名称: | pCS2-6×Myc-NLRP11(human) |
---|---|
别称: | NM_145007.5;CLR19.6; NALP11; NOD17; PAN10; PYPAF6;PYPAF7 |
基因长: | 3099bp |
质粒宿主: | 哺乳细胞,鱼类 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 人 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CGCCATTCTG
CCTGGGGACG
TCGGAGCAAG
CTTGATTTAG
GTGACACTAT
AGAATACAAG
CTACTTGTTC
TTTTTGCAGG
ATCCCATCGA
TTTAAAGCTA
TGGAGCAAAA
GCTCATTTCT
GAAGAGGACT
TGAATGAAAT
GGAGCAAAAG
CTCATTTCTG
AAGAGGACTT
GAATGAAATG
GAGCAAAAGC
TCATTTCTGA
AGAGGACTTG
AATGAAATGG
AGCAAAAGCT
CATTTCTGAA
GAGGACTTGA
ATGAAATGGA
GCAAAAGCTC
ATTTCTGAAG
AGGACTTGAA
TGAAATGGAG
AGCTTGGGCG
ACCTCACCAT
GGAGCAAAAG
CTCATTTCTG
AAGAGGACTT
GAATTCAGCA
GAATCGGATT
CTACTGACTT
TGACCTGCTG
TGGTATCTAG
AGAATCTCAG
TGACAAGGAA
TTTCAGAGTT
TTAAGAAGTA
TCTGGCACGC
AAGATTCTTG
ATTTCAAACT
GCCACAGTTT
CCACTGATAC
AGATGACAAA
AGAAGAACTG
GCTAACGTGT
TGCCAATCTC
TTATGAGGGA
CAGTATATAT
GGAATATGCT
CTTCAGCATA
TTTTCAATGA
TGCGTAAGGA
AGATCTTTGT
AGGAAGATCA
TTGGCAGACG
AAACCGCAAT
CAGGAGGCAT
GCAAAGCTGT
CATGAGGAGA
AAATTCATGC
TGCAATGGGA
AAGTCACACT
TTTGGAAAAT
TTCATTATAA
ATTTTTTCGT
GACGTTTCGT
CAGATGTGTT
CTACATACTT
CAATTAGCCT
ATGATTCTAC
CAGCTATTAT
TCAGCAAACA
ATCTCAATGT
GTTCCTGATG
GGAGAGAGAG
CATCTGGAAA
AACTATTGTT
ATAAATCTGG
CTGTGTTGAG
GTGGATCAAG
GGTGAGATGT
GGCAGAACAT
GATCTCGTAC
GTCGTTCACC
TCACTGCTCA
CGAAATAAAC
CAGATGACCA
ACAGCAGCTT
GGCTGAGCTA
ATCGCCAAGG
ACTGGCCTGA
CGGCCAGGCT
CCCATTGCAG
ACATCCTGTC
TGATCCCAAG
AAACTCCTTT
TCATCCTCGA
GGACTTGGAC
AACATAAGAT
TCGAGTTAAA
TGTCAATGAA
AGTGCTTTGT
GTAGTAACAG
CACCCAGAAA
GTTCCCATTC
CAGTTCTCCT
GGTCAGTTTG
CTGAAGAGAA
AAATGGCTCC
AGGCTGCTGG
TTCCTCATCT
CCTCAAGGCC
CACACGTGGG
AATAATGTAA
AAACGTTCTT
GAAAGAGGTA
GATTGCTGCA
CGACCTTGCA
GCTGTCGAAT
GGGAAGAGGG
AGATATATTT
TAACTCTTTC
TTTAAAGACC
GCCAGAGGGC
GTCGGCAGCC
CTCCAGCTTG
TACATGAGGA
TGAAATACTC
GTGGGTCTGT
GCCGAGTCGC
CATCTTATGC
TGGATCACGT
GTACTGTCCT
GAAGCGGCAG
ATGGACAAGG
GGCGTGACTT
CCAGCTCTGC
TGCCAAACAC
CCACTGATCT
ACATGCCCAC
TTTCTTGCTG
ATGCGTTGAC
ATCAGAGGCT
GGACTTACTG
CCAATCAGTA
TCACCTAGGT
CTCCTAAAAC
GTCTGTGTTT
GCTGGCTGCA
GGAGGACTGT
TTCTGAGCAC
CCTGAATTTC
AGTGGTGAAG
ACCTCAGATG
TGTTGGGTTT
ACTGAGGCTG
ATGTCTCTGT
GTTGCAGGCC
GCGAATATTC
TTTTGCCGAG
CAACACTCAT
AAAGACCGTT
ACAAGTTCAT
ACACTTGAAC
GTCCAGGAGT
TTTGTACAGC
CATTGCATTT
CTGATGGCAG
TACCCAACTA
TCTGATCCCC
TCAGGCAGCA
GAGAGTATAA
AGAGAAGAGA
GAACAATACT
CTGACTTTAA
TCAAGTGTTT
ACTTTCATTT
TTGGTCTTCT
AAATGCAAAC
AGGAGAAAGA
TTCTTGAGAC
ATCCTTTGGA
TACCAGCTAC
CGATGGTAGA
CAGCTTCAAG
TGGTACTCGG
TGGGATACAT
GAAACATTTG
GACCGTGACC
CGGAAAAGTT
GACGCACCAT
ATGCCTTTGT
TTTACTGTCT
CTATGAGAAT
CGGGAAGAAG
AATTTGTGAA
GACGATTGTG
GATGCTCTCA
TGGAGGTTAC
AGTTTACCTT
CAATCAGACA
AGGATATGAT
GGTCTCATTA
TACTGTCTGG
ATTACTGCTG
TCACCTGAGG
ACACTTAAGT
TGAGTGTTCA
GCGCATCTTT
CAAAACAAAG
AGCCACTTAT
AAGGCCAACT
GCTAGTCAAA
TGAAGAGCCT
TGTCTACTGG
AGAGAGATCT
GCTCTCTTTT
TTATACAATG
GAGAGCCTCC
GGGAGCTGCA
TATCTTTGAC
AATGACCTTA
ATGGTATTTC
AGAAAGGATT
CTGTCTAAAG
CCCTGGAGCA
TTCTAGCTGT
AAACTTCGCA
CACTCAAGTT
GTCCTATGTC
TCGACTGCTT
CTGGTTTTGA
AGACTTACTC
AAGGCTTTGG
CTCGTAATCG
GAGCCTGACA
TACCTGAGTA
TCAACTGTAC
GTCCATTTCC
CTAAATATGT
TTTCACTTCT
GCATGACATC
CTGCACGAGC
CCACATGCCA
AATAAGTCAT
CTGAGCTTGA
TGAAATGTGA
TTTGCGAGCC
AGCGAATGTG
AAGAAATCGC
CTCTCTCCTC
ATCAGTGGCG
GGAGTCTGAG
AAAACTGACC
TTATCCAGCA
ATCCGCTGAG
GAGCGACGGG
ATGAACATAC
TGTGTGATGC
CTTGCTTCAT
CCCAACTGCA
CTCTTATATC
ACTGGTGTTA
GTCTTCTGCT
GTCTCACTGA
AAATTGCTGC
AGCGCCCTTG
GAAGAGTGCT
TCTGTTCAGC
CCAACTCTAA
GACAACTAGA
CCTGTGTGTG
AATCGCTTAA
AAAATTACGG
AGTGTTGCAT
GTGACGTTTC
CCTTGCTGTT
TCCAACCTGT
CAGTTAGAGG
AGCTTCATCT
GTCTGGCTGT
TTCTTTAGCA
GCGATATCTG
TCAATATATT
GCCATAGTTA
TTGCTACTAA
TGAAAAACTG
AGGAGCCTGG
AGATTGGGAG
CAACAAAATA
GAAGATGCAG
GAATGCAGCT
GCTATGTGGT
GGTTTGAGAC
ATCCCAACTG
CATGTTGGTG
AATATTGGGC
TAGAAGAGTG
CATGTTAACC
AGTGCCTGCT
GTCGATCTCT
TGCCTCTGTT
CTTACCACCA
ACAAAACACT
AGAAAGACTC
AACTTGCTTC
AAAATCACTT
GGGCAATGAT
GGAGTTGCAA
AACTTCTTGA
GAGCTTGATC
AGCCCAGATT
GTGTACTTAA
GGTAGTTGGG
CTTCCATTAA
CTGGCCTGAA
CACACAAACC
CAGCAGTTGC
TGATGACTGT
AAAGGAAAGA
AAACCCAGTT
TGATCTTTCT
GTCTGAAACT
TGGTCTTTAA
AGGAAGGCAG
AGAAATTGGT
GTGACACCTG
CTTCTCAGCC
AGGTTCAATA
ATACCTAATT
CTAATTTGGA
TTACATGTTT
TTCAAATTTC
CCAGAATGTC
TGCAGCCATG
AGAACGTCAA
ATACAGCATC
TAGGCAACCC
CTTTGATCTA
GAACTATAGT
GAGTCGTATT
ACGTAGATCC
AGACATGATA
AGATACATTG
ATGAGTTTGG
ACAAACCACA
ACTAGAATGC
AGTGAAAAAA
ATGCTTTATT
TGTGAAATTT
GTGATGCTAT
TGCTTTATTT
GTAACCATTA
TAAGCTGCAA
TAAACAAGTT
AACAACAACA
ATTGCATTCA
TTTTATGTTT
CAGGTTCAGG
GGGAGGTGTG
GGAGGTTTTT
TAATTCGCGG
CCGCGGCGCC
AATGCATTGG
GCCCGGTACC
CAGCTTTTGT
TCCCTTTAGT
GAGGGTTAAT
TGCGCGCTTG
GCGTAATCAT
GGTCATAGCT
GTTTCCTGTG
TGAAATTGTT
ATCCGCTCAC
AATTCCACAC
AACATACGAG
CCGGAAGCAT
AAAGTGTAAA
GCCTGGGGTG
CCTAATGAGT
GAGCTAACTC
ACATTAATTG
CGTTGCGCTC
ACTGCCCGCT
TTCCAGTCGG
GAAACCTGTC
GTGCCAGCTG
CATTAATGAA
TCGGCCAACG
CGCGGGGAGA
GGCGGTTTGC
GTATTGGGCG
CTCTTCCGCT
TCCTCGCTCA
CTGACTCGCT
GCGCTCGGTC
GTTCGGCTGC
GGCGAGCGGT
ATCAGCTCAC
TCAAAGGCGG
TAATACGGTT
ATCCACAGAA
TCAGGGGATA
ACGCAGGAAA
GAACATGTGA
GCAAAAGGCC
AGCAAAAGGC
CAGGAACCGT
AAAAAGGCCG
CGTTGCTGGC
GTTTTTCCAT
AGGCTCCGCC
CCCCTGACGA
GCATCACAAA
AATCGACGCT
CAAGTCAGAG
GTGGCGAAAC
CCGACAGGAC
TATAAAGATA
CCAGGCGTTT
CCCCCTGGAA
GCTCCCTCGT
GCGCTCTCCT
GTTCCGACCC
TGCCGCTTAC
CGGATACCTG
TCCGCCTTTC
TCCCTTCGGG
AAGCGTGGCG
CTTTCTCATA
GCTCACGCTG
TAGGTATCTC
AGTTCGGTGT
AGGTCGTTCG
CTCCAAGCTG
GGCTGTGTGC
ACGAACCCCC
CGTTCAGCCC
GACCGCTGCG
CCTTATCCGG
TAACTATCGT
CTTGAGTCCA
ACCCGGTAAG
ACACGACTTA
TCGCCACTGG
CAGCAGCCAC
TGGTAACAGG
ATTAGCAGAG
CGAGGTATGT
AGGCGGTGCT
ACAGAGTTCT
TGAAGTGGTG
GCCTAACTAC
GGCTACACTA
GAAGGACAGT
ATTTGGTATC
TGCGCTCTGC
TGAAGCCAGT
TACCTTCGGA
AAAAGAGTTG
GTAGCTCTTG
ATCCGGCAAA
CAAACCACCG
CTGGTAGCGG
TGGTTTTTTT
GTTTGCAAGC
AGCAGATTAC
GCGCAGAAAA
AAAGGATCTC
AAGAAGATCC
TTTGATCTTT
TCTACGGGGT
CTGACGCTCA
GTGGAACGAA
AACTCACGTT
AAGGGATTTT
GGTCATGAGA
TTATCAAAAA
GGATCTTCAC
CTAGATCCTT
TTAAATTAAA
AATGAAGTTT
TAAATCAATC
TAAAGTATAT
ATGAGTAAAC
TTGGTCTGAC
AGTTACCAAT
GCTTAATCAG
TGAGGCACCT
ATCTCAGCGA
TCTGTCTATT
TCGTTCATCC
ATAGTTGCCT
GACTCCCCGT
CGTGTAGATA
ACTACGATAC
GGGAGGGCTT
ACCATCTGGC
CCCAGTGCTG
CAATGATACC
GCGAGACCCA
CGCTCACCGG
CTCCAGATTT
ATCAGCAATA
AACCAGCCAG
CCGGAAGGGC
CGAGCGCAGA
AGTGGTCCTG
CAACTTTATC
CGCCTCCATC
CAGTCTATTA
ATTGTTGCCG
GGAAGCTAGA
GTAAGTAGTT
CGCCAGTTAA
TAGTTTGCGC
AACGTTGTTG
CCATTGCTAC
AGGCATCGTG
GTGTCACGCT
CGTCGTTTGG
TATGGCTTCA
TTCAGCTCCG
GTTCCCAACG
ATCAAGGCGA
GTTACATGAT
CCCCCATGTT
GTGCAAAAAA
GCGGTTAGCT
CCTTCGGTCC
TCCGATCGTT
GTCAGAAGTA
AGTTGGCCGC
AGTGTTATCA
CTCATGGTTA
TGGCAGCACT
GCATAATTCT
CTTACTGTCA
TGCCATCCGT
AAGATGCTTT
TCTGTGACTG
GTGAGTACTC
AACCAAGTCA
TTCTGAGAAT
AGTGTATGCG
GCGACCGAGT
TGCTCTTGCC
CGGCGTCAAT
ACGGGATAAT
ACCGCGCCAC
ATAGCAGAAC
TTTAAAAGTG
CTCATCATTG
GAAAACGTTC
TTCGGGGCGA
AAACTCTCAA
GGATCTTACC
GCTGTTGAGA
TCCAGTTCGA
TGTAACCCAC
TCGTGCACCC
AACTGATCTT
CAGCATCTTT
TACTTTCACC
AGCGTTTCTG
GGTGAGCAAA
AACAGGAAGG
CAAAATGCCG
CAAAAAAGGG
AATAAGGGCG
ACACGGAAAT
GTTGAATACT
CATACTCTTC
CTTTTTCAAT
ATTATTGAAG
CATTTATCAG
GGTTATTGTC
TCATGAGCGG
ATACATATTT
GAATGTATTT
AGAAAAATAA
ACAAATAGGG
GTTCCGCGCA
CATTTCCCCG
AAAAGTGCCA
CCTAAATTGT
AAGCGTTAAT
ATTTTGTTAA
AATTCGCGTT
AAATTTTTGT
TAAATCAGCT
CATTTTTTAA
CCAATAGGCC
GAAATCGGCA
AAATCCCTTA
TAAATCAAAA
GAATAGACCG
AGATAGGGTT
GAGTGTTGTT
CCAGTTTGGA
ACAAGAGTCC
ACTATTAAAG
AACGTGGACT
CCAACGTCAA
AGGGCGAAAA
ACCGTCTATC
AGGGCGATGG
CCCACTACGT
GAACCATCAC
CCTAATCAAG
TTTTTTGGGG
TCGAGGTGCC
GTAAAGCACT
AAATCGGAAC
CCTAAAGGGA
GCCCCCGATT
TAGAGCTTGA
CGGGGAAAGC
CGGCGAACGT
GGCGAGAAAG
GAAGGGAAGA
AAGCGAAAGG
AGCGGGCGCT
AGGGCGCTGG
CAAGTGTAGC
GGTCACGCTG
CGCGTAACCA
CCACACCCGC
CGCGCTTAAT
GCGCCGCTAC
AGGGCGCGTC
CCATTCGCCA
TTCAGGCTGC
GCAACTGTTG
GGAAGGGCGA
TCGGTGCGGG
CCTCTTCGCT
ATTACGCCAG
TCGACCATAG
CCAATTCAAT
ATGGCGTATA
TGGACTCATG
CCAATTCAAT
ATGGTGGATC
TGGACCTGTG
CCAATTCAAT
ATGGCGTATA
TGGACTCGTG
CCAATTCAAT
ATGGTGGATC
TGGACCCCAG
CCAATTCAAT
ATGGCGGACT
TGGCACCATG
CCAATTCAAT
ATGGCGGACT
TGGCACTGTG
CCAACTGGGG
AGGGGTCTAC
TTGGCACGGT
GCCAAGTTTG
AGGAGGGGTC
TTGGCCCTGT
GCCAAGTCCG
CCATATTGAA
TTGGCATGGT
GCCAATAATG
GCGGCCATAT
TGGCTATATG
CCAGGATCAA
TATATAGGCA
ATATCCAATA
TGGCCCTATG
CCAATATGGC
TATTGGCCAG
GTTCAATACT
ATGTATTGGC
CCTATGCCAT
ATAGTATTCC
ATATATGGGT
TTTCCTATTG
ACGTAGATAG
CCCCTCCCAA
TGGGCGGTCC
CATATACCAT
ATATGGGGCT
TCCTAATACC
GCCCATAGCC
ACTCCCCCAT
TGACGTCAAT
GGTCTCTATA
TATGGTCTTT
CCTATTGACG
TCATATGGGC
GGTCCTATTG
ACGTATATGG
CGCCTCCCCC
ATTGACGTCA
ATTACGGTAA
ATGGCCCGCC
TGGCTCAATG
CCCATTGACG
TCAATAGGAC
CACCCACCAT
TGACGTCAAT
GGGATGGCTC
ATTGCCCATT
CATATCCGTT
CTCACGCCCC
CTATTGACGT
CAATGACGGT
AAATGGCCCA
CTTGGCAGTA
CATCAATATC
TATTAATAGT
AACTTGGCAA
GTACATTACT
ATTGGAAGGA
CGCCAGGGTA
CATTGGCAGT
ACTCCCATTG
ACGTCAATGG
CGGTAAATGG
CCCGCGATGG
CTGCCAAGTA
CATCCCCATT
GACGTCAATG
GGGAGGGGCA
ATGACGCAAA
TGGGCGTTCC
ATTGACGTAA
ATGGGCGGTA
GGCGTGCCTA
ATGGGAGGTC
TATATAAGCA
ATGCTCGTTT
AGGGAAC