG
ACTAGCGGAG
GCTAGAAGGA
GAGAGATGGG
TGCGAGAGCG
TCAGTATTAA
GCGGGGGAGA
ATTAGATCGC
GATGGGAAAA
AATTCGGTTA
AGGCCAGGGG
GAAAGAAAAA
ATATAAATTA
AAACATATAG
TATGGGCAAG
CAGGGAGCTA
GAACGATTCG
CAGTTAATCC
TGGCCTGTTA
GAAACATCAG
AAGGCTGTAG
ACAAATACTG
GGACAGCTAC
AACCATCCCT
TCAGACAGGA
TCAGAAGAAC
TTAGATCATT
ATATAATACA
GTAGCAACCC
TCTATTGTGT
GCATCAAAGG
ATAGAGATAA
AAGACACCAA
GGAAGCTTTA
GACAAGATAG
AGGAAGAGCA
AAACAAAAGT
AAGACCACCG
CACAGCAAGC
GGCCGCTGAT
CTTCAGACCT
GGAGGAGGAG
ATATGAGGGA
CAATTGGAGA
AGTGAATTAT
ATAAATATAA
AGTAGTAAAA
ATTGAACCAT
TAGGAGTAGC
ACCCACCAAG
GCAAAGAGAA
GAGTGGTGCA
GAGAGAAAAA
AGAGCAGTGG
GAATAGGAGC
TTTGTTCCTT
GGGTTCTTGG
GAGCAGCAGG
AAGCACTATG
GGCGCAGCGT
CAATGACGCT
GACGGTACAG
GCCAGACAAT
TATTGTCTGG
TATAGTGCAG
CAGCAGAACA
ATTTGCTGAG
GGCTATTGAG
GCGCAACAGC
ATCTGTTGCA
ACTCACAGTC
TGGGGCATCA
AGCAGCTCCA
GGCAAGAATC
CTGGCTGTGG
AAAGATACCT
AAAGGATCAA
CAGCTCCTGG
GGATTTGGGG
TTGCTCTGGA
AAACTCATTT
GCACCACTGC
TGTGCCTTGG
AATGCTAGTT
GGAGTAATAA
ATCTCTGGAA
CAGATTTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGATG
TCGACGTTAA
CGCTAGCGAC
TGGGTCTGCG
ATGTCGACGT
TAACGCTAGC
GACTGGGTCT
GCGATAAGCT
TGGGAGTTCC
GCGTTACATA
ACTTACGGTA
AATGGCCCGC
CTGGCTGACC
GCCCAACGAC
CCCCGCCCAT
TGACGTCAAT
AATGACGTAT
GTTCCCATAG
TAACGCCAAT
AGGGACTTTC
CATTGACGTC
AATGGGTGGA
GTATTTACGG
TAAACTGCCC
ACTTGGCAGT
ACATCAAGTG
TATCATATGC
CAAGTACGCC
CCCTATTGAC
GTCAATGACG
GTAAATGGCC
CGCCTGGCAT
TATGCCCAGT
ACATGACCTT
ATGGGACTTT
CCTACTTGGC
AGTACATCTA
GTATTAGTCA
TCGCTATTAC
CATGGTGATG
CGGTTTTGGC
AGTACATCAA
TGGGCGTGGA
TAGCGGTTTG
ACTCACGGGG
ATTTCCAAGT
CTCCACCCCA
TTGACGTCAA
TGGGAGTTTG
TTTTGGCACC
AAAATCAACG
GGACTTTCCA
AAATGTCGTA
ACAACTCCGC
CCCATTGACG
CAAATGGGCG
GTAGGCGTGT
ACGGTGGGAG
GTCTATATAA
GCAGAGCTCT
CTGGCTAACT
AGAGAACCCA
CTGCTTACTG
GCTTATCGAA
ATTAATACGA
CTCACTATAG
GGAGACCCAA
GCTGGCTAGC
GTTTAAACTT
AAGCTTGGTA
CCGAGCTCGG
ATCCACTAGT
CCAGTGTGGT
GGAATTCTGC
AGATATCAAC
AAGTTTGTAC
AAAAAAGCTG
AACGAGAAAC
GTAAAATGAT
ATAAATATCA
ATATATTAAA
TTAGATTTTG
CATAAAAAAC
AGACTACATA
ATACTGTAAA
ACACAACATA
TCCAGTCACT
ATGGCGGCCG
CATTAGGCAC
CCCAGGCTTT
ACACTTTATG
CTTCCGGCTC
GTATAATGTG
TGGATTTTGA
GTTAGGATCC
GTCGAGATTT
TCAGGAGCTA
AGGAAGCTAA
AATGGAGAAA
AAAATCACTG
GATATACCAC
CGTTGATATA
TCCCAATGGC
ATCGTAAAGA
ACATTTTGAG
GCATTTCAGT
CAGTTGCTCA
ATGTACCTAT
AACCAGACCG
TTCAGCTGGA
TATTACGGCC
TTTTTAAAGA
CCGTAAAGAA
AAATAAGCAC
AAGTTTTATC
CGGCCTTTAT
TCACATTCTT
GCCCGCCTGA
TGAATGCTCA
TCCGGAATTC
CGTATGGCAA
TGAAAGACGG
TGAGCTGGTG
ATATGGGATA
GTGTTCACCC
TTGTTACACC
GTTTTCCATG
AGCAAACTGA
AACGTTTTCA
TCGCTCTGGA
GTGAATACCA
CGACGATTTC
CGGCAGTTTC
TACACATATA
TTCGCAAGAT
GTGGCGTGTT
ACGGTGAAAA
CCTGGCCTAT
TTCCCTAAAG
GGTTTATTGA
GAATATGTTT
TTCGTCTCAG
CCAATCCCTG
GGTGAGTTTC
ACCAGTTTTG
ATTTAAACGT
GGCCAATATG
GACAACTTCT
TCGCCCCCGT
TTTCACCATG
GGCAAATATT
ATACGCAAGG
CGACAAGGTG
CTGATGCCGC
TGGCGATTCA
GGTTCATCAT
GCCGTTTGTG
ATGGCTTCCA
TGTCGGCAGA
ATGCTTAATG
AATTACAACA
GTACTGCGAT
GAGTGGCAGG
GCGGGGCGTA
AAGATCTGGA
TCCGGCTTAC
TAAAAGCCAG
ATAACAGTAT
GCGTATTTGC
GCGCTGATTT
TTGCGGTATA
AGAATATATA
CTGATATGTA
TACCCGAAGT
ATGTCAAAAA
GAGGTATGCT
ATGAAGCAGC
GTATTACAGT
GACAGTTGAC
AGCGACAGCT
ATCAGTTGCT
CAAGGCATAT
ATGATGTCAA
TATCTCCGGT
CTGGTAAGCA
CAACCATGCA
GAATGAAGCC
CGTCGTCTGC
GTGCCGAACG
CTGGAAAGCG
GAAAATCAGG
AAGGGATGGC
TGAGGTCGCC
CGGTTTATTG
AAATGAACGG
CTCTTTTGCT
GACGAGAACA
GGGGCTGGTG
AAATGCAGTT
TAAGGTTTAC
ACCTATAAAA
GAGAGAGCCG
TTATCGTCTG
TTTGTGGATG
TACAGAGTGA
TATTATTGAC
ACGCCCGGGC
GACGGATGGT
GATCCCCCTG
GCCAGTGCAC
GTCTGCTGTC
AGATAAAGTC
TCCCGTGAAC
TTTACCCGGT
GGTGCATATC
GGGGATGAAA
GCTGGCGCAT
GATGACCACC
GATATGGCCA
GTGTGCCGGT
CTCCGTTATC
GGGGAAGAAG
TGGCTGATCT
CAGCCACCGC
GAAAATGACA
TCAAAAACGC
CATTAACCTG
ATGTTCTGGG
GAATATAAAT
GTCAGGCTCC
CTTATACACA
GCCAGTCTGC
AGGTCGACCA
TAGTGACTGG
ATATGTTGTG
TTTTACAGTA
TTATGTAGTC
TGTTTTTTAT
GCAAAATCTA
ATTTAATATA
TTGATATTTA
TATCATTTTA
CGTTTCTCGT
TCAGCTTTCT
TGTACAAAGT
GGTTGATTAC
AAAGACCATG
ACGGTGATTA
TAAAGATCAT
GACATCGACT
ACAAGGATGA
CGATGACAAG
GGCTCGGGCT
CGACCTCGGG
TAAGCCTATC
CCTAACCCTC
TCCTCGGTCT
CGATTCTACA
TGATAACTCG
AGTCTAGAGG
GCCCGCGGTT
CGAAGGTAAG
CCTATCCCTA
ACCCTCTCCT
CGGTCTCGAT
TCTACGCGTA
CCGGTTAGTA
ATGACGATGG
TACCTACCGG
GTAGGGGAGG
CGCTTTTCCC
AAGGCAGTCT
GGAGCATGCG
CTTTAGCAGC
CCCGCTGGGC
ACTTGGCGCT
ACACAAGTGG
CCTCTGGCCT
CGCACACATT
CCACATCCAC
CGGTAGGCGC
CAACCGGCTC
CGTTCTTTGG
TGGCCCCTTC
GCGCCACCTT
CTACTCCTCC
CCTAGTCAGG
AAGTTCCCCC
CC