名称: | pHAGE UbiC scAB-GFP |
---|---|
别称: | Plasmid#104998 |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 蛋白表达,抗体表达 |
片段类型: | ORF |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | 绿色 |
启动子: | UbiC |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
GGCCTCCGCG
CCGGGTTTTG
GCGCCTCCCG
CGGGCGCCCC
CCTCCTCACG
GCGAGCGCTG
CCACGTCAGA
CGAAGGGCGC
AGCGAGCGTC
CTGATCCTTC
CGCCCGGACG
CTCAGGACAG
CGGCCCGCTG
CTCATAAGAC
TCGGCCTTAG
AACCCCAGTA
TCAGCAGAAG
GACATTTTAG
GACGGGACTT
GGGTGACTCT
AGGGCACTGG
TTTTCTTTCC
AGAGAGCGGA
ACAGGCGAGG
AAAAGTAGTC
CCTTCTCGGC
GATTCTGCGG
AGGGATCTCC
GTGGGGCGGT
GAACGCCGAT
GATTATATAA
GGACGCGCCG
GGTGTGGCAC
AGCTAGTTCC
GTCGCAGCCG
GGATTTGGGT
CGCGGTTCTT
GTTTGTGGAT
CGCTGTGATC
GTCACTTGGC
GGCCATGGGC
CCAGATATTG
TTATGACCCA
GTCCCCGTCC
TCCCTGTCCG
CAAGCGTTGG
CGACCGTGTG
ACCATTACCT
GCCGTTCCTC
CACCGGCGCT
GTGACCACCA
GCAACTACGC
CAGCTGGGTG
CAGGAGAAGC
CGGGCAAACT
GTTCAAGGGT
CTGATCGGCG
GTACCAATAA
CCGTGCCCCG
GGTGTGCCAA
GCCGCTTTAG
CGGCAGCCTG
ATCGGTGACA
AAGCTACCCT
GACCATTAGC
AGCCTGCAGC
CGGAGGATTT
CGCCACCTAC
TTTTGCGCGC
TGTGGTATAG
CAACCACTGG
GTGTTCGGCC
AGGGTACCAA
AGTGGAACTG
AAGCGTGGCG
GTGGGGGTTC
TGGAGGTGGT
GGGAGTGGCG
GTGGCGGTTC
TAGCGGAGGG
GGTTCCGAGG
TGAAACTGCT
GGAGAGCGGT
GGCGGTCTGG
TGCAGCCGGG
CGGTAGCCTG
AAGCTGAGCT
GCGCCGTGAG
CGGCTTCAGC
CTGACCGACT
ACGGTGTGAA
CTGGGTGCGT
CAGGCCCCAG
GTCGCGGTCT
GGAATGGATC
GGCGTGATTT
GGGGCGACGG
TATTACCGAT
TATAACAGCG
CCCTGAAGGA
CCGTTTTATC
ATTAGCAAAG
ATAACGGCAA
GAACACCGTG
TACCTGCAGA
TGAGCAAGGT
GCGCAGCGAC
GATACCGCGC
TGTATTATTG
CGTTACCGGC
CTGTTCGACT
ATTGGGGTCA
AGGCACCCTG
GTTACCGTTA
GCAGCTACCC
ATACGATGTT
CCAGATTACG
CTGGTGGAGG
CGGAGGTTCT
GGGGGAGGAG
GTAGTGGCGG
TGGTGGTTCA
GGAGGCGGCG
GAAGCTTGGA
TCCAGGTGGA
GGTGGAAGCG
GTAGCAAAGG
AGAAGAACTT
TTCACTGGAG
TTGTCCCAAT
TCTTGTTGAA
TTAGATGGTG
ATGTTAATGG
GCACAAATTT
TCTGTCCGTG
GAGAGGGTGA
AGGTGATGCT
ACAAACGGAA
AACTCACCCT
TAAATTTATT
TGCACTACTG
GAAAACTACC
TGTTCCGTGG
CCAACACTTG
TCACTACTCT
GACCTATGGT
GTTCAATGCT
TTTCCCGTTA
TCCGGATCAC
ATGAAACGGC
ATGACTTTTT
CAAGAGTGCC
ATGCCCGAAG
GTTATGTACA
GGAACGCACT
ATATCTTTCA
AAGATGACGG
GACCTACAAG
ACGCGTGCTG
AAGTCAAGTT
TGAAGGTGAT
ACCCTTGTTA
ATCGTATCGA
GTTAAAGGGT
ATTGATTTTA
AAGAAGATGG
AAACATTCTT
GGACACAAAC
TCGAGTACAA
CTTTAACTCA
CACAATGTAT
ACATCACGGC
AGACAAACAA
AAGAATGGAA
TCAAAGCTAA
CTTCAAAATT
CGCCACAACG
TTGAAGATGG
TTCCGTTCAA
CTAGCAGACC
ATTATCAACA
AAATACTCCA
ATTGGCGATG
GCCCTGTCCT
TTTACCAGAC
AACCATTACC
TGTCGACACA
ATCTGTCCTT
TCGAAAGATC
CCAACGAAAA
GCGTGACCAC
ATGGTCCTTC
TTGAGTTTGT
AACTGCTGCT
GGGATTACAC
ATGGCATGGA
TGAGCTCTAC
AAAGGTGGAG
GTCGGACCGA
AGAGTACAAG
CTTATCCTGA
ACGGTAAAAC
CCTGAAAGGT
GAAACCACCA
CCGAAGCTGT
TGACGCTGCT
ACCGCGGAAA
AAGTTTTCAA
ACAGTACGCT
AACGACAACG
GTGTTGACGG
TGAATGGACC
TACGACGACG
CTACCAAAAC
CTTCACGGTA
ACCGCATAAT
GAATCGATAG
ATCCTAATCA
ACCTCTGGAT
TACAAAATTT
GTGAAAGATT
GACTGGTATT
CTTAACTATG
TTGCTCCTTT
TACGCTATGT
GGATACGCTG
CTTTAATGCC
TTTGTATCAT
GCTATTGCTT
CCCGTATGGC
TTTCATTTTC
TCCTCCTTGT
ATAAATCCTG
GTTGCTGTCT
CTTTATGAGG
AGTTGTGGCC
CGTTGTCAGG
CAACGTGGCG
TGGTGTGCAC
TGTGTTTGCT
GACGCAACCC
CCACTGGTTG
GGGCATTGCC
ACCACCTGTC
AGCTCCTTTC
CGGGACTTTC
GCTTTCCCCC
TCCCTATTGC
CACGGCGGAA
CTCATCGCCG
CCTGCCTTGC
CCGCTGCTGG
ACAGGGGCTC
GGCTGTTGGG
CACTGACAAT
TCCGTGGTGT
TGTCGGGGAA
ATCATCGTCC
TTTCCTTGGC
TGCTCGCCTG
TGTTGCCACC
TGGATTCTGC
GCGGGACGTC
CTTCTGCTAC
GTCCCTTCGG
CCCTCAATCC
AGCGGACCTT
CCTTCCCGCG
GCCTGCTGCC
GGCTCTGCGG
CCTCTTCCGC
GTCTTCGCCT
TCGCCCTCAG
ACGAGTCGGA
TCTCCCTTTG
GGCCGCCTCC
CCGCCTGAGA
TCCTTTAAGA
CCAATGACTT
ACAAGGCAGC
TGTAGATCTT
AGCCACTTTT
TAAAAGAAAA
GGGGGGACTG
GAAGGGCTAA
TTCACTCCCA
ACGAAGACAA
GATCTGCTTT
TTGCTTGTAC
TGGGTCTCTC
TGGTTAGACC
AGATCTGAGC
CTGGGAGCTC
TCTGGCTAAC
TAGGGAACCC
ACTGCTTAAG
CCTCAATAAA
GCTTGCCTTG
AGTGCTTCAA
GTAGTGTGTG
CCCGTCTGTT
GTGTGACTCT
GGTAACTAGA
GATCCCTCAG
ACCCTTTTAG
TCAGTGTGGA
AAATCTCTAG
CAGTAGTAGT
TCATGTCATC
TTATTATTCA
GTATTTATAA
CTTGCAAAGA
AATGAATATC
AGAGAGTGAG
AGGCCCGGGT
TAATTAAGGA
AAGGGCTAGA
TCATTCTTGA
AGACGAAAGG
GCCTCGTGAT
ACGCCTATTT
TTATAGGTTA
ATGTCATGAT
AATAATGGTT
TCTTAGACGT
CAGGTGGCAC
TTTTCGGGGA
AATGTGCGCG
GAACCCCTAT
TTGTTTATTT
TTCTAAATAC
ATTCAAATAT
GTATCCGCTC
ATGAGACAAT
AACCCTGATA
AATGCTTCAA
TAATATTGAA
AAAGGAAGAG
TATGAGTATT
CAACATTTCC
GTGTCGCCCT
TATTCCCTTT
TTTGCGGCAT
TTTGCCTTCC
TGTTTTTGCT
CACCCAGAAA
CGCTGGTGAA
AGTAAAAGAT
GCTGAAGATC
AGTTGGGTGC
ACGAGTGGGT
TACATCGAAC
TGGATCTCAA
CAGCGGTAAG
ATCCTTGAGA
GTTTTCGCCC
CGAAGAACGT
TTTCCAATGA
TGAGCACTTT
TAAAGTTCTG
CTATGTGGCG
CGGTATTATC
CCGTGTTGAC
GCCGGGCAAG
AGCAACTCGG
TCGCCGCATA
CACTATTCTC
AGAATGACTT
GGTTGAGTAC
TCACCAGTCA
CAGAAAAGCA
TCTTACGGAT
GGCATGACAG
TAAGAGAATT
ATGCAGTGCT
GCCATAACCA
TGAGTGATAA
CACTGCGGCC
AACTTACTTC
TGACAACGAT
CGGAGGACCG
AAGGAGCTAA
CCGCTTTTTT
GCACAACATG
GGGGATCATG
TAACTCGCCT
TGATCGTTGG
GAACCGGAGC
TGAATGAAGC
CATACCAAAC
GACGAGCGTG
ACACCACGAT
GCCTGTAGCA
ATGGCAACAA
CGTTGCGCAA
ACTATTAACT
GGCGAACTAC
TTACTCTAGC
TTCCCGGCAA
CAATTAATAG
ACTGGATGGA
GGCGGATAAA
GTTGCAGGAC
CACTTCTGCG
CTCGGCCCTT
CCGGCTGGCT
GGTTTATTGC
TGATAAATCT
GGAGCCGGTG
AGCGTGGGTC
TCGCGGTATC
ATTGCAGCAC
TGGGGCCAGA
TGGTAAGCCC
TCCCGTATCG
TAGTTATCTA
CACGACGGGG
AGTCAGGCAA
CTATGGATGA
ACGAAATAGA
CAGATCGCTG
AGATAGGTGC
CTCACTGATT
AAGCATTGGT
AACTGTCAGA
CCAAGTTTAC
TCATATATAC
TTTAGATTGA
TTTAAAACTT
CATTTTTAAT
TTAAAAGGAT
CTAGGTGAAG
ATCCTTTTTG
ATAATCTCAT
GACCAAAATC
CCTTAACGTG
AGTTTTCGTT
CCACTGAGCG
TCAGACCCCG
TAGAAAAGAT
CAAAGGATCT
TCTTGAGATC
CTTTTTTTCT
GCGCGTAATC
TGCTGCTTGC
AAACAAAAAA
ACCACCGCTA
CCAGCGGTGG
TTTGTTTGCC
GGATCAAGAG
CTACCAACTC
TTTTTCCGAA
GGTAACTGGC
TTCAGCAGAG
CGCAGATACC
AAATACTGTT
CTTCTAGTGT
AGCCGTAGTT
AGGCCACCAC
TTCAAGAACT
CTGTAGCACC
GCCTACATAC
CTCGCTCTGC
TAATCCTGTT
ACCAGTGGCT
GCTGCCAGTG
GCGATAAGTC
GTGTCTTACC
GGGTTGGACT
CAAGACGATA
GTTACCGGAT
AAGGCGCAGC
GGTCGGGCTG
AACGGGGGGT
TCGTGCACAC
AGCCCAGCTT
GGAGCGAACG
ACCTACACCG
AACTGAGATA
CCTACAGCGT
GAGCTATGAG
AAAGCGCCAC
GCTTCCCGAA
GGGAGAAAGG
CGGACAGGTA
TCCGGTAAGC
GGCAGGGTCG
GAACAGGAGA
GCGCACGAGG
GAGCTTCCAG
GGGGAAACGC
CTGGTATCTT
TATAGTCCTG
TCGGGTTTCG
CCACCTCTGA
CTTGAGCGTC
GATTTTTGTG
ATGCTCGTCA
GGGGGGCGGA
GCCTATGGAA
AAACGCCAGC
AACGCGGCCT
TTTTACGGTT
CCTGGCCTTT
TGCTGGCCTT
TTGCTCACAT
GTTCTTTCCT
GCGTTATCCC
CTGATTCTGT
GGATAACCGT
ATTACCGCCT
TTGAGTGAGC
TGATACCGCT
CGCCGCAGCC
GAACGACCGA
GCGCAGCGAG
TCAGTGAGCG
AGGAAGCGGA
AGAGCGCCCA
ATACGCAAAC
CGCCTCTCCC
CGCGCGTTGG
CCGATTCATT
AATGCAGCAA
GCTCATGGCT
GACTAATTTT
TTTTATTTAT
GCAGAGGCCG
AGGCCGCCTC
GGCCTCTGAG
CTATTCCAGA
AGTAGTGAGG
AGGCTTTTTT
GGAGGCCTAG
GCTTTTGCAA
AAAGCTCCCC
GTGGCACGAC
AGGTTTCCCG
ACTGGAAAGC
GGGCAGTGAG
CGCAACGCAA
TTAATGTGAG
TTAGCTCACT
CATTAGGCAC
CCCAGGCTTT
ACACTTTATG
CTTCCGGCTC
GTATGTTGTG
TGGAATTGTG
AGCGGATAAC
AATTTCACAC
AGGAAACAGC
TATGACATGA
TTACGAATTT
CACAAATAAA
GCATTTTTTT
CACTGCATTC
TAGTTGTGGT
TTGTCCAAAC
TCATCAATGT
ATCTTATCAT
GTCTGGATCA
ACTGGATAAC
TCAAGCTAAC
CAAAATCATC
CCAAACTTCC
CACCCCATAC
CCTATTACCA
CTGCCAATTA
CCTGTGGTTT
CATTTACTCT
AAACCTGTGA
TTCCTCTGAA
TTATTTTCAT
TTTAAAGAAA
TTGTATTTGT
TAAATATGTA
CTACAAACTT
AGTAGTTGGA
AGGGCTAATT
CACTCCCAAA
GAAGACAAGA
TATCCTTGAT
CTGTGGATCT
ACCACACACA
AGGCTACTTC
CCTGATTAGC
AGAACTACAC
ACCAGGGCCA
GGGGTCAGAT
ATCCACTGAC
CTTTGGATGG
TGCTACAAGC
TAGTACCAGT
TGAGCCAGAT
AAGGTAGAAG
AGGCCAATAA
AGGAGAGAAC
ACCAGCTTGT
TACACCCTGT
GAGCCTGCAT
GGGATGGATG
ACCCGGAGAG
AGAAGTGTTA
GAGTGGAGGT
TTGACAGCCG
CCTAGCATTT
CATCACGTGG
CCCGAGAGCT
GCATCCGGAG
TACTTCAAGA
ACTGCTGATA
TCGAGCTTGC
TACAAGGGAC
TTTCCGCTGG
GGACTTTCCA
GGGAGGCGTG
GCCTGGGCGG
GACTGGGGAG
TGGCGAGCCC
TCAGATCCTG
CATATAAGCA
GCTGCTTTTT
GCCTGTACTG
GGTCTCTCTG
GTTAGACCAG
ATCTGAGCCT
GGGAGCTCTC
TGGCTAACTA
GGGAACCCAC
TGCTTAAGCC
TCAATAAAGC
TTGCCTTGAG
TGCTTCAAGT
AGTGTGTGCC
CGTCTGTTGT
GTGACTCTGG
TAACTAGAGA
TCCCTCAGAC
CCTTTTAGTC
AGTGTGGAAA
ATCTCTAGCA
GTGGCGCCCG
AACAGGGACT
TGAAAGCGAA
AGGGAAACCA
GAGGAGCTCT
CTCGACGCAG
GACTCGGCTT
GCTGAAGCGC
GCACGGCAAG
AGGCGAGGGG
CGGCGACTGG
TGAGTACGCC
AAAAATTTTG
ACTAGCGGAG
GCTAGAAGGA
GAGAGATGGG
TGCGAGAGCG
TCAGTATTAA
GCGGGGGAGA
ATTAGATCGC
GATGGGAAAA
AATTCGGTTA
AGGCCAGGGG
GAAAGAAAAA
ATATAAATTA
AAACATATAG
TATGGGCAAG
CAGGGAGCTA
GAACGATTCG
CAGTTAATCC
TGGCCTGTTA
GAAACATCAG
AAGGCTGTAG
ACAAATACTG
GGACAGCTAC
AACCATCCCT
TCAGACAGGA
TCAGAAGAAC
TTAGATCATT
ATATAATACA
GTAGCAACCC
TCTATTGTGT
GCATCAAAGG
ATAGAGATAA
AAGACACCAA
GGAAGCTTTA
GACAAGATAG
AGGAAGAGCA
AAACAAAAGT
AAGACCACCG
CACAGCAAGC
GGCCGGCCGC
TGATCTTCAG
ACCTGGAGGA
GGAGATATGA
GGGACAATTG
GAGAAGTGAA
TTATATAAAT
ATAAAGTAGT
AAAAATTGAA
CCATTAGGAG
TAGCACCCAC
CAAGGCAAAG
AGAAGAGTGG
TGCAGAGAGA
AAAAAGAGCA
GTGGGAATAG
GAGCTTTGTT
CCTTGGGTTC
TTGGGAGCAG
CAGGAAGCAC
TATGGGCGCA
GCGTCAATGA
CGCTGACGGT
ACAGGCCAGA
CAATTATTGT
CTGGTATAGT
GCAGCAGCAG
AACAATTTGC
TGAGGGCTAT
TGAGGCGCAA
CAGCATCTGT
TGCAACTCAC
AGTCTGGGGC
ATCAAGCAGC
TCCAGGCAAG
AATCCTGGCT
GTGGAAAGAT
ACCTAAAGGA
TCAACAGCTC
CTGGGGATTT
GGGGTTGCTC
TGGAAAACTC
ATTTGCACCA
CTGCTGTGCC
TTGGAATGCT
AGTTGGAGTA
ATAAATCTCT
GGAACAGATT
TGGAATCACA
CGACCTGGAT
GGAGTGGGAC
AGAGAAATTA
ACAATTACAC
AAGCTTAATA
CACTCCTTAA
TTGAAGAATC
GCAAAACCAG
CAAGAAAAGA
ATGAACAAGA
ATTATTGGAA
TTAGATAAAT
GGGCAAGTTT
GTGGAATTGG
TTTAACATAA
CAAATTGGCT
GTGGTATATA
AAATTATTCA
TAATGATAGT
AGGAGGCTTG
GTAGGTTTAA
GAATAGTTTT
TGCTGTACTT
TCTATAGTGA
ATAGAGTTAG
GCAGGGATAT
TCACCATTAT
CGTTTCAGAC
CCACCTCCCA
ACCCCGAGGG
GACCCGACAG
GCCCGAAGGA
ATAGAAGAAG
AAGGTGGAGA
GAGAGACAGA
GACAGATCCA
TTCGATTAGT
GAACGGATCT
CGACGGTATC
GCCGAATTCA
CAAATGGCAG
TATTCATCCA
CAATTTTAAA
AGAAAAGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TCGGGTTTAT
TACAGGGACA
GCAGAGATCC
AGTTTGGACT
AGT