收藏网址 | 中文

pCS2-6×Myc-NLRP11(human)

产品货号:P52066

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pCS2-6×Myc-NLRP11(human)
别称: NM_145007.5;CLR19.6; NALP11; NOD17; PAN10; PYPAF6;PYPAF7
基因长: 3099bp
质粒宿主: 哺乳细胞,鱼类
质粒用途: 蛋白表达
片段类型: ORF
片段物种:
原核抗性: Amp
筛选标记:
荧光标记:
启动子: CMV
复制子: pUC
拷贝数:
感受态: DH5a
温度: 37度
背景载体:
正向引物: CMV-F
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

CGCCATTCTG

CCTGGGGACG

TCGGAGCAAG

CTTGATTTAG

GTGACACTAT

AGAATACAAG

61

CTACTTGTTC

TTTTTGCAGG

ATCCCATCGA

TTTAAAGCTA

TGGAGCAAAA

GCTCATTTCT

121

GAAGAGGACT

TGAATGAAAT

GGAGCAAAAG

CTCATTTCTG

AAGAGGACTT

GAATGAAATG

181

GAGCAAAAGC

TCATTTCTGA

AGAGGACTTG

AATGAAATGG

AGCAAAAGCT

CATTTCTGAA

241

GAGGACTTGA

ATGAAATGGA

GCAAAAGCTC

ATTTCTGAAG

AGGACTTGAA

TGAAATGGAG

301

AGCTTGGGCG

ACCTCACCAT

GGAGCAAAAG

CTCATTTCTG

AAGAGGACTT

GAATTCAGCA

361

GAATCGGATT

CTACTGACTT

TGACCTGCTG

TGGTATCTAG

AGAATCTCAG

TGACAAGGAA

421

TTTCAGAGTT

TTAAGAAGTA

TCTGGCACGC

AAGATTCTTG

ATTTCAAACT

GCCACAGTTT

481

CCACTGATAC

AGATGACAAA

AGAAGAACTG

GCTAACGTGT

TGCCAATCTC

TTATGAGGGA

541

CAGTATATAT

GGAATATGCT

CTTCAGCATA

TTTTCAATGA

TGCGTAAGGA

AGATCTTTGT

601

AGGAAGATCA

TTGGCAGACG

AAACCGCAAT

CAGGAGGCAT

GCAAAGCTGT

CATGAGGAGA

661

AAATTCATGC

TGCAATGGGA

AAGTCACACT

TTTGGAAAAT

TTCATTATAA

ATTTTTTCGT

721

GACGTTTCGT

CAGATGTGTT

CTACATACTT

CAATTAGCCT

ATGATTCTAC

CAGCTATTAT

781

TCAGCAAACA

ATCTCAATGT

GTTCCTGATG

GGAGAGAGAG

CATCTGGAAA

AACTATTGTT

841

ATAAATCTGG

CTGTGTTGAG

GTGGATCAAG

GGTGAGATGT

GGCAGAACAT

GATCTCGTAC

901

GTCGTTCACC

TCACTGCTCA

CGAAATAAAC

CAGATGACCA

ACAGCAGCTT

GGCTGAGCTA

961

ATCGCCAAGG

ACTGGCCTGA

CGGCCAGGCT

CCCATTGCAG

ACATCCTGTC

TGATCCCAAG

1021

AAACTCCTTT

TCATCCTCGA

GGACTTGGAC

AACATAAGAT

TCGAGTTAAA

TGTCAATGAA

1081

AGTGCTTTGT

GTAGTAACAG

CACCCAGAAA

GTTCCCATTC

CAGTTCTCCT

GGTCAGTTTG

1141

CTGAAGAGAA

AAATGGCTCC

AGGCTGCTGG

TTCCTCATCT

CCTCAAGGCC

CACACGTGGG

1201

AATAATGTAA

AAACGTTCTT

GAAAGAGGTA

GATTGCTGCA

CGACCTTGCA

GCTGTCGAAT

1261

GGGAAGAGGG

AGATATATTT

TAACTCTTTC

TTTAAAGACC

GCCAGAGGGC

GTCGGCAGCC

1321

CTCCAGCTTG

TACATGAGGA

TGAAATACTC

GTGGGTCTGT

GCCGAGTCGC

CATCTTATGC

1381

TGGATCACGT

GTACTGTCCT

GAAGCGGCAG

ATGGACAAGG

GGCGTGACTT

CCAGCTCTGC

1441

TGCCAAACAC

CCACTGATCT

ACATGCCCAC

TTTCTTGCTG

ATGCGTTGAC

ATCAGAGGCT

1501

GGACTTACTG

CCAATCAGTA

TCACCTAGGT

CTCCTAAAAC

GTCTGTGTTT

GCTGGCTGCA

1561

GGAGGACTGT

TTCTGAGCAC

CCTGAATTTC

AGTGGTGAAG

ACCTCAGATG

TGTTGGGTTT

1621

ACTGAGGCTG

ATGTCTCTGT

GTTGCAGGCC

GCGAATATTC

TTTTGCCGAG

CAACACTCAT

1681

AAAGACCGTT

ACAAGTTCAT

ACACTTGAAC

GTCCAGGAGT

TTTGTACAGC

CATTGCATTT

1741

CTGATGGCAG

TACCCAACTA

TCTGATCCCC

TCAGGCAGCA

GAGAGTATAA

AGAGAAGAGA

1801

GAACAATACT

CTGACTTTAA

TCAAGTGTTT

ACTTTCATTT

TTGGTCTTCT

AAATGCAAAC

1861

AGGAGAAAGA

TTCTTGAGAC

ATCCTTTGGA

TACCAGCTAC

CGATGGTAGA

CAGCTTCAAG

1921

TGGTACTCGG

TGGGATACAT

GAAACATTTG

GACCGTGACC

CGGAAAAGTT

GACGCACCAT

1981

ATGCCTTTGT

TTTACTGTCT

CTATGAGAAT

CGGGAAGAAG

AATTTGTGAA

GACGATTGTG

2041

GATGCTCTCA

TGGAGGTTAC

AGTTTACCTT

CAATCAGACA

AGGATATGAT

GGTCTCATTA

2101

TACTGTCTGG

ATTACTGCTG

TCACCTGAGG

ACACTTAAGT

TGAGTGTTCA

GCGCATCTTT

2161

CAAAACAAAG

AGCCACTTAT

AAGGCCAACT

GCTAGTCAAA

TGAAGAGCCT

TGTCTACTGG

2221

AGAGAGATCT

GCTCTCTTTT

TTATACAATG

GAGAGCCTCC

GGGAGCTGCA

TATCTTTGAC

2281

AATGACCTTA

ATGGTATTTC

AGAAAGGATT

CTGTCTAAAG

CCCTGGAGCA

TTCTAGCTGT

2341

AAACTTCGCA

CACTCAAGTT

GTCCTATGTC

TCGACTGCTT

CTGGTTTTGA

AGACTTACTC

2401

AAGGCTTTGG

CTCGTAATCG

GAGCCTGACA

TACCTGAGTA

TCAACTGTAC

GTCCATTTCC

2461

CTAAATATGT

TTTCACTTCT

GCATGACATC

CTGCACGAGC

CCACATGCCA

AATAAGTCAT

2521

CTGAGCTTGA

TGAAATGTGA

TTTGCGAGCC

AGCGAATGTG

AAGAAATCGC

CTCTCTCCTC

2581

ATCAGTGGCG

GGAGTCTGAG

AAAACTGACC

TTATCCAGCA

ATCCGCTGAG

GAGCGACGGG

2641

ATGAACATAC

TGTGTGATGC

CTTGCTTCAT

CCCAACTGCA

CTCTTATATC

ACTGGTGTTA

2701

GTCTTCTGCT

GTCTCACTGA

AAATTGCTGC

AGCGCCCTTG

GAAGAGTGCT

TCTGTTCAGC

2761

CCAACTCTAA

GACAACTAGA

CCTGTGTGTG

AATCGCTTAA

AAAATTACGG

AGTGTTGCAT

2821

GTGACGTTTC

CCTTGCTGTT

TCCAACCTGT

CAGTTAGAGG

AGCTTCATCT

GTCTGGCTGT

2881

TTCTTTAGCA

GCGATATCTG

TCAATATATT

GCCATAGTTA

TTGCTACTAA

TGAAAAACTG

2941

AGGAGCCTGG

AGATTGGGAG

CAACAAAATA

GAAGATGCAG

GAATGCAGCT

GCTATGTGGT

3001

GGTTTGAGAC

ATCCCAACTG

CATGTTGGTG

AATATTGGGC

TAGAAGAGTG

CATGTTAACC

3061

AGTGCCTGCT

GTCGATCTCT

TGCCTCTGTT

CTTACCACCA

ACAAAACACT

AGAAAGACTC

3121

AACTTGCTTC

AAAATCACTT

GGGCAATGAT

GGAGTTGCAA

AACTTCTTGA

GAGCTTGATC

3181

AGCCCAGATT

GTGTACTTAA

GGTAGTTGGG

CTTCCATTAA

CTGGCCTGAA

CACACAAACC

3241

CAGCAGTTGC

TGATGACTGT

AAAGGAAAGA

AAACCCAGTT

TGATCTTTCT

GTCTGAAACT

3301

TGGTCTTTAA

AGGAAGGCAG

AGAAATTGGT

GTGACACCTG

CTTCTCAGCC

AGGTTCAATA

3361

ATACCTAATT

CTAATTTGGA

TTACATGTTT

TTCAAATTTC

CCAGAATGTC

TGCAGCCATG

3421

AGAACGTCAA

ATACAGCATC

TAGGCAACCC

CTTTGATCTA

GAACTATAGT

GAGTCGTATT

3481

ACGTAGATCC

AGACATGATA

AGATACATTG

ATGAGTTTGG

ACAAACCACA

ACTAGAATGC

3541

AGTGAAAAAA

ATGCTTTATT

TGTGAAATTT

GTGATGCTAT

TGCTTTATTT

GTAACCATTA

3601

TAAGCTGCAA

TAAACAAGTT

AACAACAACA

ATTGCATTCA

TTTTATGTTT

CAGGTTCAGG

3661

GGGAGGTGTG

GGAGGTTTTT

TAATTCGCGG

CCGCGGCGCC

AATGCATTGG

GCCCGGTACC

3721

CAGCTTTTGT

TCCCTTTAGT

GAGGGTTAAT

TGCGCGCTTG

GCGTAATCAT

GGTCATAGCT

3781

GTTTCCTGTG

TGAAATTGTT

ATCCGCTCAC

AATTCCACAC

AACATACGAG

CCGGAAGCAT

3841

AAAGTGTAAA

GCCTGGGGTG

CCTAATGAGT

GAGCTAACTC

ACATTAATTG

CGTTGCGCTC

3901

ACTGCCCGCT

TTCCAGTCGG

GAAACCTGTC

GTGCCAGCTG

CATTAATGAA

TCGGCCAACG

3961

CGCGGGGAGA

GGCGGTTTGC

GTATTGGGCG

CTCTTCCGCT

TCCTCGCTCA

CTGACTCGCT

4021

GCGCTCGGTC

GTTCGGCTGC

GGCGAGCGGT

ATCAGCTCAC

TCAAAGGCGG

TAATACGGTT

4081

ATCCACAGAA

TCAGGGGATA

ACGCAGGAAA

GAACATGTGA

GCAAAAGGCC

AGCAAAAGGC

4141

CAGGAACCGT

AAAAAGGCCG

CGTTGCTGGC

GTTTTTCCAT

AGGCTCCGCC

CCCCTGACGA

4201

GCATCACAAA

AATCGACGCT

CAAGTCAGAG

GTGGCGAAAC

CCGACAGGAC

TATAAAGATA

4261

CCAGGCGTTT

CCCCCTGGAA

GCTCCCTCGT

GCGCTCTCCT

GTTCCGACCC

TGCCGCTTAC

4321

CGGATACCTG

TCCGCCTTTC

TCCCTTCGGG

AAGCGTGGCG

CTTTCTCATA

GCTCACGCTG

4381

TAGGTATCTC

AGTTCGGTGT

AGGTCGTTCG

CTCCAAGCTG

GGCTGTGTGC

ACGAACCCCC

4441

CGTTCAGCCC

GACCGCTGCG

CCTTATCCGG

TAACTATCGT

CTTGAGTCCA

ACCCGGTAAG

4501

ACACGACTTA

TCGCCACTGG

CAGCAGCCAC

TGGTAACAGG

ATTAGCAGAG

CGAGGTATGT

4561

AGGCGGTGCT

ACAGAGTTCT

TGAAGTGGTG

GCCTAACTAC

GGCTACACTA

GAAGGACAGT

4621

ATTTGGTATC

TGCGCTCTGC

TGAAGCCAGT

TACCTTCGGA

AAAAGAGTTG

GTAGCTCTTG

4681

ATCCGGCAAA

CAAACCACCG

CTGGTAGCGG

TGGTTTTTTT

GTTTGCAAGC

AGCAGATTAC

4741

GCGCAGAAAA

AAAGGATCTC

AAGAAGATCC

TTTGATCTTT

TCTACGGGGT

CTGACGCTCA

4801

GTGGAACGAA

AACTCACGTT

AAGGGATTTT

GGTCATGAGA

TTATCAAAAA

GGATCTTCAC

4861

CTAGATCCTT

TTAAATTAAA

AATGAAGTTT

TAAATCAATC

TAAAGTATAT

ATGAGTAAAC

4921

TTGGTCTGAC

AGTTACCAAT

GCTTAATCAG

TGAGGCACCT

ATCTCAGCGA

TCTGTCTATT

4981

TCGTTCATCC

ATAGTTGCCT

GACTCCCCGT

CGTGTAGATA

ACTACGATAC

GGGAGGGCTT

5041

ACCATCTGGC

CCCAGTGCTG

CAATGATACC

GCGAGACCCA

CGCTCACCGG

CTCCAGATTT

5101

ATCAGCAATA

AACCAGCCAG

CCGGAAGGGC

CGAGCGCAGA

AGTGGTCCTG

CAACTTTATC

5161

CGCCTCCATC

CAGTCTATTA

ATTGTTGCCG

GGAAGCTAGA

GTAAGTAGTT

CGCCAGTTAA

5221

TAGTTTGCGC

AACGTTGTTG

CCATTGCTAC

AGGCATCGTG

GTGTCACGCT

CGTCGTTTGG

5281

TATGGCTTCA

TTCAGCTCCG

GTTCCCAACG

ATCAAGGCGA

GTTACATGAT

CCCCCATGTT

5341

GTGCAAAAAA

GCGGTTAGCT

CCTTCGGTCC

TCCGATCGTT

GTCAGAAGTA

AGTTGGCCGC

5401

AGTGTTATCA

CTCATGGTTA

TGGCAGCACT

GCATAATTCT

CTTACTGTCA

TGCCATCCGT

5461

AAGATGCTTT

TCTGTGACTG

GTGAGTACTC

AACCAAGTCA

TTCTGAGAAT

AGTGTATGCG

5521

GCGACCGAGT

TGCTCTTGCC

CGGCGTCAAT

ACGGGATAAT

ACCGCGCCAC

ATAGCAGAAC

5581

TTTAAAAGTG

CTCATCATTG

GAAAACGTTC

TTCGGGGCGA

AAACTCTCAA

GGATCTTACC

5641

GCTGTTGAGA

TCCAGTTCGA

TGTAACCCAC

TCGTGCACCC

AACTGATCTT

CAGCATCTTT

5701

TACTTTCACC

AGCGTTTCTG

GGTGAGCAAA

AACAGGAAGG

CAAAATGCCG

CAAAAAAGGG

5761

AATAAGGGCG

ACACGGAAAT

GTTGAATACT

CATACTCTTC

CTTTTTCAAT

ATTATTGAAG

5821

CATTTATCAG

GGTTATTGTC

TCATGAGCGG

ATACATATTT

GAATGTATTT

AGAAAAATAA

5881

ACAAATAGGG

GTTCCGCGCA

CATTTCCCCG

AAAAGTGCCA

CCTAAATTGT

AAGCGTTAAT

5941

ATTTTGTTAA

AATTCGCGTT

AAATTTTTGT

TAAATCAGCT

CATTTTTTAA

CCAATAGGCC

6001

GAAATCGGCA

AAATCCCTTA

TAAATCAAAA

GAATAGACCG

AGATAGGGTT

GAGTGTTGTT

6061

CCAGTTTGGA

ACAAGAGTCC

ACTATTAAAG

AACGTGGACT

CCAACGTCAA

AGGGCGAAAA

6121

ACCGTCTATC

AGGGCGATGG

CCCACTACGT

GAACCATCAC

CCTAATCAAG

TTTTTTGGGG

6181

TCGAGGTGCC

GTAAAGCACT

AAATCGGAAC

CCTAAAGGGA

GCCCCCGATT

TAGAGCTTGA

6241

CGGGGAAAGC

CGGCGAACGT

GGCGAGAAAG

GAAGGGAAGA

AAGCGAAAGG

AGCGGGCGCT

6301

AGGGCGCTGG

CAAGTGTAGC

GGTCACGCTG

CGCGTAACCA

CCACACCCGC

CGCGCTTAAT

6361

GCGCCGCTAC

AGGGCGCGTC

CCATTCGCCA

TTCAGGCTGC

GCAACTGTTG

GGAAGGGCGA

6421

TCGGTGCGGG

CCTCTTCGCT

ATTACGCCAG

TCGACCATAG

CCAATTCAAT

ATGGCGTATA

6481

TGGACTCATG

CCAATTCAAT

ATGGTGGATC

TGGACCTGTG

CCAATTCAAT

ATGGCGTATA

6541

TGGACTCGTG

CCAATTCAAT

ATGGTGGATC

TGGACCCCAG

CCAATTCAAT

ATGGCGGACT

6601

TGGCACCATG

CCAATTCAAT

ATGGCGGACT

TGGCACTGTG

CCAACTGGGG

AGGGGTCTAC

6661

TTGGCACGGT

GCCAAGTTTG

AGGAGGGGTC

TTGGCCCTGT

GCCAAGTCCG

CCATATTGAA

6721

TTGGCATGGT

GCCAATAATG

GCGGCCATAT

TGGCTATATG

CCAGGATCAA

TATATAGGCA

6781

ATATCCAATA

TGGCCCTATG

CCAATATGGC

TATTGGCCAG

GTTCAATACT

ATGTATTGGC

6841

CCTATGCCAT

ATAGTATTCC

ATATATGGGT

TTTCCTATTG

ACGTAGATAG

CCCCTCCCAA

6901

TGGGCGGTCC

CATATACCAT

ATATGGGGCT

TCCTAATACC

GCCCATAGCC

ACTCCCCCAT

6961

TGACGTCAAT

GGTCTCTATA

TATGGTCTTT

CCTATTGACG

TCATATGGGC

GGTCCTATTG

7021

ACGTATATGG

CGCCTCCCCC

ATTGACGTCA

ATTACGGTAA

ATGGCCCGCC

TGGCTCAATG

7081

CCCATTGACG

TCAATAGGAC

CACCCACCAT

TGACGTCAAT

GGGATGGCTC

ATTGCCCATT

7141

CATATCCGTT

CTCACGCCCC

CTATTGACGT

CAATGACGGT

AAATGGCCCA

CTTGGCAGTA

7201

CATCAATATC

TATTAATAGT

AACTTGGCAA

GTACATTACT

ATTGGAAGGA

CGCCAGGGTA

7261

CATTGGCAGT

ACTCCCATTG

ACGTCAATGG

CGGTAAATGG

CCCGCGATGG

CTGCCAAGTA

7321

CATCCCCATT

GACGTCAATG

GGGAGGGGCA

ATGACGCAAA

TGGGCGTTCC

ATTGACGTAA

7381

ATGGGCGGTA

GGCGTGCCTA

ATGGGAGGTC

TATATAAGCA

ATGCTCGTTT

AGGGAAC

武汉网监电子标识 Copyright © 武汉淼灵生物科技有限公司版权所有/鄂公网安备42010702000282号 鄂ICP备14012402号-1
访客数:2972992
1.325211s