名称: | FokI-dCas9 |
---|---|
别称: | Plasmid#52970 |
基因长: | |
质粒宿主: | 哺乳细胞 |
质粒用途: | 基因编辑 |
片段类型: | CRISPR |
片段物种: | |
原核抗性: | Amp |
筛选标记: | |
荧光标记: | |
启动子: | |
复制子: | |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
ATATGCCAAG
TACGCCCCCT
ATTGACGTCA
ATGACGGTAA
ATGGCCCGCC
TGGCATTATG
CCCAGTACAT
GACCTTATGG
GACTTTCCTA
CTTGGCAGTA
CATCTACGTA
TTAGTCATCG
CTATTACCAT
GGTGATGCGG
TTTTGGCAGT
ACATCAATGG
GCGTGGATAG
CGGTTTGACT
CACGGGGATT
TCCAAGTCTC
CACCCCATTG
ACGTCAATGG
GAGTTTGTTT
TGGCACCAAA
ATCAACGGGA
CTTTCCAAAA
TGTCGTAACA
ACTCCGCCCC
ATTGACGCAA
ATGGGCGGTA
GGCGTGTACG
GTGGGAGGTC
TATATAAGCA
GAGCTGGTTT
AGTGAACCGT
CAGATCCGCT
AGAGATCCGC
GGCCGCTAAT
ACGACTCACT
ATAGGGAGAG
CCGCCACCAT
GGACTACAAA
GACCATGACG
GTGATTATAA
AGATCATGAC
ATCGATTACA
AGGATGACGA
TGACAAGATG
GCCCCCAAGA
AGAAGAGGAA
GGTGGGCATT
CACCGCGGGG
TACCTGGAGG
TTCTGGATCC
CAACTAGTCA
AAAGTGAACT
GGAGGAGAAG
AAATCTGAAC
TTCGTCATAA
ATTGAAATAT
GTGCCTCATG
AATATATTGA
ATTAATTGAA
ATTGCCAGAA
ATTCCACTCA
GGATAGAATT
CTTGAAATGA
AGGTAATGGA
ATTTTTTATG
AAAGTTTATG
GATATAGAGG
TAAACATTTG
GGTGGATCAA
GGAAACCGGA
CGGAGCAATT
TATACTGTCG
GATCTCCTAT
TGATTACGGT
GTGATCGTGG
ATACTAAAGC
TTATAGCGGA
GGTTATAATC
TGCCAATTGG
CCAAGCAGAT
GAAATGCAAC
GATATGTCGA
AGAAAATCAA
ACACGAAACA
AACATATCAA
CCCTAATGAA
TGGTGGAAAG
TCTATCCATC
TTCTGTAACG
GAATTTAAGT
TTTTATTTGT
GAGTGGTCAC
TTTAAAGGAA
ACTACAAAGC
TCAGCTTACA
CGATTAAATC
ATATCACTAA
TTGTAATGGA
GCTGTTCTTA
GTGTAGAAGA
GCTTTTAATT
GGTGGAGAAA
TGATTAAAGC
CGGCACATTA
ACCTTAGAGG
AAGTCAGACG
GAAATTTAAT
AACGGCGAGA
TAAACTTTAG
CGGCAGCGAG
ACTCCCGGGA
CCTCAGAGTC
CGCCACACCC
GAAAGTGATA
AAAAGTATTC
TATTGGTTTA
GCTATCGGCA
CTAATTCCGT
TGGATGGGCT
GTCATAACCG
ATGAATACAA
AGTACCTTCA
AAGAAATTTA
AGGTGTTGGG
GAACACAGAC
CGTCATTCGA
TTAAAAAGAA
TCTTATCGGT
GCCCTCCTAT
TCGATAGTGG
CGAAACGGCA
GAGGCGACTC
GCCTGAAACG
AACCGCTCGG
AGAAGGTATA
CACGTCGCAA
GAACCGAATA
TGTTACTTAC
AAGAAATTTT
TAGCAATGAG
ATGGCCAAAG
TTGACGATTC
TTTCTTTCAC
CGTTTGGAAG
AGTCCTTCCT
TGTCGAAGAG
GACAAGAAAC
ATGAACGGCA
CCCCATCTTT
GGAAACATAG
TAGATGAGGT
GGCATATCAT
GAAAAGTACC
CAACGATTTA
TCACCTCAGA
AAAAAGCTAG
TTGACTCAAC
TGATAAAGCG
GACCTGAGGT
TAATCTACTT
GGCTCTTGCC
CATATGATAA
AGTTCCGTGG
GCACTTTCTC
ATTGAGGGTG
ATCTAAATCC
GGACAACTCG
GATGTCGACA
AACTGTTCAT
CCAGTTAGTA
CAAACCTATA
ATCAGTTGTT
TGAAGAGAAC
CCTATAAATG
CAAGTGGCGT
GGATGCGAAG
GCTATTCTTA
GCGCCCGCCT
CTCTAAATCC
CGACGGCTAG
AAAACCTGAT
CGCACAATTA
CCCGGAGAGA
AGAAAAATGG
GTTGTTCGGT
AACCTTATAG
CGCTCTCACT
AGGCCTGACA
CCAAATTTTA
AGTCGAACTT
CGACTTAGCT
GAAGATGCCA
AATTGCAGCT
TAGTAAGGAC
ACGTACGATG
ACGATCTCGA
CAATCTACTG
GCACAAATTG
GAGATCAGTA
TGCGGACTTA
TTTTTGGCTG
CCAAAAACCT
TAGCGATGCA
ATCCTCCTAT
CTGACATACT
GAGAGTTAAT
ACTGAGATTA
CCAAGGCGCC
GTTATCCGCT
TCAATGATCA
AAAGGTACGA
TGAACATCAC
CAAGACTTGA
CACTTCTCAA
GGCCCTAGTC
CGTCAGCAAC
TGCCTGAGAA
ATATAAGGAA
ATATTCTTTG
ATCAGTCGAA
AAACGGGTAC
GCAGGTTATA
TTGACGGCGG
AGCGAGTCAA
GAGGAATTCT
ACAAGTTTAT
CAAACCCATA
TTAGAGAAGA
TGGATGGGAC
GGAAGAGTTG
CTTGTAAAAC
TCAATCGCGA
AGATCTACTG
CGAAAGCAGC
GGACTTTCGA
CAACGGTAGC
ATTCCACATC
AAATCCACTT
AGGCGAATTG
CATGCTATAC
TTAGAAGGCA
GGAGGATTTT
TATCCGTTCC
TCAAAGACAA
TCGTGAAAAG
ATTGAGAAAA
TCCTAACCTT
TCGCATACCT
TACTATGTGG
GACCCCTGGC
CCGAGGGAAC
TCTCGGTTCG
CATGGATGAC
AAGAAAGTCC
GAAGAAACGA
TTACTCCATG
GAATTTTGAG
GAAGTTGTCG
ATAAAGGTGC
GTCAGCTCAA
TCGTTCATCG
AGAGGATGAC
CAACTTTGAC
AAGAATTTAC
CGAACGAAAA
AGTATTGCCT
AAGCACAGTT
TACTTTACGA
GTATTTCACA
GTGTACAATG
AACTCACGAA
AGTTAAGTAT
GTCACTGAGG
GCATGCGTAA
ACCCGCCTTT
CTAAGCGGAG
AACAGAAGAA
AGCAATAGTA
GATCTGTTAT
TCAAGACCAA
CCGCAAAGTG
ACAGTTAAGC
AATTGAAAGA
GGACTACTTT
AAGAAAATTG
AATGCTTCGA
TTCTGTCGAG
ATCTCCGGGG
TAGAAGATCG
ATTTAATGCG
TCACTTGGTA
CGTATCATGA
CCTCCTAAAG
ATAATTAAAG
ATAAGGACTT
CCTGGATAAC
GAAGAGAATG
AAGATATCTT
AGAAGATATA
GTGTTGACTC
TTACCCTCTT
TGAAGATCGG
GAAATGATTG
AGGAAAGACT
AAAAACATAC
GCTCACCTGT
TCGACGATAA
GGTTATGAAA
CAGTTAAAGA
GGCGTCGCTA
TACGGGCTGG
GGACGATTGT
CGCGGAAACT
TATCAACGGG
ATAAGAGACA
AGCAAAGTGG
TAAAACTATT
CTCGATTTTC
TAAAGAGCGA
CGGCTTCGCC
AATAGGAACT
TTATGCAGCT
GATCCATGAT
GACTCTTTAA
CCTTCAAAGA
GGATATACAA
AAGGCACAGG
TTTCCGGACA
AGGGGACTCA
TTGCACGAAC
ATATTGCGAA
TCTTGCTGGT
TCGCCAGCCA
TCAAAAAGGG
CATACTCCAG
ACAGTCAAAG
TAGTGGATGA
GCTAGTTAAG
GTCATGGGAC
GTCACAAACC
GGAAAACATT
GTAATCGAGA
TGGCACGCGA
AAATCAAACG
ACTCAGAAGG
GGCAAAAAAA
CAGTCGAGAG
CGGATGAAGA
GAATAGAAGA
GGGTATTAAA
GAACTGGGCA
GCCAGATCTT
AAAGGAGCAT
CCTGTGGAAA
ATACCCAATT
GCAGAACGAG
AAACTTTACC
TCTATTACCT
ACAAAATGGA
AGGGACATGT
ATGTTGATCA
GGAACTGGAC
ATAAACCGTT
TATCTGATTA
CGACGTCGAT
GCCATTGTAC
CCCAATCCTT
TTTGAAGGAC
GATTCAATCG
ACAATAAAGT
GCTTACACGC
TCGGATAAGA
ACCGAGGGAA
AAGTGACAAT
GTTCCAAGCG
AGGAAGTCGT
AAAGAAAATG
AAGAACTATT
GGCGGCAGCT
CCTAAATGCG
AAACTGATAA
CGCAAAGAAA
GTTCGATAAC
TTAACTAAAG
CTGAGAGGGG
TGGCTTGTCT
GAACTTGACA
AGGCCGGATT
TATTAAACGT
CAGCTCGTGG
AAACCCGCCA
AATCACAAAG
CATGTTGCAC
AGATACTAGA
TTCCCGAATG
AATACGAAAT
ACGACGAGAA
CGATAAGCTG
ATTCGGGAAG
TCAAAGTAAT
CACTTTAAAG
TCAAAATTGG
TGTCGGACTT
CAGAAAGGAT
TTTCAATTCT
ATAAAGTTAG
GGAGATAAAT
AACTACCACC
ATGCGCACGA
CGCTTATCTT
AATGCCGTCG
TAGGGACCGC
ACTCATTAAG
AAATACCCGA
AGCTAGAAAG
TGAGTTTGTG
TATGGTGATT
ACAAAGTTTA
TGACGTCCGT
AAGATGATCG
CGAAAAGCGA
ACAGGAGATA
GGCAAGGCTA
CAGCCAAATA
CTTCTTTTAT
TCTAACATTA
TGAATTTCTT
TAAGACGGAA
ATCACTCTGG
CAAACGGAGA
GATACGCAAA
CGACCTTTAA
TTGAAACCAA
TGGGGAGACA
GGTGAAATCG
TATGGGATAA
GGGCCGGGAC
TTCGCGACGG
TGAGAAAAGT
TTTGTCCATG
CCCCAAGTCA
ACATAGTAAA
GAAAACTGAG
GTGCAGACCG
GAGGGTTTTC
AAAGGAATCG
ATTCTTCCAA
AAAGGAATAG
TGATAAGCTC
ATCGCTCGTA
AAAAGGACTG
GGACCCGAAA
AAGTACGGTG
GCTTCGATAG
CCCTACAGTT
GCCTATTCTG
TCCTAGTAGT
GGCAAAAGTT
GAGAAGGGAA
AATCCAAGAA
ACTGAAGTCA
GTCAAAGAAT
TATTGGGGAT
AACGATTATG
GAGCGCTCGT
CTTTTGAAAA
GAACCCCATC
GACTTCCTTG
AGGCGAAAGG
TTACAAGGAA
GTAAAAAAGG
ATCTCATAAT
TAAACTACCA
AAGTATAGTC
TGTTTGAGTT
AGAAAATGGC
CGAAAACGGA
TGTTGGCTAG
CGCCGGAGAG
CTTCAAAAGG
GGAACGAACT
CGCACTACCG
TCTAAATACG
TGAATTTCCT
GTATTTAGCG
TCCCATTACG
AGAAGTTGAA
AGGTTCACCT
GAAGATAACG
AACAGAAGCA
ACTTTTTGTT
GAGCAGCACA
AACATTATCT
CGACGAAATC
ATAGAGCAAA
TTTCGGAATT
CAGTAAGAGA
GTCATCCTAG
CTGATGCCAA
TCTGGACAAA
GTATTAAGCG
CATACAACAA
GCACAGGGAT
AAACCCATAC
GTGAGCAGGC
GGAAAATATT
ATCCATTTGT
TTACTCTTAC
CAACCTCGGC
GCTCCAGCCG
CATTCAAGTA
TTTTGACACA
ACGATAGATC
GCAAACGATA
CACTTCTACC
AAGGAGGTGC
TAGACGCGAC
ACTGATTCAC
CAATCCATCA
CGGGATTATA
TGAAACTCGG
ATAGATTTGT
CACAGCTTGG
GGGTGACTGA
CCGGTCATCA
TCACCATCAC
CATTGAGTTT
AAACCCGCTG
ATCAGCCTCG
ACTGTGCCTT
CTAGTTGCCA
GCCATCTGTT
GTTTGCCCCT
CCCCCGTGCC
TTCCTTGACC
CTGGAAGGTG
CCACTCCCAC
TGTCCTTTCC
TAATAAAATG
AGGAAATTGC
ATCGCATTGT
CTGAGTAGGT
GTCATTCTAT
TCTGGGGGGT
GGGGTGGGGC
AGGACAGCAA
GGGGGAGGAT
TGGGAAGACA
ATAGCAGGCA
TGCTGGGGAT
GCGGTGGGCT
CTATGGCTTC
TGAGGCGGAA
AGAACCAGCT
GGGGCTCGAT
ACCGTCGACC
TCTAGCTAGA
GCTTGGCGTA
ATCATGGTCA
TAGCTGTTTC
CTGTGTGAAA
TTGTTATCCG
CTCACAATTC
CACACAACAT
ACGAGCCGGA
AGCATAAAGT
GTAAAGCCTA
GGGTGCCTAA
TGAGTGAGCT
AACTCACATT
AATTGCGTTG
CGCTCACTGC
CCGCTTTCCA
GTCGGGAAAC
CTGTCGTGCC
AGCTGCATTA
ATGAATCGGC
CAACGCGCGG
GGAGAGGCGG
TTTGCGTATT
GGGCGCTCTT
CCGCTTCCTC
GCTCACTGAC
TCGCTGCGCT
CGGTCGTTCG
GCTGCGGCGA
GCGGTATCAG
CTCACTCAAA
GGCGGTAATA
CGGTTATCCA
CAGAATCAGG
GGATAACGCA
GGAAAGAACA
TGTGAGCAAA
AGGCCAGCAA
AAGGCCAGGA
ACCGTAAAAA
GGCCGCGTTG
CTGGCGTTTT
TCCATAGGCT
CCGCCCCCCT
GACGAGCATC
ACAAAAATCG
ACGCTCAAGT
CAGAGGTGGC
GAAACCCGAC
AGGACTATAA
AGATACCAGG
CGTTTCCCCC
TGGAAGCTCC
CTCGTGCGCT
CTCCTGTTCC
GACCCTGCCG
CTTACCGGAT
ACCTGTCCGC
CTTTCTCCCT
TCGGGAAGCG
TGGCGCTTTC
TCAATGCTCA
CGCTGTAGGT
ATCTCAGTTC
GGTGTAGGTC
GTTCGCTCCA
AGCTGGGCTG
TGTGCACGAA
CCCCCCGTTC
AGCCCGACCG
CTGCGCCTTA
TCCGGTAACT
ATCGTCTTGA
GTCCAACCCG
GTAAGACACG
ACTTATCGCC
ACTGGCAGCA
GCCACTGGTA
ACAGGATTAG
CAGAGCGAGG
TATGTAGGCG
GTGCTACAGA
GTTCTTGAAG
TGGTGGCCTA
ACTACGGCTA
CACTAGAAGG
ACAGTATTTG
GTATCTGCGC
TCTGCTGAAG
CCAGTTACCT
TCGGAAAAAG
AGTTGGTAGC
TCTTGATCCG
GCAAACAAAC
CACCGCTGGT
AGCGGTGGTT
TTTTTGTTTG
CAAGCAGCAG
ATTACGCGCA
GAAAAAAAGG
ATCTCAAGAA
GATCCTTTGA
TCTTTTCTAC
GGGGTCTGAC
GCTCAGTGGA
ACGAAAACTC
ACGTTAAGGG
ATTTTGGTCA
TGAGATTATC
AAAAAGGATC
TTCACCTAGA
TCCTTTTAAA
TTAAAAATGA
AGTTTTAAAT
CAATCTAAAG
TATATATGAG
TAAACTTGGT
CTGACAGTTA
CCAATGCTTA
ATCAGTGAGG
CACCTATCTC
AGCGATCTGT
CTATTTCGTT
CATCCATAGT
TGCCTGACTC
CCCGTCGTGT
AGATAACTAC
GATACGGGAG
GGCTTACCAT
CTGGCCCCAG
TGCTGCAATG
ATACCGCGAG
ACCCACGCTC
ACCGGCTCCA
GATTTATCAG
CAATAAACCA
GCCAGCCGGA
AGGGCCGAGC
GCAGAAGTGG
TCCTGCAACT
TTATCCGCCT
CCATCCAGTC
TATTAATTGT
TGCCGGGAAG
CTAGAGTAAG
TAGTTCGCCA
GTTAATAGTT
TGCGCAACGT
TGTTGCCATT
GCTACAGGCA
TCGTGGTGTC
ACGCTCGTCG
TTTGGTATGG
CTTCATTCAG
CTCCGGTTCC
CAACGATCAA
GGCGAGTTAC
ATGATCCCCC
ATGTTGTGCA
AAAAAGCGGT
TAGCTCCTTC
GGTCCTCCGA
TCGTTGTCAG
AAGTAAGTTG
GCCGCAGTGT
TATCACTCAT
GGTTATGGCA
GCACTGCATA
ATTCTCTTAC
TGTCATGCCA
TCCGTAAGAT
GCTTTTCTGT
GACTGGTGAG
TACTCAACCA
AGTCATTCTG
AGAATAGTGT
ATGCGGCGAC
CGAGTTGCTC
TTGCCCGGCG
TCAATACGGG
ATAATACCGC
GCCACATAGC
AGAACTTTAA
AAGTGCTCAT
CATTGGAAAA
CGTTCTTCGG
GGCGAAAACT
CTCAAGGATC
TTACCGCTGT
TGAGATCCAG
TTCGATGTAA
CCCACTCGTG
CACCCAACTG
ATCTTCAGCA
TCTTTTACTT
TCACCAGCGT
TTCTGGGTGA
GCAAAAACAG
GAAGGCAAAA
TGCCGCAAAA
AAGGGAATAA
GGGCGACACG
GAAATGTTGA
ATACTCATAC
TCTTCCTTTT
TCAATATTAT
TGAAGCATTT
ATCAGGGTTA
TTGTCTCATG
AGCGGATACA
TATTTGAATG
TATTTAGAAA
AATAAACAAA
TAGGGGTTCC
GCGCACATTT
CCCCGAAAAG
TGCCACCTGA
CGTCGACGGA
TCGGGAGATC
GATCTCCCGA
TCCCCTAGGG
TCGACTCTCA
GTACAATCTG
CTCTGATGCC
GCATAGTTAA
GCCAGTATCT
GCTCCCTGCT
TGTGTGTTGG
AGGTCGCTGA
GTAGTGCGCG
AGCAAAATTT
AAGCTACAAC
AAGGCAAGGC
TTGACCGACA
ATTGCATGAA
GAATCTGCTT
AGGGTTAGGC
GTTTTGCGCT
GCTTCGCGAT
GTACGGGCCA
GATATACGCG
TTGACATTGA
TTATTGACTA
GTTATTAATA
GTAATCAATT
ACGGGGTCAT
TAGTTCATAG
CCCATATATG
GAGTTCCGCG
TTACATAACT
TACGGTAAAT
GGCCCGCCTG
GCTGACCGCC
CAACGACCCC
CGCCCATTGA
CGTCAATAAT
GACGTATGTT
CCCATAGTAA
CGCCAATAGG
GACTTTCCAT
TGACGTCAAT
GGGTGGACTA
TTTACGGTAA
ACTGCCCACT
TGGCAGTACA
TCAAGTGTAT
C