名称: | pLenti-U6-MCS-sgRNA-SV40-MS2-P65-HSF1-Neo |
---|---|
别称: | |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | 空载体 |
原核抗性: | Amp |
筛选标记: | Neo/G418 |
荧光标记: | |
启动子: | U6 |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | U6 |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GTCGACGGAT
CGGGAGATCT
CCCGATCCCC
TATGGTGCAC
TCTCAGTACA
ATCTGCTCTG
ATGCCGCATA
GTTAAGCCAG
TATCTGCTCC
CTGCTTGTGT
GTTGGAGGTC
GCTGAGTAGT
GCGCGAGCAA
AATTTAAGCT
ACAACAAGGC
AAGGCTTGAC
CGACAATTGC
ATGAAGAATC
TGCTTAGGGT
TAGGCGTTTT
GCGCTGCTTC
GCGATGTACG
GGCCAGATAT
ACGCGTTGAC
ATTGATTATT
GACTAGTTAT
TAATAGTAAT
CAATTACGGG
GTCATTAGTT
CATAGCCCAT
ATATGGAGTT
CCGCGTTACA
TAACTTACGG
TAAATGGCCC
GCCTGGCTGA
CCGCCCAACG
ACCCCCGCCC
ATTGACGTCA
ATAATGACGT
ATGTTCCCAT
AGTAACGCCA
ATAGGGACTT
TCCATTGACG
TCAATGGGTG
GAGTATTTAC
GGTAAACTGC
CCACTTGGCA
GTACATCAAG
TGTATCATAT
GCCAAGTACG
CCCCCTATTG
ACGTCAATGA
CGGTAAATGG
CCCGCCTGGC
ATTATGCCCA
GTACATGACC
TTATGGGACT
TTCCTACTTG
GCAGTACATC
TACGTATTAG
TCATCGCTAT
TACCATGGTG
ATGCGGTTTT
GGCAGTACAT
CAATGGGCGT
GGATAGCGGT
TTGACTCACG
GGGATTTCCA
AGTCTCCACC
CCATTGACGT
CAATGGGAGT
TTGTTTTGGC
ACCAAAATCA
ACGGGACTTT
CCAAAATGTC
GTAACAACTC
CGCCCCATTG
ACGCAAATGG
GCGGTAGGCG
TGTACGGTGG
GAGGTCTATA
TAAGCAGCGC
GTTTTGCCTG
TACTGGGTCT
CTCTGGTTAG
ACCAGATCTG
AGCCTGGGAG
CTCTCTGGCT
AACTAGGGAA
CCCACTGCTT
AAGCCTCAAT
AAAGCTTGCC
TTGAGTGCTT
CAAGTAGTGT
GTGCCCGTCT
GTTGTGTGAC
TCTGGTAACT
AGAGATCCCT
CAGACCCTTT
TAGTCAGTGT
GGAAAATCTC
TAGCAGTGGC
GCCCGAACAG
GGACTTGAAA
GCGAAAGGGA
AACCAGAGGA
GCTCTCTCGA
CGCAGGACTC
GGCTTGCTGA
AGCGCGCACG
GCAAGAGGCG
AGGGGCGGCG
ACTGGTGAGT
ACGCCAAAAA
TTTTGACTAG
CGGAGGCTAG
AAGGAGAGAG
ATGGGTGCGA
GAGCGTCAGT
ATTAAGCGGG
GGAGAATTAG
ATCGCGATGG
GAAAAAATTC
GGTTAAGGCC
AGGGGGAAAG
AAAAAATATA
AATTAAAACA
TATAGTATGG
GCAAGCAGGG
AGCTAGAACG
ATTCGCAGTT
AATCCTGGCC
TGTTAGAAAC
ATCAGAAGGC
TGTAGACAAA
TACTGGGACA
GCTACAACCA
TCCCTTCAGA
CAGGATCAGA
AGAACTTAGA
TCATTATATA
ATACAGTAGC
AACCCTCTAT
TGTGTGCATC
AAAGGATAGA
GATAAAAGAC
ACCAAGGAAG
CTTTAGACAA
GATAGAGGAA
GAGCAAAACA
AAAGTAAGAC
CACCGCACAG
CAAGCGGCCG
CTGATCTTCA
GACCTGGAGG
AGGAGATATG
AGGGACAATT
GGAGAAGTGA
ATTATATAAA
TATAAAGTAG
TAAAAATTGA
ACCATTAGGA
GTAGCACCCA
CCAAGGCAAA
GAGAAGAGTG
GTGCAGAGAG
AAAAAAGAGC
AGTGGGAATA
GGAGCTTTGT
TCCTTGGGTT
CTTGGGAGCA
GCAGGAAGCA
CTATGGGCGC
AGCGTCAATG
ACGCTGACGG
TACAGGCCAG
ACAATTATTG
TCTGGTATAG
TGCAGCAGCA
GAACAATTTG
CTGAGGGCTA
TTGAGGCGCA
ACAGCATCTG
TTGCAACTCA
CAGTCTGGGG
CATCAAGCAG
CTCCAGGCAA
GAATCCTGGC
TGTGGAAAGA
TACCTAAAGG
ATCAACAGCT
CCTGGGGATT
TGGGGTTGCT
CTGGAAAACT
CATTTGCACC
ACTGCTGTGC
CTTGGAATGC
TAGTTGGAGT
AATAAATCTC
TGGAACAGAT
TTGGAATCAC
ACGACCTGGA
TGGAGTGGGA
CAGAGAAATT
AACAATTACA
CAAGCTTAAT
ACACTCCTTA
ATTGAAGAAT
CGCAAAACCA
GCAAGAAAAG
AATGAACAAG
AATTATTGGA
ATTAGATAAA
TGGGCAAGTT
TGTGGAATTG
GTTTAACATA
ACAAATTGGC
TGTGGTATAT
AAAATTATTC
ATAATGATAG
TAGGAGGCTT
GGTAGGTTTA
AGAATAGTTT
TTGCTGTACT
TTCTATAGTG
AATAGAGTTA
GGCAGGGATA
TTCACCATTA
TCGTTTCAGA
CCCACCTCCC
AACCCCGAGG
GGACCCGACA
GGCCCGAAGG
AATAGAAGAA
GAAGGTGGAG
AGAGAGACAG
AGACAGATCC
ATTCGATTAG
TGAACGGATC
GGCACTGCGT
GCGCCAATTC
TGCAGACAAA
TGGCAGTATT
CATCCACAAT
TTTAAAAGAA
AAGGGGGGAT
TGGGGGGTAC
AGTGCAGGGG
AAAGAATAGT
AGACATAATA
GCAACAGACA
TACAAACTAA
AGAATTACAA
AAACAAATTA
CAAAAATTCA
AAATTTTCGG
GTTTATTACA
GGGACAGCAG
AGATCCAGTT
TGGTTAATTA
GCTAGCGAGG
GCCTATTTCC
CATGATTCCT
TCATATTTGC
ATATACGATA
CAAGGCTGTT
AGAGAGATAA
TTAGAATTAA
TTTGACTGTA
AACACAAAGA
TATTAGTACA
AAATACGTGA
CGTAGAAAGT
AATAATTTCT
TGGGTAGTTT
GCAGTTTTAA
AATTATGTTT
TAAAATGGAC
TATCATATGC
TTACCGTAAC
TTGAAAGTAT
TTCGATTTCT
TGGCTTTATA
TATCTTGTGG
AAAGGACGAA
ACACCGGAGA
CGGACGTCTC
TGTTTTAGAG
CTAGAAATAG
CAAGTTAAAA
TAAGGCTAGT
CCGTTATCAA
CTTGAAAAAG
TGGCACCGAG
TCGGTGCTTT
TTTGAATTCG
CTAGCTAGGT
CTTGAAAGGG
TGTGTCAGTT
AGGGTGTGGA
AAGTCCCCAG
GCTCCCCAGC
AGGCAGAAGT
ATGCAAAGCA
TGCATCTCAA
TTAGTCAGCA
ACCAGGTGTG
GAAAGTCCCC
AGGCTCCCCA
GCAGGCAGAA
GTATGCAAAG
CATGCATCTC
AATTAGTCAG
CAACCATAGT
CCCGCCCCTA
ACTCCGCCCA
TCCCGCCCCT
AACTCCGCCC
AGTTCCGCCC
ATTCTCCGCC
CCATGGCTGA
CTAATTTTTT
TTATTTATGC
AGAGGCCGAG
GCCGCCTCTG
CCTCTGAGCT
ATTCCAGAAG
TAGTGAGGAG
GCTTTTTTGG
AGGCCTAGGC
TTTTGCAAAC
GTACGGCCAC
CATGGCTTCA
AACTTTACTC
AGTTCGTGCT
CGTGGACAAT
GGTGGGACAG
GGGATGTGAC
AGTGGCTCCT
TCTAATTTCG
CTAATGGGGT
GGCAGAGTGG
ATCAGCTCCA
ACTCACGGAG
CCAGGCCTAC
AAGGTGACAT
GCAGCGTCAG
GCAGTCTAGT
GCCCAGAAGA
GAAAGTATAC
CATCAAGGTG
GAGGTCCCCA
AAGTGGCTAC
CCAGACAGTG
GGCGGAGTCG
AACTGCCTGT
CGCCGCTTGG
AGGTCCTACC
TGAACATGGA
GCTCACTATC
CCAATTTTCG
CTACCAATTC
TGACTGTGAA
CTCATCGTGA
AGGCAATGCA
GGGGCTCCTC
AAAGACGGTA
ATCCTATCCC
TTCCGCCATC
GCCGCTAACT
CAGGTATCTA
CAGCGCTGGA
GGAGGTGGAA
GCGGAGGAGG
AGGAAGCGGA
GGAGGAGGTA
GCGGACCTAA
GAAAAAGAGG
AAGGTGGCGG
CCGCTGGATC
CCCTTCAGGG
CAGATCAGCA
ACCAGGCCCT
GGCTCTGGCC
CCTAGCTCCG
CTCCAGTGCT
GGCCCAGACT
ATGGTGCCCT
CTAGTGCTAT
GGTGCCTCTG
GCCCAGCCAC
CTGCTCCAGC
CCCTGTGCTG
ACCCCAGGAC
CACCCCAGTC
ACTGAGCGCT
CCAGTGCCCA
AGTCTACACA
GGCCGGCGAG
GGGACTCTGA
GTGAAGCTCT
GCTGCACCTG
CAGTTCGACG
CTGATGAGGA
CCTGGGAGCT
CTGCTGGGGA
ACAGCACCGA
TCCCGGAGTG
TTCACAGATC
TGGCCTCCGT
GGACAACTCT
GAGTTTCAGC
AGCTGCTGAA
TCAGGGCGTG
TCCATGTCTC
ATAGTACAGC
CGAACCAATG
CTGATGGAGT
ACCCCGAAGC
CATTACCCGG
CTGGTGACCG
GCAGCCAGCG
GCCCCCCGAC
CCCGCTCCAA
CTCCCCTGGG
AACCAGCGGC
CTGCCTAATG
GGCTGTCCGG
AGATGAAGAC
TTCTCAAGCA
TCGCTGATAT
GGACTTTAGT
GCCCTGCTGT
CACAGATTTC
CTCTAGTGGG
CAGGGAGGAG
GTGGAAGCGG
CTTCAGCGTG
GACACCAGTG
CCCTGCTGGA
CCTGTTCAGC
CCCTCGGTGA
CCGTGCCCGA
CATGAGCCTG
CCTGACCTTG
ACAGCAGCCT
GGCCAGTATC
CAAGAGCTCC
TGTCTCCCCA
GGAGCCCCCC
AGGCCTCCCG
AGGCAGAGAA
CAGCAGCCCG
GATTCAGGGA
AGCAGCTGGT
GCACTACACA
GCGCAGCCGC
TGTTCCTGCT
GGACCCCGGC
TCCGTGGACA
CCGGGAGCAA
CGACCTGCCG
GTGCTGTTTG
AGCTGGGAGA
GGGCTCCTAC
TTCTCCGAAG
GGGACGGCTT
CGCCGAGGAC
CCCACCATCT
CCCTGCTGAC
AGGCTCGGAG
CCTCCCAAAG
CCAAGGACCC
CACTGTCTCC
TGTACAGGAT
CTCGCGAGGG
CAGAGGAAGT
CTTCTAACAT
GCGGTGACGT
GGAGGAGAAT
CCCGGCCCTG
CCACCATGGC
TGAACAAGAT
GGATTGCACG
CAGGTTCTCC
GGCCGCTTGG
GTGGAGAGGC
TATTCGGCTA
TGACTGGGCA
CAACAGACAA
TCGGCTGCTC
TGATGCCGCC
GTGTTCCGGC
TGTCAGCGCA
GGGGCGCCCG
GTTCTTTTTG
TCAAGACCGA
CCTGTCCGGT
GCCCTGAATG
AACTGCAGGA
CGAGGCAGCG
CGGCTATCGT
GGCTGGCCAC
GACGGGCGTT
CCTTGCGCAG
CTGTGCTCGA
CGTTGTCACT
GAAGCGGGAA
GGGACTGGCT
GCTATTGGGC
GAAGTGCCGG
GGCAGGATCT
CCTGTCATCT
CACCTTGCTC
CTGCCGAGAA
AGTATCCATC
ATGGCTGATG
CAATGCGGCG
GCTGCATACG
CTTGATCCGG
CTACCTGCCC
ATTCGACCAC
CAAGCGAAAC
ATCGCATCGA
GCGAGCACGT
ACTCGGATGG
AAGCCGGTCT
TGTCGATCAG
GATGATCTGG
ACGAAGAGCA
TCAGGGGCTC
GCGCCAGCCG
AACTGTTCGC
CAGGCTCAAG
GCGCGCATGC
CCGACGGCGA
GGATCTCGTC
GTGACCCATG
GCGATGCCTG
CTTGCCGAAT
ATCATGGTGG
AAAATGGCCG
CTTTTCTGGA
TTCATCGACT
GTGGCCGGCT
GGGTGTGGCG
GACCGCTATC
AGGACATAGC
GTTGGCTACC
CGTGATATTG
CTGAAGAGCT
TGGCGGCGAA
TGGGCTGACC
GCTTCCTCGT
GCTTTACGGT
ATCGCCGCTC
CCGATTCGCA
GCGCATCGCC
TTCTATCGCC
TTCTTGACGA
GTTCTTCTGA
GAATTCGATA
TCAAGCTTAT
CGGTAATCAA
CCTCTGGATT
ACAAAATTTG
TGAAAGATTG
ACTGGTATTC
TTAACTATGT
TGCTCCTTTT
ACGCTATGTG
GATACGCTGC
TTTAATGCCT
TTGTATCATG
CTATTGCTTC
CCGTATGGCT
TTCATTTTCT
CCTCCTTGTA
TAAATCCTGG
TTGCTGTCTC
TTTATGAGGA
GTTGTGGCCC
GTTGTCAGGC
AACGTGGCGT
GGTGTGCACT
GTGTTTGCTG
ACGCAACCCC
CACTGGTTGG
GGCATTGCCA
CCACCTGTCA
GCTCCTTTCC
GGGACTTTCG
CTTTCCCCCT
CCCTATTGCC
ACGGCGGAAC
TCATCGCCGC
CTGCCTTGCC
CGCTGCTGGA
CAGGGGCTCG
GCTGTTGGGC
ACTGACAATT
CCGTGGTGTT
GTCGGGGAAA
TCATCGTCCT
TTCCTTGGCT
GCTCGCCTGT
GTTGCCACCT
GGATTCTGCG
CGGGACGTCC
TTCTGCTACG
TCCCTTCGGC
CCTCAATCCA
GCGGACCTTC
CTTCCCGCGG
CCTGCTGCCG
GCTCTGCGGC
CTCTTCCGCG
TCTTCGCCTT
CGCCCTCAGA
CGAGTCGGAT
CTCCCTTTGG
GCCGCCTCCC
CGCATCGATA
CCGTCGACCT
CGAGACCTAG
AAAAACATGG
AGCAATCACA
AGTAGCAATA
CAGCAGCTAC
CAATGCTGAT
TGTGCCTGGC
TAGAAGCACA
AGAGGAGGAG
GAGGTGGGTT
TTCCAGTCAC
ACCTCAGGTA
CCTTTAAGAC
CAATGACTTA
CAAGGCAGCT
GTAGATCTTA
GCCACTTTTT
AAAAGAAAAG
GGGGGACTGG
AAGGGCTAAT
TCACTCCCAA
CGAAGACAAG
ATATCCTTGA
TCTGTGGATC
TACCACACAC
AAGGCTACTT
CCCTGATTGG
CAGAACTACA
CACCAGGGCC
AGGGATCAGA
TATCCACTGA
CCTTTGGATG
GTGCTACAAG
CTAGTACCAG
TTGAGCAAGA
GAAGGTAGAA
GAAGCCAATG
AAGGAGAGAA
CACCCGCTTG
TTACACCCTG
TGAGCCTGCA
TGGGATGGAT
GACCCGGAGA
GAGAAGTATT
AGAGTGGAGG
TTTGACAGCC
GCCTAGCATT
TCATCACATG
GCCCGAGAGC
TGCATCCGGA
CTGTACTGGG
TCTCTCTGGT
TAGACCAGAT
CTGAGCCTGG
GAGCTCTCTG
GCTAACTAGG
GAACCCACTG
CTTAAGCCTC
AATAAAGCTT
GCCTTGAGTG
CTTCAAGTAG
TGTGTGCCCG
TCTGTTGTGT
GACTCTGGTA
ACTAGAGATC
CCTCAGACCC
TTTTAGTCAG
TGTGGAAAAT
CTCTAGCAGG
GCCCGTTTAA
ACCCGCTGAT
CAGCCTCGAC
TGTGCCTTCT
AGTTGCCAGC
CATCTGTTGT
TTGCCCCTCC
CCCGTGCCTT
CCTTGACCCT
GGAAGGTGCC
ACTCCCACTG
TCCTTTCCTA
ATAAAATGAG
GAAATTGCAT
CGCATTGTCT
GAGTAGGTGT
CATTCTATTC
TGGGGGGTGG
GGTGGGGCAG
GACAGCAAGG
GGGAGGATTG
GGAAGACAAT
AGCAGGCATG
CTGGGGATGC
GGTGGGCTCT
ATGGCTTCTG
AGGCGGAAAG
AACCAGCTGG
GGCTCTAGGG
GGTATCCCCA
CGCGCCCTGT
AGCGGCGCAT
TAAGCGCGGC
GGGTGTGGTG
GTTACGCGCA
GCGTGACCGC
TACACTTGCC
AGCGCCCTAG
CGCCCGCTCC
TTTCGCTTTC
TTCCCTTCCT
TTCTCGCCAC
GTTCGCCGGC
TTTCCCCGTC
AAGCTCTAAA
TCGGGGGCTC
CCTTTAGGGT
TCCGATTTAG
TGCTTTACGG
CACCTCGACC
CCAAAAAACT
TGATTAGGGT
GATGGTTCAC
GTAGTGGGCC
ATCGCCCTGA
TAGACGGTTT
TTCGCCCTTT
GACGTTGGAG
TCCACGTTCT
TTAATAGTGG
ACTCTTGTTC
CAAACTGGAA
CAACACTCAA
CCCTATCTCG
GTCTATTCTT
TTGATTTATA
AGGGATTTTG
CCGATTTCGG
CCTATTGGTT
AAAAAATGAG
CTGATTTAAC
AAAAATTTAA
CGCGAATTAA
TTCTGTGGAA
TGTGTGTCAG
TTAGGGTGTG
GAAAGTCCCC
AGGCTCCCCA
GCAGGCAGAA
GTATGCAAAG
CATGCATCTC
AATTAGTCAG
CAACCAGGTG
TGGAAAGTCC
CCAGGCTCCC
CAGCAGGCAG
AAGTATGCAA
AGCATGCATC
TCAATTAGTC
AGCAACCATA
GTCCCGCCCC
TAACTCCGCC
CATCCCGCCC
CTAACTCCGC
CCAGTTCCGC
CCATTCTCCG
CCCCATGGCT
GACTAATTTT
TTTTATTTAT
GCAGAGGCCG
AGGCCGCCTC
TGCCTCTGAG
CTATTCCAGA
AGTAGTGAGG
AGGCTTTTTT
GGAGGCCTAG
GCTTTTGCAA
AAAGCTCCCG
GGAGCTTGTA
TATCCATTTT
CGGATCTGAT
CAGCACGTGT
TGACAATTAA
TCATCGGCAT
AGTATATCGG
CATAGTATAA
TACGACAAGG
TGAGGAACTA
AACCATGGCC
AAGTTGACCA
GTGCCGTTCC
GGTGCTCACC
GCGCGCGACG
TCGCCGGAGC
GGTCGAGTTC
TGGACCGACC
GGCTCGGGTT
CTCCCGGGAC
TTCGTGGAGG
ACGACTTCGC
CGGTGTGGTC
CGGGACGACG
TGACCCTGTT
CATCAGCGCG
GTCCAGGACC
AGGTGGTGCC
GGACAACACC
CTGGCCTGGG
TGTGGGTGCG
CGGCCTGGAC
GAGCTGTACG
CCGAGTGGTC
GGAGGTCGTG
TCCACGAACT
TCCGGGACGC
CTCCGGGCCG
GCCATGACCG
AGATCGGCGA
GCAGCCGTGG
GGGCGGGAGT
TCGCCCTGCG
CGACCCGGCC
GGCAACTGCG
TGCACTTCGT
GGCCGAGGAG
CAGGACTGAC
ACGTGCTACG
AGATTTCGAT
TCCACCGCCG
CCTTCTATGA
AAGGTTGGGC
TTCGGAATCG
TTTTCCGGGA
CGCCGGCTGG
ATGATCCTCC
AGCGCGGGGA
TCTCATGCTG
GAGTTCTTCG
CCCACCCCAA
CTTGTTTATT
GCAGCTTATA
ATGGTTACAA
ATAAAGCAAT
AGCATCACAA
ATTTCACAAA
TAAAGCATTT
TTTTCACTGC
ATTCTAGTTG
TGGTTTGTCC
AAACTCATCA
ATGTATCTTA
TCATGTCTGT
ATACCGTCGA
CCTCTAGCTA
GAGCTTGGCG
TAATCATGGT
CATAGCTGTT
TCCTGTGTGA
AATTGTTATC
CGCTCACAAT
TCCACACAAC
ATACGAGCCG
GAAGCATAAA
GTGTAAAGCC
TGGGGTGCCT
AATGAGTGAG
CTAACTCACA
TTAATTGCGT
TGCGCTCACT
GCCCGCTTTC
CAGTCGGGAA
ACCTGTCGTG
CCAGCTGCAT
TAATGAATCG
GCCAACGCGC
GGGGAGAGGC
GGTTTGCGTA
TTGGGCGCTC
TTCCGCTTCC
TCGCTCACTG
ACTCGCTGCG
CTCGGTCGTT
CGGCTGCGGC
GAGCGGTATC
AGCTCACTCA
AAGGCGGTAA
TACGGTTATC
CACAGAATCA
GGGGATAACG
CAGGAAAGAA
CATGTGAGCA
AAAGGCCAGC
AAAAGGCCAG
GAACCGTAAA
AAGGCCGCGT
TGCTGGCGTT
TTTCCATAGG
CTCCGCCCCC
CTGACGAGCA
TCACAAAAAT
CGACGCTCAA
GTCAGAGGTG
GCGAAACCCG
ACAGGACTAT
AAAGATACCA
GGCGTTTCCC
CCTGGAAGCT
CCCTCGTGCG
CTCTCCTGTT
CCGACCCTGC
CGCTTACCGG
ATACCTGTCC
GCCTTTCTCC
CTTCGGGAAG
CGTGGCGCTT
TCTCATAGCT
CACGCTGTAG
GTATCTCAGT
TCGGTGTAGG
TCGTTCGCTC
CAAGCTGGGC
TGTGTGCACG
AACCCCCCGT
TCAGCCCGAC
CGCTGCGCCT
TATCCGGTAA
CTATCGTCTT
GAGTCCAACC
CGGTAAGACA
CGACTTATCG
CCACTGGCAG
CAGCCACTGG
TAACAGGATT
AGCAGAGCGA
GGTATGTAGG
CGGTGCTACA
GAGTTCTTGA
AGTGGTGGCC
TAACTACGGC
TACACTAGAA
GAACAGTATT
TGGTATCTGC
GCTCTGCTGA
AGCCAGTTAC
CTTCGGAAAA
AGAGTTGGTA
GCTCTTGATC
CGGCAAACAA
ACCACCGCTG
GTAGCGGTGG
TTTTTTTGTT
TGCAAGCAGC
AGATTACGCG
CAGAAAAAAA
GGATCTCAAG
AAGATCCTTT
GATCTTTTCT
ACGGGGTCTG
ACGCTCAGTG
GAACGAAAAC
TCACGTTAAG
GGATTTTGGT
CATGAGATTA
TCAAAAAGGA
TCTTCACCTA
GATCCTTTTA
AATTAAAAAT
GAAGTTTTAA
ATCAATCTAA
AGTATATATG
AGTAAACTTG
GTCTGACAGT
TACCAATGCT
TAATCAGTGA
GGCACCTATC
TCAGCGATCT
GTCTATTTCG
TTCATCCATA
GTTGCCTGAC
TCCCCGTCGT
GTAGATAACT
ACGATACGGG
AGGGCTTACC
ATCTGGCCCC
AGTGCTGCAA
TGATACCGCG
AGACCCACGC
TCACCGGCTC
CAGATTTATC
AGCAATAAAC
CAGCCAGCCG
GAAGGGCCGA
GCGCAGAAGT
GGTCCTGCAA
CTTTATCCGC
CTCCATCCAG
TCTATTAATT
GTTGCCGGGA
AGCTAGAGTA
AGTAGTTCGC
CAGTTAATAG
TTTGCGCAAC
GTTGTTGCCA
TTGCTACAGG
CATCGTGGTG
TCACGCTCGT
CGTTTGGTAT
GGCTTCATTC
AGCTCCGGTT
CCCAACGATC
AAGGCGAGTT
ACATGATCCC
CCATGTTGTG
CAAAAAAGCG
GTTAGCTCCT
TCGGTCCTCC
GATCGTTGTC
AGAAGTAAGT
TGGCCGCAGT
GTTATCACTC
ATGGTTATGG
CAGCACTGCA
TAATTCTCTT
ACTGTCATGC
CATCCGTAAG
ATGCTTTTCT
GTGACTGGTG
AGTACTCAAC
CAAGTCATTC
TGAGAATAGT
GTATGCGGCG
ACCGAGTTGC
TCTTGCCCGG
CGTCAATACG
GGATAATACC
GCGCCACATA
GCAGAACTTT
AAAAGTGCTC
ATCATTGGAA
AACGTTCTTC
GGGGCGAAAA
CTCTCAAGGA
TCTTACCGCT
GTTGAGATCC
AGTTCGATGT
AACCCACTCG
TGCACCCAAC
TGATCTTCAG
CATCTTTTAC
TTTCACCAGC
GTTTCTGGGT
GAGCAAAAAC
AGGAAGGCAA
AATGCCGCAA
AAAAGGGAAT
AAGGGCGACA
CGGAAATGTT
GAATACTCAT
ACTCTTCCTT
TTTCAATATT
ATTGAAGCAT
TTATCAGGGT
TATTGTCTCA
TGAGCGGATA
CATATTTGAA
TGTATTTAGA
AAAATAAACA
AATAGGGGTT
CCGCGCACAT
TTCCCCGAAA
AGTGCCACCT
GAC