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pLV-5×NFKB-Fluc-Puro

产品货号:P50251

产品规格:

    少量干粉质粒 大提液体质粒

产品数量:

- 1 +

产品价格:0.00

一键复制产品信息
名称: pLV-5×NFKB-Fluc-Puro
别称:
基因长:
质粒宿主: 哺乳细胞,慢病毒
质粒用途: 转录激活
片段类型: Element
片段物种:
原核抗性: Amp
筛选标记: Puro
荧光标记: Fluc
启动子:
复制子: pUC
拷贝数:
感受态: Stbl3
温度: 37度
背景载体:
正向引物:
反向引物:
诱导方式:

1、本平台所有产品仅供科研使用,不能用于临床试验,包括人体口服、注射或者体外用药;
2、小包装质粒以模板价格出售,只保证基因部分测序正确,不保证质粒的表达等实验效果;
3、某些质粒即使是出品公司亦没有完整测过序,如关键部位的序列测序正确,亦认为是正确质粒;
4、小包装质粒产品的售后期为您收到产品之日起3个月,收到质粒后请及时转化操作,过期需重新购买;
5、质粒构建皆收取的是成本费用,所有质粒会分享给其他实验者,保密性质粒请提前告知并缴纳保密费用;
6、因科学研究为探索未知的活动,存在较大不确定性,故在任何情况下淼灵都不承担额外的连带责任;
7、发表[中文论文]请标注:产品名称(产品货号) 由武汉淼灵生物科技有限公司提供;
8、发表[英文论文]请标注:Product Name (Product Item Number) was obtained from MiaoLingBio,China.

操作说明

一、质粒干粉(负20度保存,保质期90天,常温运输)

1、收到干粉质粒后请先5000rpm离心1min,再加入20μl无菌水溶解质粒;

2、将相应的感受态于冰盒上解冻,取2μl质粒加至100μl感受态中,冰浴 30min;

3、42度热激60s,冰浴2min,加入900μl无抗LB培养基,180rpm震荡培养45min;

4、6000rpm离心3min,留100μl上清重悬,再涂至对应抗性的LB平板上;

5、倒置培养过夜后,如菌落过多则稀释质粒转化,如没有菌落则取10ul质粒转化;

6、挑单菌落至对应抗性的LB液体培养基中过夜震荡培养,根据需要提取质粒。


二、液体质粒(负20度保存,保质期90天,冰袋运输)

1、收到液体质粒后请甩动保存管,让液体质粒不要吸附在管壁上;

2、根据实验需求取适量质粒,其余保存于负20度冰箱。

三、甘油菌种(负80度保存,保质期90天,冰袋运输)

1、收到甘油菌种后请甩动保存管,让菌液不要吸附在管壁上;

2、用接种环在固体平板上四区划线,如有抗性就加对应抗性;

3、恒温培养箱倒置培养12~16h;

4、务必挑取单菌落接种于液体培养基中,如有抗性就加对应抗性;

5、摇床震荡培养过夜,根据实验需要培养菌种。

一键复制序列(有部分质粒是非全长序列)
1

CGGACCGCCA

CTGCCAATTA

CCTGTGGTTT

CATTTACTCT

AAACCTGTGA

TTCCTCTGAA

61

TTATTTTCAT

TTTAAAGAAA

TTGTATTTGT

TAAATATGTA

CTACAAACTT

AGTAGTTGGA

121

AGGGCTAATT

CACTCCCAAA

GAAGACAAGA

TATCCTTGAT

CTGTGGATCT

ACCACACACA

181

AGGCTACTTC

CCTGATTAGC

AGAACTACAC

ACCAGGGCCA

GGGGTCAGAT

ATCCACTGAC

241

CTTTGGATGG

TGCTACAAGC

TAGTACCAGT

TGAGCCAGAT

AAGGTAGAAG

AGGCCAATAA

301

AGGAGAGAAC

ACCAGCTTGT

TACACCCTGT

GAGCCTGCAT

GGGATGGATG

ACCCGGAGAG

361

AGAAGTGTTA

GAGTGGAGGT

TTGACAGCCG

CCTAGCATTT

CATCACGTGG

CCCGAGAGCT

421

GCATCCGGAG

TACTTCAAGA

ACTGCTGATA

TCGAGCTTGC

TACAAGGGAC

TTTCCGCTGG

481

GGACTTTCCA

GGGAGGCGTG

GCCTGGGCGG

GACTGGGGAG

TGGCGAGCCC

TCAGATCCTG

541

CATATAAGCA

GCTGCTTTTT

GCCTGTACTG

GGTCTCTCTG

GTTAGACCAG

ATCTGAGCCT

601

GGGAGCTCTC

TGGCTAACTA

GGGAACCCAC

TGCTTAAGCC

TCAATAAAGC

TTGCCTTGAG

661

TGCTTCAAGT

AGTGTGTGCC

CGTCTGTTGT

GTGACTCTGG

TAACTAGAGA

TCCCTCAGAC

721

CCTTTTAGTC

AGTGTGGAAA

ATCTCTAGCA

GTGGCGCCCG

AACAGGGACT

TGAAAGCGAA

781

AGGGAAACCA

GAGGAGCTCT

CTCGACGCAG

GACTCGGCTT

GCTGAAGCGC

GCACGGCAAG

841

AGGCGAGGGG

CGGCGACTGG

TGAGTACGCC

AAAAATTTTG

ACTAGCGGAG

GCTAGAAGGA

901

GAGAGATGGG

TGCGAGAGCG

TCAGTATTAA

GCGGGGGAGA

ATTAGATCGC

GATGGGAAAA

961

AATTCGGTTA

AGGCCAGGGG

GAAAGAAAAA

ATATAAATTA

AAACATATAG

TATGGGCAAG

1021

CAGGGAGCTA

GAACGATTCG

CAGTTAATCC

TGGCCTGTTA

GAAACATCAG

AAGGCTGTAG

1081

ACAAATACTG

GGACAGCTAC

AACCATCCCT

TCAGACAGGA

TCAGAAGAAC

TTAGATCATT

1141

ATATAATACA

GTAGCAACCC

TCTATTGTGT

GCATCAAAGG

ATAGAGATAA

AAGACACCAA

1201

GGAAGCTTTA

GACAAGATAG

AGGAAGAGCA

AAACAAAAGT

AAGACCACCG

CACAGCAAGC

1261

GGCCGCTGAT

CTTCAGACCT

GGAGGAGGAG

ATATGAGGGA

CAATTGGAGA

AGTGAATTAT

1321

ATAAATATAA

AGTAGTAAAA

ATTGAACCAT

TAGGAGTAGC

ACCCACCAAG

GCAAAGAGAA

1381

GAGTGGTGCA

GAGAGAAAAA

AGAGCAGTGG

GAATAGGAGC

TTTGTTCCTT

GGGTTCTTGG

1441

GAGCAGCAGG

AAGCACTATG

GGCGCAGCGT

CAATGACGCT

GACGGTACAG

GCCAGACAAT

1501

TATTGTCTGG

TATAGTGCAG

CAGCAGAACA

ATTTGCTGAG

GGCTATTGAG

GCGCAACAGC

1561

ATCTGTTGCA

ACTCACAGTC

TGGGGCATCA

AGCAGCTCCA

GGCAAGAATC

CTGGCTGTGG

1621

AAAGATACCT

AAAGGATCAA

CAGCTCCTGG

GGATTTGGGG

TTGCTCTGGA

AAACTCATTT

1681

GCACCACTGC

TGTGCCTTGG

AATGCTAGTT

GGAGTAATAA

ATCTCTGGAA

CAGATTTGGA

1741

ATCACACGAC

CTGGATGGAG

TGGGACAGAG

AAATTAACAA

TTACACAAGC

TTAATACACT

1801

CCTTAATTGA

AGAATCGCAA

AACCAGCAAG

AAAAGAATGA

ACAAGAATTA

TTGGAATTAG

1861

ATAAATGGGC

AAGTTTGTGG

AATTGGTTTA

ACATAACAAA

TTGGCTGTGG

TATATAAAAT

1921

TATTCATAAT

GATAGTAGGA

GGCTTGGTAG

GTTTAAGAAT

AGTTTTTGCT

GTACTTTCTA

1981

TAGTGAATAG

AGTTAGGCAG

GGATATTCAC

CATTATCGTT

TCAGACCCAC

CTCCCAACCC

2041

CGAGGGGACC

CGACAGGCCC

GAAGGAATAG

AAGAAGAAGG

TGGAGAGAGA

GACAGAGACA

2101

GATCCATTCG

ATTAGTGAAC

GGATCTCGAC

GGTATCGCCA

CAAATGGCAG

TATTCATCCA

2161

CAATTTTAAA

AGAAAGGGGG

GGATTGGGGG

GTACAGTGCA

GGGGAAAGAA

TAGTAGACAT

2221

AATAGCAACA

GACATACAAA

CTAAAGAATT

ACAAAAACAA

ATTACAAAAA

TTCAAAATTT

2281

TCGGGTTTAT

TACAGGGACA

GCAGAGATCC

ACTTTGGATC

GATAAGCTTT

GCAAAGATGG

2341

ATAAAGTTTT

AAACAGAGAG

GAATCTTTGC

AGCTAATGGA

CCTTCTAGGT

CTTGAAAGGA

2401

GTGGGAATTG

GATCCGGGGA

CTTTCCGCTT

GGGGACTTTC

CGCTGGGGAC

TTTCCGCTGG

2461

GGACTTTCCG

CTGGGGACTT

TCCGAATTCA

GCTTGAATAA

AATGAATATT

AGAAGCTGTT

2521

AGAATAAGAG

AAAATGACAG

AGGAAAACTG

AAAGGGAGAA

CTGAAAGTGG

GAAATTCCTC

2581

TGAGGCAGAA

AGGACCATCC

CTTATAAATA

GCACAGGCCA

TGAAGGAAGA

TCATTCTCAC

2641

TGCAGCCTTT

GACAGCCTTT

GCCTCATCTT

GAAGCTTCAC

CATGGAAGAC

GCCAAAAACA

2701

TAAAGAAAGG

CCCGGCGCCA

TTCTATCCTC

TTGAGGATGG

AACCGCTGGA

GAGCAACTGC

2761

ATAAGGCTAT

GAAGAGATAC

GCCCTGGTTC

CTGGAACAAT

TGCTTTTACA

GATGCACATA

2821

TCGAGGTGAA

CATCACGTAC

GCGGAATACT

TCGAAATGTC

CGTTCGGTTG

GCAGAAGCTA

2881

TGAAACGATA

TGGGCTGAAT

ACAAATCACA

GAATCGTCGT

ATGCAGTGAA

AACTCTCTTC

2941

AATTCTTTAT

GCCGGTGTTG

GGCGCGTTAT

TTATCGGAGT

TGCAGTTGCG

CCCGCGAACG

3001

ACATTTATAA

TGAACGTGAA

TTGCTCAACA

GTATGAACAT

TTCGCAGCCT

ACCGTAGTGT

3061

TTGTTTCCAA

AAAGGGGTTG

CAAAAAATTT

TGAACGTGCA

AAAAAAATTA

CCAATAATCC

3121

AGAAAATTAT

TATCATGGAT

TCTAAAACGG

ATTACCAGGG

ATTTCAGTCG

ATGTACACGT

3181

TCGTCACATC

TCATCTACCT

CCCGGTTTTA

ATGAATACGA

TTTTGTACCA

GAGTCCTTTG

3241

ATCGTGACAA

AACAATTGCA

CTGATAATGA

ACTCCTCTGG

ATCTACTGGG

TTACCTAAGG

3301

GTGTGGCCCT

TCCGCATAGA

ACTGCCTGCG

TCAGATTCTC

GCATGCCAGA

GATCCTATTT

3361

TTGGCAATCA

AATCATTCCG

GATACTGCGA

TTTTAAGTGT

TGTTCCATTC

CATCACGGTT

3421

TTGGAATGTT

TACTACACTC

GGATATTTGA

TATGTGGATT

TCGAGTCGTC

TTAATGTATA

3481

GATTTGAAGA

AGAGCTGTTT

TTACGATCCC

TTCAGGATTA

CAAAATTCAA

AGTGCGTTGC

3541

TAGTACCAAC

CCTATTTTCA

TTCTTCGCCA

AAAGCACTCT

GATTGACAAA

TACGATTTAT

3601

CTAATTTACA

CGAAATTGCT

TCTGGGGGCG

CACCTCTTTC

GAAAGAAGTC

GGGGAAGCGG

3661

TTGCAAAACG

CTTCCATCTT

CCAGGGATAC

GACAAGGATA

TGGGCTCACT

GAGACTACAT

3721

CAGCTATTCT

GATTACACCC

GAGGGGGATG

ATAAACCGGG

CGCGGTCGGT

AAAGTTGTTC

3781

CATTTTTTGA

AGCGAAGGTT

GTGGATCTGG

ATACCGGGAA

AACGCTGGGC

GTTAATCAGA

3841

GAGGCGAATT

ATGTGTCAGA

GGACCTATGA

TTATGTCCGG

TTATGTAAAC

AATCCGGAAG

3901

CGACCAACGC

CTTGATTGAC

AAGGATGGAT

GGCTACATTC

TGGAGACATA

GCTTACTGGG

3961

ACGAAGACGA

ACACTTCTTC

ATAGTTGACC

GCTTGAAGTC

TTTAATTAAA

TACAAAGGAT

4021

ACCAGGTGGC

CCCCGCTGAA

TTGGAGTCGA

TATTGTTACA

ACACCCCAAC

ATCTTCGACG

4081

CGGGCGTGGC

AGGTCTTCCC

GACGATGACG

CCGGTGAACT

TCCCGCCGCC

GTTGTTGTTT

4141

TGGAGCACGG

AAAGACGATG

ACGGAAAAAG

AGATCGTGGA

TTACGTCGCC

AGTCAAGTAA

4201

CAACCGCGAA

AAAGTTGCGC

GGAGGAGTTG

TGTTTGTGGA

CGAAGTACCG

AAAGGTCTTA

4261

CCGGAAAACT

CGACGCAAGA

AAAATCAGAG

AGATCCTCAT

AAAGGCCAAG

AAGGGCGGAA

4321

AGTCCAAATT

GTGACTAGTG

ATCCGCATGC

ATCTAGGGCG

GCCAATTCCG

CCCCTCTCCC

4381

CCCCCCCCCT

CTCCCTCCCC

CCCCCCTAAC

GTTACTGGCC

GAAGCCGCTT

GGAATAAGGC

4441

CGGTGTGCGT

TTGTCTATAT

GTTATTTTCC

ACCATATTGC

CGTCTTTTGG

CAATGTGAGG

4501

GCCCGGAAAC

CTGGCCCTGT

CTTCTTGACG

AGCATTCCTA

GGGGTCTTTC

CCCTCTCGCC

4561

AAAGGAATGC

AAGGTCTGTT

GAATGTCGTG

AAGGAAGCAG

TTCCTCTGGA

AGCTTCTTGA

4621

AGACAAACAA

CGTCTGTAGC

GACCCTTTGC

AGGCAGCGGA

ACCCCCCACC

TGGCGACAGG

4681

TGCCTCTGCG

GCCAAAAGCC

ACGTGTATAA

GATACACCTG

CAAAGGCGGC

ACAACCCCAG

4741

TGCCACGTTG

TGAGTTGGAT

AGTTGTGGAA

AGAGTCAAAT

GGCTCTCCTC

AAGCGTATTC

4801

AACAAGGGGC

TGAAGGATGC

CCAGAAGGTA

CCCCATTGTA

TGGGATCTGA

TCTGGGGCCT

4861

CGGTGCACAT

GCTTTACATG

TGTTTAGTCG

AGGTTAAAAA

AACGTCTAGG

CCCCCCGAAC

4921

CACGGGGACG

TGGTTTTCCT

TTGAAAAACA

CGATGATAAG

CTTGCCACAA

CCCACAAGGA

4981

GACGACCTTC

CATGACCGAG

TACAAGCCCA

CGGTGCGCCT

CGCCACCCGC

GACGACGTCC

5041

CCCGGGCCGT

ACGCACCCTC

GCCGCCGCGT

TCGCCGACTA

CCCCGCCACG

CGCCACACCG

5101

TCGACCCGGA

CCGCCACATC

GAGCGGGTCA

CCGAGCTGCA

AGAACTCTTC

CTCACGCGCG

5161

TCGGGCTCGA

CATCGGCAAG

GTGTGGGTCG

CGGACGACGG

CGCCGCGGTG

GCGGTCTGGA

5221

CCACGCCGGA

GAGCGTCGAA

GCGGGGGCGG

TGTTCGCCGA

GATCGGCCCG

CGCATGGCCG

5281

AGTTGAGCGG

TTCCCGGCTG

GCCGCGCAGC

AACAGATGGA

AGGCCTCCTG

GCGCCGCACC

5341

GGCCCAAGGA

GCCCGCGTGG

TTCCTGGCCA

CCGTCGGCGT

CTCGCCCGAC

CACCAGGGCA

5401

AGGGTCTGGG

CAGCGCCGTC

GTGCTCCCCG

GAGTGGAGGC

GGCCGAGCGC

GCCGGGGTGC

5461

CCGCCTTCCT

GGAGACCTCC

GCGCCCCGCA

ACCTCCCCTT

CTACGAGCGG

CTCGGCTTCA

5521

CCGTCACCGC

CGACGTCGAG

TGCCCGAAGG

ACCGCGCGAC

CTGGTGCATG

ACCCGCAAGC

5581

CCGGTGCCTG

ATAATAGGCG

GCCGCTCGAG

ACCTAGAAAA

ACATGGAGCA

ATCACAAGTA

5641

GCAATACAGC

AGCTACCAAT

GCTGATTGTG

CCTGGCTAGA

AGCACAAGAG

GAGGAGGAGG

5701

TGGGTTTTCC

AGTCACACCT

CAGGTACCTT

TAAGACCAAT

GACTTACAAG

GCAGCTGTAG

5761

ATCTTAGCCA

CTTTTTAAAA

GAAAAGGGGG

GACTGGAAGG

GCTAATTCAC

TCCCAACGAA

5821

GACAAGATCT

GCTTTTTGCT

TGTACTGGGT

CTCTCTGGTT

AGACCAGATC

TGAGCCTGGG

5881

AGCTCTCTGG

CTAACTAGGG

AACCCACTGC

TTAAGCCTCA

ATAAAGCTTG

CCTTGAGTGC

5941

TTCAAGTAGT

GTGTGCCCGT

CTGTTGTGTG

ACTCTGGTAA

CTAGAGATCC

CTCAGACCCT

6001

TTTAGTCAGT

GTGGAAAATC

TCTAGCAGTA

GTAGTTCATG

TCATCTTATT

ATTCAGTATT

6061

TATAACTTGC

AAAGAAATGA

ATATCAGAGA

GTGAGAGGCC

TTGACATTAT

AATAGATTTA

6121

GCAGGAATTG

AACTAGGAGT

GGAGCACACA

GGCAAAGTTC

TAGAGCTCGC

TGATCAGCCT

6181

CGACTGTGCC

TTCTAGTTGC

CAGCCATCTG

TTGTTTGCCC

CTCCCCCGTG

CCTTCCTTGA

6241

CCCTGGAAGG

TGCCACTCCC

ACTGTCCTTT

CCTAATAAAA

TGAGGAAATT

GCATCGCATT

6301

GTCTGAGTAG

GTGTCATTCT

ATTCTGGGGG

GTGGGGTGGG

GCAGGACAGC

AAGGGGGAGG

6361

ATTGGGAAGA

CAATAGCAGG

CATGCTGGGG

ATGCGGTGGG

CTCTATGGCT

TCTGAGGCGG

6421

AAAGAACCAG

CTGGGGGCGC

GCCCCTCGAG

GCCGCCATGG

TCATAGCTGT

TTGACGTCAG

6481

GTGGCACTTT

TCGGGGAAAT

GTGCGCGGAA

CCCCTATTTG

TTTATTTTTC

TAAATACATT

6541

CAAATATGTA

TCCGCTCATG

AGACAATAAC

CCTGATAAAT

GCTTCAATAA

TATTGAAAAA

6601

GGAAGAGTAT

GAGTATTCAA

CATTTCCGTG

TCGCCCTTAT

TCCCTTTTTT

GCGGCATTTT

6661

GCCTTCCTGT

TTTTGCTCAC

CCAGAAACGC

TGGTGAAAGT

AAAAGATGCT

GAAGATCAGT

6721

TGGGTGCACG

AGTGGGTTAC

ATCGAACTGG

ATCTCAACAG

CGGTAAGATC

CTTGAGAGTT

6781

TTCGCCCCGA

AGAACGTTTT

CCAATGATGA

GCACTTTTAA

AGTTCTGCTA

TGTGGCGCGG

6841

TATTATCCCG

TATTGACGCC

GGGCAAGAGC

AACTCGGTCG

CCGCATACAC

TATTCTCAGA

6901

ATGACTTGGT

TGAGTACTCA

CCAGTCACAG

AAAAGCATCT

TACGGATGGC

ATGACAGTAA

6961

GAGAATTATG

CAGTGCTGCC

ATAACCATGA

GTGATAACAC

TGCGGCCAAC

TTACTTCTGA

7021

CAACGATCGG

AGGACCGAAG

GAGCTAACCG

CTTTTTTGCA

CAACATGGGG

GATCATGTAA

7081

CTCGCCTTGA

TCGTTGGGAA

CCGGAGCTGA

ATGAAGCCAT

ACCAAACGAC

GAGCGTGACA

7141

CCACGATGCC

TGTAGCAATG

GCAACAACGT

TGCGCAAACT

ATTAACTGGC

GAACTACTTA

7201

CTCTAGCTTC

CCGGCAACAA

TTAATAGACT

GGATGGAGGC

GGATAAAGTT

GCAGGACCAC

7261

TTCTGCGCTC

GGCCCTTCCG

GCTGGCTGGT

TTATTGCTGA

TAAATCTGGA

GCCGGTGAGC

7321

GTGGGTCTCG

CGGTATCATT

GCAGCACTGG

GGCCAGATGG

TAAGCCCTCC

CGTATCGTAG

7381

TTATCTACAC

GACGGGGAGT

CAGGCAACTA

TGGATGAACG

AAATAGACAG

ATCGCTGAGA

7441

TAGGTGCCTC

ACTGATTAAG

CATTGGTAAC

TGTCAGACCA

AGTTTACTCA

TATATACTTT

7501

AGATTGATTT

AAAACTTCAT

TTTTAATTTA

AAAGGATCTA

GGTGAAGATC

CTTTTTGATA

7561

ATCTCATGAC

CAAAATCCCT

TAACGTGAGT

TTTCGTTCCA

CTGAGCGTCA

GACCCCGTAG

7621

AAAAGATCAA

AGGATCTTCT

TGAGATCCTT

TTTTTCTGCG

CGTAATCTGC

TGCTTGCAAA

7681

CAAAAAAACC

ACCGCTACCA

GCGGTGGTTT

GTTTGCCGGA

TCAAGAGCTA

CCAACTCTTT

7741

TTCCGAAGGT

AACTGGCTTC

AGCAGAGCGC

AGATACCAAA

TACTGTCCTT

CTAGTGTAGC

7801

CGTAGTTAGG

CCACCACTTC

AAGAACTCTG

TAGCACCGCC

TACATACCTC

GCTCTGCTAA

7861

TCCTGTTACC

AGTGGCTGCT

GCCAGTGGCG

ATAAGTCGTG

TCTTACCGGG

TTGGACTCAA

7921

GACGATAGTT

ACCGGATAAG

GCGCAGCGGT

CGGGCTGAAC

GGGGGGTTCG

TGCACACAGC

7981

CCAGCTTGGA

GCGAACGACC

TACACCGAAC

TGAGATACCT

ACAGCGTGAG

CATTGAGAAA

8041

GCGCCACGCT

TCCCGAAGGG

AGAAAGGCGG

ACAGGTATCC

GGTAAGCGGC

AGGGTCGGAA

8101

CAGGAGAGCG

CACGAGGGAG

CTTCCAGGGG

GAAACGCCTG

GTATCTTTAT

AGTCCTGTCG

8161

GGTTTCGCCA

CCTCTGACTT

GAGCGTCGAT

TTTTGTGATG

CTCGTCAGGG

GGGCGGAGCC

8221

TATGGAAAAA

CGCCAGCAAC

GCGGCCTTTT

TACGGTTCCT

GGCCTTTTGC

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