名称: | p0521s |
---|---|
别称: | Plasmid#62862,提取要加诱导剂 |
基因长: | |
质粒宿主: | 大肠杆菌 |
质粒用途: | 人工染色体 |
片段类型: | cDNA |
片段物种: | 空载体 |
原核抗性: | Chl |
筛选标记: | |
荧光标记: | |
启动子: | |
复制子: | Ori2 |
拷贝数: | 极低 |
感受态: | EPI300 |
温度: | 30度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
TCGAGCTGGT
TGCCCTCGCC
GCTGGGCTGG
CGGCCGTCTA
TGGCCCTGCA
AACGCGCCAG
AAACGCCGTC
GAAGCCGTGT
GCGAGACACC
GCGGCCGGCC
GCCGGCGTTG
TGGATACCTC
GCGGAAAACT
TGGCCCTCAC
TGACAGATGA
GGGGCGGACG
TTGACACTTG
AGGGGCCGAC
TCACCCGGCG
CGGCGTTGAC
AGATGAGGGG
CAGGCTCGAT
TTCGGCCGGC
GACGTGGAGC
TGGCCAGCCT
CGCAAATCGG
CGAAAACGCC
TGATTTTACG
CGAGTTTCCC
ACAGATGATG
TGGACAAGCC
TGGGGATAAG
TGCCCTGCGG
TATTGACACT
TGAGGGGCGC
GACTACTGAC
AGATGAGGGG
CGCGATCCTT
GACACTTGAG
GGGCAGAGTG
CTGACAGATG
AGGGGCGCAC
CTATTGACAT
TTGAGGGGCT
GTCCACAGGC
AGAAAATCCA
GCATTTGCAA
GGGTTTCCGC
CCGTTTTTCG
GCCACCGCTA
ACCTGTCTTT
TAACCTGCTT
TTAAACCAAT
ATTTATAAAC
CTTGTTTTTA
ACCAGGGCTG
CGCCCTGTGC
GCGTGACCGC
GCACGCCGAA
GGGGGGTGCC
CCCCCTTCTC
GAACCCTCCC
GGTCGAGTGA
GCGAGGAAGC
ACCAGGGAAC
AGCACTTATA
TATTCTGCTT
ACACACGATG
CCTGAAAAAA
CTTCCCTTGG
GGTTATCCAC
TTATCCACGG
GGATATTTTT
ATAATTATTT
TTTTTATAGT
TTTTAGATCT
TCTTTTTTAG
AGCGCCTTGT
AGGCCTTTAT
CCATGCTGGT
TCTAGAGAAG
GTGTTGTGAC
AAATTGCCCT
TTCAGTGTGA
CAAATCACCC
TCAAATGACA
GTCCTGTCTG
TGACAAATTG
CCCTTAACCC
TGTGACAAAT
TGCCCTCAGA
AGAAGCTGTT
TTTTCACAAA
GTTATCCCTG
CTTATTGACT
CTTTTTTATT
TAGTGTGACA
ATCTAAAAAC
TTGTCACACT
TCACATGGAT
CTGTCATGGC
GGAAACAGCG
GTTATCAATC
ACAAGAAACG
TAAAAATAGC
CCGCGAATCG
TCCAGTCAAA
CGACCTCACT
GAGGCGGCAT
ATAGTCTCTC
CCGGGATCAA
AAACGTATGC
TGTATCTGTT
CGTTGACCAG
ATCAGAAAAT
CTGATGGCAC
CCTACAGGAA
CATGACGGTA
TCTGCGAGAT
CCATGTTGCT
AAATATGCTG
AAATATTCGG
ATTGACCTCT
GCGGAAGCCA
GTAAGGATAT
ACGGCAGGCA
TTGAAGAGTT
TCGCGGGGAA
GGAAGTGGTT
TTTTATCGCC
CTGAAGAGGA
TGCCGGCGAT
GAAAAAGGCT
ATGAATCTTT
TCCTTGGTTT
ATCAAACGTG
CGCACAGTCC
ATCCAGAGGG
CTTTACAGTG
TACATATCAA
CCCATATCTC
ATTCCCTTCT
TTATCGGGTT
ACAGAACCGG
TTTACGCAGT
TTCGGCTTAG
TGAAACAAAA
GAAATCACCA
ATCCGTATGC
CATGCGTTTA
TACGAATCCC
TGTGTCAGTA
TCGTAAGCCG
GATGGCTCAG
GCATCGTCTC
TCTGAAAATC
GACTGGATCA
TAGAGCGTTA
CCAGCTGCCT
CAAAGTTACC
AGCGTATGCC
TGACTTCCGC
CGCCGCTTCC
TGCAGGTCTG
TGTTAATGAG
ATCAACAGCA
GAACTCCAAT
GCGCCTCTCA
TACATTGAGA
AAAAGAAAGG
CCGCCAGACG
ACTCATATCG
TATTTTCCTT
CCGCGATATC
ACTTCCATGA
CGACAGGATA
GTCTGAGGGT
TATCTGTCAC
AGATTTGAGG
GTGGTTCGTC
ACATTTGTTC
TGACCTACTG
AGGGTAATTT
GTCACAGTTT
TGCTGTTTCC
TTCAGCCTGC
ATGGATTTTC
TCATACTTTT
TGAACTGTAA
TTTTTAAGGA
AGCCAAATTT
GAGGGCAGTT
TGTCACAGTT
GATTTCCTTC
TCTTTCCCTT
CGTCATGTGA
CCTGATATCG
GGGGTTAGTT
CGTCATCATT
GATGAGGGTT
GATTATCACA
GTTTATTACT
CTGAATTGGC
TATCCGCGTG
TGTACCTCTA
CCTGGAGTTT
TTCCCACGGT
GGATATTTCT
TCTTGCGCTG
AGCGTAAGAG
CTATCTGACA
GAACAGTTCT
TCTTTGCTTC
CTCGCCAGTT
CGCTCGCTAT
GCTCGGTTAC
ACGGCTGCGG
CGAGCATCAC
GTGCTATAAA
AATAATTATA
ATTTAAATTT
TTTAATATAA
ATATATAAAT
TAAAAATAGA
AAGTAAAAAA
AGAAATTAAA
GAAAAAATAG
TTTTTGTTTT
CCGAAGATGT
AAAAGACTCT
AGGGGGATCG
CCAACAAATA
CTACCTTTTA
CCTTGCTCTT
CCTGCTCTCA
GGTATTAATG
CCGAATTGTT
TCATCTTGTC
TGTGTAGAAG
ACCACACACG
AAAATCCTGT
GATTTTACAT
TTTACTTATC
GTTAATCGAA
TGTATATCTA
TTTAATCTGC
TTTTCTTGTC
TAATAAATAT
ATATGTAAAG
TACGCTTTTT
GTTGAAATTT
TTTAAACCTT
TGTTTATTTT
TTTTTCTTCA
TTCCGTAACT
CTTCTACCTT
CTTTATTTAC
TTTCTAAAAT
CCAAATACAA
AACATAAAAA
TAAATAAACA
CAGAGTAAAT
TCCCAAATTA
TTCCATCATT
AAAAGATACG
AGGCGCGTGT
AAGTTACAGG
CAAGCGATCC
TAGTACACTC
TATATTTTTT
TATGCCTCGG
TAATGATTTT
CATTTTTTTT
TTCCACCTAG
CGGATGACTC
TTTTTTTTTC
TTAGCGATTG
GCATTATCAC
ATAATGAATT
ATACATTATA
TAAAGTAATG
TGATTTCTTC
GAAGAATATA
CTAAAAAATG
AGCAGGCAAG
ATAAACGAAG
GCAAAGATGA
CAGAGCAGAA
AGCCCTAGTA
AAGCGTATTA
CAAATGAAAC
CAAGATTCAG
ATTGCGATCT
CTTTAAAGGG
TGGTCCCCTA
GCGATAGAGC
ACTCGATCTT
CCCAGAAAAA
GAGGCAGAAG
CAGTAGCAGA
ACAGGCCACA
CAATCGCAAG
TGATTAACGT
CCACACAGGT
ATAGGGTTTC
TGGACCATAT
GATACATGCT
CTGGCCAAGC
ATTCCGGCTG
GTCGCTAATC
GTTGAGTGCA
TTGGTGACTT
ACACATAGAC
GACCATCACA
CCACTGAAGA
CTGCGGGATT
GCTCTCGGTC
AAGCTTTTAA
AGAGGCCCTA
GGGGCCGTGC
GTGGAGTAAA
AAGGTTTGGA
TCAGGATTTG
CGCCTTTGGA
TGAGGCACTT
TCCAGAGCGG
TGGTAGATCT
TTCGAACAGG
CCGTACGCAG
TTGTCGAACT
TGGTTTGCAA
AGGGAGAAAG
TAGGAGATCT
CTCTTGCGAG
ATGATCCCGC
ATTTTCTTGA
AAGCTTTGCA
GAGGCTAGCA
GAATTACCCT
CCACGTTGAT
TGTCTGCGAG
GCAAGAATGA
TCATCACCGT
AGTGAGAGTG
CGTTCAAGGC
TCTTGCGGTT
GCCATAAGAG
AAGCCACCTC
GCCCAATGGT
ACCAACGATG
TTCCCTCCAC
CAAAGGTGTT
CTTATGTAGT
TTTACACAGG
AGTCTGGACT
TGACGCTAGT
GATAATAAGT
GACTGAGGTA
TGTGCTCTTC
TTATCTCCTT
TTGTAGTGTT
GCTCTTATTT
TAAACAACTT
TGCGGTTTTT
TGATGACTTT
GCGATTTTGT
TGTTGCTTTG
CAGTAAATTG
CAAGATTTAA
TAAAAAAACG
CAAAGCAATG
ATTAAAGGAT
GTTCAGAATG
AAACTCATGG
AAACACTTAA
CCAGTGCATA
AACGCTGGTC
ATGAAATGAC
GAAGGCTATC
GCCATTGCAC
AGTTTAATGA
TGACAGCCCG
GAAGCGAGGA
AAATAACCCG
GCGCTGGAGA
ATAGGTGAAG
CAGCGGATTT
AGTTGGGGTT
TCTTCTCAGG
CTATCAGAGA
TGCCGAGAAA
GCAGGGCGAC
TACCGCACCC
GGATATGGAA
ATTCGAGGAC
GGGTTGAGCA
ACGTGTTGGT
TATACAATTG
AACAAATTAA
TCATATGCGT
GATGTGTTTG
GTACGCGATT
GCGACGTGCT
GAAGACGTAT
TTCCACCGGT
GATCGGGGTT
GCTGCCCATA
AAGGTGGCGT
TTACAAAACC
TCAGTTTCTG
TTCATCTTGC
TCAGGATCTG
GCTCTGAAGG
GGCTACGTGT
TTTGCTCGTG
GAAGGTAACG
ACCCCCAGGG
AACAGCCTCA
ATGTATCACG
GATGGGTACC
AGATCTTCAT
ATTCATGCAG
AAGACACTCT
CCTGCCTTTC
TATCTTGGGG
AAAAGGACGA
TGTCACTTAT
GCAATAAAGC
CCACTTGCTG
GCCGGGGCTT
GACATTATTC
CTTCCTGTCT
GGCTCTGCAC
CGTATTGAAA
CTGAGTTAAT
GGGCAAATTT
GATGAAGGTA
AACTGCCCAC
CGATCCACAC
CTGATGCTCC
GACTGGCCAT
TGAAACTGTT
GCTCATGACT
ATGATGTCAT
AGTTATTGAC
AGCGCGCCTA
ACCTGGGTAT
CGGCACGATT
AATGTCGTAT
GTGCTGCTGA
TGTGCTGATT
GTTCCCACGC
CTGCTGAGTT
GTTTGACTAC
ACCTCCGCAC
TGCAGTTTTT
CGATATGCTT
CGTGATCTGC
TCAAGAACGT
TGATCTTAAA
GGGTTCGAGC
CTGATGTACG
TATTTTGCTT
ACCAAATACA
GCAATAGCAA
TGGCTCTCAG
TCCCCGTGGA
TGGAGGAGCA
AATTCGGGAT
GCCTGGGGAA
GCATGGTTCT
AAAAAATGTT
GTACGTGAAA
CGGATGAAGT
TGGTAAAGGT
CAGATCCGGA
TGAGAACTGT
TTTTGAACAG
GCCATTGATC
AACGCTCTTC
AACTGGTGCC
TGGAGAAATG
CTCTTTCTAT
TTGGGAACCT
GTCTGCAATG
AAATTTTCGA
TCGTCTGATT
AAACCACGCT
GGGAGATTAG
ATAATGAAGC
GTGCGCCTGT
TATTCCAAAA
CATACGCTCA
ATACTCAACC
GGTTGAAGAT
ACTTCGTTAT
CGACACCAGC
TGCCCCGATG
GTGGATTCGT
TAATTGCGCG
CGTAGGAGTA
ATGGCTCGCG
GTAATGCCAT
TACTTTGCCT
GTATGTGGTC
GGGATGTGAA
GTTTACTCTT
GAAGTGCTCC
GGGGTGATAG
TGTTGAGAAG
ACCTCTCGGG
TATGGTCAGG
TAATGAACGT
GACCAGGAGC
TGCTTACTGA
GGACGCACTG
GATGATCTCA
TCCCTTCTTT
TCTACTGACT
GGTCAACAGA
CACCGGCGTT
CGGTCGAAGA
GTATCTGGTG
TCATAGAAAT
TGCCGATGGG
AGTCGCCGTC
GTAAAGCTGC
TGCACTTACC
GAAAGTGATT
ATCGTGTTCT
GGTTGGCGAG
CTGGATGATG
AGCAGATGGC
TGCATTATCC
AGATTGGGTA
ACGATTATCG
CCCAACAAGT
GCTTATGAAC
GTGGTCAGCG
TTATGCAAGC
CGATTGCAGA
ATGAATTTGC
TGGAAATATT
TCTGCGCTGG
CTGATGCGGA
AAATATTTCA
CGTAAGATTA
TTACCCGCTG
TATCAACACC
GCCAAATTGC
CTAAATCAGT
TGTTGCTCTT
TTTTCTCACC
CCGGTGAACT
ATCTGCCCGG
TCAGGTGATG
CACTTCAAAA
AGCCTTTACA
GATAAAGAGG
AATTACTTAA
GCAGCAGGCA
TCTAACCTTC
ATGAGCAGAA
AAAAGCTGGG
GTGATATTTG
AAGCTGAAGA
AGTTATCACT
CTTTTAACTT
CTGTGCTTAA
AACGTCATCT
GCATCAAGAA
CTAGTTTAAG
CTCACGACAT
CAGTTTGCTC
CTGGAGCGAC
AGTATTGTAT
AAGGGCGATA
AAATGGTGCT
TAACCTGGAC
AGGTCTCGTG
TTCCAACTGA
GTGTATAGAG
AAAATTGAGG
CCATTCTTAA
GGAACTTGAA
AAGCCAGCAC
CCTGATGCGA
CCTCGTTTTA
GTCTACGTTT
ATCTGTCTTT
ACTTAATGTC
CTTTGTTACA
GGCCAGAAAG
CATAACTGGC
CTGAATATTC
TCTCTGGGCC
CACTGTTCCA
CTTGTATCGT
CGGTCTGATA
ATCAGACTGG
GACCACGGTC
CCACTCGTAT
CGTCGGTCTG
ATTATTAGTC
TGGGACCACG
GTCCCACTCG
TATCGTCGGT
CTGATTATTA
GTCTGGGACC
ACGGTCCCAC
TCGTATCGTC
GGTCTGATAA
TCAGACTGGG
ACCACGGTCC
CACTCGTATC
GTCGGTCTGA
TTATTAGTCT
GGGACCATGG
TCCCACTCGT
ATCGTCGGTC
TGATTATTAG
TCTGGGACCA
CGGTCCCACT
CGTATCGTCG
GTCTGATTAT
TAGTCTGGAA
CCACGGTCCC
ACTCGTATCG
TCGGTCTGAT
TATTAGTCTG
GGACCACGGT
CCCACTCGTA
TCGTCGGTCT
GATTATTAGT
CTGGGACCAC
GATCCCACTC
GTGTTGTCGG
TCTGATTATC
GGTCTGGGAC
CACGGTCCCA
CTTGTATTGT
CGATCAGACT
ATCAGCGTGA
GACTACGATT
CCATCAATGC
CTGTCAAGGG
CAAGTATTGA
CATGTCGTCG
TAACCTGTAG
AACGGAGTAA
CCTCGGTGTG
CGGTTGTATG
CCTGCTGTGG
ATTGCTGCTG
TGTCCTGCTT
ATCCACAACA
TTTTGCGCAC
GGTTATGTGG
ACAAAATACC
TGGTTACCCA
GGCCGTGCCG
GCACGTTAAC
CGGGCTGCAT
CCGATGCAAG
TGTGTCGCTG
TCGACGAGCT
CGCGAGCTCG
GACATGAGGT
TGCCCCGTAT
TCAGTGTCGC
TGATTTGTAT
TGTCTGAAGT
TGTTTTTACG
TTAAGTTGAT
GCAGATCAAT
TAATACGATA
CCTGCGTCAT
AATTGATTAT
TTGACGTGGT
TTGATGGCCT
CCACGCACGT
TGTGATATGT
AGATGATAAT
CATTATCACT
TTACGGGTCC
TTTCCGGTGA
TCCGACAGGT
TACGGGGCGG
CGACCTCGCG
GGTTTTCGCT
ATTTATGAAA
ATTTTCCGGT
TTAAGGCGTT
TCCGTTCTTC
TTCGTCATAA
CTTAATGTTT
TTATTTAAAA
TACCCTCTGA
AAAGAAAGGA
AACGACAGGT
GCTGAAAGCG
AGCTTTTTGG
CCTCTGTCGT
TTCCTTTCTC
TGTTTTTGTC
CGTGGAATGA
ACAATGGAAG
TCCGAGCTCA
TCGCTAATAA
CTTCGTATAG
CATACATTAT
ACGAAGTTAT
ATTCGATGCG
GCCGCAAGGG
GTTCGCGTCA
GCGGGTGTTG
GCGGGTGTCG
GGGCTGGCTT
AACTATGCGG
CATCAGAGCA
GATTGTACTG
AGAGTGCACC
ATATGCGGTG
TGAAATACCA
CACAGATGCG
TAAGGAGAAA
ATACCGCATC
AGGCGCCATT
CGCCATTCAG
CTGCGCAACT
GTTGGGAAGG
GCGATCGGTG
CGGGCCTCTT
CGCTATTACG
CCAGCTGGCG
AAAGGGGGAT
GTGCTGCAAG
GCGATTAAGT
TGGGTAACGC
CAGGGTTTTC
CCAGTCACGA
CGTTGTAAAA
CGACGGCCAG
TGAATTGTAA
TACGACTCAC
TATAGGGCGA
ATTCGAGCTC
GGTACCCGGG
GATCCTCTAG
AGTCGACCTG
CAGGCATGCA
AGCTTGAGTA
TTCTATAGTC
TCACCTAAAT
AGCTTGGCGT
AATCATGGTC
ATAGCTGTTT
CCTGTGTGAA
ATTGTTATCC
GCTCACAATT
CCACACAACA
TACGAGCCGG
AAGCATAAAG
TGTAAAGCCT
GGGGTGCCTA
ATGAGTGAGC
TAACTCACAT
TAATTGCGTT
GCGCTCACTG
CCCGCTTTCC
AGTCGGGAAA
CCTGTCGTGC
CAGCTGCATT
AATGAATCGG
CCAACGCGAA
CCCCTTGCGG
CCGCCCGGGC
CGTCGACCAA
TTCTCATGTT
TGACAGCTTA
TCATCGAATT
TCTGCCATTC
ATCCGCTTAT
TATCACTTAT
TCAGGCGTAG
CAACCAGGCG
TTTAAGGGCA
CCAATAACTG
CCTTAAAAAA
ATTACGCCCC
GCCCTGCCAC
TCATCGCAGT
ACTGTTGTAA
TTCATTAAGC
ATTCTGCCGA
CATGGAAGCC
ATCACAAACG
GCATGATGAA
CCTGAATCGC
CAGCGGCATC
AGCACCTTGT
CGCCTTGCGT
ATAATATTTG
CCCATGGTGA
AAACGGGGGC
GAAGAAGTTG
TCCATATTGG
CCACGTTTAA
ATCAAAACTG
GTGAAACTCA
CCCAGGGATT
GGCTGAGACG
AAAAACATAT
TCTCAATAAA
CCCTTTAGGG
AAATAGGCCA
GGTTTTCACC
GTAACACGCC
ACATCTTGCG
AATATATGTG
TAGAAACTGC
CGGAAATCGT
CGTGGTATTC
ACTCCAGAGC
GATGAAAACG
TTTCAGTTTG
CTCATGGAAA
ACGGTGTAAC
AAGGGTGAAC
ACTATCCCAT
ATCACCAGCT
CACCGTCTTT
CATTGCCATA
CGAAATTCCG
GATGAGCATT
CATCAGGCGG
GCAAGAATGT
GAATAAAGGC
CGGATAAAAC
TTGTGCTTAT
TTTTCTTTAC
GGTCTTTAAA
AAGGCCGTAA
TATCCAGCTG
AACGGTCTGG
TTATAGGTAC
ATTGAGCAAC
TGACTGAAAT
GCCTCAAAAT
GTTCTTTACG
ATGCCATTGG
GATATATCAA
CGGTGGTATA
TCCAGTGATT
TTTTTCTCCA
TTTTAGCTTC
CTTAGCTCCT
GAAAATCTCG
ATAACTCAAA
AAATACGCCC
GGTAGTGATC
TTATTTCATT
ATGGTGAAAG
TTGGAACCTC
TTACGTGCCG
ATCAACGTCT
CATTTTCGCC
AAAAGTTGGC
CCAGGGCTTC
CCGGTATCAA
CAGGGACACC
AGGATTTATT
TATTCTGCGA
AGTGATCTTC
CGTCACAGGT
ATTTATTCGC
GATAAGCTCA
TGGAGCGGCG
TAACCGTCGC
ACAGGAAGGA
CAGAGAAAGC
GCGGATCTGG
GAAGTGACGG
ACAGAACGGT
CAGGACCTGG
ATTGGGGAGG
CGGTTGCCGC
CGCTGCTGCT
GACGGTGTGA
CGTTCTCTGT
TCCGGTCACA
CCACATACGT
TCCGCCATTC
CTATGCGATG
CACATGCTGT
ATGCCGGTAT
ACCGCTGAAA
GTTCTGCAAA
GCCTGATGGG
ACATAAGTCC
ATCAGTTCAA
CGGAAGTCTA
CACGAAGGTT
TTTGCGCTGG
ATGTGGCTGC
CCGGCACCGG
GTGCAGTTTG
CGATGCCGGA
GTCTGATGCG
GTTGCGATGC
TGAAACAATT
ATCCTGAGAA
TAAATGCCTT
GGCCTTTATA
TGGAAATGTG
GAACTGAGTG
GATATGCTGT
TTTTGTCTGT
TAAACAGAGA
AGCTGGCTGT
TATCCACTGA
GAAGCGAACG
AAACAGTCGG
GAAAATCTCC
CATTATCGTA
GAGATCCGCA
TTATTAATCT
CAGGAGCCTG
TGTAGCGTTT
ATAGGAAGTA
GTGTTCTGTC
ATGATGCCTG
CAAGCGGTAA
CGAAAACGAT
TTGAATATGC
CTTCAGGAAC
AATAGAAATC
TTCGTGCGGT
GTTACGTTGA
AGTGGAGCGG
ATTATGTCAG
CAATGGACAG
AACAACCTAA
TGAACACAGA
ACCATGATGT
GGTCTGTCCT
TTTACAGCCA
GTAGTGCTCG
CCGCAGTCGA
GCGACAGGGC
GAAGCCCACC
ATGATTACGC
CAAGCTCGAA
ATTAACCCTC
ACTAAAGGGA
ACAAAAGCTG
GTACCTAACA
GGATTAGTGC
AATTCGAGTT
GAATCACTGG
GAAAAACATT
GTCTTCTTTT
TTATATTATC
ATTTGCATTA
GTGCTGCAGT
CGTAGATACT
TGTTGGTTGA
AAGACATCAG
CTGGGAGGGA
CTGGACTAGC
GTTTGGTAAG
GAGACATACC
TGTTAACGTT
GGTTGCAAAA
TTCCATTTCG
CGATTTATGT
TATCTGTAAA
TCCTGATTTG
TCTGGAATTC
TTGATACTTC
CGTTTTTTTA
GAGGCCAATG
ATTAGCATCG
GCGATTCTCA
AAATAGCATT
TTCGACATGC
GGTGCTGATT
TCATAAACAT
AGACAACGCT
TTTACATGTA
AAAGTAACTT
GCGGACTTGG
AACAGTGCTC
TGTTTTTGGT
GTGAACGTAA
CTCAGCAATA
TTTCTGTGCT
AGCAAGGTTT
TTTATGATCG
ACCGAAGATC
TCAAAACTCC
GGGTCTTTCA
ACTGTCTGAC
TAGACCATGT
TCGTAACGTC
GGACAGCAGC
TTTCGTTGTA
CTGGTAGAAT
TTCTACGTGC
GAAGCACGTG
TAGGCAGGTT
GAACGACGAT
CCCTGCCGAT
GGATGGATTG
GCACGCGGCG
GAACGCTTTC
GTGATCTACA
CCACCTGGAT
CTTCACATAT
CTTCGAAATC
GAAAAATTAA
CCAAGTCGAC
GGTATCGATA
ATATTCTAGC
TGAGGGTACC
CATGGCCAAG
TTGACCAGTG
CCGTTCCGGT
GCTCACCGCG
CGCGACGTCG
CCGGAGCGGT
CGAGTTCTGG
ACCGACCGGC
TCGGGTTCTC
CCGGGACTTC
GTGGAGGACG
ACTTCGCCGG
TGTGGTCCGG
GACGACGTGA
CCCTGTTCAT
CAGCGCGGTC
CAGGACCAGG
TGGTGCCGGA
CAACACCCTG
GCCTGGGTGT
GGGTGCGCGG
CCTGGACGAG
CTGTACGCCG
AGTGGTCGGA
GGTCGTGTCC
ACGAACTTCC
GGGACGCCTC
CGGGCCGGCC
ATGACCGAGA
TCGGCGAGCA
GCCGTGGGGG
CGGGAGTTCG
CCCTGCGCGA
CCCGGCCGGC
AACTGCGTGC
ACTTCGTGGC
CGAGGAGCAG
GACTGACCGA
CGCCGACCAA
CACCGCCGGT
CCGACGCGGC
CCGACGGGTC
CGAGGCCTCG
GAGATCTGGG
CCCATGCGGC
CGCAACAACT
ACCTCGACTT
TGGCTGGGAC
ACTTTCAGTG
AGGACAAGAA
GCTTCAGAAG
CGTGCTATCG
AACTCAACCA
GGGACGTGCG
GCACAAATGG
GCATCCTTGC
TCTCATGGTG
CACGAACAGT
TGGGAGTCTC
TATCCTTCCT
TAAAAATTTA
ATTTTCATTA
GTTGCAGTCA
CTCCGCTTTG
GTTTCACAGT
CAGGAATAAC
ACTAGCTCGT
CTTCACGTAA
CTATAACGGT
CCTAAGGTAG
CGAACGTTGC
AGGCCATGCT
GTCCAGGCAG
GTAGATGACG
ACCATCAGGG
ACAGCTTCAA
GGATCGCTCG
CGGCTCTTAC
CAGCCTAACT
TCGATCATTG
GACCGCTGAT
CGTCACGGCG
ATTTATGCCG
CCTCGGCGAG
CACATGGAAC
GGGTTGGCAT
GGATTGTAGG
CGCCGCCCTA
TACCTTGTCT
GCCTCCCCGC
GTTGCGTCGC
GGTGCATGGA
GCCGGGCCAC
CTCGACCTGA
ATGGAAGCCG
GCGGCACCTC
GCTAACGGAT
TCACCACTCC
AAGAATTGGA
GCCAATCAAT
TCTTGCGGAG
AACTGTGAAT
GCGCAAACCA
ACCCTTGGCA
GAACATATCC
ATCGCGTCCG
CCATCTCCAG
CAGCCGCACG
CGGCGCATCG
GGGGGGGGGG
GGTTTCAATT
CATCATTTTT
TTTTTATTCT
TTTTTTTGAT
TTCGGTTTCC
TTGAAATTTT
TTTGATTCGG
TAATCTCCGA
ACAGAAGGAA
GAACGAAGGA
AGGAGCACAG
ACTTAGATTG
GTATATATAC
GCATATGTAG
TGTTGAAGAA
ACATGAAATT
GCCCAGTATT
CTTAACCCAA
CTGCACAGAA
CAAAAACCTG
CAGGAAACGA
AGATAAATCA
TGTCGAAAGC
TACATATAAG
GAACGTGCTG
CTACTCATCC
TAGTCCTGTT
GCTGCCAAGC
TATTTAATAT
CATGCACGAA
AAGCAAACAA
ACTTGTGTGC
TTCATTGGAT
GTTCGTACCA
CCAAGGAATT
ACTGGAGTTA
GTTGAAGCAT
TAGGTCCCAA
AATTTGTTTA
CTAAAAACAC
ATGTGGATAT
CTTGACTGAT
TTTTCCATGG
AGGGCACAGT
TAAGCCGCTA
AAGGCATTAT
CCGCCAAGTA
CAATTTTTTA
CTCTTCGAAG
ACAGAAAATT
TGCTGACATT
GGTAATACAG
TCAAATTGCA
GTACTCTGCG
GGTGTATACA
GAATAGCAGA
ATGGGCAGAC
ATTACGAATG
CACACGGTGT
GGTGGGCCCA
GGTATTGTTA
GCGGTTTGAA
GCAGGCGGCG
GAAGAAGTAA
CAAAGGAACC
TAGAGGCCTT
TTGATGTTAG
CAGAATTGTC
ATGCAAGGGC
TCCCTAGCTA
CTGGAGAATA
TACTAAGGGT
ACTGTTGACA
TTGCGAAGAG
CGACAAAGAT
TTTGTTATCG
GCTTTATTGC
TCAAAGAGAC
ATGGGTGGAA
GAGATGAAGG
TTACGATTGG
TTGATTATGA
CACCCGGTGT
GGGTTTAGAT
GACAAGGGAG
ACGCATTGGG
TCAACAGTAT
AGAACCGTGG
ATGATGTGGT
CTCTACAGGA
TCTGACATTA
TTATTGTTGG
AAGAGGACTA
TTTGCAAAGG
GAAGGGATGC
TAAGGTAGAG
GGTGAACGTT
ACAGAAAAGC
AGGCTGGGAA
GCATATTTGA
GAAGATGCGG
CCAGCAAAAC
TAAAAAACTG
TATTATAAGT
AAATGCATGT
ATACTAAACT
CACAAATTAG
AGCTTCAATT
TAATTATATC
AGTTATTACC
CGGCCGGGAA
TCTCGGTCGT
AATGATTTTT
ATAATGACGA
AAAAAAAAAA
AATTGGAAAG
AAAACCCCCC
CCCCCCCGCA
GCGTTGGGTC
CTGGCCACGG
GTGCGCATGA
TCGTGCTCCT
GTCGTTGAGG
ACCCGGCTAG
GCTGGCGGGG
TTGCCTTACT
GGTTAGCAGA
ATGAATCACC
GATACGCGAG
CGAACGTGAA
GCGACTGCTG
CTGCAAAACG
TCTGCGACCT
GAGCAACAAC
ATGAATGGTC
TTCGGTTTCC
GTGTTTCGTA
AAGTCTGGAA
ACGCGGAAGT
CAGCGCCCTG
CACCATTATG
TTCCGGATCT
GCATCGCAGG
ATGCTGCTGG
CTACCCTGTG
GAACACCTAC
ATCTGTATTA
ACGAAGCGCT
GGCATTGACC
CTGAGTGATT
TTTCTCTGGT
CCCGCCGCAT
CCATACCGCC
AGTTGTTTAC
CCTCACAACG
TTCCAGTAAC
CGGGCATGTT
CATCATCAGT
AACCCGTATC
GTGAGCATCC
TCTCTCGTTT
CATCGAGTTA
CGCTAGGGAT
AACAGGTAAT
ATAGCATCTT
CCGCTGCATA
ACCCTGCTTC
GGGGTCATTA
TAGCGATTTT
TTCGGTATAT
CCATCCTTTT
TCGCACGATA
TACAGGATTT
TGCCAAAGGG
TTCGTGTAGA
CTTTCCTTGG
TGTATCCAAC
GGCGTCAGCC
GGGCAGGATA
GGTGAAGTAG
GCCCACCCGC
GAGCGGGTGT
TCCTTCTTCA
CTGTCCCTTA
TTCGCACCTG
GCGGTGCTCA
ACGGGAATCC
TGCTCTGCGA
GGCTGGCCGG
CTACCGCCGG
CGTAACAGAT
GAGGGCAAGC
GGATGGCTGA
TGAAACCAAG
CCAACCAGGA
AGGGCAGCCC
ACCTATCAAG
GTGTACTGCC
TTCCAGACGA
ACGAAGAGCG
ATTGAGGAAA
AGGCGGCGGC
GGCCGGCATG
AGCCTGTCGG
CCTACCTGCT
GGCCGTCGGC
CAGGGCTACA
AAATCACGGG
CGTCGTGGAC
TATGAGCACG
TCCGCGAGCT
GGCCCGCATC
AATGGCGACC
TGGGCCGCCT
GGGCGGCCTG
CTGAAACTCT
GGCTCACCGA
CGACCCGCGC
ACGGCGCGGT
TCGGTGATGC
CACGATCCTC
GCCCTGCTGG
CGAAGATCGA
AGAGAAGCAG
GACGAGCTTG
GCAAGGTCAT
GATGGGCGTG
GTCCGCCCGA
GGGCAGAGCC
ATGACTTTTT
TAGCCGCTAA
AACGGCCGGG
GGGTGCGCGT
GATTGCCAAG
CACGTCCCCA
TGCGCTCCAT
CAAGAAGAGG
CACTTCGAGC
TGTAAGTACA
TCACCGACGA
GCAAGGCAAG
ACGATCCGCG
CCTGTTTTAT
TGAGAACGTT
GTTCGTGTTG
GCCTCAATGG
TAGCGATGCG
TCATTCAGCG
AAGTTCTGGT
GCTGATGATG
TGGTTCGCTT
TGCCACTGGT
CAATGTGGTA
AGCCCGTGTA
ATGTCAGTAA
CCTTTTTACT
GATCTCAGCT
TGAGCACGGT
CGCTGATGAG
CTTATCCATG
GCCCCACGGT
AACGGATATG
ATCCTCTAGG
GCGTTGACAA
ATTCTTTGTC
GGTTTTCATG
GGGTAGACGT
CAGTGACCAG
GGAACGTCTC
CCTACAAAAA
GTTGCGCATA
CTTTGCTCCG
TTGTCGACGG
CAGGGGTGTC
GGACCAGATT
GTATCTGTGG
CAACGGCCTC
ATTGCGTCAA
TGGACCTTTA
AAGCCGGGAA
ACGAGACTTG
AAATGTTTAC
GTAGAGGTGC
ATTATAGACC
TCACGCGCAT
ATTGAGTGGT
TGCAAAAATG
GTTTTGCGAA
CGGTATCACT
GGAGACCCAT
GCCAGGCAAG
GACGGAGGGC
ATCATAATCA
TGTTCATTGC
GTTGGACAGC
ATGCTTGTTA
CACGTAAGTA
TACGGTCGAG
AGCATCGTGA
CGTTGAGAAT
TACAGATGGC
AACGTGTCGG
TGCATGTATT
GGCCGAGATT
GTCAAATCGT
GGCTCAATAT
ATGGCATGCC
AGGTAAGTCA
TGGATGTCGG
TATGCCATTC
AGCCTTCATG
TCGATTTCGT
TGTCGAGAAC
TGATGGGTCC
CAGTCAACGT
CAGAAGTAAG
AACAACATGG
GGAAGGGAAT
GAAGTTCATG
ACTGGTGGGT
GCGCGCATAT
CCATGTACGG
AAGACCCTGT
CGTATGTTTA
GTGGGATGAT
GTAATCATCC
AGGGTAACAA
TACGTTGGTT
GCCGCCCACG
GTACGAGACC
GATCTTGAAC
ACAATTGTGG
CAATGTTCAA
GTTGGGCGCT
GGAATGGATG
GTTTTACCTT
TACCAAGATG
CGCGTATTGA
TGCATGATAA
CAACAATGGG
CCCTCGTTGC
GATTCGACAA
GACCGGCAGC
CGTGACAATG
TCTAGATTGG
ACAAGGTATG
TTCATTTATA
CCGGTTATGT
TGGCTGAACG
TCCAGTTTTG
TGCAGTACGG
TAACATCACT
CCCCGCAAGT
CCACCGTTGG
CACCGCGATC
GACAAGTGCA
GATGTGGTAT
TTTGTACTGT
GTGTCGGGAG
ACTTGGTACT
GTAACACATT
TGACTGAAGT
GACTTGGTGG
TACCATGAGG
ACGGAGGGAA
CCCGTTGATA
CGGTGTTGCG
TGACGCTGCA
AGTACCTTAC
GTATGTCGCC
TGGATCCATA
CGTCCAACCC
GATCAGAAAG
ATGTGCTAGG
AGTTCCGTTT
CTGGTGCACA
ATCATGAAAG
GTATCCACGG
GAGTTCCATT
GTTGTCGTCC
GGGGCTGCGT
CGCGACTGCC
AGTTGCATGT
ACATGCGCCA
TCAAGGGACG
TGCCAATCCA
TTACTAACCG
GGAGAACCTT
AGCTGGGGCA
GCCAATCCCT
GAAGTATAGC
CTTAGCATCA
TCTGAGAGCT
GGTTCCACAT
ATCTCTAGGG
ATATAAGGTC
GTTCACGGTT
AGTGGGCGTA
CTATGATGTC
GGTTATTAGT
ACAATTCTCC
CTGCGGGCAT
GCGCATTGGC
TTCATAGAGT
ACGGAAGGTG
ACAAGTCAAT
ATTGTAGTTA
ACATCCGGAT
CAGTGTCAAA
GTCTGTAGGA
TGGTAAGAAA
GATCAGTAGA
ATGAATACTA
CGCTTTTCCT
TGGGACTACG
GGAATTAGAG
AAGTTGTTTC
CTTTATTGTA
GAGTGACGCT
GAAGCAAGTA
GAAGACTAAG
GTAACTCTCG
TAGCTAATAG
GATTACCTCC
TTTGGCTAGG
TCAAGAGTGG
CTGTGATCTT
CACTTGACAA
AGTTCCGGTA
CATTGTGGAC
AGCATTCTCC
AAAAGACTAA
GACACAGTTG
CTTTGGGAGT
TGCTCAGCCA
TTGGTACAGT
ATTGTGGTAG
ATACAAAGGT
GGTTCTTCCA
ATGAAGGATA
AATCCTTCTG
CTGTGCCTGT
CCATGAGGAT
CCATATTTCG
CCGTAGTTAG
GTAACCAAGC
GTGGTGGCTG
AACTGATCTT
CGCACTTGCT
GATTCCGTAT
AGTGTTTGAC
AACTTTACAA
AACACTTCTT
GCGCAGTTCG
CTCTAGCCTG
GACAGAGAAG
GGACAATCTT
CGTCGTTAAG
ACTCGTGCGA
ATAGCAAAAG
ATCACAAAAT
AGCACATCGG
CACCGACCAA
CGATTATTTC
CAAGGAAAAA
AAGAATGCTT
CACTACAAGA
AATTGTGTCA
TCCCTATACA
GAGTCTTGTT
ACTGTGACAG
AAAATTGATG
GAAGATGTGG
CGGATTGCCT
TTACACTAGC
CAACTTGTTC
GACTAATTGC
AGCTTCTTCT
GAGAGGCTTC
ACCGAGTAAC
GCGAAGAACA
CCGGTGTCTC
GTACATGCTC
GTCGGTGAAC
GCTCGTCCAA
TGACACCCCC
CACTTTGTAT
CAATATCCCA
ACTTGGTAGT
GAACTGGAAT
GATACATGCA
ATTTCGCGCC
GCATCAACAG
CCACGGGCCA
CCATCGACGA
ATAGACTCGG