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pLV2-CMV-3×FLAG-Celf1(mouse)-Puro

产品货号:P51199

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pLV2-CMV-3×FLAG-Celf1(mouse)-Puro
别称: NM_017368.4;1600010O03Rik; Brunol2; CUG; CUG1;CUGBP; Cugbp1; D2Wsu101e; HNAB50; NAB50
基因长: 1539bp
质粒宿主: 哺乳细胞,慢病毒
质粒用途: 蛋白表达
片段类型: ORF
片段物种: 小鼠
原核抗性: Amp
筛选标记: Puro
荧光标记:
启动子: CMV
复制子: pUC
拷贝数:
感受态: Stbl3
温度: 37度
背景载体:
正向引物:
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

TGGAAGGGCT

AATTCACTCC

CAAAGAAGAC

AAGATATCCT

TGATCTGTGG

ATCTACCACA

61

CACAAGGCTA

CTTCCCTGAT

TAGCAGAACT

ACACACCAGG

GCCAGGGGTC

AGATATCCAC

121

TGACCTTTGG

ATGGTGCTAC

AAGCTAGTAC

CAGTTGAGCC

AGATAAGGTA

GAAGAGGCCA

181

ATAAAGGAGA

GAACACCAGC

TTGTTACACC

CTGTGAGCCT

GCATGGGATG

GATGACCCGG

241

AGAGAGAAGT

GTTAGAGTGG

AGGTTTGACA

GCCGCCTAGC

ATTTCATCAC

GTGGCCCGAG

301

AGCTGCATCC

GGAGTACTTC

AAGAACTGCT

GATATCGAGC

TTGCTACAAG

GGACTTTCCG

361

CTGGGGACTT

TCCAGGGAGG

CGTGGCCTGG

GCGGGACTGG

GGAGTGGCGA

GCCCTCAGAT

421

CCTGCATATA

AGCAGCTGCT

TTTTGCCTGT

ACTGGGTCTC

TCTGGTTAGA

CCAGATCTGA

481

GCCTGGGAGC

TCTCTGGCTA

ACTAGGGAAC

CCACTGCTTA

AGCCTCAATA

AAGCTTGCCT

541

TGAGTGCTTC

AAGTAGTGTG

TGCCCGTCTG

TTGTGTGACT

CTGGTAACTA

GAGATCCCTC

601

AGACCCTTTT

AGTCAGTGTG

GAAAATCTCT

AGCAGTGGCG

CCCGAACAGG

GACTTGAAAG

661

CGAAAGGGAA

ACCAGAGGAG

CTCTCTCGAC

GCAGGACTCG

GCTTGCTGAA

GCGCGCACGG

721

CAAGAGGCGA

GGGGCGGCGA

CTGGTGAGTA

CGCCAAAAAT

TTTGACTAGC

GGAGGCTAGA

781

AGGAGAGAGA

TGGGTGCGAG

AGCGTCAGTA

TTAAGCGGGG

GAGAATTAGA

TCGCGATGGG

841

AAAAAATTCG

GTTAAGGCCA

GGGGGAAAGA

AAAAATATAA

ATTAAAACAT

ATAGTATGGG

901

CAAGCAGGGA

GCTAGAACGA

TTCGCAGTTA

ATCCTGGCCT

GTTAGAAACA

TCAGAAGGCT

961

GTAGACAAAT

ACTGGGACAG

CTACAACCAT

CCCTTCAGAC

AGGATCAGAA

GAACTTAGAT

1021

CATTATATAA

TACAGTAGCA

ACCCTCTATT

GTGTGCATCA

AAGGATAGAG

ATAAAAGACA

1081

CCAAGGAAGC

TTTAGACAAG

ATAGAGGAAG

AGCAAAACAA

AAGTAAGACC

ACCGCACAGC

1141

AAGCGGCCGG

CCGCTGATCT

TCAGACCTGG

AGGAGGAGAT

ATGAGGGACA

ATTGGAGAAG

1201

TGAATTATAT

AAATATAAAG

TAGTAAAAAT

TGAACCATTA

GGAGTAGCAC

CCACCAAGGC

1261

AAAGAGAAGA

GTGGTGCAGA

GAGAAAAAAG

AGCAGTGGGA

ATAGGAGCTT

TGTTCCTTGG

1321

GTTCTTGGGA

GCAGCAGGAA

GCACTATGGG

CGCAGCGTCA

ATGACGCTGA

CGGTACAGGC

1381

CAGACAATTA

TTGTCTGGTA

TAGTGCAGCA

GCAGAACAAT

TTGCTGAGGG

CTATTGAGGC

1441

GCAACAGCAT

CTGTTGCAAC

TCACAGTCTG

GGGCATCAAG

CAGCTCCAGG

CAAGAATCCT

1501

GGCTGTGGAA

AGATACCTAA

AGGATCAACA

GCTCCTGGGG

ATTTGGGGTT

GCTCTGGAAA

1561

ACTCATTTGC

ACCACTGCTG

TGCCTTGGAA

TGCTAGTTGG

AGTAATAAAT

CTCTGGAACA

1621

GATTTGGAAT

CACACGACCT

GGATGGAGTG

GGACAGAGAA

ATTAACAATT

ACACAAGCTT

1681

AATACACTCC

TTAATTGAAG

AATCGCAAAA

CCAGCAAGAA

AAGAATGAAC

AAGAATTATT

1741

GGAATTAGAT

AAATGGGCAA

GTTTGTGGAA

TTGGTTTAAC

ATAACAAATT

GGCTGTGGTA

1801

TATAAAATTA

TTCATAATGA

TAGTAGGAGG

CTTGGTAGGT

TTAAGAATAG

TTTTTGCTGT

1861

ACTTTCTATA

GTGAATAGAG

TTAGGCAGGG

ATATTCACCA

TTATCGTTTC

AGACCCACCT

1921

CCCAACCCCG

AGGGGACCCG

ACAGGCCCGA

AGGAATAGAA

GAAGAAGGTG

GAGAGAGAGA

1981

CAGAGACAGA

TCCATTCGAT

TAGTGAACGG

ATCTCGACGG

TATCGCCTTT

AAAAGAAAAG

2041

GGGGGATTGG

GGGGTACAGT

GCAGGGGAAA

GAATAGTAGA

CATAATAGCA

ACAGACATAC

2101

AAACTAAAGA

ATTACAAAAA

CAAATTACAA

AAATTCAAAA

TTTTCGGGTT

TATTACAGGG

2161

ACAGCAGAGA

TCCAGTTTAT

CGATAAGCTT

GGGAGTTCCG

CGTTACATAA

CTTACGGTAA

2221

ATGGCCCGCC

TGGCTGACCG

CCCAACGACC

CCCGCCCATT

GACGTCAATA

ATGACGTATG

2281

TTCCCATAGT

AACGCCAATA

GGGACTTTCC

ATTGACGTCA

ATGGGTGGAG

TATTTACGGT

2341

AAACTGCCCA

CTTGGCAGTA

CATCAAGTGT

ATCATATGCC

AAGTACGCCC

CCTATTGACG

2401

TCAATGACGG

TAAATGGCCC

GCCTGGCATT

ATGCCCAGTA

CATGACCTTA

TGGGACTTTC

2461

CTACTTGGCA

GTACATCTAC

GTATTAGTCA

TCGCTATTAC

CATGGTGATG

CGGTTTTGGC

2521

AGTACATCAA

TGGGCGTGGA

TAGCGGTTTG

ACTCACGGGG

ATTTCCAAGT

CTCCACCCCA

2581

TTGACGTCAA

TGGGAGTTTG

TTTTGGCACC

AAAATCAACG

GGACTTTCCA

AAATGTCGTA

2641

ACAACTCCGC

CCCATTGACG

CAAATGGGCG

GTAGGCGTGT

ACGGTGGGAG

GTCTATATAA

2701

GCAGAGCTCG

TTTAGTGAAC

CGTCAGATCG

CCTGGAGACG

CCATCCACGC

TGTTTTGACC

2761

TCCATAGAAG

ACACCGACTC

TACTAGAGGA

TCGCTAGCGC

TACCGGACTC

AGATCTCGAG

2821

CCACCATGGA

CTACAAAGAC

CATGACGGTG

ATTATAAAGA

TCATGACATC

GACTACAAGG

2881

ATGACGATGA

CAAGGAATTC

ATGGCTGCGT

TTAAGTTGGA

TTTCCTTCCA

GAAATGATGG

2941

TGGATCATTG

CTCTTTGAAT

TCCAGTCCTG

TCTCAAAGAA

AATGAACGGC

ACCCTGGACC

3001

ACCCAGACCA

ACCAGATCTT

GATGCTATCA

AGATGTTTGT

GGGCCAGGTT

CCAAGGACCT

3061

GGTCTGAGAA

AGACTTGAGG

GAGCTTTTTG

AGCAGTATGG

TGCTGTCTAT

GAAATCAACA

3121

TCCTAAGGGA

TAGGAGCCAA

AACCCTCCTC

AGAGCAAAGG

GTGCTGTTTT

GTTACATTTT

3181

ACACCCGTAA

AGCTGCATTA

GAAGCTCAGA

ATGCTCTCCA

TAACATGAAG

GTCCTCCCTG

3241

GGATGCATCA

CCCTATACAG

ATGAAGCCTG

CTGACAGTGA

AAAGAACAAT

GCTGTGGAAG

3301

ACAGGAAGCT

CTTTATTGGT

ATGATTTCCA

AGAAGTGTAC

TGAAAATGAC

ATCCGAGTCA

3361

TGTTTTCTTC

GTTTGGACAG

ATTGAAGAGT

GCCGGATATT

GCGGGGACCT

GATGGCTTGA

3421

GCAGAGGTTG

TGCATTTGTG

ACTTTTACTA

CAAGAACCAT

GGCACAGACA

GCTATCAAAG

3481

CAATGCACCA

AGCACAAACC

ATGGAGGGTT

GCTCATCCCC

AATGGTGGTA

AAGTTTGCCG

3541

ACACCCAGAA

GGACAAAGAA

CAGAAGAGAA

TGGCCCAGCA

GCTCCAGCAG

CAGATGCAGC

3601

AGATCAGTGC

AGCGTCTGTG

TGGGGAAACC

TGGCTGGTCT

GAACACACTT

GGACCCCAGT

3661

ACTTAGCACT

TTATTTGCAG

CTCCTACAGC

AGACTGCCTC

CTCTGGGAAC

CTCAACACCC

3721

TGAGCAGCCT

CCACCCAATG

GGAGGGTTAA

ATGCAATGCA

GCTACAGAAT

TTGGCTGCAC

3781

TAGCTGCTGC

AGCTAGTGCA

GCTCAGAACA

CACCAAGTGG

TACCAATGCT

CTCACTACAT

3841

CCAGCAGTCC

CCTCAGCGTA

CTCACCAGTT

CAGGGTCTTC

ACCGAGCTCC

AGCAGCAGTA

3901

ATTCTGTCAA

CCCCATAGCC

TCACTTGGAG

CCCTGCAAAC

ATTGGCCGGA

GCAACAGCTG

3961

GCCTCAACGT

TGGCTCATTG

GCAGGGATGG

CTGCTTTGAA

TGGTGGTCTG

GGCAGCAGTG

4021

GCCTTTCCAA

TGGCACTGGG

AGTACCATGG

AAGCCCTCAC

CCAGGCGTAT

TCTGGTATCC

4081

AGCAATATGC

TGCTGCAGCC

CTCCCCACTC

TGTACAACCA

GAATTTATTG

ACACAGCAGA

4141

GTATTGGTGC

TGCTGGAAGC

CAGAAGGAAG

GTCCAGAAGG

AGCCAACCTG

TTCATCTATC

4201

ACCTGCCCCA

GGAGTTTGGA

GACCAGGACT

TACTGCAGAT

GTTTATGCCC

TTTGGGAATG

4261

TCGTGTCGGC

CAAGGTTTTC

ATAGACAAGC

AGACAAACCT

GAGCAAGTGC

TTTGGTTTTG

4321

TAAGTTATGA

CAATCCTGTC

TCAGCTCAAG

CTGCCATCCA

GTCCATGAAC

GGCTTTCAAA

4381

TTGGAATGAA

GAGGCTTAAA

GTGCAGCTCA

AACGTTCGAA

GAATGATAGT

AAGCCCTACG

4441

GATCCCGCGA

CTCTAGATAA

TTCTACCGGG

TAGGGGAGGC

GCTTTTCCCA

AGGCAGTCTG

4501

GAGCATGCGC

TTTAGCAGCC

CCGCTGGGCA

CTTGGCGCTA

CACAAGTGGC

CTCTGGCCTC

4561

GCACACATTC

CACATCCACC

GGTAGGCGCC

AACCGGCTCC

GTTCTTTGGT

GGCCCCTTCG

4621

CGCCACCTTC

TACTCCTCCC

CTAGTCAGGA

AGTTCCCCCC

CGCCCCGCAG

CTCGCGTCGT

4681

GCAGGACGTG

ACAAATGGAA

GTAGCACGTC

TCACTAGTCT

CGTGCAGATG

GACAGCACCG

4741

CTGAGCAATG

GAAGCGGGTA

GGCCTTTGGG

GCAGCGGCCA

ATAGCAGCTT

TGCTCCTTCG

4801

CTTTCTGGGC

TCAGAGGCTG

GGAAGGGGTG

GGTCCGGGGG

CGGGCTCAGG

GGCGGGCTCA

4861

GGGGCGGGGC

GGGCGCCCGA

AGGTCCTCCG

GAGGCCCGGC

ATTCTGCACG

CTTCAAAAGC

4921

GCACGTCTGC

CGCGCTGTTC

TCCTCTTCCT

CATCTCCGGG

CCTTTCGACC

TGCAGCCCAA

4981

GCTTACCATG

ACCGAGTACA

AGCCCACGGT

GCGCCTCGCC

ACCCGCGACG

ACGTCCCCAG

5041

GGCCGTACGC

ACCCTCGCCG

CCGCGTTCGC

CGACTACCCC

GCCACGCGCC

ACACCGTCGA

5101

TCCGGACCGC

CACATCGAGC

GGGTCACCGA

GCTGCAAGAA

CTCTTCCTCA

CGCGCGTCGG

5161

GCTCGACATC

GGCAAGGTGT

GGGTCGCGGA

CGACGGCGCC

GCGGTGGCGG

TCTGGACCAC

5221

GCCGGAGAGC

GTCGAAGCGG

GGGCGGTGTT

CGCCGAGATC

GGCCCGCGCA

TGGCCGAGTT

5281

GAGCGGTTCC

CGGCTGGCCG

CGCAGCAACA

GATGGAAGGC

CTCCTGGCGC

CGCACCGGCC

5341

CAAGGAGCCC

GCGTGGTTCC

TGGCCACCGT

CGGCGTCTCG

CCCGACCACC

AGGGCAAGGG

5401

TCTGGGCAGC

GCCGTCGTGC

TCCCCGGAGT

GGAGGCGGCC

GAGCGCGCCG

GGGTGCCCGC

5461

CTTCCTGGAG

ACCTCCGCGC

CCCGCAACCT

CCCCTTCTAC

GAGCGGCTCG

GCTTCACCGT

5521

CACCGCCGAC

GTCGAGGTGC

CCGAAGGACC

GCGCACCTGG

TGCATGACCC

GCAAGCCCGG

5581

TGCCTGACCG

CGTCTGGAAC

AATCAACCTC

TGGATTACAA

AATTTGTGAA

AGATTGACTG

5641

GTATTCTTAA

CTATGTTGCT

CCTTTTACGC

TATGTGGATA

CGCTGCTTTA

ATGCCTTTGT

5701

ATCATGCTAT

TGCTTCCCGT

ATGGCTTTCA

TTTTCTCCTC

CTTGTATAAA

TCCTGGTTGC

5761

TGTCTCTTTA

TGAGGAGTTG

TGGCCCGTTG

TCAGGCAACG

TGGCGTGGTG

TGCACTGTGT

5821

TTGCTGACGC

AACCCCCACT

GGTTGGGGCA

TTGCCACCAC

CTGTCAGCTC

CTTTCCGGGA

5881

CTTTCGCTTT

CCCCCTCCCT

ATTGCCACGG

CGGAACTCAT

CGCCGCCTGC

CTTGCCCGCT

5941

GCTGGACAGG

GGCTCGGCTG

TTGGGCACTG

ACAATTCCGT

GGTGTTGTCG

GGGAAGCTGA

6001

CGTCCTTTCC

ATGGCTGCTC

GCCTGTGTTG

CCACCTGGAT

TCTGCGCGGG

ACGTCCTTCT

6061

GCTACGTCCC

TTCGGCCCTC

AATCCAGCGG

ACCTTCCTTC

CCGCGGCCTG

CTGCCGGCTC

6121

TGCGGCCTCT

TCCGCGTCTT

CGCCTTCGCC

CTCAGACGAG

TCGGATCTCC

CTTTGGGCCG

6181

CCTCCCCGCC

TGGAATTAAT

TCTGCAGTCG

AGACCTAGAA

AAACATGGAG

CAATCACAAG

6241

TAGCAATACA

GCAGCTACCA

ATGCTGATTG

TGCCTGGCTA

GAAGCACAAG

AGGAGGAGGA

6301

GGTGGGTTTT

CCAGTCACAC

CTCAGGTACC

TTTAAGACCA

ATGACTTACA

AGGCAGCTGT

6361

AGATCTTAGC

CACTTTTTAA

AAGAAAAGAG

GGGACTGGAA

GGGCTAATTC

ACTCCCAACG

6421

AAGACAAGAT

ATCCTTGATC

TGTGGATCTA

CCACACACAA

GGCTACTTCC

CTGATTAGCA

6481

GAACTACACA

CCAGGGCCAG

GGGTCAGATA

TCCACTGACC

TTTGGATGGT

GCTACAAGCT

6541

AGTACCAGTT

GAGCCAGATA

AGGTAGAAGA

GGCCAATAAA

GGAGAGAACA

CCAGCTTGTT

6601

ACACCCTGTG

AGCCTGCATG

GGATGGATGA

CCCGGAGAGA

GAAGTGTTAG

AGTGGAGGTT

6661

TGACAGCCGC

CTAGCATTTC

ATCACGTGGC

CCGAGAGCTG

CATCCGGAGT

ACTTCAAGAA

6721

CTGCTGATAT

CGAGCTTGCT

ACAAGGGACT

TTCCGCTGGG

GACTTTCCAG

GGAGGCGTGG

6781

CCTGGGCGGG

ACTGGGGAGT

GGCGAGCCCT

CAGATCCTGC

ATATAAGCAG

CTGCTTTTTG

6841

CCTGTACTGG

GTCTCTCTGG

TTAGACCAGA

TCTGAGCCTG

GGAGCTCTCT

GGCTAACTAG

6901

GGAACCCACT

GCTTAAGCCT

CAATAAAGCT

TGCCTTGAGT

GCTTCAAGTA

GTGTGTGCCC

6961

GTCTGTTGTG

TGACTCTGGT

AACTAGAGAT

CCCTCAGACC

CTTTTAGTCA

GTGTGGAAAA

7021

TCTCTAGCAG

TAGTAGTTCA

TGTCATCTTA

TTATTCAGTA

TTTATAACTT

GCAAAGAAAT

7081

GAATATCAGA

GAGTGAGAGG

CCTTGACATT

GCTAGCGTTT

TACCGTCGAC

CTCTAGCTAG

7141

AGCTTGGCGT

AATCATGGTC

ATAGCTGTTT

CCTGTGTGAA

ATTGTTATCC

GCTCACAATT

7201

CCACACAACA

TACGAGCCGG

AAGCATAAAG

TGTAAAGCCT

GGGGTGCCTA

ATGAGTGAGC

7261

TAACTCACAT

TAATTGCGTT

GCGCTCACTG

CCCGCTTTCC

AGTCGGGAAA

CCTGTCGTGC

7321

CAGCTGCATT

AATGAATCGG

CCAACGCGCG

GGGAGAGGCG

GTTTGCGTAT

TGGGCGCTCT

7381

TCCGCTTCCT

CGCTCACTGA

CTCGCTGCGC

TCGGTCGTTC

GGCTGCGGCG

AGCGGTATCA

7441

GCTCACTCAA

AGGCGGTAAT

ACGGTTATCC

ACAGAATCAG

GGGATAACGC

AGGAAAGAAC

7501

ATGTGAGCAA

AAGGCCAGCA

AAAGGCCAGG

AACCGTAAAA

AGGCCGCGTT

GCTGGCGTTT

7561

TTCCATAGGC

TCCGCCCCCC

TGACGAGCAT

CACAAAAATC

GACGCTCAAG

TCAGAGGTGG

7621

CGAAACCCGA

CAGGACTATA

AAGATACCAG

GCGTTTCCCC

CTGGAAGCTC

CCTCGTGCGC

7681

TCTCCTGTTC

CGACCCTGCC

GCTTACCGGA

TACCTGTCCG

CCTTTCTCCC

TTCGGGAAGC

7741

GTGGCGCTTT

CTCATAGCTC

ACGCTGTAGG

TATCTCAGTT

CGGTGTAGGT

CGTTCGCTCC

7801

AAGCTGGGCT

GTGTGCACGA

ACCCCCCGTT

CAGCCCGACC

GCTGCGCCTT

ATCCGGTAAC

7861

TATCGTCTTG

AGTCCAACCC

GGTAAGACAC

GACTTATCGC

CACTGGCAGC

AGCCACTGGT

7921

AACAGGATTA

GCAGAGCGAG

GTATGTAGGC

GGTGCTACAG

AGTTCTTGAA

GTGGTGGCCT

7981

AACTACGGCT

ACACTAGAAG

AACAGTATTT

GGTATCTGCG

CTCTGCTGAA

GCCAGTTACC

8041

TTCGGAAAAA

GAGTTGGTAG

CTCTTGATCC

GGCAAACAAA

CCACCGCTGG

TAGCGGTGGT

8101

TTTTTTGTTT

GCAAGCAGCA

GATTACGCGC

AGAAAAAAAG

GATCTCAAGA

AGATCCTTTG

8161

ATCTTTTCTA

CGGGGTCTGA

CGCTCAGTGG

AACGAAAACT

CACGTTAAGG

GATTTTGGTC

8221

ATGAGATTAT

CAAAAAGGAT

CTTCACCTAG

ATCCTTTTAA

ATTAAAAATG

AAGTTTTAAA

8281

TCAATCTAAA

GTATATATGA

GTAAACTTGG

TCTGACAGTT

ACCAATGCTT

AATCAGTGAG

8341

GCACCTATCT

CAGCGATCTG

TCTATTTCGT

TCATCCATAG

TTGCCTGACT

CCCCGTCGTG

8401

TAGATAACTA

CGATACGGGA

GGGCTTACCA

TCTGGCCCCA

GTGCTGCAAT

GATACCGCGA

8461

GACCCACGCT

CACCGGCTCC

AGATTTATCA

GCAATAAACC

AGCCAGCCGG

AAGGGCCGAG

8521

CGCAGAAGTG

GTCCTGCAAC

TTTATCCGCC

TCCATCCAGT

CTATTAATTG

TTGCCGGGAA

8581

GCTAGAGTAA

GTAGTTCGCC

AGTTAATAGT

TTGCGCAACG

TTGTTGCCAT

TGCTACAGGC

8641

ATCGTGGTGT

CACGCTCGTC

GTTTGGTATG

GCTTCATTCA

GCTCCGGTTC

CCAACGATCA

8701

AGGCGAGTTA

CATGATCCCC

CATGTTGTGC

AAAAAAGCGG

TTAGCTCCTT

CGGTCCTCCG

8761

ATCGTTGTCA

GAAGTAAGTT

GGCCGCAGTG

TTATCACTCA

TGGTTATGGC

AGCACTGCAT

8821

AATTCTCTTA

CTGTCATGCC

ATCCGTAAGA

TGCTTTTCTG

TGACTGGTGA

GTACTCAACC

8881

AAGTCATTCT

GAGAATAGTG

TATGCGGCGA

CCGAGTTGCT

CTTGCCCGGC

GTCAATACGG

8941

GATAATACCG

CGCCACATAG

CAGAACTTTA

AAAGTGCTCA

TCATTGGAAA

ACGTTCTTCG

9001

GGGCGAAAAC

TCTCAAGGAT

CTTACCGCTG

TTGAGATCCA

GTTCGATGTA

ACCCACTCGT

9061

GCACCCAACT

GATCTTCAGC

ATCTTTTACT

TTCACCAGCG

TTTCTGGGTG

AGCAAAAACA

9121

GGAAGGCAAA

ATGCCGCAAA

AAAGGGAATA

AGGGCGACAC

GGAAATGTTG

AATACTCATA

9181

CTCTTCCTTT

TTCAATATTA

TTGAAGCATT

TATCAGGGTT

ATTGTCTCAT

GAGCGGATAC

9241

ATATTTGAAT

GTATTTAGAA

AAATAAACAA

ATAGGGGTTC

CGCGCACATT

TCCCCGAAAA

9301

GTGCCACCTG

ACGTCGACGG

ATCGGGAGAT

CAACTTGTTT

ATTGCAGCTT

ATAATGGTTA

9361

CAAATAAAGC

AATAGCATCA

CAAATTTCAC

AAATAAAGCA

TTTTTTTCAC

TGCATTCTAG

9421

TTGTGGTTTG

TCCAAACTCA

TCAATGTATC

TTATCATGTC

TGGATCAACT

GGATAACTCA

9481

AGCTAACCAA

AATCATCCCA

AACTTCCCAC

CCCATACCCT

ATTACCACTG

CCAATTACCT

9541

GTGGTTTCAT

TTACTCTAAA

CCTGTGATTC

CTCTGAATTA

TTTTCATTTT

AAAGAAATTG

9601

TATTTGTTAA

ATATGTACTA

CAAACTTAGT

AGTTTTTAAA

GAAATTGTAT

TTGTTAAATA

9661

TGTACTACAA

ACTTAGTAGT

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