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FokI-dCas9

产品货号:P5109

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: FokI-dCas9
别称: Plasmid#52970
基因长:
质粒宿主: 哺乳细胞
质粒用途: 基因编辑
片段类型: CRISPR
片段物种:
原核抗性: Amp
筛选标记:
荧光标记:
启动子:
复制子:
拷贝数:
感受态: Stbl3
温度: 37度
背景载体:
正向引物:
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

ATATGCCAAG

TACGCCCCCT

ATTGACGTCA

ATGACGGTAA

ATGGCCCGCC

TGGCATTATG

61

CCCAGTACAT

GACCTTATGG

GACTTTCCTA

CTTGGCAGTA

CATCTACGTA

TTAGTCATCG

121

CTATTACCAT

GGTGATGCGG

TTTTGGCAGT

ACATCAATGG

GCGTGGATAG

CGGTTTGACT

181

CACGGGGATT

TCCAAGTCTC

CACCCCATTG

ACGTCAATGG

GAGTTTGTTT

TGGCACCAAA

241

ATCAACGGGA

CTTTCCAAAA

TGTCGTAACA

ACTCCGCCCC

ATTGACGCAA

ATGGGCGGTA

301

GGCGTGTACG

GTGGGAGGTC

TATATAAGCA

GAGCTGGTTT

AGTGAACCGT

CAGATCCGCT

361

AGAGATCCGC

GGCCGCTAAT

ACGACTCACT

ATAGGGAGAG

CCGCCACCAT

GGACTACAAA

421

GACCATGACG

GTGATTATAA

AGATCATGAC

ATCGATTACA

AGGATGACGA

TGACAAGATG

481

GCCCCCAAGA

AGAAGAGGAA

GGTGGGCATT

CACCGCGGGG

TACCTGGAGG

TTCTGGATCC

541

CAACTAGTCA

AAAGTGAACT

GGAGGAGAAG

AAATCTGAAC

TTCGTCATAA

ATTGAAATAT

601

GTGCCTCATG

AATATATTGA

ATTAATTGAA

ATTGCCAGAA

ATTCCACTCA

GGATAGAATT

661

CTTGAAATGA

AGGTAATGGA

ATTTTTTATG

AAAGTTTATG

GATATAGAGG

TAAACATTTG

721

GGTGGATCAA

GGAAACCGGA

CGGAGCAATT

TATACTGTCG

GATCTCCTAT

TGATTACGGT

781

GTGATCGTGG

ATACTAAAGC

TTATAGCGGA

GGTTATAATC

TGCCAATTGG

CCAAGCAGAT

841

GAAATGCAAC

GATATGTCGA

AGAAAATCAA

ACACGAAACA

AACATATCAA

CCCTAATGAA

901

TGGTGGAAAG

TCTATCCATC

TTCTGTAACG

GAATTTAAGT

TTTTATTTGT

GAGTGGTCAC

961

TTTAAAGGAA

ACTACAAAGC

TCAGCTTACA

CGATTAAATC

ATATCACTAA

TTGTAATGGA

1021

GCTGTTCTTA

GTGTAGAAGA

GCTTTTAATT

GGTGGAGAAA

TGATTAAAGC

CGGCACATTA

1081

ACCTTAGAGG

AAGTCAGACG

GAAATTTAAT

AACGGCGAGA

TAAACTTTAG

CGGCAGCGAG

1141

ACTCCCGGGA

CCTCAGAGTC

CGCCACACCC

GAAAGTGATA

AAAAGTATTC

TATTGGTTTA

1201

GCTATCGGCA

CTAATTCCGT

TGGATGGGCT

GTCATAACCG

ATGAATACAA

AGTACCTTCA

1261

AAGAAATTTA

AGGTGTTGGG

GAACACAGAC

CGTCATTCGA

TTAAAAAGAA

TCTTATCGGT

1321

GCCCTCCTAT

TCGATAGTGG

CGAAACGGCA

GAGGCGACTC

GCCTGAAACG

AACCGCTCGG

1381

AGAAGGTATA

CACGTCGCAA

GAACCGAATA

TGTTACTTAC

AAGAAATTTT

TAGCAATGAG

1441

ATGGCCAAAG

TTGACGATTC

TTTCTTTCAC

CGTTTGGAAG

AGTCCTTCCT

TGTCGAAGAG

1501

GACAAGAAAC

ATGAACGGCA

CCCCATCTTT

GGAAACATAG

TAGATGAGGT

GGCATATCAT

1561

GAAAAGTACC

CAACGATTTA

TCACCTCAGA

AAAAAGCTAG

TTGACTCAAC

TGATAAAGCG

1621

GACCTGAGGT

TAATCTACTT

GGCTCTTGCC

CATATGATAA

AGTTCCGTGG

GCACTTTCTC

1681

ATTGAGGGTG

ATCTAAATCC

GGACAACTCG

GATGTCGACA

AACTGTTCAT

CCAGTTAGTA

1741

CAAACCTATA

ATCAGTTGTT

TGAAGAGAAC

CCTATAAATG

CAAGTGGCGT

GGATGCGAAG

1801

GCTATTCTTA

GCGCCCGCCT

CTCTAAATCC

CGACGGCTAG

AAAACCTGAT

CGCACAATTA

1861

CCCGGAGAGA

AGAAAAATGG

GTTGTTCGGT

AACCTTATAG

CGCTCTCACT

AGGCCTGACA

1921

CCAAATTTTA

AGTCGAACTT

CGACTTAGCT

GAAGATGCCA

AATTGCAGCT

TAGTAAGGAC

1981

ACGTACGATG

ACGATCTCGA

CAATCTACTG

GCACAAATTG

GAGATCAGTA

TGCGGACTTA

2041

TTTTTGGCTG

CCAAAAACCT

TAGCGATGCA

ATCCTCCTAT

CTGACATACT

GAGAGTTAAT

2101

ACTGAGATTA

CCAAGGCGCC

GTTATCCGCT

TCAATGATCA

AAAGGTACGA

TGAACATCAC

2161

CAAGACTTGA

CACTTCTCAA

GGCCCTAGTC

CGTCAGCAAC

TGCCTGAGAA

ATATAAGGAA

2221

ATATTCTTTG

ATCAGTCGAA

AAACGGGTAC

GCAGGTTATA

TTGACGGCGG

AGCGAGTCAA

2281

GAGGAATTCT

ACAAGTTTAT

CAAACCCATA

TTAGAGAAGA

TGGATGGGAC

GGAAGAGTTG

2341

CTTGTAAAAC

TCAATCGCGA

AGATCTACTG

CGAAAGCAGC

GGACTTTCGA

CAACGGTAGC

2401

ATTCCACATC

AAATCCACTT

AGGCGAATTG

CATGCTATAC

TTAGAAGGCA

GGAGGATTTT

2461

TATCCGTTCC

TCAAAGACAA

TCGTGAAAAG

ATTGAGAAAA

TCCTAACCTT

TCGCATACCT

2521

TACTATGTGG

GACCCCTGGC

CCGAGGGAAC

TCTCGGTTCG

CATGGATGAC

AAGAAAGTCC

2581

GAAGAAACGA

TTACTCCATG

GAATTTTGAG

GAAGTTGTCG

ATAAAGGTGC

GTCAGCTCAA

2641

TCGTTCATCG

AGAGGATGAC

CAACTTTGAC

AAGAATTTAC

CGAACGAAAA

AGTATTGCCT

2701

AAGCACAGTT

TACTTTACGA

GTATTTCACA

GTGTACAATG

AACTCACGAA

AGTTAAGTAT

2761

GTCACTGAGG

GCATGCGTAA

ACCCGCCTTT

CTAAGCGGAG

AACAGAAGAA

AGCAATAGTA

2821

GATCTGTTAT

TCAAGACCAA

CCGCAAAGTG

ACAGTTAAGC

AATTGAAAGA

GGACTACTTT

2881

AAGAAAATTG

AATGCTTCGA

TTCTGTCGAG

ATCTCCGGGG

TAGAAGATCG

ATTTAATGCG

2941

TCACTTGGTA

CGTATCATGA

CCTCCTAAAG

ATAATTAAAG

ATAAGGACTT

CCTGGATAAC

3001

GAAGAGAATG

AAGATATCTT

AGAAGATATA

GTGTTGACTC

TTACCCTCTT

TGAAGATCGG

3061

GAAATGATTG

AGGAAAGACT

AAAAACATAC

GCTCACCTGT

TCGACGATAA

GGTTATGAAA

3121

CAGTTAAAGA

GGCGTCGCTA

TACGGGCTGG

GGACGATTGT

CGCGGAAACT

TATCAACGGG

3181

ATAAGAGACA

AGCAAAGTGG

TAAAACTATT

CTCGATTTTC

TAAAGAGCGA

CGGCTTCGCC

3241

AATAGGAACT

TTATGCAGCT

GATCCATGAT

GACTCTTTAA

CCTTCAAAGA

GGATATACAA

3301

AAGGCACAGG

TTTCCGGACA

AGGGGACTCA

TTGCACGAAC

ATATTGCGAA

TCTTGCTGGT

3361

TCGCCAGCCA

TCAAAAAGGG

CATACTCCAG

ACAGTCAAAG

TAGTGGATGA

GCTAGTTAAG

3421

GTCATGGGAC

GTCACAAACC

GGAAAACATT

GTAATCGAGA

TGGCACGCGA

AAATCAAACG

3481

ACTCAGAAGG

GGCAAAAAAA

CAGTCGAGAG

CGGATGAAGA

GAATAGAAGA

GGGTATTAAA

3541

GAACTGGGCA

GCCAGATCTT

AAAGGAGCAT

CCTGTGGAAA

ATACCCAATT

GCAGAACGAG

3601

AAACTTTACC

TCTATTACCT

ACAAAATGGA

AGGGACATGT

ATGTTGATCA

GGAACTGGAC

3661

ATAAACCGTT

TATCTGATTA

CGACGTCGAT

GCCATTGTAC

CCCAATCCTT

TTTGAAGGAC

3721

GATTCAATCG

ACAATAAAGT

GCTTACACGC

TCGGATAAGA

ACCGAGGGAA

AAGTGACAAT

3781

GTTCCAAGCG

AGGAAGTCGT

AAAGAAAATG

AAGAACTATT

GGCGGCAGCT

CCTAAATGCG

3841

AAACTGATAA

CGCAAAGAAA

GTTCGATAAC

TTAACTAAAG

CTGAGAGGGG

TGGCTTGTCT

3901

GAACTTGACA

AGGCCGGATT

TATTAAACGT

CAGCTCGTGG

AAACCCGCCA

AATCACAAAG

3961

CATGTTGCAC

AGATACTAGA

TTCCCGAATG

AATACGAAAT

ACGACGAGAA

CGATAAGCTG

4021

ATTCGGGAAG

TCAAAGTAAT

CACTTTAAAG

TCAAAATTGG

TGTCGGACTT

CAGAAAGGAT

4081

TTTCAATTCT

ATAAAGTTAG

GGAGATAAAT

AACTACCACC

ATGCGCACGA

CGCTTATCTT

4141

AATGCCGTCG

TAGGGACCGC

ACTCATTAAG

AAATACCCGA

AGCTAGAAAG

TGAGTTTGTG

4201

TATGGTGATT

ACAAAGTTTA

TGACGTCCGT

AAGATGATCG

CGAAAAGCGA

ACAGGAGATA

4261

GGCAAGGCTA

CAGCCAAATA

CTTCTTTTAT

TCTAACATTA

TGAATTTCTT

TAAGACGGAA

4321

ATCACTCTGG

CAAACGGAGA

GATACGCAAA

CGACCTTTAA

TTGAAACCAA

TGGGGAGACA

4381

GGTGAAATCG

TATGGGATAA

GGGCCGGGAC

TTCGCGACGG

TGAGAAAAGT

TTTGTCCATG

4441

CCCCAAGTCA

ACATAGTAAA

GAAAACTGAG

GTGCAGACCG

GAGGGTTTTC

AAAGGAATCG

4501

ATTCTTCCAA

AAAGGAATAG

TGATAAGCTC

ATCGCTCGTA

AAAAGGACTG

GGACCCGAAA

4561

AAGTACGGTG

GCTTCGATAG

CCCTACAGTT

GCCTATTCTG

TCCTAGTAGT

GGCAAAAGTT

4621

GAGAAGGGAA

AATCCAAGAA

ACTGAAGTCA

GTCAAAGAAT

TATTGGGGAT

AACGATTATG

4681

GAGCGCTCGT

CTTTTGAAAA

GAACCCCATC

GACTTCCTTG

AGGCGAAAGG

TTACAAGGAA

4741

GTAAAAAAGG

ATCTCATAAT

TAAACTACCA

AAGTATAGTC

TGTTTGAGTT

AGAAAATGGC

4801

CGAAAACGGA

TGTTGGCTAG

CGCCGGAGAG

CTTCAAAAGG

GGAACGAACT

CGCACTACCG

4861

TCTAAATACG

TGAATTTCCT

GTATTTAGCG

TCCCATTACG

AGAAGTTGAA

AGGTTCACCT

4921

GAAGATAACG

AACAGAAGCA

ACTTTTTGTT

GAGCAGCACA

AACATTATCT

CGACGAAATC

4981

ATAGAGCAAA

TTTCGGAATT

CAGTAAGAGA

GTCATCCTAG

CTGATGCCAA

TCTGGACAAA

5041

GTATTAAGCG

CATACAACAA

GCACAGGGAT

AAACCCATAC

GTGAGCAGGC

GGAAAATATT

5101

ATCCATTTGT

TTACTCTTAC

CAACCTCGGC

GCTCCAGCCG

CATTCAAGTA

TTTTGACACA

5161

ACGATAGATC

GCAAACGATA

CACTTCTACC

AAGGAGGTGC

TAGACGCGAC

ACTGATTCAC

5221

CAATCCATCA

CGGGATTATA

TGAAACTCGG

ATAGATTTGT

CACAGCTTGG

GGGTGACTGA

5281

CCGGTCATCA

TCACCATCAC

CATTGAGTTT

AAACCCGCTG

ATCAGCCTCG

ACTGTGCCTT

5341

CTAGTTGCCA

GCCATCTGTT

GTTTGCCCCT

CCCCCGTGCC

TTCCTTGACC

CTGGAAGGTG

5401

CCACTCCCAC

TGTCCTTTCC

TAATAAAATG

AGGAAATTGC

ATCGCATTGT

CTGAGTAGGT

5461

GTCATTCTAT

TCTGGGGGGT

GGGGTGGGGC

AGGACAGCAA

GGGGGAGGAT

TGGGAAGACA

5521

ATAGCAGGCA

TGCTGGGGAT

GCGGTGGGCT

CTATGGCTTC

TGAGGCGGAA

AGAACCAGCT

5581

GGGGCTCGAT

ACCGTCGACC

TCTAGCTAGA

GCTTGGCGTA

ATCATGGTCA

TAGCTGTTTC

5641

CTGTGTGAAA

TTGTTATCCG

CTCACAATTC

CACACAACAT

ACGAGCCGGA

AGCATAAAGT

5701

GTAAAGCCTA

GGGTGCCTAA

TGAGTGAGCT

AACTCACATT

AATTGCGTTG

CGCTCACTGC

5761

CCGCTTTCCA

GTCGGGAAAC

CTGTCGTGCC

AGCTGCATTA

ATGAATCGGC

CAACGCGCGG

5821

GGAGAGGCGG

TTTGCGTATT

GGGCGCTCTT

CCGCTTCCTC

GCTCACTGAC

TCGCTGCGCT

5881

CGGTCGTTCG

GCTGCGGCGA

GCGGTATCAG

CTCACTCAAA

GGCGGTAATA

CGGTTATCCA

5941

CAGAATCAGG

GGATAACGCA

GGAAAGAACA

TGTGAGCAAA

AGGCCAGCAA

AAGGCCAGGA

6001

ACCGTAAAAA

GGCCGCGTTG

CTGGCGTTTT

TCCATAGGCT

CCGCCCCCCT

GACGAGCATC

6061

ACAAAAATCG

ACGCTCAAGT

CAGAGGTGGC

GAAACCCGAC

AGGACTATAA

AGATACCAGG

6121

CGTTTCCCCC

TGGAAGCTCC

CTCGTGCGCT

CTCCTGTTCC

GACCCTGCCG

CTTACCGGAT

6181

ACCTGTCCGC

CTTTCTCCCT

TCGGGAAGCG

TGGCGCTTTC

TCAATGCTCA

CGCTGTAGGT

6241

ATCTCAGTTC

GGTGTAGGTC

GTTCGCTCCA

AGCTGGGCTG

TGTGCACGAA

CCCCCCGTTC

6301

AGCCCGACCG

CTGCGCCTTA

TCCGGTAACT

ATCGTCTTGA

GTCCAACCCG

GTAAGACACG

6361

ACTTATCGCC

ACTGGCAGCA

GCCACTGGTA

ACAGGATTAG

CAGAGCGAGG

TATGTAGGCG

6421

GTGCTACAGA

GTTCTTGAAG

TGGTGGCCTA

ACTACGGCTA

CACTAGAAGG

ACAGTATTTG

6481

GTATCTGCGC

TCTGCTGAAG

CCAGTTACCT

TCGGAAAAAG

AGTTGGTAGC

TCTTGATCCG

6541

GCAAACAAAC

CACCGCTGGT

AGCGGTGGTT

TTTTTGTTTG

CAAGCAGCAG

ATTACGCGCA

6601

GAAAAAAAGG

ATCTCAAGAA

GATCCTTTGA

TCTTTTCTAC

GGGGTCTGAC

GCTCAGTGGA

6661

ACGAAAACTC

ACGTTAAGGG

ATTTTGGTCA

TGAGATTATC

AAAAAGGATC

TTCACCTAGA

6721

TCCTTTTAAA

TTAAAAATGA

AGTTTTAAAT

CAATCTAAAG

TATATATGAG

TAAACTTGGT

6781

CTGACAGTTA

CCAATGCTTA

ATCAGTGAGG

CACCTATCTC

AGCGATCTGT

CTATTTCGTT

6841

CATCCATAGT

TGCCTGACTC

CCCGTCGTGT

AGATAACTAC

GATACGGGAG

GGCTTACCAT

6901

CTGGCCCCAG

TGCTGCAATG

ATACCGCGAG

ACCCACGCTC

ACCGGCTCCA

GATTTATCAG

6961

CAATAAACCA

GCCAGCCGGA

AGGGCCGAGC

GCAGAAGTGG

TCCTGCAACT

TTATCCGCCT

7021

CCATCCAGTC

TATTAATTGT

TGCCGGGAAG

CTAGAGTAAG

TAGTTCGCCA

GTTAATAGTT

7081

TGCGCAACGT

TGTTGCCATT

GCTACAGGCA

TCGTGGTGTC

ACGCTCGTCG

TTTGGTATGG

7141

CTTCATTCAG

CTCCGGTTCC

CAACGATCAA

GGCGAGTTAC

ATGATCCCCC

ATGTTGTGCA

7201

AAAAAGCGGT

TAGCTCCTTC

GGTCCTCCGA

TCGTTGTCAG

AAGTAAGTTG

GCCGCAGTGT

7261

TATCACTCAT

GGTTATGGCA

GCACTGCATA

ATTCTCTTAC

TGTCATGCCA

TCCGTAAGAT

7321

GCTTTTCTGT

GACTGGTGAG

TACTCAACCA

AGTCATTCTG

AGAATAGTGT

ATGCGGCGAC

7381

CGAGTTGCTC

TTGCCCGGCG

TCAATACGGG

ATAATACCGC

GCCACATAGC

AGAACTTTAA

7441

AAGTGCTCAT

CATTGGAAAA

CGTTCTTCGG

GGCGAAAACT

CTCAAGGATC

TTACCGCTGT

7501

TGAGATCCAG

TTCGATGTAA

CCCACTCGTG

CACCCAACTG

ATCTTCAGCA

TCTTTTACTT

7561

TCACCAGCGT

TTCTGGGTGA

GCAAAAACAG

GAAGGCAAAA

TGCCGCAAAA

AAGGGAATAA

7621

GGGCGACACG

GAAATGTTGA

ATACTCATAC

TCTTCCTTTT

TCAATATTAT

TGAAGCATTT

7681

ATCAGGGTTA

TTGTCTCATG

AGCGGATACA

TATTTGAATG

TATTTAGAAA

AATAAACAAA

7741

TAGGGGTTCC

GCGCACATTT

CCCCGAAAAG

TGCCACCTGA

CGTCGACGGA

TCGGGAGATC

7801

GATCTCCCGA

TCCCCTAGGG

TCGACTCTCA

GTACAATCTG

CTCTGATGCC

GCATAGTTAA

7861

GCCAGTATCT

GCTCCCTGCT

TGTGTGTTGG

AGGTCGCTGA

GTAGTGCGCG

AGCAAAATTT

7921

AAGCTACAAC

AAGGCAAGGC

TTGACCGACA

ATTGCATGAA

GAATCTGCTT

AGGGTTAGGC

7981

GTTTTGCGCT

GCTTCGCGAT

GTACGGGCCA

GATATACGCG

TTGACATTGA

TTATTGACTA

8041

GTTATTAATA

GTAATCAATT

ACGGGGTCAT

TAGTTCATAG

CCCATATATG

GAGTTCCGCG

8101

TTACATAACT

TACGGTAAAT

GGCCCGCCTG

GCTGACCGCC

CAACGACCCC

CGCCCATTGA

8161

CGTCAATAAT

GACGTATGTT

CCCATAGTAA

CGCCAATAGG

GACTTTCCAT

TGACGTCAAT

8221

GGGTGGACTA

TTTACGGTAA

ACTGCCCACT

TGGCAGTACA

TCAAGTGTAT

C

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