名称: | pBS-CMV-Gag-Pro-CRE |
---|---|
别称: | |
基因长: | 3180bp |
质粒宿主: | 哺乳细胞 |
质粒用途: | 蛋白表达 |
片段类型: | ORF |
片段物种: | 病毒 |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | CMV |
复制子: | pUC |
拷贝数: | |
感受态: | DH5a |
温度: | 37度 |
背景载体: | |
正向引物: | CMV-F |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ATAAATCAAT
ATTGGCTATT
GGCCATTGCA
TACGTTGTAT
CCATATCATA
ATATGTACAT
TTATATTGGC
TCATGTCCAA
CATTACCGCC
ATGTTGACAT
TGATTATTGA
CTAGTTATTA
ATAGTAATCA
ATTACGGGGT
CATTAGTTCA
TAGCCCATAT
ATGGAGTTCC
GCGTTACATA
ACTTACGGTA
AATGGCCCGC
CTGGCTGACC
GCCCAACGAC
CCCCGCCCAT
TGACGTCAAT
AATGACGTAT
GTTCCCATAG
TAACGCCAAT
AGGGACTTTC
CATTGACGTC
AATGGGTGGA
GTATTTACGG
TAAACTGCCC
ACTTGGCAGT
ACATCAAGTG
TATCATATGC
CAAGTACGCC
CCCTATTGAC
GTCAATGACG
GTAAATGGCC
CGCCTGGCAT
TATGCCCAGT
ACATGACCTT
ATGGGACTTT
CCTACTTGGC
AGTACATCTA
CGTATTAGTC
ATCGCTATTA
CCATGGTGAT
GCGGTTTTGG
CAGTACATCA
ATGGGCGTGG
ATAGCGGTTT
GACTCACGGG
GATTTCCAAG
TCTCCACCCC
ATTGACGTCA
ATGGGAGTTT
GTTTTGGCAC
CAAAATCAAC
GGGACTTTCC
AAAATGTCGT
AACAACTCCG
CCCCATTGAC
GCAAATGGGC
GGTAGGCGTG
TACGGTGGGA
GGTCTATATA
AGCAGAGCTC
GTTTAGTGAA
CCGTCAGATC
GCCTGGAGAC
GCCATCCACG
CTGTTTTGAC
CTCCATAGAA
GACACCGGGA
CCGATCCAGC
CTCCGCGGCC
CCAAGCTTGG
GCATCGTTCT
GTGTTGTCTC
TGTCTGACTG
TGTTTCTGTA
TTTGTCTGAA
AATATGGGCC
AGACTGTTAC
CACTCCCTTA
AGTTTGACCT
TAGGTCACTG
GAAAGATGTC
GAGCGGATCG
CTCACAACCA
GTCGGTAGAT
GTCAAGAAGA
GACGTTGGGT
TACCTTCTGC
TCTGCAGAAT
GGCCAACCTT
TAACGTCGGA
TGGCCGCGAG
ACGGCACCTT
TAACCGAGAC
CTCATCACCC
AGGTTAAGAT
CAAGGTCTTT
TCACCTGGCC
CGCATGGACA
CCCAGACCAG
GTCCCCTACA
TCGTGACCTG
GGAAGCCTTG
GCTTTTGACC
CCCCTCCCTG
GGTCAAGCCC
TTTGTACACC
CTAAGCCTCC
GCCTCCTCTT
CCTCCATCCG
CCCCGTCTCT
CCCCCTTGAA
CCTCCTCGTT
CGACCCCGCC
TCGATCCTCC
CTTTATCCAG
CCCTCACTCC
TTCTCTAGGC
GCCAAACCTA
AACCTCAAGT
TCTTTCTGAC
AGTGGGGGGC
CGCTCATCGA
CCTACTTACA
GAAGACCCCC
CGCCTTATAG
GGACCCAAGA
CCACCCCCTT
CCGACAGGGA
CGGAAATGGT
GGAGAAGCGA
CCCCTGCGGG
AGAGGCACCG
GACCCCTCCC
CAATGGCATC
TCGCCTACGT
GGGAGACGGG
AGCCCCCTGT
GGCCGACTCC
ACTACCTCGC
AGGCATTCCC
CCTCCGCGCA
GGAGGAAACG
GACAGCTTCA
ATACTGGCCG
TTCTCCTCTT
CTGACCTTTA
CAACTGGAAA
AATAATAACC
CTTCTTTTTC
TGAAGATCCA
GGTAAACTGA
CAGCTCTGAT
CGAGTCTGTC
CTCATCACCC
ATCAGCCCAC
CTGGGACGAC
TGTCAGCAGC
TGTTGGGGAC
TCTGCTGACC
GGAGAAGAAA
AACAACGGGT
GCTCTTAGAG
GCTAGAAAGG
CGGTGCGGGG
CGATGATGGG
CGCCCCACTC
AACTGCCCAA
TGAAGTCGAT
GCCGCTTTTC
CCCTCGAGCG
CCCAGACTGG
GATTACACCA
CCCAGGCAGG
TAGGAACCAC
CTAGTCCACT
ATCGCCAGTT
GCTCCTAGCG
GGTCTCCAAA
ACGCGGGCAG
AAGCCCCACC
AATTTGGCCA
AGGTAAAAGG
AATAACACAA
GGGCCCAATG
AGTCTCCCTC
GGCCTTCCTA
GAGAGACTTA
AGGAAGCCTA
TCGCAGGTAC
ACTCCTTATG
ACCCTGAGGA
CCCAGGGCAA
GAAACTAATG
TGTCTATGTC
TTTCATTTGG
CAGTCTGCCC
CAGACATTGG
GAGAAAGTTA
GAGAGGTTAG
AAGATTTAAA
AAACAAGACG
CTTGGAGATT
TGGTTAGAGA
GGCAGAAAAG
ATCTTTAATA
AACGAGAAAC
CCCGGAAGAA
AGAGAGGAAC
GTATCAGGAG
AGAAACAGAG
GAAAAAGAAG
AACGCCGTAG
GACAGAGGAT
GAGCAGAAAG
AGAAAGAAAG
AGATCGTAGG
AGACATAGAG
AGATGAGCAA
GCTATTGGCC
ACTGTCGTTA
GTGGACAGAA
ACAGGATAGA
CAGGGAGGAG
AACGAAGGAG
GTCCCAACTC
GATCGCGACC
AGTGTGCCTA
CTGCAAAGAA
AAGGGGCACT
GGGCTAAAGA
TTGTCCCAAG
AAACCACGAG
GACCTCGGGG
ACCAAGACCC
CAGACCTCCC
TCCTGACCCT
AGATGACCAG
GGAGGTCAGG
GTCAGGAGCC
CCCCCCTGAA
CCCAGGATAA
CCCTCAAAGT
CGGGGGGCAA
CCCGTCACCT
TCCTGGTAGA
TACTGGGGCC
CAACACTCCG
TGCTGACCCA
AAATCCTGGA
CCCCTAAGTG
ATAAGTCTGC
CTGGGTCCAA
GGGGCTACTG
GAGGAAAGCG
GTATCGCTGG
ACCACGGATC
GCAAAGTACA
TCTAGCTACC
GGTAAGGTCA
CCCACTCTTT
CCTCCATGTA
CCAGACTGTC
CCTATCCTCT
GTTAGGAAGA
GATTTGCTGA
CTAAACTAAA
AGCCCAAATC
CACTTTGAGG
GATCAGGAGC
TCAGGTTATG
GGACCAATGG
GGCAGCCCCT
GCAAGTGTTG
GGAAGCGGAG
AGGGCAGGGG
AAGTCTTCTA
ACATGCGGGG
ACGTGGAGGA
AAATCCCGGC
CCCACGTCCA
CGCTGCTAAT
GGAGAACTCG
TCTGGAGATT
ACAAAGACGA
TGACGATAAG
AAACGGACAG
CCGACGGAAG
CGAGTTCGAG
TCACCAAAGA
AGAAGCGGAA
AGTCTCGAAT
TTACTGACCG
TACACCAAAA
TTTGCCTGCA
TTACCGGTCG
ATGCAACGAG
TGATGAGGTT
CGCAAGAACC
TGATGGACAT
GTTCAGGGAT
CGCCAGGCGT
TTTCTGAGCA
TACCTGGAAA
ATGCTTCTGT
CCGTTTGCCG
GTCGTGGGCG
GCATGGTGCA
AGTTGAATAA
CCGGAAATGG
TTTCCCGCAG
AACCTGAAGA
TGTTCGCGAT
TATCTTCTAT
ATCTTCAGGC
GCGCGGTCTG
GCAGTAAAAA
CTATCCAGCA
ACATTTGGGC
CAGCTAAACA
TGCTTCATCG
TCGGTCCGGG
CTGCCACGAC
CAAGTGACAG
CAATGCTGTT
TCACTGGTTA
TGCGGCGGAT
CCGAAAAGAA
AACGTTGATG
CCGGTGAACG
TGCAAAACAG
GCTCTAGCGT
TCGAACGCAC
TGATTTCGAC
CAGGTTCGTT
CACTCATGGA
AAATAGCGAT
CGCTGCCAGG
ATATACGTAA
TCTGGCATTT
CTGGGGATTG
CTTATAACAC
CCTGTTACGT
ATAGCCGAAA
TTGCCAGGAT
CAGGGTTAAA
GATATCTCAC
GTACTGACGG
TGGGAGAATG
TTAATCCATA
TTGGCAGAAC
GAAAACGCTG
GTTAGCACCG
CAGGTGTAGA
GAAGGCACTT
AGCCTGGGGG
TAACTAAACT
GGTCGAGCGA
TGGATTTCCG
TCTCTGGTGT
AGCTGATGAT
CCGAATAACT
ACCTGTTTTG
CCGGGTCAGA
AAAAATGGTG
TTGCCGCGCC
ATCTGCCACC
AGCCAGCTAT
CAACTCGCGC
CCTGGAAGGG
ATTTTTGAAG
CAACTCATCG
ATTGATTTAC
GGCGCTAAGG
ATGACTCTGG
TCAGAGATAC
CTGGCCTGGT
CTGGACACAG
TGCCCGTGTC
GGAGCCGCGC
GAGATATGGC
CCGCGCTGGA
GTTTCAATAC
CGGAGATCAT
GCAAGCTGGT
GGCTGGACCA
ATGTAAATAT
TGTCATGAAC
TATATCCGTA
ACCTGGATAG
TGAAACAGGG
GCAATGGTGC
GCCTGCTGGA
AGATGGCGAT
TAAGAATTCC
TGCAGCCCCG
CTGATCAGCC
TCGACTGTGC
CTTCTAGTTG
CCAGCCATCT
GTTGTTTGCC
CCTCCCCCGT
GCCTTCCTTG
ACCCTGGAAG
GTGCCACTCC
CACTGTCCTT
TCCTAATAAA
ATGAGGAAAT
TGCATCGCAT
TGTCTGAGTA
GGTGTCATTC
TATTCTGGGG
GGTGGGGTGG
GGCAGGACAG
CAAGGGGGAG
GATTGGGAAG
ACAATAGCAG
GCATGCTGGG
GATGCGGTGG
GCTCTATGGC
TTCTGAGGCG
GAAAGAACCA
GCTGGGGCTC
GATCCTCTAG
AGCGGCCGCC
ACCGCGGTGG
AGCTCCAATT
CGCCCTATAG
TGAGTCGTAT
TACAATTCAC
TGGCCGTCGT
TTTACAACGT
CGTGACTGGG
AAAACCCTGG
CGTTACCCAA
CTTAATCGCC
TTGCAGCACA
TCCCCCTTTC
GCCAGCTGGC
GTAATAGCGA
AGAGGCCCGC
ACCGATCGCC
CTTCCCAACA
GTTGCGCAGC
CTGAATGGCG
AATGGGACGC
GCCCTGTAGC
GGCGCATTAA
GCGCGGCGGG
TGTGGTGGTT
ACGCGCAGCG
TGACCGCTAC
ACTTGCCAGC
GCCCTAGCGC
CCGCTCCTTT
CGCTTTCTTC
CCTTCCTTTC
TCGCCACGTT
CGCCGGCTTT
CCCCGTCAAG
CTCTAAATCG
GGGGCTCCCT
TTAGGGTTCC
GATTTAGTGC
TTTACGGCAC
CTCGACCCCA
AAAAACTTGA
TTAGGGTGAT
GGTTCACGTA
GTGGGCCATC
GCCCTGATAG
ACGGTTTTTC
GCCCTTTGAC
GTTGGAGTCC
ACGTTCTTTA
ATAGTGGACT
CTTGTTCCAA
ACTGGAACAA
CACTCAACCC
TATCTCGGTC
TATTCTTTTG
ATTTATAAGG
GATTTTGCCG
ATTTCGGCCT
ATTGGTTAAA
AAATGAGCTG
ATTTAACAAA
AATTTAACGC
GAATTTTAAC
AAAATATTAA
CGCTTACAAT
TTAGGTGGCA
CTTTTCGGGG
AAATGTGCGC
GGAACCCCTA
TTTGTTTATT
TTTCTAAATA
CATTCAAATA
TGTATCCGCT
CATGAGACAA
TAACCCTGAT
AAATGCTTCA
ATAATATTGA
AAAAGGAAGA
GTATGAGTAT
TCAACATTTC
CGTGTCGCCC
TTATTCCCTT
TTTTGCGGCA
TTTTGCCTTC
CTGTTTTTGC
TCACCCAGAA
ACGCTGGTGA
AAGTAAAAGA
TGCTGAAGAT
CAGTTGGGTG
CACGAGTGGG
TTACATCGAA
CTGGATCTCA
ACAGCGGTAA
GATCCTTGAG
AGTTTTCGCC
CCGAAGAACG
TTTTCCAATG
ATGAGCACTT
TTAAAGTTCT
GCTATGTGGC
GCGGTATTAT
CCCGTATTGA
CGCCGGGCAA
GAGCAACTCG
GTCGCCGCAT
ACACTATTCT
CAGAATGACT
TGGTTGAGTA
CTCACCAGTC
ACAGAAAAGC
ATCTTACGGA
TGGCATGACA
GTAAGAGAAT
TATGCAGTGC
TGCCATAACC
ATGAGTGATA
ACACTGCGGC
CAACTTACTT
CTGACAACGA
TCGGAGGACC
GAAGGAGCTA
ACCGCTTTTT
TGCACAACAT
GGGGGATCAT
GTAACTCGCC
TTGATCGTTG
GGAACCGGAG
CTGAATGAAG
CCATACCAAA
CGACGAGCGT
GACACCACGA
TGCCTGTAGC
AATGGCAACA
ACGTTGCGCA
AACTATTAAC
TGGCGAACTA
CTTACTCTAG
CTTCCCGGCA
ACAATTAATA
GACTGGATGG
AGGCGGATAA
AGTTGCAGGA
CCACTTCTGC
GCTCGGCCCT
TCCGGCTGGC
TGGTTTATTG
CTGATAAATC
TGGAGCCGGT
GAGCGTGGGT
CTCGCGGTAT
CATTGCAGCA
CTGGGGCCAG
ATGGTAAGCC
CTCCCGTATC
GTAGTTATCT
ACACGACGGG
GAGTCAGGCA
ACTATGGATG
AACGAAATAG
ACAGATCGCT
GAGATAGGTG
CCTCACTGAT
TAAGCATTGG
TAACTGTCAG
ACCAAGTTTA
CTCATATATA
CTTTAGATTG
ATTTAAAACT
TCATTTTTAA
TTTAAAAGGA
TCTAGGTGAA
GATCCTTTTT
GATAATCTCA
TGACCAAAAT
CCCTTAACGT
GAGTTTTCGT
TCCACTGAGC
GTCAGACCCC
GTAGAAAAGA
TCAAAGGATC
TTCTTGAGAT
CCTTTTTTTC
TGCGCGTAAT
CTGCTGCTTG
CAAACAAAAA
AACCACCGCT
ACCAGCGGTG
GTTTGTTTGC
CGGATCAAGA
GCTACCAACT
CTTTTTCCGA
AGGTAACTGG
CTTCAGCAGA
GCGCAGATAC
CAAATACTGT
TCTTCTAGTG
TAGCCGTAGT
TAGGCCACCA
CTTCAAGAAC
TCTGTAGCAC
CGCCTACATA
CCTCGCTCTG
CTAATCCTGT
TACCAGTGGC
TGCTGCCAGT
GGCGATAAGT
CGTGTCTTAC
CGGGTTGGAC
TCAAGACGAT
AGTTACCGGA
TAAGGCGCAG
CGGTCGGGCT
GAACGGGGGG
TTCGTGCACA
CAGCCCAGCT
TGGAGCGAAC
GACCTACACC
GAACTGAGAT
ACCTACAGCG
TGAGCTATGA
GAAAGCGCCA
CGCTTCCCGA
AGGGAGAAAG
GCGGACAGGT
ATCCGGTAAG
CGGCAGGGTC
GGAACAGGAG
AGCGCACGAG
GGAGCTTCCA
GGGGGAAACG
CCTGGTATCT
TTATAGTCCT
GTCGGGTTTC
GCCACCTCTG
ACTTGAGCGT
CGATTTTTGT
GATGCTCGTC
AGGGGGGCGG
AGCCTATGGA
AAAACGCCAG
CAACGCGGCC
TTTTTACGGT
TCCTGGCCTT
TTGCTGGCCT
TTTGCTCACA
TGTTCTTTCC
TGCGTTATCC
CCTGATTCTG
TGGATAACCG
TATTACCGCC
TTTGAGTGAG
CTGATACCGC
TCGCCGCAGC
CGAACGACCG
AGCGCAGCGA
GTCAGTGAGC
GAGGAAGCGG
AAGAGCGCCC
AATACGCAAA
CCGCCTCTCC
CCGCGCGTTG
GCCGATTCAT
TAATGCAGCT
GGCACGACAG
GTTTCCCGAC
TGGAAAGCGG
GCAGTGAGCG
CAACGCAATT
AATGTGAGTT
AGCTCACTCA
TTAGGCACCC
CAGGCTTTAC
ACTTTATGCT
TCCGGCTCGT
ATGTTGTGTG
GAATTGTGAG
CGGATAACAA
TTTCACACAG
GAAACAGCTA
TGACCATGAT
TACGCCAAGC
TCGAAATTAA
CCCTCACTAA
AGGGAACAAA
AGCTGGTACC
GGGCCCCCCC
TCGAGATCCG
GCCATTAGCC
ATATTATTCA
TTGGTTATAT
AGC