名称: | pLV-5×NFKB-Fluc-Puro |
---|---|
别称: | |
基因长: | |
质粒宿主: | 哺乳细胞,慢病毒 |
质粒用途: | 转录激活 |
片段类型: | Element |
片段物种: | |
原核抗性: | Amp |
筛选标记: | Puro |
荧光标记: | Fluc |
启动子: | |
复制子: | pUC |
拷贝数: | |
感受态: | Stbl3 |
温度: | 37度 |
背景载体: | |
正向引物: | |
反向引物: | |
诱导方式: |
1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.
操作说明
一、质粒干粉(负20度保存,保质期90天,常温运输)
1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;
2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;
3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;
4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;
5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;
6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。
二、液体质粒(负20度保存,保质期90天,冰袋运输)
1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;
2、根据实验需求取适量质粒,其余保存于负20度冰箱。
三、甘油菌种(负80度保存,保质期90天,冰袋运输)
1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;
2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;
3、恒温培养箱倒置培养12~16h;
4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;
5、摇床震荡培养过夜,根据实验需要培养菌种。
CGGACCGCCA
CTGCCAATTA
CCTGTGGTTT
CATTTACTCT
AAACCTGTGA
TTCCTCTGAA
TTATTTTCAT
TTTAAAGAAA
TTGTATTTGT
TAAATATGTA
CTACAAACTT
AGTAGTTGGA
AGGGCTAATT
CACTCCCAAA
GAAGACAAGA
TATCCTTGAT
CTGTGGATCT
ACCACACACA
AGGCTACTTC
CCTGATTAGC
AGAACTACAC
ACCAGGGCCA
GGGGTCAGAT
ATCCACTGAC
CTTTGGATGG
TGCTACAAGC
TAGTACCAGT
TGAGCCAGAT
AAGGTAGAAG
AGGCCAATAA
AGGAGAGAAC
ACCAGCTTGT
TACACCCTGT
GAGCCTGCAT
GGGATGGATG
ACCCGGAGAG
AGAAGTGTTA
GAGTGGAGGT
TTGACAGCCG
CCTAGCATTT
CATCACGTGG
CCCGAGAGCT
GCATCCGGAG
TACTTCAAGA
ACTGCTGATA
TCGAGCTTGC
TACAAGGGAC
TTTCCGCTGG
GGACTTTCCA
GGGAGGCGTG
GCCTGGGCGG
GACTGGGGAG
TGGCGAGCCC
TCAGATCCTG
CATATAAGCA
GCTGCTTTTT
GCCTGTACTG
GGTCTCTCTG
GTTAGACCAG
ATCTGAGCCT
GGGAGCTCTC
TGGCTAACTA
GGGAACCCAC
TGCTTAAGCC
TCAATAAAGC
TTGCCTTGAG
TGCTTCAAGT
AGTGTGTGCC
CGTCTGTTGT
GTGACTCTGG
TAACTAGAGA
TCCCTCAGAC
CCTTTTAGTC
AGTGTGGAAA
ATCTCTAGCA
GTGGCGCCCG
AACAGGGACT
TGAAAGCGAA
AGGGAAACCA
GAGGAGCTCT
CTCGACGCAG
GACTCGGCTT
GCTGAAGCGC
GCACGGCAAG
AGGCGAGGGG
CGGCGACTGG
TGAGTACGCC
AAAAATTTTG
ACTAGCGGAG
GCTAGAAGGA
GAGAGATGGG
TGCGAGAGCG
TCAGTATTAA
GCGGGGGAGA
ATTAGATCGC
GATGGGAAAA
AATTCGGTTA
AGGCCAGGGG
GAAAGAAAAA
ATATAAATTA
AAACATATAG
TATGGGCAAG
CAGGGAGCTA
GAACGATTCG
CAGTTAATCC
TGGCCTGTTA
GAAACATCAG
AAGGCTGTAG
ACAAATACTG
GGACAGCTAC
AACCATCCCT
TCAGACAGGA
TCAGAAGAAC
TTAGATCATT
ATATAATACA
GTAGCAACCC
TCTATTGTGT
GCATCAAAGG
ATAGAGATAA
AAGACACCAA
GGAAGCTTTA
GACAAGATAG
AGGAAGAGCA
AAACAAAAGT
AAGACCACCG
CACAGCAAGC
GGCCGCTGAT
CTTCAGACCT
GGAGGAGGAG
ATATGAGGGA
CAATTGGAGA
AGTGAATTAT
ATAAATATAA
AGTAGTAAAA
ATTGAACCAT
TAGGAGTAGC
ACCCACCAAG
GCAAAGAGAA
GAGTGGTGCA
GAGAGAAAAA
AGAGCAGTGG
GAATAGGAGC
TTTGTTCCTT
GGGTTCTTGG
GAGCAGCAGG
AAGCACTATG
GGCGCAGCGT
CAATGACGCT
GACGGTACAG
GCCAGACAAT
TATTGTCTGG
TATAGTGCAG
CAGCAGAACA
ATTTGCTGAG
GGCTATTGAG
GCGCAACAGC
ATCTGTTGCA
ACTCACAGTC
TGGGGCATCA
AGCAGCTCCA
GGCAAGAATC
CTGGCTGTGG
AAAGATACCT
AAAGGATCAA
CAGCTCCTGG
GGATTTGGGG
TTGCTCTGGA
AAACTCATTT
GCACCACTGC
TGTGCCTTGG
AATGCTAGTT
GGAGTAATAA
ATCTCTGGAA
CAGATTTGGA
ATCACACGAC
CTGGATGGAG
TGGGACAGAG
AAATTAACAA
TTACACAAGC
TTAATACACT
CCTTAATTGA
AGAATCGCAA
AACCAGCAAG
AAAAGAATGA
ACAAGAATTA
TTGGAATTAG
ATAAATGGGC
AAGTTTGTGG
AATTGGTTTA
ACATAACAAA
TTGGCTGTGG
TATATAAAAT
TATTCATAAT
GATAGTAGGA
GGCTTGGTAG
GTTTAAGAAT
AGTTTTTGCT
GTACTTTCTA
TAGTGAATAG
AGTTAGGCAG
GGATATTCAC
CATTATCGTT
TCAGACCCAC
CTCCCAACCC
CGAGGGGACC
CGACAGGCCC
GAAGGAATAG
AAGAAGAAGG
TGGAGAGAGA
GACAGAGACA
GATCCATTCG
ATTAGTGAAC
GGATCTCGAC
GGTATCGCCA
CAAATGGCAG
TATTCATCCA
CAATTTTAAA
AGAAAGGGGG
GGATTGGGGG
GTACAGTGCA
GGGGAAAGAA
TAGTAGACAT
AATAGCAACA
GACATACAAA
CTAAAGAATT
ACAAAAACAA
ATTACAAAAA
TTCAAAATTT
TCGGGTTTAT
TACAGGGACA
GCAGAGATCC
ACTTTGGATC
GATAAGCTTT
GCAAAGATGG
ATAAAGTTTT
AAACAGAGAG
GAATCTTTGC
AGCTAATGGA
CCTTCTAGGT
CTTGAAAGGA
GTGGGAATTG
GATCCGGGGA
CTTTCCGCTT
GGGGACTTTC
CGCTGGGGAC
TTTCCGCTGG
GGACTTTCCG
CTGGGGACTT
TCCGAATTCA
GCTTGAATAA
AATGAATATT
AGAAGCTGTT
AGAATAAGAG
AAAATGACAG
AGGAAAACTG
AAAGGGAGAA
CTGAAAGTGG
GAAATTCCTC
TGAGGCAGAA
AGGACCATCC
CTTATAAATA
GCACAGGCCA
TGAAGGAAGA
TCATTCTCAC
TGCAGCCTTT
GACAGCCTTT
GCCTCATCTT
GAAGCTTCAC
CATGGAAGAC
GCCAAAAACA
TAAAGAAAGG
CCCGGCGCCA
TTCTATCCTC
TTGAGGATGG
AACCGCTGGA
GAGCAACTGC
ATAAGGCTAT
GAAGAGATAC
GCCCTGGTTC
CTGGAACAAT
TGCTTTTACA
GATGCACATA
TCGAGGTGAA
CATCACGTAC
GCGGAATACT
TCGAAATGTC
CGTTCGGTTG
GCAGAAGCTA
TGAAACGATA
TGGGCTGAAT
ACAAATCACA
GAATCGTCGT
ATGCAGTGAA
AACTCTCTTC
AATTCTTTAT
GCCGGTGTTG
GGCGCGTTAT
TTATCGGAGT
TGCAGTTGCG
CCCGCGAACG
ACATTTATAA
TGAACGTGAA
TTGCTCAACA
GTATGAACAT
TTCGCAGCCT
ACCGTAGTGT
TTGTTTCCAA
AAAGGGGTTG
CAAAAAATTT
TGAACGTGCA
AAAAAAATTA
CCAATAATCC
AGAAAATTAT
TATCATGGAT
TCTAAAACGG
ATTACCAGGG
ATTTCAGTCG
ATGTACACGT
TCGTCACATC
TCATCTACCT
CCCGGTTTTA
ATGAATACGA
TTTTGTACCA
GAGTCCTTTG
ATCGTGACAA
AACAATTGCA
CTGATAATGA
ACTCCTCTGG
ATCTACTGGG
TTACCTAAGG
GTGTGGCCCT
TCCGCATAGA
ACTGCCTGCG
TCAGATTCTC
GCATGCCAGA
GATCCTATTT
TTGGCAATCA
AATCATTCCG
GATACTGCGA
TTTTAAGTGT
TGTTCCATTC
CATCACGGTT
TTGGAATGTT
TACTACACTC
GGATATTTGA
TATGTGGATT
TCGAGTCGTC
TTAATGTATA
GATTTGAAGA
AGAGCTGTTT
TTACGATCCC
TTCAGGATTA
CAAAATTCAA
AGTGCGTTGC
TAGTACCAAC
CCTATTTTCA
TTCTTCGCCA
AAAGCACTCT
GATTGACAAA
TACGATTTAT
CTAATTTACA
CGAAATTGCT
TCTGGGGGCG
CACCTCTTTC
GAAAGAAGTC
GGGGAAGCGG
TTGCAAAACG
CTTCCATCTT
CCAGGGATAC
GACAAGGATA
TGGGCTCACT
GAGACTACAT
CAGCTATTCT
GATTACACCC
GAGGGGGATG
ATAAACCGGG
CGCGGTCGGT
AAAGTTGTTC
CATTTTTTGA
AGCGAAGGTT
GTGGATCTGG
ATACCGGGAA
AACGCTGGGC
GTTAATCAGA
GAGGCGAATT
ATGTGTCAGA
GGACCTATGA
TTATGTCCGG
TTATGTAAAC
AATCCGGAAG
CGACCAACGC
CTTGATTGAC
AAGGATGGAT
GGCTACATTC
TGGAGACATA
GCTTACTGGG
ACGAAGACGA
ACACTTCTTC
ATAGTTGACC
GCTTGAAGTC
TTTAATTAAA
TACAAAGGAT
ACCAGGTGGC
CCCCGCTGAA
TTGGAGTCGA
TATTGTTACA
ACACCCCAAC
ATCTTCGACG
CGGGCGTGGC
AGGTCTTCCC
GACGATGACG
CCGGTGAACT
TCCCGCCGCC
GTTGTTGTTT
TGGAGCACGG
AAAGACGATG
ACGGAAAAAG
AGATCGTGGA
TTACGTCGCC
AGTCAAGTAA
CAACCGCGAA
AAAGTTGCGC
GGAGGAGTTG
TGTTTGTGGA
CGAAGTACCG
AAAGGTCTTA
CCGGAAAACT
CGACGCAAGA
AAAATCAGAG
AGATCCTCAT
AAAGGCCAAG
AAGGGCGGAA
AGTCCAAATT
GTGACTAGTG
ATCCGCATGC
ATCTAGGGCG
GCCAATTCCG
CCCCTCTCCC
CCCCCCCCCT
CTCCCTCCCC
CCCCCCTAAC
GTTACTGGCC
GAAGCCGCTT
GGAATAAGGC
CGGTGTGCGT
TTGTCTATAT
GTTATTTTCC
ACCATATTGC
CGTCTTTTGG
CAATGTGAGG
GCCCGGAAAC
CTGGCCCTGT
CTTCTTGACG
AGCATTCCTA
GGGGTCTTTC
CCCTCTCGCC
AAAGGAATGC
AAGGTCTGTT
GAATGTCGTG
AAGGAAGCAG
TTCCTCTGGA
AGCTTCTTGA
AGACAAACAA
CGTCTGTAGC
GACCCTTTGC
AGGCAGCGGA
ACCCCCCACC
TGGCGACAGG
TGCCTCTGCG
GCCAAAAGCC
ACGTGTATAA
GATACACCTG
CAAAGGCGGC
ACAACCCCAG
TGCCACGTTG
TGAGTTGGAT
AGTTGTGGAA
AGAGTCAAAT
GGCTCTCCTC
AAGCGTATTC
AACAAGGGGC
TGAAGGATGC
CCAGAAGGTA
CCCCATTGTA
TGGGATCTGA
TCTGGGGCCT
CGGTGCACAT
GCTTTACATG
TGTTTAGTCG
AGGTTAAAAA
AACGTCTAGG
CCCCCCGAAC
CACGGGGACG
TGGTTTTCCT
TTGAAAAACA
CGATGATAAG
CTTGCCACAA
CCCACAAGGA
GACGACCTTC
CATGACCGAG
TACAAGCCCA
CGGTGCGCCT
CGCCACCCGC
GACGACGTCC
CCCGGGCCGT
ACGCACCCTC
GCCGCCGCGT
TCGCCGACTA
CCCCGCCACG
CGCCACACCG
TCGACCCGGA
CCGCCACATC
GAGCGGGTCA
CCGAGCTGCA
AGAACTCTTC
CTCACGCGCG
TCGGGCTCGA
CATCGGCAAG
GTGTGGGTCG
CGGACGACGG
CGCCGCGGTG
GCGGTCTGGA
CCACGCCGGA
GAGCGTCGAA
GCGGGGGCGG
TGTTCGCCGA
GATCGGCCCG
CGCATGGCCG
AGTTGAGCGG
TTCCCGGCTG
GCCGCGCAGC
AACAGATGGA
AGGCCTCCTG
GCGCCGCACC
GGCCCAAGGA
GCCCGCGTGG
TTCCTGGCCA
CCGTCGGCGT
CTCGCCCGAC
CACCAGGGCA
AGGGTCTGGG
CAGCGCCGTC
GTGCTCCCCG
GAGTGGAGGC
GGCCGAGCGC
GCCGGGGTGC
CCGCCTTCCT
GGAGACCTCC
GCGCCCCGCA
ACCTCCCCTT
CTACGAGCGG
CTCGGCTTCA
CCGTCACCGC
CGACGTCGAG
TGCCCGAAGG
ACCGCGCGAC
CTGGTGCATG
ACCCGCAAGC
CCGGTGCCTG
ATAATAGGCG
GCCGCTCGAG
ACCTAGAAAA
ACATGGAGCA
ATCACAAGTA
GCAATACAGC
AGCTACCAAT
GCTGATTGTG
CCTGGCTAGA
AGCACAAGAG
GAGGAGGAGG
TGGGTTTTCC
AGTCACACCT
CAGGTACCTT
TAAGACCAAT
GACTTACAAG
GCAGCTGTAG
ATCTTAGCCA
CTTTTTAAAA
GAAAAGGGGG
GACTGGAAGG
GCTAATTCAC
TCCCAACGAA
GACAAGATCT
GCTTTTTGCT
TGTACTGGGT
CTCTCTGGTT
AGACCAGATC
TGAGCCTGGG
AGCTCTCTGG
CTAACTAGGG
AACCCACTGC
TTAAGCCTCA
ATAAAGCTTG
CCTTGAGTGC
TTCAAGTAGT
GTGTGCCCGT
CTGTTGTGTG
ACTCTGGTAA
CTAGAGATCC
CTCAGACCCT
TTTAGTCAGT
GTGGAAAATC
TCTAGCAGTA
GTAGTTCATG
TCATCTTATT
ATTCAGTATT
TATAACTTGC
AAAGAAATGA
ATATCAGAGA
GTGAGAGGCC
TTGACATTAT
AATAGATTTA
GCAGGAATTG
AACTAGGAGT
GGAGCACACA
GGCAAAGTTC
TAGAGCTCGC
TGATCAGCCT
CGACTGTGCC
TTCTAGTTGC
CAGCCATCTG
TTGTTTGCCC
CTCCCCCGTG
CCTTCCTTGA
CCCTGGAAGG
TGCCACTCCC
ACTGTCCTTT
CCTAATAAAA
TGAGGAAATT
GCATCGCATT
GTCTGAGTAG
GTGTCATTCT
ATTCTGGGGG
GTGGGGTGGG
GCAGGACAGC
AAGGGGGAGG
ATTGGGAAGA
CAATAGCAGG
CATGCTGGGG
ATGCGGTGGG
CTCTATGGCT
TCTGAGGCGG
AAAGAACCAG
CTGGGGGCGC
GCCCCTCGAG
GCCGCCATGG
TCATAGCTGT
TTGACGTCAG
GTGGCACTTT
TCGGGGAAAT
GTGCGCGGAA
CCCCTATTTG
TTTATTTTTC
TAAATACATT
CAAATATGTA
TCCGCTCATG
AGACAATAAC
CCTGATAAAT
GCTTCAATAA
TATTGAAAAA
GGAAGAGTAT
GAGTATTCAA
CATTTCCGTG
TCGCCCTTAT
TCCCTTTTTT
GCGGCATTTT
GCCTTCCTGT
TTTTGCTCAC
CCAGAAACGC
TGGTGAAAGT
AAAAGATGCT
GAAGATCAGT
TGGGTGCACG
AGTGGGTTAC
ATCGAACTGG
ATCTCAACAG
CGGTAAGATC
CTTGAGAGTT
TTCGCCCCGA
AGAACGTTTT
CCAATGATGA
GCACTTTTAA
AGTTCTGCTA
TGTGGCGCGG
TATTATCCCG
TATTGACGCC
GGGCAAGAGC
AACTCGGTCG
CCGCATACAC
TATTCTCAGA
ATGACTTGGT
TGAGTACTCA
CCAGTCACAG
AAAAGCATCT
TACGGATGGC
ATGACAGTAA
GAGAATTATG
CAGTGCTGCC
ATAACCATGA
GTGATAACAC
TGCGGCCAAC
TTACTTCTGA
CAACGATCGG
AGGACCGAAG
GAGCTAACCG
CTTTTTTGCA
CAACATGGGG
GATCATGTAA
CTCGCCTTGA
TCGTTGGGAA
CCGGAGCTGA
ATGAAGCCAT
ACCAAACGAC
GAGCGTGACA
CCACGATGCC
TGTAGCAATG
GCAACAACGT
TGCGCAAACT
ATTAACTGGC
GAACTACTTA
CTCTAGCTTC
CCGGCAACAA
TTAATAGACT
GGATGGAGGC
GGATAAAGTT
GCAGGACCAC
TTCTGCGCTC
GGCCCTTCCG
GCTGGCTGGT
TTATTGCTGA
TAAATCTGGA
GCCGGTGAGC
GTGGGTCTCG
CGGTATCATT
GCAGCACTGG
GGCCAGATGG
TAAGCCCTCC
CGTATCGTAG
TTATCTACAC
GACGGGGAGT
CAGGCAACTA
TGGATGAACG
AAATAGACAG
ATCGCTGAGA
TAGGTGCCTC
ACTGATTAAG
CATTGGTAAC
TGTCAGACCA
AGTTTACTCA
TATATACTTT
AGATTGATTT
AAAACTTCAT
TTTTAATTTA
AAAGGATCTA
GGTGAAGATC
CTTTTTGATA
ATCTCATGAC
CAAAATCCCT
TAACGTGAGT
TTTCGTTCCA
CTGAGCGTCA
GACCCCGTAG
AAAAGATCAA
AGGATCTTCT
TGAGATCCTT
TTTTTCTGCG
CGTAATCTGC
TGCTTGCAAA
CAAAAAAACC
ACCGCTACCA
GCGGTGGTTT
GTTTGCCGGA
TCAAGAGCTA
CCAACTCTTT
TTCCGAAGGT
AACTGGCTTC
AGCAGAGCGC
AGATACCAAA
TACTGTCCTT
CTAGTGTAGC
CGTAGTTAGG
CCACCACTTC
AAGAACTCTG
TAGCACCGCC
TACATACCTC
GCTCTGCTAA
TCCTGTTACC
AGTGGCTGCT
GCCAGTGGCG
ATAAGTCGTG
TCTTACCGGG
TTGGACTCAA
GACGATAGTT
ACCGGATAAG
GCGCAGCGGT
CGGGCTGAAC
GGGGGGTTCG
TGCACACAGC
CCAGCTTGGA
GCGAACGACC
TACACCGAAC
TGAGATACCT
ACAGCGTGAG
CATTGAGAAA
GCGCCACGCT
TCCCGAAGGG
AGAAAGGCGG
ACAGGTATCC
GGTAAGCGGC
AGGGTCGGAA
CAGGAGAGCG
CACGAGGGAG
CTTCCAGGGG
GAAACGCCTG
GTATCTTTAT
AGTCCTGTCG
GGTTTCGCCA
CCTCTGACTT
GAGCGTCGAT
TTTTGTGATG
CTCGTCAGGG
GGGCGGAGCC
TATGGAAAAA
CGCCAGCAAC
GCGGCCTTTT
TACGGTTCCT
GGCCTTTTGC